AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052262

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052262.t1 MTR_4g075250 96.377 138 5 0 125 262 274 411 2.56e-92 278
MS.gene052262.t1 MTR_4g075250 100.000 124 0 0 1 124 1 124 6.46e-87 265
MS.gene052262.t1 MTR_5g075880 60.204 98 38 1 28 124 38 135 2.09e-31 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052262.t1 AT3G12400 74.400 125 31 1 1 124 1 125 1.36e-64 207
MS.gene052262.t1 AT3G12400 64.885 131 45 1 125 254 263 393 9.69e-54 179
MS.gene052262.t1 AT3G12400 74.400 125 31 1 1 124 1 125 1.48e-64 207
MS.gene052262.t1 AT3G12400 64.444 135 47 1 125 258 263 397 2.48e-55 183
MS.gene052262.t1 AT3G12400 74.400 125 31 1 1 124 1 125 3.21e-64 207
MS.gene052262.t1 AT3G12400 64.885 131 45 1 125 254 263 393 1.89e-53 179
MS.gene052262.t1 AT5G13860 71.200 125 35 1 1 124 1 125 5.88e-59 191
MS.gene052262.t1 AT5G13860 63.235 136 48 1 125 258 232 367 1.16e-52 175
MS.gene052262.t1 AT2G38830 40.517 116 62 4 12 123 12 124 3.49e-20 88.6

Find 97 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAGGAATTGTTGGGTTTC+GGG 0.148763 4.2:-33583736 None:intergenic
AATTGTTGGGTTTCGGGGTT+TGG 0.182716 4.2:-33583730 None:intergenic
CATCCTCATGTTAATCCTTC+TGG 0.204058 4.2:+33584060 MS.gene052262:CDS
CCCAATTGTTATCTGGCTTA+TGG 0.211522 4.2:+33583969 MS.gene052262:CDS
ATTGTTGGGTTTCGGGGTTT+GGG 0.228262 4.2:-33583729 None:intergenic
GTGAGTGTAGGTGGCGGTTT+TGG 0.235521 4.2:-33583869 None:intergenic
GCTGAGGAATTGTTGGGTTT+CGG 0.263335 4.2:-33583737 None:intergenic
TGCTTTAGATAAGGGTGTTC+AGG 0.272232 4.2:+33584773 MS.gene052262:CDS
GGAGAATCAAGGGAAGAAAA+TGG 0.279130 4.2:+33584647 MS.gene052262:CDS
GCAGGCTCAGGTTGCGAATA+TGG 0.279429 4.2:+33584899 MS.gene052262:CDS
TAGATAAGGGTGTTCAGGTT+GGG 0.287353 4.2:+33584778 MS.gene052262:CDS
AGGGAAGAAAATGGGGAGTT+TGG 0.299911 4.2:+33584656 MS.gene052262:CDS
TGATTGTACTTCTGCTGATT+TGG 0.313474 4.2:+33584731 MS.gene052262:CDS
TTTGATGAATACCGATGTTT+TGG 0.314875 4.2:+33584611 MS.gene052262:CDS
ACAACATCCCAATTGTTATC+TGG 0.318668 4.2:+33583962 MS.gene052262:CDS
TTGTTGGGTTTCGGGGTTTG+GGG 0.329175 4.2:-33583728 None:intergenic
GAGAATCAAGGGAAGAAAAT+GGG 0.338918 4.2:+33584648 MS.gene052262:CDS
AGTTAGAGCTGCTCAGTTGC+AGG 0.360251 4.2:+33584881 MS.gene052262:CDS
ACGCTTTATGCTTTAGATAA+GGG 0.364897 4.2:+33584765 MS.gene052262:CDS
TCCATAAGCCAGATAACAAT+TGG 0.369758 4.2:-33583970 None:intergenic
GCAAGATGAGATGGAGGGTT+TGG 0.370726 4.2:+33584569 MS.gene052262:CDS
AGCTGCTCAGTTGCAGGCTC+AGG 0.393395 4.2:+33584887 MS.gene052262:CDS
AGATAAGGGTGTTCAGGTTG+GGG 0.397945 4.2:+33584779 MS.gene052262:CDS
CATTTGGTGTCTTCGGAGTA+AGG 0.400855 4.2:-33583793 None:intergenic
TACGCTTTATGCTTTAGATA+AGG 0.408278 4.2:+33584764 MS.gene052262:CDS
GAGAGCGGAGGGGCCGCGTT+GGG 0.413398 4.2:-33583770 None:intergenic
