AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068879

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068879.t1 MTR_7g115550 94.583 720 38 1 4 723 8 726 0.0 1317
MS.gene068879.t1 MTR_7g115550 94.606 723 38 1 4 726 8 729 0.0 1315
MS.gene068879.t1 MTR_5g010470 87.396 722 45 4 4 725 8 683 0.0 1174
MS.gene068879.t1 MTR_7g061330 38.095 105 64 1 4 107 62 166 5.49e-15 79.0
MS.gene068879.t1 MTR_7g061330 31.008 129 78 2 4 132 114 231 5.41e-11 66.2
MS.gene068879.t1 MTR_7g461410 38.095 105 64 1 4 107 62 166 5.49e-15 79.0
MS.gene068879.t1 MTR_7g461410 31.008 129 78 2 4 132 114 231 5.41e-11 66.2
MS.gene068879.t1 MTR_7g061330 38.095 105 64 1 4 107 26 130 7.17e-15 78.6
MS.gene068879.t1 MTR_7g061330 31.008 129 78 2 4 132 78 195 7.14e-11 65.9
MS.gene068879.t1 MTR_8g468380 34.021 97 64 0 3 99 22 118 6.32e-13 72.0
MS.gene068879.t1 MTR_3g110028 34.286 105 68 1 4 107 34 138 1.02e-12 72.4
MS.gene068879.t1 MTR_3g110028 36.471 85 54 0 4 88 86 170 3.58e-12 70.5
MS.gene068879.t1 MTR_5g038910 33.945 109 68 1 3 111 26 130 1.42e-12 70.9
MS.gene068879.t1 MTR_1g026870 34.343 99 64 1 4 101 34 132 1.55e-12 71.6
MS.gene068879.t1 MTR_5g038910 33.945 109 68 1 3 111 26 130 2.30e-12 69.7
MS.gene068879.t1 MTR_7g451170 37.647 85 53 0 4 88 192 276 4.83e-12 68.9
MS.gene068879.t1 MTR_4g088015 35.294 85 55 0 4 88 222 306 1.01e-11 68.2
MS.gene068879.t1 MTR_7g035350 33.981 103 65 1 5 104 227 329 1.15e-11 68.2
MS.gene068879.t1 MTR_7g037260 36.471 85 54 0 4 88 146 230 2.51e-11 66.2
MS.gene068879.t1 MTR_4g105660 37.647 85 53 0 4 88 181 265 3.93e-11 66.2
MS.gene068879.t1 MTR_6g027340 34.783 92 60 0 6 97 19 110 4.07e-11 63.5
MS.gene068879.t1 MTR_6g027360 35.052 97 63 0 1 97 76 172 6.26e-11 64.7
MS.gene068879.t1 MTR_5g010650 37.349 83 52 0 6 88 113 195 6.58e-11 65.9
MS.gene068879.t1 MTR_6g074860 35.632 87 56 0 2 88 3 89 7.33e-11 63.5
MS.gene068879.t1 MTR_1g057980 32.258 93 63 0 4 96 81 173 7.44e-11 64.3
MS.gene068879.t1 MTR_6g027370 31.959 97 66 0 4 100 100 196 8.33e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068879.t1 AT1G09770 75.485 722 171 4 4 722 8 726 0.0 1050
MS.gene068879.t1 AT5G02320 37.143 105 65 1 4 107 76 180 1.73e-14 77.4
MS.gene068879.t1 AT5G02320 34.118 85 56 0 4 88 128 212 9.03e-11 65.5
MS.gene068879.t1 AT5G02320 37.143 105 65 1 4 107 76 180 1.73e-14 77.4
MS.gene068879.t1 AT5G02320 34.118 85 56 0 4 88 128 212 9.03e-11 65.5
MS.gene068879.t1 AT5G02320 37.143 105 65 1 4 107 16 120 1.97e-14 77.0
MS.gene068879.t1 AT3G18100 34.375 96 63 0 4 99 547 642 6.27e-14 76.3
MS.gene068879.t1 AT3G18100 36.082 97 60 2 6 101 498 593 2.40e-11 67.8
MS.gene068879.t1 AT3G18100 34.375 96 63 0 4 99 499 594 1.05e-13 75.5
MS.gene068879.t1 AT3G18100 36.082 97 60 2 6 101 450 545 3.94e-11 67.0
MS.gene068879.t1 AT3G09370 36.190 105 66 1 4 107 84 188 1.25e-13 74.7
MS.gene068879.t1 AT3G18100 34.375 96 63 0 4 99 386 481 1.28e-13 75.1
MS.gene068879.t1 AT3G18100 36.082 97 60 2 6 101 337 432 5.05e-11 66.6
MS.gene068879.t1 AT3G09370 36.190 105 66 1 4 107 79 183 1.32e-13 74.3
MS.gene068879.t1 AT5G11510 37.374 99 61 1 4 101 30 128 1.35e-13 75.1
MS.gene068879.t1 AT5G11510 37.374 99 61 1 4 101 30 128 1.35e-13 75.1
MS.gene068879.t1 AT5G11510 37.374 99 61 1 4 101 30 128 1.35e-13 75.1
MS.gene068879.t1 AT3G18100 34.375 96 63 0 4 99 334 429 1.63e-13 74.7
MS.gene068879.t1 AT3G18100 36.082 97 60 2 6 101 285 380 6.64e-11 66.2
MS.gene068879.t1 AT3G09370 36.190 105 66 1 4 107 92 196 1.66e-13 74.3
MS.gene068879.t1 AT5G11510 37.374 99 61 1 4 101 30 128 2.20e-13 74.3
MS.gene068879.t1 AT5G11510 37.374 99 61 1 4 101 30 128 2.74e-13 73.9
MS.gene068879.t1 AT4G18770 38.824 85 52 0 4 88 218 302 9.14e-13 71.2
MS.gene068879.t1 AT4G32730 35.354 99 63 1 4 101 36 134 1.42e-12 72.0
MS.gene068879.t1 AT4G32730 35.354 99 63 1 4 101 36 134 1.42e-12 72.0
MS.gene068879.t1 AT4G32730 35.354 99 63 1 4 101 36 134 1.42e-12 72.0
MS.gene068879.t1 AT4G32730 35.354 99 63 1 4 101 36 134 1.42e-12 72.0
MS.gene068879.t1 AT4G32730 35.354 99 63 1 4 101 36 134 2.42e-12 70.9
MS.gene068879.t1 AT4G33450 32.990 97 65 0 4 100 20 116 5.35e-12 67.0
MS.gene068879.t1 AT2G02820 30.579 121 70 1 3 109 30 150 8.43e-12 68.6
MS.gene068879.t1 AT2G02820 30.579 121 70 1 3 109 30 150 1.18e-11 68.2
MS.gene068879.t1 AT4G00540 37.500 112 61 2 4 106 47 158 1.35e-11 67.8
MS.gene068879.t1 AT4G00540 37.500 112 61 2 4 106 45 156 1.78e-11 67.4
MS.gene068879.t1 AT1G14350 31.959 97 66 0 3 99 25 121 2.39e-11 67.0
MS.gene068879.t1 AT1G14350 31.959 97 66 0 3 99 25 121 2.39e-11 67.0
MS.gene068879.t1 AT2G02820 30.579 121 70 1 3 109 30 150 2.55e-11 66.2
MS.gene068879.t1 AT5G40360 35.294 85 55 0 4 88 159 243 2.77e-11 66.2
MS.gene068879.t1 AT5G17800 34.343 99 65 0 2 100 92 190 3.84e-11 65.5
MS.gene068879.t1 AT1G17950 31.313 99 68 0 2 100 4 102 4.59e-11 64.3
MS.gene068879.t1 AT4G38620 31.655 139 84 5 4 138 15 146 5.73e-11 64.7

Find 209 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTCTTTCCAACTGTTTC+TGG 0.013419 2.1:-46652209 None:intergenic
TTCTGGGTCATTCAGCTTCT+TGG 0.153986 2.1:-46652192 None:intergenic
TGCCAGCGCCATACCACTTC+TGG 0.197747 2.1:-46652624 None:intergenic
ATTTCATAACAGCAGCTTTC+AGG 0.225007 2.1:-46651401 None:intergenic
CAGCATCTCTGGAGCTTATT+AGG 0.228626 2.1:+46652993 MS.gene068879:CDS
GGAGTAAGGCCTGCACTTCC+AGG 0.240853 2.1:-46652602 None:intergenic
TTGGGTGTCTGTGGATAATT+AGG 0.241982 2.1:-46652362 None:intergenic
