AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053100.t1 MTR_6g004130 90.868 219 12 2 1 219 1 211 8.07e-123 347
MS.gene053100.t1 MTR_4g035350 51.908 131 60 2 85 214 56 184 8.54e-44 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053100.t1 AT5G47870 76.351 148 31 2 66 213 56 199 1.88e-80 239
MS.gene053100.t1 AT1G71310 50.382 131 61 2 85 214 47 174 2.06e-42 141
MS.gene053100.t1 AT1G71310 50.382 131 61 2 85 214 47 174 2.06e-42 141
MS.gene053100.t1 AT1G71310 44.318 88 45 2 85 171 47 131 3.18e-21 86.7

Find 45 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGGTTTAACGAGAGTATC+AGG 0.259720 6.2:-142107 None:intergenic
TATCAGGGATTCGCTTATTA+AGG 0.275884 6.2:-142091 None:intergenic
GAAGAAGAAGCGGAGGAATT+AGG 0.288624 6.2:-142137 None:intergenic
TTGATAGGTATCATGCTAAT+AGG 0.296623 6.2:+142452 MS.gene053100:intron
TTCAAATTATGTTGTACCTT+TGG 0.309936 6.2:+142001 MS.gene053100:CDS
CAATTGAACCATCATCAATA+TGG 0.328537 6.2:+141865 MS.gene053100:CDS
CTTCTTCCACCTTCATCCCC+TGG 0.368735 6.2:+142153 MS.gene053100:CDS
GCATGTGCGCGGTTTGGTCT+TGG 0.393154 6.2:+145557 MS.gene053100:CDS
CTTGTAAACCATATTGATGA+TGG 0.407031 6.2:-141873 None:intergenic
AAAGATCGGTTCTTACATAC+TGG 0.409720 6.2:-145609 None:intergenic
GGAAAGAAAGGAGGAGGTGT+AGG 0.419430 6.2:+141963 MS.gene053100:CDS
TGTAAAGCATGTGCGCGGTT+TGG 0.440363 6.2:+145551 MS.gene053100:CDS
TGCTGAAACTGGATCACTAA+TGG 0.460299 6.2:-145510 None:intergenic
AAGAAAGGAGGAGGTGTAGG+AGG 0.464745 6.2:+141966 MS.gene053100:CDS
GATGAAGAAGAAGAAGTGAA+TGG 0.465006 6.2:-141825 None:intergenic
CAGGCCTATCGTGAGTCAAC+CGG 0.466364 6.2:+145464 MS.gene053100:intron
AGCAGCAGCAATAGCAGTGA+TGG 0.477522 6.2:+141942 MS.gene053100:CDS
CTTACTGTACGCGGATCTGA+TGG 0.479382 6.2:+142631 MS.gene053100:CDS
AATAGCAGTGATGGAAAGAA+AGG 0.490604 6.2:+141951 MS.gene053100:CDS
ACAAGAAAGACGAGAAGTAT+TGG 0.497859 6.2:+141891 MS.gene053100:CDS
TCTCTAAGAATCTCAGCTAA+AGG 0.509951 6.2:-142068 None:intergenic
ATGTTAAGCTTCTATGCTCC+AGG 0.515074 6.2:+142475 MS.gene053100:CDS
GATGAAGGTGGAAGAAGAAG+CGG 0.516992 6.2:-142147 None:intergenic
AAGTACTGACCAGGGGATGA+AGG 0.517778 6.2:-142162 None:intergenic
AGTGATGGAAAGAAAGGAGG+AGG 0.521198 6.2:+141957 MS.gene053100:CDS
ATCAGTAGTTGATATTGTGC+CGG 0.531881 6.2:-145483 None:intergenic
CTCAGCTAAAGGACGAGTAA+TGG 0.549865 6.2:-142057 None:intergenic
AGCGGAGGAATTAGGATCGG+TGG 0.551381 6.2:-142129 None:intergenic
GAAGGTGGAAGAAGAAGCGG+AGG 0.575926 6.2:-142144 None:intergenic
TGATCCAGTTTCAGCAGCGG+AGG 0.596606 6.2:+145517 MS.gene053100:CDS
GCGGAGGAATTAGGATCGGT+GGG 0.596981 6.2:-142128 None:intergenic
GGCACAATATCAACTACTGA+TGG 0.599190 6.2:+145485 MS.gene053100:CDS
AGCTAAAGGACGAGTAATGG+TGG 0.602256 6.2:-142054 None:intergenic
TACTGACCAGGGGATGAAGG+TGG 0.628798 6.2:-142159 None:intergenic
GAGGTTTAACGAGAGTATCA+GGG 0.629655 6.2:-142106 None:intergenic
AATGAAAATGAATCATCCAA+AGG 0.640887 6.2:-142017 None:intergenic
TAGTGATCCAGTTTCAGCAG+CGG 0.651588 6.2:+145514 MS.gene053100:CDS
CAGATCCGCGTACAGTAAGA+CGG 0.655531 6.2:-142627 None:intergenic
AGAAGCGGAGGAATTAGGAT+CGG 0.656165 6.2:-142132 None:intergenic
AGCAGTGATGGAAAGAAAGG+AGG 0.660145 6.2:+141954 MS.gene053100:CDS
GAGGAATTAGGATCGGTGGG+AGG 0.660294 6.2:-142125 None:intergenic
