Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055015.t1 | XP_004491743.1 | 71.3 | 296 | 83 | 2 | 1 | 296 | 98 | 391 | 4.70E-106 | 394.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055015.t1 | Q8RXN6 | 61.8 | 296 | 111 | 2 | 1 | 296 | 97 | 390 | 8.2e-91 | 335.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055015.t1 | A0A1S2XP41 | 71.3 | 296 | 83 | 2 | 1 | 296 | 98 | 391 | 3.4e-106 | 394.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055015 | MS.gene055058 | 0.800974 | 1.12E-48 | -1.69E-46 |
| MS.gene055015 | MS.gene065039 | 0.801287 | 9.67E-49 | -1.69E-46 |
| MS.gene055015 | MS.gene53827 | 0.817321 | 3.50E-52 | -1.69E-46 |
| MS.gene055015 | MS.gene65003 | 0.824222 | 9.07E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055015.t1 | MTR_7g037560 | 68.525 | 305 | 94 | 2 | 1 | 305 | 36 | 338 | 5.07e-150 | 427 |
| MS.gene055015.t1 | MTR_7g037560 | 68.525 | 305 | 94 | 2 | 1 | 305 | 97 | 399 | 1.48e-149 | 428 |
| MS.gene055015.t1 | MTR_4g070910 | 29.327 | 208 | 146 | 1 | 9 | 216 | 169 | 375 | 1.66e-23 | 100 |
| MS.gene055015.t1 | MTR_5g010260 | 27.885 | 208 | 149 | 1 | 9 | 216 | 169 | 375 | 5.29e-23 | 99.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055015.t1 | AT1G60170 | 59.672 | 305 | 121 | 2 | 1 | 305 | 97 | 399 | 2.56e-109 | 326 |
| MS.gene055015.t1 | AT1G70400 | 52.991 | 117 | 55 | 0 | 1 | 117 | 49 | 165 | 2.98e-34 | 123 |
| MS.gene055015.t1 | AT1G70400 | 55.556 | 108 | 48 | 0 | 1 | 108 | 49 | 156 | 2.62e-33 | 122 |
| MS.gene055015.t1 | AT1G70400 | 55.556 | 108 | 48 | 0 | 1 | 108 | 49 | 156 | 2.29e-32 | 121 |
| MS.gene055015.t1 | AT5G27120 | 30.102 | 196 | 136 | 1 | 21 | 216 | 181 | 375 | 1.83e-21 | 94.7 |
| MS.gene055015.t1 | AT3G05060 | 27.404 | 208 | 150 | 1 | 9 | 216 | 170 | 376 | 5.53e-21 | 93.6 |
| MS.gene055015.t1 | AT5G27140 | 28.342 | 187 | 134 | 0 | 25 | 211 | 157 | 343 | 4.89e-20 | 90.1 |
| MS.gene055015.t1 | AT5G27140 | 28.342 | 187 | 134 | 0 | 25 | 211 | 155 | 341 | 5.35e-20 | 90.1 |
| MS.gene055015.t1 | AT1G56110 | 26.201 | 229 | 147 | 5 | 21 | 236 | 192 | 411 | 2.01e-12 | 67.8 |
| MS.gene055015.t1 | AT3G12860 | 25.108 | 231 | 147 | 5 | 21 | 236 | 192 | 411 | 8.16e-12 | 65.9 |
Find 63 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAATATTGCTTGAAATTTC+CGG | 0.186066 | 7.4:-37263597 | MS.gene055015:CDS |
| GAAGAACAAAATTGTTATTT+TGG | 0.186352 | 7.4:-37263351 | MS.gene055015:CDS |
| CCTAATCTTTCTGCTGTTGT+TGG | 0.274823 | 7.4:-37263302 | MS.gene055015:CDS |
| GCCAGCAGGGTTTATTTGTT+TGG | 0.292378 | 7.4:+37263190 | None:intergenic |
| GGGGACCTGTCGGGGAAAAC+TGG | 0.298409 | 7.4:-37263023 | MS.gene055015:CDS |
| GTTGCAGAAGAGAGCTGTTT+AGG | 0.313833 | 7.4:-37262801 | MS.gene055015:CDS |
