AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052317

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052317.t1 MTR_4g076470 94.020 301 18 0 1 301 29 329 0.0 576
MS.gene052317.t1 MTR_4g076490 85.000 300 44 1 1 299 103 402 0.0 523
MS.gene052317.t1 MTR_4g076440 82.724 301 52 0 1 301 29 329 0.0 520
MS.gene052317.t1 MTR_4g076430 84.385 301 47 0 1 301 29 329 0.0 519
MS.gene052317.t1 MTR_4g076255 70.333 300 87 2 3 301 33 331 4.54e-151 426
MS.gene052317.t1 MTR_4g076500 60.784 306 114 5 2 301 85 390 1.77e-123 358
MS.gene052317.t1 MTR_4g076570 55.446 303 128 4 2 297 29 331 2.01e-116 340
MS.gene052317.t1 MTR_1g052430 54.754 305 131 4 2 299 39 343 8.86e-112 329
MS.gene052317.t1 MTR_2g034480 53.896 308 133 4 1 300 33 339 7.38e-107 315
MS.gene052317.t1 MTR_2g034470 52.922 308 136 4 1 300 1 307 1.29e-105 311
MS.gene052317.t1 MTR_2g034470 52.922 308 136 4 1 300 36 342 2.23e-105 311
MS.gene052317.t1 MTR_2g034440 52.104 309 138 5 1 300 28 335 9.65e-102 301
MS.gene052317.t1 MTR_7g081350 43.046 302 161 8 3 296 35 333 3.75e-77 239
MS.gene052317.t1 MTR_5g085580 42.258 310 163 9 2 296 32 340 2.28e-73 233
MS.gene052317.t1 MTR_4g052970 39.159 309 173 7 2 296 36 343 2.63e-70 223
MS.gene052317.t1 MTR_3g080410 40.256 313 173 8 2 300 33 345 3.60e-70 221
MS.gene052317.t1 MTR_7g081370 40.468 299 160 7 3 296 36 321 2.81e-69 218
MS.gene052317.t1 MTR_4g010200 38.658 313 178 6 2 300 40 352 1.11e-68 219
MS.gene052317.t1 MTR_4g084480 39.286 308 174 7 2 296 39 346 9.89e-67 214
MS.gene052317.t1 MTR_4g084480 39.286 308 174 7 2 296 39 346 1.11e-66 213
MS.gene052317.t1 MTR_4g084480 39.286 308 174 7 2 296 32 339 1.15e-66 213
MS.gene052317.t1 MTR_1g077990 39.103 312 178 8 2 301 38 349 3.57e-66 213
MS.gene052317.t1 MTR_2g086530 36.688 308 180 6 2 296 15 320 4.18e-66 211
MS.gene052317.t1 MTR_2g086530 36.688 308 180 6 2 296 36 341 4.64e-66 212
MS.gene052317.t1 MTR_4g069940 37.898 314 181 7 2 301 36 349 4.38e-65 211
MS.gene052317.t1 MTR_6g088885 34.727 311 190 6 3 300 35 345 1.43e-63 208
MS.gene052317.t1 MTR_4g114850 35.032 314 190 7 2 301 33 346 1.99e-63 204
MS.gene052317.t1 MTR_4g114850 35.032 314 190 7 2 301 33 346 2.28e-63 204
MS.gene052317.t1 MTR_4g114850 35.032 314 190 7 2 301 33 346 2.53e-63 204
MS.gene052317.t1 MTR_1g031900 38.000 300 170 6 5 288 34 333 1.43e-62 206
MS.gene052317.t1 MTR_3g116510 37.134 307 179 7 4 296 37 343 2.46e-62 204
MS.gene052317.t1 MTR_1g031900 38.000 300 170 6 5 288 34 333 2.58e-62 205
MS.gene052317.t1 MTR_1g056640 37.864 309 178 7 2 296 45 353 4.82e-62 204
MS.gene052317.t1 MTR_1g022160 37.143 315 183 8 2 301 31 345 2.57e-61 200
MS.gene052317.t1 MTR_1g022160 37.742 310 178 8 2 296 31 340 1.10e-60 201
MS.gene052317.t1 MTR_1g022160 37.742 310 178 8 2 296 31 340 1.10e-60 200
MS.gene052317.t1 MTR_2g019345 38.312 308 176 7 2 296 31 337 1.10e-60 198
MS.gene052317.t1 MTR_1g022160 37.742 310 178 8 2 296 31 340 1.34e-60 200
