AlfalfaGEDB | MS.gene93902

M. sativa cultivar XinJiangDaYe / MS.gene93902

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008168 GO:0008171 GO:0016740 GO:0016741
PFAM: PF00891.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93902.t1	XP_024636997.1	94	100	6	0	14	113	264	363	1.40E-47	198.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93902.t1	Q84KK4	71.3	108	31	0	6	113	258	365	2.0e-42	172.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93902.t1	A0A072UTU1	87.0	108	14	0	6	113	256	363	1.3e-47	198.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04918	MS.gene93902	0.801385	9.23E-49	-1.69E-46
MS.gene049254	MS.gene93902	0.839406	1.62E-57	-1.69E-46
MS.gene049404	MS.gene93902	0.806171	9.36E-50	-1.69E-46
MS.gene049499	MS.gene93902	0.818433	1.97E-52	-1.69E-46
MS.gene049711	MS.gene93902	0.825618	4.25E-54	-1.69E-46
MS.gene050026	MS.gene93902	0.827067	1.92E-54	-1.69E-46
MS.gene050273	MS.gene93902	0.80272	4.91E-49	-1.69E-46
MS.gene050559	MS.gene93902	0.813488	2.50E-51	-1.69E-46
MS.gene050561	MS.gene93902	0.81402	1.91E-51	-1.69E-46
MS.gene050562	MS.gene93902	0.803453	3.46E-49	-1.69E-46
MS.gene051279	MS.gene93902	0.827643	1.40E-54	-1.69E-46
MS.gene051594	MS.gene93902	0.803684	3.10E-49	-1.69E-46
MS.gene051719	MS.gene93902	0.80244	5.61E-49	-1.69E-46
MS.gene051720	MS.gene93902	0.806902	6.56E-50	-1.69E-46
MS.gene051744	MS.gene93902	0.810215	1.29E-50	-1.69E-46
MS.gene052016	MS.gene93902	0.806343	8.62E-50	-1.69E-46
MS.gene052037	MS.gene93902	0.80875	2.66E-50	-1.69E-46
MS.gene052239	MS.gene93902	-0.830198	3.36E-55	-1.69E-46
MS.gene052447	MS.gene93902	0.847218	1.33E-59	-1.69E-46
MS.gene052463	MS.gene93902	0.82881	7.31E-55	-1.69E-46
MS.gene052519	MS.gene93902	0.830007	3.74E-55	-1.69E-46
MS.gene052582	MS.gene93902	0.814718	1.34E-51	-1.69E-46
MS.gene052584	MS.gene93902	0.810168	1.32E-50	-1.69E-46
MS.gene05277	MS.gene93902	0.818013	2.45E-52	-1.69E-46
MS.gene053082	MS.gene93902	0.831845	1.33E-55	-1.69E-46
MS.gene05329	MS.gene93902	0.81035	1.20E-50	-1.69E-46
MS.gene05350	MS.gene93902	0.802432	5.63E-49	-1.69E-46
MS.gene05415	MS.gene93902	0.837921	3.91E-57	-1.69E-46
MS.gene054222	MS.gene93902	0.812211	4.75E-51	-1.69E-46
MS.gene05423	MS.gene93902	0.850302	1.85E-60	-1.69E-46
MS.gene054291	MS.gene93902	-0.806794	6.92E-50	-1.69E-46
MS.gene054576	MS.gene93902	0.816062	6.71E-52	-1.69E-46
MS.gene05458	MS.gene93902	0.812903	3.35E-51	-1.69E-46
MS.gene054651	MS.gene93902	0.838635	2.56E-57	-1.69E-46
MS.gene054709	MS.gene93902	0.817194	3.74E-52	-1.69E-46
MS.gene054775	MS.gene93902	0.815793	7.71E-52	-1.69E-46
MS.gene055228	MS.gene93902	0.809091	2.25E-50	-1.69E-46