| GCCTGCAATGTTCAACTTCT+CGG | 0.315343 | 7.4:-37263215 | MS.gene055015:CDS |
| CAACAACAGCAGAAAGATTA+GGG | 0.321304 | 7.4:+37263303 | None:intergenic |
| CTTGCCCTCATCCATTATAA+TGG | 0.351731 | 7.4:-37263477 | MS.gene055015:CDS |
| ATCAGGCACTCGAAGTGGTT+TGG | 0.359049 | 7.4:+37262932 | None:intergenic |
| CTGTAAGCTCGCATCCTTAG+AGG | 0.372775 | 7.4:+37263095 | None:intergenic |
| AAGTGACACAACCATTATAA+TGG | 0.375964 | 7.4:+37263466 | None:intergenic |
| TCAGGCACTCGAAGTGGTTT+GGG | 0.379928 | 7.4:+37262933 | None:intergenic |
| TCCGAGAAGTTGAACATTGC+AGG | 0.380624 | 7.4:+37263214 | None:intergenic |
| GTTCGTGTCGTTAAGAAGAT+AGG | 0.383033 | 7.4:-37263539 | MS.gene055015:CDS |
| CCAACAACAGCAGAAAGATT+AGG | 0.389586 | 7.4:+37263302 | None:intergenic |
| ATCTCAGGACATGTCAAATC+TGG | 0.393717 | 7.4:+37262764 | None:intergenic |
| AGACTACACCTCCACCTCTA+AGG | 0.401490 | 7.4:-37263109 | MS.gene055015:CDS |
| AGAGAGGTGGTCGTAGGCTT+AGG | 0.405617 | 7.4:-37262890 | MS.gene055015:CDS |
| ATCGACAGGATGATGTACTA+TGG | 0.410265 | 7.4:+37263565 | None:intergenic |
| GAATTGCATCCGATTAGCTT+TGG | 0.420471 | 7.4:+37262827 | None:intergenic |
| AGTGGTTTGGGTCGCTTAGC+AGG | 0.426647 | 7.4:+37262945 | None:intergenic |
| CTTACGTCTTCAACTACATC+GGG | 0.437314 | 7.4:-37263446 | MS.gene055015:CDS |
| TGTACTATGGACTCAAGTTC+CGG | 0.454294 | 7.4:+37263578 | None:intergenic |
| GAATAACTTGAGTTTGCTCC+AGG | 0.464545 | 7.4:+37263132 | None:intergenic |
| GGAAGTCAGCCAAAGCTAAT+CGG | 0.467914 | 7.4:-37262836 | MS.gene055015:CDS |
| TTAAGTACTTCGTTTGAAAG+TGG | 0.486967 | 7.4:+37263419 | None:intergenic |
| TTAATGCATGGGGACCTGTC+GGG | 0.490465 | 7.4:-37263032 | MS.gene055015:CDS |
| AAAGCTAATCGGATGCAATT+CGG | 0.491444 | 7.4:-37262825 | MS.gene055015:CDS |
| AGAAGAATGCAATGGCAGAT+AGG | 0.496400 | 7.4:-37262857 | MS.gene055015:CDS |
| GTTGGAGAAGAGAAGCCAGC+AGG | 0.511323 | 7.4:+37263176 | None:intergenic |
| TTTGCTCCAGGTAACAATCA+TGG | 0.518611 | 7.4:+37263144 | None:intergenic |
| TCCAAACAAATAAACCCTGC+TGG | 0.536547 | 7.4:-37263191 | MS.gene055015:CDS |
| GCTAAACTCATTGTAACAGC+TGG | 0.542896 | 7.4:-37263266 | MS.gene055015:CDS |
| TCCTCACTAAAATTGAAAAG+TGG | 0.546602 | 7.4:-37262980 | MS.gene055015:CDS |
| CAATCATGGAATCGCGACGT+TGG | 0.546641 | 7.4:+37263158 | None:intergenic |
| CACAACCATTATAATGGATG+AGG | 0.548866 | 7.4:+37263472 | None:intergenic |
| TTGGGTCGCTTAGCAGGAGA+GGG | 0.555145 | 7.4:+37262951 | None:intergenic |
| TTTGGGTCGCTTAGCAGGAG+AGG | 0.562931 | 7.4:+37262950 | None:intergenic |
| GCTCGCATCCTTAGAGGTGG+AGG | 0.565029 | 7.4:+37263101 | None:intergenic |
| TAATGCATGGGGACCTGTCG+GGG | 0.565254 | 7.4:-37263031 | MS.gene055015:CDS |
| TAAGTCTACTCGTGCAGCAA+GGG | 0.572657 | 7.4:+37263052 | None:intergenic |
