Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055548.t1 | XP_003602750.2 | 96.6 | 149 | 4 | 1 | 1 | 149 | 6 | 153 | 6.30E-72 | 280 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055548.t1 | A0A072V1G3 | 96.6 | 149 | 4 | 1 | 1 | 149 | 6 | 153 | 4.5e-72 | 280.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049162 | MS.gene055548 | 0.947607 | 4.84E-106 | -1.69E-46 |
| MS.gene055547 | MS.gene055548 | 0.96088 | 4.65E-119 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055548.t1 | MTR_3g098620 | 96.644 | 149 | 4 | 1 | 1 | 149 | 6 | 153 | 2.96e-93 | 289 |
| MS.gene055548.t1 | MTR_3g098620 | 96.644 | 149 | 4 | 1 | 1 | 149 | 6 | 153 | 3.08e-93 | 289 |
| MS.gene055548.t1 | MTR_3g098620 | 96.644 | 149 | 4 | 1 | 1 | 149 | 6 | 153 | 6.02e-93 | 289 |
| MS.gene055548.t1 | MTR_8g100160 | 55.333 | 150 | 63 | 3 | 1 | 149 | 6 | 152 | 1.01e-44 | 156 |
| MS.gene055548.t1 | MTR_8g100160 | 55.333 | 150 | 63 | 3 | 1 | 149 | 6 | 152 | 1.02e-44 | 156 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAGCTCATATGGTTTAAT+TGG | 0.114750 | 3.2:+81825338 | MS.gene055548:CDS |
| TAATCATCATGAATAGATTT+TGG | 0.178888 | 3.2:-81824396 | None:intergenic |
| GAAACACATTCTTACACTTT+TGG | 0.261999 | 3.2:-81825433 | None:intergenic |
| TGCAGAGGAATGTTGGATTA+AGG | 0.349422 | 3.2:+81824370 | MS.gene055548:intron |
| CTAGCTCATATGGTTTAATT+GGG | 0.413117 | 3.2:+81825339 | MS.gene055548:CDS |
| CAGCCAGCATCTAGCTCATA+TGG | 0.414663 | 3.2:+81825329 | MS.gene055548:CDS |
| AACAAGATTGTCACCAATGC+AGG | 0.425656 | 3.2:+81825362 | MS.gene055548:CDS |
| AGGAAACATCATCATAAAGA+TGG | 0.434351 | 3.2:-81824171 | None:intergenic |
| ATGTCACAGACTAGTTTATC+CGG | 0.437906 | 3.2:+81825194 | MS.gene055548:CDS |
| GCTTTCACGTGCGCCTGCAT+TGG | 0.451678 | 3.2:-81825375 | None:intergenic |
| TGAATTATCACCCGAGATCA+TGG | 0.457507 | 3.2:+81825291 | MS.gene055548:CDS |
| GATAAACTAGTCTGTGACAT+TGG | 0.480263 | 3.2:-81825191 | None:intergenic |
| TTTCATGTGCAGAGGAATGT+TGG | 0.480325 | 3.2:+81824363 | MS.gene055548:intron |
| AGTGAAATGGCAGCTTCACC+TGG | 0.484170 | 3.2:+81825116 | MS.gene055548:CDS |
| TGCACATCAGACCATGATCT+CGG | 0.501021 | 3.2:-81825302 | None:intergenic |
| AATAATTACCTCAGAAGCAT+AGG | 0.505187 | 3.2:-81824191 | None:intergenic |
| AAACCATATGAGCTAGATGC+TGG | 0.540798 | 3.2:-81825332 | None:intergenic |
| GGAAACATCATCATAAAGAT+GGG | 0.545230 | 3.2:-81824170 | None:intergenic |
| TGTCACAGACTAGTTTATCC+GGG | 0.553964 | 3.2:+81825195 | MS.gene055548:CDS |
| AAGCAGTAGTAACACCCCTC+TGG | 0.563767 | 3.2:-81824144 | None:intergenic |
| GCACATCAGACCATGATCTC+GGG | 0.565376 | 3.2:-81825301 | None:intergenic |
| TGGCATGGTAGATGCGCTGA+AGG | 0.585812 | 3.2:+81825253 | MS.gene055548:CDS |
| AGATGCGCTGAAGGATTCAA+AGG | 0.602052 | 3.2:+81825262 | MS.gene055548:CDS |