MS.gene05531	MS.gene93902	0.804037	2.62E-49	-1.69E-46
MS.gene055818	MS.gene93902	0.805228	1.48E-49	-1.69E-46
MS.gene056050	MS.gene93902	0.814318	1.64E-51	-1.69E-46
MS.gene056082	MS.gene93902	0.82395	1.05E-53	-1.69E-46
MS.gene056174	MS.gene93902	0.833771	4.40E-56	-1.69E-46
MS.gene056272	MS.gene93902	0.834244	3.35E-56	-1.69E-46
MS.gene056308	MS.gene93902	0.808184	3.51E-50	-1.69E-46
MS.gene056366	MS.gene93902	0.823008	1.75E-53	-1.69E-46
MS.gene056677	MS.gene93902	0.80604	9.98E-50	-1.69E-46
MS.gene056776	MS.gene93902	0.820476	6.72E-53	-1.69E-46
MS.gene056779	MS.gene93902	0.832742	7.94E-56	-1.69E-46
MS.gene05686	MS.gene93902	0.817154	3.82E-52	-1.69E-46
MS.gene056973	MS.gene93902	0.800033	1.74E-48	-1.69E-46
MS.gene057021	MS.gene93902	0.802677	5.01E-49	-1.69E-46
MS.gene057102	MS.gene93902	0.809342	1.98E-50	-1.69E-46
MS.gene057414	MS.gene93902	0.80599	1.02E-49	-1.69E-46
MS.gene057444	MS.gene93902	0.801546	8.56E-49	-1.69E-46
MS.gene057598	MS.gene93902	0.809176	2.15E-50	-1.69E-46
MS.gene058008	MS.gene93902	0.801627	8.24E-49	-1.69E-46
MS.gene058009	MS.gene93902	0.80895	2.41E-50	-1.69E-46
MS.gene058159	MS.gene93902	0.804423	2.18E-49	-1.69E-46
MS.gene058279	MS.gene93902	0.856901	2.35E-62	-1.69E-46
MS.gene05847	MS.gene93902	0.813162	2.94E-51	-1.69E-46
MS.gene058625	MS.gene93902	0.810726	9.99E-51	-1.69E-46
MS.gene059272	MS.gene93902	0.829184	5.93E-55	-1.69E-46
MS.gene059460	MS.gene93902	0.814994	1.16E-51	-1.69E-46
MS.gene059584	MS.gene93902	0.827084	1.90E-54	-1.69E-46
MS.gene059625	MS.gene93902	0.836846	7.36E-57	-1.69E-46
MS.gene05985	MS.gene93902	-0.80904	2.30E-50	-1.69E-46
MS.gene060134	MS.gene93902	0.804055	2.60E-49	-1.69E-46
MS.gene060257	MS.gene93902	0.812662	3.79E-51	-1.69E-46
MS.gene060373	MS.gene93902	0.818597	1.80E-52	-1.69E-46
MS.gene060634	MS.gene93902	-0.830964	2.18E-55	-1.69E-46
MS.gene060935	MS.gene93902	0.800562	1.36E-48	-1.69E-46
MS.gene060937	MS.gene93902	0.805285	1.44E-49	-1.69E-46
MS.gene061100	MS.gene93902	0.822781	1.97E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93902.t1	MTR_4g033200	87.037	108	14	0	6	113	256	363	7.11e-54	172
MS.gene93902.t1	MTR_4g033015	90.291	103	10	0	11	113	261	363	4.53e-53	170
MS.gene93902.t1	MTR_4g033150	86.408	103	14	0	11	113	261	363	6.32e-51	164
MS.gene93902.t1	MTR_4g088200	64.815	108	38	0	6	113	254	361	1.49e-46	153
MS.gene93902.t1	MTR_4g088190	67.308	104	34	0	10	113	261	364	9.87e-45	148
MS.gene93902.t1	MTR_4g033170	72.222	108	30	0	6	113	250	357	1.89e-36	127
MS.gene93902.t1	MTR_5g074600	57.798	109	45	1	6	113	251	359	3.33e-36	126
MS.gene93902.t1	MTR_5g074610	52.778	108	51	0	6	113	219	326	1.34e-34	121