TGTGCCGGTTGACTCACGAT+AGG 0.667563 6.2:-145468 None:intergenic
CTTTCTGTAAAGCATGTGCG+CGG 0.675897 6.2:+145546 MS.gene053100:CDS
TGTACGCGGATCTGATGGAG+AGG 0.690894 6.2:+142636 MS.gene053100:CDS
GTATACCGTCTTACTGTACG+CGG 0.755443 6.2:+142622 MS.gene053100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTTATAAACATTATAT+AGG + chr6.2:143000-143019 MS.gene053100:intron 10.0%
!! ATATAATGTTTATAAAGTTT+GGG - chr6.2:143000-143019 None:intergenic 10.0%
!! TATATAATGTTTATAAAGTT+TGG - chr6.2:143001-143020 None:intergenic 10.0%
!!! TTTTATTATTTTTTTGCTAT+TGG - chr6.2:144877-144896 None:intergenic 10.0%
!! ACATTGAATTGAATATAATT+TGG + chr6.2:145078-145097 MS.gene053100:intron 15.0%
!! CAGATTTAAATCATAATAAT+GGG - chr6.2:142836-142855 None:intergenic 15.0%
!! GAAATTATAGATGAATTAAA+GGG + chr6.2:144940-144959 MS.gene053100:intron 15.0%
!! TCAGATTTAAATCATAATAA+TGG - chr6.2:142837-142856 None:intergenic 15.0%
!! TGAAATTATAGATGAATTAA+AGG + chr6.2:144939-144958 MS.gene053100:intron 15.0%
!! TTAATTCATCTATAATTTCA+AGG - chr6.2:144939-144958 None:intergenic 15.0%
!!! AATTTAGATAGTTTTGAATT+TGG + chr6.2:142528-142547 MS.gene053100:intron 15.0%
!!! TTGATAATTTAAGAGATTTT+TGG - chr6.2:144755-144774 None:intergenic 15.0%
!! AACTTAAATCAATATTACTC+TGG - chr6.2:145222-145241 None:intergenic 20.0%
!! AATAAGAAGAGAGAAAAATA+AGG - chr6.2:144025-144044 None:intergenic 20.0%
!! CTTTAAATCATAATGAGAAA+TGG - chr6.2:144139-144158 None:intergenic 20.0%
!! TAATAAAATGAAAATGAACG+TGG + chr6.2:144889-144908 MS.gene053100:intron 20.0%
!! TATGTTAAATATACTTGCAT+TGG + chr6.2:143357-143376 MS.gene053100:intron 20.0%
!! TATTAGATTCAATATGATCT+AGG + chr6.2:142863-142882 MS.gene053100:intron 20.0%
!! TCATTTCTCAATCATAATAA+CGG - chr6.2:143965-143984 None:intergenic 20.0%
!! TTACTAGCATATATAATTGT+TGG - chr6.2:143142-143161 None:intergenic 20.0%
!! TTCAATGTAATAATGTTCAA+AGG - chr6.2:145066-145085 None:intergenic 20.0%
!!! AACCAAAATTCTAAGAATAA+TGG - chr6.2:142932-142951 None:intergenic 20.0%
!!! AATAGAATATAATTTGCCAA+AGG + chr6.2:144178-144197 MS.gene053100:intron 20.0%
!!! AATGTTTTTGATAAGATTAG+TGG - chr6.2:144262-144281 None:intergenic 20.0%
!!! AATTTGGAATATGTTTTTGT+AGG + chr6.2:142544-142563 MS.gene053100:intron 20.0%
!!! TTATTTTTCTCTCTTCTTAT+TGG + chr6.2:144024-144043 MS.gene053100:intron 20.0%
! AAAATACATTCTGTGCAAAT+TGG + chr6.2:143855-143874 MS.gene053100:intron 25.0%
! AAACATGATAGATTAGATGA+GGG - chr6.2:142668-142687 None:intergenic 25.0%
! AAATTTCAAATCAGAACGAT+GGG - chr6.2:143422-143441 None:intergenic 25.0%
! AAGATTGCTAGCATATATAA+TGG - chr6.2:143518-143537 None:intergenic 25.0%
! AAGTTTCTTATGTCTGTTAT+TGG + chr6.2:143543-143562 MS.gene053100:intron 25.0%
! AATGAAAATGAATCATCCAA+AGG - chr6.2:142020-142039 None:intergenic 25.0%
! AATGGAATAGTAGAAAGTTA+GGG - chr6.2:143248-143267 None:intergenic 25.0%
! ATTATCAACTATGTAGATGT+TGG + chr6.2:143644-143663 MS.gene053100:intron 25.0%
! CATGTGATTAAGTTCAATTA+TGG + chr6.2:142279-142298 MS.gene053100:intron 25.0%
! CATGTTAAATAAACTTGCAT+TGG + chr6.2:144467-144486 MS.gene053100:intron 25.0%
! CCAAAACAATAGATAGAAAT+AGG + chr6.2:144048-144067 MS.gene053100:intron 25.0%
! GAAAATGTCCAATATGATTA+TGG + chr6.2:145100-145119 MS.gene053100:intron 25.0%