MS.gene052317.t1 MTR_3g065460 37.086 302 174 6 4 289 33 334 1.51e-60 200
MS.gene052317.t1 MTR_1g007810 36.977 311 175 9 2 296 23 328 7.62e-60 195
MS.gene052317.t1 MTR_8g102340 38.247 251 143 5 62 300 62 312 2.26e-58 194
MS.gene052317.t1 MTR_5g078200 36.093 302 177 6 4 289 32 333 9.53e-58 196
MS.gene052317.t1 MTR_5g078200 36.333 300 175 6 6 289 1 300 1.01e-57 195
MS.gene052317.t1 MTR_7g053500 34.810 316 181 11 2 296 33 344 1.80e-56 189
MS.gene052317.t1 MTR_4g088155 33.438 317 188 9 1 296 29 343 2.01e-55 186
MS.gene052317.t1 MTR_8g012400 33.333 315 189 9 2 297 36 348 1.12e-54 184
MS.gene052317.t1 MTR_7g026340 34.727 311 184 9 2 298 30 335 3.30e-54 182
MS.gene052317.t1 MTR_7g085390 35.082 305 177 9 10 296 41 342 1.80e-53 181
MS.gene052317.t1 MTR_6g032820 34.983 303 178 8 12 296 43 344 4.62e-53 179
MS.gene052317.t1 MTR_3g083580 32.803 314 194 7 5 301 36 349 1.16e-52 180
MS.gene052317.t1 MTR_8g092070 33.770 305 186 8 12 301 51 354 2.07e-52 181
MS.gene052317.t1 MTR_4g132280 33.117 308 190 6 5 296 55 362 1.22e-50 175
MS.gene052317.t1 MTR_8g085720 30.990 313 200 6 4 300 35 347 3.24e-48 168
MS.gene052317.t1 MTR_4g124440 33.007 306 188 9 6 296 40 343 7.62e-48 167
MS.gene052317.t1 MTR_4g112025 33.645 321 180 11 1 297 38 349 9.18e-48 167
MS.gene052317.t1 MTR_7g053500 35.857 251 139 9 65 296 20 267 1.45e-46 161
MS.gene052317.t1 MTR_2g027560 33.882 304 179 10 12 296 45 345 3.17e-46 162
MS.gene052317.t1 MTR_8g012400 34.191 272 154 9 43 297 5 268 3.20e-46 160
MS.gene052317.t1 MTR_3g095050 33.115 305 186 10 12 301 51 352 6.09e-46 162
MS.gene052317.t1 MTR_3g116510 39.614 207 114 5 101 296 3 209 3.09e-45 156
MS.gene052317.t1 MTR_1g053320 31.847 314 196 9 1 297 37 349 3.09e-45 160
MS.gene052317.t1 MTR_1g010120 35.197 304 179 10 12 299 43 344 2.11e-44 157
MS.gene052317.t1 MTR_3g031770 33.887 301 183 11 11 297 43 341 1.02e-43 155
MS.gene052317.t1 MTR_4g083500 33.226 310 189 10 2 296 51 357 4.22e-43 155
MS.gene052317.t1 MTR_3g108600 33.766 308 169 10 5 297 41 328 1.94e-40 147
MS.gene052317.t1 MTR_4g036680 45.333 150 69 4 151 299 39 176 2.96e-33 122
MS.gene052317.t1 MTR_4g036680 52.632 57 27 0 2 58 29 85 2.95e-13 68.2
MS.gene052317.t1 MTR_4g036775 57.732 97 40 1 204 299 80 176 6.98e-33 121
MS.gene052317.t1 MTR_4g036775 52.632 57 27 0 2 58 29 85 1.19e-13 69.3
MS.gene052317.t1 MTR_5g081720 28.866 291 187 11 21 296 63 348 8.79e-33 124
MS.gene052317.t1 MTR_4g134280 33.766 231 134 8 81 296 2 228 2.17e-32 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052317.t1 AT4G16260 49.837 307 145 5 1 300 1 305 6.35e-103 303
MS.gene052317.t1 AT4G16260 49.675 308 146 5 1 301 30 335 8.15e-103 304
MS.gene052317.t1 AT3G57260 52.769 307 134 7 1 301 38 339 2.22e-102 303
MS.gene052317.t1 AT3G57260 52.769 307 134 7 1 301 54 355 2.96e-102 303
MS.gene052317.t1 AT3G57270 49.508 305 147 4 3 300 35 339 9.93e-99 294
MS.gene052317.t1 AT3G57240 49.511 307 139 7 3 301 43 341 7.58e-96 286
