AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052095

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052095.t1 MTR_2g097390 92.081 644 45 3 1 639 1 643 0.0 1175
MS.gene052095.t1 MTR_2g097310 83.179 648 92 7 1 639 1 640 0.0 1062
MS.gene052095.t1 MTR_2g097350 80.740 649 108 5 1 639 1 642 0.0 1014
MS.gene052095.t1 MTR_2g097380 80.122 493 55 4 147 639 114 563 0.0 806
MS.gene052095.t1 MTR_4g064160 47.406 694 283 16 17 639 4 686 0.0 570
MS.gene052095.t1 MTR_4g064120 42.351 621 315 16 42 639 28 628 1.05e-152 455
MS.gene052095.t1 MTR_2g097410 50.572 437 200 7 216 639 310 743 4.24e-150 452
MS.gene052095.t1 MTR_4g064180 41.546 621 320 15 42 639 28 628 3.85e-148 443
MS.gene052095.t1 MTR_7g062120 50.597 419 195 6 226 639 331 742 3.97e-147 444
MS.gene052095.t1 MTR_4g064150 48.064 439 211 7 216 639 299 735 6.78e-145 438
MS.gene052095.t1 MTR_2g097463 37.881 623 334 15 50 639 55 657 5.39e-139 421
MS.gene052095.t1 MTR_2g097467 38.498 626 333 15 46 639 51 656 2.19e-138 419
MS.gene052095.t1 MTR_4g064200 46.552 464 214 8 200 639 199 652 1.48e-137 417
MS.gene052095.t1 MTR_2g097473 45.287 435 224 6 214 639 227 656 1.05e-131 402
MS.gene052095.t1 MTR_5g097480 28.455 492 315 9 160 636 74 543 5.01e-63 218
MS.gene052095.t1 MTR_3g056110 28.972 535 328 15 127 636 34 541 1.23e-59 209
MS.gene052095.t1 MTR_4g133660 30.079 379 253 5 266 637 170 543 4.06e-59 207
MS.gene052095.t1 MTR_2g026250 29.765 383 258 5 262 636 216 595 6.46e-58 205
MS.gene052095.t1 MTR_2g082090 31.565 377 245 4 267 636 208 578 9.36e-58 204
MS.gene052095.t1 MTR_5g094450 30.789 380 248 8 267 636 157 531 1.28e-54 195
MS.gene052095.t1 MTR_3g089055 29.301 372 256 3 269 636 196 564 1.99e-54 195
MS.gene052095.t1 MTR_3g089055 29.301 372 256 3 269 636 201 569 2.10e-54 195
MS.gene052095.t1 MTR_1g029420 29.570 372 255 3 269 636 223 591 2.32e-53 193
MS.gene052095.t1 MTR_7g027190 30.711 394 234 8 266 636 295 672 4.91e-52 191
MS.gene052095.t1 MTR_3g065980 30.964 394 238 8 261 636 162 539 1.06e-44 167
MS.gene052095.t1 MTR_4g104020 28.010 382 254 8 268 636 146 519 2.14e-44 166
MS.gene052095.t1 MTR_8g442410 27.621 391 247 8 266 636 150 524 5.89e-44 165
MS.gene052095.t1 MTR_4g102790 26.166 386 261 9 267 638 288 663 2.64e-42 162
MS.gene052095.t1 MTR_7g074650 30.411 365 240 8 275 638 442 793 3.00e-42 164
MS.gene052095.t1 MTR_5g009080 24.658 438 264 9 260 636 45 477 9.87e-37 144
MS.gene052095.t1 MTR_3g022830 24.096 415 260 7 272 636 26 435 5.72e-36 140
MS.gene052095.t1 MTR_4g076140 24.485 437 265 9 260 636 37 468 3.03e-35 139
MS.gene052095.t1 MTR_4g077760 28.158 380 246 10 268 636 191 554 7.37e-33 134
MS.gene052095.t1 MTR_4g097080 24.742 388 256 10 271 638 133 504 1.23e-32 132
MS.gene052095.t1 MTR_1g096030 27.157 394 244 13 271 636 80 458 2.83e-30 124
MS.gene052095.t1 MTR_2g089100 26.456 412 248 10 268 639 49 445 3.60e-30 124
MS.gene052095.t1 MTR_2g034260 24.359 390 271 8 263 636 202 583 3.83e-30 125
MS.gene052095.t1 MTR_2g034250 24.485 388 274 6 263 636 202 584 4.84e-30 125
MS.gene052095.t1 MTR_5g015490 25.131 382 268 10 271 638 114 491 1.23e-29 123
MS.gene052095.t1 MTR_1g069725 26.036 507 316 20 143 636 2 462 1.35e-28 120
MS.gene052095.t1 MTR_7g109580 25.263 380 265 9 266 636 184 553 2.62e-28 120
MS.gene052095.t1 MTR_7g057230 26.804 291 194 6 356 636 375 656 9.80e-28 119
MS.gene052095.t1 MTR_2g034280 23.575 386 277 7 263 636 195 574 2.08e-27 117
