Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62693.t1 | XP_003630930.1 | 100 | 164 | 0 | 0 | 1 | 164 | 1 | 164 | 3.90E-91 | 344 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62693.t1 | Q9ZU95 | 68.6 | 159 | 49 | 1 | 1 | 159 | 6 | 163 | 8.9e-60 | 231.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62693.t1 | G7LAJ8 | 100.0 | 164 | 0 | 0 | 1 | 164 | 1 | 164 | 2.8e-91 | 344.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049160 | MS.gene62693 | 0.871618 | 5.96E-67 | -1.69E-46 |
| MS.gene052668 | MS.gene62693 | 0.915295 | 6.98E-85 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62693.t1 | MTR_8g105140 | 100.000 | 164 | 0 | 0 | 1 | 164 | 1 | 164 | 6.55e-121 | 337 |
| MS.gene62693.t1 | MTR_1g032290 | 81.646 | 158 | 29 | 0 | 1 | 158 | 1 | 158 | 2.45e-96 | 275 |
| MS.gene62693.t1 | MTR_3g065330 | 63.804 | 163 | 56 | 2 | 1 | 161 | 10 | 171 | 2.15e-71 | 213 |
| MS.gene62693.t1 | MTR_5g078020 | 61.111 | 162 | 52 | 4 | 1 | 157 | 10 | 165 | 7.01e-64 | 194 |
| MS.gene62693.t1 | MTR_6g070920 | 59.006 | 161 | 62 | 3 | 1 | 160 | 72 | 229 | 3.24e-63 | 194 |
| MS.gene62693.t1 | MTR_3g065330 | 62.963 | 135 | 47 | 2 | 1 | 133 | 32 | 165 | 2.45e-55 | 172 |
| MS.gene62693.t1 | MTR_6g070920 | 57.971 | 138 | 54 | 3 | 1 | 137 | 72 | 206 | 1.27e-51 | 164 |
| MS.gene62693.t1 | MTR_8g039690 | 49.057 | 159 | 71 | 5 | 1 | 151 | 1 | 157 | 1.88e-42 | 139 |
| MS.gene62693.t1 | MTR_7g084380 | 49.375 | 160 | 70 | 5 | 1 | 150 | 1 | 159 | 2.42e-37 | 128 |
| MS.gene62693.t1 | MTR_7g084380 | 51.111 | 135 | 56 | 4 | 26 | 150 | 21 | 155 | 3.41e-32 | 114 |
| MS.gene62693.t1 | MTR_6g070920 | 59.783 | 92 | 33 | 3 | 1 | 91 | 72 | 160 | 1.67e-30 | 108 |
| MS.gene62693.t1 | MTR_1g078390 | 44.615 | 65 | 34 | 2 | 1 | 64 | 1 | 64 | 3.72e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62693.t1 | AT1G14860 | 69.427 | 157 | 47 | 1 | 1 | 157 | 1 | 156 | 1.96e-77 | 228 |
| MS.gene62693.t1 | AT2G01670 | 68.553 | 159 | 49 | 1 | 1 | 159 | 6 | 163 | 2.72e-77 | 228 |
| MS.gene62693.t1 | AT1G18300 | 54.839 | 155 | 69 | 1 | 1 | 154 | 40 | 194 | 8.99e-63 | 192 |
| MS.gene62693.t1 | AT1G73540 | 52.469 | 162 | 72 | 2 | 1 | 161 | 41 | 198 | 2.10e-59 | 183 |
| MS.gene62693.t1 | AT1G12880 | 49.390 | 164 | 69 | 4 | 1 | 151 | 1 | 163 | 1.02e-41 | 138 |
| MS.gene62693.t1 | AT3G26690 | 43.820 | 178 | 85 | 4 | 1 | 164 | 1 | 177 | 1.35e-40 | 135 |
| MS.gene62693.t1 | AT3G26690 | 43.820 | 178 | 85 | 4 | 1 | 164 | 1 | 177 | 1.35e-40 | 135 |
