Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055547.t1 | XP_003602750.2 | 96.6 | 149 | 4 | 1 | 1 | 149 | 6 | 153 | 6.30E-72 | 280 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055547.t1 | A0A072V1G3 | 96.6 | 149 | 4 | 1 | 1 | 149 | 6 | 153 | 4.5e-72 | 280.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049162 | MS.gene055547 | 0.941518 | 3.62E-101 | -1.69E-46 |
| MS.gene055547 | MS.gene055548 | 0.96088 | 4.65E-119 | -1.69E-46 |
| MS.gene055547 | MS.gene93871 | 0.801735 | 7.83E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055547.t1 | MTR_3g098620 | 96.644 | 149 | 4 | 1 | 1 | 149 | 6 | 153 | 2.96e-93 | 289 |
| MS.gene055547.t1 | MTR_3g098620 | 96.644 | 149 | 4 | 1 | 1 | 149 | 6 | 153 | 3.08e-93 | 289 |
| MS.gene055547.t1 | MTR_3g098620 | 96.644 | 149 | 4 | 1 | 1 | 149 | 6 | 153 | 6.02e-93 | 289 |
| MS.gene055547.t1 | MTR_8g100160 | 55.333 | 150 | 63 | 3 | 1 | 149 | 6 | 152 | 1.01e-44 | 156 |
| MS.gene055547.t1 | MTR_8g100160 | 55.333 | 150 | 63 | 3 | 1 | 149 | 6 | 152 | 1.02e-44 | 156 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAGCTCATATGGTTTAAT+TGG | 0.114750 | 3.2:+81966493 | MS.gene055547:CDS |
| TAATCATCATGAATAGATTT+TGG | 0.178888 | 3.2:-81965551 | None:intergenic |
| GAAACACATTCTTACACTTT+TGG | 0.261999 | 3.2:-81966588 | None:intergenic |
| TGCAGAGGAATGTTGGATTA+AGG | 0.349422 | 3.2:+81965525 | MS.gene055547:intron |
| CTAGCTCATATGGTTTAATT+GGG | 0.413117 | 3.2:+81966494 | MS.gene055547:CDS |
| CAGCCAGCATCTAGCTCATA+TGG | 0.414663 | 3.2:+81966484 | MS.gene055547:CDS |
| AACAAGATTGTCACCAATGC+AGG | 0.425656 | 3.2:+81966517 | MS.gene055547:CDS |
| AGGAAACATCATCATAAAGA+TGG | 0.434351 | 3.2:-81965324 | None:intergenic |
| ATGTCACAGACTAGTTTATC+CGG | 0.437906 | 3.2:+81966349 | MS.gene055547:CDS |
| GCTTTCACGTGCGCCTGCAT+TGG | 0.451678 | 3.2:-81966530 | None:intergenic |
| TGAATTATCACCCGAGATCA+TGG | 0.457507 | 3.2:+81966446 | MS.gene055547:CDS |
| GATAAACTAGTCTGTGACAT+TGG | 0.480263 | 3.2:-81966346 | None:intergenic |
| TTTCATGTGCAGAGGAATGT+TGG | 0.480325 | 3.2:+81965518 | MS.gene055547:intron |
| AGTGAAATGGCAGCTTCACC+TGG | 0.484170 | 3.2:+81966271 | MS.gene055547:CDS |
| TGCACATCAGACCATGATCT+CGG | 0.501021 | 3.2:-81966457 | None:intergenic |
| AATAATTACCTCAGAAGCAT+AGG | 0.505187 | 3.2:-81965344 | None:intergenic |
| AAACCATATGAGCTAGATGC+TGG | 0.540798 | 3.2:-81966487 | None:intergenic |
| GGAAACATCATCATAAAGAT+GGG | 0.545230 | 3.2:-81965323 | None:intergenic |
| TGTCACAGACTAGTTTATCC+GGG | 0.553964 | 3.2:+81966350 | MS.gene055547:CDS |
| AAGCAGTAGTAACACCCCTC+TGG | 0.563767 | 3.2:-81965297 | None:intergenic |
| GCACATCAGACCATGATCTC+GGG | 0.565376 | 3.2:-81966456 | None:intergenic |
| TGGCATGGTAGATGCGCTGA+AGG | 0.585812 | 3.2:+81966408 | MS.gene055547:CDS |
| AGATGCGCTGAAGGATTCAA+AGG | 0.602052 | 3.2:+81966417 | MS.gene055547:CDS |
