AlfalfaGEDB | MS.gene055662

M. sativa cultivar XinJiangDaYe / MS.gene055662

KEGG: -
GO: -
PFAM: PF03168.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055662.t1	XP_013467879.1	95.7	209	9	0	1	209	1	209	9.70E-111	409.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055662.t1	Q9SRN0	61.2	209	79	1	3	209	1	209	1.4e-73	277.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055662.t1	B7FMW8	95.7	209	9	0	1	209	1	209	7.0e-111	409.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055662	MS.gene056166	0.813804	2.13E-51	-1.69E-46
MS.gene055662	MS.gene060401	0.808577	2.89E-50	-1.69E-46
MS.gene055662	MS.gene39203	0.800301	1.54E-48	-1.69E-46
MS.gene055662	MS.gene70047	0.816327	5.86E-52	-1.69E-46
MS.gene055662	MS.gene98208	0.83623	1.06E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055662.t1	MTR_1g057420	95.694	209	9	0	1	209	1	209	3.45e-141	392
MS.gene055662.t1	MTR_7g106050	72.857	210	56	1	1	209	1	210	6.23e-120	339
MS.gene055662.t1	MTR_2g086790	51.131	221	91	3	4	209	3	221	9.33e-75	225
MS.gene055662.t1	MTR_4g051880	51.142	219	97	3	1	209	1	219	1.50e-66	204
MS.gene055662.t1	MTR_7g106080	70.476	105	30	1	106	209	7	111	9.36e-52	162
MS.gene055662.t1	MTR_3g092790	40.845	142	83	1	43	184	49	189	5.96e-37	128
MS.gene055662.t1	MTR_7g118270	32.500	160	108	0	24	183	50	209	4.37e-29	108
MS.gene055662.t1	MTR_8g024250	32.258	155	105	0	23	177	101	255	1.80e-19	84.3
MS.gene055662.t1	MTR_1g105570	26.882	186	121	4	28	208	54	229	1.10e-15	73.2
MS.gene055662.t1	MTR_2g011350	27.211	147	102	3	33	177	37	180	1.91e-15	72.0
MS.gene055662.t1	MTR_6g071410	28.834	163	105	5	19	172	6	166	7.34e-14	68.2
MS.gene055662.t1	MTR_6g070890	28.834	163	105	5	19	172	6	166	7.34e-14	68.2
MS.gene055662.t1	MTR_6g070900	28.571	168	105	4	17	175	8	169	9.11e-14	67.8
MS.gene055662.t1	MTR_6g071400	28.571	168	105	4	17	175	8	169	9.11e-14	67.8
MS.gene055662.t1	MTR_6g471090	29.114	158	106	5	19	172	9	164	3.73e-13	65.9
MS.gene055662.t1	MTR_4g119428	28.313	166	108	4	30	185	65	229	5.32e-13	66.2
MS.gene055662.t1	MTR_1g057270	27.381	168	102	4	36	193	73	230	1.93e-12	64.7
MS.gene055662.t1	MTR_5g087760	23.171	164	120	3	20	177	67	230	2.56e-12	64.3
MS.gene055662.t1	MTR_7g105870	26.471	170	122	3	16	183	39	207	2.97e-12	63.9
MS.gene055662.t1	MTR_3g063310	27.059	170	120	4	34	201	83	250	1.07e-11	62.8
MS.gene055662.t1	MTR_6g471080	31.013	158	103	5	19	172	6	161	2.77e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055662.t1	AT3G11660	63.158	209	75	1	3	209	1	209	9.31e-103	295
MS.gene055662.t1	AT3G52470	59.223	206	84	0	4	209	3	208	3.59e-96	279
MS.gene055662.t1	AT2G35960	59.524	210	84	1	1	209	1	210	1.17e-87	257
MS.gene055662.t1	AT3G44220	54.067	209	93	1	1	209	1	206	2.87e-82	243
MS.gene055662.t1	AT4G09590	52.381	210	98	1	1	208	1	210	7.11e-80	238
MS.gene055662.t1	AT2G35970	52.857	210	97	1	1	208	1	210	7.43e-80	238
MS.gene055662.t1	AT5G06330	52.607	211	94	2	1	209	1	207	1.66e-72	219
MS.gene055662.t1	AT5G22200	48.357	213	103	3	1	209	1	210	9.02e-68	207
MS.gene055662.t1	AT5G53730	37.017	181	107	2	31	209	38	213	6.81e-43	144
MS.gene055662.t1	AT4G01410	33.721	172	114	0	18	189	42	213	1.27e-31	115
MS.gene055662.t1	AT5G05657	47.656	128	55	6	40	161	11	132	4.95e-26	98.2
MS.gene055662.t1	AT2G35980	30.460	174	114	4	8	177	29	199	5.49e-18	79.3
MS.gene055662.t1	AT5G22870	25.301	166	123	1	12	177	21	185	5.18e-17	76.3
MS.gene055662.t1	AT5G06320	28.571	168	119	1	20	186	48	215	6.82e-16	73.9
MS.gene055662.t1	AT1G65690	26.190	168	117	2	26	186	69	236	3.72e-13	66.6
MS.gene055662.t1	AT1G61760	25.000	192	131	6	18	207	41	221	4.21e-12	63.5
MS.gene055662.t1	AT4G05220	24.699	166	116	4	25	187	51	210	9.34e-11	59.7