| MS.gene62693.t1 | AT3G12600 | 47.170 | 159 | 74 | 4 | 1 | 150 | 1 | 158 | 2.01e-33 | 117 |
| MS.gene62693.t1 | AT3G12600 | 46.939 | 147 | 68 | 4 | 13 | 150 | 4 | 149 | 7.12e-30 | 107 |
Find 40 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGTCTTCTTTATATCTATA+AGG | 0.288768 | 4.2:-1567001 | None:intergenic |
| AAAATGTTAGGAGAAGAATA+TGG | 0.319122 | 4.2:+1567505 | MS.gene62693:CDS |
| TTCATATGACAGTGCGAATT+GGG | 0.322626 | 4.2:+1567387 | MS.gene62693:intron |
| AAGAAGACATAGATGGTAAT+AGG | 0.347424 | 4.2:+1567014 | MS.gene62693:CDS |
| GAAGGAAGCATTAGATATAT+TGG | 0.347737 | 4.2:+1567690 | MS.gene62693:CDS |
| ATTCATATGACAGTGCGAAT+TGG | 0.395799 | 4.2:+1567386 | MS.gene62693:intron |
| ATGGGTGGCCGCCAAGTTGT+AGG | 0.417911 | 4.2:+1566626 | MS.gene62693:CDS |
| GTTGAAAGATATGGTTTGTT+TGG | 0.419854 | 4.2:+1566565 | None:intergenic |
| CTGTGACTCCTGCTTCTTCA+AGG | 0.425939 | 4.2:-1567263 | None:intergenic |
| GAGAAGTTTGTCAGCATTGG+TGG | 0.451878 | 4.2:+1567665 | MS.gene62693:CDS |
| TGGTAATAGGAGCAACGAAT+TGG | 0.454436 | 4.2:+1567027 | MS.gene62693:CDS |
| GTTTGTTTGGTATCTCGCTC+TGG | 0.459609 | 4.2:+1566578 | MS.gene62693:CDS |
| CATGTTCCCTTTGTTTGTGA+AGG | 0.465572 | 4.2:+1567458 | MS.gene62693:CDS |
| TTGTCAGCATTGGTGGATGA+AGG | 0.468481 | 4.2:+1567672 | MS.gene62693:CDS |
| TGTGACTCCTGCTTCTTCAA+GGG | 0.476266 | 4.2:-1567262 | None:intergenic |
| CAAGAGAAGTTTGTCAGCAT+TGG | 0.482593 | 4.2:+1567662 | MS.gene62693:CDS |
| AGATATAAAGAAGACATAGA+TGG | 0.486650 | 4.2:+1567007 | MS.gene62693:CDS |
| AATTTCATTAGCAAAAGATA+TGG | 0.486910 | 4.2:+1567417 | MS.gene62693:CDS |
| ATTGATCTCAACAGGGAGGA+TGG | 0.489262 | 4.2:+1567199 | MS.gene62693:intron |
| GTTTGGTATCTCGCTCTGGA+AGG | 0.507086 | 4.2:+1566582 | MS.gene62693:CDS |
| CTTGAAGAAGCAGGAGTCAC+AGG | 0.513097 | 4.2:+1567264 | MS.gene62693:CDS |
| AAATGTTAGGAGAAGAATAT+GGG | 0.514195 | 4.2:+1567506 | MS.gene62693:CDS |
| GACAGTGCGAATTGGGGCAG+TGG | 0.525400 | 4.2:+1567394 | MS.gene62693:intron |
| TTTGGTATCTCGCTCTGGAA+GGG | 0.527970 | 4.2:+1566583 | MS.gene62693:CDS |
| AGTTGCTCCTTCACAAACAA+AGG | 0.531029 | 4.2:-1567465 | None:intergenic |
| TGGGTGGCCGCCAAGTTGTA+GGG | 0.544829 | 4.2:+1566627 | MS.gene62693:CDS |
| AGAGAATCCCTTGAAGAAGC+AGG | 0.547892 | 4.2:+1567255 | MS.gene62693:CDS |
| GTACTACTCACCCTACAACT+TGG | 0.553526 | 4.2:-1566637 | None:intergenic |
| AGATATGGCATATACTATGA+AGG | 0.554822 | 4.2:+1567432 | MS.gene62693:CDS |
