AlfalfaGEDB | MS.gene98208

M. sativa cultivar XinJiangDaYe / MS.gene98208

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98208.t1	XP_013441979.1	95.1	103	5	0	2	104	48	150	8.10E-50	206.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98208.t1	O22150	56.3	87	38	0	15	101	65	151	3.5e-25	115.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98208.t1	G7J151	95.1	103	5	0	2	104	48	150	5.8e-50	206.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049396	MS.gene98208	0.8602	2.44E-63	-1.69E-46
MS.gene050170	MS.gene98208	0.806325	8.69E-50	-1.69E-46
MS.gene050502	MS.gene98208	0.870886	1.04E-66	-1.69E-46
MS.gene051838	MS.gene98208	0.804431	2.17E-49	-1.69E-46
MS.gene051941	MS.gene98208	0.805551	1.26E-49	-1.69E-46
MS.gene052346	MS.gene98208	0.84149	4.60E-58	-1.69E-46
MS.gene052414	MS.gene98208	0.851079	1.12E-60	-1.69E-46
MS.gene052503	MS.gene98208	0.808547	2.94E-50	-1.69E-46
MS.gene052823	MS.gene98208	0.815379	9.53E-52	-1.69E-46
MS.gene052825	MS.gene98208	0.805312	1.42E-49	-1.69E-46
MS.gene053449	MS.gene98208	0.830928	2.23E-55	-1.69E-46
MS.gene053505	MS.gene98208	0.817098	3.93E-52	-1.69E-46
MS.gene053574	MS.gene98208	0.806436	8.24E-50	-1.69E-46
MS.gene053626	MS.gene98208	0.801517	8.67E-49	-1.69E-46
MS.gene055662	MS.gene98208	0.83623	1.06E-56	-1.69E-46
MS.gene055741	MS.gene98208	0.829263	5.68E-55	-1.69E-46
MS.gene055744	MS.gene98208	0.832501	9.12E-56	-1.69E-46
MS.gene055909	MS.gene98208	0.841213	5.44E-58	-1.69E-46
MS.gene055911	MS.gene98208	0.840565	8.05E-58	-1.69E-46
MS.gene056166	MS.gene98208	0.862762	4.03E-64	-1.69E-46
MS.gene056387	MS.gene98208	0.818621	1.78E-52	-1.69E-46
MS.gene056422	MS.gene98208	0.828328	9.56E-55	-1.69E-46
MS.gene056504	MS.gene98208	0.803436	3.49E-49	-1.69E-46
MS.gene057019	MS.gene98208	0.822516	2.27E-53	-1.69E-46
MS.gene057086	MS.gene98208	0.874976	4.43E-68	-1.69E-46
MS.gene057734	MS.gene98208	0.817539	3.13E-52	-1.69E-46
MS.gene057856	MS.gene98208	0.833241	5.97E-56	-1.69E-46
MS.gene059368	MS.gene98208	0.813877	2.05E-51	-1.69E-46
MS.gene059517	MS.gene98208	0.814057	1.87E-51	-1.69E-46
MS.gene059667	MS.gene98208	0.812362	4.40E-51	-1.69E-46
MS.gene059674	MS.gene98208	0.802335	5.89E-49	-1.69E-46
MS.gene059807	MS.gene98208	0.804692	1.91E-49	-1.69E-46
MS.gene06000	MS.gene98208	0.806927	6.49E-50	-1.69E-46
MS.gene060372	MS.gene98208	0.831645	1.48E-55	-1.69E-46
MS.gene060778	MS.gene98208	0.805971	1.03E-49	-1.69E-46