! GAATGCATTAGAAGTAAAAT+AGG - chr6.2:144972-144991 None:intergenic 25.0%
! TAAACATGATAGATTAGATG+AGG - chr6.2:142669-142688 None:intergenic 25.0%
! TGATTAGTGGCATATATAAA+AGG - chr6.2:145155-145174 None:intergenic 25.0%
! TTACTCTGGATTTAGATTTA+TGG - chr6.2:145208-145227 None:intergenic 25.0%
! TTCAAATTATGTTGTACCTT+TGG + chr6.2:142001-142020 MS.gene053100:CDS 25.0%
!! AACTATTTTGTGACAACTTT+TGG + chr6.2:143994-144013 MS.gene053100:intron 25.0%
!! ACAAAGAAAGTTAGTCATTT+TGG - chr6.2:145438-145457 None:intergenic 25.0%
!! ACTATTTTGTGACAACTTTT+GGG + chr6.2:143995-144014 MS.gene053100:intron 25.0%
!! TGAAAAGTTCTGTGATTTTT+CGG - chr6.2:144360-144379 None:intergenic 25.0%
!! TGACTCTGAATTTAGATTTT+GGG - chr6.2:143198-143217 None:intergenic 25.0%
!! TGACTCTGATTTTAGATTAA+GGG - chr6.2:144687-144706 None:intergenic 25.0%
!! TGCCATTATTCTTAGAATTT+TGG + chr6.2:142927-142946 MS.gene053100:intron 25.0%
!!! AAGCTTTAAATCAAAATGAG+GGG - chr6.2:144529-144548 None:intergenic 25.0%
!!! AGAAGCTTTAAATCAAAATG+AGG - chr6.2:144531-144550 None:intergenic 25.0%
!!! AGTTGATAATTTGAGGTTTT+GGG - chr6.2:143634-143653 None:intergenic 25.0%
!!! CCTATTTCTATCTATTGTTT+TGG - chr6.2:144051-144070 None:intergenic 25.0%
!!! GAAGCTTTAAATCAAAATGA+GGG - chr6.2:144530-144549 None:intergenic 25.0%
!!! GATGTTATTTTCTCTGATAA+TGG + chr6.2:142586-142605 MS.gene053100:CDS 25.0%
!!! TAGTTGATAATTTGAGGTTT+TGG - chr6.2:143635-143654 None:intergenic 25.0%
!!! TCTACATAGTTGATAATTTG+AGG - chr6.2:143641-143660 None:intergenic 25.0%
AAAAAACAGAAGAATAGAGG+TGG - chr6.2:143828-143847 None:intergenic 30.0%
AAAGGACAGAAAAAGAATAG+AGG - chr6.2:145013-145032 None:intergenic 30.0%
AAATGTGGTTAGAGAGAAAA+AGG - chr6.2:145031-145050 None:intergenic 30.0%
AATGTGGTTCAGAAGAAAAA+AGG - chr6.2:143307-143326 None:intergenic 30.0%
AGATTAGTGGCACATATAAA+AGG - chr6.2:144249-144268 None:intergenic 30.0%
AGATTATTAGCGATTTCACA+CGG - chr6.2:143733-143752 None:intergenic 30.0%
AGGTAGCAAATATGAATGAA+TGG - chr6.2:143266-143285 None:intergenic 30.0%
ATATTCAAAGTCCAATATGC+AGG - chr6.2:143331-143350 None:intergenic 30.0%
ATCCACTTAGAATCAAACAT+AGG - chr6.2:144383-144402 None:intergenic 30.0%
ATGCACTTAGAATCAAACAT+AGG - chr6.2:145299-145318 None:intergenic 30.0%
ATGTCCAATATGATTATGGT+AGG + chr6.2:145104-145123 MS.gene053100:intron 30.0%
ATTGCACTTTCTGAACTTAT+TGG + chr6.2:143909-143928 MS.gene053100:intron 30.0%
CAATTGAACCATCATCAATA+TGG + chr6.2:141865-141884 MS.gene053100:CDS 30.0%
CATGTTAAATAAACTCGCAT+TGG + chr6.2:142775-142794 MS.gene053100:intron 30.0%
CGTTAGATTTGATATGATCT+AGG + chr6.2:143449-143468 MS.gene053100:intron 30.0%
CTTGTAAACCATATTGATGA+TGG - chr6.2:141876-141895 None:intergenic 30.0%
CTTTCTGAACTTATTGGAAT+TGG + chr6.2:143915-143934 MS.gene053100:intron 30.0%
GAAATTTCAAATCAGAACGA+TGG - chr6.2:143423-143442 None:intergenic 30.0%
GAATGGAATAGTAGAAAGTT+AGG - chr6.2:143249-143268 None:intergenic 30.0%
GGAAAAAAACAGAAGAATAG+AGG - chr6.2:143831-143850 None:intergenic 30.0%
GGTTAGTGGCATTATATAAA+TGG - chr6.2:144625-144644 None:intergenic 30.0%
TAGATTCAATATGATCTAGG+TGG + chr6.2:142866-142885 MS.gene053100:intron 30.0%
TAGATTTGATATGATCTAGG+CGG + chr6.2:143452-143471 MS.gene053100:intron 30.0%
TCGAAAATAAGCCAATCAAA+GGG - chr6.2:142307-142326 None:intergenic 30.0%