| GATCATGGTCTGATGTGCAT+AGG | 0.608179 | 3.2:+81825306 | MS.gene055548:CDS |
| TGATGTTTCCTATGCTTCTG+AGG | 0.609927 | 3.2:+81824183 | MS.gene055548:CDS |
| GAAGTGTGCAAAATGCCTCC+AGG | 0.633750 | 3.2:-81825134 | None:intergenic |
| ATTCATGATGATTATCCACA+AGG | 0.660419 | 3.2:+81824405 | MS.gene055548:CDS |
| GTCACAGACTAGTTTATCCG+GGG | 0.680067 | 3.2:+81825196 | MS.gene055548:CDS |
| GAAATGGCAGCTTCACCTGG+AGG | 0.683059 | 3.2:+81825119 | MS.gene055548:CDS |
| TTTGAAATAATGAACCAGAG+GGG | 0.758778 | 3.2:+81824130 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGACATGTTTAGAAAAAAAA+AGG | - | chr3.2:81824337-81824356 | None:intergenic | 20.0% |
| !! | TAAATAAGCAGTTCATTTAA+TGG | + | chr3.2:81825038-81825057 | MS.gene055548:intron | 20.0% |
| !! | TTAAATGAACTGCTTATTTA+TGG | - | chr3.2:81825038-81825057 | None:intergenic | 20.0% |
| !! | TTAGAATAAATAACTATGGA+TGG | + | chr3.2:81824750-81824769 | MS.gene055548:intron | 20.0% |
| !!! | ATCGTTTATTTCATTTTGTA+GGG | + | chr3.2:81824784-81824803 | MS.gene055548:intron | 20.0% |
| !!! | GCATTTAGAATAAATAACTA+TGG | + | chr3.2:81824746-81824765 | MS.gene055548:intron | 20.0% |
| !!! | GGTATTAATAGATTTCTTTT+TGG | + | chr3.2:81824810-81824829 | MS.gene055548:intron | 20.0% |
| !!! | GTTTTTAATCATGATGATAT+TGG | + | chr3.2:81824955-81824974 | MS.gene055548:intron | 20.0% |
| !!! | TAATCATCATGAATAGATTT+TGG | - | chr3.2:81824399-81824418 | None:intergenic | 20.0% |
| !!! | TTTTTTTTCTAAACATGTCT+TGG | + | chr3.2:81824337-81824356 | MS.gene055548:intron | 20.0% |
| ! | AAAAAAAGGCAATAACATTG+AGG | - | chr3.2:81824323-81824342 | None:intergenic | 25.0% |
| ! | AAAACCAAAATGAGACAATT+CGG | - | chr3.2:81824219-81824238 | None:intergenic | 25.0% |
| ! | ATAAAAAACACTCAAAGTCT+TGG | - | chr3.2:81824260-81824279 | None:intergenic | 25.0% |
| ! | CTTTGGTTAGTAGATATATA+TGG | - | chr3.2:81824506-81824525 | None:intergenic | 25.0% |
| !! | AGTTCTATGTTCAAATTTTC+AGG | + | chr3.2:81825089-81825108 | MS.gene055548:intron | 25.0% |
| !! | CATCGTTTATTTCATTTTGT+AGG | + | chr3.2:81824783-81824802 | MS.gene055548:intron | 25.0% |
| !! | GTTCTATGTTCAAATTTTCA+GGG | + | chr3.2:81825090-81825109 | MS.gene055548:intron | 25.0% |
| !!! | GTTTTAGAAAACGTACATTT+TGG | + | chr3.2:81825221-81825240 | MS.gene055548:CDS | 25.0% |
| !!! | TATTTTTGCATTTCTTTCGA+GGG | - | chr3.2:81825165-81825184 | None:intergenic | 25.0% |
| AAGTAAACACACACGAATAT+GGG | - | chr3.2:81825010-81825029 | None:intergenic | 30.0% | |
| AGGAAACATCATCATAAAGA+TGG | - | chr3.2:81824174-81824193 | None:intergenic | 30.0% | |
| AGGTAAGGTACACAAAAATA+TGG | - | chr3.2:81824636-81824655 | None:intergenic | 30.0% | |
| ATAAAACATCTATACCATCC+TGG | - | chr3.2:81824725-81824744 | None:intergenic | 30.0% | |
| ATTCATGATGATTATCCACA+AGG | + | chr3.2:81824405-81824424 | MS.gene055548:CDS | 30.0% | |
