Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052668.t1 | XP_003630930.1 | 100 | 164 | 0 | 0 | 1 | 164 | 1 | 164 | 3.90E-91 | 344 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052668.t1 | Q9ZU95 | 68.6 | 159 | 49 | 1 | 1 | 159 | 6 | 163 | 8.9e-60 | 231.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052668.t1 | G7LAJ8 | 100.0 | 164 | 0 | 0 | 1 | 164 | 1 | 164 | 2.8e-91 | 344.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049160 | MS.gene052668 | 0.852875 | 3.46E-61 | -1.69E-46 |
| MS.gene052668 | MS.gene62693 | 0.915295 | 6.98E-85 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052668.t1 | MTR_8g105140 | 100.000 | 164 | 0 | 0 | 1 | 164 | 1 | 164 | 6.55e-121 | 337 |
| MS.gene052668.t1 | MTR_1g032290 | 81.646 | 158 | 29 | 0 | 1 | 158 | 1 | 158 | 2.45e-96 | 275 |
| MS.gene052668.t1 | MTR_3g065330 | 63.804 | 163 | 56 | 2 | 1 | 161 | 10 | 171 | 2.15e-71 | 213 |
| MS.gene052668.t1 | MTR_5g078020 | 61.111 | 162 | 52 | 4 | 1 | 157 | 10 | 165 | 7.01e-64 | 194 |
| MS.gene052668.t1 | MTR_6g070920 | 59.006 | 161 | 62 | 3 | 1 | 160 | 72 | 229 | 3.24e-63 | 194 |
| MS.gene052668.t1 | MTR_3g065330 | 62.963 | 135 | 47 | 2 | 1 | 133 | 32 | 165 | 2.45e-55 | 172 |
| MS.gene052668.t1 | MTR_6g070920 | 57.971 | 138 | 54 | 3 | 1 | 137 | 72 | 206 | 1.27e-51 | 164 |
| MS.gene052668.t1 | MTR_8g039690 | 49.057 | 159 | 71 | 5 | 1 | 151 | 1 | 157 | 1.88e-42 | 139 |
| MS.gene052668.t1 | MTR_7g084380 | 49.375 | 160 | 70 | 5 | 1 | 150 | 1 | 159 | 2.42e-37 | 128 |
| MS.gene052668.t1 | MTR_7g084380 | 51.111 | 135 | 56 | 4 | 26 | 150 | 21 | 155 | 3.41e-32 | 114 |
| MS.gene052668.t1 | MTR_6g070920 | 59.783 | 92 | 33 | 3 | 1 | 91 | 72 | 160 | 1.67e-30 | 108 |
| MS.gene052668.t1 | MTR_1g078390 | 44.615 | 65 | 34 | 2 | 1 | 64 | 1 | 64 | 3.72e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052668.t1 | AT1G14860 | 69.427 | 157 | 47 | 1 | 1 | 157 | 1 | 156 | 1.96e-77 | 228 |
| MS.gene052668.t1 | AT2G01670 | 68.553 | 159 | 49 | 1 | 1 | 159 | 6 | 163 | 2.72e-77 | 228 |
| MS.gene052668.t1 | AT1G18300 | 54.839 | 155 | 69 | 1 | 1 | 154 | 40 | 194 | 8.99e-63 | 192 |
| MS.gene052668.t1 | AT1G73540 | 52.469 | 162 | 72 | 2 | 1 | 161 | 41 | 198 | 2.10e-59 | 183 |
| MS.gene052668.t1 | AT1G12880 | 49.390 | 164 | 69 | 4 | 1 | 151 | 1 | 163 | 1.02e-41 | 138 |
| MS.gene052668.t1 | AT3G26690 | 43.820 | 178 | 85 | 4 | 1 | 164 | 1 | 177 | 1.35e-40 | 135 |
| MS.gene052668.t1 | AT3G26690 | 43.820 | 178 | 85 | 4 | 1 | 164 | 1 | 177 | 1.35e-40 | 135 |
