AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053973

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053973.t1 MTR_5g057990 84.370 627 23 3 5 561 294 915 0.0 1056
MS.gene053973.t1 MTR_1g110980 69.856 627 109 5 5 561 297 913 0.0 896
MS.gene053973.t1 MTR_1g111000 65.835 641 133 4 5 564 294 929 0.0 865
MS.gene053973.t1 MTR_3g096870 32.409 469 264 14 21 452 356 808 6.04e-67 238
MS.gene053973.t1 MTR_5g026610 30.335 478 273 12 21 462 204 657 2.21e-56 207
MS.gene053973.t1 MTR_5g026610 30.544 478 272 13 21 462 204 657 2.42e-56 208
MS.gene053973.t1 MTR_5g026610 30.544 478 272 13 21 462 267 720 2.86e-56 208
MS.gene053973.t1 MTR_5g026610 30.335 478 273 12 21 462 267 720 2.96e-56 207
MS.gene053973.t1 MTR_5g026610 30.544 478 272 13 21 462 307 760 5.36e-56 207
MS.gene053973.t1 MTR_5g026610 29.730 444 252 13 55 462 2 421 1.61e-45 174
MS.gene053973.t1 MTR_4g020770 77.273 88 20 0 578 665 73 160 4.89e-36 136
MS.gene053973.t1 MTR_5g048690 79.452 73 15 0 593 665 92 164 6.93e-30 118
MS.gene053973.t1 MTR_6g014900 64.706 51 18 0 285 335 82 132 3.53e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053973.t1 AT1G68560 65.127 628 140 6 5 561 283 902 0.0 829
MS.gene053973.t1 AT3G45940 62.222 585 171 7 5 561 280 842 0.0 734
MS.gene053973.t1 AT5G11720 46.542 535 230 5 5 491 302 828 9.40e-166 500
MS.gene053973.t1 AT5G63840 33.058 484 263 13 20 462 364 827 1.10e-69 245
MS.gene053973.t1 AT5G63840 33.058 484 263 13 20 462 391 854 1.26e-69 246
MS.gene053973.t1 AT3G23640 31.250 480 262 14 21 464 201 648 6.90e-63 227
MS.gene053973.t1 AT3G23640 31.250 480 262 14 21 464 201 648 6.90e-63 227
MS.gene053973.t1 AT3G23640 32.033 487 249 16 21 464 270 717 9.09e-63 227

Find 196 sgRNAs with CRISPR-Local

Find 285 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACATGTGCTTCGTAGTTTA+AGG 0.126958 5.2:+42614224 None:intergenic
CCGTTTCCAAGCTTCATTTC+TGG 0.183467 5.2:+42613799 None:intergenic
AAGGAACGTGGGAGAATTTA+AGG 0.205529 5.2:-42614500 MS.gene053973:CDS
TTTGCATGATCTCTTGAAAA+TGG 0.216652 5.2:+42614348 None:intergenic
GTCATTATTGACCCTGGAAT+TGG 0.270891 5.2:-42615101 MS.gene053973:CDS
TCCAAGTTTGGATAAGTTAA+AGG 0.271557 5.2:-42612353 None:intergenic
ATGTTGAATTTCGGTATATT+TGG 0.272681 5.2:-42614462 MS.gene053973:CDS
TTCAGTCGTTCGGTGTTTAA+AGG 0.283024 5.2:-42612893 MS.gene053973:intron
TTAGATGGAAAGCCACTAAT+TGG 0.284571 5.2:-42613613 MS.gene053973:CDS
CTAGAAATGCTTTAGGTATA+AGG 0.284843 5.2:-42614272 MS.gene053973:CDS
TACTGTTGCTAATGGTTCTA+AGG 0.291460 5.2:-42612665 MS.gene053973:CDS
GGTGTGAATGTTACTACTTC+TGG 0.291490 5.2:-42613583 MS.gene053973:CDS
ACCCACCAAGATACCGTCTT+TGG 0.312992 5.2:+42614953 None:intergenic
ATCCCACAGCCTTGATCATT+TGG 0.313966 5.2:-42615688 MS.gene053973:CDS
CACGCTCGTTGTTACATTCC+CGG 0.323995 5.2:-42613878 MS.gene053973:CDS
TTCTATTTCTGTGTTGGAAT+TGG 0.325907 5.2:-42613668 MS.gene053973:CDS
TTAGGACGAGGGTAGTTCTT+TGG 0.326153 5.2:+42615173 None:intergenic
CCTTACAAGATAAACGCTTC+TGG 0.335424 5.2:-42614738 MS.gene053973:CDS
AACAAGGAGGTGTGAAAGTT+TGG 0.339571 5.2:-42613741 MS.gene053973:CDS
TCATTATTGACCCTGGAATT+GGG 0.345196 5.2:-42615100 MS.gene053973:CDS
CCAGAAGCGTTTATCTTGTA+AGG 0.347793 5.2:+42614738 None:intergenic
GTGGCTTCACTTAAGTTAGA+TGG 0.348861 5.2:-42613628 MS.gene053973:CDS
GGTTCTAAGGCACAAGTTAC+TGG 0.349182 5.2:-42612652 MS.gene053973:CDS
AGATATCATTCATCGTCGTT+TGG 0.354048 5.2:-42612380 MS.gene053973:CDS
GGTGCTTCTGATCATGTTGT+TGG 0.359051 5.2:-42612739 MS.gene053973:CDS
AGGATCGTCCCATCTTGTTT+TGG 0.363147 5.2:+42614761 None:intergenic
CGGTCCACTTATGTTGGTTC+GGG 0.364125 5.2:-42614555 MS.gene053973:CDS
GTGGACCGTGACAAAATAAA+AGG 0.365865 5.2:+42614570 None:intergenic
GTCTTTGGATTGAGAAAATC+AGG 0.367465 5.2:+42614968 None:intergenic
TATGATGCTCATAGTATCTA+TGG 0.368905 5.2:-42614648 MS.gene053973:CDS
ACGGTCCACTTATGTTGGTT+CGG 0.369739 5.2:-42614556 MS.gene053973:CDS
GCAACTCACAAGGCTCTTCT+TGG 0.370407 5.2:-42614609 MS.gene053973:CDS
GTAATGTTGTGAGGTATCTT+AGG 0.374270 5.2:+42612685 None:intergenic
CTGGAGTTAACCCCAATTCC+AGG 0.374518 5.2:+42615089 None:intergenic
CTGAAGAACTATGCAATAGA+TGG 0.376212 5.2:-42614392 MS.gene053973:CDS
ATATCCGCAATGTTGAATTT+CGG 0.382214 5.2:-42614471 MS.gene053973:CDS
CATAGTTCTTCAGTTGGTTG+TGG 0.383745 5.2:+42614402 None:intergenic
GATATCATTCATCGTCGTTT+GGG 0.384444 5.2:-42612379 MS.gene053973:CDS
AAGGCACAAGTTACTGGTAT+TGG 0.384733 5.2:-42612646 MS.gene053973:CDS
CCTTTGGTATACACCATAAC+TGG 0.387237 5.2:+42615070 None:intergenic
GATATCCCCATCTGCATTGA+TGG 0.387246 5.2:+42615312 None:intergenic
ACCTTTAACTTATCCAAACT+TGG 0.387729 5.2:+42612352 None:intergenic
AAACGACGATGAATGATATC+TGG 0.391226 5.2:+42612382 None:intergenic
CACAACATTACTGTTGCTAA+TGG 0.393352 5.2:-42612673 MS.gene053973:CDS
TGATCATTTGGACCGGCTGC+GGG 0.393851 5.2:-42615676 MS.gene053973:CDS
TACTTCTGGACATGTGCATT+TGG 0.395816 5.2:-42613569 MS.gene053973:CDS
TCAGTCGTTCGGTGTTTAAA+GGG 0.402758 5.2:-42612892 MS.gene053973:intron
ACTAGTGCAACCCACTATAA+TGG 0.403296 5.2:-42614681 MS.gene053973:CDS
ATCAATCCACCTTGCTGCAT+TGG 0.404465 5.2:+42613928 None:intergenic
TAGTTTAAGGTGTAAAGATA+TGG 0.405653 5.2:+42614237 None:intergenic
AAGGCTCTTCTTGGACTTGA+AGG 0.410685 5.2:-42614600 MS.gene053973:CDS
ACACCTGACAAGCCAATTAG+TGG 0.411800 5.2:+42613601 None:intergenic
TCGTTTGGGTCATCCAAGTT+TGG 0.413409 5.2:-42612365 MS.gene053973:CDS