TGACTAACTTTCTTTGTTGA+TGG + chr6.2:145441-145460 MS.gene053100:intron 30.0%
TGACTCCGAATTTAAATGTA+GGG - chr6.2:143570-143589 None:intergenic 30.0%
TGACTCTGGATTTAAATTCA+GGG - chr6.2:144292-144311 None:intergenic 30.0%
TGATTGCATTAGAAGTTAGA+AGG - chr6.2:143793-143812 None:intergenic 30.0%
TTATCTGCTTGTTCTTTGAT+AGG + chr6.2:142437-142456 MS.gene053100:intron 30.0%
TTATGTAAAAGAGCTTGGTT+TGG + chr6.2:142329-142348 MS.gene053100:intron 30.0%
TTGATAGGTATCATGCTAAT+AGG + chr6.2:142452-142471 MS.gene053100:intron 30.0%
TTGGACCCTACATTTAAATT+CGG + chr6.2:143562-143581 MS.gene053100:intron 30.0%
TTGGGATTAAATAGTGTAGA+TGG - chr6.2:143616-143635 None:intergenic 30.0%
! CAAACTTTTGATAAGGTTAG+TGG - chr6.2:144639-144658 None:intergenic 30.0%
! CACATGACAAACTTTTGATA+AGG - chr6.2:144646-144665 None:intergenic 30.0%
! CTGACTCTGAATTTAGATTT+TGG - chr6.2:143199-143218 None:intergenic 30.0%
! CTGACTCTGATTTTAGATTA+AGG - chr6.2:144688-144707 None:intergenic 30.0%
!! TGCACAGAATGTATTTTAGA+AGG - chr6.2:143852-143871 None:intergenic 30.0%
!! TGCTTCTAAAACACATTATG+TGG + chr6.2:142725-142744 MS.gene053100:intron 30.0%
!! TGGAATATGTTTTTGTAGGA+TGG + chr6.2:142548-142567 MS.gene053100:intron 30.0%
!!! AATGTGTTTTAGAAGCAAGA+AGG - chr6.2:142722-142741 None:intergenic 30.0%
AAAGCATGTGTGAAAGAATG+TGG + chr6.2:143706-143725 MS.gene053100:intron 35.0%
ACAGCCTACCATAATCATAT+TGG - chr6.2:145111-145130 None:intergenic 35.0%
ACTACACTACAACAACACAA+GGG - chr6.2:144790-144809 None:intergenic 35.0%
ACTTACAAACCAGTTTGGTA+AGG - chr6.2:142968-142987 None:intergenic 35.0%
AGAAAAAGAATAGAGGTAGG+TGG - chr6.2:145006-145025 None:intergenic 35.0%
AGATTTGATATGATCTAGGC+GGG + chr6.2:143453-143472 MS.gene053100:intron 35.0%
ATAACTCAACCTTACCAAAC+TGG + chr6.2:142956-142975 MS.gene053100:intron 35.0%
ATATCTGAGAACGAATGCAA+AGG + chr6.2:145348-145367 MS.gene053100:intron 35.0%
ATGATAGATTAGATGAGGGT+AGG - chr6.2:142664-142683 None:intergenic 35.0%
CACCTATGTTTGATTCTAAG+TGG + chr6.2:144378-144397 MS.gene053100:intron 35.0%
CTATTCAAGCCAAAAATCCA+CGG + chr6.2:145264-145283 MS.gene053100:intron 35.0%
CTCGAAAATAAGCCAATCAA+AGG - chr6.2:142308-142327 None:intergenic 35.0%
CTGAATTTAAATCCAGAGTC+AGG + chr6.2:144291-144310 MS.gene053100:intron 35.0%
CTGACTCCGAATTTAAATGT+AGG - chr6.2:143571-143590 None:intergenic 35.0%
CTGACTCTGGATTTAAATTC+AGG - chr6.2:144293-144312 None:intergenic 35.0%
GACAGAAAAAGAATAGAGGT+AGG - chr6.2:145009-145028 None:intergenic 35.0%
GATGAAGAAGAAGAAGTGAA+TGG - chr6.2:141828-141847 None:intergenic 35.0%
GATTATTAGCGATTTCACAC+GGG - chr6.2:143732-143751 None:intergenic 35.0%
GCTTCTGAGTGATATTGTAT+CGG + chr6.2:142898-142917 MS.gene053100:intron 35.0%
TAGTCTTCGATCAGAAATGT+CGG - chr6.2:142228-142247 None:intergenic 35.0%
TATCAGGGATTCGCTTATTA+AGG - chr6.2:142094-142113 None:intergenic 35.0%
TCTGAACTTATTGGAATTGG+AGG + chr6.2:143918-143937 MS.gene053100:intron 35.0%
! AATAGCAGTGATGGAAAGAA+AGG + chr6.2:141951-141970 MS.gene053100:CDS 35.0%
! ACAAGAAAGACGAGAAGTAT+TGG + chr6.2:141891-141910 MS.gene053100:CDS 35.0%
! AGGCTAATTTGCACAAAATG+TGG - chr6.2:145046-145065 None:intergenic 35.0%
! ATCAGTAGTTGATATTGTGC+CGG - chr6.2:145486-145505 None:intergenic 35.0%
! TAATTTGCCAAAGGCTCAAA+AGG + chr6.2:144187-144206 MS.gene053100:intron 35.0%
