Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055051.t1 | RHN77599.1 | 95.8 | 119 | 5 | 0 | 1 | 119 | 207 | 325 | 6.90E-53 | 216.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055051.t1 | Q54UB1 | 30.9 | 94 | 62 | 2 | 1 | 91 | 217 | 310 | 3.2e-06 | 52.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055051.t1 | G7I755 | 95.8 | 119 | 5 | 0 | 1 | 119 | 208 | 326 | 5.0e-53 | 216.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055051 | MS.gene30263 | 0.942812 | 3.70E-102 | -1.69E-46 |
| MS.gene055051 | MS.gene92557 | 0.808834 | 2.55E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055051.t1 | MTR_1g024920 | 95.798 | 119 | 5 | 0 | 1 | 119 | 208 | 326 | 1.73e-74 | 228 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055051.t1 | AT2G45730 | 50.000 | 118 | 56 | 2 | 1 | 115 | 205 | 322 | 2.52e-31 | 115 |
Find 24 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGTTGGTGGCCTTCTTAC+TGG | 0.198600 | 7.4:+37877116 | MS.gene055051:CDS |
| ATCTCTCATTGGTCAGAAAA+TGG | 0.252753 | 7.4:+37878239 | MS.gene055051:CDS |
| AAGTGCAATTTACATTTAAT+TGG | 0.266218 | 7.4:-37878118 | None:intergenic |
| TTTGTATGCAATTCATATCT+TGG | 0.278999 | 7.4:+37877418 | MS.gene055051:CDS |
| ACACTGCCGTCATCATGTTT+TGG | 0.317670 | 7.4:-37878089 | None:intergenic |
| TGACATTCTCGTTGTTGATA+TGG | 0.336210 | 7.4:+37877097 | MS.gene055051:CDS |
| GGTGCAGTGGCAAAGCGTTT+AGG | 0.381408 | 7.4:+37877137 | MS.gene055051:CDS |
| TTGTGACAACAAGTCAGAAA+TGG | 0.435540 | 7.4:-37878045 | None:intergenic |
| TTATTTATTGCAGAATTGTA+AGG | 0.438615 | 7.4:+37878018 | MS.gene055051:intron |
| CTCGTTGTTGATATGGTTGG+TGG | 0.492274 | 7.4:+37877104 | MS.gene055051:CDS |
| ATTCTCGTTGTTGATATGGT+TGG | 0.536995 | 7.4:+37877101 | MS.gene055051:CDS |
| TCTTGGACATGCACCATCCA+TGG | 0.538347 | 7.4:+37877435 | MS.gene055051:CDS |
| TTGCCACTGCACCAGTAAGA+AGG | 0.543209 | 7.4:-37877127 | None:intergenic |
| GCATGGAGGAAATCTCTCAT+TGG | 0.552511 | 7.4:+37878228 | MS.gene055051:CDS |
| AAATATCCTCACAATATCCA+TGG | 0.553785 | 7.4:-37877452 | None:intergenic |
| TGGCCTTCTTACTGGTGCAG+TGG | 0.592640 | 7.4:+37877124 | MS.gene055051:CDS |
| GCAGTGGCAAAGCGTTTAGG+AGG | 0.600305 | 7.4:+37877140 | MS.gene055051:CDS |
| ATCCTCACAATATCCATGGA+TGG | 0.603371 | 7.4:-37877448 | None:intergenic |
| CACCATCCATGGATATTGTG+AGG | 0.613262 | 7.4:+37877446 | MS.gene055051:CDS |
| CAAAATCCAAAACATGATGA+CGG | 0.624523 | 7.4:+37878083 | MS.gene055051:CDS |
| AATGTGTGCATCAGTCAGCA+TGG | 0.631729 | 7.4:+37878211 | MS.gene055051:CDS |
| TAAGCGATGAAATCTGCAAG+AGG | 0.636208 | 7.4:+37877479 | MS.gene055051:CDS |
| AAAACATGATGACGGCAGTG+TGG | 0.681492 | 7.4:+37878091 | MS.gene055051:CDS |
| GTGTGCATCAGTCAGCATGG+AGG | 0.745962 | 7.4:+37878214 | MS.gene055051:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGCTTTTTTTTTTTTTAATA+TGG | + | chr7.4:37877201-37877220 | MS.gene055051:intron | 10.0% |
| !!! | TTTTAACTCAAAAAAATAAT+TGG | - | chr7.4:37877532-37877551 | None:intergenic | 10.0% |