MS.gene052317.t1 AT4G26830 40.385 312 173 7 2 300 32 343 7.84e-72 229
MS.gene052317.t1 AT4G26830 40.385 312 173 7 2 300 36 347 1.02e-71 228
MS.gene052317.t1 AT5G56590 37.380 313 181 6 3 300 34 346 2.31e-71 229
MS.gene052317.t1 AT5G20340 40.532 301 165 9 1 291 49 345 4.40e-71 224
MS.gene052317.t1 AT1G77790 39.672 305 170 8 3 296 38 339 5.51e-71 223
MS.gene052317.t1 AT5G20330 40.333 300 165 9 1 290 40 335 5.32e-69 218
MS.gene052317.t1 AT5G20390 40.333 300 165 9 1 290 39 334 5.58e-69 218
MS.gene052317.t1 AT2G05790 38.339 313 179 7 2 300 32 344 6.99e-69 221
MS.gene052317.t1 AT4G18340 38.658 313 179 6 2 301 41 353 8.13e-68 216
MS.gene052317.t1 AT4G18340 38.658 313 179 6 2 301 41 353 9.65e-68 216
MS.gene052317.t1 AT5G55180 38.217 314 180 8 2 301 34 347 1.25e-67 218
MS.gene052317.t1 AT5G55180 38.217 314 180 8 2 301 34 347 1.28e-67 218
MS.gene052317.t1 AT2G16230 39.355 310 174 7 1 296 31 340 2.18e-67 218
MS.gene052317.t1 AT2G01630 38.636 308 173 6 5 296 33 340 9.95e-67 214
MS.gene052317.t1 AT5G42100 40.836 311 168 7 2 296 34 344 1.33e-66 214
MS.gene052317.t1 AT5G42100 40.836 311 168 7 2 296 34 344 1.66e-66 214
MS.gene052317.t1 AT2G01630 38.636 308 173 6 5 296 33 340 3.89e-66 213
MS.gene052317.t1 AT1G32860 36.943 314 183 7 2 300 36 349 4.90e-66 213
MS.gene052317.t1 AT2G01630 39.333 300 166 6 5 288 33 332 1.58e-65 213
MS.gene052317.t1 AT1G33220 39.860 286 159 8 1 276 40 322 3.23e-65 208
MS.gene052317.t1 AT2G26600 34.713 314 191 8 2 301 41 354 4.35e-65 209
MS.gene052317.t1 AT5G42720 37.942 311 178 8 1 296 32 342 8.72e-65 210
MS.gene052317.t1 AT1G30080 37.987 308 178 8 2 296 43 350 2.76e-63 204
MS.gene052317.t1 AT2G27500 35.599 309 185 8 2 296 36 344 3.74e-63 204
MS.gene052317.t1 AT4G34480 39.482 309 173 7 2 296 33 341 3.89e-63 203
MS.gene052317.t1 AT1G30080 37.987 308 178 8 2 296 43 350 5.37e-63 204
MS.gene052317.t1 AT2G27500 35.599 309 185 8 2 296 36 344 9.76e-63 203
MS.gene052317.t1 AT4G34480 39.482 309 173 7 2 296 33 341 1.99e-61 203
MS.gene052317.t1 AT3G15800 35.806 310 184 7 2 296 50 359 4.74e-61 199
MS.gene052317.t1 AT3G61810 34.076 314 188 6 3 301 63 372 6.66e-60 196
MS.gene052317.t1 AT1G77780 35.314 303 183 9 3 296 32 330 4.12e-59 193
MS.gene052317.t1 AT1G66250 37.013 308 177 7 5 296 42 348 4.72e-59 197
MS.gene052317.t1 AT5G24318 35.161 310 182 8 2 296 37 342 1.78e-58 192
MS.gene052317.t1 AT5G24318 35.161 310 182 8 2 296 37 342 2.32e-57 191
MS.gene052317.t1 AT5G24318 35.161 310 182 8 2 296 37 342 2.67e-57 191
MS.gene052317.t1 AT5G20560 36.184 304 173 9 1 290 40 336 4.02e-56 184
MS.gene052317.t1 AT2G26600 35.659 258 153 7 57 301 3 260 2.15e-55 181
MS.gene052317.t1 AT2G27500 37.549 253 145 7 57 296 14 266 1.39e-54 180
MS.gene052317.t1 AT3G07320 32.907 313 191 8 2 296 32 343 1.44e-54 184
MS.gene052317.t1 AT4G29360 34.727 311 190 6 3 300 35 345 1.03e-53 183