CATGATGGAAGCTGAAAATT+TGG 0.248037 2.1:+46652445 MS.gene068879:CDS
AAAGGAGCTTCCAGCCTTAC+TGG 0.273790 2.1:+46653096 MS.gene068879:CDS
GTTCTCTGAGGAGTTCTTAA+AGG 0.283026 2.1:-46652398 None:intergenic
AAAGCTGCTGTTATGAAATA+TGG 0.287568 2.1:+46651405 MS.gene068879:CDS
GAATTGGTTGCATCACTATC+TGG 0.302904 2.1:-46652823 None:intergenic
GGTCGCGCAGGCTTCGATTC+AGG 0.310060 2.1:-46651744 None:intergenic
AGTCCACTCAGTCTTCTTAA+TGG 0.314486 2.1:-46651517 None:intergenic
GTTTGAGAAAAGGCCTCCTT+TGG 0.314999 2.1:+46651995 MS.gene068879:CDS
CCCTGTCATAGAAGATTATC+AGG 0.315605 2.1:+46653207 MS.gene068879:CDS
CCATTGCCTCACGAAGATAC+TGG 0.317289 2.1:-46653937 None:intergenic
TGGGGTCACTCCTAAGAAAA+AGG 0.319124 2.1:+46652538 MS.gene068879:CDS
ATCTCTTTCCAACTGTTTCT+GGG 0.330928 2.1:-46652208 None:intergenic
TCTTCAGCATCTTCTTGAAT+TGG 0.331097 2.1:-46652839 None:intergenic
ATCTCGTCGGCTTGCTCAAT+AGG 0.342238 2.1:-46653067 None:intergenic
TCAAGTTCTTCTATAGTAGT+AGG 0.344179 2.1:-46652068 None:intergenic
TAGGCCTCCTCCAGCATCTC+TGG 0.367513 2.1:+46652982 MS.gene068879:CDS
CAGAAATTCCGTTTGAGAAA+AGG 0.368252 2.1:+46651985 MS.gene068879:CDS
GCTTGAGGAGATAGCAAATA+AGG 0.373696 2.1:+46653141 MS.gene068879:CDS
CCTTGACGCCACTTGTGTTA+AGG 0.386161 2.1:+46651659 MS.gene068879:CDS
TCTCGTCGGCTTGCTCAATA+GGG 0.387795 2.1:-46653066 None:intergenic
AGGCCCATTTGCTGCTCGTT+TGG 0.388358 2.1:-46653179 None:intergenic
TCAGATTTCCGATCAAGAAT+TGG 0.389053 2.1:+46652253 MS.gene068879:CDS
GCAGGCTTCGATTCAGGATT+CGG 0.393602 2.1:-46651738 None:intergenic
CCAAAATGAGCATAGGTGTT+TGG 0.397192 2.1:+46651358 None:intergenic
CTACGAAGGGAACTCCTCTC+AGG 0.399722 2.1:+46652672 MS.gene068879:CDS
AGGCTGCTGGGATTGATATA+AGG 0.409355 2.1:+46651925 MS.gene068879:CDS
TCTGCAACCCCTGGAAGTGC+AGG 0.411721 2.1:+46652593 MS.gene068879:CDS
AGAGATGCTGGAGGAGGCCT+AGG 0.415805 2.1:-46652980 None:intergenic
CATGGTCTATCAAGTGTTGC+TGG 0.417267 2.1:+46654046 MS.gene068879:CDS
GGATCCAAACGAGCAGCAAA+TGG 0.417377 2.1:+46653175 MS.gene068879:CDS
TTGATAGACCATGTGTGTTA+CGG 0.418408 2.1:-46654036 None:intergenic
GCTACATATCAATGAAGATA+TGG 0.420115 2.1:+46652700 MS.gene068879:CDS
TAAGGCAGCGGAGGAGGAAA+AGG 0.424744 2.1:+46651943 MS.gene068879:CDS
TGATAGACCATGTGTGTTAC+GGG 0.426495 2.1:-46654035 None:intergenic
TTGGATGAAATTGCCAAGTT+GGG 0.429250 2.1:+46652272 MS.gene068879:CDS
AGCTTTCAGGATTTCATCCT+CGG 0.436489 2.1:-46651388 None:intergenic
CAGTATCTTCGTGAGGCAAT+GGG 0.437430 2.1:+46653938 MS.gene068879:CDS
ATTGGATGAAATTGCCAAGT+TGG 0.444138 2.1:+46652271 MS.gene068879:CDS
AAGAACTTGAGGGGAAAAGA+AGG 0.444403 2.1:+46652081 MS.gene068879:CDS
GCCAGCGCCATACCACTTCT+GGG 0.445237 2.1:-46652623 None:intergenic
GCAAATAAGGAGAGAGAGAA+AGG 0.446065 2.1:+46653154 MS.gene068879:CDS
CTAATAAGCTCCAGAGATGC+TGG 0.447893 2.1:-46652992 None:intergenic
ATCCTCTTCAATCTTCTCCT+CGG 0.449369 2.1:-46652864 None:intergenic
GAGGGAGTCAGCAGTGGGAT+TGG 0.449422 2.1:-46652572 None:intergenic
CCATACCACTTCTGGGAGTA+AGG 0.450702 2.1:-46652616 None:intergenic
AAGAGAGAATTGAAGGCTGC+TGG 0.451049 2.1:+46651912 MS.gene068879:CDS
TCCTGATAATCTTCTATGAC+AGG 0.451422 2.1:-46653208 None:intergenic
TCATACTCATCCATATCAAC+AGG 0.454291 2.1:-46651771 None:intergenic
ACCCACAATGGTATCACTAA+TGG 0.455710 2.1:-46651598 None:intergenic
AGAAGAAGAGGCACGGCAGA+AGG 0.457675 2.1:+46652916 MS.gene068879:CDS
TCCCCTGGACGCAATTTCCT+AGG 0.458724 2.1:-46651708 None:intergenic
AAAGGAGTCAAGGCTTGGTT+GGG 0.461730 2.1:-46652380 None:intergenic
TGCAGAGAGGCAAGATGCCC+CGG 0.470266 2.1:+46652154 MS.gene068879:CDS
GAGTCTCAGACACCACTGTT+AGG 0.471291 2.1:+46652479 MS.gene068879:CDS
CTGAAAATTTGGCCAGGCTG+AGG 0.471566 2.1:+46652456 MS.gene068879:CDS
TTCTTAAAGGAGTCAAGGCT+TGG 0.471836 2.1:-46652385 None:intergenic
AATCTGAAGGATGCAACTCC+AGG 0.473048 2.1:-46652511 None:intergenic
TGGCATGCAGAATAGCAGCC+GGG 0.473211 2.1:-46652172 None:intergenic
TTGATATAAGGCAGCGGAGG+AGG 0.474103 2.1:+46651937 MS.gene068879:CDS
AGCCAACCGAGCACATGCTT+CGG 0.476372 2.1:-46651808 None:intergenic
TGATGTTGCTGATGAGGATA+GGG 0.479449 2.1:+46652025 MS.gene068879:CDS
AATTTCATCCAATTCTTGAT+CGG 0.479560 2.1:-46652261 None:intergenic
GCGACCCGATCCTGTTGATA+TGG 0.481942 2.1:+46651761 MS.gene068879:CDS
CCAGTATCTTCGTGAGGCAA+TGG 0.484875 2.1:+46653937 MS.gene068879:CDS
TGTCTGAGACTCCCTCAGCC+TGG 0.484905 2.1:-46652468 None:intergenic
AACGATCAAAAGTCATTCAA+AGG 0.485643 2.1:+46652951 MS.gene068879:CDS
TCCTCTTCAATCTTCTCCTC+GGG 0.488427 2.1:-46652863 None:intergenic
GAGTAAGGCCTGCACTTCCA+GGG 0.490905 2.1:-46652601 None:intergenic
CTCCTTTCTTATCATCTCGT+CGG 0.493278 2.1:-46653080 None:intergenic
TCATTCAAAGGGAACTTCCT+AGG 0.493709 2.1:+46652963 MS.gene068879:CDS
TGCACTTCCAGGGGTTGCAG+AGG 0.493979 2.1:-46652591 None:intergenic
ACGAGTAGCACTGCTGCCTT+CGG 0.495690 2.1:-46652330 None:intergenic
CTGACTCCCTCTGCAACCCC+TGG 0.496434 2.1:+46652584 MS.gene068879:CDS
TTCCGAAGCATGTGCTCGGT+TGG 0.496711 2.1:+46651806 MS.gene068879:CDS
TCTTCTATGACAGGGATAGC+AGG 0.496725 2.1:-46653199 None:intergenic
GTCTCCGTCAGCTCTTATCA+AGG 0.499862 2.1:-46653020 None:intergenic
TTGTCATGCTCCAGTAAGGC+TGG 0.499885 2.1:-46653106 None:intergenic
TGCTTTCCGAAGCATGTGCT+CGG 0.502006 2.1:+46651802 MS.gene068879:CDS
GTGAGAAGTAAGCTGGATGA+TGG 0.502979 2.1:+46654112 MS.gene068879:CDS
CTCATCCATATCAACAGGAT+CGG 0.503902 2.1:-46651766 None:intergenic
AAGTAAGCTGGATGATGGGA+AGG 0.506469 2.1:+46654117 MS.gene068879:CDS