| GATCATGGTCTGATGTGCAT+AGG | 0.608179 | 3.2:+81966461 | MS.gene055547:CDS |
| TGATGTTTCCTATGCTTCTG+AGG | 0.609927 | 3.2:+81965336 | MS.gene055547:CDS |
| GAAGTGTGCAAAATGCCTCC+AGG | 0.633750 | 3.2:-81966289 | None:intergenic |
| ATTCATGATGATTATCCACA+AGG | 0.660419 | 3.2:+81965560 | MS.gene055547:CDS |
| GTCACAGACTAGTTTATCCG+GGG | 0.680067 | 3.2:+81966351 | MS.gene055547:CDS |
| GAAATGGCAGCTTCACCTGG+AGG | 0.683059 | 3.2:+81966274 | MS.gene055547:CDS |
| TTTGAAATAATGAACCAGAG+GGG | 0.758778 | 3.2:+81965283 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GACATGTTTAGAAAAAAAAA+AGG | - | chr3.2:81965491-81965510 | None:intergenic | 20.0% |
| !! | TAAATAAGCAGTTCATTTAA+TGG | + | chr3.2:81966193-81966212 | MS.gene055547:intron | 20.0% |
| !! | TTAAATGAACTGCTTATTTA+TGG | - | chr3.2:81966193-81966212 | None:intergenic | 20.0% |
| !! | TTAGAATAAATAACTATGGA+TGG | + | chr3.2:81965905-81965924 | MS.gene055547:intron | 20.0% |
| !!! | ATCGTTTATTTCATTTTGTA+GGG | + | chr3.2:81965939-81965958 | MS.gene055547:intron | 20.0% |
| !!! | GCATTTAGAATAAATAACTA+TGG | + | chr3.2:81965901-81965920 | MS.gene055547:intron | 20.0% |
| !!! | GGTATTAATAGATTTCTTTT+TGG | + | chr3.2:81965965-81965984 | MS.gene055547:intron | 20.0% |
| !!! | GTTTTTAATCATGATGATAT+TGG | + | chr3.2:81966110-81966129 | MS.gene055547:intron | 20.0% |
| !!! | TAATCATCATGAATAGATTT+TGG | - | chr3.2:81965554-81965573 | None:intergenic | 20.0% |
| !!! | TTTTTTTTCTAAACATGTCT+TGG | + | chr3.2:81965492-81965511 | MS.gene055547:intron | 20.0% |
| ! | AAAAAAAGGCAATAACATTG+AGG | - | chr3.2:81965477-81965496 | None:intergenic | 25.0% |
| ! | AAAACCAAAATGAGACAATT+CGG | - | chr3.2:81965372-81965391 | None:intergenic | 25.0% |
| ! | CTTTGGTTAGTAGATATATA+TGG | - | chr3.2:81965661-81965680 | None:intergenic | 25.0% |
| ! | TAAAAAAACACTCAAAGTCT+TGG | - | chr3.2:81965413-81965432 | None:intergenic | 25.0% |
| !! | AGTTCTATGTTCAAATTTTC+AGG | + | chr3.2:81966244-81966263 | MS.gene055547:intron | 25.0% |
| !! | CATCGTTTATTTCATTTTGT+AGG | + | chr3.2:81965938-81965957 | MS.gene055547:intron | 25.0% |
| !! | GTTCTATGTTCAAATTTTCA+GGG | + | chr3.2:81966245-81966264 | MS.gene055547:intron | 25.0% |
| !!! | GTTTTAGAAAACGTACATTT+TGG | + | chr3.2:81966376-81966395 | MS.gene055547:CDS | 25.0% |
| !!! | TATTTTTGCATTTCTTTCGA+GGG | - | chr3.2:81966320-81966339 | None:intergenic | 25.0% |
| AAGTAAACACACACGAATAT+GGG | - | chr3.2:81966165-81966184 | None:intergenic | 30.0% | |
| AGGAAACATCATCATAAAGA+TGG | - | chr3.2:81965327-81965346 | None:intergenic | 30.0% | |
| AGGTAAGGTACACAAAAATA+TGG | - | chr3.2:81965791-81965810 | None:intergenic | 30.0% | |
| ATAAAACATCTATACCATCC+TGG | - | chr3.2:81965880-81965899 | None:intergenic | 30.0% | |
| ATTCATGATGATTATCCACA+AGG | + | chr3.2:81965560-81965579 | MS.gene055547:CDS | 30.0% | |