GGATTGAGCTGAGGAATTGT+TGG 0.417142 4.2:-33583744 None:intergenic
TCATGTTAATCCTTCTGGAT+TGG 0.419158 4.2:+33584065 MS.gene052262:CDS
GCTGCTAGGAATCATCATTA+TGG 0.423124 4.2:+33584921 MS.gene052262:CDS
GGTATTGAAGCAGAGGGAGG+AGG 0.425612 4.2:+33584521 MS.gene052262:intron
TACCGTTTGATCAGTATTTG+AGG 0.441585 4.2:+33584805 MS.gene052262:CDS
GATTGAGCTGAGGAATTGTT+GGG 0.455019 4.2:-33583743 None:intergenic
CTCCTCAAATACTGATCAAA+CGG 0.461994 4.2:-33584807 None:intergenic
CGGAGGGGCCGCGTTGGGAG+AGG 0.462809 4.2:-33583765 None:intergenic
GAAGAGAGAGGAGATGTTGA+CGG 0.466235 4.2:-33583822 None:intergenic
GATAAGCCATTTGGTGTCTT+CGG 0.468483 4.2:-33583800 None:intergenic
GGAGATGCAAGATGAGATGG+AGG 0.478519 4.2:+33584563 MS.gene052262:CDS
TTTAATAATCATATCCCTAG+TGG 0.481041 4.2:-33584031 None:intergenic
GATAACAATTGGGATGTTGT+AGG 0.486615 4.2:-33583959 None:intergenic
GGATTGTGCCATCGGCTTGA+AGG 0.490820 4.2:-33583915 None:intergenic
TTAGATAAGGGTGTTCAGGT+TGG 0.491802 4.2:+33584777 MS.gene052262:CDS
GGTTGGTTGAGGGAGAATCA+AGG 0.499129 4.2:+33584636 MS.gene052262:CDS
GGAGAGCGGAGGGGCCGCGT+TGG 0.502152 4.2:-33583771 None:intergenic
TCGGAGTAAGGGAGAGCGGA+GGG 0.508533 4.2:-33583781 None:intergenic
ATGTTGACGGATAAGCCATT+TGG 0.510171 4.2:-33583809 None:intergenic
GGTCATGGGGATTGTGCCAT+CGG 0.512307 4.2:-33583923 None:intergenic
GTGACGGATTGGTAGGTCAT+GGG 0.514713 4.2:-33583937 None:intergenic
GTTGGTTGAGGGAGAATCAA+GGG 0.518369 4.2:+33584637 MS.gene052262:CDS
CTTACTCCGAAGACACCAAA+TGG 0.520060 4.2:+33583794 MS.gene052262:CDS
GAGTGTGAGAGCGTTGTCGA+GGG 0.528786 4.2:+33584827 MS.gene052262:CDS
TGTTGGGTTTCGGGGTTTGG+GGG 0.530033 4.2:-33583727 None:intergenic
CCATAAGCCAGATAACAATT+GGG 0.531355 4.2:-33583969 None:intergenic
GAAGGAGATGCAAGATGAGA+TGG 0.537662 4.2:+33584560 MS.gene052262:CDS
AGAATCAAGGGAAGAAAATG+GGG 0.538001 4.2:+33584649 MS.gene052262:CDS
GGGTTCGAGAGAAGGGAAGG+TGG 0.544802 4.2:-33583848 None:intergenic
GGAAGGTGGTGAGAAGAGAG+AGG 0.546745 4.2:-33583834 None:intergenic
TTTGGGTTCGAGAGAAGGGA+AGG 0.549016 4.2:-33583851 None:intergenic
AATTGGGATGTTGTAGGTGA+CGG 0.550105 4.2:-33583953 None:intergenic
GTCAATCTCCTTCAAGCCGA+TGG 0.556533 4.2:+33583907 MS.gene052262:CDS
TTTGGAGCAGCAGTTGCAGA+TGG 0.557427 4.2:+33584587 MS.gene052262:CDS
GCAGTTCTTCCACCGTGCTA+CGG 0.559208 4.2:+33584851 MS.gene052262:CDS
GGGGAGTTTGGAGAATGTTG+AGG 0.560828 4.2:+33584668 MS.gene052262:CDS
TTAATAATCATATCCCTAGT+GGG 0.565302 4.2:-33584030 None:intergenic
GGTGACGGATTGGTAGGTCA+TGG 0.566703 4.2:-33583938 None:intergenic
GCGGCCGTCGTTGTGAGTGT+AGG 0.567824 4.2:-33583881 None:intergenic
GGATGTTGTAGGTGACGGAT+TGG 0.570458 4.2:-33583948 None:intergenic
ATCGGCTTGAAGGAGATTGA+CGG 0.570840 4.2:-33583905 None:intergenic
GTTGTAGGTGACGGATTGGT+AGG 0.571249 4.2:-33583944 None:intergenic