ATAGCATCTCCTTTACTAGC+TGG 0.506651 2.1:-46652422 None:intergenic
AGAGGATATGTCTGATAGAA+TGG 0.507303 2.1:+46652880 MS.gene068879:CDS
AGCTCCAGAGATGCTGGAGG+AGG 0.508047 2.1:-46652986 None:intergenic
GATGATTCGACTTGAGAAGA+AGG 0.511403 2.1:+46654144 MS.gene068879:CDS
TGATAGAATGGCTAGAGAAA+AGG 0.511992 2.1:+46652892 MS.gene068879:CDS
ATTGCTGCCCACAAGGTCAC+TGG 0.512710 2.1:-46652300 None:intergenic
TTGGCATGCAGAATAGCAGC+CGG 0.514383 2.1:-46652173 None:intergenic
TTTGAGAAAAGGCCTCCTTT+GGG 0.515050 2.1:+46651996 MS.gene068879:CDS
CAGAGAACACCAGCTAGTAA+AGG 0.515217 2.1:+46652413 MS.gene068879:CDS
GTTGCAGAGGGAGTCAGCAG+TGG 0.516056 2.1:-46652578 None:intergenic
TGCAGATTTGCAAACGAGTA+GGG 0.518174 2.1:-46651454 None:intergenic
CATTGCCTCACGAAGATACT+GGG 0.519621 2.1:-46653936 None:intergenic
TGGGTGTCTGTGGATAATTA+GGG 0.519886 2.1:-46652361 None:intergenic
TTGATGTTGCTGATGAGGAT+AGG 0.524642 2.1:+46652024 MS.gene068879:CDS
TCGGCTTGCTCAATAGGGGT+TGG 0.524733 2.1:-46653061 None:intergenic
TGAAAATTTGGCCAGGCTGA+GGG 0.525084 2.1:+46652457 MS.gene068879:CDS
GACCCTAGGAAATTGCGTCC+AGG 0.525314 2.1:+46651705 MS.gene068879:CDS
CTGCAGATTTGCAAACGAGT+AGG 0.526600 2.1:-46651455 None:intergenic
AGCATTGTCATGCTCCAGTA+AGG 0.527412 2.1:-46653110 None:intergenic
ACCATTAGTGATACCATTGT+GGG 0.527710 2.1:+46651597 MS.gene068879:CDS
CAAGTTCTTCTATAGTAGTA+GGG 0.528361 2.1:-46652067 None:intergenic
TAAAGGAGTCAAGGCTTGGT+TGG 0.528607 2.1:-46652381 None:intergenic
GAAGTGGTATGGCGCTGGCA+AGG 0.528663 2.1:+46652627 MS.gene068879:CDS
TGAGAATGTGAGAAGTAAGC+TGG 0.529343 2.1:+46654105 MS.gene068879:CDS
GCTTGCTCAATAGGGGTTGG+TGG 0.531592 2.1:-46653058 None:intergenic
CACTCAGTCTTCTTAATGGA+AGG 0.532258 2.1:-46651513 None:intergenic
GCACTTCCAGGGGTTGCAGA+GGG 0.532965 2.1:-46652590 None:intergenic
TCATCCATATCAACAGGATC+GGG 0.534614 2.1:-46651765 None:intergenic
ACCCTAGGAAATTGCGTCCA+GGG 0.536757 2.1:+46651706 MS.gene068879:CDS
AAGTGGTATGGCGCTGGCAA+GGG 0.537683 2.1:+46652628 MS.gene068879:CDS
TCCCAGAAGTGGTATGGCGC+TGG 0.538783 2.1:+46652622 MS.gene068879:CDS
ACTGTTAGGAGGAGAGAACC+TGG 0.540907 2.1:+46652493 MS.gene068879:CDS
GATCCAAACGAGCAGCAAAT+GGG 0.542547 2.1:+46653176 MS.gene068879:CDS
AGAGAGAATTGAAGGCTGCT+GGG 0.549517 2.1:+46651913 MS.gene068879:CDS
TGATCGGAAATCTGAGGTGG+TGG 0.551298 2.1:-46652245 None:intergenic
CCTTACTCCCAGAAGTGGTA+TGG 0.551739 2.1:+46652616 MS.gene068879:CDS
AGCCGACGAGATGATAAGAA+AGG 0.552168 2.1:+46653078 MS.gene068879:CDS
GAAAAGAAGGATTGATGTTG+AGG 0.552508 2.1:+46652094 MS.gene068879:CDS
GGTTATGCCAGTGACCTTGT+GGG 0.556103 2.1:+46652293 MS.gene068879:CDS
TGAGAAAGCAAGACGTTGCA+AGG 0.556741 2.1:+46652123 MS.gene068879:CDS
GGGTTATGCCAGTGACCTTG+TGG 0.560009 2.1:+46652292 MS.gene068879:CDS
GGATTCGGATCAATCTCCCC+TGG 0.561665 2.1:-46651723 None:intergenic
CCCCTGGACGCAATTTCCTA+GGG 0.562986 2.1:-46651707 None:intergenic
AGAAACAGCTTGAGGAAGCA+AGG 0.565360 2.1:+46651868 MS.gene068879:CDS
TCAACAGGATCGGGTCGCGC+AGG 0.566355 2.1:-46651756 None:intergenic
ACGATCAAAAGTCATTCAAA+GGG 0.567785 2.1:+46652952 MS.gene068879:CDS
AGGAGTTCTTAAAGGAGTCA+AGG 0.570143 2.1:-46652390 None:intergenic
ACTACTATAGAAGAACTTGA+GGG 0.571318 2.1:+46652071 MS.gene068879:CDS
TTGCAGAGGGAGTCAGCAGT+GGG 0.571318 2.1:-46652577 None:intergenic
TAAAGGAGATGCTATCATGA+TGG 0.573963 2.1:+46652430 MS.gene068879:CDS
CTGAATGACCCAGAAACAGT+TGG 0.574442 2.1:+46652200 MS.gene068879:CDS
TTACTAGCTGGTGTTCTCTG+AGG 0.578802 2.1:-46652410 None:intergenic
TAGAGAAAAGGCAGAAGAAG+AGG 0.579560 2.1:+46652904 MS.gene068879:CDS
ATGAACCTGGTGATGACCCT+AGG 0.580871 2.1:+46651691 MS.gene068879:CDS
TTATTTGCTATCTCCTCAAG+CGG 0.583374 2.1:-46653139 None:intergenic
GTCACTGGCATAACCCAACT+TGG 0.585958 2.1:-46652285 None:intergenic
AAGGTTACATTACTCACGCA+AGG 0.586053 2.1:+46654163 MS.gene068879:CDS
AGTAAGGCCTGCACTTCCAG+GGG 0.586407 2.1:-46652600 None:intergenic
GTAAAGCTCGTTGGTATGAG+TGG 0.588526 2.1:+46651484 MS.gene068879:CDS
AACTCCTTGATAAGAGCTGA+CGG 0.589304 2.1:+46653016 MS.gene068879:CDS
AAGGCTTGGTTGGGTGTCTG+TGG 0.589342 2.1:-46652371 None:intergenic
CCTGATAATCTTCTATGACA+GGG 0.589597 2.1:-46653207 None:intergenic
GACTGAGTGGACTCGAGAAG+AGG 0.592125 2.1:+46651527 MS.gene068879:CDS
TGCTTCAACAAAATCCTCGA+GGG 0.592155 2.1:-46653975 None:intergenic
CCTTAACACAAGTGGCGTCA+AGG 0.592424 2.1:-46651659 None:intergenic
GGTATGGCGCTGGCAAGGGA+TGG 0.593619 2.1:+46652632 MS.gene068879:CDS
CTCCTTCGCAGTGTCTTGAA+CGG 0.594673 2.1:+46651625 MS.gene068879:CDS
TGCTGATGAGGATAGGGAGG+TGG 0.595277 2.1:+46652031 MS.gene068879:CDS
CAGAACGGTGTAAAGCTCGT+TGG 0.595953 2.1:+46651475 MS.gene068879:CDS
AGTGTTGCCAAAATGAGCAT+AGG 0.596521 2.1:+46651351 None:intergenic
AACCATTAGTGATACCATTG+TGG 0.597390 2.1:+46651596 MS.gene068879:CDS
TGAGAAGTAAGCTGGATGAT+GGG 0.598085 2.1:+46654113 MS.gene068879:CDS
TACTACTATAGAAGAACTTG+AGG 0.598439 2.1:+46652070 MS.gene068879:CDS
AAAAGGCAGAAGAAGAGGCA+CGG 0.599691 2.1:+46652909 MS.gene068879:CDS
TGAGTTCATTGCTGCCCACA+AGG 0.602663 2.1:-46652307 None:intergenic
CTAATGGTTCTCCACTGAGT+AGG 0.603402 2.1:-46651582 None:intergenic
TGGAAGCTGAAAATTTGGCC+AGG 0.607474 2.1:+46652450 MS.gene068879:CDS
GCTCGGTTGGCTAACACGAA+AGG 0.608258 2.1:+46651819 MS.gene068879:CDS
TCTCAGACACCACTGTTAGG+AGG 0.609284 2.1:+46652482 MS.gene068879:CDS
AGCGGAGGAGGAAAAGGAGA+GGG 0.609580 2.1:+46651949 MS.gene068879:CDS
CAATGCTAAGTATCCGCTTG+AGG 0.613956 2.1:+46653126 MS.gene068879:CDS