! TCTCTAAGAATCTCAGCTAA+AGG - chr6.2:142071-142090 None:intergenic 35.0%
! TGATAAGTTCCGTGGATTTT+TGG - chr6.2:145276-145295 None:intergenic 35.0%
!! AATGAAGAAAGTACTGACCA+GGG - chr6.2:142173-142192 None:intergenic 35.0%
!! CAAACTGGTTTGTAAGTTGA+GGG + chr6.2:142971-142990 MS.gene053100:intron 35.0%
!! TAACTTTCTTTGTTGATGGC+AGG + chr6.2:145445-145464 MS.gene053100:intron 35.0%
!! TAATGAAGAAAGTACTGACC+AGG - chr6.2:142174-142193 None:intergenic 35.0%
!! TATGTTTTTGTAGGATGGTG+TGG + chr6.2:142553-142572 MS.gene053100:intron 35.0%
!! TGTAAAGTGGTGGATTTAGT+TGG - chr6.2:143026-143045 None:intergenic 35.0%
!!! CTTTGATTGGCTTATTTTCG+AGG + chr6.2:142306-142325 MS.gene053100:intron 35.0%
!!! TTTTTTTGCTATTGGCCTAG+TGG - chr6.2:144869-144888 None:intergenic 35.0%
AACATAGGTGATAAGTTCCG+TGG - chr6.2:145284-145303 None:intergenic 40.0%
AGTCCAATATGCAGGTAATG+TGG - chr6.2:143323-143342 None:intergenic 40.0%
ATGTTAAGCTTCTATGCTCC+AGG + chr6.2:142475-142494 MS.gene053100:CDS 40.0%
CAAGCTTCTGACATGATTAG+TGG - chr6.2:145168-145187 None:intergenic 40.0%
CAATTATGGCTCCCTTTGAT+TGG + chr6.2:142293-142312 MS.gene053100:intron 40.0%
CACTACACTACAACAACACA+AGG - chr6.2:144791-144810 None:intergenic 40.0%
CATTACTAGGACTTGAGTGT+CGG + chr6.2:144816-144835 MS.gene053100:intron 40.0%
CCTCAACTTACAAACCAGTT+TGG - chr6.2:142973-142992 None:intergenic 40.0%
CGAGGTTATGTAAAAGAGCT+TGG + chr6.2:142324-142343 MS.gene053100:intron 40.0%
CTGCCTTCTAACACTATTCA+AGG + chr6.2:144729-144748 MS.gene053100:intron 40.0%
GAACCACATTACCTGCATAT+TGG + chr6.2:143317-143336 MS.gene053100:intron 40.0%
GACTAAAATCAACCTGACTC+TGG - chr6.2:144306-144325 None:intergenic 40.0%
GGAGAGAACAACAACAATAG+AGG - chr6.2:143286-143305 None:intergenic 40.0%
GGCACAATATCAACTACTGA+TGG + chr6.2:145485-145504 MS.gene053100:CDS 40.0%
GTGTAGTGGTTACCATTACT+AGG + chr6.2:144803-144822 MS.gene053100:intron 40.0%
TGCTGAAACTGGATCACTAA+TGG - chr6.2:145513-145532 None:intergenic 40.0%
! CTTGTGTTGTTGTAGTGTAG+TGG + chr6.2:144789-144808 MS.gene053100:intron 40.0%
! TGGCCTTGAATAGTGTTAGA+AGG - chr6.2:144735-144754 None:intergenic 40.0%
!! ATGAAGAAAGTACTGACCAG+GGG - chr6.2:142172-142191 None:intergenic 40.0%
!! CCAAACTGGTTTGTAAGTTG+AGG + chr6.2:142970-142989 MS.gene053100:intron 40.0%
!! CTTGCATTGGCTCAACTTAT+TGG + chr6.2:144480-144499 MS.gene053100:intron 40.0%
!! GAGGTTTAACGAGAGTATCA+GGG - chr6.2:142109-142128 None:intergenic 40.0%
!! GTAGAGTACCAGTGATTTTG+CGG - chr6.2:144915-144934 None:intergenic 40.0%
AAGGCTCAAAAGGATCTAGG+TGG + chr6.2:144197-144216 MS.gene053100:intron 45.0%
AGAAGCGGAGGAATTAGGAT+CGG - chr6.2:142135-142154 None:intergenic 45.0%
AGCTAAAGGACGAGTAATGG+TGG - chr6.2:142057-142076 None:intergenic 45.0%
AGGCTCAAAAGGATCTAGGT+GGG + chr6.2:144198-144217 MS.gene053100:intron 45.0%
CCAAAGGCTCAAAAGGATCT+AGG + chr6.2:144194-144213 MS.gene053100:intron 45.0%
CGACACTCAAGTCCTAGTAA+TGG - chr6.2:144818-144837 None:intergenic 45.0%
CTCAGCTAAAGGACGAGTAA+TGG - chr6.2:142060-142079 None:intergenic 45.0%
CTTTACACCCAGCACAATGA+AGG + chr6.2:143039-143058 MS.gene053100:intron 45.0%
CTTTCTGTAAAGCATGTGCG+CGG + chr6.2:145546-145565 MS.gene053100:CDS 45.0%
GAAGAAGAAGCGGAGGAATT+AGG - chr6.2:142140-142159 None:intergenic 45.0%