| GCACGAGTAGACTTAATGCA+TGG | 0.572865 | 7.4:-37263044 | MS.gene055015:CDS |
| CTTAATGCATGGGGACCTGT+CGG | 0.578588 | 7.4:-37263033 | MS.gene055015:CDS |
| CACGAGTAGACTTAATGCAT+GGG | 0.580004 | 7.4:-37263043 | MS.gene055015:CDS |
| ACGACACGAACATAATCGAC+AGG | 0.583783 | 7.4:+37263551 | None:intergenic |
| GCGATTCCATGATTGTTACC+TGG | 0.588759 | 7.4:-37263150 | MS.gene055015:CDS |
| TTGGAGAAGAGAAGCCAGCA+GGG | 0.588767 | 7.4:+37263177 | None:intergenic |
| AGAAGTTGAACATTGCAGGC+AGG | 0.595660 | 7.4:+37263218 | None:intergenic |
| AAACTCATTGTAACAGCTGG+TGG | 0.600637 | 7.4:-37263263 | MS.gene055015:CDS |
| AGAAGAAGAGAGGTGGTCGT+AGG | 0.602525 | 7.4:-37262896 | MS.gene055015:CDS |
| TCGTAGGCTTAGGAAAATGA+AGG | 0.605286 | 7.4:-37262880 | MS.gene055015:CDS |
| TTAAGTCTACTCGTGCAGCA+AGG | 0.618694 | 7.4:+37263051 | None:intergenic |
| TCTTGTAAATCAAAGAGAGA+TGG | 0.619850 | 7.4:+37263503 | None:intergenic |
| CTTCAGTGAATCAAGAGCAA+TGG | 0.620212 | 7.4:+37263370 | None:intergenic |
| ACTTACGTCTTCAACTACAT+CGG | 0.620932 | 7.4:-37263447 | MS.gene055015:CDS |
| TAAGCTCGCATCCTTAGAGG+TGG | 0.624518 | 7.4:+37263098 | None:intergenic |
| ACAACCATTATAATGGATGA+GGG | 0.637450 | 7.4:+37263473 | None:intergenic |
| TCAGCATCAGGCACTCGAAG+TGG | 0.656951 | 7.4:+37262927 | None:intergenic |
| AATGAAGGAGAAGAATGCAA+TGG | 0.688085 | 7.4:-37262865 | MS.gene055015:CDS |
| ACGAGTAGACTTAATGCATG+GGG | 0.715496 | 7.4:-37263042 | MS.gene055015:CDS |
| AACAACAGCAGAAAGATTAG+GGG | 0.715843 | 7.4:+37263304 | None:intergenic |
| TTACGTCTTCAACTACATCG+GGG | 0.787026 | 7.4:-37263445 | MS.gene055015:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAGAACAAAATTGTTATTT+TGG | - | chr7.4:37263081-37263100 | MS.gene055015:CDS | 20.0% |
| ! | CAAATATTGCTTGAAATTTC+CGG | - | chr7.4:37262835-37262854 | MS.gene055015:CDS | 25.0% |
| AAGTGACACAACCATTATAA+TGG | + | chr7.4:37262969-37262988 | None:intergenic | 30.0% | |
| TCCTCACTAAAATTGAAAAG+TGG | - | chr7.4:37263452-37263471 | MS.gene055015:CDS | 30.0% | |
| TCTTGTAAATCAAAGAGAGA+TGG | + | chr7.4:37262932-37262951 | None:intergenic | 30.0% | |
| TTAAGTACTTCGTTTGAAAG+TGG | + | chr7.4:37263016-37263035 | None:intergenic | 30.0% | |
| !! | ACAACCATTATAATGGATGA+GGG | + | chr7.4:37262962-37262981 | None:intergenic | 30.0% |
| AAAGCTAATCGGATGCAATT+CGG | - | chr7.4:37263607-37263626 | MS.gene055015:CDS | 35.0% | |
| AACAACAGCAGAAAGATTAG+GGG | + | chr7.4:37263131-37263150 | None:intergenic | 35.0% | |
| AATGAAGGAGAAGAATGCAA+TGG | - | chr7.4:37263567-37263586 | MS.gene055015:CDS | 35.0% | |
| ACTTACGTCTTCAACTACAT+CGG | - | chr7.4:37262985-37263004 | MS.gene055015:CDS | 35.0% | |
| CAACAACAGCAGAAAGATTA+GGG | + | chr7.4:37263132-37263151 | None:intergenic | 35.0% | |