GTGTTTATGTTAATCCCACT+AGG 0.576064 4.2:+33584016 MS.gene052262:CDS
GGAGTGTGAGAGCGTTGTCG+AGG 0.576375 4.2:+33584826 MS.gene052262:CDS
GCCGTCGTTGTGAGTGTAGG+TGG 0.582716 4.2:-33583878 None:intergenic
TGAGGAATTGTTGGGTTTCG+GGG 0.583538 4.2:-33583735 None:intergenic
GAGTTTGGAGAATGTTGAGG+AGG 0.585981 4.2:+33584671 MS.gene052262:CDS
ATTTGGTGTCTTCGGAGTAA+GGG 0.586991 4.2:-33583792 None:intergenic
AGGTTGCGAATATGGCTGCT+AGG 0.587435 4.2:+33584907 MS.gene052262:CDS
TGTTTATGTTAATCCCACTA+GGG 0.610790 4.2:+33584017 MS.gene052262:CDS
TGACGGATTGGTAGGTCATG+GGG 0.614345 4.2:-33583936 None:intergenic
TTCGGAGTAAGGGAGAGCGG+AGG 0.618360 4.2:-33583782 None:intergenic
AATCATCATTATGGTAGCTG+AGG 0.620092 4.2:+33584930 MS.gene052262:CDS
TAAACACGAGGAGGGTGACG+AGG 0.622293 4.2:-33584000 None:intergenic
GTCGTTGTGAGTGTAGGTGG+CGG 0.624469 4.2:-33583875 None:intergenic
TAACTTTAGCAGCCGTAGCA+CGG 0.627369 4.2:-33584863 None:intergenic
GTCTTCGGAGTAAGGGAGAG+CGG 0.628756 4.2:-33583785 None:intergenic
GCCACCTACACTCACAACGA+CGG 0.639409 4.2:+33583877 MS.gene052262:CDS
GGAACAGAAACCAATCCAGA+AGG 0.646246 4.2:-33584075 None:intergenic
CAACCAACCCTCCAAAACAT+CGG 0.653851 4.2:-33584622 None:intergenic
GAGATGCAAGATGAGATGGA+GGG 0.654104 4.2:+33584564 MS.gene052262:CDS
GGATTAACATAAACACGAGG+AGG 0.659983 4.2:-33584009 None:intergenic
AAACACGAGGAGGGTGACGA+GGG 0.665903 4.2:-33583999 None:intergenic
CGGAGTAAGGGAGAGCGGAG+GGG 0.668045 4.2:-33583780 None:intergenic
AATCCAGAAGGATTAACATG+AGG 0.672810 4.2:-33584063 None:intergenic
GTTGGGAGAGGATTGAGCTG+AGG 0.683317 4.2:-33583753 None:intergenic
CTTGAAGGAGATTGACGGCG+CGG 0.686041 4.2:-33583900 None:intergenic
AGCTCAATCCTCTCCCAACG+CGG 0.705583 4.2:+33583757 MS.gene052262:CDS
GATTAACATAAACACGAGGA+GGG 0.706994 4.2:-33584008 None:intergenic
CTTTAGCAGCCGTAGCACGG+TGG 0.725711 4.2:-33584860 None:intergenic
GTGGGATTAACATAAACACG+AGG 0.780290 4.2:-33584012 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TAAATCCAGTTATGCAAATA+CGG - chr4.2:33584099-33584118 None:intergenic 25.0%
! TCCAAAATTAGAACTTTGAT+TGG - chr4.2:33584265-33584284 None:intergenic 25.0%
! TTAATAATCATATCCCTAGT+GGG - chr4.2:33584033-33584052 None:intergenic 25.0%
! TTAGAACTTTGATTGGTATT+TGG - chr4.2:33584258-33584277 None:intergenic 25.0%
! TTATTACTATTAATCCTACC+AGG - chr4.2:33584315-33584334 None:intergenic 25.0%
! TTTAATAATCATATCCCTAG+TGG - chr4.2:33584034-33584053 None:intergenic 25.0%
!!! ACCAATCAAAGTTCTAATTT+TGG + chr4.2:33584261-33584280 MS.gene052262:intron 25.0%
!!! TATGATTTTGATGAGGATTA+GGG - chr4.2:33584227-33584246 None:intergenic 25.0%
!!! TTAGGATTATGATTTTGATG+AGG - chr4.2:33584234-33584253 None:intergenic 25.0%
!!! TTATGATTTTGATGAGGATT+AGG - chr4.2:33584228-33584247 None:intergenic 25.0%
AGATCAACCAAATTAGAACT+AGG - chr4.2:33584123-33584142 None:intergenic 30.0%