TACGAAGGGAACTCCTCTCA+GGG 0.614373 2.1:+46652673 MS.gene068879:CDS
AATTTCCTAGGGTCATCACC+AGG 0.619740 2.1:-46651696 None:intergenic
CAGCGGAGGAGGAAAAGGAG+AGG 0.619802 2.1:+46651948 MS.gene068879:CDS
CTGGGATTGATATAAGGCAG+CGG 0.621106 2.1:+46651931 MS.gene068879:CDS
GTTATCGTCCTTAACACAAG+TGG 0.622208 2.1:-46651667 None:intergenic
GCAGGCCTTACTCCCAGAAG+TGG 0.624004 2.1:+46652611 MS.gene068879:CDS
CGAAGGAGTACGACCCACAA+TGG 0.624367 2.1:-46651610 None:intergenic
CCCTAGGAAATTGCGTCCAG+GGG 0.631019 2.1:+46651707 MS.gene068879:CDS
CTCGTCGGCTTGCTCAATAG+GGG 0.633774 2.1:-46653065 None:intergenic
TTTGATACCCGTAACACACA+TGG 0.638610 2.1:+46654028 MS.gene068879:CDS
GGCATGCAGAATAGCAGCCG+GGG 0.639810 2.1:-46652171 None:intergenic
AAGGACGATAACTATGAACC+TGG 0.641325 2.1:+46651678 MS.gene068879:CDS
TGTTGCTGATGAGGATAGGG+AGG 0.645550 2.1:+46652028 MS.gene068879:CDS
AGGTTCTCTCCTCCTAACAG+TGG 0.645695 2.1:-46652491 None:intergenic
GCAACATCAAAATAACCCAA+AGG 0.645993 2.1:-46652011 None:intergenic
AGAAGATGCTGAAGAGCCCG+AGG 0.646686 2.1:+46652847 MS.gene068879:CDS
CTACTATAGAAGAACTTGAG+GGG 0.654103 2.1:+46652072 MS.gene068879:CDS
GCCCGAGGAGAAGATTGAAG+AGG 0.654306 2.1:+46652862 MS.gene068879:CDS
AATTCTTGATCGGAAATCTG+AGG 0.665249 2.1:-46652251 None:intergenic
AGCTAGGGAGAAACAGCTTG+AGG 0.665565 2.1:+46651860 MS.gene068879:CDS
TACCGTTCAAGACACTGCGA+AGG 0.666095 2.1:-46651627 None:intergenic
AGGTTACATTACTCACGCAA+GGG 0.667391 2.1:+46654164 MS.gene068879:CDS
GGCAGCAATGAACTCACCGA+AGG 0.667861 2.1:+46652314 MS.gene068879:CDS
CTAAGCTTACGCCTACTCAG+TGG 0.671805 2.1:+46651571 MS.gene068879:CDS
TCGTTTGCAAATCTGCAGAA+CGG 0.675444 2.1:+46651460 MS.gene068879:CDS
TCTTGATCGGAAATCTGAGG+TGG 0.678038 2.1:-46652248 None:intergenic
ACATCAAAATAACCCAAAGG+AGG 0.679868 2.1:-46652008 None:intergenic
CTTCCATTAAGAAGACTGAG+TGG 0.680364 2.1:+46651514 MS.gene068879:CDS
TGAAGCCCAGTATCTTCGTG+AGG 0.688109 2.1:+46653931 MS.gene068879:CDS
GTGCTTCAACAAAATCCTCG+AGG 0.688213 2.1:-46653976 None:intergenic
ATATGTAGCTCATCCCTGAG+AGG 0.688581 2.1:-46652686 None:intergenic
AGTATCTTCGTGAGGCAATG+GGG 0.691770 2.1:+46653939 MS.gene068879:CDS
GGATTGATATAAGGCAGCGG+AGG 0.699913 2.1:+46651934 MS.gene068879:CDS
ATAAGCTCCAGAGATGCTGG+AGG 0.701115 2.1:-46652989 None:intergenic
AACAGCTTGAGGAAGCAAGG+AGG 0.704973 2.1:+46651871 MS.gene068879:CDS
TGATATGGATGAGTATGAGA+AGG 0.714958 2.1:+46651776 MS.gene068879:CDS
TGTCATAGAAGATTATCAGG+AGG 0.727087 2.1:+46653210 MS.gene068879:CDS
GCATGAAAATGATTCCCTCG+AGG 0.731393 2.1:+46653961 MS.gene068879:CDS
CAAGGAATAAAATTGCAGAG+AGG 0.745082 2.1:+46652141 MS.gene068879:CDS
AGGTGTTTGGACAAACACCG+AGG 0.789261 2.1:+46651371 MS.gene068879:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGGCATAAAAATAAAATTAA+AGG - chr2.1:46653277-46653296 None:intergenic 15.0%
!! TTATAACTGAAAAAAAAACA+AGG - chr2.1:46653618-46653637 None:intergenic 15.0%
!! AAGTTTGTATTACTATCATT+CGG - chr2.1:46653770-46653789 None:intergenic 20.0%
!! ATAATAATTAACAAGCAGTT+TGG - chr2.1:46653793-46653812 None:intergenic 20.0%
! AATTTCATCCAATTCTTGAT+CGG - chr2.1:46652264-46652283 None:intergenic 25.0%
! GAAATATGGTAAAAATCAGT+GGG + chr2.1:46651419-46651438 MS.gene068879:CDS 25.0%
! GATGCCAAGTAATTAATAAT+CGG - chr2.1:46653438-46653457 None:intergenic 25.0%
! TAGGGAATAAATGTTGTAAA+AGG - chr2.1:46653733-46653752 None:intergenic 25.0%
! TGAAATATGGTAAAAATCAG+TGG + chr2.1:46651418-46651437 MS.gene068879:CDS 25.0%
! TTGTCCGATTATTAATTACT+TGG + chr2.1:46653431-46653450 MS.gene068879:intron 25.0%
!! ATGTGAATTTCCTTTTTCTT+AGG - chr2.1:46652551-46652570 None:intergenic 25.0%
!! ATTTTTACATAAGATTCCAG+TGG - chr2.1:46653500-46653519 None:intergenic 25.0%
!! TTTTATCTGTTGTTCTCAAT+AGG + chr2.1:46653889-46653908 MS.gene068879:intron 25.0%
AAAAAGCAAAAAGGAAAGCT+AGG + chr2.1:46651844-46651863 MS.gene068879:CDS 30.0%
AAAAGCAAAAAGGAAAGCTA+GGG + chr2.1:46651845-46651864 MS.gene068879:CDS 30.0%
AAAGCTGCTGTTATGAAATA+TGG + chr2.1:46651405-46651424 MS.gene068879:CDS 30.0%
AACGATCAAAAGTCATTCAA+AGG + chr2.1:46652951-46652970 MS.gene068879:CDS 30.0%
AATATGCAACTAGCTCATAA+AGG - chr2.1:46653566-46653585 None:intergenic 30.0%
ACAGAAAAAGAGAGAATTGA+AGG + chr2.1:46651905-46651924 MS.gene068879:CDS 30.0%
ACGATCAAAAGTCATTCAAA+GGG + chr2.1:46652952-46652971 MS.gene068879:CDS 30.0%
ACTACTATAGAAGAACTTGA+GGG + chr2.1:46652071-46652090 MS.gene068879:CDS 30.0%
ATGAAGAAAAGCGAAGTAAA+AGG - chr2.1:46653297-46653316 None:intergenic 30.0%
CAAGTTCTTCTATAGTAGTA+GGG - chr2.1:46652070-46652089 None:intergenic 30.0%
CATCTCCATAGTCAAATTTA+AGG - chr2.1:46653822-46653841 None:intergenic 30.0%
CGAAAGGTAAAAAAGCAAAA+AGG + chr2.1:46651835-46651854 MS.gene068879:CDS 30.0%
GCTACATATCAATGAAGATA+TGG + chr2.1:46652700-46652719 MS.gene068879:CDS 30.0%
TACTACTATAGAAGAACTTG+AGG + chr2.1:46652070-46652089 MS.gene068879:CDS 30.0%
TATGAGCTAGTTGCATATTA+TGG + chr2.1:46653567-46653586 MS.gene068879:intron 30.0%
TCAAGTTCTTCTATAGTAGT+AGG - chr2.1:46652071-46652090 None:intergenic 30.0%
TGACTCCTTAAATTTGACTA+TGG + chr2.1:46653814-46653833 MS.gene068879:intron 30.0%
!! ACTATCTGGTACTCATTTTT+AGG - chr2.1:46652812-46652831 None:intergenic 30.0%
!!! TTATTTTGATGTTGCTGATG+AGG + chr2.1:46652019-46652038 MS.gene068879:CDS 30.0%
AACCATTAGTGATACCATTG+TGG + chr2.1:46651596-46651615 MS.gene068879:CDS 35.0%
AAGATTCCAGTGGTTTCAAA+GGG - chr2.1:46653490-46653509 None:intergenic 35.0%
AATTCTTGATCGGAAATCTG+AGG - chr2.1:46652254-46652273 None:intergenic 35.0%