TTCAAGAAGTTTAGAAGCTT+AGG 0.417930 5.2:+42615155 None:intergenic
CAATCTGCTAGAAATGCTTT+AGG 0.418336 5.2:-42614279 MS.gene053973:CDS
TTGATCATTTGGACCGGCTG+CGG 0.418694 5.2:-42615677 MS.gene053973:CDS
TCAATCCAAAGACGGTATCT+TGG 0.419549 5.2:-42614958 MS.gene053973:CDS
ATGATCTAGTAGAGTGAGAT+AGG 0.430263 5.2:+42612776 None:intergenic
TCTCATCCATCCACCATTTG+TGG 0.430398 5.2:-42612418 MS.gene053973:CDS
TTTGTTTCGTGTTGTTTGAT+GGG 0.432443 5.2:-42612856 MS.gene053973:CDS
CGAAGTACGGGAAAGACAAT+TGG 0.432605 5.2:-42612478 MS.gene053973:CDS
TTCTTGGACTTGAAGGCAAA+AGG 0.442261 5.2:-42614593 MS.gene053973:CDS
AAGCCACTAATTGGCTTGTC+AGG 0.443897 5.2:-42613604 MS.gene053973:CDS
AAGAAAAGTGGTCTCGCAAT+TGG 0.443977 5.2:+42614192 None:intergenic
AATGTAACAACGAGCGTGAA+TGG 0.445267 5.2:+42613883 None:intergenic
TATCTCACTCTACTAGATCA+TGG 0.447575 5.2:-42612774 MS.gene053973:CDS
TCTCCTTCTGATGCGCCAGC+CGG 0.447588 5.2:+42613859 None:intergenic
GCAAGGTGGATTGATCTCTA+AGG 0.447708 5.2:-42613920 MS.gene053973:CDS
ATAGTTCTTCAGTTGGTTGT+GGG 0.451320 5.2:+42614403 None:intergenic
ATTGATAAAGCTCTTGTCTC+GGG 0.451344 5.2:+42614316 None:intergenic
AAATTCCACTAGATGTGATC+TGG 0.454411 5.2:-42615241 MS.gene053973:CDS
ATTGGGGTTAACTCCAGTTA+TGG 0.456204 5.2:-42615083 MS.gene053973:intron
AACTACGAAGCACATGTTAG+TGG 0.458451 5.2:-42614219 MS.gene053973:CDS
CTCCTTCTGATGCGCCAGCC+GGG 0.463891 5.2:+42613860 None:intergenic
TTGCATGATCTCTTGAAAAT+GGG 0.465416 5.2:+42614349 None:intergenic
TATTGATTCTATTTCTGTGT+TGG 0.472331 5.2:-42613674 MS.gene053973:CDS
CTCGTTGTTACATTCCCGGC+TGG 0.472667 5.2:-42613874 MS.gene053973:CDS
ACTCCAAGACACCATTATAG+TGG 0.472781 5.2:+42614670 None:intergenic
GTTGGTACAGTTTACTCGAT+TGG 0.473073 5.2:-42614044 MS.gene053973:CDS
GAGACAAGAGCTTTATCAAT+GGG 0.473675 5.2:-42614313 MS.gene053973:CDS
CTGGAACGACGATGATCATA+TGG 0.474710 5.2:-42615222 MS.gene053973:CDS
CGTTGTTTCCTCCCGGTAGT+TGG 0.476352 5.2:-42614062 MS.gene053973:CDS
CGGTATATTTGGTGTTCCAA+TGG 0.480390 5.2:-42614451 MS.gene053973:CDS
TACGAATAACACATAATTCA+AGG 0.482159 5.2:+42612597 None:intergenic
ACTCACACCATCACATCAAC+AGG 0.482302 5.2:-42614021 MS.gene053973:CDS
GGATACCCTGGTGGACTCAT+TGG 0.482748 5.2:+42615707 None:intergenic
TATATTTGGTGTTCCAATGG+TGG 0.488042 5.2:-42614448 MS.gene053973:CDS
GTGTTTAAAGGGAACCGTCC+TGG 0.488801 5.2:-42612881 MS.gene053973:CDS
CTATTGCATAGTTCTTCAGT+TGG 0.495820 5.2:+42614396 None:intergenic
GGTAAATTTGCTTTAGACAA+AGG 0.501257 5.2:-42613703 MS.gene053973:CDS
GGCTCATTTCAACAATTCTA+CGG 0.506523 5.2:-42612803 MS.gene053973:CDS
AGATGCAGAAACACCACAAA+TGG 0.507268 5.2:+42612405 None:intergenic
ATATTTGGTGTTCCAATGGT+GGG 0.513486 5.2:-42614447 MS.gene053973:CDS
CATTGTACTGGTGACAATCA+AGG 0.514193 5.2:-42614519 MS.gene053973:CDS
GAACTTCCAGAAATGAAGCT+TGG 0.516701 5.2:-42613805 MS.gene053973:CDS
AAATATGCTGCACATTGTAC+TGG 0.519641 5.2:-42614531 MS.gene053973:CDS
CGAGACAAGAGCTTTATCAA+TGG 0.520278 5.2:-42614314 MS.gene053973:CDS
CCAGAAATGAAGCTTGGAAA+CGG 0.521555 5.2:-42613799 MS.gene053973:CDS
CAGGATTCCATCAATGCAGA+TGG 0.521721 5.2:-42615319 MS.gene053973:CDS
ACCGGTGTTAGTCCCGCAGC+CGG 0.522106 5.2:+42615664 None:intergenic
CAATTGTCTTTCCCGTACTT+CGG 0.523554 5.2:+42612479 None:intergenic
ATCCAAAGACGGTATCTTGG+TGG 0.524403 5.2:-42614955 MS.gene053973:CDS
ATTATAGTGGGTTGCACTAG+TGG 0.525372 5.2:+42614683 None:intergenic
CTTATACAAGACCGAAGTAC+GGG 0.525723 5.2:-42612490 MS.gene053973:intron
TTTGCCCGAACCAACATAAG+TGG 0.526839 5.2:+42614551 None:intergenic
TGACAAATTAGAGATAAGAG+AGG 0.528013 5.2:+42612711 None:intergenic
AGTTTCCTCTTCAGCCACAT+TGG 0.534230 5.2:-42612824 MS.gene053973:CDS
TTCCCGGCTGGCGCATCAGA+AGG 0.535939 5.2:-42613862 MS.gene053973:CDS
AGTGCTGACGTCGTAACATT+TGG 0.536417 5.2:+42614152 None:intergenic
CATTATTGACCCTGGAATTG+GGG 0.536616 5.2:-42615099 MS.gene053973:CDS
TACATTGTCATTATTGACCC+TGG 0.538902 5.2:-42615107 MS.gene053973:CDS
ATCTCACTCTACTAGATCAT+GGG 0.540469 5.2:-42612773 MS.gene053973:CDS
AAGAGCCTTGTGAGTTGCGA+CGG 0.543637 5.2:+42614614 None:intergenic
CATTGATAAAGCTCTTGTCT+CGG 0.548717 5.2:+42614315 None:intergenic
ATCAGTTGTGGAAGATGTTG+TGG 0.549097 5.2:-42615282 MS.gene053973:CDS
CCTTATACAAGACCGAAGTA+CGG 0.550555 5.2:-42612491 MS.gene053973:intron
GTGAAAGCGTTGTTTCCTCC+CGG 0.551187 5.2:-42614069 MS.gene053973:CDS
GAGTAAACTGTACCAACTAC+CGG 0.552239 5.2:+42614050 None:intergenic
TTGGAATTGGATGGAAGTGT+TGG 0.552577 5.2:-42613655 MS.gene053973:CDS
GGTCCAAATGATCAAGGCTG+TGG 0.552883 5.2:+42615685 None:intergenic
AGAACATCACCAAAACAAGA+TGG 0.554704 5.2:-42614770 MS.gene053973:CDS
ATTTCTGTGTTGGAATTGGA+TGG 0.556400 5.2:-42613664 MS.gene053973:CDS
ATATCTCCGGTGCTCGAACA+AGG 0.557999 5.2:-42614102 MS.gene053973:CDS
CGTAACATTTGGTGTAAGAC+GGG 0.558019 5.2:+42614163 None:intergenic
GACACTCCAATGAGTCCACC+AGG 0.562115 5.2:-42615713 None:intergenic
AAGCAGTCTCATGATATCTC+CGG 0.562483 5.2:-42614115 MS.gene053973:CDS
TTGTCACGGTCCACTTATGT+TGG 0.565518 5.2:-42614561 MS.gene053973:CDS
TCTGGAATACAAGCTCCAAT+CGG 0.565528 5.2:-42614720 MS.gene053973:CDS
AGTTCTGCCTGTTGATGTGA+TGG 0.569266 5.2:+42614014 None:intergenic
GCAGCCGGTCCAAATGATCA+AGG 0.569449 5.2:+42615679 None:intergenic
TGCAATAGATGGATTGAAGT+TGG 0.569941 5.2:-42614381 MS.gene053973:CDS
CATATGTCAGAACCCACCAT+TGG 0.570826 5.2:+42614435 None:intergenic
ATGAGCCAATGTGGCTGAAG+AGG 0.571416 5.2:+42612819 None:intergenic