| !! | AAATGTTTATAGGTTTCTAT+CGG | + | chr7.4:37877791-37877810 | MS.gene055051:intron | 20.0% |
| !! | AAGTGCAATTTACATTTAAT+TGG | - | chr7.4:37878121-37878140 | None:intergenic | 20.0% |
| !! | AATCAAAGAGAAATGTTTAT+AGG | + | chr7.4:37877781-37877800 | MS.gene055051:intron | 20.0% |
| !! | TTATTTATTGCAGAATTGTA+AGG | + | chr7.4:37878018-37878037 | MS.gene055051:intron | 20.0% |
| ! | AATGTTTATAGGTTTCTATC+GGG | + | chr7.4:37877792-37877811 | MS.gene055051:intron | 25.0% |
| ! | ATACCTATTAATTCAACACA+TGG | - | chr7.4:37877822-37877841 | None:intergenic | 25.0% |
| ! | TTAACTAAATGATTCATCAG+AGG | - | chr7.4:37877904-37877923 | None:intergenic | 25.0% |
| ! | TTCCATTCTTAACAATCTTT+GGG | + | chr7.4:37877303-37877322 | MS.gene055051:intron | 25.0% |
| ! | TTTCCATTCTTAACAATCTT+TGG | + | chr7.4:37877302-37877321 | MS.gene055051:intron | 25.0% |
| ! | TTTGTATGCAATTCATATCT+TGG | + | chr7.4:37877418-37877437 | MS.gene055051:CDS | 25.0% |
| !! | ATCTAACGTTAATGTTTTAC+AGG | + | chr7.4:37877388-37877407 | MS.gene055051:intron | 25.0% |
| !!! | GAAAAATGGAAGCTTTAAAA+AGG | - | chr7.4:37877630-37877649 | None:intergenic | 25.0% |
| !!! | TCCAAACTATTTCTGAAAAA+TGG | - | chr7.4:37877644-37877663 | None:intergenic | 25.0% |
| !!! | TCCATTTTTCAGAAATAGTT+TGG | + | chr7.4:37877640-37877659 | MS.gene055051:intron | 25.0% |
| !!! | TGAAGTTTTGTTTCTTTTGT+CGG | + | chr7.4:37877977-37877996 | MS.gene055051:intron | 25.0% |
| AAATATCCTCACAATATCCA+TGG | - | chr7.4:37877455-37877474 | None:intergenic | 30.0% | |
| CAAAATCCAAAACATGATGA+CGG | + | chr7.4:37878083-37878102 | MS.gene055051:CDS | 30.0% | |
| CACGACATATGATAAATTGT+TGG | - | chr7.4:37878177-37878196 | None:intergenic | 30.0% | |
| GATAAATTGTTGGTACAGTA+AGG | - | chr7.4:37878167-37878186 | None:intergenic | 30.0% | |
| GTTTGGATCACTCTTTATAT+TGG | + | chr7.4:37877657-37877676 | MS.gene055051:intron | 30.0% | |
| TAGCCATGTGTTGAATTAAT+AGG | + | chr7.4:37877816-37877835 | MS.gene055051:intron | 30.0% | |
| TTTCTCTTTCAGTGAATCAA+CGG | - | chr7.4:37877600-37877619 | None:intergenic | 30.0% | |
| ! | CTTCAATTTTGTATGGTGAT+TGG | - | chr7.4:37877878-37877897 | None:intergenic | 30.0% |
| ! | GAGGATACTTCAATTTTGTA+TGG | - | chr7.4:37877885-37877904 | None:intergenic | 30.0% |
| ! | TGCATCTAGAGTTATTCATT+TGG | - | chr7.4:37877725-37877744 | None:intergenic | 30.0% |
| !!! | GAAGTTTTGTTTCTTTTGTC+GGG | + | chr7.4:37877978-37877997 | MS.gene055051:intron | 30.0% |
| ATCTCTCATTGGTCAGAAAA+TGG | + | chr7.4:37878239-37878258 | MS.gene055051:CDS | 35.0% | |
| ATTCTCGTTGTTGATATGGT+TGG | + | chr7.4:37877101-37877120 | MS.gene055051:CDS | 35.0% | |
| CACTTCCTTGATCTTCTAAT+CGG | + | chr7.4:37878136-37878155 | MS.gene055051:intron | 35.0% | |
| GACCCAAAGATTGTTAAGAA+TGG | - | chr7.4:37877308-37877327 | None:intergenic | 35.0% | |
| GATCACTCTTTATATTGGCA+TGG | + | chr7.4:37877662-37877681 | MS.gene055051:intron | 35.0% | |
| TAGAACCGATTAGAAGATCA+AGG | - | chr7.4:37878144-37878163 | None:intergenic | 35.0% | |
