AlfalfaGEDB | MS.gene21434

M. sativa cultivar XinJiangDaYe / MS.gene21434

KEGG: K08341
GO: -
PFAM: PF02991.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21434.t1	XP_003591331.1	79.2	149	3	1	1	149	1	121	2.80E-56	228

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21434.t1	Q8S925	72.4	105	29	0	42	146	15	119	9.3e-40	164.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21434.t1	G7IBT1	79.2	149	3	1	1	149	1	121	2.0e-56	228.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04915	MS.gene21434	-0.840741	7.24E-58	-1.69E-46
MS.gene04917	MS.gene21434	-0.831522	1.59E-55	-1.69E-46
MS.gene049758	MS.gene21434	-0.805481	1.31E-49	-1.69E-46
MS.gene050114	MS.gene21434	-0.814677	1.36E-51	-1.69E-46
MS.gene050623	MS.gene21434	0.820708	5.95E-53	-1.69E-46
MS.gene050629	MS.gene21434	0.838686	2.48E-57	-1.69E-46
MS.gene050762	MS.gene21434	-0.839944	1.17E-57	-1.69E-46
MS.gene050763	MS.gene21434	-0.841701	4.05E-58	-1.69E-46
MS.gene051982	MS.gene21434	0.812702	3.71E-51	-1.69E-46
MS.gene052115	MS.gene21434	-0.840001	1.13E-57	-1.69E-46
MS.gene052970	MS.gene21434	0.802395	5.73E-49	-1.69E-46
MS.gene054379	MS.gene21434	-0.827827	1.26E-54	-1.69E-46
MS.gene054930	MS.gene21434	-0.800632	1.32E-48	-1.69E-46
MS.gene055174	MS.gene21434	-0.808806	2.58E-50	-1.69E-46
MS.gene055816	MS.gene21434	0.824451	8.02E-54	-1.69E-46
MS.gene057665	MS.gene21434	-0.807449	5.03E-50	-1.69E-46
MS.gene058182	MS.gene21434	-0.818914	1.53E-52	-1.69E-46
MS.gene058661	MS.gene21434	0.847108	1.42E-59	-1.69E-46
MS.gene060084	MS.gene21434	-0.834279	3.28E-56	-1.69E-46
MS.gene060869	MS.gene21434	-0.837574	4.80E-57	-1.69E-46
MS.gene060870	MS.gene21434	-0.814757	1.31E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21434.t1	MTR_1g086310	79.195	149	3	1	1	149	1	121	1.21e-79	231
MS.gene21434.t1	MTR_4g123760	71.028	107	31	0	40	146	12	118	1.02e-54	168
MS.gene21434.t1	MTR_4g048510	56.604	106	46	0	41	146	12	117	5.11e-41	133
MS.gene21434.t1	MTR_2g023430	57.009	107	46	0	42	148	13	119	5.40e-41	133
MS.gene21434.t1	MTR_4g101090	53.704	108	50	0	41	148	12	119	6.35e-39	128
MS.gene21434.t1	MTR_2g088230	56.977	86	37	0	61	146	20	105	4.13e-32	110
MS.gene21434.t1	MTR_7g096540	74.194	62	16	0	85	146	1	62	5.32e-29	101
MS.gene21434.t1	MTR_4g037225	46.296	108	56	2	42	147	13	120	7.68e-25	92.4