ACAGCCTTGATCATTTGGAC+CGG 0.571443 5.2:-42615683 MS.gene053973:CDS
TGAACATTGACCACATGAAG+AGG 0.574774 5.2:+42613973 None:intergenic
AAGTTTAGAAGCTTAGGACG+AGG 0.575518 5.2:+42615161 None:intergenic
TTGTTTCGTGTTGTTTGATG+GGG 0.575989 5.2:-42612855 MS.gene053973:CDS
AACCCACTATAATGGTGTCT+TGG 0.578349 5.2:-42614673 MS.gene053973:CDS
TGCAGAAACACCACAAATGG+TGG 0.579049 5.2:+42612408 None:intergenic
AGGATTCCATCAATGCAGAT+GGG 0.583052 5.2:-42615318 MS.gene053973:CDS
AAATGGTGGATGGATGAGAA+TGG 0.585230 5.2:+42612422 None:intergenic
ATCAAGGCTGTGGGATACCC+TGG 0.586788 5.2:+42615695 None:intergenic
GACAAATTAGAGATAAGAGA+GGG 0.591372 5.2:+42612712 None:intergenic
ATATCACAATCTATCAGTTG+TGG 0.592012 5.2:-42615294 MS.gene053973:CDS
AGTTTAGAAGCTTAGGACGA+GGG 0.592715 5.2:+42615162 None:intergenic
TTCAAGGATAGAGATGGCAG+AGG 0.595494 5.2:+42612613 None:intergenic
AATGTGGCTGAAGAGGAAAC+TGG 0.595789 5.2:+42612826 None:intergenic
GCATCAGAAGGAGATGCTAA+AGG 0.595910 5.2:-42613850 MS.gene053973:CDS
TGGAGTTAACCCCAATTCCA+GGG 0.596103 5.2:+42615090 None:intergenic
CTCCAAGACACCATTATAGT+GGG 0.597207 5.2:+42614671 None:intergenic
GACGGGAAAGTGAAGAAAAG+TGG 0.599323 5.2:+42614180 None:intergenic
TTGAACAAGATACACAGCAT+TGG 0.600111 5.2:-42615137 MS.gene053973:CDS
CATTTGGAAGGTGAAGGAAA+TGG 0.600808 5.2:-42613553 MS.gene053973:CDS
GCAATCCGTCGCAACTCACA+AGG 0.601545 5.2:-42614619 MS.gene053973:CDS
ACCGGCTGCGGGACTAACAC+CGG 0.601812 5.2:-42615665 MS.gene053973:intron
TCGTTCCAGATCACATCTAG+TGG 0.603387 5.2:+42615236 None:intergenic
GAACATCACCAAAACAAGAT+GGG 0.603407 5.2:-42614769 MS.gene053973:CDS
TCCAAAGACGGTATCTTGGT+GGG 0.603568 5.2:-42614954 MS.gene053973:CDS
ACTGTGATGGTAACTTTGAG+AGG 0.604296 5.2:-42613520 MS.gene053973:CDS
GGATGGAAGTGTTGGAGCAG+TGG 0.604723 5.2:-42613647 MS.gene053973:CDS
TCTGGACATGTGCATTTGGA+AGG 0.605601 5.2:-42613565 MS.gene053973:CDS
TCGTAACATTTGGTGTAAGA+CGG 0.606150 5.2:+42614162 None:intergenic
ATGTTGTGAGGTATCTTAGG+AGG 0.609436 5.2:+42612688 None:intergenic
TGTTGTGGATAGCTACAAGA+AGG 0.611280 5.2:-42615267 MS.gene053973:CDS
CTATATGCTAGTGTCAAACA+AGG 0.612842 5.2:-42613757 MS.gene053973:CDS
GTCCAAATGATCAAGGCTGT+GGG 0.613002 5.2:+42615686 None:intergenic
GAAACACCACAAATGGTGGA+TGG 0.613285 5.2:+42612412 None:intergenic
ACTTGATGCACCTCTTCATG+TGG 0.619411 5.2:-42613983 MS.gene053973:CDS
ACATAATTCAAGGATAGAGA+TGG 0.622541 5.2:+42612607 None:intergenic
TTGATAAAGCTCTTGTCTCG+GGG 0.623923 5.2:+42614317 None:intergenic
GAAGCGTTTATCTTGTAAGG+AGG 0.630030 5.2:+42614741 None:intergenic
GTCAGCACTCAGTTTCTACT+CGG 0.632903 5.2:-42614138 MS.gene053973:CDS
CTGGTGACAATCAAGGAACG+TGG 0.634056 5.2:-42614512 MS.gene053973:CDS
AGTTTGGTCAGAAGTTCAAG+AGG 0.635859 5.2:-42613725 MS.gene053973:CDS
AGTAAACTGTACCAACTACC+GGG 0.640442 5.2:+42614051 None:intergenic
AAGGCTGTGGGATACCCTGG+TGG 0.645429 5.2:+42615698 None:intergenic
AGAGGGTTGAGCATTCCTGT+TGG 0.649091 5.2:-42613502 MS.gene053973:intron
TGGCTGAAGAGGAAACTGGT+TGG 0.652223 5.2:+42612830 None:intergenic
GTTTGGTCAGAAGTTCAAGA+GGG 0.655783 5.2:-42613724 MS.gene053973:CDS
ACAATTCTTCCAATGCAGCA+AGG 0.659787 5.2:-42613937 MS.gene053973:CDS
ATGGTGGGTTCTGACATATG+TGG 0.665102 5.2:-42614432 MS.gene053973:CDS
ATTCTTCCAATGCAGCAAGG+TGG 0.666214 5.2:-42613934 MS.gene053973:CDS
AACGACGATGATCATATGGA+CGG 0.669594 5.2:-42615218 MS.gene053973:CDS
GGAACAAGATCTCAATCACA+AGG 0.670205 5.2:-42612457 MS.gene053973:CDS
CTGTGATGGTAACTTTGAGA+GGG 0.681039 5.2:-42613519 MS.gene053973:CDS
TGGTGAAAGTAAGACTGTGA+TGG 0.681480 5.2:-42613533 MS.gene053973:CDS
GTACGAGTTCATGAAAGCGA+CGG 0.689303 5.2:+42614921 None:intergenic
GGATTCCATCAATGCAGATG+GGG 0.692287 5.2:-42615317 MS.gene053973:CDS
TGGTGACAATCAAGGAACGT+GGG 0.696940 5.2:-42614511 MS.gene053973:CDS
TATACCGAAATTCAACATTG+CGG 0.698974 5.2:+42614467 None:intergenic
TTAGCAACAGTAATGTTGTG+AGG 0.703191 5.2:+42612676 None:intergenic
ATTGTTGAAATGAGCCAATG+TGG 0.710467 5.2:+42612810 None:intergenic
CATGTGCATTTGGAAGGTGA+AGG 0.720281 5.2:-42613559 MS.gene053973:CDS
TATGCTAGTGTCAAACAAGG+AGG 0.726500 5.2:-42613754 MS.gene053973:CDS
ACACTCCAATGAGTCCACCA+GGG 0.766435 5.2:-42615712 None:intergenic
AAACTGTACCAACTACCGGG+AGG 0.768178 5.2:+42614054 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATCTTCTTTATTAATT+AGG - chr5.2:42614963-42614982 MS.gene053973:CDS 10.0%
!! TTAATCTTCTTTATTAATTA+GGG - chr5.2:42614964-42614983 MS.gene053973:CDS 10.0%
!! TAATCTTCTTTATTAATTAG+GGG - chr5.2:42614965-42614984 MS.gene053973:CDS 15.0%
!! TTTACTATATCATATTATGT+GGG + chr5.2:42615030-42615049 None:intergenic 15.0%
!!! TTTTACTATATCATATTATG+TGG + chr5.2:42615031-42615050 None:intergenic 15.0%
!! ATAAAGTTAAAGAACATCTA+CGG + chr5.2:42612588-42612607 None:intergenic 20.0%
!! CTGATTAAGATCAAATTAAA+AGG + chr5.2:42615498-42615517 None:intergenic 20.0%
!! TACTATGAATATATATCTCA+AGG - chr5.2:42614869-42614888 MS.gene053973:intron 20.0%
!! TTAACTGATATTCTCAATAT+TGG - chr5.2:42612711-42612730 MS.gene053973:CDS 20.0%
!! TTACTATATCATATTATGTG+GGG + chr5.2:42615029-42615048 None:intergenic 20.0%
! AGGATTTACCTAATTTAATG+GGG - chr5.2:42612433-42612452 MS.gene053973:CDS 25.0%
! ATCATGCTTTAATTGCAAAA+GGG - chr5.2:42612677-42612696 MS.gene053973:CDS 25.0%
! ATGTTGAATTTCGGTATATT+TGG - chr5.2:42613598-42613617 MS.gene053973:CDS 25.0%
! CAGGATTTACCTAATTTAAT+GGG - chr5.2:42612432-42612451 MS.gene053973:CDS 25.0%