MS.gene052317.t1 AT4G29360 34.727 311 190 6 3 300 45 355 1.07e-53 183
MS.gene052317.t1 AT4G14080 33.228 316 191 9 3 300 50 363 5.89e-53 180
MS.gene052317.t1 AT5G58090 36.000 300 176 9 12 296 39 337 6.09e-52 177
MS.gene052317.t1 AT3G23770 33.542 319 193 8 2 301 44 362 6.43e-52 177
MS.gene052317.t1 AT1G11820 31.894 301 189 6 4 288 53 353 1.58e-51 174
MS.gene052317.t1 AT3G13560 32.143 308 193 6 5 296 36 343 5.08e-51 176
MS.gene052317.t1 AT3G13560 32.143 308 193 6 5 296 36 343 5.08e-51 176
MS.gene052317.t1 AT3G13560 32.143 308 193 6 5 296 36 343 5.08e-51 176
MS.gene052317.t1 AT3G13560 32.143 308 193 6 5 296 36 343 5.08e-51 176
MS.gene052317.t1 AT3G13560 32.143 308 193 6 5 296 36 343 5.08e-51 176
MS.gene052317.t1 AT1G11820 31.894 301 189 6 4 288 53 353 1.36e-50 174
MS.gene052317.t1 AT1G11820 31.894 301 189 6 4 288 53 353 3.23e-50 174
MS.gene052317.t1 AT2G19440 34.615 312 185 10 1 296 28 336 8.01e-50 172
MS.gene052317.t1 AT2G19440 34.615 312 185 10 1 296 28 336 8.01e-50 172
MS.gene052317.t1 AT5G58480 33.987 306 186 9 11 301 42 346 1.37e-49 171
MS.gene052317.t1 AT5G18220 32.039 309 195 7 1 296 37 343 1.51e-48 169
MS.gene052317.t1 AT4G31140 34.868 304 182 10 12 300 44 346 1.72e-47 166
MS.gene052317.t1 AT1G64760 32.476 311 193 8 1 296 32 340 7.71e-47 164
MS.gene052317.t1 AT1G64760 32.476 311 193 8 1 296 32 340 7.71e-47 164
MS.gene052317.t1 AT3G04010 31.962 316 196 9 1 300 40 352 2.01e-45 160
MS.gene052317.t1 AT3G46570 33.861 316 179 12 2 296 34 340 1.66e-44 155
MS.gene052317.t1 AT4G34480 41.667 228 120 6 82 296 3 230 5.58e-44 154
MS.gene052317.t1 AT3G24330 32.278 316 191 12 1 297 46 357 1.51e-43 155
MS.gene052317.t1 AT3G55430 32.566 304 191 10 5 296 38 339 2.55e-43 154
MS.gene052317.t1 AT2G39640 33.003 303 187 9 5 296 39 336 4.01e-43 155
MS.gene052317.t1 AT4G17180 31.894 301 184 9 12 297 42 336 1.86e-42 152
MS.gene052317.t1 AT5G64790 31.250 304 193 9 12 300 45 347 1.73e-41 150
MS.gene052317.t1 AT5G20870 31.429 315 185 12 12 300 47 356 1.80e-40 147
MS.gene052317.t1 AT5G24318 38.164 207 112 6 102 296 1 203 5.48e-40 142
MS.gene052317.t1 AT3G55780 29.139 302 191 11 18 301 58 354 1.51e-36 134

Find 72 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTATGTTGGAACCTTTA+AGG 0.089668 4.4:+34024975 None:intergenic
CAAGCCCTTCAAGCCCTTAA+AGG 0.219614 4.4:-34024989 MS.gene052317:CDS
GCTTGAAGGGCTTGTTCGTC+TGG 0.228164 4.4:+34024998 None:intergenic
CAGCGTATACTGAGTCTAAT+TGG 0.233502 4.4:+34024477 None:intergenic
GAAGCAACTGAACAACATTT+TGG 0.249494 4.4:-34024248 MS.gene052317:CDS
TTCTTTAGGAAGTCTATTAT+TGG 0.255166 4.4:+34024635 None:intergenic
ATGCCAATCAAGCTACAAAT+TGG 0.258407 4.4:-34024910 MS.gene052317:CDS
TCCACAACTTCTTGCCTTGA+TGG 0.270614 4.4:+34025064 None:intergenic
ATTGGGCATCAAAGAGGTTT+TGG 0.289119 4.4:+34024495 None:intergenic
GTGGCGCTCCATTGTTCTTT+AGG 0.292675 4.4:+34024621 None:intergenic
TTCTATGTTGGAACCTTTAA+GGG 0.304709 4.4:+34024976 None:intergenic