| CGACAGTAAAAGTTCAAAAA+TGG | - | chr3.2:81824698-81824717 | None:intergenic | 30.0% | |
| CTAGCTCATATGGTTTAATT+GGG | + | chr3.2:81825339-81825358 | MS.gene055548:CDS | 30.0% | |
| CTCGAAAGAAATGCAAAAAT+AGG | + | chr3.2:81825164-81825183 | MS.gene055548:CDS | 30.0% | |
| CTGTTCAATTAAAACTAGAG+AGG | - | chr3.2:81824663-81824682 | None:intergenic | 30.0% | |
| GGAAACATCATCATAAAGAT+GGG | - | chr3.2:81824173-81824192 | None:intergenic | 30.0% | |
| TAAAACATCTATACCATCCT+GGG | - | chr3.2:81824724-81824743 | None:intergenic | 30.0% | |
| TGGACATGTAATTCATTTGT+TGG | + | chr3.2:81825058-81825077 | MS.gene055548:intron | 30.0% | |
| ! | AATAATTACCTCAGAAGCAT+AGG | - | chr3.2:81824194-81824213 | None:intergenic | 30.0% |
| ! | GATATAGCTTGCGTAATTAA+TGG | + | chr3.2:81824856-81824875 | MS.gene055548:intron | 30.0% |
| ! | TCTAGCTCATATGGTTTAAT+TGG | + | chr3.2:81825338-81825357 | MS.gene055548:CDS | 30.0% |
| ! | TTATTTCATTTTGTAGGGAG+TGG | + | chr3.2:81824789-81824808 | MS.gene055548:intron | 30.0% |
| ! | TTTTCCGAATTGTCTCATTT+TGG | + | chr3.2:81824212-81824231 | MS.gene055548:intron | 30.0% |
| !! | ATTAAAACTAGAGAGGTATC+AGG | - | chr3.2:81824656-81824675 | None:intergenic | 30.0% |
| !!! | CTATTTTTGCATTTCTTTCG+AGG | - | chr3.2:81825166-81825185 | None:intergenic | 30.0% |
| CAAATGTGAAACAGTGATCA+AGG | - | chr3.2:81824549-81824568 | None:intergenic | 35.0% | |
| CAAGTAAACACACACGAATA+TGG | - | chr3.2:81825011-81825030 | None:intergenic | 35.0% | |
| GAGTGTTAACTATCTGTCTT+TGG | - | chr3.2:81824523-81824542 | None:intergenic | 35.0% | |
| ! | GATAAACTAGTCTGTGACAT+TGG | - | chr3.2:81825194-81825213 | None:intergenic | 35.0% |
| ! | TGGAGTGTTAATGTCATAGT+AGG | - | chr3.2:81824486-81824505 | None:intergenic | 35.0% |
| !! | ATGTCACAGACTAGTTTATC+CGG | + | chr3.2:81825194-81825213 | MS.gene055548:CDS | 35.0% |
| !! | ATTTTCAGGGAAGAGTGAAA+TGG | + | chr3.2:81825103-81825122 | MS.gene055548:intron | 35.0% |
| !!! | TACGTTTTCTAAAACTTCCC+CGG | - | chr3.2:81825216-81825235 | None:intergenic | 35.0% |
| AAACCATATGAGCTAGATGC+TGG | - | chr3.2:81825335-81825354 | None:intergenic | 40.0% | |
| AACAAGATTGTCACCAATGC+AGG | + | chr3.2:81825362-81825381 | MS.gene055548:CDS | 40.0% | |
| AGGAAACCAGCATGACATAT+AGG | - | chr3.2:81824466-81824485 | None:intergenic | 40.0% | |
| AGTACATGAGATCGAGAAGT+CGG | - | chr3.2:81824294-81824313 | None:intergenic | 40.0% | |
| TAGAGTCCTATATGTCATGC+TGG | + | chr3.2:81824457-81824476 | MS.gene055548:intron | 40.0% | |
| TGAATTATCACCCGAGATCA+TGG | + | chr3.2:81825291-81825310 | MS.gene055548:CDS | 40.0% | |
| TGATGATTATCCACAAGGTG+AGG | + | chr3.2:81824410-81824429 | MS.gene055548:intron | 40.0% | |
| TGATGTTTCCTATGCTTCTG+AGG | + | chr3.2:81824183-81824202 | MS.gene055548:CDS | 40.0% | |
| TGCAGAGGAATGTTGGATTA+AGG | + | chr3.2:81824370-81824389 | MS.gene055548:intron | 40.0% | |