GATGAAGGTGGAAGAAGAAG+CGG - chr6.2:142150-142169 None:intergenic 45.0%
GTATACCGTCTTACTGTACG+CGG + chr6.2:142622-142641 MS.gene053100:CDS 45.0%
TAGTGATCCAGTTTCAGCAG+CGG + chr6.2:145514-145533 MS.gene053100:CDS 45.0%
TCATTGTGCTGGGTGTAAAG+TGG - chr6.2:143039-143058 None:intergenic 45.0%
TCCAACACCTTCATTGTGCT+GGG - chr6.2:143049-143068 None:intergenic 45.0%
TGGAAATTCCGCCTTCATTG+CGG - chr6.2:143122-143141 None:intergenic 45.0%
! AGCAGTGATGGAAAGAAAGG+AGG + chr6.2:141954-141973 MS.gene053100:CDS 45.0%
! AGTGATGGAAAGAAAGGAGG+AGG + chr6.2:141957-141976 MS.gene053100:CDS 45.0%
! CCTAGATCCTTTTGAGCCTT+TGG - chr6.2:144197-144216 None:intergenic 45.0%
! TTCGGCTTGAGTAGTGTAGA+AGG - chr6.2:144342-144361 None:intergenic 45.0%
!! GGAGGTTTAACGAGAGTATC+AGG - chr6.2:142110-142129 None:intergenic 45.0%
ACCCAAATGCCACAGTTAGG+CGG + chr6.2:143085-143104 MS.gene053100:intron 50.0%
AGCAGCAGCAATAGCAGTGA+TGG + chr6.2:141942-141961 MS.gene053100:CDS 50.0%
CAGATCCGCGTACAGTAAGA+CGG - chr6.2:142630-142649 None:intergenic 50.0%
CTCCAACACCTTCATTGTGC+TGG - chr6.2:143050-143069 None:intergenic 50.0%
CTTACTGTACGCGGATCTGA+TGG + chr6.2:142631-142650 MS.gene053100:CDS 50.0%
GCAACCCAAATGCCACAGTT+AGG + chr6.2:143082-143101 MS.gene053100:intron 50.0%
GCAATTTGCAAACACGCACC+TGG - chr6.2:142496-142515 None:intergenic 50.0%
TGTAAAGCATGTGCGCGGTT+TGG + chr6.2:145551-145570 MS.gene053100:CDS 50.0%
TTGGCTCAACTCATTGCTGG+AGG + chr6.2:142794-142813 MS.gene053100:intron 50.0%
TTGTGCTGGGTGTAAAGTGG+TGG - chr6.2:143036-143055 None:intergenic 50.0%
! AAGAAAGGAGGAGGTGTAGG+AGG + chr6.2:141966-141985 MS.gene053100:CDS 50.0%
! ACCCAGCACAATGAAGGTGT+TGG + chr6.2:143045-143064 MS.gene053100:intron 50.0%
! GAGAACGAATGCAAAGGCAC+TGG + chr6.2:145354-145373 MS.gene053100:intron 50.0%
! GGAAAGAAAGGAGGAGGTGT+AGG + chr6.2:141963-141982 MS.gene053100:CDS 50.0%
! TTGCTATTGGCCTAGTGGCA+GGG - chr6.2:144864-144883 None:intergenic 50.0%
! TTTTCCTCCGCTGCTGAAAC+TGG - chr6.2:145524-145543 None:intergenic 50.0%
!! AAGTACTGACCAGGGGATGA+AGG - chr6.2:142165-142184 None:intergenic 50.0%
!! CAGCACAATGAAGGTGTTGG+AGG + chr6.2:143048-143067 MS.gene053100:intron 50.0%
!! GCATTGGCTCAACTCATTGC+TGG + chr6.2:142791-142810 MS.gene053100:intron 50.0%
!! TTTGCTATTGGCCTAGTGGC+AGG - chr6.2:144865-144884 None:intergenic 50.0%
AATGCCACAGTTAGGCGGCT+AGG + chr6.2:143090-143109 MS.gene053100:intron 55.0%
AGCCGCCTAACTGTGGCATT+TGG - chr6.2:143090-143109 None:intergenic 55.0%
AGCGGAGGAATTAGGATCGG+TGG - chr6.2:142132-142151 None:intergenic 55.0%
ATGCCACAGTTAGGCGGCTA+GGG + chr6.2:143091-143110 MS.gene053100:intron 55.0%
CAGGCCTATCGTGAGTCAAC+CGG + chr6.2:145464-145483 MS.gene053100:intron 55.0%
CTTCTTCCACCTTCATCCCC+TGG + chr6.2:142153-142172 MS.gene053100:CDS 55.0%
GAACGTGGCCGCAAAATCAC+TGG + chr6.2:144904-144923 MS.gene053100:intron 55.0%
GAAGGTGGAAGAAGAAGCGG+AGG - chr6.2:142147-142166 None:intergenic 55.0%
GAGGAATTAGGATCGGTGGG+AGG - chr6.2:142128-142147 None:intergenic 55.0%
GCCGCCTAACTGTGGCATTT+GGG - chr6.2:143089-143108 None:intergenic 55.0%
GCGGAGGAATTAGGATCGGT+GGG - chr6.2:142131-142150 None:intergenic 55.0%
TACTGACCAGGGGATGAAGG+TGG - chr6.2:142162-142181 None:intergenic 55.0%