MS.gene060837	MS.gene98208	0.817347	3.46E-52	-1.69E-46
MS.gene06097	MS.gene98208	0.869682	2.58E-66	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98208.t1	MTR_0388s0020	95.146	103	5	0	2	104	48	150	6.73e-70	206
MS.gene98208.t1	MTR_4g025830	57.798	109	37	1	2	101	43	151	4.80e-39	128
MS.gene98208.t1	MTR_7g118010	60.294	68	26	1	28	95	22	88	1.37e-24	89.7
MS.gene98208.t1	MTR_3g092220	50.000	74	36	1	28	101	32	104	6.68e-21	80.5
MS.gene98208.t1	MTR_8g026730	55.882	68	29	1	28	95	26	92	1.13e-20	79.7
MS.gene98208.t1	MTR_3g084250	49.398	83	37	3	15	95	10	89	2.79e-19	75.9
MS.gene98208.t1	MTR_4g072240	43.678	87	44	3	11	95	7	90	3.97e-18	72.8
MS.gene98208.t1	MTR_4g072500	42.045	88	46	3	10	95	5	89	4.85e-18	72.8
MS.gene98208.t1	MTR_4g072570	45.783	83	40	3	15	95	10	89	5.97e-18	72.4
MS.gene98208.t1	MTR_4g072300	43.678	87	44	3	11	95	7	90	1.04e-17	71.6
MS.gene98208.t1	MTR_4g072530	40.909	88	47	3	10	95	5	89	1.35e-17	71.6
MS.gene98208.t1	MTR_4g072870	41.860	86	45	3	12	95	8	90	1.44e-17	71.2
MS.gene98208.t1	MTR_4g072980	38.043	92	49	3	5	95	8	92	2.38e-17	70.9
MS.gene98208.t1	MTR_4g072640	39.773	88	48	3	10	95	5	89	3.48e-17	70.5
MS.gene98208.t1	MTR_4g072220	40.909	88	47	3	11	96	7	91	3.98e-17	70.5
MS.gene98208.t1	MTR_4g072600	43.590	78	40	2	20	96	11	85	1.71e-16	68.6
MS.gene98208.t1	MTR_3g117640	44.595	74	38	2	28	100	33	104	2.07e-16	68.9
MS.gene98208.t1	MTR_4g072860	46.377	69	33	2	28	95	25	90	3.95e-16	67.8
MS.gene98208.t1	MTR_4g072330	38.542	96	49	3	1	95	1	87	1.01e-15	66.6
MS.gene98208.t1	MTR_3g084210	50.000	60	26	2	26	84	22	78	1.36e-15	66.2
MS.gene98208.t1	MTR_4g072850	44.928	69	34	2	28	95	26	91	1.37e-15	66.6
MS.gene98208.t1	MTR_4g072910	49.275	69	30	3	16	83	23	87	1.50e-15	66.6
MS.gene98208.t1	MTR_3g084230	50.000	60	26	2	26	84	22	78	1.65e-15	66.2
MS.gene98208.t1	MTR_8g076040	48.529	68	32	1	28	95	40	104	1.69e-15	66.6
MS.gene98208.t1	MTR_3g084160	51.667	60	25	2	26	84	22	78	2.34e-15	65.9
MS.gene98208.t1	MTR_4g072560	43.902	82	42	3	5	84	7	86	3.28e-15	65.5
MS.gene98208.t1	MTR_4g072190	38.298	94	52	3	4	95	10	99	3.48e-15	65.5
MS.gene98208.t1	MTR_4g072230	43.662	71	34	2	24	93	21	86	3.93e-15	65.1
MS.gene98208.t1	MTR_8g461420	50.000	60	26	2	26	84	22	78	3.95e-15	65.1
MS.gene98208.t1	MTR_3g117610	42.029	69	37	1	27	95	43	108	4.90e-15	65.5
MS.gene98208.t1	MTR_4g072310	40.541	74	40	2	23	95	20	90	5.48e-15	65.1