! TACGAATAACACATAATTCA+AGG + chr5.2:42615466-42615485 None:intergenic 25.0%
! TATGAATATATATCTCAAGG+TGG - chr5.2:42614872-42614891 MS.gene053973:intron 25.0%
! TCTCAATATTGGTATATTAC+AGG - chr5.2:42612722-42612741 MS.gene053973:CDS 25.0%
! TTATTAATTAGGGGTTAACT+AGG - chr5.2:42614974-42614993 MS.gene053973:CDS 25.0%
!! AAAATATTTTCACTGCACTT+GGG - chr5.2:42612559-42612578 MS.gene053973:intron 25.0%
!! ACATATTTTCAGTCTTAAGA+TGG - chr5.2:42615050-42615069 MS.gene053973:intron 25.0%
!! TAATGACATCCTTGATATTT+TGG + chr5.2:42614848-42614867 None:intergenic 25.0%
!! TAGTTTAAGGTGTAAAGATA+TGG + chr5.2:42613826-42613845 None:intergenic 25.0%
!!! ACTGCTTTTATTCTGAAAAT+TGG + chr5.2:42614797-42614816 None:intergenic 25.0%
!!! GAAATCCTAGTTTTGTTTTT+GGG - chr5.2:42615091-42615110 MS.gene053973:CDS 25.0%
!!! GAATATGAATGTTGTTTTCT+AGG - chr5.2:42614703-42614722 MS.gene053973:CDS 25.0%
!!! TATTGATTCTATTTCTGTGT+TGG - chr5.2:42614386-42614405 MS.gene053973:CDS 25.0%
!!! TGAAATCCTAGTTTTGTTTT+TGG - chr5.2:42615090-42615109 MS.gene053973:CDS 25.0%
ACATAATTCAAGGATAGAGA+TGG + chr5.2:42615456-42615475 None:intergenic 30.0%
ATATCACAATCTATCAGTTG+TGG - chr5.2:42612766-42612785 MS.gene053973:CDS 30.0%
ATATCCGCAATGTTGAATTT+CGG - chr5.2:42613589-42613608 MS.gene053973:CDS 30.0%
ATCAAATTAAAAGGACTTCC+AGG + chr5.2:42615489-42615508 None:intergenic 30.0%
CATACAAACAAGTAAACATG+AGG - chr5.2:42612466-42612485 MS.gene053973:CDS 30.0%
CATCATGCTTTAATTGCAAA+AGG - chr5.2:42612676-42612695 MS.gene053973:CDS 30.0%
GAATGTACATTTATATGGGT+AGG + chr5.2:42615005-42615024 None:intergenic 30.0%
GACAAATTAGAGATAAGAGA+GGG + chr5.2:42615351-42615370 None:intergenic 30.0%
GCAGGATTTACCTAATTTAA+TGG - chr5.2:42612431-42612450 MS.gene053973:CDS 30.0%
GGGAGAATGTACATTTATAT+GGG + chr5.2:42615009-42615028 None:intergenic 30.0%
GTAACGTATCCAAAATATCA+AGG - chr5.2:42614836-42614855 MS.gene053973:intron 30.0%
GTTGTCATTCAAGTGATTTA+TGG - chr5.2:42614760-42614779 MS.gene053973:CDS 30.0%
TATACCGAAATTCAACATTG+CGG + chr5.2:42613596-42613615 None:intergenic 30.0%
TATGATGCTCATAGTATCTA+TGG - chr5.2:42613412-42613431 MS.gene053973:intron 30.0%
TGACAAATTAGAGATAAGAG+AGG + chr5.2:42615352-42615371 None:intergenic 30.0%
TTCAAGAAGTTTAGAAGCTT+AGG + chr5.2:42612908-42612927 None:intergenic 30.0%
TTGCATGATCTCTTGAAAAT+GGG + chr5.2:42613714-42613733 None:intergenic 30.0%
TTTGCATGATCTCTTGAAAA+TGG + chr5.2:42613715-42613734 None:intergenic 30.0%
! AATTATGTGTTATTCGTACC+TGG - chr5.2:42615468-42615487 MS.gene053973:intron 30.0%
! CAAAATATTTTCACTGCACT+TGG - chr5.2:42612558-42612577 MS.gene053973:intron 30.0%
! CTAGAAATGCTTTAGGTATA+AGG - chr5.2:42613788-42613807 MS.gene053973:CDS 30.0%
! GGTAAATTTGCTTTAGACAA+AGG - chr5.2:42614357-42614376 MS.gene053973:CDS 30.0%
! TGATTTTCTCAATCCAAAGA+CGG - chr5.2:42613094-42613113 MS.gene053973:intron 30.0%
! TTCTGAAAATTGGCAGATAT+AGG + chr5.2:42614787-42614806 None:intergenic 30.0%
!! TTCTATTTCTGTGTTGGAAT+TGG - chr5.2:42614392-42614411 MS.gene053973:CDS 30.0%
!! TTTGTTTCGTGTTGTTTGAT+GGG - chr5.2:42615204-42615223 MS.gene053973:CDS 30.0%
!!! AAAGGCCTTTTATTTTGTCA+CGG - chr5.2:42613485-42613504 MS.gene053973:intron 30.0%
!!! TGTTTTCTAGGTTTTTAGCT+AGG - chr5.2:42614715-42614734 MS.gene053973:CDS 30.0%
!!! TTTTGTTTCGTGTTGTTTGA+TGG - chr5.2:42615203-42615222 MS.gene053973:CDS 30.0%
AAACGACGATGAATGATATC+TGG + chr5.2:42615681-42615700 None:intergenic 35.0%
AAATATGCTGCACATTGTAC+TGG - chr5.2:42613529-42613548 MS.gene053973:CDS 35.0%
AAATTCCACTAGATGTGATC+TGG - chr5.2:42612819-42612838 MS.gene053973:CDS 35.0%
AACAACACGAAACAAAAACC+AGG + chr5.2:42615200-42615219 None:intergenic 35.0%
AACATGTGCTTCGTAGTTTA+AGG + chr5.2:42613839-42613858 None:intergenic 35.0%
AAGAACACGATATGTCCAAA+TGG - chr5.2:42613232-42613251 MS.gene053973:intron 35.0%
ACATGTCAAGACAACAACAA+AGG - chr5.2:42614665-42614684 MS.gene053973:CDS 35.0%
ACCTAATTTAATGGGGAGAA+AGG - chr5.2:42612440-42612459 MS.gene053973:CDS 35.0%
ACCTTTCTCCCCATTAAATT+AGG + chr5.2:42612444-42612463 None:intergenic 35.0%
AGAACATCACCAAAACAAGA+TGG - chr5.2:42613290-42613309 MS.gene053973:intron 35.0%
AGATATCATTCATCGTCGTT+TGG - chr5.2:42615680-42615699 MS.gene053973:CDS 35.0%
AGTTATGGTGTATACCAAAG+GGG - chr5.2:42612992-42613011 MS.gene053973:intron 35.0%
ATAGTTCTTCAGTTGGTTGT+GGG + chr5.2:42613660-42613679 None:intergenic 35.0%
ATCTCACTCTACTAGATCAT+GGG - chr5.2:42615287-42615306 MS.gene053973:CDS 35.0%
ATGATCTAGTAGAGTGAGAT+AGG + chr5.2:42615287-42615306 None:intergenic 35.0%
ATTGATAAAGCTCTTGTCTC+GGG + chr5.2:42613747-42613766 None:intergenic 35.0%
ATTGTTGAAATGAGCCAATG+TGG + chr5.2:42615253-42615272 None:intergenic 35.0%
ATTTCTGTGTTGGAATTGGA+TGG - chr5.2:42614396-42614415 MS.gene053973:CDS 35.0%
ATTTGGACATATCGTGTTCT+TGG + chr5.2:42613233-42613252 None:intergenic 35.0%
CAATCTGCTAGAAATGCTTT+AGG - chr5.2:42613781-42613800 MS.gene053973:CDS 35.0%
CACAACATTACTGTTGCTAA+TGG - chr5.2:42615387-42615406 MS.gene053973:intron 35.0%
CAGTTATGGTGTATACCAAA+GGG - chr5.2:42612991-42613010 MS.gene053973:intron 35.0%
CATTGATAAAGCTCTTGTCT+CGG + chr5.2:42613748-42613767 None:intergenic 35.0%
CTATATGCTAGTGTCAAACA+AGG - chr5.2:42614303-42614322 MS.gene053973:CDS 35.0%
CTATTGCATAGTTCTTCAGT+TGG + chr5.2:42613667-42613686 None:intergenic 35.0%
CTGAACCCAAAAACAAAACT+AGG + chr5.2:42615099-42615118 None:intergenic 35.0%