| TTGATCTCAACAGGGAGGAT+GGG | 0.565610 | 4.2:+1567200 | MS.gene62693:intron |
| GTTGCTCCTTCACAAACAAA+GGG | 0.573725 | 4.2:-1567464 | None:intergenic |
| CAACGAATTGGAAGTACTAG+TGG | 0.598559 | 4.2:+1567039 | MS.gene62693:CDS |
| TCAGAGACTAATGTTTCCCA+AGG | 0.618632 | 4.2:+1567078 | MS.gene62693:CDS |
| CTACTCACCCTACAACTTGG+CGG | 0.620694 | 4.2:-1566634 | None:intergenic |
| TCATATGACAGTGCGAATTG+GGG | 0.622366 | 4.2:+1567388 | MS.gene62693:intron |
| AGGAGTCACAGGATTAGTTG+AGG | 0.634343 | 4.2:+1567275 | MS.gene62693:CDS |
| TGAAGGAGCAACTTGATCAG+TGG | 0.636553 | 4.2:+1567475 | MS.gene62693:CDS |
| CAGAGATATAACAACATGGG+TGG | 0.667388 | 4.2:+1566611 | MS.gene62693:CDS |
| TTGCAGAGATATAACAACAT+GGG | 0.682172 | 4.2:+1566608 | MS.gene62693:CDS |
| GTTGCAGAGATATAACAACA+TGG | 0.704593 | 4.2:+1566607 | MS.gene62693:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATTAATATTCCAATTAA+GGG | - | chr4.2:1567162-1567181 | None:intergenic | 10.0% |
| !! | GTTAATTAATATTCCAATTA+AGG | - | chr4.2:1567163-1567182 | None:intergenic | 15.0% |
| !! | TAATTAATATTCCAATTAAG+GGG | - | chr4.2:1567161-1567180 | None:intergenic | 15.0% |
| !! | AAAATGAAGTGAATAGTATT+TGG | - | chr4.2:1566683-1566702 | None:intergenic | 20.0% |
| !! | AATTAATATTCCAATTAAGG+GGG | - | chr4.2:1567160-1567179 | None:intergenic | 20.0% |
| !! | AATTTCATTAGCAAAAGATA+TGG | + | chr4.2:1567417-1567436 | MS.gene62693:CDS | 20.0% |
| !! | ATGTCTTCTTTATATCTATA+AGG | - | chr4.2:1567004-1567023 | None:intergenic | 20.0% |
| !! | TGAAATGAATTTGAAAAATG+TGG | - | chr4.2:1567107-1567126 | None:intergenic | 20.0% |
| !! | TTGAGATCAATCAAATATAT+AGG | - | chr4.2:1567190-1567209 | None:intergenic | 20.0% |
| ! | AAAATGTTAGGAGAAGAATA+TGG | + | chr4.2:1567505-1567524 | MS.gene62693:CDS | 25.0% |
| ! | AAATGTTAGGAGAAGAATAT+GGG | + | chr4.2:1567506-1567525 | MS.gene62693:CDS | 25.0% |
| ! | AGATATAAAGAAGACATAGA+TGG | + | chr4.2:1567007-1567026 | MS.gene62693:CDS | 25.0% |
| ! | ATATTTGATTGATCTCAACA+GGG | + | chr4.2:1567192-1567211 | MS.gene62693:intron | 25.0% |
| ! | TATATTTGATTGATCTCAAC+AGG | + | chr4.2:1567191-1567210 | MS.gene62693:intron | 25.0% |
| ! | TTTCACGTTGCAAAAATATT+TGG | + | chr4.2:1566701-1566720 | MS.gene62693:intron | 25.0% |
| AAGAAGACATAGATGGTAAT+AGG | + | chr4.2:1567014-1567033 | MS.gene62693:CDS | 30.0% | |
| AATATTTGGTAGGTGACATA+TGG | + | chr4.2:1566715-1566734 | MS.gene62693:intron | 30.0% | |
| ACGTTGCAAAAATATTTGGT+AGG | + | chr4.2:1566705-1566724 | MS.gene62693:intron | 30.0% | |