MS.gene98208.t1	MTR_4g072480	43.373	83	42	3	15	95	10	89	5.53e-15	64.7
MS.gene98208.t1	MTR_3g113310	42.857	70	37	1	26	95	39	105	5.58e-15	65.1
MS.gene98208.t1	MTR_3g084190	51.667	60	25	2	26	84	22	78	5.59e-15	64.7
MS.gene98208.t1	MTR_4g072270	43.836	73	37	2	23	94	20	89	6.05e-15	64.7
MS.gene98208.t1	MTR_3g117630	46.479	71	33	3	15	83	10	77	7.41e-15	64.7
MS.gene98208.t1	MTR_4g072880	40.476	84	45	3	12	93	8	88	7.93e-15	64.3
MS.gene98208.t1	MTR_5g021820	41.975	81	42	2	15	95	28	103	9.03e-15	64.7
MS.gene98208.t1	MTR_3g110038	41.250	80	43	2	15	93	11	87	1.04e-14	64.3
MS.gene98208.t1	MTR_4g072890	42.500	80	41	3	11	88	7	83	1.43e-14	63.9
MS.gene98208.t1	MTR_4g072370	38.542	96	49	3	1	95	1	87	1.46e-14	63.9
MS.gene98208.t1	MTR_4g072620	40.000	85	47	2	12	95	8	89	1.55e-14	63.5
MS.gene98208.t1	MTR_3g084240	40.000	80	44	2	15	93	11	87	2.12e-14	63.5
MS.gene98208.t1	MTR_4g072250	42.647	68	34	2	29	95	26	89	2.37e-14	63.2
MS.gene98208.t1	MTR_4g072260	37.079	89	51	3	11	97	7	92	2.86e-14	63.2
MS.gene98208.t1	MTR_3g084170	40.000	80	44	2	15	93	11	87	3.07e-14	63.2
MS.gene98208.t1	MTR_3g110045	50.877	57	24	2	29	84	84	137	3.08e-14	64.3
MS.gene98208.t1	MTR_4g072730	43.836	73	37	2	24	95	20	89	3.24e-14	62.8
MS.gene98208.t1	MTR_4g072820	42.647	68	35	2	29	95	20	84	3.33e-14	62.8
MS.gene98208.t1	MTR_3g084220	44.286	70	35	2	15	83	11	77	3.46e-14	62.8
MS.gene98208.t1	MTR_4g072740	42.857	77	40	2	20	95	11	84	4.08e-14	62.4
MS.gene98208.t1	MTR_3g084150	45.070	71	34	3	15	83	10	77	5.01e-14	62.4
MS.gene98208.t1	MTR_4g072680	45.570	79	38	3	15	91	10	85	5.64e-14	62.4
MS.gene98208.t1	MTR_3g084180	45.070	71	34	3	15	83	10	77	5.89e-14	62.4
MS.gene98208.t1	MTR_4g072660	44.444	72	36	2	24	94	15	83	6.62e-14	62.0
MS.gene98208.t1	MTR_4g072460	45.570	79	38	3	15	91	10	85	7.40e-14	62.0
MS.gene98208.t1	MTR_4g124750	43.662	71	35	2	23	91	61	128	7.44e-14	63.5
MS.gene98208.t1	MTR_4g072590	49.231	65	26	3	28	88	19	80	8.17e-14	61.6
MS.gene98208.t1	MTR_4g072940	46.154	65	31	2	28	91	56	117	8.31e-14	62.8
MS.gene98208.t1	MTR_2g043960	41.096	73	40	1	25	97	72	141	1.12e-13	63.2
MS.gene98208.t1	MTR_4g072830	42.105	76	40	2	22	96	16	88	1.25e-13	61.2
MS.gene98208.t1	MTR_4g072580	39.744	78	43	2	20	96	11	85	1.26e-13	61.2
MS.gene98208.t1	MTR_4g072630	44.776	67	33	2	19	84	11	74	1.28e-13	61.2
MS.gene98208.t1	MTR_8g461410	43.662	71	35	3	15	83	10	77	1.50e-13	61.2
MS.gene98208.t1	MTR_5g008370	42.029	69	36	2	28	95	24	89	2.83e-13	61.6