CTGAAGAACTATGCAATAGA+TGG - chr5.2:42613668-42613687 MS.gene053973:CDS 35.0%
GAACATCACCAAAACAAGAT+GGG - chr5.2:42613291-42613310 MS.gene053973:intron 35.0%
GAAGCTTCAAATTTCTGTTC+TGG - chr5.2:42613193-42613212 MS.gene053973:intron 35.0%
GATATCATTCATCGTCGTTT+GGG - chr5.2:42615681-42615700 MS.gene053973:CDS 35.0%
GCATGATGCATAAGAATCTA+AGG + chr5.2:42612664-42612683 None:intergenic 35.0%
GGCTCATTTCAACAATTCTA+CGG - chr5.2:42615257-42615276 MS.gene053973:CDS 35.0%
GGGGAGAATGTACATTTATA+TGG + chr5.2:42615010-42615029 None:intergenic 35.0%
GTAAAAACTTTGCCATGACA+TGG - chr5.2:42614580-42614599 MS.gene053973:CDS 35.0%
GTACTTGATGAAAACATCGT+TGG + chr5.2:42613024-42613043 None:intergenic 35.0%
GTCTTGTATAAGGAAAGAGT+CGG + chr5.2:42615562-42615581 None:intergenic 35.0%
GTCTTTGGATTGAGAAAATC+AGG + chr5.2:42613095-42613114 None:intergenic 35.0%
TAATTTAATGGGGAGAAAGG+TGG - chr5.2:42612443-42612462 MS.gene053973:CDS 35.0%
TACATTGTCATTATTGACCC+TGG - chr5.2:42612953-42612972 MS.gene053973:intron 35.0%
TATCTCACTCTACTAGATCA+TGG - chr5.2:42615286-42615305 MS.gene053973:CDS 35.0%
TCATTATTGACCCTGGAATT+GGG - chr5.2:42612960-42612979 MS.gene053973:intron 35.0%
TCGTTCATATGTCAATCCAT+AGG + chr5.2:42613175-42613194 None:intergenic 35.0%
TTAGATGGAAAGCCACTAAT+TGG - chr5.2:42614447-42614466 MS.gene053973:CDS 35.0%
TTAGCAACAGTAATGTTGTG+AGG + chr5.2:42615387-42615406 None:intergenic 35.0%
TTGAACAAGATACACAGCAT+TGG - chr5.2:42612923-42612942 MS.gene053973:intron 35.0%
TTTGGACATATCGTGTTCTT+GGG + chr5.2:42613232-42613251 None:intergenic 35.0%
! ATGGCAAAGTTTTTACCAAC+AGG + chr5.2:42614576-42614595 None:intergenic 35.0%
! CTTTTCTCAATTCAGTCGTT+CGG - chr5.2:42615157-42615176 MS.gene053973:CDS 35.0%
! GAGACAAGAGCTTTATCAAT+GGG - chr5.2:42613747-42613766 MS.gene053973:CDS 35.0%
! GATGTTTTCATCAAGTACGA+AGG - chr5.2:42613026-42613045 MS.gene053973:intron 35.0%
! GTAATGTTGTGAGGTATCTT+AGG + chr5.2:42615378-42615397 None:intergenic 35.0%
! TACTGTTGCTAATGGTTCTA+AGG - chr5.2:42615395-42615414 MS.gene053973:intron 35.0%
!! ATATTTGGTGTTCCAATGGT+GGG - chr5.2:42613613-42613632 MS.gene053973:CDS 35.0%
!! GCTATTGTTTTGTATCCGAT+TGG + chr5.2:42613358-42613377 None:intergenic 35.0%
!! TATATTTGGTGTTCCAATGG+TGG - chr5.2:42613612-42613631 MS.gene053973:CDS 35.0%
!! TCGTAACATTTGGTGTAAGA+CGG + chr5.2:42613901-42613920 None:intergenic 35.0%
!! TGCAATAGATGGATTGAAGT+TGG - chr5.2:42613679-42613698 MS.gene053973:CDS 35.0%
!! TTGTTTCGTGTTGTTTGATG+GGG - chr5.2:42615205-42615224 MS.gene053973:CDS 35.0%
!!! AGATCATGGGTTTTAGATTC+TGG - chr5.2:42615300-42615319 MS.gene053973:CDS 35.0%
AACCCACTATAATGGTGTCT+TGG - chr5.2:42613387-42613406 MS.gene053973:intron 40.0%
AACGACGATGATCATATGGA+CGG - chr5.2:42612842-42612861 MS.gene053973:CDS 40.0%
AACTACGAAGCACATGTTAG+TGG - chr5.2:42613841-42613860 MS.gene053973:CDS 40.0%
AAGAAAAGTGGTCTCGCAAT+TGG + chr5.2:42613871-42613890 None:intergenic 40.0%
AAGCAGTCTCATGATATCTC+CGG - chr5.2:42613945-42613964 MS.gene053973:CDS 40.0%
AAGGAACGTGGGAGAATTTA+AGG - chr5.2:42613560-42613579 MS.gene053973:CDS 40.0%
AAGGCACAAGTTACTGGTAT+TGG - chr5.2:42615414-42615433 MS.gene053973:intron 40.0%
AAGTTTAGAAGCTTAGGACG+AGG + chr5.2:42612902-42612921 None:intergenic 40.0%
AATGTAACAACGAGCGTGAA+TGG + chr5.2:42614180-42614199 None:intergenic 40.0%
ACAATTCTTCCAATGCAGCA+AGG - chr5.2:42614123-42614142 MS.gene053973:CDS 40.0%
ACACGAAACAAAAACCAGGA+CGG + chr5.2:42615196-42615215 None:intergenic 40.0%
ACGATATGTCCAAATGGAAC+AGG - chr5.2:42613238-42613257 MS.gene053973:intron 40.0%
ACTAGTGCAACCCACTATAA+TGG - chr5.2:42613379-42613398 MS.gene053973:intron 40.0%
ACTCCAAGACACCATTATAG+TGG + chr5.2:42613393-42613412 None:intergenic 40.0%
ACTGTGATGGTAACTTTGAG+AGG - chr5.2:42614540-42614559 MS.gene053973:CDS 40.0%
AGATGCAGAAACACCACAAA+TGG + chr5.2:42615658-42615677 None:intergenic 40.0%
AGGATTCCATCAATGCAGAT+GGG - chr5.2:42612742-42612761 MS.gene053973:CDS 40.0%
AGTAAACTGTACCAACTACC+GGG + chr5.2:42614012-42614031 None:intergenic 40.0%
AGTTTAGAAGCTTAGGACGA+GGG + chr5.2:42612901-42612920 None:intergenic 40.0%
AGTTTGGTCAGAAGTTCAAG+AGG - chr5.2:42614335-42614354 MS.gene053973:CDS 40.0%
ATCAGTTGTGGAAGATGTTG+TGG - chr5.2:42612778-42612797 MS.gene053973:CDS 40.0%
ATTGGGGTTAACTCCAGTTA+TGG - chr5.2:42612977-42612996 MS.gene053973:intron 40.0%
CAATTGTCTTTCCCGTACTT+CGG + chr5.2:42615584-42615603 None:intergenic 40.0%
CATAGTTCTTCAGTTGGTTG+TGG + chr5.2:42613661-42613680 None:intergenic 40.0%
CATTATTGACCCTGGAATTG+GGG - chr5.2:42612961-42612980 MS.gene053973:intron 40.0%
CATTTGGAAGGTGAAGGAAA+TGG - chr5.2:42614507-42614526 MS.gene053973:CDS 40.0%
CCAGAAATGAAGCTTGGAAA+CGG - chr5.2:42614261-42614280 MS.gene053973:CDS 40.0%
CCAGTTATGGTGTATACCAA+AGG - chr5.2:42612990-42613009 MS.gene053973:intron 40.0%
CCTTACAAGATAAACGCTTC+TGG - chr5.2:42613322-42613341 MS.gene053973:intron 40.0%
CCTTATACAAGACCGAAGTA+CGG - chr5.2:42615569-42615588 MS.gene053973:intron 40.0%
CCTTTGGTATACACCATAAC+TGG + chr5.2:42612993-42613012 None:intergenic 40.0%
CTCCAAGACACCATTATAGT+GGG + chr5.2:42613392-42613411 None:intergenic 40.0%
CTTATACAAGACCGAAGTAC+GGG - chr5.2:42615570-42615589 MS.gene053973:intron 40.0%
GAACTTCCAGAAATGAAGCT+TGG - chr5.2:42614255-42614274 MS.gene053973:CDS 40.0%
GAGTAAACTGTACCAACTAC+CGG + chr5.2:42614013-42614032 None:intergenic 40.0%