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21434.t1	AT3G06420	72.381	105	29	42	146	15	119	2.08e-54	167
MS.gene21434.t1	AT3G15580	72.642	106	29	41	146	10	115	9.81e-54	165
MS.gene21434.t1	AT4G21980	59.633	109	44	41	149	12	120	3.16e-44	142
MS.gene21434.t1	AT4G21980	59.633	109	44	41	149	27	135	4.58e-44	142
MS.gene21434.t1	AT1G62040	59.434	106	43	41	146	12	117	2.21e-42	137
MS.gene21434.t1	AT4G04620	56.481	108	47	41	148	12	119	3.47e-42	136
MS.gene21434.t1	AT4G04620	56.481	108	47	41	148	12	119	3.47e-42	136
MS.gene21434.t1	AT4G04620	56.481	108	47	41	148	12	119	3.47e-42	136
MS.gene21434.t1	AT2G45170	55.556	108	48	42	149	14	121	2.25e-40	132
MS.gene21434.t1	AT2G45170	55.556	108	48	42	149	14	121	2.25e-40	132
MS.gene21434.t1	AT2G05630	54.717	106	48	41	146	12	117	1.55e-39	129
MS.gene21434.t1	AT4G16520	55.660	106	47	41	146	12	117	4.70e-39	128
MS.gene21434.t1	AT4G16520	55.660	106	47	41	146	12	117	4.70e-39	128
MS.gene21434.t1	AT3G60640	54.206	107	49	42	148	13	119	7.36e-39	128
MS.gene21434.t1	AT2G05630	53.271	107	50	40	146	55	161	2.00e-38	128
MS.gene21434.t1	AT4G16520	55.682	88	39	59	146	30	117	1.67e-30	107
MS.gene21434.t1	AT1G62040	55.422	83	37	41	123	12	94	3.61e-27	97.8

