AlfalfaGEDB | MS.gene055131

M. sativa cultivar XinJiangDaYe / MS.gene055131

KEGG: K14686
GO: GO:0000041 GO:0003674 GO:0005215 GO:0005375 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016021 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0034220 GO:0035434 GO:0046873 GO:0046915 GO:0051179 GO:0051234 GO:0055085 GO:0098655 GO:0098660 GO:0098662 GO:0110165
PFAM: PF04145.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055131.t1	XP_013443255.1	96	149	6	0	1	149	1	149	4.40E-73	283.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055131.t1	Q8GWP3	45.0	129	69	1	12	140	10	136	6.5e-25	115.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055131.t1	A0A072TUQ5	96.0	149	6	0	1	149	1	149	3.1e-73	283.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055131	MS.gene75220	0.803807	2.92E-49	-1.69E-46
MS.gene055131	MS.gene84497	0.811352	7.31E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055131.t1	MTR_0027s0220	95.973	149	6	0	1	149	1	149	5.22e-102	288
MS.gene055131.t1	MTR_4g065660	54.545	99	42	2	30	128	35	130	4.62e-28	101
MS.gene055131.t1	MTR_4g064963	55.446	101	42	2	30	130	46	143	6.65e-28	101
MS.gene055131.t1	MTR_4g065123	49.038	104	49	2	27	130	28	127	1.47e-26	97.4
MS.gene055131.t1	MTR_7g066070	45.217	115	60	2	27	141	22	133	4.53e-25	94.0
MS.gene055131.t1	MTR_4g019870	41.270	126	71	2	22	147	8	130	2.51e-24	91.7
MS.gene055131.t1	MTR_1g015000	31.690	142	72	2	27	147	2	139	4.54e-16	70.5
MS.gene055131.t1	MTR_3g105330	31.429	140	67	4	27	145	2	133	3.15e-14	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055131.t1	AT5G59030	45.000	120	66	0	30	149	45	164	7.00e-34	117
MS.gene055131.t1	AT2G26975	44.961	129	69	1	12	140	10	136	1.65e-33	115
MS.gene055131.t1	AT2G37925	47.826	115	58	2	27	140	30	143	1.06e-31	110
MS.gene055131.t1	AT3G46900	47.273	110	56	2	31	140	34	141	8.50e-31	108
MS.gene055131.t1	AT5G59040	48.246	114	56	1	27	140	30	140	1.42e-28	103
MS.gene055131.t1	AT5G20650	34.028	144	68	4	27	148	2	140	1.19e-15	69.7

