AlfalfaGEDB | MS.gene74300

M. sativa cultivar XinJiangDaYe / MS.gene74300

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00560.36 PF12799.10 PF13855.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74300.t1	XP_003599067.1	83.3	474	71	2	229	695	531	1003	2.50E-102	383.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74300.t1	Q5MR23	53.6	250	104	5	412	657	599	840	6.5e-19	97.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74300.t1	G7J1U6	83.3	474	71	2	229	695	531	1003	1.8e-102	383.3

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050670	MS.gene74300	0.812307	4.53E-51	-1.69E-46
MS.gene051387	MS.gene74300	0.806021	1.01E-49	-1.69E-46
MS.gene051525	MS.gene74300	0.820679	6.04E-53	-1.69E-46
MS.gene052317	MS.gene74300	0.819169	1.34E-52	-1.69E-46
MS.gene052318	MS.gene74300	0.81594	7.15E-52	-1.69E-46
MS.gene052346	MS.gene74300	0.819434	1.16E-52	-1.69E-46
MS.gene052501	MS.gene74300	0.82794	1.19E-54	-1.69E-46
MS.gene052503	MS.gene74300	0.801473	8.86E-49	-1.69E-46
MS.gene052505	MS.gene74300	0.820247	7.59E-53	-1.69E-46
MS.gene052823	MS.gene74300	0.802906	4.49E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene00013	MS.gene74300	PPI
MS.gene000654	MS.gene74300	PPI
MS.gene000656	MS.gene74300	PPI
MS.gene000659	MS.gene74300	PPI
MS.gene000667	MS.gene74300	PPI
MS.gene001273	MS.gene74300	PPI
MS.gene003372	MS.gene74300	PPI
MS.gene003767	MS.gene74300	PPI
MS.gene005385	MS.gene74300	PPI
MS.gene009835	MS.gene74300	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74300.t1	MTR_3g027330	77.135	726	109	6	1	695	304	1003	0.0	997
MS.gene74300.t1	MTR_3g027330	28.923	650	346	22	3	576	103	712	8.16e-33	136
MS.gene74300.t1	MTR_3g027330	30.717	446	268	14	139	570	95	513	1.72e-26	116
MS.gene74300.t1	MTR_3g027330	30.861	337	193	9	93	416	95	404	2.34e-17	87.0
MS.gene74300.t1	MTR_2g032560	66.712	742	169	8	1	695	284	994	0.0	872
MS.gene74300.t1	MTR_2g032560	29.935	618	341	20	3	578	85	652	1.04e-33	139
MS.gene74300.t1	MTR_2g032560	28.475	295	179	9	315	590	87	368	1.99e-12	71.2
MS.gene74300.t1	MTR_5g079980	68.706	703	159	6	1	674	351	1021	0.0	851
MS.gene74300.t1	MTR_5g079980	31.341	619	345	20	3	578	129	710	7.95e-39	155
MS.gene74300.t1	MTR_5g080000	66.667	708	172	6	1	674	250	927	0.0	831

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74300.t1	AT2G33060	39.077	737	344	17	3	676	91	785	1.13e-122	385
MS.gene74300.t1	AT2G33060	26.434	401	224	8	1	341	307	696	5.05e-20	95.5
MS.gene74300.t1	AT3G05660	38.227	722	371	12	1	661	151	858	8.44e-120	379
MS.gene74300.t1	AT3G05660	29.853	546	293	18	80	576	110	614	2.77e-27	118
MS.gene74300.t1	AT3G05660	29.455	550	267	19	107	577	84	591	4.89e-27	117
MS.gene74300.t1	AT3G05660	28.261	276	136	4	314	580	77	299	2.29e-12	70.9
MS.gene74300.t1	AT3G05660	38.168	131	75	2	480	604	103	233	1.16e-11	68.9
MS.gene74300.t1	AT2G32660	38.527	706	382	15	3	672	81	770	9.24e-118	371
MS.gene74300.t1	AT3G05650	37.150	751	384	17	1	680	135	868	1.34e-117	373
MS.gene74300.t1	AT3G05650	24.755	408	220	8	254	577	79	483	1.13e-18	91.3