| MS.gene052668.t1 | AT3G12600 | 47.170 | 159 | 74 | 4 | 1 | 150 | 1 | 158 | 2.01e-33 | 117 |
| MS.gene052668.t1 | AT3G12600 | 46.939 | 147 | 68 | 4 | 13 | 150 | 4 | 149 | 7.12e-30 | 107 |
Find 40 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGTCTTCTTTATATCTATA+AGG | 0.288768 | 4.1:-1805057 | None:intergenic |
| AAAATGTTAGGAGAAGAATA+TGG | 0.319122 | 4.1:+1805560 | MS.gene052668:CDS |
| TTCATATGACAGTGCGAATT+GGG | 0.322626 | 4.1:+1805442 | MS.gene052668:intron |
| AAGAAGACATAGATGGTAAT+AGG | 0.347424 | 4.1:+1805070 | MS.gene052668:CDS |
| GAAGGAAGCATTAGATATAT+TGG | 0.347737 | 4.1:+1805745 | MS.gene052668:CDS |
| ATTCATATGACAGTGCGAAT+TGG | 0.395799 | 4.1:+1805441 | MS.gene052668:intron |
| ATGGGTGGCCGCCAAGTTGT+AGG | 0.417911 | 4.1:+1804682 | MS.gene052668:CDS |
| GTTGAAAGATATGGTTTGTT+TGG | 0.419854 | 4.1:+1804621 | None:intergenic |
| CTGTGACTCCTGCTTCTTCA+AGG | 0.425939 | 4.1:-1805319 | None:intergenic |
| GAGAAGTTTGTCAGCATTGG+TGG | 0.451878 | 4.1:+1805720 | MS.gene052668:CDS |
| TGGTAATAGGAGCAACGAAT+TGG | 0.454436 | 4.1:+1805083 | MS.gene052668:CDS |
| GTTTGTTTGGTATCTCGCTC+TGG | 0.459609 | 4.1:+1804634 | MS.gene052668:CDS |
| CATGTTCCCTTTGTTTGTGA+AGG | 0.465572 | 4.1:+1805513 | MS.gene052668:CDS |
| TTGTCAGCATTGGTGGATGA+AGG | 0.468481 | 4.1:+1805727 | MS.gene052668:CDS |
| TGTGACTCCTGCTTCTTCAA+GGG | 0.476266 | 4.1:-1805318 | None:intergenic |
| CAAGAGAAGTTTGTCAGCAT+TGG | 0.482593 | 4.1:+1805717 | MS.gene052668:CDS |
| AGATATAAAGAAGACATAGA+TGG | 0.486650 | 4.1:+1805063 | MS.gene052668:CDS |
| AATTTCATTAGCAAAAGATA+TGG | 0.486910 | 4.1:+1805472 | MS.gene052668:CDS |
| ATTGATCTCAACAGGGAGGA+TGG | 0.489262 | 4.1:+1805255 | MS.gene052668:intron |
| GTTTGGTATCTCGCTCTGGA+AGG | 0.507086 | 4.1:+1804638 | MS.gene052668:CDS |
| CTTGAAGAAGCAGGAGTCAC+AGG | 0.513097 | 4.1:+1805320 | MS.gene052668:CDS |
| AAATGTTAGGAGAAGAATAT+GGG | 0.514195 | 4.1:+1805561 | MS.gene052668:CDS |
| GACAGTGCGAATTGGGGCAG+TGG | 0.525400 | 4.1:+1805449 | MS.gene052668:intron |
| TTTGGTATCTCGCTCTGGAA+GGG | 0.527970 | 4.1:+1804639 | MS.gene052668:CDS |
| AGTTGCTCCTTCACAAACAA+AGG | 0.531029 | 4.1:-1805520 | None:intergenic |
| TGGGTGGCCGCCAAGTTGTA+GGG | 0.544829 | 4.1:+1804683 | MS.gene052668:CDS |
| AGAGAATCCCTTGAAGAAGC+AGG | 0.547892 | 4.1:+1805311 | MS.gene052668:CDS |
| GTACTACTCACCCTACAACT+TGG | 0.553526 | 4.1:-1804693 | None:intergenic |
| AGATATGGCATATACTATGA+AGG | 0.554822 | 4.1:+1805487 | MS.gene052668:CDS |