GGAACAAGATCTCAATCACA+AGG - chr5.2:42615603-42615622 MS.gene053973:intron 40.0%
GGTGTGAATGTTACTACTTC+TGG - chr5.2:42614477-42614496 MS.gene053973:CDS 40.0%
GTCATTATTGACCCTGGAAT+TGG - chr5.2:42612959-42612978 MS.gene053973:intron 40.0%
GTGGACCGTGACAAAATAAA+AGG + chr5.2:42613493-42613512 None:intergenic 40.0%
GTGGCTTCACTTAAGTTAGA+TGG - chr5.2:42614432-42614451 MS.gene053973:CDS 40.0%
GTTATGGTGTATACCAAAGG+GGG - chr5.2:42612993-42613012 MS.gene053973:intron 40.0%
GTTGGTACAGTTTACTCGAT+TGG - chr5.2:42614016-42614035 MS.gene053973:CDS 40.0%
GTTTGGTCAGAAGTTCAAGA+GGG - chr5.2:42614336-42614355 MS.gene053973:CDS 40.0%
TACTTCTGGACATGTGCATT+TGG - chr5.2:42614491-42614510 MS.gene053973:CDS 40.0%
TATGCTAGTGTCAAACAAGG+AGG - chr5.2:42614306-42614325 MS.gene053973:CDS 40.0%
TCAATCCAAAGACGGTATCT+TGG - chr5.2:42613102-42613121 MS.gene053973:intron 40.0%
TCCAAGCAACATATCCATCA+TGG + chr5.2:42613265-42613284 None:intergenic 40.0%
TGAACATTGACCACATGAAG+AGG + chr5.2:42614090-42614109 None:intergenic 40.0%
TGGTGAAAGTAAGACTGTGA+TGG - chr5.2:42614527-42614546 MS.gene053973:CDS 40.0%
TGTTGTGGATAGCTACAAGA+AGG - chr5.2:42612793-42612812 MS.gene053973:CDS 40.0%
TTAAGATGGTATCCAGAGCA+TGG - chr5.2:42615064-42615083 MS.gene053973:intron 40.0%
TTCTTGGACTTGAAGGCAAA+AGG - chr5.2:42613467-42613486 MS.gene053973:intron 40.0%
TTGATAAAGCTCTTGTCTCG+GGG + chr5.2:42613746-42613765 None:intergenic 40.0%
TTTGCCATGACATGGAAGAT+GGG - chr5.2:42614588-42614607 MS.gene053973:CDS 40.0%
! AACAAGGAGGTGTGAAAGTT+TGG - chr5.2:42614319-42614338 MS.gene053973:CDS 40.0%
! ATGTTGTGAGGTATCTTAGG+AGG + chr5.2:42615375-42615394 None:intergenic 40.0%
! CATTGTACTGGTGACAATCA+AGG - chr5.2:42613541-42613560 MS.gene053973:CDS 40.0%
! CGAGACAAGAGCTTTATCAA+TGG - chr5.2:42613746-42613765 MS.gene053973:CDS 40.0%
! CTGTGATGGTAACTTTGAGA+GGG - chr5.2:42614541-42614560 MS.gene053973:CDS 40.0%
! GAAGCGTTTATCTTGTAAGG+AGG + chr5.2:42613322-42613341 None:intergenic 40.0%
! TCTGGAATACAAGCTCCAAT+CGG - chr5.2:42613340-42613359 MS.gene053973:intron 40.0%
! TTGGAATTGGATGGAAGTGT+TGG - chr5.2:42614405-42614424 MS.gene053973:CDS 40.0%
!! AAATGGTGGATGGATGAGAA+TGG + chr5.2:42615641-42615660 None:intergenic 40.0%
!! ACCATGATGGATATGTTGCT+TGG - chr5.2:42613261-42613280 MS.gene053973:intron 40.0%
!! ATTATAGTGGGTTGCACTAG+TGG + chr5.2:42613380-42613399 None:intergenic 40.0%
!! CCAGAAGCGTTTATCTTGTA+AGG + chr5.2:42613325-42613344 None:intergenic 40.0%
!! CGGTATATTTGGTGTTCCAA+TGG - chr5.2:42613609-42613628 MS.gene053973:CDS 40.0%
!! CGTAACATTTGGTGTAAGAC+GGG + chr5.2:42613900-42613919 None:intergenic 40.0%
!! TCAGTCGTTCGGTGTTTAAA+GGG - chr5.2:42615168-42615187 MS.gene053973:CDS 40.0%
!! TTCAGTCGTTCGGTGTTTAA+AGG - chr5.2:42615167-42615186 MS.gene053973:CDS 40.0%
AACCTTTCTTGGCTCAAGTG+TGG - chr5.2:42613051-42613070 MS.gene053973:intron 45.0%
AAGGCTCTTCTTGGACTTGA+AGG - chr5.2:42613460-42613479 MS.gene053973:intron 45.0%
AATGTGGCTGAAGAGGAAAC+TGG + chr5.2:42615237-42615256 None:intergenic 45.0%
ACACCTGACAAGCCAATTAG+TGG + chr5.2:42614462-42614481 None:intergenic 45.0%
ACAGCCTTGATCATTTGGAC+CGG - chr5.2:42612377-42612396 MS.gene053973:CDS 45.0%
ACTCACACCATCACATCAAC+AGG - chr5.2:42614039-42614058 MS.gene053973:CDS 45.0%
ACTTGATGCACCTCTTCATG+TGG - chr5.2:42614077-42614096 MS.gene053973:CDS 45.0%
AGGATTTCAGAACCATGCTC+TGG + chr5.2:42615079-42615098 None:intergenic 45.0%
AGTTCTGCCTGTTGATGTGA+TGG + chr5.2:42614049-42614068 None:intergenic 45.0%
AGTTTCCTCTTCAGCCACAT+TGG - chr5.2:42615236-42615255 MS.gene053973:CDS 45.0%
ATCAATCCACCTTGCTGCAT+TGG + chr5.2:42614135-42614154 None:intergenic 45.0%
ATCCAAAGACGGTATCTTGG+TGG - chr5.2:42613105-42613124 MS.gene053973:intron 45.0%
ATCCCACAGCCTTGATCATT+TGG - chr5.2:42612372-42612391 MS.gene053973:CDS 45.0%
ATTCTTCCAATGCAGCAAGG+TGG - chr5.2:42614126-42614145 MS.gene053973:CDS 45.0%
CAAATGGAACAGGACCATGA+TGG - chr5.2:42613248-42613267 MS.gene053973:intron 45.0%
CAGGATTCCATCAATGCAGA+TGG - chr5.2:42612741-42612760 MS.gene053973:CDS 45.0%
CATATGTCAGAACCCACCAT+TGG + chr5.2:42613628-42613647 None:intergenic 45.0%
CATCATGGTCCTGTTCCATT+TGG + chr5.2:42613250-42613269 None:intergenic 45.0%
CATGAACTCGTACCTGTTGA+CGG - chr5.2:42613149-42613168 MS.gene053973:intron 45.0%
CATGTGCATTTGGAAGGTGA+AGG - chr5.2:42614501-42614520 MS.gene053973:CDS 45.0%
CCGTTTCCAAGCTTCATTTC+TGG + chr5.2:42614264-42614283 None:intergenic 45.0%
CGAAGTACGGGAAAGACAAT+TGG - chr5.2:42615582-42615601 MS.gene053973:intron 45.0%
CTGGAACGACGATGATCATA+TGG - chr5.2:42612838-42612857 MS.gene053973:CDS 45.0%
CTTTGCCATGACATGGAAGA+TGG - chr5.2:42614587-42614606 MS.gene053973:CDS 45.0%
GACGGGAAAGTGAAGAAAAG+TGG + chr5.2:42613883-42613902 None:intergenic 45.0%
GATATCCCCATCTGCATTGA+TGG + chr5.2:42612751-42612770 None:intergenic 45.0%
GCATCAGAAGGAGATGCTAA+AGG - chr5.2:42614210-42614229 MS.gene053973:CDS 45.0%
GGATTCCATCAATGCAGATG+GGG - chr5.2:42612743-42612762 MS.gene053973:CDS 45.0%
GGTGCTTCTGATCATGTTGT+TGG - chr5.2:42615321-42615340 MS.gene053973:intron 45.0%
GTACGAAGGTGAACCTTTCT+TGG - chr5.2:42613040-42613059 MS.gene053973:intron 45.0%
GTACGAGTTCATGAAAGCGA+CGG + chr5.2:42613142-42613161 None:intergenic 45.0%
GTCAGCACTCAGTTTCTACT+CGG - chr5.2:42613922-42613941 MS.gene053973:CDS 45.0%
GTCCAAATGATCAAGGCTGT+GGG + chr5.2:42612377-42612396 None:intergenic 45.0%
TCATCCCATCTTCCATGTCA+TGG + chr5.2:42614595-42614614 None:intergenic 45.0%