Find 53 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACTCTTCCAAAGAATATTT+TGG	0.136144	1.4:+47982633	MS.gene055662:CDS
GTTTGGTCGCCGTTTGTTTA+TGG	0.184681	1.4:+47982947	MS.gene055662:CDS
AAGTTGGAGGTTAAGAAATT+TGG	0.271389	1.4:-47982779	None:intergenic
CAATGGTAGAGGTGTTGATA+TGG	0.281056	1.4:+47983144	MS.gene055662:CDS
TGATTGCCCAAAATATTCTT+TGG	0.316402	1.4:-47982640	None:intergenic
TACTAACAATTCTCCTCATA+TGG	0.326317	1.4:+47982684	MS.gene055662:CDS
TTGATTCACTTGATACTTAA+CGG	0.338488	1.4:-47983176	None:intergenic
GAAAGGCTCACAGAGTTAAA+AGG	0.378155	1.4:-47982989	None:intergenic
GTGATGCGTTGAAAGCATAT+AGG	0.416672	1.4:-47982754	None:intergenic
TGATGCGTTGAAAGCATATA+GGG	0.432610	1.4:-47982753	None:intergenic
TTTGTTATCGTTAAAGTTGA+CGG	0.448460	1.4:+47983037	MS.gene055662:CDS
GTCTTAGAATTGCCCATATG+AGG	0.455412	1.4:-47982697	None:intergenic
CGTCATACTCTTTGTGACCT+TGG	0.458862	1.4:-47982925	None:intergenic
ACAGAGTTAAAAGGAGCAAC+AGG	0.465129	1.4:-47982980	None:intergenic
TTAAAGTTGACGGTAGAGTT+CGG	0.467702	1.4:+47983047	MS.gene055662:CDS
TCTTTGTGACCTTGGTAAGA+AGG	0.469412	1.4:-47982917	None:intergenic
GGTTACATCTAAGAGGATAA+AGG	0.479142	1.4:-47982732	None:intergenic
GGAGCCAACAGATTACTTAC+CGG	0.486207	1.4:+47982882	MS.gene055662:CDS
TGAAAGTGTGACTTGGAAGT+TGG	0.488981	1.4:-47982795	None:intergenic
TTACCAAGTGAGATATAAGC+AGG	0.501355	1.4:-47983121	None:intergenic
AATGGTAGAGGTGTTGATAT+GGG	0.507778	1.4:+47983145	MS.gene055662:CDS
AAGAAGGCGGTATGGCGGTC+CGG	0.510341	1.4:-47982901	None:intergenic
TATACACCGATGTGATCGTT+GGG	0.520242	1.4:-47982827	None:intergenic
CATATAGGGTTACATCTAAG+AGG	0.520339	1.4:-47982739	None:intergenic
TGCCCTGCTTATATCTCACT+TGG	0.524897	1.4:+47983118	MS.gene055662:CDS
ATATGAGGAGAATTGTTAGT+AGG	0.534804	1.4:-47982682	None:intergenic
TTCGAGATGAAAGTGTGACT+TGG	0.539270	1.4:-47982802	None:intergenic
CAACATCATCATGTCGGTGA+AGG	0.542685	1.4:+47982580	None:intergenic
GTCACAAAGAGTATGACGTT+TGG	0.543001	1.4:+47982930	MS.gene055662:CDS
ATATCATTTCCATAAACAAA+CGG	0.547490	1.4:-47982956	None:intergenic
GGTAGAGTTCGGTGGAAGGT+TGG	0.550692	1.4:+47983058	MS.gene055662:CDS
ACTAACAATTCTCCTCATAT+GGG	0.552423	1.4:+47982685	MS.gene055662:CDS
TGACGGTAGAGTTCGGTGGA+AGG	0.562286	1.4:+47983054	MS.gene055662:CDS
GGTAAGTAATCTGTTGGCTC+CGG	0.565971	1.4:-47982880	None:intergenic
TCACTTGGTAACAATGGTAG+AGG	0.567913	1.4:+47983133	MS.gene055662:CDS
AAGTGTGACTTGGAAGTTGG+AGG	0.568361	1.4:-47982792	None:intergenic
TTGGCTCCGGTAGGTCATGT+AGG	0.568437	1.4:-47982867	None:intergenic
GTATACACCGATGTGATCGT+TGG	0.572674	1.4:-47982828	None:intergenic
CTTTCACAAGATGAAAATAA+CGG	0.577234	1.4:+47983007	MS.gene055662:CDS
ACCTTGGTAAGAAGGCGGTA+TGG	0.578134	1.4:-47982909	None:intergenic
CGGTCCGGTAAGTAATCTGT+TGG	0.580629	1.4:-47982886	None:intergenic