MS.gene93902.t1	MTR_5g074680	56.481	108	46	1	6	112	252	359	2.61e-34	121
MS.gene93902.t1	MTR_4g088345	52.632	114	52	1	2	113	243	356	3.11e-34	121
MS.gene93902.t1	MTR_5g074580	54.128	109	49	1	6	113	252	360	1.17e-33	119
MS.gene93902.t1	MTR_4g088340	55.000	100	45	0	14	113	234	333	5.32e-32	115
MS.gene93902.t1	MTR_5g074540	53.211	109	50	1	6	113	233	341	7.66e-31	112
MS.gene93902.t1	MTR_7g024520	50.893	112	53	1	2	111	237	348	4.36e-30	110
MS.gene93902.t1	MTR_4g079160	50.000	108	54	0	6	113	248	355	7.86e-30	109
MS.gene93902.t1	MTR_1g076940	53.000	100	46	1	14	113	263	361	1.66e-29	108
MS.gene93902.t1	MTR_7g024500	47.321	112	57	1	2	111	237	348	3.58e-28	105
MS.gene93902.t1	MTR_4g033085	71.296	108	31	0	6	113	250	357	6.24e-28	104
MS.gene93902.t1	MTR_7g014570	46.602	103	54	1	11	113	251	352	1.18e-26	101
MS.gene93902.t1	MTR_7g014510	43.434	99	55	1	15	113	260	357	1.42e-24	95.5
MS.gene93902.t1	MTR_0079s0070	39.423	104	59	3	11	113	234	334	1.81e-16	73.6
MS.gene93902.t1	MTR_4g038440	39.423	104	59	3	11	113	268	368	2.23e-16	73.6
MS.gene93902.t1	MTR_0017s0100	40.385	104	58	3	11	113	268	368	5.45e-16	72.4
MS.gene93902.t1	MTR_3g083620	38.235	102	59	3	10	109	258	357	8.69e-15	68.9
MS.gene93902.t1	MTR_8g024160	40.860	93	51	2	9	100	226	315	1.15e-13	65.5
MS.gene93902.t1	MTR_7g118300	42.553	94	48	3	11	103	275	363	1.75e-13	65.5
MS.gene93902.t1	MTR_8g068870	39.175	97	54	3	11	105	263	356	2.56e-13	64.7
MS.gene93902.t1	MTR_8g068750	40.206	97	53	3	11	105	277	370	2.57e-12	62.0
MS.gene93902.t1	MTR_8g068690	42.353	85	45	2	11	94	276	357	2.67e-12	62.0
MS.gene93902.t1	MTR_8g068760	40.426	94	51	3	14	105	136	226	3.34e-12	60.8
MS.gene93902.t1	MTR_8g068730	39.175	97	54	3	11	105	245	338	3.36e-12	61.6
MS.gene93902.t1	MTR_7g118320	39.326	89	50	2	11	98	262	347	3.62e-12	61.6
MS.gene93902.t1	MTR_1g036490	31.000	100	64	2	15	112	273	369	6.66e-11	58.2
MS.gene93902.t1	MTR_5g098170	35.417	96	57	3	11	105	263	354	7.10e-11	57.8
MS.gene93902.t1	MTR_1g096310	33.721	86	54	1	15	100	276	358	8.60e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93902.t1	AT1G51990	40.678	118	59	6	2	113	250	362	1.77e-15	70.9
MS.gene93902.t1	AT1G51990	40.678	118	59	6	2	113	250	362	1.88e-15	70.9
MS.gene93902.t1	AT4G35160	37.273	110	59	4	11	112	273	380	7.03e-14	66.6
MS.gene93902.t1	AT4G35150	33.636	110	63	3	11	112	216	323	6.44e-13	63.5
MS.gene93902.t1	AT1G33030	32.710	107	65	4	1	103	239	342	1.70e-11	59.7
MS.gene93902.t1	AT1G76790	36.111	108	57	5	1	105	253	351	3.28e-11	58.9
MS.gene93902.t1	AT1G77530	38.462	104	59	3	11	112	280	380	4.50e-11	58.5
MS.gene93902.t1	AT1G77520	34.615	104	63	3	11	112	280	380	9.08e-11	57.8