Find 48 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGCCATGTTATTGGGTTTA+TGG	0.163011	7.4:+39161613	MS.gene055131:CDS
GCAGTTGGTGGCCATGTTAT+TGG	0.206160	7.4:+39161605	MS.gene055131:CDS
CGATCCACACATCATTCTAT+TGG	0.284405	7.4:+39161324	MS.gene055131:CDS
TTGGCTGTTATGTCGTTTAA+TGG	0.294096	7.4:+39161566	MS.gene055131:CDS
CAGTTGGTGGCCATGTTATT+GGG	0.316028	7.4:+39161606	MS.gene055131:CDS
GATCCACACATCATTCTATT+GGG	0.329197	7.4:+39161325	MS.gene055131:CDS
TCCACGGTGGCGACGATGTT+GGG	0.338831	7.4:+39161287	MS.gene055131:CDS
CCGTACTGGATTCTCGTACT+TGG	0.410577	7.4:+39161538	MS.gene055131:CDS
TACTATTCCATTGCTGGCCC+GGG	0.418443	7.4:+39161363	MS.gene055131:CDS
GGGCTGTACGGTGTCCGTAC+TGG	0.427912	7.4:+39161524	MS.gene055131:CDS
TAGACATACTATTCCATTGC+TGG	0.428991	7.4:+39161357	MS.gene055131:CDS
CTCCACGGTGGCGACGATGT+TGG	0.436829	7.4:+39161286	MS.gene055131:CDS
ATACTATTCCATTGCTGGCC+CGG	0.443048	7.4:+39161362	MS.gene055131:CDS
CGCTGTCCCCGGGCCAGCAA+TGG	0.456744	7.4:-39161370	None:intergenic
CATCGTCGCCACCGTGGAGT+TGG	0.477324	7.4:-39161282	None:intergenic
GTACATGACAGCGCTGTCCC+CGG	0.489328	7.4:-39161381	None:intergenic
ATTCTCGTACTTGGTTATGT+TGG	0.491065	7.4:+39161547	MS.gene055131:CDS
AGGTTTGAGTTTAACAATGT+TGG	0.499385	7.4:-39161462	None:intergenic
GTGTTTCTTGCTGCAGTTGG+TGG	0.500610	7.4:+39161593	MS.gene055131:CDS
GGTGTGTTTCTTGCTGCAGT+TGG	0.502035	7.4:+39161590	MS.gene055131:CDS
GTTTGTAATGTCGGTGTTGG+TGG	0.507829	7.4:+39161424	MS.gene055131:CDS
ACGACGTCGTTTGATGTCCC+AGG	0.512723	7.4:-39161482	None:intergenic
TTGTTCACCACCACTGCAAA+TGG	0.515915	7.4:+39161251	MS.gene055131:CDS
AACATTGTTAAACTCAAACC+TGG	0.522755	7.4:+39161464	MS.gene055131:CDS
CGTGTTTGTAATGTCGGTGT+TGG	0.532961	7.4:+39161421	MS.gene055131:CDS
TACATGACAGCGCTGTCCCC+GGG	0.550555	7.4:-39161380	None:intergenic
CGGTGGCGACGATGTTGGGC+CGG	0.564945	7.4:+39161291	MS.gene055131:CDS
TGTGGATCGGAATTCTTCGC+CGG	0.568160	7.4:-39161310	None:intergenic
GATCCTCGTGTTTGTAATGT+CGG	0.579313	7.4:+39161415	MS.gene055131:CDS
TAATGTCGGTGTTGGTGGAG+TGG	0.582279	7.4:+39161429	MS.gene055131:CDS
CAATAGAATGATGTGTGGAT+CGG	0.613976	7.4:-39161323	None:intergenic
AGGAACACCATTTGCAGTGG+TGG	0.618142	7.4:-39161258	None:intergenic
GCTGTTATGTCGTTTAATGG+TGG	0.619102	7.4:+39161569	MS.gene055131:CDS
GTCGCCACCGTGGAGTTGGA+AGG	0.621088	7.4:-39161278	None:intergenic
ACATTGTTAAACTCAAACCT+GGG	0.621137	7.4:+39161465	MS.gene055131:CDS
TGGTGTTCCTTCCAACTCCA+CGG	0.621560	7.4:+39161271	MS.gene055131:CDS
GGGACATCAAACGACGTCGT+TGG	0.623966	7.4:+39161485	MS.gene055131:CDS
GGAAGGAACACCATTTGCAG+TGG	0.632915	7.4:-39161261	None:intergenic
ACTATTCCATTGCTGGCCCG+GGG	0.640157	7.4:+39161364	MS.gene055131:CDS
ATCCACACATCATTCTATTG+GGG	0.654547	7.4:+39161326	MS.gene055131:CDS
AAACCATAAACCCAATAACA+TGG	0.667055	7.4:-39161616	None:intergenic
CAGCGCTGTCATGTACGCGG+TGG	0.674127	7.4:+39161388	MS.gene055131:CDS
GACCCCAATAGAATGATGTG+TGG	0.683603	7.4:-39161328	None:intergenic
GCCCAACATCGTCGCCACCG+TGG	0.693359	7.4:-39161288	None:intergenic
CCAAGTACGAGAATCCAGTA+CGG	0.693631	7.4:-39161538	None:intergenic
GGACAGCGCTGTCATGTACG+CGG	0.705704	7.4:+39161385	MS.gene055131:CDS
ACACCGACATTACAAACACG+AGG	0.732297	7.4:-39161418	None:intergenic
TGTTCCTTCCAACTCCACGG+TGG	0.776714	7.4:+39161274	MS.gene055131:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAACCATAAACCCAATAACA+TGG	-	chr7.4:39161619-39161638	None:intergenic	30.0%
	AACATTGTTAAACTCAAACC+TGG	+	chr7.4:39161464-39161483	MS.gene055131:CDS	30.0%
	ACATTGTTAAACTCAAACCT+GGG	+	chr7.4:39161465-39161484	MS.gene055131:CDS	30.0%
!	AGGTTTGAGTTTAACAATGT+TGG	-	chr7.4:39161465-39161484	None:intergenic	30.0%
!	GTTATTGGGTTTATGGTTTT+TGG	+	chr7.4:39161620-39161639	MS.gene055131:CDS	30.0%
!!	ATGGCTATTTTGAAGAAATC+TGG	+	chr7.4:39161647-39161666	MS.gene055131:CDS	30.0%
!!	CTATTTTGAAGAAATCTGGT+GGG	+	chr7.4:39161651-39161670	MS.gene055131:CDS	30.0%
	ATCCACACATCATTCTATTG+GGG	+	chr7.4:39161326-39161345	MS.gene055131:CDS	35.0%
	ATTCTCGTACTTGGTTATGT+TGG	+	chr7.4:39161547-39161566	MS.gene055131:CDS	35.0%
	CAATAGAATGATGTGTGGAT+CGG	-	chr7.4:39161326-39161345	None:intergenic	35.0%