TATATCTCACTTGGTAACAA+TGG	0.587729	1.4:+47983127	MS.gene055662:CDS
GCCATACCGCCTTCTTACCA+AGG	0.629346	1.4:+47982908	MS.gene055662:CDS
TTGTTAGTAGGACTAAGAAG+AGG	0.629767	1.4:-47982670	None:intergenic
TCGATACCTACATGACCTAC+CGG	0.639639	1.4:+47982861	MS.gene055662:CDS
ACATCTAAGAGGATAAAGGT+AGG	0.645304	1.4:-47982728	None:intergenic
TTGGTAAGAAGGCGGTATGG+CGG	0.656020	1.4:-47982906	None:intergenic
TTGTGACCTTGGTAAGAAGG+CGG	0.660513	1.4:-47982914	None:intergenic
CGAAATCCCAACGATCACAT+CGG	0.673622	1.4:+47982821	MS.gene055662:CDS
TACCAAGTGAGATATAAGCA+GGG	0.680419	1.4:-47983120	None:intergenic
AAGTTGACGGTAGAGTTCGG+TGG	0.681699	1.4:+47983050	MS.gene055662:CDS
AGTAATCTGTTGGCTCCGGT+AGG	0.690727	1.4:-47982876	None:intergenic
AAGGAGTGTGATCATCACAA+AGG	0.697465	1.4:+47982599	MS.gene055662:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATCATTTCCATAAACAAA+CGG	-	chr1.4:47982959-47982978	None:intergenic	20.0%
!	CTTTCACAAGATGAAAATAA+CGG	+	chr1.4:47983007-47983026	MS.gene055662:CDS	25.0%
!	TTGATTCACTTGATACTTAA+CGG	-	chr1.4:47983179-47983198	None:intergenic	25.0%
!!	ACTCTTCCAAAGAATATTTT+GGG	+	chr1.4:47982634-47982653	MS.gene055662:CDS	25.0%
!!	TACTCTTCCAAAGAATATTT+TGG	+	chr1.4:47982633-47982652	MS.gene055662:CDS	25.0%
!!	TTTGTTATCGTTAAAGTTGA+CGG	+	chr1.4:47983037-47983056	MS.gene055662:CDS	25.0%
	AAGTTGGAGGTTAAGAAATT+TGG	-	chr1.4:47982782-47982801	None:intergenic	30.0%
	ACTAACAATTCTCCTCATAT+GGG	+	chr1.4:47982685-47982704	MS.gene055662:CDS	30.0%
	ATATGAGGAGAATTGTTAGT+AGG	-	chr1.4:47982685-47982704	None:intergenic	30.0%
	TACTAACAATTCTCCTCATA+TGG	+	chr1.4:47982684-47982703	MS.gene055662:CDS	30.0%
	TGATTGCCCAAAATATTCTT+TGG	-	chr1.4:47982643-47982662	None:intergenic	30.0%
!	TATATCTCACTTGGTAACAA+TGG	+	chr1.4:47983127-47983146	MS.gene055662:CDS	30.0%
!!	AAGAATATTTTGGGCAATCA+TGG	+	chr1.4:47982643-47982662	MS.gene055662:CDS	30.0%
!!	CGTTATTTTCATCTTGTGAA+AGG	-	chr1.4:47983009-47983028	None:intergenic	30.0%
	ACATCTAAGAGGATAAAGGT+AGG	-	chr1.4:47982731-47982750	None:intergenic	35.0%
	CATATAGGGTTACATCTAAG+AGG	-	chr1.4:47982742-47982761	None:intergenic	35.0%
	GGTTACATCTAAGAGGATAA+AGG	-	chr1.4:47982735-47982754	None:intergenic	35.0%
	TACCAAGTGAGATATAAGCA+GGG	-	chr1.4:47983123-47983142	None:intergenic	35.0%
	TTAAAGTTGACGGTAGAGTT+CGG	+	chr1.4:47983047-47983066	MS.gene055662:CDS	35.0%
	TTACCAAGTGAGATATAAGC+AGG	-	chr1.4:47983124-47983143	None:intergenic	35.0%
	TTGTTAGTAGGACTAAGAAG+AGG	-	chr1.4:47982673-47982692	None:intergenic	35.0%
!	TGATGCGTTGAAAGCATATA+GGG	-	chr1.4:47982756-47982775	None:intergenic	35.0%
!!	AATGGTAGAGGTGTTGATAT+GGG	+	chr1.4:47983145-47983164	MS.gene055662:CDS	35.0%
!!	AGGATAAAGGTAGGTTTTGT+TGG	-	chr1.4:47982722-47982741	None:intergenic	35.0%
!!!	AAGGTTGGTGCTTTTATTTC+TGG	+	chr1.4:47983073-47983092	MS.gene055662:CDS	35.0%