ACATCAAAATAACCCAAAGG+AGG - chr2.1:46652011-46652030 None:intergenic 35.0%
ACCATTAGTGATACCATTGT+GGG + chr2.1:46651597-46651616 MS.gene068879:CDS 35.0%
AGAGGATATGTCTGATAGAA+TGG + chr2.1:46652880-46652899 MS.gene068879:CDS 35.0%
ATTGGATGAAATTGCCAAGT+TGG + chr2.1:46652271-46652290 MS.gene068879:CDS 35.0%
ATTTCATAACAGCAGCTTTC+AGG - chr2.1:46651404-46651423 None:intergenic 35.0%
CAAGGAATAAAATTGCAGAG+AGG + chr2.1:46652141-46652160 MS.gene068879:CDS 35.0%
CAGAAATTCCGTTTGAGAAA+AGG + chr2.1:46651985-46652004 MS.gene068879:CDS 35.0%
CCTGATAATCTTCTATGACA+GGG - chr2.1:46653210-46653229 None:intergenic 35.0%
CTACTATAGAAGAACTTGAG+GGG + chr2.1:46652072-46652091 MS.gene068879:CDS 35.0%
GAAAAGAAGGATTGATGTTG+AGG + chr2.1:46652094-46652113 MS.gene068879:CDS 35.0%
GCAACATCAAAATAACCCAA+AGG - chr2.1:46652014-46652033 None:intergenic 35.0%
TAAAGGAGATGCTATCATGA+TGG + chr2.1:46652430-46652449 MS.gene068879:CDS 35.0%
TAAGATTCCAGTGGTTTCAA+AGG - chr2.1:46653491-46653510 None:intergenic 35.0%
TCAGATTTCCGATCAAGAAT+TGG + chr2.1:46652253-46652272 MS.gene068879:CDS 35.0%
TCATACTCATCCATATCAAC+AGG - chr2.1:46651774-46651793 None:intergenic 35.0%
TCCTGATAATCTTCTATGAC+AGG - chr2.1:46653211-46653230 None:intergenic 35.0%
TCGGCGAATTAACGTAAATA+GGG - chr2.1:46653751-46653770 None:intergenic 35.0%
TCTTCAGCATCTTCTTGAAT+TGG - chr2.1:46652842-46652861 None:intergenic 35.0%
TGATAGAATGGCTAGAGAAA+AGG + chr2.1:46652892-46652911 MS.gene068879:CDS 35.0%
TGATATGGATGAGTATGAGA+AGG + chr2.1:46651776-46651795 MS.gene068879:CDS 35.0%
TGTCATAGAAGATTATCAGG+AGG + chr2.1:46653210-46653229 MS.gene068879:CDS 35.0%
TTCGGCGAATTAACGTAAAT+AGG - chr2.1:46653752-46653771 None:intergenic 35.0%
TTGATAGACCATGTGTGTTA+CGG - chr2.1:46654039-46654058 None:intergenic 35.0%
TTGGATGAAATTGCCAAGTT+GGG + chr2.1:46652272-46652291 MS.gene068879:CDS 35.0%
! ATCTCTTTCCAACTGTTTCT+GGG - chr2.1:46652211-46652230 None:intergenic 35.0%
! CATGATGGAAGCTGAAAATT+TGG + chr2.1:46652445-46652464 MS.gene068879:CDS 35.0%
! TGCATCCTTCAGATTTTTCT+GGG + chr2.1:46652519-46652538 MS.gene068879:CDS 35.0%
! TTGCATCCTTCAGATTTTTC+TGG + chr2.1:46652518-46652537 MS.gene068879:CDS 35.0%
!! TTATTTGCTATCTCCTCAAG+CGG - chr2.1:46653142-46653161 None:intergenic 35.0%
AACTCCTTGATAAGAGCTGA+CGG + chr2.1:46653016-46653035 MS.gene068879:CDS 40.0%
AAGAACTTGAGGGGAAAAGA+AGG + chr2.1:46652081-46652100 MS.gene068879:CDS 40.0%
AAGGACGATAACTATGAACC+TGG + chr2.1:46651678-46651697 MS.gene068879:CDS 40.0%
AAGGTTACATTACTCACGCA+AGG + chr2.1:46654163-46654182 MS.gene068879:CDS 40.0%
ACCCACAATGGTATCACTAA+TGG - chr2.1:46651601-46651620 None:intergenic 40.0%
AGATTCCAGTGGTTTCAAAG+GGG - chr2.1:46653489-46653508 None:intergenic 40.0%
AGCTTTCAGGATTTCATCCT+CGG - chr2.1:46651391-46651410 None:intergenic 40.0%
AGGAGTTCTTAAAGGAGTCA+AGG - chr2.1:46652393-46652412 None:intergenic 40.0%
AGGTTACATTACTCACGCAA+GGG + chr2.1:46654164-46654183 MS.gene068879:CDS 40.0%
AGTCCACTCAGTCTTCTTAA+TGG - chr2.1:46651520-46651539 None:intergenic 40.0%
ATAGCATCTCCTTTACTAGC+TGG - chr2.1:46652425-46652444 None:intergenic 40.0%
ATCCTCTTCAATCTTCTCCT+CGG - chr2.1:46652867-46652886 None:intergenic 40.0%
ATGAAAAAAAGTCTGCGCTC+TGG + chr2.1:46652767-46652786 MS.gene068879:CDS 40.0%
CACTCAGTCTTCTTAATGGA+AGG - chr2.1:46651516-46651535 None:intergenic 40.0%
CCCTGTCATAGAAGATTATC+AGG + chr2.1:46653207-46653226 MS.gene068879:CDS 40.0%
CTCATCCATATCAACAGGAT+CGG - chr2.1:46651769-46651788 None:intergenic 40.0%
CTCCTTTCTTATCATCTCGT+CGG - chr2.1:46653083-46653102 None:intergenic 40.0%
CTTCCATTAAGAAGACTGAG+TGG + chr2.1:46651514-46651533 MS.gene068879:CDS 40.0%
GAATTGGTTGCATCACTATC+TGG - chr2.1:46652826-46652845 None:intergenic 40.0%
GAGCTAGTTGCATATTATGG+TGG + chr2.1:46653570-46653589 MS.gene068879:intron 40.0%
GATCTCTTTCCAACTGTTTC+TGG - chr2.1:46652212-46652231 None:intergenic 40.0%
GCAAATAAGGAGAGAGAGAA+AGG + chr2.1:46653154-46653173 MS.gene068879:CDS 40.0%
GCTTGAGGAGATAGCAAATA+AGG + chr2.1:46653141-46653160 MS.gene068879:CDS 40.0%
GTTATCGTCCTTAACACAAG+TGG - chr2.1:46651670-46651689 None:intergenic 40.0%
TAGAGAAAAGGCAGAAGAAG+AGG + chr2.1:46652904-46652923 MS.gene068879:CDS 40.0%
TCATCCATATCAACAGGATC+GGG - chr2.1:46651768-46651787 None:intergenic 40.0%
TCATTCAAAGGGAACTTCCT+AGG + chr2.1:46652963-46652982 MS.gene068879:CDS 40.0%
TCGTTTGCAAATCTGCAGAA+CGG + chr2.1:46651460-46651479 MS.gene068879:CDS 40.0%
TGAGAAGTAAGCTGGATGAT+GGG + chr2.1:46654113-46654132 MS.gene068879:CDS 40.0%
TGAGAATGTGAGAAGTAAGC+TGG + chr2.1:46654105-46654124 MS.gene068879:CDS 40.0%
TGATAGACCATGTGTGTTAC+GGG - chr2.1:46654038-46654057 None:intergenic 40.0%
TGATGTTGCTGATGAGGATA+GGG + chr2.1:46652025-46652044 MS.gene068879:CDS 40.0%
TGCAGATTTGCAAACGAGTA+GGG - chr2.1:46651457-46651476 None:intergenic 40.0%
TGCTTCAACAAAATCCTCGA+GGG - chr2.1:46653978-46653997 None:intergenic 40.0%
TGGGTGTCTGTGGATAATTA+GGG - chr2.1:46652364-46652383 None:intergenic 40.0%
TGGTTTCAAAGGGGTGAAAA+GGG - chr2.1:46653480-46653499 None:intergenic 40.0%
TTCTTAAAGGAGTCAAGGCT+TGG - chr2.1:46652388-46652407 None:intergenic 40.0%
TTGATGTTGCTGATGAGGAT+AGG + chr2.1:46652024-46652043 MS.gene068879:CDS 40.0%
TTGGGTGTCTGTGGATAATT+AGG - chr2.1:46652365-46652384 None:intergenic 40.0%
TTTGAGAAAAGGCCTCCTTT+GGG + chr2.1:46651996-46652015 MS.gene068879:CDS 40.0%
TTTGATACCCGTAACACACA+TGG + chr2.1:46654028-46654047 MS.gene068879:CDS 40.0%