| TTGATCTCAACAGGGAGGAT+GGG | 0.565610 | 4.1:+1805256 | MS.gene052668:intron |
| GTTGCTCCTTCACAAACAAA+GGG | 0.573725 | 4.1:-1805519 | None:intergenic |
| CAACGAATTGGAAGTACTAG+TGG | 0.598559 | 4.1:+1805095 | MS.gene052668:CDS |
| TCAGAGACTAATGTTTCCCA+AGG | 0.618632 | 4.1:+1805134 | MS.gene052668:CDS |
| CTACTCACCCTACAACTTGG+CGG | 0.620694 | 4.1:-1804690 | None:intergenic |
| TCATATGACAGTGCGAATTG+GGG | 0.622366 | 4.1:+1805443 | MS.gene052668:intron |
| TGAAGGAGCAACTTGATCAG+TGG | 0.636553 | 4.1:+1805530 | MS.gene052668:CDS |
| AGGAGTCACAGGATTAGTCG+AGG | 0.665268 | 4.1:+1805331 | MS.gene052668:CDS |
| CAGAGATATAACAACATGGG+TGG | 0.667388 | 4.1:+1804667 | MS.gene052668:CDS |
| TTGCAGAGATATAACAACAT+GGG | 0.682172 | 4.1:+1804664 | MS.gene052668:CDS |
| GTTGCAGAGATATAACAACA+TGG | 0.704593 | 4.1:+1804663 | MS.gene052668:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATTAATATTCCAATTAA+GGG | - | chr4.1:1805218-1805237 | None:intergenic | 10.0% |
| !! | GTTAATTAATATTCCAATTA+AGG | - | chr4.1:1805219-1805238 | None:intergenic | 15.0% |
| !! | TAATTAATATTCCAATTAAG+GGG | - | chr4.1:1805217-1805236 | None:intergenic | 15.0% |
| !! | AAAATGAAGTGAATAGTATT+TGG | - | chr4.1:1804739-1804758 | None:intergenic | 20.0% |
| !! | AATTAATATTCCAATTAAGG+GGG | - | chr4.1:1805216-1805235 | None:intergenic | 20.0% |
| !! | AATTTCATTAGCAAAAGATA+TGG | + | chr4.1:1805472-1805491 | MS.gene052668:CDS | 20.0% |
| !! | ATGTCTTCTTTATATCTATA+AGG | - | chr4.1:1805060-1805079 | None:intergenic | 20.0% |
| !! | TGAAATGAATTTGAAAAATG+TGG | - | chr4.1:1805163-1805182 | None:intergenic | 20.0% |
| !! | TTGAGATCAATCAAATATAT+AGG | - | chr4.1:1805246-1805265 | None:intergenic | 20.0% |
| ! | AAAATGTTAGGAGAAGAATA+TGG | + | chr4.1:1805560-1805579 | MS.gene052668:CDS | 25.0% |
| ! | AAATGTTAGGAGAAGAATAT+GGG | + | chr4.1:1805561-1805580 | MS.gene052668:CDS | 25.0% |
| ! | AGATATAAAGAAGACATAGA+TGG | + | chr4.1:1805063-1805082 | MS.gene052668:CDS | 25.0% |
| ! | ATATTTGATTGATCTCAACA+GGG | + | chr4.1:1805248-1805267 | MS.gene052668:intron | 25.0% |
| ! | TATATTTGATTGATCTCAAC+AGG | + | chr4.1:1805247-1805266 | MS.gene052668:intron | 25.0% |
| ! | TTTCACGTTGCAAAAATATT+TGG | + | chr4.1:1804757-1804776 | MS.gene052668:intron | 25.0% |
| AAGAAGACATAGATGGTAAT+AGG | + | chr4.1:1805070-1805089 | MS.gene052668:CDS | 30.0% | |
| AATATTTGGTAGGTGACATA+TGG | + | chr4.1:1804771-1804790 | MS.gene052668:intron | 30.0% | |
| ACGTTGCAAAAATATTTGGT+AGG | + | chr4.1:1804761-1804780 | MS.gene052668:intron | 30.0% | |