CATAGGGTGTCACATACTTT+TGG 0.328516 4.4:+34024885 None:intergenic
TCAATAGCTCCGGGTCTCTT+AGG 0.330851 4.4:+34024314 None:intergenic
GACCCAATTTGTAGCTTGAT+TGG 0.339546 4.4:+34024907 None:intergenic
GCATGGCAGTGGCAATGTAC+TGG 0.346712 4.4:+34024798 None:intergenic
TACTGGGCTTCATTGTCATT+AGG 0.366506 4.4:+34024815 None:intergenic
ACGCTGCTCTTGAGAAAGTT+GGG 0.379600 4.4:-34024460 MS.gene052317:CDS
GTGGATGGCCCTCGGAAGGC+GGG 0.401223 4.4:-34024406 MS.gene052317:CDS
CTTGAAGGGCTTGTTCGTCT+GGG 0.403107 4.4:+34024999 None:intergenic
TTGATCAATCACGTCAAGAA+TGG 0.432895 4.4:-34024341 MS.gene052317:CDS
TACGCTGCTCTTGAGAAAGT+TGG 0.441895 4.4:-34024461 MS.gene052317:CDS
GTTGTAAATATTGTTTAGCT+TGG 0.446199 4.4:+34024657 None:intergenic
TGGAACCTTTAAGGGCTTGA+AGG 0.446944 4.4:+34024984 None:intergenic
GGCACTATCCCCGCCTTCCG+AGG 0.456276 4.4:+34024397 None:intergenic
TGATATTTAGATGATTTGTC+AGG 0.458349 4.4:+34024215 None:intergenic
CAAGTAAGTCTCAATAGCTC+CGG 0.459135 4.4:+34024304 None:intergenic
CATGGCAGTGGCAATGTACT+GGG 0.461472 4.4:+34024799 None:intergenic
GTGGATTTATATAAGTCAAA+TGG 0.466757 4.4:-34025046 MS.gene052317:CDS
GAGAGAAGAAAGGGTTTCCT+TGG 0.473937 4.4:+34024937 None:intergenic
CAACAAGGAAACAATGATGT+TGG 0.478493 4.4:-34024521 MS.gene052317:CDS
ATTTCATCTGCAAATTTACA+AGG 0.482621 4.4:-34024758 MS.gene052317:CDS
TTCCGAGGGCCATCCACTCT+CGG 0.485934 4.4:+34024412 None:intergenic
AGCGTATACTGAGTCTAATT+GGG 0.488716 4.4:+34024478 None:intergenic
AACAAGGAAACAATGATGTT+GGG 0.493013 4.4:-34024520 MS.gene052317:CDS
TCAGTGAAAACACCATCATT+AGG 0.495646 4.4:+34024680 None:intergenic
TGATCAATCACGTCAAGAAT+GGG 0.496832 4.4:-34024340 MS.gene052317:CDS
TGCCAATCAAGCTACAAATT+GGG 0.500599 4.4:-34024909 MS.gene052317:CDS
GGAGTTTGCTATGGAATGCT+TGG 0.503294 4.4:-34025097 None:intergenic
AAAATCAAGTACATCAGTGT+TGG 0.515246 4.4:-34024851 MS.gene052317:CDS
GGCATCTGTGAGAGAAGAAA+GGG 0.523286 4.4:+34024928 None:intergenic
ACCATCAAGGCAAGAAGTTG+TGG 0.524431 4.4:-34025065 MS.gene052317:CDS
GGAACCTTTAAGGGCTTGAA+GGG 0.527762 4.4:+34024985 None:intergenic
GTCCGAGAGTGGATGGCCCT+CGG 0.531351 4.4:-34024414 MS.gene052317:CDS
TTATCTTTATAAGCGATGTA+CGG 0.538117 4.4:+34024581 None:intergenic
CGCTGCTCTTGAGAAAGTTG+GGG 0.541508 4.4:-34024459 MS.gene052317:CDS
TGGCATCTGTGAGAGAAGAA+AGG 0.541551 4.4:+34024927 None:intergenic
AATTCATATCCACCTAATGA+TGG 0.542843 4.4:-34024692 MS.gene052317:CDS
GCACTATCCCCGCCTTCCGA+GGG 0.543662 4.4:+34024398 None:intergenic
TTGAAGGGCTTGTTCGTCTG+GGG 0.546385 4.4:+34025000 None:intergenic
ATCAAGGATCAATTCTATGT+TGG 0.547280 4.4:+34024964 None:intergenic
AGTGGATGGCCCTCGGAAGG+CGG 0.547324 4.4:-34024407 MS.gene052317:CDS
AAGTAAGTCTCAATAGCTCC+GGG 0.554259 4.4:+34024305 None:intergenic
ATTGATCCTTGATGTGGCCA+AGG 0.559139 4.4:-34024954 MS.gene052317:CDS
TGGATGGCCCTCGGAAGGCG+GGG 0.562565 4.4:-34024405 MS.gene052317:CDS
GGGTTTCCTTGGCCACATCA+AGG 0.566068 4.4:+34024948 None:intergenic