| !! | CACAACCATTATAATGGATG+AGG | + | chr7.4:37262963-37262982 | None:intergenic | 35.0% |
| !!! | GCCACTTTTCAATTTTAGTG+AGG | + | chr7.4:37263456-37263475 | None:intergenic | 35.0% |
| AAACTCATTGTAACAGCTGG+TGG | - | chr7.4:37263169-37263188 | MS.gene055015:CDS | 40.0% | |
| ACGAGTAGACTTAATGCATG+GGG | - | chr7.4:37263390-37263409 | MS.gene055015:CDS | 40.0% | |
| AGAAGAATGCAATGGCAGAT+AGG | - | chr7.4:37263575-37263594 | MS.gene055015:CDS | 40.0% | |
| ATCGACAGGATGATGTACTA+TGG | + | chr7.4:37262870-37262889 | None:intergenic | 40.0% | |
| CACGAGTAGACTTAATGCAT+GGG | - | chr7.4:37263389-37263408 | MS.gene055015:CDS | 40.0% | |
| CCAACAACAGCAGAAAGATT+AGG | + | chr7.4:37263133-37263152 | None:intergenic | 40.0% | |
| CCTAAAAAGAAGAAGAGAGG+TGG | - | chr7.4:37263529-37263548 | MS.gene055015:CDS | 40.0% | |
| CCTAATCTTTCTGCTGTTGT+TGG | - | chr7.4:37263130-37263149 | MS.gene055015:CDS | 40.0% | |
| CTTACGTCTTCAACTACATC+GGG | - | chr7.4:37262986-37263005 | MS.gene055015:CDS | 40.0% | |
| CTTCAGTGAATCAAGAGCAA+TGG | + | chr7.4:37263065-37263084 | None:intergenic | 40.0% | |
| CTTGCCCTCATCCATTATAA+TGG | - | chr7.4:37262955-37262974 | MS.gene055015:CDS | 40.0% | |
| GAGCCTAAAAAGAAGAAGAG+AGG | - | chr7.4:37263526-37263545 | MS.gene055015:CDS | 40.0% | |
| GCTAAACTCATTGTAACAGC+TGG | - | chr7.4:37263166-37263185 | MS.gene055015:CDS | 40.0% | |
| TCCAAACAAATAAACCCTGC+TGG | - | chr7.4:37263241-37263260 | MS.gene055015:CDS | 40.0% | |
| TCGTAGGCTTAGGAAAATGA+AGG | - | chr7.4:37263552-37263571 | MS.gene055015:CDS | 40.0% | |
| TGTACTATGGACTCAAGTTC+CGG | + | chr7.4:37262857-37262876 | None:intergenic | 40.0% | |
| TTACGTCTTCAACTACATCG+GGG | - | chr7.4:37262987-37263006 | MS.gene055015:CDS | 40.0% | |
| TTTGCTCCAGGTAACAATCA+TGG | + | chr7.4:37263291-37263310 | None:intergenic | 40.0% | |
| ! | CCACCTCTCTTCTTCTTTTT+AGG | + | chr7.4:37263532-37263551 | None:intergenic | 40.0% |
| ! | GAATAACTTGAGTTTGCTCC+AGG | + | chr7.4:37263303-37263322 | None:intergenic | 40.0% |
| ! | GTTCGTGTCGTTAAGAAGAT+AGG | - | chr7.4:37262893-37262912 | MS.gene055015:CDS | 40.0% |
| !! | GAATTGCATCCGATTAGCTT+TGG | + | chr7.4:37263608-37263627 | None:intergenic | 40.0% |
| !! | TTCTTTTTAGGCTCAGCATC+AGG | + | chr7.4:37263520-37263539 | None:intergenic | 40.0% |
| ACGACACGAACATAATCGAC+AGG | + | chr7.4:37262884-37262903 | None:intergenic | 45.0% | |
| AGAAGTTGAACATTGCAGGC+AGG | + | chr7.4:37263217-37263236 | None:intergenic | 45.0% | |
| GCACGAGTAGACTTAATGCA+TGG | - | chr7.4:37263388-37263407 | MS.gene055015:CDS | 45.0% | |
| GCCAGCAGGGTTTATTTGTT+TGG | + | chr7.4:37263245-37263264 | None:intergenic | 45.0% | |
| GCCTGCAATGTTCAACTTCT+CGG | - | chr7.4:37263217-37263236 | MS.gene055015:CDS | 45.0% | |
