AlfalfaGEDB | MS.gene95848

M. sativa cultivar XinJiangDaYe / MS.gene95848

KEGG: K11096
GO: GO:0005575 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0016070 GO:0030532 GO:0032991 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:0120114 GO:1901360 GO:1990904
PFAM: PF01423.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95848.t1	XP_003630987.1	100	108	0	0	1	108	1	108	7.30E-54	219.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95848.t1	Q3SZF8	84.5	103	16	0	5	107	11	113	2.4e-45	182.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95848.t1	G7LBH6	100.0	108	0	0	1	108	1	108	5.3e-54	219.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055522	MS.gene95848	0.844347	8.00E-59	-1.69E-46
MS.gene057641	MS.gene95848	0.811643	6.32E-51	-1.69E-46
MS.gene057643	MS.gene95848	0.826409	2.76E-54	-1.69E-46
MS.gene060483	MS.gene95848	0.825306	5.04E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene82776	MS.gene95848	PPI
MS.gene43700	MS.gene95848	PPI
MS.gene058567	MS.gene95848	PPI
MS.gene95848	MS.gene009798	PPI
MS.gene95848	MS.gene97334	PPI
MS.gene95848	MS.gene62660	PPI
MS.gene065862	MS.gene95848	PPI
MS.gene24692	MS.gene95848	PPI
MS.gene053099	MS.gene95848	PPI
MS.gene045470	MS.gene95848	PPI
MS.gene95848	MS.gene56983	PPI
MS.gene95848	MS.gene90813	PPI
MS.gene95848	MS.gene48652	PPI
MS.gene95848	MS.gene029082	PPI
MS.gene95848	MS.gene015624	PPI
MS.gene95848	MS.gene065853	PPI
MS.gene95848	MS.gene35064	PPI
MS.gene95848	MS.gene032681	PPI
MS.gene95848	MS.gene64804	PPI
MS.gene95848	MS.gene055162	PPI
MS.gene95848	MS.gene035486	PPI
MS.gene95848	MS.gene70292	PPI
MS.gene028868	MS.gene95848	PPI
MS.gene051241	MS.gene95848	PPI
MS.gene95848	MS.gene065836	PPI
MS.gene95848	MS.gene073888	PPI
MS.gene95848	MS.gene055778	PPI
MS.gene50211	MS.gene95848	PPI
MS.gene95848	MS.gene053443	PPI
MS.gene95848	MS.gene030697	PPI
MS.gene068867	MS.gene95848	PPI
MS.gene95848	MS.gene035725	PPI
MS.gene95848	MS.gene58148	PPI
MS.gene95848	MS.gene028322	PPI
MS.gene95848	MS.gene92231	PPI
MS.gene95848	MS.gene67483	PPI
MS.gene95848	MS.gene028393	PPI
MS.gene95848	MS.gene36469	PPI
MS.gene95848	MS.gene012386	PPI
MS.gene95848	MS.gene065857	PPI
MS.gene95848	MS.gene043867	PPI
MS.gene59503	MS.gene95848	PPI
MS.gene95848	MS.gene051172	PPI
MS.gene95848	MS.gene83565	PPI
MS.gene070248	MS.gene95848	PPI
MS.gene95848	MS.gene35136	PPI
MS.gene95848	MS.gene93333	PPI
MS.gene95848	MS.gene070731	PPI
MS.gene95848	MS.gene040757	PPI
MS.gene95848	MS.gene051245	PPI
MS.gene83322	MS.gene95848	PPI
MS.gene95848	MS.gene069779	PPI
MS.gene95848	MS.gene23567	PPI
MS.gene95848	MS.gene86968	PPI
MS.gene069779	MS.gene95848	PPI
MS.gene95848	MS.gene043455	PPI
MS.gene95848	MS.gene28762	PPI
MS.gene95848	MS.gene073734	PPI
MS.gene95848	MS.gene31434	PPI
MS.gene95848	MS.gene28172	PPI
MS.gene44813	MS.gene95848	PPI
MS.gene95848	MS.gene062632	PPI
MS.gene95848	MS.gene058567	PPI
MS.gene95848	MS.gene067444	PPI
MS.gene006712	MS.gene95848	PPI
MS.gene95848	MS.gene007200	PPI
MS.gene95848	MS.gene050303	PPI
MS.gene95848	MS.gene060774	PPI
MS.gene046119	MS.gene95848	PPI
MS.gene95848	MS.gene85931	PPI
MS.gene53517	MS.gene95848	PPI
MS.gene95848	MS.gene023131	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95848.t1	MTR_8g105890	100.000	108	0	0	1	108	1	108	2.20e-76	221
MS.gene95848.t1	MTR_8g105890	100.000	100	0	0	9	108	25	124	6.28e-70	205
MS.gene95848.t1	MTR_5g097030	91.509	106	8	1	3	108	55	159	4.52e-65	194
MS.gene95848.t1	MTR_8g105890	100.000	82	0	0	27	108	1	82	5.79e-56	168