| AGATATGGCATATACTATGA+AGG | + | chr4.1:1805487-1805506 | MS.gene052668:CDS | 30.0% | |
| TTGAAAAATGTGGATACCTT+GGG | - | chr4.1:1805153-1805172 | None:intergenic | 30.0% | |
| TTGCAGAGATATAACAACAT+GGG | + | chr4.1:1804664-1804683 | MS.gene052668:CDS | 30.0% | |
| TTTGAAAAATGTGGATACCT+TGG | - | chr4.1:1805154-1805173 | None:intergenic | 30.0% | |
| !! | GAAGGAAGCATTAGATATAT+TGG | + | chr4.1:1805745-1805764 | MS.gene052668:CDS | 30.0% |
| ATTCATATGACAGTGCGAAT+TGG | + | chr4.1:1805441-1805460 | MS.gene052668:intron | 35.0% | |
| GTTGCAGAGATATAACAACA+TGG | + | chr4.1:1804663-1804682 | MS.gene052668:CDS | 35.0% | |
| TTCATATGACAGTGCGAATT+GGG | + | chr4.1:1805442-1805461 | MS.gene052668:intron | 35.0% | |
| ! | CTTCTCCTAACATTTTTCTC+AGG | - | chr4.1:1805556-1805575 | None:intergenic | 35.0% |
| !! | ACTTGCTCATTAGTTGCTTT+TGG | + | chr4.1:1805188-1805207 | MS.gene052668:intron | 35.0% |
| !! | GGATTAGTCGAGGTATTAAT+TGG | + | chr4.1:1805341-1805360 | MS.gene052668:intron | 35.0% |
| AGTGGCCTGAGAAAAATGTT+AGG | + | chr4.1:1805548-1805567 | MS.gene052668:CDS | 40.0% | |
| AGTTGCTCCTTCACAAACAA+AGG | - | chr4.1:1805523-1805542 | None:intergenic | 40.0% | |
| CAAGAGAAGTTTGTCAGCAT+TGG | + | chr4.1:1805717-1805736 | MS.gene052668:CDS | 40.0% | |
| CAGAGATATAACAACATGGG+TGG | + | chr4.1:1804667-1804686 | MS.gene052668:CDS | 40.0% | |
| GTTGCTCCTTCACAAACAAA+GGG | - | chr4.1:1805522-1805541 | None:intergenic | 40.0% | |
| TCAGAGACTAATGTTTCCCA+AGG | + | chr4.1:1805134-1805153 | MS.gene052668:CDS | 40.0% | |
| TCATATGACAGTGCGAATTG+GGG | + | chr4.1:1805443-1805462 | MS.gene052668:intron | 40.0% | |
| TGGTAATAGGAGCAACGAAT+TGG | + | chr4.1:1805083-1805102 | MS.gene052668:CDS | 40.0% | |
| TTTGATTGATCTCAACAGGG+AGG | + | chr4.1:1805251-1805270 | MS.gene052668:intron | 40.0% | |
| ! | CATGTTCCCTTTGTTTGTGA+AGG | + | chr4.1:1805513-1805532 | MS.gene052668:CDS | 40.0% |
| !! | CAACGAATTGGAAGTACTAG+TGG | + | chr4.1:1805095-1805114 | MS.gene052668:CDS | 40.0% |
| AGAGAATCCCTTGAAGAAGC+AGG | + | chr4.1:1805311-1805330 | MS.gene052668:CDS | 45.0% | |
| ATTGATCTCAACAGGGAGGA+TGG | + | chr4.1:1805255-1805274 | MS.gene052668:intron | 45.0% | |
| GTACTACTCACCCTACAACT+TGG | - | chr4.1:1804696-1804715 | None:intergenic | 45.0% | |
| GTTTGTTTGGTATCTCGCTC+TGG | + | chr4.1:1804634-1804653 | MS.gene052668:CDS | 45.0% | |
| TGAAGGAGCAACTTGATCAG+TGG | + | chr4.1:1805530-1805549 | MS.gene052668:CDS | 45.0% | |