CTTTGATTGGTATTTGGATT+AGG - chr4.2:33584252-33584271 None:intergenic 30.0%
GATCAACCAAATTAGAACTA+GGG - chr4.2:33584122-33584141 None:intergenic 30.0%
TGTTTATGTTAATCCCACTA+GGG + chr4.2:33584017-33584036 MS.gene052262:CDS 30.0%
! ACGCTTTATGCTTTAGATAA+GGG + chr4.2:33584765-33584784 MS.gene052262:CDS 30.0%
! TACGCTTTATGCTTTAGATA+AGG + chr4.2:33584764-33584783 MS.gene052262:CDS 30.0%
! TTTGATGAATACCGATGTTT+TGG + chr4.2:33584611-33584630 MS.gene052262:CDS 30.0%
!! CTTGCTTTGAGTTTGATTTT+TGG + chr4.2:33584147-33584166 MS.gene052262:intron 30.0%
!! GATTTACCCTAGTTCTAATT+TGG + chr4.2:33584113-33584132 MS.gene052262:intron 30.0%
AATCCAGAAGGATTAACATG+AGG - chr4.2:33584066-33584085 None:intergenic 35.0%
ACAACATCCCAATTGTTATC+TGG + chr4.2:33583962-33583981 MS.gene052262:CDS 35.0%
AGAATCAAGGGAAGAAAATG+GGG + chr4.2:33584649-33584668 MS.gene052262:CDS 35.0%
ATTAGGGTTAGGATTAGGAT+TGG - chr4.2:33584211-33584230 None:intergenic 35.0%
CCATAAGCCAGATAACAATT+GGG - chr4.2:33583972-33583991 None:intergenic 35.0%
CTCCTCAAATACTGATCAAA+CGG - chr4.2:33584810-33584829 None:intergenic 35.0%
GAATGCTATTAACAAGCTTG+TGG + chr4.2:33584422-33584441 MS.gene052262:intron 35.0%
GAGAATCAAGGGAAGAAAAT+GGG + chr4.2:33584648-33584667 MS.gene052262:CDS 35.0%
GATAACAATTGGGATGTTGT+AGG - chr4.2:33583962-33583981 None:intergenic 35.0%
GATTAACATAAACACGAGGA+GGG - chr4.2:33584011-33584030 None:intergenic 35.0%
GTGTTTATGTTAATCCCACT+AGG + chr4.2:33584016-33584035 MS.gene052262:CDS 35.0%
TACCGTTTGATCAGTATTTG+AGG + chr4.2:33584805-33584824 MS.gene052262:CDS 35.0%
TATTAACAAGCTTGTGGAGA+TGG + chr4.2:33584428-33584447 MS.gene052262:intron 35.0%
TCATGTTAATCCTTCTGGAT+TGG + chr4.2:33584065-33584084 MS.gene052262:CDS 35.0%
TCCATAAGCCAGATAACAAT+TGG - chr4.2:33583973-33583992 None:intergenic 35.0%
TTAGGATTAGGATTGGGATT+AGG - chr4.2:33584204-33584223 None:intergenic 35.0%
TTAGGGTTAGGATTAGGATT+GGG - chr4.2:33584210-33584229 None:intergenic 35.0%
! TGATTGTACTTCTGCTGATT+TGG + chr4.2:33584731-33584750 MS.gene052262:CDS 35.0%
! TTTTGATGAGGATTAGGGTT+AGG - chr4.2:33584222-33584241 None:intergenic 35.0%
!! ATGAATACCGATGTTTTGGA+GGG + chr4.2:33584615-33584634 MS.gene052262:CDS 35.0%
!!! GGTTTTGAATAATGGTGTGA+AGG + chr4.2:33584542-33584561 MS.gene052262:CDS 35.0%
AATTGGGATGTTGTAGGTGA+CGG - chr4.2:33583956-33583975 None:intergenic 40.0%
ACTAGAGAAGGTGAAATGGA+GGG + chr4.2:33584480-33584499 MS.gene052262:intron 40.0%
CATCCTCATGTTAATCCTTC+TGG + chr4.2:33584060-33584079 MS.gene052262:CDS 40.0%
CTACGTTGAGAAAAAAGAGG+TGG - chr4.2:33584180-33584199 None:intergenic 40.0%
CTGTTCCGTATTTGCATAAC+TGG + chr4.2:33584091-33584110 MS.gene052262:intron 40.0%
GAAGACTAGAGAAGGTGAAA+TGG + chr4.2:33584476-33584495 MS.gene052262:intron 40.0%
GATGGTGCATAATGATGTGA+CGG + chr4.2:33584446-33584465 MS.gene052262:intron 40.0%