Find 23 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGGATTCGATCGGGGTATT+TGG	0.168599	1.2:-65050025	None:intergenic
ATAAGATAAAATTATTAAAA+AGG	0.280951	1.2:-65049926	None:intergenic
TACCAAACGTAAACTCGTTC+TGG	0.338661	1.2:-65049888	None:intergenic
TCCAGCTGGTAAATTAAGTC+TGG	0.354424	1.2:-65050361	None:intergenic
CAAATGCGATCTGCCTCATT+TGG	0.355422	1.2:+65050156	MS.gene21434:CDS
AAGAATACTTTACCTCAAAC+TGG	0.387404	1.2:+65050405	MS.gene21434:CDS
GGACTTACGGGGATTCGATC+GGG	0.390336	1.2:-65050033	None:intergenic
TATAGCACTGAGAAAACCTT+TGG	0.412532	1.2:+65050585	MS.gene21434:CDS
TGCTATTGCAGCTAGTATGA+TGG	0.424492	1.2:+65050512	MS.gene21434:intron
GCAAGTTTATTACCAGTTTG+AGG	0.425620	1.2:-65050417	None:intergenic
CAAATGAGGCAGATCGCATT+TGG	0.427187	1.2:-65050155	None:intergenic
AATAGTTGACATTTATGTAC+AGG	0.446245	1.2:-65050610	None:intergenic
TCCAGACTTAATTTACCAGC+TGG	0.456823	1.2:+65050360	MS.gene21434:CDS
TCTTCTTCTTCAGGTGATTG+TGG	0.457646	1.2:+65050123	MS.gene21434:intron
CTCTGTTTATAGATCATTCA+AGG	0.464700	1.2:+65050536	MS.gene21434:CDS
TTCCAGAACGAGTTTACGTT+TGG	0.466165	1.2:+65049886	MS.gene21434:CDS
GGGACTTACGGGGATTCGAT+CGG	0.493031	1.2:-65050034	None:intergenic
TTATAGATCATTCAAGGATG+AGG	0.555700	1.2:+65050542	MS.gene21434:CDS
AGATCATTCAAGGATGAGGA+TGG	0.557576	1.2:+65050546	MS.gene21434:CDS
CATTTATGTACAGGATCCAA+AGG	0.566947	1.2:-65050601	None:intergenic
GTTCCACGAGACTTGTCAGT+TGG	0.569472	1.2:+65050315	MS.gene21434:CDS
GACTTACGGGGATTCGATCG+GGG	0.607489	1.2:-65050032	None:intergenic
TGTCCAACTGACAAGTCTCG+TGG	0.654531	1.2:-65050318	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAAAAATAGATTTAAGATT+AGG	-	chr1.2:65050448-65050467	None:intergenic	10.0%
!!	AAGCCAAATTGAAATTAAAT+TGG	-	chr1.2:65050085-65050104	None:intergenic	20.0%
!!!	AATAATTTTTCTTCTTCTTC+AGG	+	chr1.2:65050114-65050133	MS.gene21434:intron	20.0%
!	ATACGAAAATCAATGATTGA+TGG	-	chr1.2:65050058-65050077	None:intergenic	25.0%
!	CCAAATTGAAATTAAATTGG+AGG	-	chr1.2:65050082-65050101	None:intergenic	25.0%
!	TACGAAAATCAATGATTGAT+GGG	-	chr1.2:65050057-65050076	None:intergenic	25.0%
!!	CCTCCAATTTAATTTCAATT+TGG	+	chr1.2:65050079-65050098	MS.gene21434:intron	25.0%
	AAGAATACTTTACCTCAAAC+TGG	+	chr1.2:65050405-65050424	MS.gene21434:CDS	30.0%
	CTCTGTTTATAGATCATTCA+AGG	+	chr1.2:65050536-65050555	MS.gene21434:CDS	30.0%
	TTATAGATCATTCAAGGATG+AGG	+	chr1.2:65050542-65050561	MS.gene21434:CDS	30.0%
!	TTTTCTCAGTGCTATAATAC+AGG	-	chr1.2:65050581-65050600	None:intergenic	30.0%
!!!	TACTTTTTCTTCTCCAAATG+AGG	-	chr1.2:65050172-65050191	None:intergenic	30.0%
	CAACATGAAAAAGCAACAAG+AGG	-	chr1.2:65050479-65050498	None:intergenic	35.0%
	GCAAGTTTATTACCAGTTTG+AGG	-	chr1.2:65050420-65050439	None:intergenic	35.0%
	TCAATGATTGATGGGACTTA+CGG	-	chr1.2:65050049-65050068	None:intergenic	35.0%
!	TATAGCACTGAGAAAACCTT+TGG	+	chr1.2:65050585-65050604	MS.gene21434:CDS	35.0%
	AATGATTGATGGGACTTACG+GGG	-	chr1.2:65050047-65050066	None:intergenic	40.0%
	AGATCATTCAAGGATGAGGA+TGG	+	chr1.2:65050546-65050565	MS.gene21434:CDS	40.0%
	CAATGATTGATGGGACTTAC+GGG	-	chr1.2:65050048-65050067	None:intergenic	40.0%
	TACCAAACGTAAACTCGTTC+TGG	-	chr1.2:65049891-65049910	None:intergenic	40.0%
	TCCAGACTTAATTTACCAGC+TGG	+	chr1.2:65050360-65050379	MS.gene21434:CDS	40.0%
	TCCAGCTGGTAAATTAAGTC+TGG	-	chr1.2:65050364-65050383	None:intergenic	40.0%
	TCTTCTTCTTCAGGTGATTG+TGG	+	chr1.2:65050123-65050142	MS.gene21434:intron	40.0%
	TGCTATTGCAGCTAGTATGA+TGG	+	chr1.2:65050512-65050531	MS.gene21434:intron	40.0%
	TTCCAGAACGAGTTTACGTT+TGG	+	chr1.2:65049886-65049905	MS.gene21434:CDS	40.0%
!	TTTTCGCGTGTGTAGAACAA+AGG	+	chr1.2:65049977-65049996	MS.gene21434:CDS	40.0%
	CAAATGAGGCAGATCGCATT+TGG	-	chr1.2:65050158-65050177	None:intergenic	45.0%
	CAAATGCGATCTGCCTCATT+TGG	+	chr1.2:65050156-65050175	MS.gene21434:CDS	45.0%
!	ACAAAGAGAGCTTTTCCAGC+TGG	-	chr1.2:65050378-65050397	None:intergenic	45.0%
!!	ATAAGATAAAATTATTAAAA+AGG	-	chr1.2:65049929-65049948	None:intergenic	5.0%
	GTTCCACGAGACTTGTCAGT+TGG	+	chr1.2:65050315-65050334	MS.gene21434:CDS	50.0%
	TGTCCAACTGACAAGTCTCG+TGG	-	chr1.2:65050321-65050340	None:intergenic	50.0%
	GACTTACGGGGATTCGATCG+GGG	-	chr1.2:65050035-65050054	None:intergenic	55.0%
	GGACTTACGGGGATTCGATC+GGG	-	chr1.2:65050036-65050055	None:intergenic	55.0%
	GGGACTTACGGGGATTCGAT+CGG	-	chr1.2:65050037-65050056	None:intergenic	55.0%
!	GGGGATTCGATCGGGGTATT+TGG	-	chr1.2:65050028-65050047	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	65049868	65050620	65049868	ID=MS.gene21434
chr1.2	mRNA	65049868	65050620	65049868	ID=MS.gene21434.t1;Parent=MS.gene21434
chr1.2	exon	65049868	65050047	65049868	ID=MS.gene21434.t1.exon1;Parent=MS.gene21434.t1
chr1.2	CDS	65049868	65050047	65049868	ID=cds.MS.gene21434.t1;Parent=MS.gene21434.t1
chr1.2	exon	65050136	65050188	65050136	ID=MS.gene21434.t1.exon2;Parent=MS.gene21434.t1
chr1.2	CDS	65050136	65050188	65050136	ID=cds.MS.gene21434.t1;Parent=MS.gene21434.t1
chr1.2	exon	65050308	65050426	65050308	ID=MS.gene21434.t1.exon3;Parent=MS.gene21434.t1
chr1.2	CDS	65050308	65050426	65050308	ID=cds.MS.gene21434.t1;Parent=MS.gene21434.t1
chr1.2	exon	65050523	65050620	65050523	ID=MS.gene21434.t1.exon4;Parent=MS.gene21434.t1
chr1.2	CDS	65050523	65050620	65050523	ID=cds.MS.gene21434.t1;Parent=MS.gene21434.t1