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Find 150 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGAATGAGATGTTGA+AGG	0.470907	3.1:+19472670	None:intergenic
AAAAGGGAACAACATGACAT+TGG	0.627808	3.1:-19471263	MS.gene74300:CDS
AAAATAGTTAAATTTGGAAA+TGG	0.371232	3.1:+19471460	None:intergenic
AAAATCCCAGATGTGTTTGG+TGG	0.587644	3.1:-19472621	MS.gene74300:CDS
AAACCAGTGGCAATTGGATA+CGG	0.522470	3.1:-19470806	MS.gene74300:CDS
AAAGAAGGACAAGAATGAGG+AGG	0.688667	3.1:-19470679	MS.gene74300:CDS
AAATCCCAGATGTGTTTGGT+GGG	0.653753	3.1:-19472620	MS.gene74300:CDS
AAATGGCAAGGATAACAAGG+AGG	0.699207	3.1:+19472395	None:intergenic
AACTAAGATCAAGGCGTTCT+AGG	0.416800	3.1:+19471875	None:intergenic
AAGAGAAATTTGGATTTGGA+TGG	0.438178	3.1:-19470829	MS.gene74300:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACTGAATGAGATGTTGA+AGG	+	chr3.1:19470648-19470667	None:intergenic	30.0%
AAGAGAAATTTGGATTTGGA+TGG	-	chr3.1:19472486-19472505	MS.gene74300:CDS	30.0%
AGTGAAGAGAAATTTGGATT+TGG	-	chr3.1:19472482-19472501	MS.gene74300:CDS	30.0%
ATCTCAAGCAATAACTTTAG+TGG	-	chr3.1:19471882-19471901	MS.gene74300:CDS	30.0%
ATGAGGAATAGTATAAGGTA+TGG	-	chr3.1:19471969-19471988	MS.gene74300:CDS	30.0%
CTCTCATACAATTCATTTAG+CGG	-	chr3.1:19470670-19470689	MS.gene74300:CDS	30.0%
CTTAGTTACAACTTACTAAC+CGG	-	chr3.1:19471456-19471475	MS.gene74300:CDS	30.0%
CTTTCCAATAATTGTCTTGT+GGG	-	chr3.1:19472323-19472342	MS.gene74300:CDS	30.0%
GATAAATTAGCAAGACATTG+TGG	+	chr3.1:19471561-19471580	None:intergenic	30.0%
GTAAGTTGTAACTAAGATCA+AGG	+	chr3.1:19471452-19471471	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	19470625	19472712	19470625	ID=MS.gene74300
chr3.1	mRNA	19470625	19472712	19470625	ID=MS.gene74300.t1;Parent=MS.gene74300
chr3.1	exon	19470625	19472712	19470625	ID=MS.gene74300.t1.exon1;Parent=MS.gene74300.t1
chr3.1	CDS	19470625	19472712	19470625	ID=cds.MS.gene74300.t1;Parent=MS.gene74300.t1