TCCAAAGACGGTATCTTGGT+GGG - chr5.2:42613106-42613125 MS.gene053973:intron 45.0%
TCGTTCCAGATCACATCTAG+TGG + chr5.2:42612827-42612846 None:intergenic 45.0%
TCGTTTGGGTCATCCAAGTT+TGG - chr5.2:42615695-42615714 MS.gene053973:CDS 45.0%
TCTCATCCATCCACCATTTG+TGG - chr5.2:42615642-42615661 MS.gene053973:intron 45.0%
TCTGGACATGTGCATTTGGA+AGG - chr5.2:42614495-42614514 MS.gene053973:CDS 45.0%
TGCAGAAACACCACAAATGG+TGG + chr5.2:42615655-42615674 None:intergenic 45.0%
TGGAGTTAACCCCAATTCCA+GGG + chr5.2:42612973-42612992 None:intergenic 45.0%
TGGTGACAATCAAGGAACGT+GGG - chr5.2:42613549-42613568 MS.gene053973:CDS 45.0%
TTAGGACGAGGGTAGTTCTT+TGG + chr5.2:42612890-42612909 None:intergenic 45.0%
TTCAAGGATAGAGATGGCAG+AGG + chr5.2:42615450-42615469 None:intergenic 45.0%
TTGTCACGGTCCACTTATGT+TGG - chr5.2:42613499-42613518 MS.gene053973:intron 45.0%
TTTGCCCGAACCAACATAAG+TGG + chr5.2:42613512-42613531 None:intergenic 45.0%
! ACGGTCCACTTATGTTGGTT+CGG - chr5.2:42613504-42613523 MS.gene053973:CDS 45.0%
! CCGTACTTCGGTCTTGTATA+AGG + chr5.2:42615572-42615591 None:intergenic 45.0%
! GAAACACCACAAATGGTGGA+TGG + chr5.2:42615651-42615670 None:intergenic 45.0%
! GTTTTTCCTTGTTCGAGCAC+CGG + chr5.2:42613967-42613986 None:intergenic 45.0%
!! AAGCCACTAATTGGCTTGTC+AGG - chr5.2:42614456-42614475 MS.gene053973:CDS 45.0%
!! AGGATCGTCCCATCTTGTTT+TGG + chr5.2:42613302-42613321 None:intergenic 45.0%
!! AGTGCTGACGTCGTAACATT+TGG + chr5.2:42613911-42613930 None:intergenic 45.0%
!! ATGGTGGGTTCTGACATATG+TGG - chr5.2:42613628-42613647 MS.gene053973:CDS 45.0%
!! GCAAGGTGGATTGATCTCTA+AGG - chr5.2:42614140-42614159 MS.gene053973:CDS 45.0%
!! GGTTCTAAGGCACAAGTTAC+TGG - chr5.2:42615408-42615427 MS.gene053973:intron 45.0%
!! AGAAAATATAATAAAAATAT+AGG + chr5.2:42615139-42615158 None:intergenic 5.0%
!! GAAAATATAATAAAAATATA+GGG + chr5.2:42615138-42615157 None:intergenic 5.0%
AAACTGTACCAACTACCGGG+AGG + chr5.2:42614009-42614028 None:intergenic 50.0%
ACCCACCAAGATACCGTCTT+TGG + chr5.2:42613110-42613129 None:intergenic 50.0%
AGAGGGTTGAGCATTCCTGT+TGG - chr5.2:42614558-42614577 MS.gene053973:CDS 50.0%
ATATCTCCGGTGCTCGAACA+AGG - chr5.2:42613958-42613977 MS.gene053973:CDS 50.0%
ATGAGCCAATGTGGCTGAAG+AGG + chr5.2:42615244-42615263 None:intergenic 50.0%
CACGCTCGTTGTTACATTCC+CGG - chr5.2:42614182-42614201 MS.gene053973:CDS 50.0%
CTGGAGTTAACCCCAATTCC+AGG + chr5.2:42612974-42612993 None:intergenic 50.0%
CTGGTGACAATCAAGGAACG+TGG - chr5.2:42613548-42613567 MS.gene053973:CDS 50.0%
GCAACTCACAAGGCTCTTCT+TGG - chr5.2:42613451-42613470 MS.gene053973:intron 50.0%
GGTCCAAATGATCAAGGCTG+TGG + chr5.2:42612378-42612397 None:intergenic 50.0%
GTGTTTAAAGGGAACCGTCC+TGG - chr5.2:42615179-42615198 MS.gene053973:CDS 50.0%
TCAATCCATAGGCCGTCAAC+AGG + chr5.2:42613164-42613183 None:intergenic 50.0%
TCCTGCTCAATCAGTCCAAC+CGG + chr5.2:42612417-42612436 None:intergenic 50.0%
TGGCTGAAGAGGAAACTGGT+TGG + chr5.2:42615233-42615252 None:intergenic 50.0%
TTGATCATTTGGACCGGCTG+CGG - chr5.2:42612383-42612402 MS.gene053973:CDS 50.0%
! AAGAGCCTTGTGAGTTGCGA+CGG + chr5.2:42613449-42613468 None:intergenic 50.0%
! ATCGTTGGCTAAACCCCCTT+TGG + chr5.2:42613009-42613028 None:intergenic 50.0%
! TTTGGGTTCAGTCGCTGTCA+AGG - chr5.2:42615108-42615127 MS.gene053973:CDS 50.0%
!! CGGTCCACTTATGTTGGTTC+GGG - chr5.2:42613505-42613524 MS.gene053973:CDS 50.0%
!! GTGAAAGCGTTGTTTCCTCC+CGG - chr5.2:42613991-42614010 MS.gene053973:CDS 50.0%
ACCGGTTGGACTGATTGAGC+AGG - chr5.2:42612413-42612432 MS.gene053973:CDS 55.0%
ATCAAGGCTGTGGGATACCC+TGG + chr5.2:42612368-42612387 None:intergenic 55.0%
CTCGTTGTTACATTCCCGGC+TGG - chr5.2:42614186-42614205 MS.gene053973:CDS 55.0%
GCAATCCGTCGCAACTCACA+AGG - chr5.2:42613441-42613460 MS.gene053973:intron 55.0%
GCAGCCGGTCCAAATGATCA+AGG + chr5.2:42612384-42612403 None:intergenic 55.0%
GGCCACACTTGAGCCAAGAA+AGG + chr5.2:42613056-42613075 None:intergenic 55.0%
TCAGGAAAGTTTACCGCCCC+TGG + chr5.2:42613077-42613096 None:intergenic 55.0%
TCGTACCTGTTGACGGCCTA+TGG - chr5.2:42613156-42613175 MS.gene053973:intron 55.0%
TCTTGGCTCAAGTGTGGCCA+GGG - chr5.2:42613057-42613076 MS.gene053973:intron 55.0%
TGATCATTTGGACCGGCTGC+GGG - chr5.2:42612384-42612403 MS.gene053973:CDS 55.0%
TTCTTGGCTCAAGTGTGGCC+AGG - chr5.2:42613056-42613075 MS.gene053973:intron 55.0%
! CGTTGTTTCCTCCCGGTAGT+TGG - chr5.2:42613998-42614017 MS.gene053973:CDS 55.0%
!! GGATGGAAGTGTTGGAGCAG+TGG - chr5.2:42614413-42614432 MS.gene053973:CDS 55.0%
AAGGCTGTGGGATACCCTGG+TGG + chr5.2:42612365-42612384 None:intergenic 60.0%
CTTGGCTCAAGTGTGGCCAG+GGG - chr5.2:42613058-42613077 MS.gene053973:intron 60.0%
TCTCCTTCTGATGCGCCAGC+CGG + chr5.2:42614204-42614223 None:intergenic 60.0%
ACCGGCTGCGGGACTAACAC+CGG - chr5.2:42612395-42612414 MS.gene053973:CDS 65.0%
CTCCTTCTGATGCGCCAGCC+GGG + chr5.2:42614203-42614222 None:intergenic 65.0%
GCTGCGGGACTAACACCGGT+TGG - chr5.2:42612399-42612418 MS.gene053973:CDS 65.0%
TTCCCGGCTGGCGCATCAGA+AGG - chr5.2:42614198-42614217 MS.gene053973:CDS 65.0%
! ACCGGTGTTAGTCCCGCAGC+CGG + chr5.2:42612399-42612418 None:intergenic 65.0%
GGCTCAAGTGTGGCCAGGGG+CGG - chr5.2:42613061-42613080 MS.gene053973:intron 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 42612356 42615726 42612356 ID=MS.gene053973
chr5.2 mRNA 42612356 42615726 42612356 ID=MS.gene053973.t1;Parent=MS.gene053973