Gene Sequence

>MS.gene21434

ATGGGAGGAGGTTCTTGTTTCCAGAACGAGTTTACGTTTGGTACATTAGATTCTTCATCCTTTTTAATAATTTTATCTTATTTATTCATCAATTTTTGTAAGAGAATTGTTTTCGCGTGTGTAGAACAAAGGCTTCAAGAATCACGTGATATTATTGCCAAATACCCCGATCGAATCCCCGTAAGTCCCATCAATCATTGATTTTCGTATTCCTCCAATTTAATTTCAATTTGGCTTTCAATTTTGAATAATTTTTCTTCTTCTTCAGGTGATTGTGGAAAAGTATACCAAATGCGATCTGCCTCATTTGGAGAAGAAAAAGTAAGACTATAAATCAAATTTGAGTTAGATAAATAAATAAATGCATAAAACTTTATGCATAAGTTGTAATTATAATAACTTACTTAGTAATTTTATCATTTGTTATATTTTTGTGATAGATATCTTGTTCCACGAGACTTGTCAGTTGGACATTTCATTCATATTTTGAGTTCCAGACTTAATTTACCAGCTGGAAAAGCTCTCTTTGTATTTGTTAAGAATACTTTACCTCAAACTGGTAATAAACTTGCATTATCCTAATCTTAAATCTATTTTTTTTTTTTCTTCCTCTTGTTGCTTTTTCATGTTGCTGTGTTTATTTTTGCTATTGCAGCTAGTATGATGGACTCTGTTTATAGATCATTCAAGGATGAGGATGGATTTCTTTACCTGTATTATAGCACTGAGAAAACCTTTGGATCCTGTACATAA

Protein sequence

>MS.gene21434.t1

MGGGSCFQNEFTFGTLDSSSFLIILSYLFINFCKRIVFACVEQRLQESRDIIAKYPDRIPVIVEKYTKCDLPHLEKKKYLVPRDLSVGHFIHILSSRLNLPAGKALFVFVKNTLPQTASMMDSVYRSFKDEDGFLYLYYSTEKTFGSCT