	ACAGAGTTAAAAGGAGCAAC+AGG	-	chr1.4:47982983-47983002	None:intergenic	40.0%
	GAAAGGCTCACAGAGTTAAA+AGG	-	chr1.4:47982992-47983011	None:intergenic	40.0%
	GTCTTAGAATTGCCCATATG+AGG	-	chr1.4:47982700-47982719	None:intergenic	40.0%
	TATACACCGATGTGATCGTT+GGG	-	chr1.4:47982830-47982849	None:intergenic	40.0%
	TCTTTGTGACCTTGGTAAGA+AGG	-	chr1.4:47982920-47982939	None:intergenic	40.0%
!	AAGGAGTGTGATCATCACAA+AGG	+	chr1.4:47982599-47982618	MS.gene055662:CDS	40.0%
!	GTCACAAAGAGTATGACGTT+TGG	+	chr1.4:47982930-47982949	MS.gene055662:CDS	40.0%
!	GTGATGCGTTGAAAGCATAT+AGG	-	chr1.4:47982757-47982776	None:intergenic	40.0%
!	TCACTTGGTAACAATGGTAG+AGG	+	chr1.4:47983133-47983152	MS.gene055662:CDS	40.0%
!	TGAAAGTGTGACTTGGAAGT+TGG	-	chr1.4:47982798-47982817	None:intergenic	40.0%
!	TTCGAGATGAAAGTGTGACT+TGG	-	chr1.4:47982805-47982824	None:intergenic	40.0%
!!	CAATGGTAGAGGTGTTGATA+TGG	+	chr1.4:47983144-47983163	MS.gene055662:CDS	40.0%
	CGAAATCCCAACGATCACAT+CGG	+	chr1.4:47982821-47982840	MS.gene055662:CDS	45.0%
	CGTCATACTCTTTGTGACCT+TGG	-	chr1.4:47982928-47982947	None:intergenic	45.0%
	GGAGCCAACAGATTACTTAC+CGG	+	chr1.4:47982882-47982901	MS.gene055662:CDS	45.0%
	GTATACACCGATGTGATCGT+TGG	-	chr1.4:47982831-47982850	None:intergenic	45.0%
	GTTTGGTCGCCGTTTGTTTA+TGG	+	chr1.4:47982947-47982966	MS.gene055662:CDS	45.0%
	TCGATACCTACATGACCTAC+CGG	+	chr1.4:47982861-47982880	MS.gene055662:CDS	45.0%
	TGCCCTGCTTATATCTCACT+TGG	+	chr1.4:47983118-47983137	MS.gene055662:CDS	45.0%
	TTGTGACCTTGGTAAGAAGG+CGG	-	chr1.4:47982917-47982936	None:intergenic	45.0%
!	AAGTGTGACTTGGAAGTTGG+AGG	-	chr1.4:47982795-47982814	None:intergenic	45.0%
!!	GGTAAGTAATCTGTTGGCTC+CGG	-	chr1.4:47982883-47982902	None:intergenic	45.0%
	AAGTTGACGGTAGAGTTCGG+TGG	+	chr1.4:47983050-47983069	MS.gene055662:CDS	50.0%
	ACCTTGGTAAGAAGGCGGTA+TGG	-	chr1.4:47982912-47982931	None:intergenic	50.0%
	CGGTCCGGTAAGTAATCTGT+TGG	-	chr1.4:47982889-47982908	None:intergenic	50.0%
	TTGGTAAGAAGGCGGTATGG+CGG	-	chr1.4:47982909-47982928	None:intergenic	50.0%
!!	AGTAATCTGTTGGCTCCGGT+AGG	-	chr1.4:47982879-47982898	None:intergenic	50.0%
	GCCATACCGCCTTCTTACCA+AGG	+	chr1.4:47982908-47982927	MS.gene055662:CDS	55.0%
	TTGGCTCCGGTAGGTCATGT+AGG	-	chr1.4:47982870-47982889	None:intergenic	55.0%
!	GGTAGAGTTCGGTGGAAGGT+TGG	+	chr1.4:47983058-47983077	MS.gene055662:CDS	55.0%
!	TGACGGTAGAGTTCGGTGGA+AGG	+	chr1.4:47983054-47983073	MS.gene055662:CDS	55.0%
	AAGAAGGCGGTATGGCGGTC+CGG	-	chr1.4:47982904-47982923	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	47982590	47983219	47982590	ID=MS.gene055662
chr1.4	mRNA	47982590	47983219	47982590	ID=MS.gene055662.t1;Parent=MS.gene055662
chr1.4	exon	47982590	47983219	47982590	ID=MS.gene055662.t1.exon1;Parent=MS.gene055662.t1
chr1.4	CDS	47982590	47983219	47982590	ID=cds.MS.gene055662.t1;Parent=MS.gene055662.t1