| AGATATGGCATATACTATGA+AGG | + | chr4.2:1567432-1567451 | MS.gene62693:CDS | 30.0% | |
| TTGAAAAATGTGGATACCTT+GGG | - | chr4.2:1567097-1567116 | None:intergenic | 30.0% | |
| TTGCAGAGATATAACAACAT+GGG | + | chr4.2:1566608-1566627 | MS.gene62693:CDS | 30.0% | |
| TTTGAAAAATGTGGATACCT+TGG | - | chr4.2:1567098-1567117 | None:intergenic | 30.0% | |
| !! | GAAGGAAGCATTAGATATAT+TGG | + | chr4.2:1567690-1567709 | MS.gene62693:CDS | 30.0% |
| !! | GGATTAGTTGAGGTATTAAT+TGG | + | chr4.2:1567285-1567304 | MS.gene62693:intron | 30.0% |
| ATTCATATGACAGTGCGAAT+TGG | + | chr4.2:1567386-1567405 | MS.gene62693:intron | 35.0% | |
| GTTGCAGAGATATAACAACA+TGG | + | chr4.2:1566607-1566626 | MS.gene62693:CDS | 35.0% | |
| TTCATATGACAGTGCGAATT+GGG | + | chr4.2:1567387-1567406 | MS.gene62693:intron | 35.0% | |
| ! | CTTCTCCTAACATTTTTCTC+AGG | - | chr4.2:1567501-1567520 | None:intergenic | 35.0% |
| !! | ACTTGCTCATTAGTTGCTTT+TGG | + | chr4.2:1567132-1567151 | MS.gene62693:intron | 35.0% |
| AGTGGCCTGAGAAAAATGTT+AGG | + | chr4.2:1567493-1567512 | MS.gene62693:CDS | 40.0% | |
| AGTTGCTCCTTCACAAACAA+AGG | - | chr4.2:1567468-1567487 | None:intergenic | 40.0% | |
| CAAGAGAAGTTTGTCAGCAT+TGG | + | chr4.2:1567662-1567681 | MS.gene62693:CDS | 40.0% | |
| CAGAGATATAACAACATGGG+TGG | + | chr4.2:1566611-1566630 | MS.gene62693:CDS | 40.0% | |
| GTTGCTCCTTCACAAACAAA+GGG | - | chr4.2:1567467-1567486 | None:intergenic | 40.0% | |
| TCAGAGACTAATGTTTCCCA+AGG | + | chr4.2:1567078-1567097 | MS.gene62693:CDS | 40.0% | |
| TCATATGACAGTGCGAATTG+GGG | + | chr4.2:1567388-1567407 | MS.gene62693:intron | 40.0% | |
| TGGTAATAGGAGCAACGAAT+TGG | + | chr4.2:1567027-1567046 | MS.gene62693:CDS | 40.0% | |
| TTTGATTGATCTCAACAGGG+AGG | + | chr4.2:1567195-1567214 | MS.gene62693:intron | 40.0% | |
| ! | CATGTTCCCTTTGTTTGTGA+AGG | + | chr4.2:1567458-1567477 | MS.gene62693:CDS | 40.0% |
| !! | CAACGAATTGGAAGTACTAG+TGG | + | chr4.2:1567039-1567058 | MS.gene62693:CDS | 40.0% |
| AGAGAATCCCTTGAAGAAGC+AGG | + | chr4.2:1567255-1567274 | MS.gene62693:CDS | 45.0% | |
| ATTGATCTCAACAGGGAGGA+TGG | + | chr4.2:1567199-1567218 | MS.gene62693:intron | 45.0% | |
| GTACTACTCACCCTACAACT+TGG | - | chr4.2:1566640-1566659 | None:intergenic | 45.0% | |
| GTTTGTTTGGTATCTCGCTC+TGG | + | chr4.2:1566578-1566597 | MS.gene62693:CDS | 45.0% | |
| TGAAGGAGCAACTTGATCAG+TGG | + | chr4.2:1567475-1567494 | MS.gene62693:CDS | 45.0% | |