GATTGAGCTGAGGAATTGTT+GGG - chr4.2:33583746-33583765 None:intergenic 40.0%
GCTGCTAGGAATCATCATTA+TGG + chr4.2:33584921-33584940 MS.gene052262:CDS 40.0%
GGAGAATCAAGGGAAGAAAA+TGG + chr4.2:33584647-33584666 MS.gene052262:CDS 40.0%
GGATTAACATAAACACGAGG+AGG - chr4.2:33584012-33584031 None:intergenic 40.0%
GGTCTACGTTGAGAAAAAAG+AGG - chr4.2:33584183-33584202 None:intergenic 40.0%
GTGGGATTAACATAAACACG+AGG - chr4.2:33584015-33584034 None:intergenic 40.0%
TGAGGATTAGGGTTAGGATT+AGG - chr4.2:33584216-33584235 None:intergenic 40.0%
TTCCTCCTAAAAACCTCAGT+AGG - chr4.2:33584405-33584424 None:intergenic 40.0%
TTGGAACTAATCCTCCATCT+GGG + chr4.2:33584280-33584299 MS.gene052262:intron 40.0%
! AGGATCCTACTGAGGTTTTT+AGG + chr4.2:33584397-33584416 MS.gene052262:intron 40.0%
! ATGTTGACGGATAAGCCATT+TGG - chr4.2:33583812-33583831 None:intergenic 40.0%
! CCCAATTGTTATCTGGCTTA+TGG + chr4.2:33583969-33583988 MS.gene052262:CDS 40.0%
! TTTGGAACTAATCCTCCATC+TGG + chr4.2:33584279-33584298 MS.gene052262:intron 40.0%
!! AGGGATTGTTTGGTTTGCAA+GGG + chr4.2:33584499-33584518 MS.gene052262:intron 40.0%
!! ATCCTACTGAGGTTTTTAGG+AGG + chr4.2:33584400-33584419 MS.gene052262:intron 40.0%
!! ATTTGGTGTCTTCGGAGTAA+GGG - chr4.2:33583795-33583814 None:intergenic 40.0%
!! GATAAGCCATTTGGTGTCTT+CGG - chr4.2:33583803-33583822 None:intergenic 40.0%
!! GATGAATACCGATGTTTTGG+AGG + chr4.2:33584614-33584633 MS.gene052262:CDS 40.0%
!! TAGATAAGGGTGTTCAGGTT+GGG + chr4.2:33584778-33584797 MS.gene052262:CDS 40.0%
!! TGCTTTAGATAAGGGTGTTC+AGG + chr4.2:33584773-33584792 MS.gene052262:CDS 40.0%
!! TTAGATAAGGGTGTTCAGGT+TGG + chr4.2:33584777-33584796 MS.gene052262:CDS 40.0%
!!! AGGGAGGAGGTTTTGAATAA+TGG + chr4.2:33584534-33584553 MS.gene052262:CDS 40.0%
AATTGTTGGGTTTCGGGGTT+TGG - chr4.2:33583733-33583752 None:intergenic 45.0%
AGGGAAGAAAATGGGGAGTT+TGG + chr4.2:33584656-33584675 MS.gene052262:CDS 45.0%
ATAATGATGTGACGGCGTTG+AGG + chr4.2:33584454-33584473 MS.gene052262:intron 45.0%
ATTGTTGGGTTTCGGGGTTT+GGG - chr4.2:33583732-33583751 None:intergenic 45.0%
CAACCAACCCTCCAAAACAT+CGG - chr4.2:33584625-33584644 None:intergenic 45.0%
CCAGGATGAATATACCCAGA+TGG - chr4.2:33584297-33584316 None:intergenic 45.0%
CCATCTGGGTATATTCATCC+TGG + chr4.2:33584294-33584313 MS.gene052262:intron 45.0%
CTGAGGAATTGTTGGGTTTC+GGG - chr4.2:33583739-33583758 None:intergenic 45.0%
CTGGGTATATTCATCCTGGT+AGG + chr4.2:33584298-33584317 MS.gene052262:intron 45.0%
CTTACTCCGAAGACACCAAA+TGG + chr4.2:33583794-33583813 MS.gene052262:CDS 45.0%
GAAGAGAGAGGAGATGTTGA+CGG - chr4.2:33583825-33583844 None:intergenic 45.0%
GAAGGAGATGCAAGATGAGA+TGG + chr4.2:33584560-33584579 MS.gene052262:CDS 45.0%
GACTAGAGAAGGTGAAATGG+AGG + chr4.2:33584479-33584498 MS.gene052262:intron 45.0%
GAGATGCAAGATGAGATGGA+GGG + chr4.2:33584564-33584583 MS.gene052262:CDS 45.0%