MS.gene052095.t1 MTR_4g076020 26.738 374 235 12 275 639 88 431 4.51e-27 115
MS.gene052095.t1 MTR_6g047750 24.603 378 260 10 272 636 296 661 4.16e-24 107
MS.gene052095.t1 MTR_5g058860 25.761 427 265 18 237 636 95 496 1.59e-23 105
MS.gene052095.t1 MTR_4g095500 22.642 424 267 10 268 639 56 470 1.59e-21 98.6
MS.gene052095.t1 MTR_2g099110 26.667 390 253 10 271 638 74 452 1.69e-21 98.2
MS.gene052095.t1 MTR_1g086970 24.372 398 253 15 266 636 101 477 2.70e-21 97.8
MS.gene052095.t1 MTR_3g072710 24.505 404 253 16 266 636 115 499 8.78e-21 96.7
MS.gene052095.t1 MTR_5g015950 21.127 426 273 9 268 639 32 448 4.00e-20 94.0
MS.gene052095.t1 MTR_3g053270 21.127 426 273 9 268 639 32 448 4.00e-20 94.0
MS.gene052095.t1 MTR_8g093070 22.500 400 277 14 252 639 120 498 4.21e-17 85.1
MS.gene052095.t1 MTR_0092s0100 24.074 378 251 11 272 636 373 727 8.59e-17 84.7
MS.gene052095.t1 MTR_0092s0100 24.074 378 251 11 272 636 376 730 9.24e-17 84.7
MS.gene052095.t1 MTR_4g026485 23.733 375 255 12 272 636 270 623 3.02e-15 79.7
MS.gene052095.t1 MTR_2g097370 84.375 32 5 0 58 89 1 32 1.62e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052095.t1 AT2G29060 42.788 617 312 12 46 638 57 656 5.64e-165 487
MS.gene052095.t1 AT1G07530 51.299 462 202 8 197 639 310 767 1.38e-160 480
MS.gene052095.t1 AT2G29060 48.690 458 218 6 197 638 237 693 7.34e-157 468
MS.gene052095.t1 AT1G07520 47.982 446 217 8 205 639 90 531 1.85e-140 420
MS.gene052095.t1 AT1G07520 47.982 446 217 8 205 639 90 531 1.85e-140 420
MS.gene052095.t1 AT1G07520 48.506 435 210 7 215 639 265 695 4.57e-139 422
MS.gene052095.t1 AT1G07520 48.506 435 210 7 215 639 266 696 5.01e-139 422
MS.gene052095.t1 AT2G29065 40.625 608 336 13 46 639 34 630 2.88e-137 415
MS.gene052095.t1 AT2G37650 48.158 380 189 4 263 638 327 702 1.70e-123 382
MS.gene052095.t1 AT2G37650 48.158 380 189 4 263 638 339 714 2.25e-123 382
MS.gene052095.t1 AT3G46600 43.294 425 231 5 219 639 131 549 5.74e-118 363
MS.gene052095.t1 AT3G46600 43.294 425 231 5 219 639 163 581 1.28e-117 363
MS.gene052095.t1 AT5G59450 40.044 457 260 6 195 639 146 600 4.46e-115 357
MS.gene052095.t1 AT3G46600 45.213 376 199 4 268 639 1 373 1.35e-112 343
MS.gene052095.t1 AT5G48150 33.067 375 243 5 267 636 118 489 3.09e-65 223
MS.gene052095.t1 AT5G48150 33.067 375 243 5 267 636 118 489 3.09e-65 223
MS.gene052095.t1 AT5G48150 33.067 375 243 5 267 636 118 489 3.09e-65 223
MS.gene052095.t1 AT5G48150 33.067 375 243 5 267 636 118 489 3.09e-65 223
MS.gene052095.t1 AT2G04890 31.551 374 242 4 267 636 49 412 2.64e-64 218
MS.gene052095.t1 AT1G50600 31.078 399 260 5 245 636 135 525 9.93e-61 211
MS.gene052095.t1 AT1G50600 31.078 399 260 5 245 636 206 596 2.63e-60 212
MS.gene052095.t1 AT1G50600 31.078 399 260 5 245 636 210 600 3.08e-60 212
MS.gene052095.t1 AT1G21450 28.008 532 353 15 122 636 74 592 4.87e-55 197
MS.gene052095.t1 AT4G17230 28.912 377 261 3 266 638 153 526 2.51e-49 180
MS.gene052095.t1 AT4G17230 28.912 377 261 3 266 638 153 526 2.51e-49 180
MS.gene052095.t1 AT1G66350 31.070 383 235 10 261 636 145 505 1.58e-44 166
MS.gene052095.t1 AT2G01570 30.829 386 238 11 261 636 214 580 3.30e-44 167
MS.gene052095.t1 AT3G54220 31.169 385 248 9 258 638 280 651 9.23e-44 167
MS.gene052095.t1 AT3G03450 28.238 386 253 8 261 636 173 544 4.74e-43 163
MS.gene052095.t1 AT1G14920 29.058 382 250 8 261 636 162 528 8.49e-43 162