| TGTGACTCCTGCTTCTTCAA+GGG | - | chr4.1:1805321-1805340 | None:intergenic | 45.0% | |
| TTGATCTCAACAGGGAGGAT+GGG | + | chr4.1:1805256-1805275 | MS.gene052668:intron | 45.0% | |
| ! | GAGAAGTTTGTCAGCATTGG+TGG | + | chr4.1:1805720-1805739 | MS.gene052668:CDS | 45.0% |
| !! | TTGTCAGCATTGGTGGATGA+AGG | + | chr4.1:1805727-1805746 | MS.gene052668:CDS | 45.0% |
| !! | TTTGGTATCTCGCTCTGGAA+GGG | + | chr4.1:1804639-1804658 | MS.gene052668:CDS | 45.0% |
| !!! | GCTTTTGGAACCCCCTTAAT+TGG | + | chr4.1:1805203-1805222 | MS.gene052668:intron | 45.0% |
| CTACTCACCCTACAACTTGG+CGG | - | chr4.1:1804693-1804712 | None:intergenic | 50.0% | |
| CTGTGACTCCTGCTTCTTCA+AGG | - | chr4.1:1805322-1805341 | None:intergenic | 50.0% | |
| CTTGAAGAAGCAGGAGTCAC+AGG | + | chr4.1:1805320-1805339 | MS.gene052668:CDS | 50.0% | |
| ! | GTTTGGTATCTCGCTCTGGA+AGG | + | chr4.1:1804638-1804657 | MS.gene052668:CDS | 50.0% |
| !! | AGGAGTCACAGGATTAGTCG+AGG | + | chr4.1:1805331-1805350 | MS.gene052668:CDS | 50.0% |
| ATGGGTGGCCGCCAAGTTGT+AGG | + | chr4.1:1804682-1804701 | MS.gene052668:CDS | 60.0% | |
| GACAGTGCGAATTGGGGCAG+TGG | + | chr4.1:1805449-1805468 | MS.gene052668:intron | 60.0% | |
| TGGGTGGCCGCCAAGTTGTA+GGG | + | chr4.1:1804683-1804702 | MS.gene052668:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 1804631 | 1805802 | 1804631 | ID=MS.gene052668 |
| chr4.1 | mRNA | 1804631 | 1805802 | 1804631 | ID=MS.gene052668.t1;Parent=MS.gene052668 |
| chr4.1 | exon | 1804631 | 1804704 | 1804631 | ID=MS.gene052668.t1.exon1;Parent=MS.gene052668.t1 |
| chr4.1 | CDS | 1804631 | 1804704 | 1804631 | ID=cds.MS.gene052668.t1;Parent=MS.gene052668.t1 |
| chr4.1 | exon | 1805050 | 1805155 | 1805050 | ID=MS.gene052668.t1.exon2;Parent=MS.gene052668.t1 |
| chr4.1 | CDS | 1805050 | 1805155 | 1805050 | ID=cds.MS.gene052668.t1;Parent=MS.gene052668.t1 |
| chr4.1 | exon | 1805269 | 1805352 | 1805269 | ID=MS.gene052668.t1.exon3;Parent=MS.gene052668.t1 |
| chr4.1 | CDS | 1805269 | 1805352 | 1805269 | ID=cds.MS.gene052668.t1;Parent=MS.gene052668.t1 |
| chr4.1 | exon | 1805454 | 1805582 | 1805454 | ID=MS.gene052668.t1.exon4;Parent=MS.gene052668.t1 |
| chr4.1 | CDS | 1805454 | 1805582 | 1805454 | ID=cds.MS.gene052668.t1;Parent=MS.gene052668.t1 |
| chr4.1 | exon | 1805701 | 1805802 | 1805701 | ID=MS.gene052668.t1.exon5;Parent=MS.gene052668.t1 |
| chr4.1 | CDS | 1805701 | 1805802 | 1805701 | ID=cds.MS.gene052668.t1;Parent=MS.gene052668.t1 |
| Gene Sequence |
| Protein sequence |