! AAAGGAGGCCTTTTCTCAAA+CGG - chr2.1:46651996-46652015 None:intergenic 40.0%
! GATGATTCGACTTGAGAAGA+AGG + chr2.1:46654144-46654163 MS.gene068879:CDS 40.0%
! GCAAGGGATGGTTTTTCTTT+TGG + chr2.1:46652644-46652663 MS.gene068879:CDS 40.0%
! GCATCCTTCAGATTTTTCTG+GGG + chr2.1:46652520-46652539 MS.gene068879:CDS 40.0%
! GTTCTCTGAGGAGTTCTTAA+AGG - chr2.1:46652401-46652420 None:intergenic 40.0%
! TCTTTTGGCATGACTACGAA+GGG + chr2.1:46652659-46652678 MS.gene068879:CDS 40.0%
! TGTTCACCTTTTCACCCTTT+GGG + chr2.1:46653333-46653352 MS.gene068879:intron 40.0%
! TTCTTTTGGCATGACTACGA+AGG + chr2.1:46652658-46652677 MS.gene068879:CDS 40.0%
! TTGTTCACCTTTTCACCCTT+TGG + chr2.1:46653332-46653351 MS.gene068879:intron 40.0%
!! ACTCATTTTTAGGCTGTGGA+AGG - chr2.1:46652802-46652821 None:intergenic 40.0%
!! TGGTACTCATTTTTAGGCTG+TGG - chr2.1:46652806-46652825 None:intergenic 40.0%
AAAAGGCAGAAGAAGAGGCA+CGG + chr2.1:46652909-46652928 MS.gene068879:CDS 45.0%
AAGAGAGAATTGAAGGCTGC+TGG + chr2.1:46651912-46651931 MS.gene068879:CDS 45.0%
AATCACGTTGTGTACCCCAA+AGG - chr2.1:46653351-46653370 None:intergenic 45.0%
AATCTGAAGGATGCAACTCC+AGG - chr2.1:46652514-46652533 None:intergenic 45.0%
AATTTCCTAGGGTCATCACC+AGG - chr2.1:46651699-46651718 None:intergenic 45.0%
AGAAACAGCTTGAGGAAGCA+AGG + chr2.1:46651868-46651887 MS.gene068879:CDS 45.0%
AGAGAGAATTGAAGGCTGCT+GGG + chr2.1:46651913-46651932 MS.gene068879:CDS 45.0%
AGCATTGTCATGCTCCAGTA+AGG - chr2.1:46653113-46653132 None:intergenic 45.0%
AGCCGACGAGATGATAAGAA+AGG + chr2.1:46653078-46653097 MS.gene068879:CDS 45.0%
AGGCTGCTGGGATTGATATA+AGG + chr2.1:46651925-46651944 MS.gene068879:CDS 45.0%
AGTATCTTCGTGAGGCAATG+GGG + chr2.1:46653939-46653958 MS.gene068879:CDS 45.0%
ATATGTAGCTCATCCCTGAG+AGG - chr2.1:46652689-46652708 None:intergenic 45.0%
ATCACGTTGTGTACCCCAAA+GGG - chr2.1:46653350-46653369 None:intergenic 45.0%
CAATGCTAAGTATCCGCTTG+AGG + chr2.1:46653126-46653145 MS.gene068879:CDS 45.0%
CAGAGAACACCAGCTAGTAA+AGG + chr2.1:46652413-46652432 MS.gene068879:CDS 45.0%
CAGTATCTTCGTGAGGCAAT+GGG + chr2.1:46653938-46653957 MS.gene068879:CDS 45.0%
CATTGCCTCACGAAGATACT+GGG - chr2.1:46653939-46653958 None:intergenic 45.0%
CTAATAAGCTCCAGAGATGC+TGG - chr2.1:46652995-46653014 None:intergenic 45.0%
CTAATGGTTCTCCACTGAGT+AGG - chr2.1:46651585-46651604 None:intergenic 45.0%
CTGAATGACCCAGAAACAGT+TGG + chr2.1:46652200-46652219 MS.gene068879:CDS 45.0%
CTGCAGATTTGCAAACGAGT+AGG - chr2.1:46651458-46651477 None:intergenic 45.0%
CTGGGATTGATATAAGGCAG+CGG + chr2.1:46651931-46651950 MS.gene068879:CDS 45.0%
GATCCAAACGAGCAGCAAAT+GGG + chr2.1:46653176-46653195 MS.gene068879:CDS 45.0%
GCATGAAAATGATTCCCTCG+AGG + chr2.1:46653961-46653980 MS.gene068879:CDS 45.0%
GGTTTCAAAGGGGTGAAAAG+GGG - chr2.1:46653479-46653498 None:intergenic 45.0%
GTAAAGCTCGTTGGTATGAG+TGG + chr2.1:46651484-46651503 MS.gene068879:CDS 45.0%
GTGACCCCAGAAAAATCTGA+AGG - chr2.1:46652527-46652546 None:intergenic 45.0%
GTGAGAAGTAAGCTGGATGA+TGG + chr2.1:46654112-46654131 MS.gene068879:CDS 45.0%
GTGCTTCAACAAAATCCTCG+AGG - chr2.1:46653979-46653998 None:intergenic 45.0%
GTGGTTTCAAAGGGGTGAAA+AGG - chr2.1:46653481-46653500 None:intergenic 45.0%
GTTTGAGAAAAGGCCTCCTT+TGG + chr2.1:46651995-46652014 MS.gene068879:CDS 45.0%
TCCTCTTCAATCTTCTCCTC+GGG - chr2.1:46652866-46652885 None:intergenic 45.0%
TCTTCTATGACAGGGATAGC+AGG - chr2.1:46653202-46653221 None:intergenic 45.0%
TCTTGATCGGAAATCTGAGG+TGG - chr2.1:46652251-46652270 None:intergenic 45.0%
TGAAAATTTGGCCAGGCTGA+GGG + chr2.1:46652457-46652476 MS.gene068879:CDS 45.0%
TGAGAAAGCAAGACGTTGCA+AGG + chr2.1:46652123-46652142 MS.gene068879:CDS 45.0%
TGGAAGCTGAAAATTTGGCC+AGG + chr2.1:46652450-46652469 MS.gene068879:CDS 45.0%
TGGGGTCACTCCTAAGAAAA+AGG + chr2.1:46652538-46652557 MS.gene068879:CDS 45.0%
TTACTAGCTGGTGTTCTCTG+AGG - chr2.1:46652413-46652432 None:intergenic 45.0%
TTCTGGGTCATTCAGCTTCT+TGG - chr2.1:46652195-46652214 None:intergenic 45.0%
! AAAGGAGTCAAGGCTTGGTT+GGG - chr2.1:46652383-46652402 None:intergenic 45.0%
! CATGGTCTATCAAGTGTTGC+TGG + chr2.1:46654046-46654065 MS.gene068879:CDS 45.0%
! GTTCACCTTTTCACCCTTTG+GGG + chr2.1:46653334-46653353 MS.gene068879:intron 45.0%
! TAAAGGAGTCAAGGCTTGGT+TGG - chr2.1:46652384-46652403 None:intergenic 45.0%
!! AAGTAAGCTGGATGATGGGA+AGG + chr2.1:46654117-46654136 MS.gene068879:CDS 45.0%
!! CAGCATCTCTGGAGCTTATT+AGG + chr2.1:46652993-46653012 MS.gene068879:CDS 45.0%
!! TTTCACCCCTTTGAAACCAC+TGG + chr2.1:46653481-46653500 MS.gene068879:intron 45.0%
!! AAAAATTAAATAGAAAAATT+TGG + chr2.1:46653514-46653533 MS.gene068879:intron 5.0%
!! TTATAATCATTTAATTAATT+AGG + chr2.1:46653632-46653651 MS.gene068879:intron 5.0%
AAAGGAGCTTCCAGCCTTAC+TGG + chr2.1:46653096-46653115 MS.gene068879:CDS 50.0%
AACAGCTTGAGGAAGCAAGG+AGG + chr2.1:46651871-46651890 MS.gene068879:CDS 50.0%
ACCCTAGGAAATTGCGTCCA+GGG + chr2.1:46651706-46651725 MS.gene068879:CDS 50.0%
ACTGTTAGGAGGAGAGAACC+TGG + chr2.1:46652493-46652512 MS.gene068879:CDS 50.0%
AGCTAGGGAGAAACAGCTTG+AGG + chr2.1:46651860-46651879 MS.gene068879:CDS 50.0%
AGGTGTTTGGACAAACACCG+AGG + chr2.1:46651371-46651390 MS.gene068879:CDS 50.0%
AGGTTCTCTCCTCCTAACAG+TGG - chr2.1:46652494-46652513 None:intergenic 50.0%
ATAAGCTCCAGAGATGCTGG+AGG - chr2.1:46652992-46653011 None:intergenic 50.0%
CAGAACGGTGTAAAGCTCGT+TGG + chr2.1:46651475-46651494 MS.gene068879:CDS 50.0%