MS.gene052095.t1 AT5G17490 26.984 378 258 7 263 636 152 515 6.19e-41 157
MS.gene052095.t1 AT5G66770 29.867 375 235 12 275 636 224 583 6.38e-34 137
MS.gene052095.t1 AT1G50420 22.664 428 263 9 271 636 57 478 1.09e-33 135
MS.gene052095.t1 AT1G63100 27.684 354 235 9 267 611 248 589 6.19e-32 131
MS.gene052095.t1 AT1G63100 27.684 354 235 9 267 611 273 614 6.88e-32 132
MS.gene052095.t1 AT5G41920 29.275 386 236 14 265 636 37 399 1.11e-30 125
MS.gene052095.t1 AT4G37650 25.000 360 236 9 270 604 145 495 2.54e-29 122
MS.gene052095.t1 AT3G50650 26.171 363 235 10 291 636 195 541 1.92e-28 120
MS.gene052095.t1 AT5G52510 24.289 387 261 9 269 636 266 639 4.06e-27 117
MS.gene052095.t1 AT1G55580 25.604 414 253 11 268 636 41 444 1.65e-22 101
MS.gene052095.t1 AT3G49950 23.846 390 271 9 268 638 27 409 4.22e-22 99.8
MS.gene052095.t1 AT4G08250 24.041 391 269 12 263 636 97 476 5.65e-22 100
MS.gene052095.t1 AT3G60630 28.571 210 125 8 342 537 327 525 1.72e-11 67.8

Find 104 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGAAAATGGAGGAGTATT+TGG 0.132396 2.3:+5594993 None:intergenic
TTCACTCTCTCCAATGAATT+TGG 0.151096 2.3:+5594231 None:intergenic
AGTCTTCACATTATAGATTT+TGG 0.160984 2.3:-5594348 MS.gene052095:CDS
TCTTAATCTGGTTTAATAAT+TGG 0.162293 2.3:+5594607 None:intergenic
ATGCTATGAATAAATATATT+TGG 0.170780 2.3:+5593982 None:intergenic
GAATTCTCTTCCACTGTTCT+TGG 0.211285 2.3:-5595437 MS.gene052095:CDS
GATTGAGAAACAAAGTTATT+AGG 0.220917 2.3:+5594972 None:intergenic
ACAAAGTTATTAGGAGAAAA+TGG 0.226727 2.3:+5594981 None:intergenic
TTTCTCAATGTGTCACAAAA+TGG 0.237333 2.3:-5594870 MS.gene052095:CDS
AGTCTTCTTCCATTAGAAAT+TGG 0.248268 2.3:+5595309 None:intergenic
TTTATTCATAGCATTGTTAA+TGG 0.248656 2.3:-5593973 MS.gene052095:CDS
GTGAAGACTTTCTGTTTCTT+TGG 0.249798 2.3:+5594362 None:intergenic
TTCTATTTGGTTCTTCTTGT+TGG 0.254378 2.3:-5593585 MS.gene052095:CDS
TAGAAGAATGTTTCTTAATC+TGG 0.256983 2.3:+5594595 None:intergenic
CAAACAGATTCTATCGTTCT+AGG 0.263554 2.3:-5594719 MS.gene052095:CDS
CATATATGCAATGGCATTCT+AGG 0.264227 2.3:-5593753 MS.gene052095:CDS
TTTGATAAAGTACTAGTTCT+TGG 0.268048 2.3:-5594801 MS.gene052095:CDS
TGAAATATCATTACAAGAAA+TGG 0.276552 2.3:+5594638 None:intergenic
ATTATTGAAAGAAGGGAAAA+AGG 0.276813 2.3:+5594941 None:intergenic
AAACTATAATCACTGAAGTT+AGG 0.291953 2.3:+5595095 None:intergenic
GAATCCGAGAGGTGCTGTTT+TGG 0.303272 2.3:-5594025 MS.gene052095:CDS
GAGAAAATGGAGGAGTATTT+GGG 0.303863 2.3:+5594994 None:intergenic
TGTTTGAGGTGAAGGAAAAT+TGG 0.305166 2.3:-5593633 MS.gene052095:CDS
TTCTATAAACCAATTTCTAA+TGG 0.312579 2.3:-5595318 MS.gene052095:CDS
TGGAAGGGTAGAATTCTATT+TGG 0.313548 2.3:-5593598 MS.gene052095:CDS
CATTCTAGGCACATGGGAAA+TGG 0.344559 2.3:-5593739 MS.gene052095:CDS
GATAGATCTTCCAAATTCAT+TGG 0.350686 2.3:-5594241 MS.gene052095:CDS
TTGGGAAAGTATCAAAGTTC+AGG 0.363131 2.3:-5594133 MS.gene052095:CDS
TGGGTTTAGATCTTTGAAAC+TGG 0.374406 2.3:-5593719 MS.gene052095:CDS
TTGATGATTCTTCATTCTGC+TGG 0.378672 2.3:+5594739 None:intergenic
TCCTCAAACATCAACCTAAC+AGG 0.379387 2.3:+5593847 None:intergenic
GGAGTATTTGGGAGTAGAAA+AGG 0.380526 2.3:+5595005 None:intergenic
ACCTGATGAGACAGTTGTTT+CGG 0.393796 2.3:-5594049 MS.gene052095:CDS
TAGCCTGTGAAGGCTGTGAT+AGG 0.399278 2.3:-5593792 MS.gene052095:CDS
CTTGGTACTAAATTCACAAA+AGG 0.404219 2.3:-5594783 MS.gene052095:CDS
CTTGAAGAGCAAAAGAATCT+TGG 0.409666 2.3:+5595264 None:intergenic