Find 18 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAGTTCTCCAATCTCTTTA+AGG	0.189882	4.4:+70287134	None:intergenic
ATGATGATGACTTTGTTAAA+TGG	0.247172	4.4:-70286997	MS.gene93902:CDS
TAAAACAAATGAGTTGAAAT+TGG	0.270517	4.4:-70287031	MS.gene93902:CDS
TACAGTGGATTCTGCATGAT+TGG	0.329305	4.4:-70287170	MS.gene93902:intron
TGAATTGTCCTTAAAGAGAT+TGG	0.368275	4.4:-70287142	MS.gene93902:CDS
AAGAAAGAGAGAAGAAAGAA+TGG	0.382447	4.4:-70286972	MS.gene93902:CDS
GATAGACTTAAAGCCGCATA+TGG	0.411079	4.4:+70286903	None:intergenic
AGAAAGAGAGAAGAAAGAAT+GGG	0.461858	4.4:-70286971	MS.gene93902:CDS
ATTTCAGGAAAAGGGACAAA+AGG	0.472680	4.4:-70287102	MS.gene93902:CDS
GAGTGTTATAATAATGATGG+TGG	0.516177	4.4:-70287484	None:intergenic
AATCTTATAGCTGCTGAAGC+CGG	0.565225	4.4:+70286930	None:intergenic
GACAAACTCATATTTGACTC+CGG	0.568072	4.4:-70286949	MS.gene93902:CDS
GGAACGAAACATAATTGAGG+TGG	0.613434	4.4:+70287456	None:intergenic
ATAGACTTAAAGCCGCATAT+GGG	0.613616	4.4:+70286904	None:intergenic
AGGAAAAGGGACAAAAGGAA+AGG	0.628316	4.4:-70287097	MS.gene93902:CDS
ACAGGAACGAAACATAATTG+AGG	0.638025	4.4:+70287453	None:intergenic
GAACGAAACATAATTGAGGT+GGG	0.656586	4.4:+70287457	None:intergenic
TATAAGATTACACCCATATG+CGG	0.675606	4.4:-70286916	MS.gene93902:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAAATATGAACTTATGAA+GGG	+	chr4.4:70287076-70287095	None:intergenic	15.0%
!!!	GATAAAATATTTTTTTACAG+TGG	-	chr4.4:70287171-70287190	MS.gene93902:intron	15.0%
!!	ATTACATGTTCTCTATTTAT+AGG	+	chr4.4:70286971-70286990	None:intergenic	20.0%
!!	GTAAAAATATGAACTTATGA+AGG	+	chr4.4:70287077-70287096	None:intergenic	20.0%
!!!	TAAAACAAATGAGTTGAAAT+TGG	-	chr4.4:70287325-70287344	MS.gene93902:intron	20.0%
!	AAAAACATAATTGAGCTTAC+AGG	+	chr4.4:70286924-70286943	None:intergenic	25.0%
!!	AAAAAAGCTATTTCAGGAAA+AGG	-	chr4.4:70287245-70287264	MS.gene93902:intron	25.0%
!!	AAAAAGCTATTTCAGGAAAA+GGG	-	chr4.4:70287246-70287265	MS.gene93902:intron	25.0%
!!	ATGATGATGACTTTGTTAAA+TGG	-	chr4.4:70287359-70287378	MS.gene93902:intron	25.0%
	AAGAAAGAGAGAAGAAAGAA+TGG	-	chr4.4:70287384-70287403	MS.gene93902:intron	30.0%
	AGAAAGAGAGAAGAAAGAAT+GGG	-	chr4.4:70287385-70287404	MS.gene93902:intron	30.0%
	AGAACATGTAATGCAAAGAA+AGG	-	chr4.4:70286979-70286998	MS.gene93902:CDS	30.0%
	TATAAGATTACACCCATATG+CGG	-	chr4.4:70287440-70287459	MS.gene93902:intron	30.0%
	TGAATTGTCCTTAAAGAGAT+TGG	-	chr4.4:70287214-70287233	MS.gene93902:intron	30.0%
	TGTAATGCAAAGAAAGGATA+CGG	-	chr4.4:70286985-70287004	MS.gene93902:CDS	30.0%
!	AACTGCAAAAAAGCTATTTC+AGG	-	chr4.4:70287239-70287258	MS.gene93902:intron	30.0%
	ACAGGAACGAAACATAATTG+AGG	+	chr4.4:70286906-70286925	None:intergenic	35.0%
	ATAGACTTAAAGCCGCATAT+GGG	+	chr4.4:70287455-70287474	None:intergenic	35.0%
	ATTTCAGGAAAAGGGACAAA+AGG	-	chr4.4:70287254-70287273	MS.gene93902:intron	35.0%
	GAACGAAACATAATTGAGGT+GGG	+	chr4.4:70286902-70286921	None:intergenic	35.0%
	GACAAACTCATATTTGACTC+CGG	-	chr4.4:70287407-70287426	MS.gene93902:intron	35.0%
	GGAAGAAAATGAAAAGCTAC+AGG	+	chr4.4:70286950-70286969	None:intergenic	35.0%
	AATCTTATAGCTGCTGAAGC+CGG	+	chr4.4:70287429-70287448	None:intergenic	40.0%
	AGGAAAAGGGACAAAAGGAA+AGG	-	chr4.4:70287259-70287278	MS.gene93902:intron	40.0%
	GATAGACTTAAAGCCGCATA+TGG	+	chr4.4:70287456-70287475	None:intergenic	40.0%
	GCAGTTCTCCAATCTCTTTA+AGG	+	chr4.4:70287225-70287244	None:intergenic	40.0%
	GGAACGAAACATAATTGAGG+TGG	+	chr4.4:70286903-70286922	None:intergenic	40.0%
!	TACAGTGGATTCTGCATGAT+TGG	-	chr4.4:70287186-70287205	MS.gene93902:intron	40.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	70286885	70287493	70286885	ID=MS.gene93902
chr4.4	mRNA	70286885	70287493	70286885	ID=MS.gene93902.t1;Parent=MS.gene93902
chr4.4	exon	70287455	70287493	70287455	ID=MS.gene93902.t1.exon1;Parent=MS.gene93902.t1
chr4.4	CDS	70287455	70287493	70287455	ID=cds.MS.gene93902.t1;Parent=MS.gene93902.t1
chr4.4	exon	70286885	70287187	70286885	ID=MS.gene93902.t1.exon2;Parent=MS.gene93902.t1
chr4.4	CDS	70286885	70287187	70286885	ID=cds.MS.gene93902.t1;Parent=MS.gene93902.t1