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95848.t1	AT3G62840	93.519	108	7	0	1	108	1	108	3.43e-71	207
MS.gene95848.t1	AT2G47640	93.519	108	7	0	1	108	1	108	3.43e-71	207
MS.gene95848.t1	AT2G47640	93.519	108	7	0	1	108	1	108	3.43e-71	207
MS.gene95848.t1	AT3G62840	92.661	109	7	1	1	108	1	109	1.35e-69	203
MS.gene95848.t1	AT2G47640	92.661	109	7	1	1	108	1	109	1.35e-69	203
MS.gene95848.t1	AT2G47640	92.661	109	7	1	1	108	1	109	1.35e-69	203

Find 29 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATGGAGATACTAGCTTATT+TGG	0.120204	4.3:-1167663	None:intergenic
ATTCTGTCATCATTGTTCTT+AGG	0.279548	4.3:+1167636	MS.gene95848:CDS
ATGGAGATACTAGCTTATTT+GGG	0.292207	4.3:-1167662	None:intergenic
AAAAGCTTCTAGGCCGTGTC+AGG	0.374760	4.3:+1167372	MS.gene95848:CDS
TTCTTTCCTTTACCAGTCTT+AGG	0.400785	4.3:-1167557	None:intergenic
ATACCTCAGTCCACATTTCC+CGG	0.427349	4.3:-1167433	None:intergenic
ATATGGTTCTTGAAAATGTC+CGG	0.429724	4.3:+1167414	MS.gene95848:CDS
CTCAATTGTTACAGGCTGTC+GGG	0.432771	4.3:+1167157	MS.gene95848:intron
ATCAGCAAAATGTTCCTTCG+TGG	0.434985	4.3:+1167611	MS.gene95848:CDS
TAGCTTACATCTTCTTCCAT+TGG	0.480536	4.3:-1166502	None:intergenic
CAGGTGCCTAAGACTGGTAA+AGG	0.497162	4.3:+1167551	MS.gene95848:intron
TTACATCTTCTTCCATTGGC+CGG	0.497216	4.3:-1166498	None:intergenic
TTGAAAATGTCCGGGAAATG+TGG	0.500328	4.3:+1167423	MS.gene95848:CDS
CTCATCATGAGTACAGATAG+TGG	0.509132	4.3:-1167210	None:intergenic
ATGTATCAGGTGCCTAAGAC+TGG	0.510734	4.3:+1167545	MS.gene95848:intron
GAAGGCTCAGCCAGTCAACA+AGG	0.537512	4.3:+1167580	MS.gene95848:CDS
ATGAAACGATCCTTGTTGAC+TGG	0.541044	4.3:-1167590	None:intergenic
ACTCAATTGTTACAGGCTGT+CGG	0.551091	4.3:+1167156	MS.gene95848:intron
TTCTTCTTGAAGCCGGCCAA+TGG	0.551208	4.3:+1166486	MS.gene95848:intron
GACTGGTAAAGGAAAGAAGA+AGG	0.552162	4.3:+1167562	MS.gene95848:CDS
TGTCGGGAAGAATGAGGAAG+AGG	0.555164	4.3:+1167173	MS.gene95848:CDS
TATGGTTCTTGAAAATGTCC+GGG	0.564509	4.3:+1167415	MS.gene95848:CDS
GTCTATCAAAAGCCCTGACA+CGG	0.582472	4.3:-1167385	None:intergenic
TGGAGATACTAGCTTATTTG+GGG	0.598626	4.3:-1167661	None:intergenic
ACAGGCTGTCGGGAAGAATG+AGG	0.617342	4.3:+1167167	MS.gene95848:intron
GAGGAAGAGGAATTCAACAC+TGG	0.639973	4.3:+1167186	MS.gene95848:CDS
AAAGCTTCTAGGCCGTGTCA+GGG	0.669612	4.3:+1167373	MS.gene95848:CDS
TGTCCGGGAAATGTGGACTG+AGG	0.699661	4.3:+1167430	MS.gene95848:CDS
TGATGACAGAATCTCCACGA+AGG	0.746510	4.3:-1167625	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTTTTTAAAAAACAG+AGG	+	chr4.3:1167011-1167030	MS.gene95848:intron	10.0%
!!!	ACTTTGACTTTAATGTTAAA+TGG	+	chr4.3:1167089-1167108	MS.gene95848:intron	20.0%
!!!	TGTTTTATCATTACATAACT+TGG	+	chr4.3:1166800-1166819	MS.gene95848:intron	20.0%