GATGTTTGAGGAAGACGTTT+GGG 0.411150 2.3:-5593836 MS.gene052095:CDS
GCAATACCATTGCACTCAAA+AGG 0.411689 2.3:+5594162 None:intergenic
AAGAAACATTCTTCTACAAC+AGG 0.417986 2.3:-5594588 MS.gene052095:CDS
GAGTGAATGAAACAGGTCTT+AGG 0.420195 2.3:-5594215 MS.gene052095:CDS
GAAAGAGCATTATTGAAAGA+AGG 0.427388 2.3:+5594933 None:intergenic
GAGTATTTGGGAGTAGAAAA+GGG 0.432472 2.3:+5595006 None:intergenic
TTGGAGAGAGTGAATGAAAC+AGG 0.438708 2.3:-5594222 MS.gene052095:CDS
ATGAGGAAGTTGTGGATTTG+AGG 0.441434 2.3:-5594686 MS.gene052095:CDS
AACTATAATCACTGAAGTTA+GGG 0.442010 2.3:+5595096 None:intergenic
AAAGAGCATTATTGAAAGAA+GGG 0.443472 2.3:+5594934 None:intergenic
GAAGCACGTTTAGCAGGAAC+TGG 0.446336 2.3:-5594522 MS.gene052095:CDS
TGATGTTTGAGGAAGACGTT+TGG 0.448987 2.3:-5593837 MS.gene052095:CDS
ATTCTAGGCACATGGGAAAT+GGG 0.456200 2.3:-5593738 MS.gene052095:CDS
GGTTATGGTTTCAAGTGGCT+TGG 0.456727 2.3:-5594321 MS.gene052095:CDS
TATCCCTGTGATCCTCAACT+TGG 0.460979 2.3:+5594260 None:intergenic
GCAGATTATAAATAAATTGA+AGG 0.461295 2.3:-5593692 MS.gene052095:CDS
GTAACAGCAGCAGCAATAGT+AGG 0.465452 2.3:+5595191 None:intergenic
TCCTGTTAGGTTGATGTTTG+AGG 0.467599 2.3:-5593848 MS.gene052095:CDS
ACTCTTCATGTTCATTGTGT+TGG 0.472559 2.3:+5595357 None:intergenic
TGCCATTGCATATATGTCTC+TGG 0.473005 2.3:+5593760 None:intergenic
GCGCTTGAAGCACGTTTAGC+AGG 0.476214 2.3:-5594528 MS.gene052095:CDS
GTTTATAGAATCAAGAGCAT+TGG 0.477886 2.3:+5595331 None:intergenic
TCCGAAACAACTGTCTCATC+AGG 0.480396 2.3:+5594048 None:intergenic
CCACCCAAGTTGAGGATCAC+AGG 0.486139 2.3:-5594264 MS.gene052095:CDS
TATCGTTTATCGAAAAGATC+TGG 0.498438 2.3:-5594291 MS.gene052095:CDS
TTCTAGGAAACGAAGTTATG+AGG 0.503468 2.3:-5594703 MS.gene052095:CDS
TTGAATGTGAGGATCCTGTT+AGG 0.507367 2.3:-5593861 MS.gene052095:CDS
AAAATTGGATGCTACAAGGT+TGG 0.509330 2.3:-5593618 MS.gene052095:CDS
TTGGTACTAAATTCACAAAA+GGG 0.510419 2.3:-5594782 MS.gene052095:CDS
AACCCTATCACAGCCTTCAC+AGG 0.513850 2.3:+5593789 None:intergenic
GCAATAGTAGGAGGAGGAAG+AGG 0.518270 2.3:+5595203 None:intergenic
CTAGTTGTGAAGGTCTATGC+TGG 0.523269 2.3:+5593559 None:intergenic
CAATAATGCTCTTTCTCATG+AGG 0.525022 2.3:-5594925 MS.gene052095:CDS
ACGAAGTTATGAGGAAGTTG+TGG 0.528730 2.3:-5594694 MS.gene052095:CDS
CATTCTTCTACAACAGGTGA+TGG 0.532486 2.3:-5594582 MS.gene052095:CDS
GGCATGCTGATGAATAAACT+TGG 0.547453 2.3:+5594436 None:intergenic
TTGGATGCTACAAGGTTGGA+AGG 0.548278 2.3:-5593614 MS.gene052095:CDS
CAGATTATAAATAAATTGAA+GGG 0.550775 2.3:-5593691 MS.gene052095:CDS
ATTCATAGCATTGTTAATGG+TGG 0.554304 2.3:-5593970 MS.gene052095:CDS
ATCCCTGTGATCCTCAACTT+GGG 0.558025 2.3:+5594261 None:intergenic
AGCCTGTGAAGGCTGTGATA+GGG 0.569110 2.3:-5593791 MS.gene052095:CDS
GCAATGGCATTCTAGGCACA+TGG 0.578087 2.3:-5593746 MS.gene052095:CDS
ATGTTTGAGGAAGACGTTTG+GGG 0.578234 2.3:-5593835 MS.gene052095:CDS
AAATCCAAAACAGCACCTCT+CGG 0.585044 2.3:+5594021 None:intergenic
GCAGCAGCAATAGTAGGAGG+AGG 0.588588 2.3:+5595197 None:intergenic
TATATGTGGATCAAGTTCAA+AGG 0.594302 2.3:+5595052 None:intergenic
GGCCAGAGACATATATGCAA+TGG 0.597570 2.3:-5593762 MS.gene052095:CDS
CACCCAAGTTGAGGATCACA+GGG 0.601694 2.3:-5594263 MS.gene052095:CDS
CGTTTATCGAAAAGATCTGG+TGG 0.602238 2.3:-5594288 MS.gene052095:CDS
AAGGAAAATTGGATGCTACA+AGG 0.606455 2.3:-5593622 MS.gene052095:CDS
CCTGTGATCCTCAACTTGGG+TGG 0.609546 2.3:+5594264 None:intergenic
GTGTTGGTTATGGTTTCAAG+TGG 0.612035 2.3:-5594326 MS.gene052095:CDS