GAGTTTGGAGAATGTTGAGG+AGG + chr4.2:33584671-33584690 MS.gene052262:CDS 45.0%
GCTGAGGAATTGTTGGGTTT+CGG - chr4.2:33583740-33583759 None:intergenic 45.0%
GGAACAGAAACCAATCCAGA+AGG - chr4.2:33584078-33584097 None:intergenic 45.0%
GGATGAATATACCCAGATGG+AGG - chr4.2:33584294-33584313 None:intergenic 45.0%
GGATTGAGCTGAGGAATTGT+TGG - chr4.2:33583747-33583766 None:intergenic 45.0%
GGTGAAATGGAGGGATTGTT+TGG + chr4.2:33584489-33584508 MS.gene052262:intron 45.0%
GTTGGTTGAGGGAGAATCAA+GGG + chr4.2:33584637-33584656 MS.gene052262:CDS 45.0%
TAACTTTAGCAGCCGTAGCA+CGG - chr4.2:33584866-33584885 None:intergenic 45.0%
TGAGGAATTGTTGGGTTTCG+GGG - chr4.2:33583738-33583757 None:intergenic 45.0%
!! AGATAAGGGTGTTCAGGTTG+GGG + chr4.2:33584779-33584798 MS.gene052262:CDS 45.0%
!! ATCGGCTTGAAGGAGATTGA+CGG - chr4.2:33583908-33583927 None:intergenic 45.0%
!! CATTTGGTGTCTTCGGAGTA+AGG - chr4.2:33583796-33583815 None:intergenic 45.0%
!! GAGGGATTGTTTGGTTTGCA+AGG + chr4.2:33584498-33584517 MS.gene052262:intron 45.0%
!! GGGATTGTTTGGTTTGCAAG+GGG + chr4.2:33584500-33584519 MS.gene052262:intron 45.0%
!!! ATACCGATGTTTTGGAGGGT+TGG + chr4.2:33584619-33584638 MS.gene052262:CDS 45.0%
!!! ATGTTTTGGAGGGTTGGTTG+AGG + chr4.2:33584625-33584644 MS.gene052262:CDS 45.0%
!!! TGTTTTGGAGGGTTGGTTGA+GGG + chr4.2:33584626-33584645 MS.gene052262:CDS 45.0%
AGGTTGCGAATATGGCTGCT+AGG + chr4.2:33584907-33584926 MS.gene052262:CDS 50.0%
AGTTAGAGCTGCTCAGTTGC+AGG + chr4.2:33584881-33584900 MS.gene052262:CDS 50.0%
GCAAGATGAGATGGAGGGTT+TGG + chr4.2:33584569-33584588 MS.gene052262:CDS 50.0%
GCGTTGAGGAAGACTAGAGA+AGG + chr4.2:33584468-33584487 MS.gene052262:intron 50.0%
GGAGATGCAAGATGAGATGG+AGG + chr4.2:33584563-33584582 MS.gene052262:CDS 50.0%
GGGGAGTTTGGAGAATGTTG+AGG + chr4.2:33584668-33584687 MS.gene052262:CDS 50.0%
GTCAATCTCCTTCAAGCCGA+TGG + chr4.2:33583907-33583926 MS.gene052262:CDS 50.0%
TTGTTGGGTTTCGGGGTTTG+GGG - chr4.2:33583731-33583750 None:intergenic 50.0%
! GGTTGGTTGAGGGAGAATCA+AGG + chr4.2:33584636-33584655 MS.gene052262:CDS 50.0%
! TTTGGAGCAGCAGTTGCAGA+TGG + chr4.2:33584587-33584606 MS.gene052262:CDS 50.0%
! TTTGGGTTCGAGAGAAGGGA+AGG - chr4.2:33583854-33583873 None:intergenic 50.0%
!! GCAAGGGGTATTGAAGCAGA+GGG + chr4.2:33584515-33584534 MS.gene052262:intron 50.0%
!! GGATGTTGTAGGTGACGGAT+TGG - chr4.2:33583951-33583970 None:intergenic 50.0%
!! GTGACGGATTGGTAGGTCAT+GGG - chr4.2:33583940-33583959 None:intergenic 50.0%
!! GTTGTAGGTGACGGATTGGT+AGG - chr4.2:33583947-33583966 None:intergenic 50.0%
!! TGACGGATTGGTAGGTCATG+GGG - chr4.2:33583939-33583958 None:intergenic 50.0%
!! TGAGGAGGCTTTTGAGTGTG+TGG + chr4.2:33584686-33584705 MS.gene052262:CDS 50.0%
!! TGAGTGTAGGTGGCGGTTTT+GGG - chr4.2:33583871-33583890 None:intergenic 50.0%
!! TGCAAGGGGTATTGAAGCAG+AGG + chr4.2:33584514-33584533 MS.gene052262:intron 50.0%