| TGTGACTCCTGCTTCTTCAA+GGG | - | chr4.2:1567265-1567284 | None:intergenic | 45.0% | |
| TTGATCTCAACAGGGAGGAT+GGG | + | chr4.2:1567200-1567219 | MS.gene62693:intron | 45.0% | |
| ! | GAGAAGTTTGTCAGCATTGG+TGG | + | chr4.2:1567665-1567684 | MS.gene62693:CDS | 45.0% |
| !! | AGGAGTCACAGGATTAGTTG+AGG | + | chr4.2:1567275-1567294 | MS.gene62693:CDS | 45.0% |
| !! | TTGTCAGCATTGGTGGATGA+AGG | + | chr4.2:1567672-1567691 | MS.gene62693:CDS | 45.0% |
| !! | TTTGGTATCTCGCTCTGGAA+GGG | + | chr4.2:1566583-1566602 | MS.gene62693:CDS | 45.0% |
| !!! | GCTTTTGGAACCCCCTTAAT+TGG | + | chr4.2:1567147-1567166 | MS.gene62693:intron | 45.0% |
| CTACTCACCCTACAACTTGG+CGG | - | chr4.2:1566637-1566656 | None:intergenic | 50.0% | |
| CTGTGACTCCTGCTTCTTCA+AGG | - | chr4.2:1567266-1567285 | None:intergenic | 50.0% | |
| CTTGAAGAAGCAGGAGTCAC+AGG | + | chr4.2:1567264-1567283 | MS.gene62693:CDS | 50.0% | |
| ! | GTTTGGTATCTCGCTCTGGA+AGG | + | chr4.2:1566582-1566601 | MS.gene62693:CDS | 50.0% |
| ATGGGTGGCCGCCAAGTTGT+AGG | + | chr4.2:1566626-1566645 | MS.gene62693:CDS | 60.0% | |
| GACAGTGCGAATTGGGGCAG+TGG | + | chr4.2:1567394-1567413 | MS.gene62693:intron | 60.0% | |
| TGGGTGGCCGCCAAGTTGTA+GGG | + | chr4.2:1566627-1566646 | MS.gene62693:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 1566575 | 1567747 | 1566575 | ID=MS.gene62693 |
| chr4.2 | mRNA | 1566575 | 1567747 | 1566575 | ID=MS.gene62693.t1;Parent=MS.gene62693 |
| chr4.2 | exon | 1566575 | 1566648 | 1566575 | ID=MS.gene62693.t1.exon1;Parent=MS.gene62693.t1 |
| chr4.2 | CDS | 1566575 | 1566648 | 1566575 | ID=cds.MS.gene62693.t1;Parent=MS.gene62693.t1 |
| chr4.2 | exon | 1566994 | 1567099 | 1566994 | ID=MS.gene62693.t1.exon2;Parent=MS.gene62693.t1 |
| chr4.2 | CDS | 1566994 | 1567099 | 1566994 | ID=cds.MS.gene62693.t1;Parent=MS.gene62693.t1 |
| chr4.2 | exon | 1567213 | 1567296 | 1567213 | ID=MS.gene62693.t1.exon3;Parent=MS.gene62693.t1 |
| chr4.2 | CDS | 1567213 | 1567296 | 1567213 | ID=cds.MS.gene62693.t1;Parent=MS.gene62693.t1 |
| chr4.2 | exon | 1567399 | 1567527 | 1567399 | ID=MS.gene62693.t1.exon4;Parent=MS.gene62693.t1 |
| chr4.2 | CDS | 1567399 | 1567527 | 1567399 | ID=cds.MS.gene62693.t1;Parent=MS.gene62693.t1 |
| chr4.2 | exon | 1567646 | 1567747 | 1567646 | ID=MS.gene62693.t1.exon5;Parent=MS.gene62693.t1 |
| chr4.2 | CDS | 1567646 | 1567747 | 1567646 | ID=cds.MS.gene62693.t1;Parent=MS.gene62693.t1 |
| Gene Sequence |
| Protein sequence |