AGTGATTATAGTTTAAGCAG+TGG 0.619865 2.3:-5595086 MS.gene052095:CDS
TGTTTGAGGAAGACGTTTGG+GGG 0.623857 2.3:-5593834 MS.gene052095:CDS
TAGTTGTATGAGTCTATATG+TGG 0.625308 2.3:+5595038 None:intergenic
ACAGCAGCAGCAATAGTAGG+AGG 0.628822 2.3:+5595194 None:intergenic
TTGTTGATACTCATGCTGGA+TGG 0.638387 2.3:+5595139 None:intergenic
CAATGGCATTCTAGGCACAT+GGG 0.638518 2.3:-5593745 MS.gene052095:CDS
TGGATGCTACAAGGTTGGAA+GGG 0.641743 2.3:-5593613 MS.gene052095:CDS
GTATGCTTTAACCATATCTG+CGG 0.649034 2.3:+5594458 None:intergenic
GTTTAGAAAACCAAGAACAG+TGG 0.650129 2.3:+5595427 None:intergenic
AAGGCTGTGATAGGGTTCAG+AGG 0.656517 2.3:-5593783 MS.gene052095:CDS
CTGGTGGGCCACCCAAGTTG+AGG 0.658509 2.3:-5594272 MS.gene052095:CDS
ATGAATGTTATAGCCTGTGA+AGG 0.667099 2.3:-5593802 MS.gene052095:CDS
TAACAACAATGTTGAATGTG+AGG 0.669855 2.3:-5593872 MS.gene052095:CDS
AAGTTATTAGGAGAAAATGG+AGG 0.670375 2.3:+5594984 None:intergenic
GTTTATCGAAAAGATCTGGT+GGG 0.689733 2.3:-5594287 MS.gene052095:CDS
GTTGTTTCGGAGAATCCGAG+AGG 0.745211 2.3:-5594036 MS.gene052095:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGCTATGAATAAATATATT+TGG + chr2.3:5595064-5595083 None:intergenic 15.0%
!! CAGATTATAAATAAATTGAA+GGG - chr2.3:5595352-5595371 MS.gene052095:CDS 15.0%
!! GCAGATTATAAATAAATTGA+AGG - chr2.3:5595351-5595370 MS.gene052095:CDS 20.0%
!! TGAAATATCATTACAAGAAA+TGG + chr2.3:5594408-5594427 None:intergenic 20.0%
!! TTCTATAAACCAATTTCTAA+TGG - chr2.3:5593725-5593744 MS.gene052095:CDS 20.0%
!! TTTATTCATAGCATTGTTAA+TGG - chr2.3:5595070-5595089 MS.gene052095:CDS 20.0%
!!! ATAATTGCAAGTTTTTCAAA+AGG + chr2.3:5594632-5594651 None:intergenic 20.0%
!!! TAATTGCAAGTTTTTCAAAA+GGG + chr2.3:5594631-5594650 None:intergenic 20.0%
!!! TCTTAATCTGGTTTAATAAT+TGG + chr2.3:5594439-5594458 None:intergenic 20.0%
!!! TTTTCTAAACAAATTTCTCA+TGG - chr2.3:5593629-5593648 MS.gene052095:CDS 20.0%
! AAACTATAATCACTGAAGTT+AGG + chr2.3:5593951-5593970 None:intergenic 25.0%
! AACTATAATCACTGAAGTTA+GGG + chr2.3:5593950-5593969 None:intergenic 25.0%
! ACAAAGTTATTAGGAGAAAA+TGG + chr2.3:5594065-5594084 None:intergenic 25.0%
! ATTATTGAAAGAAGGGAAAA+AGG + chr2.3:5594105-5594124 None:intergenic 25.0%
! CAATGGTATTGCAAAAAATT+GGG - chr2.3:5594892-5594911 MS.gene052095:CDS 25.0%
! GATTGAGAAACAAAGTTATT+AGG + chr2.3:5594074-5594093 None:intergenic 25.0%
! TAGAAGAATGTTTCTTAATC+TGG + chr2.3:5594451-5594470 None:intergenic 25.0%
! TTAAACCTTGTCACAAAAAA+AGG + chr2.3:5595109-5595128 None:intergenic 25.0%
! TTGGTACTAAATTCACAAAA+GGG - chr2.3:5594261-5594280 MS.gene052095:CDS 25.0%
!! AGTCTTCACATTATAGATTT+TGG - chr2.3:5594695-5594714 MS.gene052095:CDS 25.0%
!! TAATAGTGATTTTCTGTTTG+AGG - chr2.3:5595396-5595415 MS.gene052095:CDS 25.0%
!! TTTGATAAAGTACTAGTTCT+TGG - chr2.3:5594242-5594261 MS.gene052095:CDS 25.0%
!!! AAAGAGCATTATTGAAAGAA+GGG + chr2.3:5594112-5594131 None:intergenic 25.0%
!!! AAGTTCAGGATTTTAAGATT+AGG - chr2.3:5594924-5594943 MS.gene052095:CDS 25.0%
!!! TGTTTTGGATTTGATTAAGA+AGG - chr2.3:5595033-5595052 MS.gene052095:CDS 25.0%
!!! TTTTTTTGTGACAAGGTTTA+AGG - chr2.3:5595108-5595127 MS.gene052095:CDS 25.0%
AAGAAACATTCTTCTACAAC+AGG - chr2.3:5594455-5594474 MS.gene052095:CDS 30.0%
AAGTTATTAGGAGAAAATGG+AGG + chr2.3:5594062-5594081 None:intergenic 30.0%
AGTCTTCTTCCATTAGAAAT+TGG + chr2.3:5593737-5593756 None:intergenic 30.0%
AGTGATTATAGTTTAAGCAG+TGG - chr2.3:5593957-5593976 MS.gene052095:CDS 30.0%