!!! CGGTTTTGGGTTCGAGAGAA+GGG - chr4.2:33583858-33583877 None:intergenic 50.0%
AAACACGAGGAGGGTGACGA+GGG - chr4.2:33584002-33584021 None:intergenic 55.0%
AGCTCAATCCTCTCCCAACG+CGG + chr4.2:33583757-33583776 MS.gene052262:CDS 55.0%
CTTATTCGCCTGCGCCATCT+CGG + chr4.2:33584361-33584380 MS.gene052262:intron 55.0%
CTTGAAGGAGATTGACGGCG+CGG - chr4.2:33583903-33583922 None:intergenic 55.0%
GCAGGCGAATAAGGTGAAGG+AGG - chr4.2:33584354-33584373 None:intergenic 55.0%
GCAGTTCTTCCACCGTGCTA+CGG + chr4.2:33584851-33584870 MS.gene052262:CDS 55.0%
GCATACGGAGGATCCTACTG+AGG + chr4.2:33584389-33584408 MS.gene052262:intron 55.0%
GCCACCTACACTCACAACGA+CGG + chr4.2:33583877-33583896 MS.gene052262:CDS 55.0%
GGAAGGTGGTGAGAAGAGAG+AGG - chr4.2:33583837-33583856 None:intergenic 55.0%
GGTCATGGGGATTGTGCCAT+CGG - chr4.2:33583926-33583945 None:intergenic 55.0%
GTAGGATCCTCCGTATGCGT+AGG - chr4.2:33584387-33584406 None:intergenic 55.0%
GTCGTTGTGAGTGTAGGTGG+CGG - chr4.2:33583878-33583897 None:intergenic 55.0%
GTCTTCGGAGTAAGGGAGAG+CGG - chr4.2:33583788-33583807 None:intergenic 55.0%
GTTGGGAGAGGATTGAGCTG+AGG - chr4.2:33583756-33583775 None:intergenic 55.0%
TAAACACGAGGAGGGTGACG+AGG - chr4.2:33584003-33584022 None:intergenic 55.0%
TGTTGGGTTTCGGGGTTTGG+GGG - chr4.2:33583730-33583749 None:intergenic 55.0%
! GCAGGCTCAGGTTGCGAATA+TGG + chr4.2:33584899-33584918 MS.gene052262:CDS 55.0%
! GGTATTGAAGCAGAGGGAGG+AGG + chr4.2:33584521-33584540 MS.gene052262:intron 55.0%
!! AGGGGTATTGAAGCAGAGGG+AGG + chr4.2:33584518-33584537 MS.gene052262:intron 55.0%
!! GAGTGTGAGAGCGTTGTCGA+GGG + chr4.2:33584827-33584846 MS.gene052262:CDS 55.0%
!! GGATTGTGCCATCGGCTTGA+AGG - chr4.2:33583918-33583937 None:intergenic 55.0%
!! GGTGACGGATTGGTAGGTCA+TGG - chr4.2:33583941-33583960 None:intergenic 55.0%
!! GTGAGTGTAGGTGGCGGTTT+TGG - chr4.2:33583872-33583891 None:intergenic 55.0%
!!! GCGGTTTTGGGTTCGAGAGA+AGG - chr4.2:33583859-33583878 None:intergenic 55.0%
AGCTGCTCAGTTGCAGGCTC+AGG + chr4.2:33584887-33584906 MS.gene052262:CDS 60.0%
ATCTCGGCCTACGCATACGG+AGG + chr4.2:33584377-33584396 MS.gene052262:intron 60.0%
CGAGATGGCGCAGGCGAATA+AGG - chr4.2:33584363-33584382 None:intergenic 60.0%
CTTTAGCAGCCGTAGCACGG+TGG - chr4.2:33584863-33584882 None:intergenic 60.0%
GCCATCTCGGCCTACGCATA+CGG + chr4.2:33584374-33584393 MS.gene052262:intron 60.0%
GCCGTCGTTGTGAGTGTAGG+TGG - chr4.2:33583881-33583900 None:intergenic 60.0%
GGCGCAGGCGAATAAGGTGA+AGG - chr4.2:33584357-33584376 None:intergenic 60.0%
GGGTTCGAGAGAAGGGAAGG+TGG - chr4.2:33583851-33583870 None:intergenic 60.0%
TCCGTATGCGTAGGCCGAGA+TGG - chr4.2:33584378-33584397 None:intergenic 60.0%
TCGGAGTAAGGGAGAGCGGA+GGG - chr4.2:33583784-33583803 None:intergenic 60.0%
TTCGGAGTAAGGGAGAGCGG+AGG - chr4.2:33583785-33583804 None:intergenic 60.0%