| TGACATTCTCGTTGTTGATA+TGG | + | chr7.4:37877097-37877116 | MS.gene055051:CDS | 35.0% | |
| TTGTGACAACAAGTCAGAAA+TGG | - | chr7.4:37878048-37878067 | None:intergenic | 35.0% | |
| !!! | AGTGTTGGTTTTCATAGTTC+AGG | + | chr7.4:37877339-37877358 | MS.gene055051:intron | 35.0% |
| ATCCTCACAATATCCATGGA+TGG | - | chr7.4:37877451-37877470 | None:intergenic | 40.0% | |
| CAGGAGCTAATACATGCATT+AGG | + | chr7.4:37877358-37877377 | MS.gene055051:intron | 40.0% | |
| TAAGCGATGAAATCTGCAAG+AGG | + | chr7.4:37877479-37877498 | MS.gene055051:CDS | 40.0% | |
| ! | CGTTAATGTTTTACAGGCAC+TGG | + | chr7.4:37877394-37877413 | MS.gene055051:intron | 40.0% |
| !! | GGTCTAGTGATTGATAGTGT+TGG | + | chr7.4:37877324-37877343 | MS.gene055051:intron | 40.0% |
| AATGTGTGCATCAGTCAGCA+TGG | + | chr7.4:37878211-37878230 | MS.gene055051:CDS | 45.0% | |
| CACCATCCATGGATATTGTG+AGG | + | chr7.4:37877446-37877465 | MS.gene055051:CDS | 45.0% | |
| CTCGTTGTTGATATGGTTGG+TGG | + | chr7.4:37877104-37877123 | MS.gene055051:CDS | 45.0% | |
| GCATGGAGGAAATCTCTCAT+TGG | + | chr7.4:37878228-37878247 | MS.gene055051:CDS | 45.0% | |
| ! | ACACTGCCGTCATCATGTTT+TGG | - | chr7.4:37878092-37878111 | None:intergenic | 45.0% |
| !! | AAAACATGATGACGGCAGTG+TGG | + | chr7.4:37878091-37878110 | MS.gene055051:CDS | 45.0% |
| TCTTGGACATGCACCATCCA+TGG | + | chr7.4:37877435-37877454 | MS.gene055051:CDS | 50.0% | |
| TTGCCACTGCACCAGTAAGA+AGG | - | chr7.4:37877130-37877149 | None:intergenic | 50.0% | |
| !! | ATGGTTGGTGGCCTTCTTAC+TGG | + | chr7.4:37877116-37877135 | MS.gene055051:CDS | 50.0% |
| GTGTGCATCAGTCAGCATGG+AGG | + | chr7.4:37878214-37878233 | MS.gene055051:CDS | 55.0% | |
| TGGCCTTCTTACTGGTGCAG+TGG | + | chr7.4:37877124-37877143 | MS.gene055051:CDS | 55.0% | |
| ! | GCAGTGGCAAAGCGTTTAGG+AGG | + | chr7.4:37877140-37877159 | MS.gene055051:CDS | 55.0% |
| ! | GGTGCAGTGGCAAAGCGTTT+AGG | + | chr7.4:37877137-37877156 | MS.gene055051:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 37877074 | 37878292 | 37877074 | ID=MS.gene055051 |
| chr7.4 | mRNA | 37877074 | 37878292 | 37877074 | ID=MS.gene055051.t1;Parent=MS.gene055051 |
| chr7.4 | exon | 37877074 | 37877161 | 37877074 | ID=MS.gene055051.t1.exon1;Parent=MS.gene055051.t1 |
| chr7.4 | CDS | 37877074 | 37877161 | 37877074 | ID=cds.MS.gene055051.t1;Parent=MS.gene055051.t1 |
| chr7.4 | exon | 37877410 | 37877500 | 37877410 | ID=MS.gene055051.t1.exon2;Parent=MS.gene055051.t1 |
| chr7.4 | CDS | 37877410 | 37877500 | 37877410 | ID=cds.MS.gene055051.t1;Parent=MS.gene055051.t1 |
| chr7.4 | exon | 37878031 | 37878127 | 37878031 | ID=MS.gene055051.t1.exon3;Parent=MS.gene055051.t1 |
| chr7.4 | CDS | 37878031 | 37878127 | 37878031 | ID=cds.MS.gene055051.t1;Parent=MS.gene055051.t1 |
| chr7.4 | exon | 37878206 | 37878292 | 37878206 | ID=MS.gene055051.t1.exon4;Parent=MS.gene055051.t1 |
| chr7.4 | CDS | 37878206 | 37878292 | 37878206 | ID=cds.MS.gene055051.t1;Parent=MS.gene055051.t1 |
| Gene Sequence |
| Protein sequence |