Gene Sequence

>MS.gene74300

ATGCCATCCTCACTTTCAAACCTTCAACATCTCATTCAGTTTTATCTCTCATACAATTCATTTAGCGGTAAAATCCCAGATGTGTTTGGTGGGATGACAAAACTACAAGAACTCTACTTATATTCTAACAATTTAGAAGGCGAAATTCCTTCTTCATTATTCAACTCGTCTCAGCTTGTTGAATTAGATTGTTCTGATAACAAATTAGAGGGTCCTCTGCCCAACCAAATTATAAGGTTCCAAAAGCTAACTGATTTGAGATTAAATGACAACTTACTAAATGGTACAATTCCTCCCTCCTTGTTATCCTTGCCATTTTTGCTAGAATTAGATTTATCAAATAATCGACTTACAGGGCATATCAGCGCAATCTCATCATATTCCTTAGAGTCGCTATATCTAAGTAACAACAGGCTACAAGGCAATATTCCAAAATCCATTTTCAACCTTGCAAACCTAAGTGATCTATCCCTATCATCAAACAACTTGAGTGGTCATGTTGATTTTCATCTCTTCTCAAACCTTCCAGGCCTAAGTTTTCTAGATCTATCATCAAACAACTTGAGTGGTGTTGTCAATTTCCAAACCATTTCCAAACTTCAAAATTTGTATTTTCTGTCCCTTTCAGATAATAGCCAATTATCAATAAACTTTGAATCCAGTGTCAATTACAATATTTCTAGTCTATTTGAATTGGGTTTGTCTTCTTTGAGTTTGACTGAATTTCCTAATTTGTCAGAAAAATTCCCATTGTTGGGATATCTTGATCTGTCTAATAACAAAATTAGTGGAAGTGTGCCCAATCTTGATCTTGACCTAGAACGCCTTGATCTTAGTTACAACTTACTAACCGGTGACATCTCTTTGTCTATTTGCAATGCAAGTGGCCTTTCATTTCTCAGTTTGGCATACAATCAATTGACAGGCACCATTCCACAATGTCTTGCTAATTTATCATCCCTTGAAGTTTTGGATCTACAAATGAACAAATTTCATGGCCATTTGCCAAGTAACAGTGCACTTGAGAGTCTGAATCTCTATGGCAACCAATTAGAAGGTCATATTCCTAAATCCCTGTCTCTTTGTAAAAGGTTGAAGTTTCTAAACCTTGGCAGCAACAAAATAGAAGACAATTTTCCACATTGGCTTCAAACCTTACAATATTTGAAAGTGTTGGTTTTGCGAGACAATAAGCTGCATGGTATCATTGTTAATCCAAAGATTAAGCATCCATTTCCAAATTTAACTATTTTTGATATCTCAAGCAATAACTTTAGTGGTCCTCTACCAAAAGCCTATTTCAAAAAGTTTGAAGCCATGATGAATGTTACTGAGTTGGAATATATGAGGAATAGTATAAGGTATGGGCCCTTCTCAGCCTTCGGCCAACAATTTGCATCCTACTATGATTCTGTGATTGTGGCGACAAAAGGGAACAACATGACATTGGTGAAAATTCCAAACATCTTTGTAATTATTGATTTGTCAAGAAACAAATTTGAAGGAGACATTCCAAACGTTATTGGTGAGCTTCATGCAATCATAGGGCTTAACCTTTCCCATAACAGACTTACAGGTCATATTCCAAAATCCATAGGAAACTTGACATACTTGGAATCTTTGGATCTCTCCTCAAATTTGTTAACCGGTGCGATTCCTTTGGAATTAACCAATCTTAACAGTCTTGAAGTCTTGAATCTTTCCAATAATTGTCTTGTGGGAGAAATACCTCAAGGAAATAAATTCAATACTTTTACAAATGATTCATATGAAGGAAACTTGGAGTTATGTGGATTACCCTTGTCAAAGATGTGTGGACCTGAACAACATTCTGCACCTTCAGCCAACAACTTTTGCAGTGAAGAGAAATTTGGATTTGGATGGAAACCAGTGGCAATTGGATACGGATGTGGATTTGTGATTGGAATAGGCATTGGATATTATATGTTTTTAATTGGAAAGCCGAGATGGCTTGTCATGATATTTGGTGGTCAGCCTAAGAGGAGAGTGAAAAGAAGGACAAGAATGAGGAGGAACCAAAGTTCAATCACGAATCAGAATCAGAATCAGATGGTGCAAATGTCTTAA

Protein sequence

>MS.gene74300.t1

MPSSLSNLQHLIQFYLSYNSFSGKIPDVFGGMTKLQELYLYSNNLEGEIPSSLFNSSQLVELDCSDNKLEGPLPNQIIRFQKLTDLRLNDNLLNGTIPPSLLSLPFLLELDLSNNRLTGHISAISSYSLESLYLSNNRLQGNIPKSIFNLANLSDLSLSSNNLSGHVDFHLFSNLPGLSFLDLSSNNLSGVVNFQTISKLQNLYFLSLSDNSQLSINFESSVNYNISSLFELGLSSLSLTEFPNLSEKFPLLGYLDLSNNKISGSVPNLDLDLERLDLSYNLLTGDISLSICNASGLSFLSLAYNQLTGTIPQCLANLSSLEVLDLQMNKFHGHLPSNSALESLNLYGNQLEGHIPKSLSLCKRLKFLNLGSNKIEDNFPHWLQTLQYLKVLVLRDNKLHGIIVNPKIKHPFPNLTIFDISSNNFSGPLPKAYFKKFEAMMNVTELEYMRNSIRYGPFSAFGQQFASYYDSVIVATKGNNMTLVKIPNIFVIIDLSRNKFEGDIPNVIGELHAIIGLNLSHNRLTGHIPKSIGNLTYLESLDLSSNLLTGAIPLELTNLNSLEVLNLSNNCLVGEIPQGNKFNTFTNDSYEGNLELCGLPLSKMCGPEQHSAPSANNFCSEEKFGFGWKPVAIGYGCGFVIGIGIGYYMFLIGKPRWLVMIFGGQPKRRVKRRTRMRRNQSSITNQNQNQMVQMS