| TTTCATGTGCAGAGGAATGT+TGG | + | chr3.2:81824363-81824382 | MS.gene055548:intron | 40.0% | |
| !! | AACTAGAGAGGTATCAGGTA+AGG | - | chr3.2:81824651-81824670 | None:intergenic | 40.0% |
| !! | TGTCACAGACTAGTTTATCC+GGG | + | chr3.2:81825195-81825214 | MS.gene055548:CDS | 40.0% |
| AGATGCGCTGAAGGATTCAA+AGG | + | chr3.2:81825262-81825281 | MS.gene055548:CDS | 45.0% | |
| AGTTGATTGACCTCACCTTG+TGG | - | chr3.2:81824423-81824442 | None:intergenic | 45.0% | |
| GATCATGGTCTGATGTGCAT+AGG | + | chr3.2:81825306-81825325 | MS.gene055548:CDS | 45.0% | |
| TGCACATCAGACCATGATCT+CGG | - | chr3.2:81825305-81825324 | None:intergenic | 45.0% | |
| ! | AACTTTTACTGTCGTTGCCC+AGG | + | chr3.2:81824704-81824723 | MS.gene055548:intron | 45.0% |
| ! | CTTGGACTTTTCATGTGCAG+AGG | + | chr3.2:81824355-81824374 | MS.gene055548:intron | 45.0% |
| !! | GTACATTTTGGTGCACAAGC+TGG | + | chr3.2:81825233-81825252 | MS.gene055548:CDS | 45.0% |
| !! | GTCACAGACTAGTTTATCCG+GGG | + | chr3.2:81825196-81825215 | MS.gene055548:CDS | 45.0% |
| AAGCAGTAGTAACACCCCTC+TGG | - | chr3.2:81824147-81824166 | None:intergenic | 50.0% | |
| AGTGAAATGGCAGCTTCACC+TGG | + | chr3.2:81825116-81825135 | MS.gene055548:CDS | 50.0% | |
| GAAGTGTGCAAAATGCCTCC+AGG | - | chr3.2:81825137-81825156 | None:intergenic | 50.0% | |
| GCACATCAGACCATGATCTC+GGG | - | chr3.2:81825304-81825323 | None:intergenic | 50.0% | |
| TTTACTGTCGTTGCCCAGGA+TGG | + | chr3.2:81824708-81824727 | MS.gene055548:intron | 50.0% | |
| ! | CAGCCAGCATCTAGCTCATA+TGG | + | chr3.2:81825329-81825348 | MS.gene055548:CDS | 50.0% |
| !! | TTTTGGTGCACAAGCTGGCA+TGG | + | chr3.2:81825238-81825257 | MS.gene055548:CDS | 50.0% |
| GAAATGGCAGCTTCACCTGG+AGG | + | chr3.2:81825119-81825138 | MS.gene055548:CDS | 55.0% | |
| TGGCATGGTAGATGCGCTGA+AGG | + | chr3.2:81825253-81825272 | MS.gene055548:CDS | 55.0% | |
| GCTTTCACGTGCGCCTGCAT+TGG | - | chr3.2:81825378-81825397 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 81824139 | 81825440 | 81824139 | ID=MS.gene055548 |
| chr3.2 | mRNA | 81824139 | 81825440 | 81824139 | ID=MS.gene055548.t1;Parent=MS.gene055548 |
| chr3.2 | exon | 81824139 | 81824204 | 81824139 | ID=MS.gene055548.t1.exon1;Parent=MS.gene055548.t1 |
| chr3.2 | CDS | 81824139 | 81824204 | 81824139 | ID=cds.MS.gene055548.t1;Parent=MS.gene055548.t1 |
| chr3.2 | exon | 81824375 | 81824426 | 81824375 | ID=MS.gene055548.t1.exon2;Parent=MS.gene055548.t1 |
| chr3.2 | CDS | 81824375 | 81824426 | 81824375 | ID=cds.MS.gene055548.t1;Parent=MS.gene055548.t1 |
| chr3.2 | exon | 81825111 | 81825440 | 81825111 | ID=MS.gene055548.t1.exon3;Parent=MS.gene055548.t1 |
| chr3.2 | CDS | 81825111 | 81825440 | 81825111 | ID=cds.MS.gene055548.t1;Parent=MS.gene055548.t1 |
| Gene Sequence |
| Protein sequence |