CCAGTATCTTCGTGAGGCAA+TGG + chr2.1:46653937-46653956 MS.gene068879:CDS 50.0%
CCATTGCCTCACGAAGATAC+TGG - chr2.1:46653940-46653959 None:intergenic 50.0%
CCTTAACACAAGTGGCGTCA+AGG - chr2.1:46651662-46651681 None:intergenic 50.0%
CCTTACTCCCAGAAGTGGTA+TGG + chr2.1:46652616-46652635 MS.gene068879:CDS 50.0%
CCTTGACGCCACTTGTGTTA+AGG + chr2.1:46651659-46651678 MS.gene068879:CDS 50.0%
CTAAGCTTACGCCTACTCAG+TGG + chr2.1:46651571-46651590 MS.gene068879:CDS 50.0%
CTCCTTCGCAGTGTCTTGAA+CGG + chr2.1:46651625-46651644 MS.gene068879:CDS 50.0%
CTGAAAATTTGGCCAGGCTG+AGG + chr2.1:46652456-46652475 MS.gene068879:CDS 50.0%
GAGTCTCAGACACCACTGTT+AGG + chr2.1:46652479-46652498 MS.gene068879:CDS 50.0%
GCAGGCTTCGATTCAGGATT+CGG - chr2.1:46651741-46651760 None:intergenic 50.0%
GGATCCAAACGAGCAGCAAA+TGG + chr2.1:46653175-46653194 MS.gene068879:CDS 50.0%
GGATTGATATAAGGCAGCGG+AGG + chr2.1:46651934-46651953 MS.gene068879:CDS 50.0%
GGTTATGCCAGTGACCTTGT+GGG + chr2.1:46652293-46652312 MS.gene068879:CDS 50.0%
GTCACTGGCATAACCCAACT+TGG - chr2.1:46652288-46652307 None:intergenic 50.0%
GTCTCCGTCAGCTCTTATCA+AGG - chr2.1:46653023-46653042 None:intergenic 50.0%
GTGTACCCCAAAGGGTGAAA+AGG - chr2.1:46653342-46653361 None:intergenic 50.0%
TACCGTTCAAGACACTGCGA+AGG - chr2.1:46651630-46651649 None:intergenic 50.0%
TACGAAGGGAACTCCTCTCA+GGG + chr2.1:46652673-46652692 MS.gene068879:CDS 50.0%
TCTCAGACACCACTGTTAGG+AGG + chr2.1:46652482-46652501 MS.gene068879:CDS 50.0%
TGAAGCCCAGTATCTTCGTG+AGG + chr2.1:46653931-46653950 MS.gene068879:CDS 50.0%
TGAGTTCATTGCTGCCCACA+AGG - chr2.1:46652310-46652329 None:intergenic 50.0%
TGCTTTCCGAAGCATGTGCT+CGG + chr2.1:46651802-46651821 MS.gene068879:CDS 50.0%
TGTTGCTGATGAGGATAGGG+AGG + chr2.1:46652028-46652047 MS.gene068879:CDS 50.0%
TTGATATAAGGCAGCGGAGG+AGG + chr2.1:46651937-46651956 MS.gene068879:CDS 50.0%
TTGGCATGCAGAATAGCAGC+CGG - chr2.1:46652176-46652195 None:intergenic 50.0%
TTGTCATGCTCCAGTAAGGC+TGG - chr2.1:46653109-46653128 None:intergenic 50.0%
! ATGAACCTGGTGATGACCCT+AGG + chr2.1:46651691-46651710 MS.gene068879:CDS 50.0%
! CCATACCACTTCTGGGAGTA+AGG - chr2.1:46652619-46652638 None:intergenic 50.0%
! TGATCGGAAATCTGAGGTGG+TGG - chr2.1:46652248-46652267 None:intergenic 50.0%
!! ATCTCGTCGGCTTGCTCAAT+AGG - chr2.1:46653070-46653089 None:intergenic 50.0%
!! TCTCGTCGGCTTGCTCAATA+GGG - chr2.1:46653069-46653088 None:intergenic 50.0%
ACGAGTAGCACTGCTGCCTT+CGG - chr2.1:46652333-46652352 None:intergenic 55.0%
AGAAGATGCTGAAGAGCCCG+AGG + chr2.1:46652847-46652866 MS.gene068879:CDS 55.0%
AGCCAACCGAGCACATGCTT+CGG - chr2.1:46651811-46651830 None:intergenic 55.0%
AGCGGAGGAGGAAAAGGAGA+GGG + chr2.1:46651949-46651968 MS.gene068879:CDS 55.0%
AGTAAGGCCTGCACTTCCAG+GGG - chr2.1:46652603-46652622 None:intergenic 55.0%
ATTGCTGCCCACAAGGTCAC+TGG - chr2.1:46652303-46652322 None:intergenic 55.0%
CCCCTGGACGCAATTTCCTA+GGG - chr2.1:46651710-46651729 None:intergenic 55.0%
CCCTAGGAAATTGCGTCCAG+GGG + chr2.1:46651707-46651726 MS.gene068879:CDS 55.0%
CTACGAAGGGAACTCCTCTC+AGG + chr2.1:46652672-46652691 MS.gene068879:CDS 55.0%
GACCCTAGGAAATTGCGTCC+AGG + chr2.1:46651705-46651724 MS.gene068879:CDS 55.0%
GAGTAAGGCCTGCACTTCCA+GGG - chr2.1:46652604-46652623 None:intergenic 55.0%
GCCCGAGGAGAAGATTGAAG+AGG + chr2.1:46652862-46652881 MS.gene068879:CDS 55.0%
GCGACCCGATCCTGTTGATA+TGG + chr2.1:46651761-46651780 MS.gene068879:CDS 55.0%
GCTTGCTCAATAGGGGTTGG+TGG - chr2.1:46653061-46653080 None:intergenic 55.0%
GGATTCGGATCAATCTCCCC+TGG - chr2.1:46651726-46651745 None:intergenic 55.0%
GGCAGCAATGAACTCACCGA+AGG + chr2.1:46652314-46652333 MS.gene068879:CDS 55.0%
GGGTTATGCCAGTGACCTTG+TGG + chr2.1:46652292-46652311 MS.gene068879:CDS 55.0%
TAAGGCAGCGGAGGAGGAAA+AGG + chr2.1:46651943-46651962 MS.gene068879:CDS 55.0%
TCCCCTGGACGCAATTTCCT+AGG - chr2.1:46651711-46651730 None:intergenic 55.0%
TGCTGATGAGGATAGGGAGG+TGG + chr2.1:46652031-46652050 MS.gene068879:CDS 55.0%
TGGCATGCAGAATAGCAGCC+GGG - chr2.1:46652175-46652194 None:intergenic 55.0%
TTCCGAAGCATGTGCTCGGT+TGG + chr2.1:46651806-46651825 MS.gene068879:CDS 55.0%
TTGCAGAGGGAGTCAGCAGT+GGG - chr2.1:46652580-46652599 None:intergenic 55.0%
! AAGGCTTGGTTGGGTGTCTG+TGG - chr2.1:46652374-46652393 None:intergenic 55.0%
! AGAAGAAGAGGCACGGCAGA+AGG + chr2.1:46652916-46652935 MS.gene068879:CDS 55.0%
! AGGCCCATTTGCTGCTCGTT+TGG - chr2.1:46653182-46653201 None:intergenic 55.0%
! CGAAGGAGTACGACCCACAA+TGG - chr2.1:46651613-46651632 None:intergenic 55.0%
! GACTGAGTGGACTCGAGAAG+AGG + chr2.1:46651527-46651546 MS.gene068879:CDS 55.0%
! GCTCGGTTGGCTAACACGAA+AGG + chr2.1:46651819-46651838 MS.gene068879:CDS 55.0%
! TCGGCTTGCTCAATAGGGGT+TGG - chr2.1:46653064-46653083 None:intergenic 55.0%
!! AAGTGGTATGGCGCTGGCAA+GGG + chr2.1:46652628-46652647 MS.gene068879:CDS 55.0%
!! CTCGTCGGCTTGCTCAATAG+GGG - chr2.1:46653068-46653087 None:intergenic 55.0%
AGAGATGCTGGAGGAGGCCT+AGG - chr2.1:46652983-46653002 None:intergenic 60.0%
AGCTCCAGAGATGCTGGAGG+AGG - chr2.1:46652989-46653008 None:intergenic 60.0%
CAGCGGAGGAGGAAAAGGAG+AGG + chr2.1:46651948-46651967 MS.gene068879:CDS 60.0%
GAGGGAGTCAGCAGTGGGAT+TGG - chr2.1:46652575-46652594 None:intergenic 60.0%
GCACTTCCAGGGGTTGCAGA+GGG - chr2.1:46652593-46652612 None:intergenic 60.0%
GCAGGCCTTACTCCCAGAAG+TGG + chr2.1:46652611-46652630 MS.gene068879:CDS 60.0%
GCCAGCGCCATACCACTTCT+GGG - chr2.1:46652626-46652645 None:intergenic 60.0%