GAGAGTGGATGGCCCTCGGA+AGG 0.568894 4.4:-34024410 MS.gene052317:CDS
AAATTTACAAGGACAAATCA+AGG 0.572487 4.4:-34024747 MS.gene052317:CDS
TTTGATGAAAATCAGAAGAA+TGG 0.575433 4.4:-34024272 MS.gene052317:CDS
TAGTTGTGTCCGAGAGTGGA+TGG 0.576028 4.4:-34024421 MS.gene052317:CDS
AGTCTAATTGGGCATCAAAG+AGG 0.581605 4.4:+34024489 None:intergenic
AAGATAGTTGTGTCCGAGAG+TGG 0.586296 4.4:-34024425 MS.gene052317:CDS
ATAGACTTCCTAAAGAACAA+TGG 0.595560 4.4:-34024629 MS.gene052317:CDS
GGATACACATTGGCAAGAAG+TGG 0.604193 4.4:+34024602 None:intergenic
TTGGCAATAATCTACCATCA+AGG 0.619775 4.4:-34025078 MS.gene052317:CDS
CATAGAATTGATCCTTGATG+TGG 0.622652 4.4:-34024960 MS.gene052317:CDS
GGACACAACTATCTTCACAT+TGG 0.633314 4.4:+34024433 None:intergenic
TGTGGAAGCATTATCAGTTG+AGG 0.636860 4.4:+34024376 None:intergenic
AATGGGACTCCTAAGAGACC+CGG 0.649769 4.4:-34024323 MS.gene052317:CDS
GTGAAAACACCATCATTAGG+TGG 0.653737 4.4:+34024683 None:intergenic
AGCGATGTACGGATACACAT+TGG 0.667248 4.4:+34024592 None:intergenic
GCTGCTCTTGAGAAAGTTGG+GGG 0.679213 4.4:-34024458 MS.gene052317:CDS
TATGCTCTCTTTAAACAACA+AGG 0.681281 4.4:-34024536 MS.gene052317:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATCAAATTTTGATAATATG+TGG + chr4.4:34024926-34024945 None:intergenic 20.0%
! AAATTTACAAGGACAAATCA+AGG - chr4.4:34024534-34024553 MS.gene052317:CDS 25.0%
! ATTTCATCTGCAAATTTACA+AGG - chr4.4:34024523-34024542 MS.gene052317:CDS 25.0%
! GTGGATTTATATAAGTCAAA+TGG - chr4.4:34024235-34024254 MS.gene052317:CDS 25.0%
! GTTGTAAATATTGTTTAGCT+TGG + chr4.4:34024627-34024646 None:intergenic 25.0%
! TTATCTTTATAAGCGATGTA+CGG + chr4.4:34024703-34024722 None:intergenic 25.0%
! TTTGATGAAAATCAGAAGAA+TGG - chr4.4:34025009-34025028 MS.gene052317:CDS 25.0%
!! ATTTTTACATCTTGAGCATA+GGG + chr4.4:34024415-34024434 None:intergenic 25.0%
!! TGATATTTAGATGATTTGTC+AGG + chr4.4:34025069-34025088 None:intergenic 25.0%
!! TTCTTTAGGAAGTCTATTAT+TGG + chr4.4:34024649-34024668 None:intergenic 25.0%
AAAATCAAGTACATCAGTGT+TGG - chr4.4:34024430-34024449 MS.gene052317:CDS 30.0%
AACAAGGAAACAATGATGTT+GGG - chr4.4:34024761-34024780 MS.gene052317:CDS 30.0%
AATTCATATCCACCTAATGA+TGG - chr4.4:34024589-34024608 MS.gene052317:CDS 30.0%
ATAGACTTCCTAAAGAACAA+TGG - chr4.4:34024652-34024671 MS.gene052317:CDS 30.0%
ATCAAGGATCAATTCTATGT+TGG + chr4.4:34024320-34024339 None:intergenic 30.0%
ATTCTATGTTGGAACCTTTA+AGG + chr4.4:34024309-34024328 None:intergenic 30.0%
TATGCTCTCTTTAAACAACA+AGG - chr4.4:34024745-34024764 MS.gene052317:CDS 30.0%
TTCTATGTTGGAACCTTTAA+GGG + chr4.4:34024308-34024327 None:intergenic 30.0%
! CTTCTGATTTTCATCAAACA+CGG + chr4.4:34025007-34025026 None:intergenic 30.0%
! GATTTTTACATCTTGAGCAT+AGG + chr4.4:34024416-34024435 None:intergenic 30.0%
! TGCATTTTGAATGTTTTGCA+TGG + chr4.4:34024503-34024522 None:intergenic 30.0%
AGCGTATACTGAGTCTAATT+GGG + chr4.4:34024806-34024825 None:intergenic 35.0%