!	ATCATTACATAACTTGGTAA+AGG	+	chr4.3:1166806-1166825	MS.gene95848:intron	25.0%
!	TGTATCTTCTGTTAAAATCA+AGG	-	chr4.3:1166654-1166673	None:intergenic	25.0%
!!	TCAATATGCTCTATTTGTTT+TGG	+	chr4.3:1166747-1166766	MS.gene95848:intron	25.0%
!!!	ATTTGAATTTACTTTTCTGC+TGG	+	chr4.3:1167496-1167515	MS.gene95848:intron	25.0%
!!!	ATTTTAACAGAAGATACACT+TGG	+	chr4.3:1166656-1166675	MS.gene95848:intron	25.0%
	ATATGGTTCTTGAAAATGTC+CGG	+	chr4.3:1167414-1167433	MS.gene95848:CDS	30.0%
	ATGCAAATTACTTCTCTACT+TGG	+	chr4.3:1167470-1167489	MS.gene95848:intron	30.0%
	ATTCTGTCATCATTGTTCTT+AGG	+	chr4.3:1167636-1167655	MS.gene95848:CDS	30.0%
	GAAGAAAGAAAAAAGAAGAC+TGG	-	chr4.3:1167045-1167064	None:intergenic	30.0%
	TGAAATCACAATTCTGAATC+AGG	-	chr4.3:1166773-1166792	None:intergenic	30.0%
	TGTTCTATTTAGTGCACATT+TGG	+	chr4.3:1166595-1166614	MS.gene95848:intron	30.0%
	TTTGCATTATGATCCTGATA+TGG	+	chr4.3:1166557-1166576	MS.gene95848:intron	30.0%
!	GATTTTACAAACACTTATGC+AGG	+	chr4.3:1167325-1167344	MS.gene95848:intron	30.0%
!	TTGATTTTTCTTCTTGAAGC+CGG	+	chr4.3:1166479-1166498	MS.gene95848:intron	30.0%
!	TTTTGATAGACACTGCAATA+TGG	+	chr4.3:1167397-1167416	MS.gene95848:CDS	30.0%
	AAAAAAGAAGACTGGACTTC+AGG	-	chr4.3:1167037-1167056	None:intergenic	35.0%
	ACAGATATGCAATCCATATC+AGG	-	chr4.3:1166573-1166592	None:intergenic	35.0%
	ATGTTCTGCTTGTATGTATC+AGG	+	chr4.3:1167532-1167551	MS.gene95848:intron	35.0%
	ATTTGGATTGACCTTGAGTT+TGG	+	chr4.3:1166612-1166631	MS.gene95848:intron	35.0%
	CGTAACAACAAAAAGCTTCT+AGG	+	chr4.3:1167362-1167381	MS.gene95848:CDS	35.0%
	CTGACATACTCAATTGTTAC+AGG	+	chr4.3:1167149-1167168	MS.gene95848:intron	35.0%
	TACTGTGTGTTCTTCTTGAT+CGG	+	chr4.3:1167280-1167299	MS.gene95848:intron	35.0%
	TAGCTTACATCTTCTTCCAT+TGG	-	chr4.3:1166505-1166524	None:intergenic	35.0%
	TATGGTTCTTGAAAATGTCC+GGG	+	chr4.3:1167415-1167434	MS.gene95848:CDS	35.0%
	TGAGTTTGACTAAATCATGC+TGG	-	chr4.3:1166703-1166722	None:intergenic	35.0%
	TTCTTTCCTTTACCAGTCTT+AGG	-	chr4.3:1167560-1167579	None:intergenic	35.0%
!!	TGAATTTACTTTTCTGCTGG+TGG	+	chr4.3:1167499-1167518	MS.gene95848:intron	35.0%
	ACTCAATTGTTACAGGCTGT+CGG	+	chr4.3:1167156-1167175	MS.gene95848:intron	40.0%
	ATCAGCAAAATGTTCCTTCG+TGG	+	chr4.3:1167611-1167630	MS.gene95848:CDS	40.0%
	ATGAAACGATCCTTGTTGAC+TGG	-	chr4.3:1167593-1167612	None:intergenic	40.0%
	CTCATCATGAGTACAGATAG+TGG	-	chr4.3:1167213-1167232	None:intergenic	40.0%
	GACTGGTAAAGGAAAGAAGA+AGG	+	chr4.3:1167562-1167581	MS.gene95848:CDS	40.0%
	GAGTGTGAAAAACAACACAC+AGG	+	chr4.3:1167230-1167249	MS.gene95848:CDS	40.0%
	TCTTTCTTCATGCGTCACAA+TGG	+	chr4.3:1167056-1167075	MS.gene95848:intron	40.0%