TGATCCAGTTTCAGCAGCGG+AGG + chr6.2:145517-145536 MS.gene053100:CDS 55.0%
TGTACGCGGATCTGATGGAG+AGG + chr6.2:142636-142655 MS.gene053100:CDS 55.0%
TTGGCTCAACTTGTCGCTGG+AGG + chr6.2:143376-143395 MS.gene053100:intron 55.0%
! GGTGTTGGAGGCTGCAATGA+AGG + chr6.2:143060-143079 MS.gene053100:intron 55.0%
! TGCTATTGGCCTAGTGGCAG+GGG - chr6.2:144863-144882 None:intergenic 55.0%
!! GCATTGGCTCAACTTGTCGC+TGG + chr6.2:143373-143392 MS.gene053100:intron 55.0%
!! TGTGCCGGTTGACTCACGAT+AGG - chr6.2:145471-145490 None:intergenic 55.0%
ACAGTTAGGCGGCTAGGGAC+GGG + chr6.2:143096-143115 MS.gene053100:intron 60.0%
CACAGTTAGGCGGCTAGGGA+CGG + chr6.2:143095-143114 MS.gene053100:intron 60.0%
! GCTATTGGCCTAGTGGCAGG+GGG - chr6.2:144862-144881 None:intergenic 60.0%
!! GCATGTGCGCGGTTTGGTCT+TGG + chr6.2:145557-145576 MS.gene053100:CDS 60.0%
CGTCCCTAGCCGCCTAACTG+TGG - chr6.2:143097-143116 None:intergenic 65.0%
CTAGGGACGGGCCGCAATGA+AGG + chr6.2:143108-143127 MS.gene053100:intron 65.0%
TTGGCCTAGTGGCAGGGGGT+GGG - chr6.2:144858-144877 None:intergenic 65.0%
! ATTGGCCTAGTGGCAGGGGG+TGG - chr6.2:144859-144878 None:intergenic 65.0%
GGGACGGGCCGCAATGAAGG+CGG + chr6.2:143111-143130 MS.gene053100:intron 70.0%
TGGCCTAGTGGCAGGGGGTG+GGG - chr6.2:144857-144876 None:intergenic 70.0%
GCACCCCACCCCCTGCCACT+AGG + chr6.2:144851-144870 MS.gene053100:intron 75.0%
!! GGCAGGGGGTGGGGTGCTGT+GGG - chr6.2:144848-144867 None:intergenic 75.0%
!! TGGCAGGGGGTGGGGTGCTG+TGG - chr6.2:144849-144868 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 141798 145619 141798 ID=MS.gene053100
chr6.2 mRNA 141798 145619 141798 ID=MS.gene053100.t1;Parent=MS.gene053100
chr6.2 exon 141798 142174 141798 ID=MS.gene053100.t1.exon1;Parent=MS.gene053100.t1
chr6.2 CDS 141798 142174 141798 ID=cds.MS.gene053100.t1;Parent=MS.gene053100.t1
chr6.2 exon 142459 142496 142459 ID=MS.gene053100.t1.exon2;Parent=MS.gene053100.t1
chr6.2 CDS 142459 142496 142459 ID=cds.MS.gene053100.t1;Parent=MS.gene053100.t1
chr6.2 exon 142566 142657 142566 ID=MS.gene053100.t1.exon3;Parent=MS.gene053100.t1
chr6.2 CDS 142566 142657 142566 ID=cds.MS.gene053100.t1;Parent=MS.gene053100.t1
chr6.2 exon 145467 145619 145467 ID=MS.gene053100.t1.exon4;Parent=MS.gene053100.t1
chr6.2 CDS 145467 145619 145467 ID=cds.MS.gene053100.t1;Parent=MS.gene053100.t1
Gene Sequence

>MS.gene053100

ATGGCATTTTCAGTTTGCAAAACGATTCCATTCACTTCTTCTTCTTCATCTTTCTACAAAGAAATCTCAATTGAACCATCATCAATATGGTTTACAAGAAAGACGAGAAGTATTGGTGTCATCAAATGTTCTATTGAACGCAGTAGCAGCAGCAATAGCAGTGATGGAAAGAAAGGAGGAGGTGTAGGAGGAGTAGTATCGAATTCAAATTATGTTGTACCTTTGGATGATTCATTTTCATTCTCAAATTCATCTTCCACCATTACTCGTCCTTTAGCTGAGATTCTTAGAGACCTTAATAAGCGAATCCCTGATACTCTCGTTAAACCTCCCACCGATCCTAATTCCTCCGCTTCTTCTTCCACCTTCATCCCCTGGTCAGTACTTTCTTCATTACTTTCTATTATTTGTATTGTCTCTGTAAGCACCGACATTTCTGATCGAAGACTATGCTTGATATCTCACAACTAAACAACATATTCATGTGATTAAGTTCAATTATGGCTCCCTTTGATTGGCTTATTTTCGAGGTTATGTAAAAGAGCTTGGTTTGGTTATGCAAATAAATACGCTTTTATATATTGTTTATAGCTTGTTTATTGTCATTATTATTTTTTATGATCTAATTTTACTTTCAATTTATCTGCTTGTTCTTTGATAGGTATCATGCTAATAGGATGTTAAGCTTCTATGCTCCAGGTGCGTGTTTGCAAATTGCAAAGTGTTTTTGAATTTAGATAGTTTTGAATTTGGAATATGTTTTTGTAGGATGGTGTGGAGAGATACGTGATGTTATTTTCTCTGATAATGGAACTGTAACTGTTGTATACCGTCTTACTGTACGCGGATCTGATGGAGAGGTACCTACCCTCATCTAATCTATCATGTTTATTCATTCATACTCTATTGTTGTTGTTCTGTCCTTCTTGCTTCTAAAACACATTATGTGGATATTGAACTTAGAAGAATATTGTTATCATGTTAAATAAACTCGCATTGGCTCAACTCATTGCTGGAGGATAATGTTTTGAACAACCCATTATTATGATTTAAATCTGATGTTCTTATTAGATTCAATATGATCTAGGTGGACATGTTATGCTTCTGAGTGATATTGTATCAGCTTTTATGCCATTATTCTTAGAATTTTGGTTGAGCATAACTCAACCTTACCAAACTGGTTTGTAAGTTGAGGGCTGCCCAAACTTTATAAACATTATATAGGCCAACTAAATCCACCACTTTACACCCAGCACAATGAAGGTGTTGGAGGCTGCAATGAAGGCAACCCAAATGCCACAGTTAGGCGGCTAGGGACGGGCCGCAATGAAGGCGGAATTTCCAACAATTATATATGCTAGTAATCTCATCAAAAGTTTATTATGTCTGTACTTGTACCCAAAATCTAAATTCAGAGTCAGATTGATTAAGTTTGATAGTGCACTCACCCTAACTTTCTACTATTCCATTCATTCATATTTGCTACCTCTATTGTTGTTGTTCTCTCCTTTTTTCTTCTGAACCACATTACCTGCATATTGGACTTTGAATATCGTTATTATGTTAAATATACTTGCATTGGCTCAACTTGTCGCTGGAGGATATGTAATCTTATGAACAACCCATCGTTCTGATTTGAAATTTCTGTTCTCGTTAGATTTGATATGATCTAGGCGGGTATATTATGCTTCTGAGTGATATTGTATCGGCTTCTATGCCATTATATATGCTAGCAATCTTGTCAAAAGTTTCTTATGTCTGTTATTGGACCCTACATTTAAATTCGGAGTCAGATTTAAGTCTGATGATGCACTACCATCTACACTATTTAATCCCAAAACCTCAAATTATCAACTATGTAGATGTTGGATTCTAAGTCAATATTGTAATCTATATATCAACTCTTTCAAAGCATGTGTGAAAGAATGTGGCCCGTGTGAAATCGCTAATAATCTACTAATTGATATTATAAAATGAACTATGTGAAATCTTCCTTCTAACTTCTAATGCAATCATTCATATACTTGCCACCTCTATTCTTCTGTTTTTTTCCTTCTAAAATACATTCTGTGCAAATTGGACTTTGAACATTATTATGATTTTAAACTTGCATTGCACTTTCTGAACTTATTGGAATTGGAGGATATGTAATCTTCTTAACGAACCGTTATTATGATTGAGAAATGATATTTGTACAACTATTTTGTGACAACTTTTGGGACAGCCTTATTTTTCTCTCTTCTTATTGGTCCAAAACAATAGATAGAAATAGGAAGAGAGAGTAAGAACACAATGTGAGTGTGAGAGAGAAAGTTGTCACAAAATATTTGTACAAATACCATTTCTCATTATGATTTAAAGCTTTTGTTCTTACTACGTGAATAGAATATAATTTGCCAAAGGCTCAAAAGGATCTAGGTGGGCTGTCATGCTTCTCATATTGTATGACCTTTTATATGTGCCACTAATCTTATCAAAAACATTTTCTGTGCCCTGAATTTAAATCCAGAGTCAGGTTGATTTTAGTCTAATAGTGCATTACCTTCTACACTACTCAAGCCGAAAAATCACAGAACTTTTCACCTATGTTTGATTCTAAGTGGATATTTAATTTATATATCAACTCTATTACTTGTTGAAATTACAAAATGTACTTTGAATATTGTTATCATGTTAAATAAACTTGCATTGGCTCAACTTATTGGTAGATATGTAATCTTCTAAACAACCCCTCATTTTGATTTAAAGCTTCTATTATTATTAGATTCAACATGATCTAGACGTGCATGTTATGTTTTTGACTGATATTGTATCAACTTTTATGCCATTTATATAATGCCACTAACCTTATCAAAAGTTTGTCATGTGATGTCTATACTTGCAGTACCCTTAATCTAAAATCAGAGTCAGATTATTAAGTCTGACAGTGCACTGCCTTCTAACACTATTCAAGGCCAAAAATCTCTTAAATTATCAACAATTTTTTAGTCCCTTGTGTTGTTGTAGTGTAGTGGTTACCATTACTAGGACTTGAGTGTCGGTTCGAACCCACAGCACCCCACCCCCTGCCACTAGGCCAATAGCAAAAAAATAATAAAATGAAAATGAACGTGGCCGCAAAATCACTGGTACTCTACTCCTTGAAATTATAGATGAATTAAAGGGCAGCGTCCTATTTTACTTCTAATGCATTCATTCATATTTGCCACCTACCTCTATTCTTTTTCTGTCCTTTTTCTCTCTAACCACATTTTGTGCAAATTAGCCTTTGAACATTATTACATTGAATTGAATATAATTTGGAAAATGTCCAATATGATTATGGTAGGCTGTCATGCTTCTTATGTTGTATCACCTTTTATATATGCCACTAATCATGTCAGAAGCTTGTCATGTCTATATTTGCACCATAAATCTAAATCCAGAGTAATATTGATTTAAGTTTAATAGCGCATTAGCTTTTACACTATTCAAGCCAAAAATCCACGGAACTTATCACCTATGTTTGATTCTAAGTGCATACTTATTATACATATGAGCTCTTTAAAACATATCTGAGAACGAATGCAAAGGCACTGGTACTATACTAGTTGAAATTACAAAATGAACTATAGAACAACTATCTAATTCTAAATGTCCAAAATGACTAACTTTCTTTGTTGATGGCAGGCCTATCGTGAGTCAACCGGCACAATATCAACTACTGATGGCTCCATTAGTGATCCAGTTTCAGCAGCGGAGGAAAATGCTTTCTGTAAAGCATGTGCGCGGTTTGGTCTTGGTTTGTATTTGTATCATGATGATCAAACAACCAGTATGTAA
Protein sequence

>MS.gene053100.t1

MAFSVCKTIPFTSSSSSFYKEISIEPSSIWFTRKTRSIGVIKCSIERSSSSNSSDGKKGGGVGGVVSNSNYVVPLDDSFSFSNSSSTITRPLAEILRDLNKRIPDTLVKPPTDPNSSASSSTFIPWYHANRMLSFYAPGWCGEIRDVIFSDNGTVTVVYRLTVRGSDGEAYRESTGTISTTDGSISDPVSAAEENAFCKACARFGLGLYLYHDDQTTSM