| CGACAGTAAAAGTTCAAAAA+TGG | - | chr3.2:81965853-81965872 | None:intergenic | 30.0% | |
| CTAGCTCATATGGTTTAATT+GGG | + | chr3.2:81966494-81966513 | MS.gene055547:CDS | 30.0% | |
| CTCGAAAGAAATGCAAAAAT+AGG | + | chr3.2:81966319-81966338 | MS.gene055547:CDS | 30.0% | |
| CTGTTCAATTAAAACTAGAG+AGG | - | chr3.2:81965818-81965837 | None:intergenic | 30.0% | |
| GGAAACATCATCATAAAGAT+GGG | - | chr3.2:81965326-81965345 | None:intergenic | 30.0% | |
| TAAAACATCTATACCATCCT+GGG | - | chr3.2:81965879-81965898 | None:intergenic | 30.0% | |
| TGGACATGTAATTCATTTGT+TGG | + | chr3.2:81966213-81966232 | MS.gene055547:intron | 30.0% | |
| ! | AATAATTACCTCAGAAGCAT+AGG | - | chr3.2:81965347-81965366 | None:intergenic | 30.0% |
| ! | GATATAGCTTGCGTAATTAA+TGG | + | chr3.2:81966011-81966030 | MS.gene055547:intron | 30.0% |
| ! | TCTAGCTCATATGGTTTAAT+TGG | + | chr3.2:81966493-81966512 | MS.gene055547:CDS | 30.0% |
| ! | TTATTTCATTTTGTAGGGAG+TGG | + | chr3.2:81965944-81965963 | MS.gene055547:intron | 30.0% |
| ! | TTTTCCGAATTGTCTCATTT+TGG | + | chr3.2:81965365-81965384 | MS.gene055547:intron | 30.0% |
| !! | ATTAAAACTAGAGAGGTATC+AGG | - | chr3.2:81965811-81965830 | None:intergenic | 30.0% |
| !!! | CTATTTTTGCATTTCTTTCG+AGG | - | chr3.2:81966321-81966340 | None:intergenic | 30.0% |
| CAAATGTGAAACAGTGATCA+AGG | - | chr3.2:81965704-81965723 | None:intergenic | 35.0% | |
| CAAGTAAACACACACGAATA+TGG | - | chr3.2:81966166-81966185 | None:intergenic | 35.0% | |
| GAGTGTTAACTATCTGTCTT+TGG | - | chr3.2:81965678-81965697 | None:intergenic | 35.0% | |
| ! | GATAAACTAGTCTGTGACAT+TGG | - | chr3.2:81966349-81966368 | None:intergenic | 35.0% |
| ! | TGGAGTGTTAATGTCATAGT+AGG | - | chr3.2:81965641-81965660 | None:intergenic | 35.0% |
| !! | ATGTCACAGACTAGTTTATC+CGG | + | chr3.2:81966349-81966368 | MS.gene055547:CDS | 35.0% |
| !! | ATTTTCAGGGAAGAGTGAAA+TGG | + | chr3.2:81966258-81966277 | MS.gene055547:intron | 35.0% |
| !!! | TACGTTTTCTAAAACTTCCC+CGG | - | chr3.2:81966371-81966390 | None:intergenic | 35.0% |
| AAACCATATGAGCTAGATGC+TGG | - | chr3.2:81966490-81966509 | None:intergenic | 40.0% | |
| AACAAGATTGTCACCAATGC+AGG | + | chr3.2:81966517-81966536 | MS.gene055547:CDS | 40.0% | |
| AGGAAACCAGCATGACATAT+AGG | - | chr3.2:81965621-81965640 | None:intergenic | 40.0% | |
| AGTACATGAGATCGAGAAGT+CGG | - | chr3.2:81965448-81965467 | None:intergenic | 40.0% | |
| TAGAGTCCTATATGTCATGC+TGG | + | chr3.2:81965612-81965631 | MS.gene055547:intron | 40.0% | |
| TGAATTATCACCCGAGATCA+TGG | + | chr3.2:81966446-81966465 | MS.gene055547:CDS | 40.0% | |
| TGATGATTATCCACAAGGTG+AGG | + | chr3.2:81965565-81965584 | MS.gene055547:intron | 40.0% | |
| TGATGTTTCCTATGCTTCTG+AGG | + | chr3.2:81965336-81965355 | MS.gene055547:CDS | 40.0% | |