ATTCATAGCATTGTTAATGG+TGG - chr2.3:5595073-5595092 MS.gene052095:CDS 30.0%
CAAAAATTGGCACATTTCTT+TGG - chr2.3:5594488-5594507 MS.gene052095:CDS 30.0%
CTTGGTACTAAATTCACAAA+AGG - chr2.3:5594260-5594279 MS.gene052095:CDS 30.0%
GATAGATCTTCCAAATTCAT+TGG - chr2.3:5594802-5594821 MS.gene052095:CDS 30.0%
GCAATGGTATTGCAAAAAAT+TGG - chr2.3:5594891-5594910 MS.gene052095:CDS 30.0%
TAACAACAATGTTGAATGTG+AGG - chr2.3:5595171-5595190 MS.gene052095:CDS 30.0%
TAGTTGTATGAGTCTATATG+TGG + chr2.3:5594008-5594027 None:intergenic 30.0%
TATATGTGGATCAAGTTCAA+AGG + chr2.3:5593994-5594013 None:intergenic 30.0%
TATCGTTTATCGAAAAGATC+TGG - chr2.3:5594752-5594771 MS.gene052095:CDS 30.0%
TTTCTCAATGTGTCACAAAA+TGG - chr2.3:5594173-5594192 MS.gene052095:CDS 30.0%
! GTTTATAGAATCAAGAGCAT+TGG + chr2.3:5593715-5593734 None:intergenic 30.0%
!! CACATTATAGATTTTGGTGT+TGG - chr2.3:5594701-5594720 MS.gene052095:CDS 30.0%
!! GAAAGAGCATTATTGAAAGA+AGG + chr2.3:5594113-5594132 None:intergenic 30.0%
!! TTCTATTTGGTTCTTCTTGT+TGG - chr2.3:5595458-5595477 MS.gene052095:CDS 30.0%
!!! ATAGATTTTGGTGTTGGTTA+TGG - chr2.3:5594707-5594726 MS.gene052095:CDS 30.0%
AAAATTGGATGCTACAAGGT+TGG - chr2.3:5595425-5595444 MS.gene052095:CDS 35.0%
AAGGAAAATTGGATGCTACA+AGG - chr2.3:5595421-5595440 MS.gene052095:CDS 35.0%
ACTCTTCATGTTCATTGTGT+TGG + chr2.3:5593689-5593708 None:intergenic 35.0%
ATGAATGTTATAGCCTGTGA+AGG - chr2.3:5595241-5595260 MS.gene052095:CDS 35.0%
CAAACAGATTCTATCGTTCT+AGG - chr2.3:5594324-5594343 MS.gene052095:CDS 35.0%
CAATAATGCTCTTTCTCATG+AGG - chr2.3:5594118-5594137 MS.gene052095:CDS 35.0%
CATATATGCAATGGCATTCT+AGG - chr2.3:5595290-5595309 MS.gene052095:CDS 35.0%
CTTGAAGAGCAAAAGAATCT+TGG + chr2.3:5593782-5593801 None:intergenic 35.0%
GAGTATTTGGGAGTAGAAAA+GGG + chr2.3:5594040-5594059 None:intergenic 35.0%
GTATGCTTTAACCATATCTG+CGG + chr2.3:5594588-5594607 None:intergenic 35.0%
GTTTAGAAAACCAAGAACAG+TGG + chr2.3:5593619-5593638 None:intergenic 35.0%
GTTTATCGAAAAGATCTGGT+GGG - chr2.3:5594756-5594775 MS.gene052095:CDS 35.0%
TGGAAGGGTAGAATTCTATT+TGG - chr2.3:5595445-5595464 MS.gene052095:CDS 35.0%
TTCACTCTCTCCAATGAATT+TGG + chr2.3:5594815-5594834 None:intergenic 35.0%
! GTGAAGACTTTCTGTTTCTT+TGG + chr2.3:5594684-5594703 None:intergenic 35.0%
! TGTTTGAGGTGAAGGAAAAT+TGG - chr2.3:5595410-5595429 MS.gene052095:CDS 35.0%
! TTCTAGGAAACGAAGTTATG+AGG - chr2.3:5594340-5594359 MS.gene052095:CDS 35.0%
! TTGATGATTCTTCATTCTGC+TGG + chr2.3:5594307-5594326 None:intergenic 35.0%
!! AATGTTCCTTTTGAGTGCAA+TGG - chr2.3:5594875-5594894 MS.gene052095:CDS 35.0%
!! AGGTGATGGAACTCAAAAAT+TGG - chr2.3:5594475-5594494 MS.gene052095:CDS 35.0%
!! GAGAAAATGGAGGAGTATTT+GGG + chr2.3:5594052-5594071 None:intergenic 35.0%
!! TGATTTTCTGTTTGAGGTGA+AGG - chr2.3:5595402-5595421 MS.gene052095:CDS 35.0%
!! TGGGTTTAGATCTTTGAAAC+TGG - chr2.3:5595324-5595343 MS.gene052095:CDS 35.0%
!! TTGGGAAAGTATCAAAGTTC+AGG - chr2.3:5594910-5594929 MS.gene052095:CDS 35.0%
!!! AGTTTTGTTGATACTCATGC+TGG + chr2.3:5593911-5593930 None:intergenic 35.0%
!!! ATGCACCTTTTTTTGTGACA+AGG - chr2.3:5595101-5595120 MS.gene052095:CDS 35.0%
AAATCCAAAACAGCACCTCT+CGG + chr2.3:5595025-5595044 None:intergenic 40.0%
ACCTGATGAGACAGTTGTTT+CGG - chr2.3:5594994-5595013 MS.gene052095:CDS 40.0%
ACGAAGTTATGAGGAAGTTG+TGG - chr2.3:5594349-5594368 MS.gene052095:CDS 40.0%
ATGAGGAAGTTGTGGATTTG+AGG - chr2.3:5594357-5594376 MS.gene052095:CDS 40.0%