Gene Sequence

>MS.gene93902

ATGATGGTGGATGACCCACCTCAATTATGTTTCGTTCCTGTAAGCTCAATTATGTTTTTGTTCCTGTAGCTTTTCATTTTCTTCCTATAAATAGAGAACATGTAATGCAAAGAAAGGATACGGAATCAACACTTCTCACAATAATTACTTTTTTCTCTTTAGCAACTTTCTCTTCAATTCTCTCTTTTCCCTTCATAAGTTCATATTTTTACAACAGAACTAATATACTCTTAAACAACATATTTTAGCAAAGCTTGATATTTTGTATAGATTTATTAACTAAATAGATAAAATATTTTTTTACAGTGGATTCTGCATGATTGGAATGATGAATTGTCCTTAAAGAGATTGGAGAACTGCAAAAAAGCTATTTCAGGAAAAGGGACAAAAGGAAAGGTGATAATCATTGACATATCAATTGATGAAATAAGTGACAATCATAAAACAAATGAGTTGAAATTGGACTTTGACTTCATGATGATGACTTTGTTAAATGGAAAAGAAAGAGAGAAGAAAGAATGGGACAAACTCATATTTGACTCCGGCTTCAGCAGCTATAAGATTACACCCATATGCGGCTTTAAGTCTATCATCGAAGTTTATCCTTAA

Protein sequence

>MS.gene93902.t1

MMVDDPPQLCFVPWILHDWNDELSLKRLENCKKAISGKGTKGKVIIIDISIDEISDNHKTNELKLDFDFMMMTLLNGKEREKKEWDKLIFDSGFSSYKITPICGFKSIIEVYP