| TGCAGAGGAATGTTGGATTA+AGG | + | chr3.2:81965525-81965544 | MS.gene055547:intron | 40.0% | |
| TTTCATGTGCAGAGGAATGT+TGG | + | chr3.2:81965518-81965537 | MS.gene055547:intron | 40.0% | |
| !! | AACTAGAGAGGTATCAGGTA+AGG | - | chr3.2:81965806-81965825 | None:intergenic | 40.0% |
| !! | TGTCACAGACTAGTTTATCC+GGG | + | chr3.2:81966350-81966369 | MS.gene055547:CDS | 40.0% |
| AGATGCGCTGAAGGATTCAA+AGG | + | chr3.2:81966417-81966436 | MS.gene055547:CDS | 45.0% | |
| AGTTGATTGACCTCACCTTG+TGG | - | chr3.2:81965578-81965597 | None:intergenic | 45.0% | |
| GATCATGGTCTGATGTGCAT+AGG | + | chr3.2:81966461-81966480 | MS.gene055547:CDS | 45.0% | |
| TGCACATCAGACCATGATCT+CGG | - | chr3.2:81966460-81966479 | None:intergenic | 45.0% | |
| ! | AACTTTTACTGTCGTTGCCC+AGG | + | chr3.2:81965859-81965878 | MS.gene055547:intron | 45.0% |
| ! | CTTGGACTTTTCATGTGCAG+AGG | + | chr3.2:81965510-81965529 | MS.gene055547:intron | 45.0% |
| !! | GTACATTTTGGTGCACAAGC+TGG | + | chr3.2:81966388-81966407 | MS.gene055547:CDS | 45.0% |
| !! | GTCACAGACTAGTTTATCCG+GGG | + | chr3.2:81966351-81966370 | MS.gene055547:CDS | 45.0% |
| AAGCAGTAGTAACACCCCTC+TGG | - | chr3.2:81965300-81965319 | None:intergenic | 50.0% | |
| AGTGAAATGGCAGCTTCACC+TGG | + | chr3.2:81966271-81966290 | MS.gene055547:CDS | 50.0% | |
| GAAGTGTGCAAAATGCCTCC+AGG | - | chr3.2:81966292-81966311 | None:intergenic | 50.0% | |
| GCACATCAGACCATGATCTC+GGG | - | chr3.2:81966459-81966478 | None:intergenic | 50.0% | |
| TTTACTGTCGTTGCCCAGGA+TGG | + | chr3.2:81965863-81965882 | MS.gene055547:intron | 50.0% | |
| ! | CAGCCAGCATCTAGCTCATA+TGG | + | chr3.2:81966484-81966503 | MS.gene055547:CDS | 50.0% |
| !! | TTTTGGTGCACAAGCTGGCA+TGG | + | chr3.2:81966393-81966412 | MS.gene055547:CDS | 50.0% |
| GAAATGGCAGCTTCACCTGG+AGG | + | chr3.2:81966274-81966293 | MS.gene055547:CDS | 55.0% | |
| TGGCATGGTAGATGCGCTGA+AGG | + | chr3.2:81966408-81966427 | MS.gene055547:CDS | 55.0% | |
| GCTTTCACGTGCGCCTGCAT+TGG | - | chr3.2:81966533-81966552 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 81965292 | 81966595 | 81965292 | ID=MS.gene055547 |
| chr3.2 | mRNA | 81965292 | 81966595 | 81965292 | ID=MS.gene055547.t1;Parent=MS.gene055547 |
| chr3.2 | exon | 81965292 | 81965357 | 81965292 | ID=MS.gene055547.t1.exon1;Parent=MS.gene055547.t1 |
| chr3.2 | CDS | 81965292 | 81965357 | 81965292 | ID=cds.MS.gene055547.t1;Parent=MS.gene055547.t1 |
| chr3.2 | exon | 81965530 | 81965581 | 81965530 | ID=MS.gene055547.t1.exon2;Parent=MS.gene055547.t1 |
| chr3.2 | CDS | 81965530 | 81965581 | 81965530 | ID=cds.MS.gene055547.t1;Parent=MS.gene055547.t1 |
| chr3.2 | exon | 81966266 | 81966595 | 81966266 | ID=MS.gene055547.t1.exon3;Parent=MS.gene055547.t1 |
| chr3.2 | CDS | 81966266 | 81966595 | 81966266 | ID=cds.MS.gene055547.t1;Parent=MS.gene055547.t1 |
| Gene Sequence |
| Protein sequence |