ATGCCAATCAAGCTACAAAT+TGG - chr4.4:34024371-34024390 MS.gene052317:CDS 35.0%
CAACAAGGAAACAATGATGT+TGG - chr4.4:34024760-34024779 MS.gene052317:CDS 35.0%
CATAGAATTGATCCTTGATG+TGG - chr4.4:34024321-34024340 MS.gene052317:CDS 35.0%
TCAGTGAAAACACCATCATT+AGG + chr4.4:34024604-34024623 None:intergenic 35.0%
TGATCAATCACGTCAAGAAT+GGG - chr4.4:34024941-34024960 MS.gene052317:CDS 35.0%
TGCCAATCAAGCTACAAATT+GGG - chr4.4:34024372-34024391 MS.gene052317:CDS 35.0%
TTGATCAATCACGTCAAGAA+TGG - chr4.4:34024940-34024959 MS.gene052317:CDS 35.0%
TTGGCAATAATCTACCATCA+AGG - chr4.4:34024203-34024222 MS.gene052317:CDS 35.0%
! GAAGCAACTGAACAACATTT+TGG - chr4.4:34025033-34025052 MS.gene052317:CDS 35.0%
AAGTAAGTCTCAATAGCTCC+GGG + chr4.4:34024979-34024998 None:intergenic 40.0%
AGTCTAATTGGGCATCAAAG+AGG + chr4.4:34024795-34024814 None:intergenic 40.0%
CAAGTAAGTCTCAATAGCTC+CGG + chr4.4:34024980-34024999 None:intergenic 40.0%
CAGCGTATACTGAGTCTAAT+TGG + chr4.4:34024807-34024826 None:intergenic 40.0%
GACCCAATTTGTAGCTTGAT+TGG + chr4.4:34024377-34024396 None:intergenic 40.0%
GGACACAACTATCTTCACAT+TGG + chr4.4:34024851-34024870 None:intergenic 40.0%
GTGAAAACACCATCATTAGG+TGG + chr4.4:34024601-34024620 None:intergenic 40.0%
TACTGGGCTTCATTGTCATT+AGG + chr4.4:34024469-34024488 None:intergenic 40.0%
! ATTGGGCATCAAAGAGGTTT+TGG + chr4.4:34024789-34024808 None:intergenic 40.0%
! TTGAATGTTTTGCATGGCAG+TGG + chr4.4:34024497-34024516 None:intergenic 40.0%
!! CATAGGGTGTCACATACTTT+TGG + chr4.4:34024399-34024418 None:intergenic 40.0%
!! TGTGGAAGCATTATCAGTTG+AGG + chr4.4:34024908-34024927 None:intergenic 40.0%
AAGATAGTTGTGTCCGAGAG+TGG - chr4.4:34024856-34024875 MS.gene052317:CDS 45.0%
ACCATCAAGGCAAGAAGTTG+TGG - chr4.4:34024216-34024235 MS.gene052317:CDS 45.0%
ACGCTGCTCTTGAGAAAGTT+GGG - chr4.4:34024821-34024840 MS.gene052317:CDS 45.0%
AGCGATGTACGGATACACAT+TGG + chr4.4:34024692-34024711 None:intergenic 45.0%
ATTGATCCTTGATGTGGCCA+AGG - chr4.4:34024327-34024346 MS.gene052317:CDS 45.0%
GAGAGAAGAAAGGGTTTCCT+TGG + chr4.4:34024347-34024366 None:intergenic 45.0%
GGATACACATTGGCAAGAAG+TGG + chr4.4:34024682-34024701 None:intergenic 45.0%
GGCATCTGTGAGAGAAGAAA+GGG + chr4.4:34024356-34024375 None:intergenic 45.0%
TACGCTGCTCTTGAGAAAGT+TGG - chr4.4:34024820-34024839 MS.gene052317:CDS 45.0%
TCCACAACTTCTTGCCTTGA+TGG + chr4.4:34024220-34024239 None:intergenic 45.0%
TGGCATCTGTGAGAGAAGAA+AGG + chr4.4:34024357-34024376 None:intergenic 45.0%
! GGAACCTTTAAGGGCTTGAA+GGG + chr4.4:34024299-34024318 None:intergenic 45.0%
! TGGAACCTTTAAGGGCTTGA+AGG + chr4.4:34024300-34024319 None:intergenic 45.0%
AATGGGACTCCTAAGAGACC+CGG - chr4.4:34024958-34024977 MS.gene052317:CDS 50.0%
CAAGCCCTTCAAGCCCTTAA+AGG - chr4.4:34024292-34024311 MS.gene052317:CDS 50.0%
CATGGCAGTGGCAATGTACT+GGG + chr4.4:34024485-34024504 None:intergenic 50.0%
CGCTGCTCTTGAGAAAGTTG+GGG - chr4.4:34024822-34024841 MS.gene052317:CDS 50.0%