	GATCCACACATCATTCTATT+GGG	+	chr7.4:39161325-39161344	MS.gene055131:CDS	35.0%
	TAGACATACTATTCCATTGC+TGG	+	chr7.4:39161357-39161376	MS.gene055131:CDS	35.0%
!	TTGGCTGTTATGTCGTTTAA+TGG	+	chr7.4:39161566-39161585	MS.gene055131:CDS	35.0%
!!	GCTATTTTGAAGAAATCTGG+TGG	+	chr7.4:39161650-39161669	MS.gene055131:CDS	35.0%
!!!	GTTTATGGTTTTTGGTACGA+TGG	+	chr7.4:39161628-39161647	MS.gene055131:CDS	35.0%
	CGATCCACACATCATTCTAT+TGG	+	chr7.4:39161324-39161343	MS.gene055131:CDS	40.0%
	GATCCTCGTGTTTGTAATGT+CGG	+	chr7.4:39161415-39161434	MS.gene055131:CDS	40.0%
	TGGCCATGTTATTGGGTTTA+TGG	+	chr7.4:39161613-39161632	MS.gene055131:CDS	40.0%
!	GCTGTTATGTCGTTTAATGG+TGG	+	chr7.4:39161569-39161588	MS.gene055131:CDS	40.0%
!!	GTTGGCTCACTTTTAAGAAC+GGG	+	chr7.4:39161503-39161522	MS.gene055131:CDS	40.0%
!!	TTGGCTCACTTTTAAGAACG+GGG	+	chr7.4:39161504-39161523	MS.gene055131:CDS	40.0%
!!!	CGTTGGCTCACTTTTAAGAA+CGG	+	chr7.4:39161502-39161521	MS.gene055131:CDS	40.0%
	ACACCGACATTACAAACACG+AGG	-	chr7.4:39161421-39161440	None:intergenic	45.0%
	ATACTATTCCATTGCTGGCC+CGG	+	chr7.4:39161362-39161381	MS.gene055131:CDS	45.0%
	CCAAGTACGAGAATCCAGTA+CGG	-	chr7.4:39161541-39161560	None:intergenic	45.0%
	CGTGTTTGTAATGTCGGTGT+TGG	+	chr7.4:39161421-39161440	MS.gene055131:CDS	45.0%
	GACCCCAATAGAATGATGTG+TGG	-	chr7.4:39161331-39161350	None:intergenic	45.0%
	TTGTTCACCACCACTGCAAA+TGG	+	chr7.4:39161251-39161270	MS.gene055131:CDS	45.0%
!!	CAGTTGGTGGCCATGTTATT+GGG	+	chr7.4:39161606-39161625	MS.gene055131:CDS	45.0%
!!	CTTTTAAGAACGGGGCTGTA+CGG	+	chr7.4:39161512-39161531	MS.gene055131:CDS	45.0%
!!	GTTTGTAATGTCGGTGTTGG+TGG	+	chr7.4:39161424-39161443	MS.gene055131:CDS	45.0%
	AGGAACACCATTTGCAGTGG+TGG	-	chr7.4:39161261-39161280	None:intergenic	50.0%
	CCGTACTGGATTCTCGTACT+TGG	+	chr7.4:39161538-39161557	MS.gene055131:CDS	50.0%
	GGAAGGAACACCATTTGCAG+TGG	-	chr7.4:39161264-39161283	None:intergenic	50.0%
	GGTGTGTTTCTTGCTGCAGT+TGG	+	chr7.4:39161590-39161609	MS.gene055131:CDS	50.0%
	GTGTTTCTTGCTGCAGTTGG+TGG	+	chr7.4:39161593-39161612	MS.gene055131:CDS	50.0%
	TACTATTCCATTGCTGGCCC+GGG	+	chr7.4:39161363-39161382	MS.gene055131:CDS	50.0%
	TGGTGTTCCTTCCAACTCCA+CGG	+	chr7.4:39161271-39161290	MS.gene055131:CDS	50.0%
	TGTGGATCGGAATTCTTCGC+CGG	-	chr7.4:39161313-39161332	None:intergenic	50.0%
!!	GCAGTTGGTGGCCATGTTAT+TGG	+	chr7.4:39161605-39161624	MS.gene055131:CDS	50.0%
!!	TAATGTCGGTGTTGGTGGAG+TGG	+	chr7.4:39161429-39161448	MS.gene055131:CDS	50.0%
	ACGACGTCGTTTGATGTCCC+AGG	-	chr7.4:39161485-39161504	None:intergenic	55.0%
	ACTATTCCATTGCTGGCCCG+GGG	+	chr7.4:39161364-39161383	MS.gene055131:CDS	55.0%
	GGGACATCAAACGACGTCGT+TGG	+	chr7.4:39161485-39161504	MS.gene055131:CDS	55.0%
	TGTTCCTTCCAACTCCACGG+TGG	+	chr7.4:39161274-39161293	MS.gene055131:CDS	55.0%
	GGACAGCGCTGTCATGTACG+CGG	+	chr7.4:39161385-39161404	MS.gene055131:CDS	60.0%
	GTACATGACAGCGCTGTCCC+CGG	-	chr7.4:39161384-39161403	None:intergenic	60.0%
	TACATGACAGCGCTGTCCCC+GGG	-	chr7.4:39161383-39161402	None:intergenic	60.0%
	TCCACGGTGGCGACGATGTT+GGG	+	chr7.4:39161287-39161306	MS.gene055131:CDS	60.0%
	CAGCGCTGTCATGTACGCGG+TGG	+	chr7.4:39161388-39161407	MS.gene055131:CDS	65.0%
	CATCGTCGCCACCGTGGAGT+TGG	-	chr7.4:39161285-39161304	None:intergenic	65.0%
	CTCCACGGTGGCGACGATGT+TGG	+	chr7.4:39161286-39161305	MS.gene055131:CDS	65.0%
	GGGCTGTACGGTGTCCGTAC+TGG	+	chr7.4:39161524-39161543	MS.gene055131:CDS	65.0%
	GTCGCCACCGTGGAGTTGGA+AGG	-	chr7.4:39161281-39161300	None:intergenic	65.0%
	CGGTGGCGACGATGTTGGGC+CGG	+	chr7.4:39161291-39161310	MS.gene055131:CDS	70.0%
	GCCCAACATCGTCGCCACCG+TGG	-	chr7.4:39161291-39161310	None:intergenic	70.0%
	CGCTGTCCCCGGGCCAGCAA+TGG	-	chr7.4:39161373-39161392	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	39161248	39161697	39161248	ID=MS.gene055131
chr7.4	mRNA	39161248	39161697	39161248	ID=MS.gene055131.t1;Parent=MS.gene055131
chr7.4	exon	39161248	39161697	39161248	ID=MS.gene055131.t1.exon1;Parent=MS.gene055131.t1
chr7.4	CDS	39161248	39161697	39161248	ID=cds.MS.gene055131.t1;Parent=MS.gene055131.t1