	TGAGGTATGTATTCTCTTGC+TGG	+	chr4.3:1166384-1166403	MS.gene95848:intron	40.0%
	TTACATCTTCTTCCATTGGC+CGG	-	chr4.3:1166501-1166520	None:intergenic	40.0%
	TTGAAAATGTCCGGGAAATG+TGG	+	chr4.3:1167423-1167442	MS.gene95848:CDS	40.0%
!	TGAAAGTCAGCTTTGTGTTG+TGG	+	chr4.3:1166893-1166912	MS.gene95848:intron	40.0%
	ATACCTCAGTCCACATTTCC+CGG	-	chr4.3:1167436-1167455	None:intergenic	45.0%
	ATGTATCAGGTGCCTAAGAC+TGG	+	chr4.3:1167545-1167564	MS.gene95848:intron	45.0%
	CTCAATTGTTACAGGCTGTC+GGG	+	chr4.3:1167157-1167176	MS.gene95848:intron	45.0%
	GAGGAAGAGGAATTCAACAC+TGG	+	chr4.3:1167186-1167205	MS.gene95848:CDS	45.0%
	GTCTATCAAAAGCCCTGACA+CGG	-	chr4.3:1167388-1167407	None:intergenic	45.0%
	TGATGACAGAATCTCCACGA+AGG	-	chr4.3:1167628-1167647	None:intergenic	45.0%
	TGGATTGACCTTGAGTTTGG+CGG	+	chr4.3:1166615-1166634	MS.gene95848:intron	45.0%
!!	AAAAAAAAAAAAAAAAAAGA+TGG	-	chr4.3:1166997-1167016	None:intergenic	5.0%
	AAAAGCTTCTAGGCCGTGTC+AGG	+	chr4.3:1167372-1167391	MS.gene95848:CDS	50.0%
	AAAGCTTCTAGGCCGTGTCA+GGG	+	chr4.3:1167373-1167392	MS.gene95848:CDS	50.0%
	CAGGTGCCTAAGACTGGTAA+AGG	+	chr4.3:1167551-1167570	MS.gene95848:intron	50.0%
	CCAAGATTCCGCCAAACTCA+AGG	-	chr4.3:1166626-1166645	None:intergenic	50.0%
	CCTTGAGTTTGGCGGAATCT+TGG	+	chr4.3:1166623-1166642	MS.gene95848:intron	50.0%
	TGTCGGGAAGAATGAGGAAG+AGG	+	chr4.3:1167173-1167192	MS.gene95848:CDS	50.0%
	TTCTTCTTGAAGCCGGCCAA+TGG	+	chr4.3:1166486-1166505	MS.gene95848:intron	50.0%
	ACAGGCTGTCGGGAAGAATG+AGG	+	chr4.3:1167167-1167186	MS.gene95848:intron	55.0%
	GAAGGCTCAGCCAGTCAACA+AGG	+	chr4.3:1167580-1167599	MS.gene95848:CDS	55.0%
!	TGTCCGGGAAATGTGGACTG+AGG	+	chr4.3:1167430-1167449	MS.gene95848:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	1166383	1167670	1166383	ID=MS.gene95848
chr4.3	mRNA	1166383	1167670	1166383	ID=MS.gene95848.t1;Parent=MS.gene95848
chr4.3	exon	1166383	1166387	1166383	ID=MS.gene95848.t1.exon1;Parent=MS.gene95848.t1
chr4.3	CDS	1166383	1166387	1166383	ID=cds.MS.gene95848.t1;Parent=MS.gene95848.t1
chr4.3	exon	1166498	1166516	1166498	ID=MS.gene95848.t1.exon2;Parent=MS.gene95848.t1
chr4.3	CDS	1166498	1166516	1166498	ID=cds.MS.gene95848.t1;Parent=MS.gene95848.t1
chr4.3	exon	1167171	1167251	1167171	ID=MS.gene95848.t1.exon3;Parent=MS.gene95848.t1
chr4.3	CDS	1167171	1167251	1167171	ID=cds.MS.gene95848.t1;Parent=MS.gene95848.t1
chr4.3	exon	1167347	1167451	1167347	ID=MS.gene95848.t1.exon4;Parent=MS.gene95848.t1
chr4.3	CDS	1167347	1167451	1167347	ID=cds.MS.gene95848.t1;Parent=MS.gene95848.t1
chr4.3	exon	1167554	1167670	1167554	ID=MS.gene95848.t1.exon5;Parent=MS.gene95848.t1
chr4.3	CDS	1167554	1167670	1167554	ID=cds.MS.gene95848.t1;Parent=MS.gene95848.t1