GCTGCTCTTGAGAAAGTTGG+GGG - chr4.4:34024823-34024842 MS.gene052317:CDS 50.0%
GTGGCGCTCCATTGTTCTTT+AGG + chr4.4:34024663-34024682 None:intergenic 50.0%
TAGTTGTGTCCGAGAGTGGA+TGG - chr4.4:34024860-34024879 MS.gene052317:CDS 50.0%
TCAATAGCTCCGGGTCTCTT+AGG + chr4.4:34024970-34024989 None:intergenic 50.0%
! CTTGAAGGGCTTGTTCGTCT+GGG + chr4.4:34024285-34024304 None:intergenic 50.0%
! TTGAAGGGCTTGTTCGTCTG+GGG + chr4.4:34024284-34024303 None:intergenic 50.0%
GCATGGCAGTGGCAATGTAC+TGG + chr4.4:34024486-34024505 None:intergenic 55.0%
GGGTTTCCTTGGCCACATCA+AGG + chr4.4:34024336-34024355 None:intergenic 55.0%
! GCTTGAAGGGCTTGTTCGTC+TGG + chr4.4:34024286-34024305 None:intergenic 55.0%
TTCCGAGGGCCATCCACTCT+CGG + chr4.4:34024872-34024891 None:intergenic 60.0%
AGTGGATGGCCCTCGGAAGG+CGG - chr4.4:34024874-34024893 MS.gene052317:CDS 65.0%
GAGAGTGGATGGCCCTCGGA+AGG - chr4.4:34024871-34024890 MS.gene052317:CDS 65.0%
GCACTATCCCCGCCTTCCGA+GGG + chr4.4:34024886-34024905 None:intergenic 65.0%
GTCCGAGAGTGGATGGCCCT+CGG - chr4.4:34024867-34024886 MS.gene052317:CDS 65.0%
GGCACTATCCCCGCCTTCCG+AGG + chr4.4:34024887-34024906 None:intergenic 70.0%
GTGGATGGCCCTCGGAAGGC+GGG - chr4.4:34024875-34024894 MS.gene052317:CDS 70.0%
TGGATGGCCCTCGGAAGGCG+GGG - chr4.4:34024876-34024895 MS.gene052317:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 34024199 34025104 34024199 ID=MS.gene052317
chr4.4 mRNA 34024199 34025104 34024199 ID=MS.gene052317.t1;Parent=MS.gene052317
chr4.4 exon 34024199 34025104 34024199 ID=MS.gene052317.t1.exon1;Parent=MS.gene052317.t1
chr4.4 CDS 34024199 34025104 34024199 ID=cds.MS.gene052317.t1;Parent=MS.gene052317.t1
Gene Sequence

>MS.gene052317

ATGCTTGGCAATAATCTACCATCAAGGCAAGAAGTTGTGGATTTATATAAGTCAAATGGCATTGACAAAATGCGTATATACTCCCCAGACGAACAAGCCCTTCAAGCCCTTAAAGGTTCCAACATAGAATTGATCCTTGATGTGGCCAAGGAAACCCTTTCTTCTCTCACAGATGCCAATCAAGCTACAAATTGGGTCCAAAAGTATGTGACACCCTATGCTCAAGATGTAAAAATCAAGTACATCAGTGTTGGAAATGAAATTAAACCTAATGACAATGAAGCCCAGTACATTGCCACTGCCATGCAAAACATTCAAAATGCAATTTCATCTGCAAATTTACAAGGACAAATCAAGGTCTCAACAGCAATTGACATGACTTTGATTGTTAATTCATATCCACCTAATGATGGTGTTTTCACTGACCAAGCTAAACAATATTTACAACCAATAATAGACTTCCTAAAGAACAATGGAGCGCCACTTCTTGCCAATGTGTATCCGTACATCGCTTATAAAGATAATAAACAAAGCATTTCTCTTGACTATGCTCTCTTTAAACAACAAGGAAACAATGATGTTGGGTACCAAAACCTCTTTGATGCCCAATTAGACTCAGTATACGCTGCTCTTGAGAAAGTTGGGGGTTCCAATGTGAAGATAGTTGTGTCCGAGAGTGGATGGCCCTCGGAAGGCGGGGATAGTGCCTCAACTGATAATGCTTCCACATATTATCAAAATTTGATCAATCACGTCAAGAATGGGACTCCTAAGAGACCCGGAGCTATTGAGACTTACTTGTTTGCCGTGTTTGATGAAAATCAGAAGAATGGTGAAGCAACTGAACAACATTTTGGTCTCTTTAATCCTGACAAATCATCTAAATATCAAACAAGTTTCAATTGA
Protein sequence

>MS.gene052317.t1

MLGNNLPSRQEVVDLYKSNGIDKMRIYSPDEQALQALKGSNIELILDVAKETLSSLTDANQATNWVQKYVTPYAQDVKIKYISVGNEIKPNDNEAQYIATAMQNIQNAISSANLQGQIKVSTAIDMTLIVNSYPPNDGVFTDQAKQYLQPIIDFLKNNGAPLLANVYPYIAYKDNKQSISLDYALFKQQGNNDVGYQNLFDAQLDSVYAALEKVGGSNVKIVVSESGWPSEGGDSASTDNASTYYQNLINHVKNGTPKRPGAIETYLFAVFDENQKNGEATEQHFGLFNPDKSSKYQTSFN