ATGTTTGAGGAAGACGTTTG+GGG - chr2.3:5595208-5595227 MS.gene052095:CDS 40.0%
ATTCTAGGCACATGGGAAAT+GGG - chr2.3:5595305-5595324 MS.gene052095:CDS 40.0%
CATTCTTCTACAACAGGTGA+TGG - chr2.3:5594461-5594480 MS.gene052095:CDS 40.0%
CGTTTATCGAAAAGATCTGG+TGG - chr2.3:5594755-5594774 MS.gene052095:CDS 40.0%
GAAAAAATCTGCCGCAGATA+TGG - chr2.3:5594574-5594593 MS.gene052095:CDS 40.0%
GAATTCTCTTCCACTGTTCT+TGG - chr2.3:5593606-5593625 MS.gene052095:CDS 40.0%
GAGTGAATGAAACAGGTCTT+AGG - chr2.3:5594828-5594847 MS.gene052095:CDS 40.0%
GATGTTTGAGGAAGACGTTT+GGG - chr2.3:5595207-5595226 MS.gene052095:CDS 40.0%
GCAATACCATTGCACTCAAA+AGG + chr2.3:5594884-5594903 None:intergenic 40.0%
GGCATGCTGATGAATAAACT+TGG + chr2.3:5594610-5594629 None:intergenic 40.0%
TCATGCTGGATGGAAAAAGA+AGG + chr2.3:5593897-5593916 None:intergenic 40.0%
TCCTCAAACATCAACCTAAC+AGG + chr2.3:5595199-5595218 None:intergenic 40.0%
TGATGTTTGAGGAAGACGTT+TGG - chr2.3:5595206-5595225 MS.gene052095:CDS 40.0%
TGCCATTGCATATATGTCTC+TGG + chr2.3:5595286-5595305 None:intergenic 40.0%
TTGAATGTGAGGATCCTGTT+AGG - chr2.3:5595182-5595201 MS.gene052095:CDS 40.0%
TTGGAGAGAGTGAATGAAAC+AGG - chr2.3:5594821-5594840 MS.gene052095:CDS 40.0%
TTGTTGATACTCATGCTGGA+TGG + chr2.3:5593907-5593926 None:intergenic 40.0%
! GGAGTATTTGGGAGTAGAAA+AGG + chr2.3:5594041-5594060 None:intergenic 40.0%
!! GGAGAAAATGGAGGAGTATT+TGG + chr2.3:5594053-5594072 None:intergenic 40.0%
!! GTGTTGGTTATGGTTTCAAG+TGG - chr2.3:5594717-5594736 MS.gene052095:CDS 40.0%
!! TCCTGTTAGGTTGATGTTTG+AGG - chr2.3:5595195-5595214 MS.gene052095:CDS 40.0%
!!! ATCTGCGGCAGATTTTTTCT+TGG + chr2.3:5594573-5594592 None:intergenic 40.0%
!!! TGGAAGCTTGTGTTTTGAAG+TGG + chr2.3:5594287-5594306 None:intergenic 40.0%
ATCCCTGTGATCCTCAACTT+GGG + chr2.3:5594785-5594804 None:intergenic 45.0%
CAATGGCATTCTAGGCACAT+GGG - chr2.3:5595298-5595317 MS.gene052095:CDS 45.0%
CATTCTAGGCACATGGGAAA+TGG - chr2.3:5595304-5595323 MS.gene052095:CDS 45.0%
GGCCAGAGACATATATGCAA+TGG - chr2.3:5595281-5595300 MS.gene052095:CDS 45.0%
GTAACAGCAGCAGCAATAGT+AGG + chr2.3:5593855-5593874 None:intergenic 45.0%
TATCCCTGTGATCCTCAACT+TGG + chr2.3:5594786-5594805 None:intergenic 45.0%
TCCGAAACAACTGTCTCATC+AGG + chr2.3:5594998-5595017 None:intergenic 45.0%
TGGATGCTACAAGGTTGGAA+GGG - chr2.3:5595430-5595449 MS.gene052095:CDS 45.0%
TGTTTGAGGAAGACGTTTGG+GGG - chr2.3:5595209-5595228 MS.gene052095:CDS 45.0%
TTGGATGCTACAAGGTTGGA+AGG - chr2.3:5595429-5595448 MS.gene052095:CDS 45.0%
! GGTTATGGTTTCAAGTGGCT+TGG - chr2.3:5594722-5594741 MS.gene052095:CDS 45.0%
AACCCTATCACAGCCTTCAC+AGG + chr2.3:5595257-5595276 None:intergenic 50.0%
AAGGCTGTGATAGGGTTCAG+AGG - chr2.3:5595260-5595279 MS.gene052095:CDS 50.0%
ACAGCAGCAGCAATAGTAGG+AGG + chr2.3:5593852-5593871 None:intergenic 50.0%
AGCCTGTGAAGGCTGTGATA+GGG - chr2.3:5595252-5595271 MS.gene052095:CDS 50.0%
GAAGCACGTTTAGCAGGAAC+TGG - chr2.3:5594521-5594540 MS.gene052095:CDS 50.0%
GCAATAGTAGGAGGAGGAAG+AGG + chr2.3:5593843-5593862 None:intergenic 50.0%
GCAATGGCATTCTAGGCACA+TGG - chr2.3:5595297-5595316 MS.gene052095:CDS 50.0%
TAGCCTGTGAAGGCTGTGAT+AGG - chr2.3:5595251-5595270 MS.gene052095:CDS 50.0%
! CACCCAAGTTGAGGATCACA+GGG - chr2.3:5594780-5594799 MS.gene052095:CDS 50.0%
! GTTGTTTCGGAGAATCCGAG+AGG - chr2.3:5595007-5595026 MS.gene052095:CDS 50.0%
!!! GAATCCGAGAGGTGCTGTTT+TGG - chr2.3:5595018-5595037 MS.gene052095:CDS 50.0%