Gene Sequence

>MS.gene055662

ATGTCGGTGAAGGAGTGTGATCATCACAAAGGAAGAAAAAGAATACTCTTCCAAAGAATATTTTGGGCAATCATGGTATTCCTCTTCTTAGTCCTACTAACAATTCTCCTCATATGGGCAATTCTAAGACCAACAAAACCTACCTTTATCCTCTTAGATGTAACCCTATATGCTTTCAACGCATCACAACCAAATTTCTTAACCTCCAACTTCCAAGTCACACTTTCATCTCGAAATCCCAACGATCACATCGGTGTATACTACGATCGTCTCGATACCTACATGACCTACCGGAGCCAACAGATTACTTACCGGACCGCCATACCGCCTTCTTACCAAGGTCACAAAGAGTATGACGTTTGGTCGCCGTTTGTTTATGGAAATGATATACCTGTTGCTCCTTTTAACTCTGTGAGCCTTTCACAAGATGAAAATAACGGTAATATGTTTGTTATCGTTAAAGTTGACGGTAGAGTTCGGTGGAAGGTTGGTGCTTTTATTTCTGGTCGTTATCATATACATGTTCGTTGCCCTGCTTATATCTCACTTGGTAACAATGGTAGAGGTGTTGATATGGGAGAAAACGCCGTTAAGTATCAAGTGAATCAACGTTGCTCCGTTAACGTTTGA

Protein sequence

>MS.gene055662.t1

MSVKECDHHKGRKRILFQRIFWAIMVFLFLVLLTILLIWAILRPTKPTFILLDVTLYAFNASQPNFLTSNFQVTLSSRNPNDHIGVYYDRLDTYMTYRSQQITYRTAIPPSYQGHKEYDVWSPFVYGNDIPVAPFNSVSLSQDENNGNMFVIVKVDGRVRWKVGAFISGRYHIHVRCPAYISLGNNGRGVDMGENAVKYQVNQRCSVNV