Gene Sequence

>MS.gene055131

ATGTTGTTCACCACCACTGCAAATGGTGTTCCTTCCAACTCCACGGTGGCGACGATGTTGGGCCGGCGAAGAATTCCGATCCACACATCATTCTATTGGGGTCATAATGTAGACATACTATTCCATTGCTGGCCCGGGGACAGCGCTGTCATGTACGCGGTGGCGCTGATCCTCGTGTTTGTAATGTCGGTGTTGGTGGAGTGGCTTTCATTTACCAACATTGTTAAACTCAAACCTGGGACATCAAACGACGTCGTTGGCTCACTTTTAAGAACGGGGCTGTACGGTGTCCGTACTGGATTCTCGTACTTGGTTATGTTGGCTGTTATGTCGTTTAATGGTGGTGTGTTTCTTGCTGCAGTTGGTGGCCATGTTATTGGGTTTATGGTTTTTGGTACGATGGCTATTTTGAAGAAATCTGGTGGGTTAGATTCTTCTTCTAAACCATAG

Protein sequence

>MS.gene055131.t1

MLFTTTANGVPSNSTVATMLGRRRIPIHTSFYWGHNVDILFHCWPGDSAVMYAVALILVFVMSVLVEWLSFTNIVKLKPGTSNDVVGSLLRTGLYGVRTGFSYLVMLAVMSFNGGVFLAAVGGHVIGFMVFGTMAILKKSGGLDSSSKP