chr5.2 exon 42615666 42615726 42615666 ID=MS.gene053973.t1.exon1;Parent=MS.gene053973.t1
chr5.2 CDS 42615666 42615726 42615666 ID=cds.MS.gene053973.t1;Parent=MS.gene053973.t1
chr5.2 exon 42615084 42615338 42615084 ID=MS.gene053973.t1.exon2;Parent=MS.gene053973.t1
chr5.2 CDS 42615084 42615338 42615084 ID=cds.MS.gene053973.t1;Parent=MS.gene053973.t1
chr5.2 exon 42614925 42615004 42614925 ID=MS.gene053973.t1.exon3;Parent=MS.gene053973.t1
chr5.2 CDS 42614925 42615004 42614925 ID=cds.MS.gene053973.t1;Parent=MS.gene053973.t1
chr5.2 exon 42613503 42614790 42613503 ID=MS.gene053973.t1.exon4;Parent=MS.gene053973.t1
chr5.2 CDS 42613503 42614790 42613503 ID=cds.MS.gene053973.t1;Parent=MS.gene053973.t1
chr5.2 exon 42612600 42612910 42612600 ID=MS.gene053973.t1.exon5;Parent=MS.gene053973.t1
chr5.2 CDS 42612600 42612910 42612600 ID=cds.MS.gene053973.t1;Parent=MS.gene053973.t1
chr5.2 exon 42612356 42612502 42612356 ID=MS.gene053973.t1.exon6;Parent=MS.gene053973.t1
chr5.2 CDS 42612356 42612502 42612356 ID=cds.MS.gene053973.t1;Parent=MS.gene053973.t1
Gene Sequence

>MS.gene053973

ATGAGTCCACCAGGGTATCCCACAGCCTTGATCATTTGGACCGGCTGCGGGACTAACACCGGTTGGACTGATTGAGCAGGATTTACCTAATTTAATGGGGAGAAAGGTGGCATACAAACAAGTAAACATGAGGTGACAAAGTTAAATTCATTGTGCAAAAATGATGAAAGATTCTGTCTACACTTGAGTTTGCTCTTGAAATCAAAATATTTTCACTGCACTTGGGTAGCCGTAGATGTTCTTTAACTTTATTTACATGTTAATGTTGCTACTTCACTTGTCAATTATATGAAAAACGTTGATAACCTTAGATTCTTATGCATCATGCTTTAATTGCAAAAGGGTACATGAAGTTTTAACTGATATTCTCAATATTGGTATATTACAGGATTCCATCAATGCAGATGGGGATATCACAATCTATCAGTTGTGGAAGATGTTGTGGATAGCTACAAGAAGGCTCAAATTCCACTAGATGTGATCTGGAACGACGATGATCATATGGACGGACACAAAGACTTCACACTCAATCCAAAGAACTACCCTCGTCCTAAGCTTCTAAACTTCTTGAACAAGATACACAGCATTGGCATGAAATACATTGTCATTATTGACCCTGGAATTGGGGTTAACTCCAGTTATGGTGTATACCAAAGGGGGTTTAGCCAACGATGTTTTCATCAAGTACGAAGGTGAACCTTTCTTGGCTCAAGTGTGGCCAGGGGCGGTAAACTTTCCTGATTTTCTCAATCCAAAGACGGTATCTTGGTGGGTCGATGAGATCCGTCGCTTTCATGAACTCGTACCTGTTGACGGCCTATGGATTGACATATGAACGAAGCTTCAAATTTCTGTTCTGGTAAATGCAAAATTCCCAAGAACACGATATGTCCAAATGGAACAGGACCATGATGGATATGTTGCTTGGATTGCAAGAACATCACCAAAACAAGATGGGACGATCCTCCTTACAAGATAAACGCTTCTGGAATACAAGCTCCAATCGGATACAAAACAATAGCCACTAGTGCAACCCACTATAATGGTGTCTTGGAGTATGATGCTCATAGTATCTATGGTTTCTCGCAATCCGTCGCAACTCACAAGGCTCTTCTTGGACTTGAAGGCAAAAGGCCTTTTATTTTGTCACGGTCCACTTATGTTGGTTCGGGCAAATATGCTGCACATTGTACTGGTGACAATCAAGGAACGTGGGAGAATTTAAGGTATTCAATATCCGCAATGTTGAATTTCGGTATATTTGGTGTTCCAATGGTGGGTTCTGACATATGTGGATTTTACCCACAACCAACTGAAGAACTATGCAATAGATGGATTGAAGTTGGTGCTTTCTACCCATTTTCAAGAGATCATGCAAACTACTACTCCCCGAGACAAGAGCTTTATCAATGGGATTCAGTAGCTCAATCTGCTAGAAATGCTTTAGGTATAAGGTACACGATTCTACCATATCTTTACACCTTAAACTACGAAGCACATGTTAGTGGATCACCAATTGCGAGACCACTTTTCTTCACTTTCCCGTCTTACACCAAATGTTACGACGTCAGCACTCAGTTTCTACTCGGAAGCAGTCTCATGATATCTCCGGTGCTCGAACAAGGAAAAACACAAGTGAAAGCGTTGTTTCCTCCCGGTAGTTGGTACAGTTTACTCGATTGGACTCACACCATCACATCAACAGGCAGAACTTATGTTACACTTGATGCACCTCTTCATGTGGTCAATGTTCATTTATATCAGAACACAATTCTTCCAATGCAGCAAGGTGGATTGATCTCTAAGGATGCTAGAACGACACCATTCACGCTCGTTGTTACATTCCCGGCTGGCGCATCAGAAGGAGATGCTAAAGGAACACTTTTCATTGACGACGATGAACTTCCAGAAATGAAGCTTGGAAACGGCTATTCGAGTTTTATCGATCTATATGCTAGTGTCAAACAAGGAGGTGTGAAAGTTTGGTCAGAAGTTCAAGAGGGTAAATTTGCTTTAGACAAAGGTTTGATTATTGATTCTATTTCTGTGTTGGAATTGGATGGAAGTGTTGGAGCAGTGGCTTCACTTAAGTTAGATGGAAAGCCACTAATTGGCTTGTCAGGTGTGAATGTTACTACTTCTGGACATGTGCATTTGGAAGGTGAAGGAAATGGTGAAAGTAAGACTGTGATGGTAACTTTGAGAGGGTTGAGCATTCCTGTTGGTAAAAACTTTGCCATGACATGGAAGATGGGATGATTTAGATTGATTGAGATTGTCTCATCTTAGCTTGTTTCTTTTCAATTTTAACATGTCAAGACAACAACAAAGGCTTTTTTTATGAGTTGAATATGAATGTTGTTTTCTAGGTTTTTAGCTAGGTAACTATGAGTTTAAACTAAGTGTTGTCATTCAAGTGATTTATGGTCCTATATCTGCCAATTTTCAGAATAAAAGCAGTCAAATCTAGTATTTATAATGTAACGTATCCAAAATATCAAGGATGTCATTATTACTATGAATATATATCTCAAGGTGGTTGTTGAGATATTGAAAAGTTGTGACTGTATAGTTTTAACTTCACTATTTATTGTATTGATTGTATTGATTAATCTTCTTTATTAATTAGGGGTTAACTAGGAGACTCCTACCCATATAAATGTACATTCTCCCCACATAATATGATATAGTAAAACATATTTTCAGTCTTAAGATGGTATCCAGAGCATGGTTCTGAAATCCTAGTTTTGTTTTTGGGTTCAGTCGCTGTCAAGGCTAGCCCTATATTTTTATTATATTTTCTTTTCTCAATTCAGTCGTTCGGTGTTTAAAGGGAACCGTCCTGGTTTTTGTTTCGTGTTGTTTGATGGGGATCCAACCAGTTTCCTCTTCAGCCACATTGGCTCATTTCAACAATTCTACGGTGTGCCTATCTCACTCTACTAGATCATGGGTTTTAGATTCTGGTGCTTCTGATCATGTTGTTGGTGATCCCTCTCTTATCTCTAATTTGTCACCTCCTAAGATACCTCACAACATTACTGTTGCTAATGGTTCTAAGGCACAAGTTACTGGTATTGGTCAAGCTTCTCCTCTGCCATCTCTATCCTTGAATTATGTGTTATTCGTACCTGGAAGTCCTTTTAATTTGATCTTAATCAGTAAACTTACTCAATCTCTCAATTGTTCTATAACATTTTCTTCCGACTCTTTCCTTATACAAGACCGAAGTACGGGAAAGACAATTGGAACAAGATCTCAATCACAAGGTCTCTATTATCTCCATTCTCATCCATCCACCATTTGTGGTGTTTCTGCATCTCCAGATATCATTCATCGTCGTTTGGGTCATCCAAGTTTGGATAAGTTAA
Protein sequence

>MS.gene053973.t1

MSPPGYPTALIIWTGCGTNTGFHQCRWGYHNLSVVEDVVDSYKKAQIPLDVIWNDDDHMDGHKDFTLNPKNYPRPKLLNFLNKIHSIGMKYIVIIDPGIGVNSSYGAVNFPDFLNPKTVSWWVDEIRRFHELNITKTRWDDPPYKINASGIQAPIGYKTIATSATHYNGVLEYDAHSIYGFSQSVATHKALLGLEGKRPFILSRSTYVGSGKYAAHCTGDNQGTWENLRYSISAMLNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHANYYSPRQELYQWDSVAQSARNALGIRYTILPYLYTLNYEAHVSGSPIARPLFFTFPSYTKCYDVSTQFLLGSSLMISPVLEQGKTQVKALFPPGSWYSLLDWTHTITSTGRTYVTLDAPLHVVNVHLYQNTILPMQQGGLISKDARTTPFTLVVTFPAGASEGDAKGTLFIDDDELPEMKLGNGYSSFIDLYASVKQGGVKVWSEVQEGKFALDKGLIIDSISVLELDGSVGAVASLKLDGKPLIGLSGVNVTTSGHVHLEGEGNGESKTVMVTLRGLSIPVVVRCLKGTVLVFVSCCLMGIQPVSSSATLAHFNNSTVCLSHSTRSWVLDSGASDHVVGDPSLISNLSPPKIPHNITVANGSKAQVTGIGQASPLPSLSLNYVLFTEVRERQLEQDLNHKVSIISILIHPPFVVFLHLQISFIVVWVIQVWIS