GGAGTAAGGCCTGCACTTCC+AGG - chr2.1:46652605-46652624 None:intergenic 60.0%
GGCATGCAGAATAGCAGCCG+GGG - chr2.1:46652174-46652193 None:intergenic 60.0%
GTTGCAGAGGGAGTCAGCAG+TGG - chr2.1:46652581-46652600 None:intergenic 60.0%
TAGGCCTCCTCCAGCATCTC+TGG + chr2.1:46652982-46653001 MS.gene068879:CDS 60.0%
TCCCAGAAGTGGTATGGCGC+TGG + chr2.1:46652622-46652641 MS.gene068879:CDS 60.0%
TGCACTTCCAGGGGTTGCAG+AGG - chr2.1:46652594-46652613 None:intergenic 60.0%
TGCAGAGAGGCAAGATGCCC+CGG + chr2.1:46652154-46652173 MS.gene068879:CDS 60.0%
TGCCAGCGCCATACCACTTC+TGG - chr2.1:46652627-46652646 None:intergenic 60.0%
! TCTGCAACCCCTGGAAGTGC+AGG + chr2.1:46652593-46652612 MS.gene068879:CDS 60.0%
! TGTCTGAGACTCCCTCAGCC+TGG - chr2.1:46652471-46652490 None:intergenic 60.0%
!! GAAGTGGTATGGCGCTGGCA+AGG + chr2.1:46652627-46652646 MS.gene068879:CDS 60.0%
CTGACTCCCTCTGCAACCCC+TGG + chr2.1:46652584-46652603 MS.gene068879:CDS 65.0%
GGTCGCGCAGGCTTCGATTC+AGG - chr2.1:46651747-46651766 None:intergenic 65.0%
TCAACAGGATCGGGTCGCGC+AGG - chr2.1:46651759-46651778 None:intergenic 65.0%
!! GGTATGGCGCTGGCAAGGGA+TGG + chr2.1:46652632-46652651 MS.gene068879:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 46651363 46654207 46651363 ID=MS.gene068879
chr2.1 mRNA 46651363 46654207 46651363 ID=MS.gene068879.t1;Parent=MS.gene068879
chr2.1 exon 46651363 46653246 46651363 ID=MS.gene068879.t1.exon1;Parent=MS.gene068879.t1
chr2.1 CDS 46651363 46653246 46651363 ID=cds.MS.gene068879.t1;Parent=MS.gene068879.t1
chr2.1 exon 46653911 46654207 46653911 ID=MS.gene068879.t1.exon2;Parent=MS.gene068879.t1
chr2.1 CDS 46653911 46654207 46653911 ID=cds.MS.gene068879.t1;Parent=MS.gene068879.t1
Gene Sequence

>MS.gene068879

ATGAGCATAGGTGTTTGGACAAACACCGAGGATGAAATCCTGAAAGCTGCTGTTATGAAATATGGTAAAAATCAGTGGGCTCATATCTCGTCCCTACTCGTTTGCAAATCTGCAGAACGGTGTAAAGCTCGTTGGTATGAGTGGTTAGATCCTTCCATTAAGAAGACTGAGTGGACTCGAGAAGAGGATGAGAAGCTACTTCATCTTGCTAAGCTTACGCCTACTCAGTGGAGAACCATTAGTGATACCATTGTGGGTCGTACTCCTTCGCAGTGTCTTGAACGGTATGAGAAACTCCTTGACGCCACTTGTGTTAAGGACGATAACTATGAACCTGGTGATGACCCTAGGAAATTGCGTCCAGGGGAGATTGATCCGAATCCTGAATCGAAGCCTGCGCGACCCGATCCTGTTGATATGGATGAGTATGAGAAGGAGATGCTTTCCGAAGCATGTGCTCGGTTGGCTAACACGAAAGGTAAAAAAGCAAAAAGGAAAGCTAGGGAGAAACAGCTTGAGGAAGCAAGGAGGCTTGCTTCTTTACAGAAAAAGAGAGAATTGAAGGCTGCTGGGATTGATATAAGGCAGCGGAGGAGGAAAAGGAGAGGGATTGATTATAATGCAGAAATTCCGTTTGAGAAAAGGCCTCCTTTGGGTTATTTTGATGTTGCTGATGAGGATAGGGAGGTGGAGCAGCATGCATTCCCTACTACTATAGAAGAACTTGAGGGGAAAAGAAGGATTGATGTTGAGGCTCAGTTGAGAAAGCAAGACGTTGCAAGGAATAAAATTGCAGAGAGGCAAGATGCCCCGGCTGCTATTCTGCATGCCAAGAAGCTGAATGACCCAGAAACAGTTGGAAAGAGATCAAAACTGATGCTTCCACCACCTCAGATTTCCGATCAAGAATTGGATGAAATTGCCAAGTTGGGTTATGCCAGTGACCTTGTGGGCAGCAATGAACTCACCGAAGGCAGCAGTGCTACTCGTGCTCTTCTCCCTAATTATCCACAGACACCCAACCAAGCCTTGACTCCTTTAAGAACTCCTCAGAGAACACCAGCTAGTAAAGGAGATGCTATCATGATGGAAGCTGAAAATTTGGCCAGGCTGAGGGAGTCTCAGACACCACTGTTAGGAGGAGAGAACCTGGAGTTGCATCCTTCAGATTTTTCTGGGGTCACTCCTAAGAAAAAGGAAATTCACATTCCAATCCCACTGCTGACTCCCTCTGCAACCCCTGGAAGTGCAGGCCTTACTCCCAGAAGTGGTATGGCGCTGGCAAGGGATGGTTTTTCTTTTGGCATGACTACGAAGGGAACTCCTCTCAGGGATGAGCTACATATCAATGAAGATATGGAGATGCGTGATAGTGCTAGACTCGAGCTACAGAGACAAGCTGATATGAAAAAAAGTCTGCGCTCTGGTTTAAGCAGCCTTCCACAGCCTAAAAATGAGTACCAGATAGTGATGCAACCAATTCAAGAAGATGCTGAAGAGCCCGAGGAGAAGATTGAAGAGGATATGTCTGATAGAATGGCTAGAGAAAAGGCAGAAGAAGAGGCACGGCAGAAGGCGTTACTTAGAAAACGATCAAAAGTCATTCAAAGGGAACTTCCTAGGCCTCCTCCAGCATCTCTGGAGCTTATTAGGAACTCCTTGATAAGAGCTGACGGAGACAAAAGTTCATTTGTTCCACCAACCCCTATTGAGCAAGCCGACGAGATGATAAGAAAGGAGCTTCCAGCCTTACTGGAGCATGACAATGCTAAGTATCCGCTTGAGGAGATAGCAAATAAGGAGAGAGAGAAAGGATCCAAACGAGCAGCAAATGGGCCTGCTATCCCTGTCATAGAAGATTATCAGGAGGACGAGATGAAATCTGTATGTTTTTACTAATTTATCTGCTCACCTTTAATTTTATTTTTATGCCTTTTACTTCGCTTTTCTTCATTTGCTACTAAGTAGCTTGTTCACCTTTTCACCCTTTGGGGTACACAACGTGATTTTTCATTTAGATGATACACATGCTTTTTCTGATGATCTTGATCTTGAAATTATTAGTACTTTGTCCGATTATTAATTACTTGGCATCATCATTTTTATGCTGAAACCCCTTTTCACCCCTTTGAAACCACTGGAATCTTATGTAAAAATTAAATAGAAAAATTTGGACATCTTTTTAATTTATGTATCATCTCCTTTATGAGCTAGTTGCATATTATGGTGGTATGTAATATGTATTGTATCGTCCTTGTTTTTTTTTCAGTTATAATCATTTAATTAATTAGGTTGAAGTTAGTTTTAGTTTCTCGCAATTGTTGTTCTCTATTTCTTTCGTTTTTGTAACAAGTGATTCTTCAATTGCCTTTTACAACATTTATTCCCTATTTACGTTAATTCGCCGAATGATAGTAATACAAACTTCCAAACTGCTTGTTAATTATTATTTGACTCCTTAAATTTGACTATGGAGATGAGCTTGTACGTTGATGAATGATAATATCTGTGTTGACTGTTATTTATTTTTATCTGTTGTTCTCAATAGGCTGATCAGTTGATAAAAGATGAAGCCCAGTATCTTCGTGAGGCAATGGGGCATGAAAATGATTCCCTCGAGGATTTTGTTGAAGCACATACAACATGCATTAATGATTTAATGTACTTTGATACCCGTAACACACATGGTCTATCAAGTGTTGCTGGAAACGCAGAGAAGCTAGCAGCTTTGCAGAATGAATTTGAGAATGTGAGAAGTAAGCTGGATGATGGGAAGGAGAAGATGATTCGACTTGAGAAGAAGGTTACATTACTCACGCAAGGGTACGAGTTATGCTACTGCTAA
Protein sequence

>MS.gene068879.t1

MSIGVWTNTEDEILKAAVMKYGKNQWAHISSLLVCKSAERCKARWYEWLDPSIKKTEWTREEDEKLLHLAKLTPTQWRTISDTIVGRTPSQCLERYEKLLDATCVKDDNYEPGDDPRKLRPGEIDPNPESKPARPDPVDMDEYEKEMLSEACARLANTKGKKAKRKAREKQLEEARRLASLQKKRELKAAGIDIRQRRRKRRGIDYNAEIPFEKRPPLGYFDVADEDREVEQHAFPTTIEELEGKRRIDVEAQLRKQDVARNKIAERQDAPAAILHAKKLNDPETVGKRSKLMLPPPQISDQELDEIAKLGYASDLVGSNELTEGSSATRALLPNYPQTPNQALTPLRTPQRTPASKGDAIMMEAENLARLRESQTPLLGGENLELHPSDFSGVTPKKKEIHIPIPLLTPSATPGSAGLTPRSGMALARDGFSFGMTTKGTPLRDELHINEDMEMRDSARLELQRQADMKKSLRSGLSSLPQPKNEYQIVMQPIQEDAEEPEEKIEEDMSDRMAREKAEEEARQKALLRKRSKVIQRELPRPPPASLELIRNSLIRADGDKSSFVPPTPIEQADEMIRKELPALLEHDNAKYPLEEIANKEREKGSKRAANGPAIPVIEDYQEDEMKSADQLIKDEAQYLREAMGHENDSLEDFVEAHTTCINDLMYFDTRNTHGLSSVAGNAEKLAALQNEFENVRSKLDDGKEKMIRLEKKVTLLTQGYELCYC