Gene Sequence

>MS.gene95848

ATGAGGTATGTATTCTCTTGCTGGTTATTTTGTGAAATTGAGATATATTATTATCATTATTTTTAAATTTTCAGTTGTCTAACATTTTTTGTTTTCTTGATTTTTCTTCTTGAAGCCGGCCAATGGAAGAAGATGTAAGCTACTTATTCTTTAATGATATTTTTTATCACAAGTTTTGCATTATGATCCTGATATGGATTGCATATCTGTTATGTTCTATTTAGTGCACATTTGGATTGACCTTGAGTTTGGCGGAATCTTGGTGTCACCTTGATTTTAACAGAAGATACACTTGGAGTTTCAACAATATTGCAGTGCCAGCATGATTTAGTCAAACTCACAGTCAAGCTAAACATTTAAGTACTCAATATGCTCTATTTGTTTTGGCCTGATTCAGAATTGTGATTTCATCATATCTGTTTTATCATTACATAACTTGGTAAAGGATTTTTAGTTTATGATGATTTCAAAATTTAGTTTATGTTGCTAAAAAAAATGTTCTGTTAATTTTGAAAGTCAGCTTTGTGTTGTGGCTATGTTTCTTAAAAATGAAATACTAATTTGTTTCTACATTTAGTAACACTACTTTTCTTTATTGAATTTAAGTTTTGCCATCTTTTTTTTTTTTTTTTTTTTTTTAAAAAACAGAGGCCTGAAGTCCAGTCTTCTTTTTTCTTTCTTCATGCGTCACAATGGTTCTTACTGCACTTTGACTTTAATGTTAAATGGCTGTTTAAAGTTTAATATGCTTTATAATCTTGTCAAGCTGACATACTCAATTGTTACAGGCTGTCGGGAAGAATGAGGAAGAGGAATTCAACACTGGTCCACTATCTGTACTCATGATGAGTGTGAAAAACAACACACAGGTATTTTTTTTATACGACACACTCATAGTACTGTGTGTTCTTCTTGATCGGTGCTCAGAATGTTTTTGATGCTGATTTTACAAACACTTATGCAGGTTCTGATCAATTGTCGTAACAACAAAAAGCTTCTAGGCCGTGTCAGGGCTTTTGATAGACACTGCAATATGGTTCTTGAAAATGTCCGGGAAATGTGGACTGAGGTATGCGACATTTTGTTTATGCAAATTACTTCTCTACTTGGTACATTTGAATTTACTTTTCTGCTGGTGGCATATGCTAAATGTTCTGCTTGTATGTATCAGGTGCCTAAGACTGGTAAAGGAAAGAAGAAGGCTCAGCCAGTCAACAAGGATCGTTTCATCAGCAAAATGTTCCTTCGTGGAGATTCTGTCATCATTGTTCTTAGGAACCCCAAATAA

Protein sequence

>MS.gene95848.t1

MSRPMEEDAVGKNEEEEFNTGPLSVLMMSVKNNTQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKAQPVNKDRFISKMFLRGDSVIIVLRNPK