CCTGTGATCCTCAACTTGGG+TGG + chr2.3:5594782-5594801 None:intergenic 55.0%
GCAGCAGCAATAGTAGGAGG+AGG + chr2.3:5593849-5593868 None:intergenic 55.0%
GCGCTTGAAGCACGTTTAGC+AGG - chr2.3:5594515-5594534 MS.gene052095:CDS 55.0%
! CCACCCAAGTTGAGGATCAC+AGG - chr2.3:5594779-5594798 MS.gene052095:CDS 55.0%
! CTGGTGGGCCACCCAAGTTG+AGG - chr2.3:5594771-5594790 MS.gene052095:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 5593573 5595492 5593573 ID=MS.gene052095
chr2.3 mRNA 5593573 5595492 5593573 ID=MS.gene052095.t1;Parent=MS.gene052095
chr2.3 exon 5593573 5595492 5593573 ID=MS.gene052095.t1.exon1;Parent=MS.gene052095.t1
chr2.3 CDS 5593573 5595492 5593573 ID=cds.MS.gene052095.t1;Parent=MS.gene052095.t1
Gene Sequence

>MS.gene052095

ATGGAGAAAAGAGTTGTCGATCAAAGTAAGAGTGAATTCTCTTCCACTGTTCTTGGTTTTCTAAACAAATTTCTCATGGAAGATGATGACATAGAAGAAGACTACAATATGTTCCAACACAATGAACATGAAGAGTCATCCAATGCTCTTGATTCTATAAACCAATTTCTAATGGAAGAAGACTTTGAAAAAAAGTACACTATGTTCCAAGATTCTTTTGCTCTTCAAGTTACTGAAAAATCATTTCATGATGCTATTGCTCACACTCCTCTTCCTCCTCCTACTATTGCTGCTGCTGTTACTGCTATTACTACTACTCTTCCTTCTTTTTCCATCCAGCATGAGTATCAACAAAACTACAACTTTGTGCATAGCCCTAACTTCAGTGATTATAGTTTAAGCAGTGGTAGCACTAGCTCCTTTGAACTTGATCCACATATAGACTCATACAACTACAATTATAACCCTTTTCTACTCCCAAATACTCCTCCATTTTCTCCTAATAACTTTGTTTCTCAATCTAATTCAACCTTTTTCCCTTCTTTCAATAATGCTCTTTCTCATGAGGTACTTCAGACAGAAAACTTTGAAAAAGAGCATTTTCTCAATGTGTCACAAAATGGATCAGAACAAGTGTATGTAGATGATAGCAGTGAGTTATCAGAATTATTTGATAAAGTACTAGTTCTTGGTACTAAATTCACAAAAGGGCCACTTCAAAACACAAGCTTCCAGCAGAATGAAGAATCATCAAACAGATTCTATCGTTCTAGGAAACGAAGTTATGAGGAAGTTGTGGATTTGAGGACACTATTGATGCTGTGTGCACAATCCATTTCTTGTAATGATATTTCAAATGCTAACCAATTATTAAACCAGATTAAGAAACATTCTTCTACAACAGGTGATGGAACTCAAAAATTGGCACATTTCTTTGGAAATGCGCTTGAAGCACGTTTAGCAGGAACTGGTTCACATATCTATAGAGCTCTATCTTCCAAGAAAAAATCTGCCGCAGATATGGTTAAAGCATACCAAGTTTATTCATCAGCATGCCCTTTTGAAAAACTTGCAATTATGTTCTCAAACGATGCAATTTTGAATGTAACCAAAGAAACAGAAAGTCTTCACATTATAGATTTTGGTGTTGGTTATGGTTTCAAGTGGCTTGGTTTTATATATCGTTTATCGAAAAGATCTGGTGGGCCACCCAAGTTGAGGATCACAGGGATAGATCTTCCAAATTCATTGGAGAGAGTGAATGAAACAGGTCTTAGGCTTGCAAGTTATTGCAAACGTTTCAATGTTCCTTTTGAGTGCAATGGTATTGCAAAAAATTGGGAAAGTATCAAAGTTCAGGATTTTAAGATTAGGAAAAATGAGTTTGTTGCTGTGACTTGTGTGTTTAAGTTTGAGAATCTACCTGATGAGACAGTTGTTTCGGAGAATCCGAGAGGTGCTGTTTTGGATTTGATTAAGAAGGCAAATCCAAATATATTTATTCATAGCATTGTTAATGGTGGTTATGATGCACCTTTTTTTGTGACAAGGTTTAAGGAAGCTGTTTTTTATTATTCAGCATTGTTTGATATGCTTGATAACAACAATGTTGAATGTGAGGATCCTGTTAGGTTGATGTTTGAGGAAGACGTTTGGGGGAAAGATATAATGAATGTTATAGCCTGTGAAGGCTGTGATAGGGTTCAGAGGCCAGAGACATATATGCAATGGCATTCTAGGCACATGGGAAATGGGTTTAGATCTTTGAAACTGGATAAGCAGATTATAAATAAATTGAAGGGTAAGTTAAGAGATGATGCATATAATAGTGATTTTCTGTTTGAGGTGAAGGAAAATTGGATGCTACAAGGTTGGAAGGGTAGAATTCTATTTGGTTCTTCTTGTTGGATTCCAGCATAG
Protein sequence

>MS.gene052095.t1

MEKRVVDQSKSEFSSTVLGFLNKFLMEDDDIEEDYNMFQHNEHEESSNALDSINQFLMEEDFEKKYTMFQDSFALQVTEKSFHDAIAHTPLPPPTIAAAVTAITTTLPSFSIQHEYQQNYNFVHSPNFSDYSLSSGSTSSFELDPHIDSYNYNYNPFLLPNTPPFSPNNFVSQSNSTFFPSFNNALSHEVLQTENFEKEHFLNVSQNGSEQVYVDDSSELSELFDKVLVLGTKFTKGPLQNTSFQQNEESSNRFYRSRKRSYEEVVDLRTLLMLCAQSISCNDISNANQLLNQIKKHSSTTGDGTQKLAHFFGNALEARLAGTGSHIYRALSSKKKSAADMVKAYQVYSSACPFEKLAIMFSNDAILNVTKETESLHIIDFGVGYGFKWLGFIYRLSKRSGGPPKLRITGIDLPNSLERVNETGLRLASYCKRFNVPFECNGIAKNWESIKVQDFKIRKNEFVAVTCVFKFENLPDETVVSENPRGAVLDLIKKANPNIFIHSIVNGGYDAPFFVTRFKEAVFYYSALFDMLDNNNVECEDPVRLMFEEDVWGKDIMNVIACEGCDRVQRPETYMQWHSRHMGNGFRSLKLDKQIINKLKGKLRDDAYNSDFLFEVKENWMLQGWKGRILFGSSCWIPA