!! GGAGTGTGAGAGCGTTGTCG+AGG + chr4.2:33584826-33584845 MS.gene052262:CDS 60.0%
CGGAGTAAGGGAGAGCGGAG+GGG - chr4.2:33583783-33583802 None:intergenic 65.0%
GCGGCCGTCGTTGTGAGTGT+AGG - chr4.2:33583884-33583903 None:intergenic 65.0%
GTTTCGGGGTTTGGGGGTGG+TGG - chr4.2:33583724-33583743 None:intergenic 65.0%
TGGGTTTCGGGGTTTGGGGG+TGG - chr4.2:33583727-33583746 None:intergenic 65.0%
TGCGTAGGCCGAGATGGCGC+AGG - chr4.2:33584372-33584391 None:intergenic 70.0%
GAGAGCGGAGGGGCCGCGTT+GGG - chr4.2:33583773-33583792 None:intergenic 75.0%
! CGGAGGGGCCGCGTTGGGAG+AGG - chr4.2:33583768-33583787 None:intergenic 80.0%
! GGAGAGCGGAGGGGCCGCGT+TGG - chr4.2:33583774-33583793 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 33583715 33584950 33583715 ID=MS.gene052262
chr4.2 mRNA 33583715 33584950 33583715 ID=MS.gene052262.t1;Parent=MS.gene052262
chr4.2 exon 33583715 33584086 33583715 ID=MS.gene052262.t1.exon1;Parent=MS.gene052262.t1
chr4.2 CDS 33583715 33584086 33583715 ID=cds.MS.gene052262.t1;Parent=MS.gene052262.t1
chr4.2 exon 33584534 33584950 33584534 ID=MS.gene052262.t1.exon2;Parent=MS.gene052262.t1
chr4.2 CDS 33584534 33584950 33584534 ID=cds.MS.gene052262.t1;Parent=MS.gene052262.t1
Gene Sequence

>MS.gene052262

ATGGTTCCACCACCCCCAAACCCCGAAACCCAACAATTCCTCAGCTCAATCCTCTCCCAACGCGGCCCCTCCGCTCTCCCTTACTCCGAAGACACCAAATGGCTTATCCGTCAACATCTCCTCTCTCTTCTCACCACCTTCCCTTCTCTCGAACCCAAAACCGCCACCTACACTCACAACGACGGCCGCGCCGTCAATCTCCTTCAAGCCGATGGCACAATCCCCATGACCTACCAATCCGTCACCTACAACATCCCAATTGTTATCTGGCTTATGGAATCTTACCCTCGTCACCCTCCTCGTGTTTATGTTAATCCCACTAGGGATATGATTATTAAACATGCTCATCCTCATGTTAATCCTTCTGGATTGGTTTCTGTTCCGTATTTGCATAACTGGATTTACCCTAGTTCTAATTTGGTTGATCTTGTTCTTGCTTTGAGTTTGATTTTTGGTCGTGATCCACCTCTTTTTTCTCAACGTAGACCTAATCCCAATCCTAATCCTAACCCTAATCCTCATCAAAATCATAATCCTAATCCAAATACCAATCAAAGTTCTAATTTTGGAACTAATCCTCCATCTGGGTATATTCATCCTGGTAGGATTAATAGTAATAATAATATCAATAGTTATCCTCCTTCACCTTATTCGCCTGCGCCATCTCGGCCTACGCATACGGAGGATCCTACTGAGGTTTTTAGGAGGAATGCTATTAACAAGCTTGTGGAGATGGTGCATAATGATGTGACGGCGTTGAGGAAGACTAGAGAAGGTGAAATGGAGGGATTGTTTGGTTTGCAAGGGGTATTGAAGCAGAGGGAGGAGGTTTTGAATAATGGTGTGAAGGAGATGCAAGATGAGATGGAGGGTTTGGAGCAGCAGTTGCAGATGGTTTTGATGAATACCGATGTTTTGGAGGGTTGGTTGAGGGAGAATCAAGGGAAGAAAATGGGGAGTTTGGAGAATGTTGAGGAGGCTTTTGAGTGTGTGGATGTGCTTTCTAAACAGATGCTTGATTGTACTTCTGCTGATTTGGCTATTGAAGATACGCTTTATGCTTTAGATAAGGGTGTTCAGGTTGGGGCTGTACCGTTTGATCAGTATTTGAGGAGTGTGAGAGCGTTGTCGAGGGAGCAGTTCTTCCACCGTGCTACGGCTGCTAAAGTTAGAGCTGCTCAGTTGCAGGCTCAGGTTGCGAATATGGCTGCTAGGAATCATCATTATGGTAGCTGA
Protein sequence

>MS.gene052262.t1

MVPPPPNPETQQFLSSILSQRGPSALPYSEDTKWLIRQHLLSLLTTFPSLEPKTATYTHNDGRAVNLLQADGTIPMTYQSVTYNIPIVIWLMESYPRHPPRVYVNPTRDMIIKHAHPHVNPSGLREEVLNNGVKEMQDEMEGLEQQLQMVLMNTDVLEGWLRENQGKKMGSLENVEEAFECVDVLSKQMLDCTSADLAIEDTLYALDKGVQVGAVPFDQYLRSVRALSREQFFHRATAAKVRAAQLQAQVANMAARNHHYGS