| GCGATTCCATGATTGTTACC+TGG | - | chr7.4:37263282-37263301 | MS.gene055015:CDS | 45.0% | |
| GGAAGTCAGCCAAAGCTAAT+CGG | - | chr7.4:37263596-37263615 | MS.gene055015:CDS | 45.0% | |
| GTTGCAGAAGAGAGCTGTTT+AGG | - | chr7.4:37263631-37263650 | MS.gene055015:CDS | 45.0% | |
| TAAGTCTACTCGTGCAGCAA+GGG | + | chr7.4:37263383-37263402 | None:intergenic | 45.0% | |
| TCCGAGAAGTTGAACATTGC+AGG | + | chr7.4:37263221-37263240 | None:intergenic | 45.0% | |
| TTAAGTCTACTCGTGCAGCA+AGG | + | chr7.4:37263384-37263403 | None:intergenic | 45.0% | |
| AGAAGAAGAGAGGTGGTCGT+AGG | - | chr7.4:37263536-37263555 | MS.gene055015:CDS | 50.0% | |
| AGACTACACCTCCACCTCTA+AGG | - | chr7.4:37263323-37263342 | MS.gene055015:CDS | 50.0% | |
| ATCAGGCACTCGAAGTGGTT+TGG | + | chr7.4:37263503-37263522 | None:intergenic | 50.0% | |
| CAATCATGGAATCGCGACGT+TGG | + | chr7.4:37263277-37263296 | None:intergenic | 50.0% | |
| CTGTAAGCTCGCATCCTTAG+AGG | + | chr7.4:37263340-37263359 | None:intergenic | 50.0% | |
| CTTAATGCATGGGGACCTGT+CGG | - | chr7.4:37263399-37263418 | MS.gene055015:CDS | 50.0% | |
| TAAGCTCGCATCCTTAGAGG+TGG | + | chr7.4:37263337-37263356 | None:intergenic | 50.0% | |
| TCAGGCACTCGAAGTGGTTT+GGG | + | chr7.4:37263502-37263521 | None:intergenic | 50.0% | |
| TTAATGCATGGGGACCTGTC+GGG | - | chr7.4:37263400-37263419 | MS.gene055015:CDS | 50.0% | |
| TTGGAGAAGAGAAGCCAGCA+GGG | + | chr7.4:37263258-37263277 | None:intergenic | 50.0% | |
| ! | TTGTTCCAGTTTTCCCCGAC+AGG | + | chr7.4:37263417-37263436 | None:intergenic | 50.0% |
| AGAGAGGTGGTCGTAGGCTT+AGG | - | chr7.4:37263542-37263561 | MS.gene055015:CDS | 55.0% | |
| GTTGGAGAAGAGAAGCCAGC+AGG | + | chr7.4:37263259-37263278 | None:intergenic | 55.0% | |
| TAATGCATGGGGACCTGTCG+GGG | - | chr7.4:37263401-37263420 | MS.gene055015:CDS | 55.0% | |
| TCAGCATCAGGCACTCGAAG+TGG | + | chr7.4:37263508-37263527 | None:intergenic | 55.0% | |
| TTGGGTCGCTTAGCAGGAGA+GGG | + | chr7.4:37263484-37263503 | None:intergenic | 55.0% | |
| TTTGGGTCGCTTAGCAGGAG+AGG | + | chr7.4:37263485-37263504 | None:intergenic | 55.0% | |
| ! | AGTGGTTTGGGTCGCTTAGC+AGG | + | chr7.4:37263490-37263509 | None:intergenic | 55.0% |
| GCTCGCATCCTTAGAGGTGG+AGG | + | chr7.4:37263334-37263353 | None:intergenic | 60.0% | |
| GGGGACCTGTCGGGGAAAAC+TGG | - | chr7.4:37263409-37263428 | MS.gene055015:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 37262767 | 37263687 | 37262767 | ID=MS.gene055015 |
| chr7.4 | mRNA | 37262767 | 37263687 | 37262767 | ID=MS.gene055015.t1;Parent=MS.gene055015 |
| chr7.4 | exon | 37262767 | 37263687 | 37262767 | ID=MS.gene055015.t1.exon1;Parent=MS.gene055015.t1 |
| chr7.4 | CDS | 37262767 | 37263687 | 37262767 | ID=cds.MS.gene055015.t1;Parent=MS.gene055015.t1 |
| Gene Sequence |
| Protein sequence |