MS.gene98208.t1	MTR_3g110042	42.169	83	43	3	15	95	10	89	2.92e-13	60.5
MS.gene98208.t1	MTR_2g095430	43.860	57	32	0	35	91	52	108	3.13e-13	61.2
MS.gene98208.t1	MTR_1g009150	40.541	74	41	1	28	101	70	140	3.81e-13	62.4
MS.gene98208.t1	MTR_5g008360	38.961	77	43	2	20	95	7	80	4.23e-13	59.7
MS.gene98208.t1	MTR_3g084260	38.298	94	51	3	1	93	1	88	4.54e-13	60.1
MS.gene98208.t1	MTR_4g072610	37.647	85	49	2	13	96	3	84	4.76e-13	59.7
MS.gene98208.t1	MTR_4g057810	43.750	64	35	1	28	91	37	99	5.12e-13	60.5
MS.gene98208.t1	MTR_3g110032	40.741	81	43	3	15	93	11	88	5.57e-13	59.7
MS.gene98208.t1	MTR_4g072160	42.308	78	39	2	19	96	30	101	5.80e-13	60.8
MS.gene98208.t1	MTR_4g072930	41.026	78	40	3	13	88	29	102	5.90e-13	60.1
MS.gene98208.t1	MTR_4g072670	48.276	58	26	2	28	84	20	74	6.04e-13	59.7
MS.gene98208.t1	MTR_4g072540	42.029	69	36	2	28	95	20	85	6.04e-13	59.7
MS.gene98208.t1	MTR_4g072550	48.438	64	29	2	29	91	36	96	6.28e-13	60.1
MS.gene98208.t1	MTR_4g050700	41.667	84	42	3	1	83	1	78	7.63e-13	59.3
MS.gene98208.t1	MTR_2g012410	41.667	84	42	3	1	83	1	78	7.63e-13	59.3
MS.gene98208.t1	MTR_5g008350	44.737	76	37	3	13	86	3	75	9.30e-13	58.9
MS.gene98208.t1	MTR_4g072770	42.857	70	36	2	29	97	15	81	1.37e-12	58.5
MS.gene98208.t1	MTR_4g072465	41.791	67	35	2	19	84	11	74	1.77e-12	58.2
MS.gene98208.t1	MTR_4g072720	42.029	69	36	2	28	95	20	85	2.01e-12	58.2
MS.gene98208.t1	MTR_4g005320	42.857	63	33	1	29	91	70	129	2.26e-12	60.1
MS.gene98208.t1	MTR_1g024085	37.500	88	51	2	14	101	23	106	2.35e-12	59.3
MS.gene98208.t1	MTR_8g461210	40.909	66	36	1	26	91	32	94	2.68e-12	58.5
MS.gene98208.t1	MTR_4g072470	44.615	65	32	2	20	83	11	72	3.23e-12	57.8
MS.gene98208.t1	MTR_2g066830	32.558	86	55	1	13	98	42	124	3.34e-12	58.9
MS.gene98208.t1	MTR_4g072450	47.368	57	26	2	29	84	20	73	5.04e-12	57.0
MS.gene98208.t1	MTR_3g109160	37.500	88	50	2	6	91	44	128	9.02e-12	58.5
MS.gene98208.t1	MTR_7g118000	55.319	47	21	0	58	104	27	73	3.32e-11	55.8
MS.gene98208.t1	MTR_4g072280	45.312	64	31	2	11	73	7	67	3.43e-11	54.7
MS.gene98208.t1	MTR_4g072750	47.368	57	26	2	23	78	20	73	5.17e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98208.t1	AT2G45210	63.014	73	27	0	29	101	79	151	2.30e-33	113
MS.gene98208.t1	AT3G60690	46.226	106	49	2	2	99	53	158	1.68e-29	104
MS.gene98208.t1	AT4G12410	45.283	106	49	2	1	97	39	144	1.23e-26	96.7
MS.gene98208.t1	AT4G22620	55.000	80	35	1	19	97	68	147	2.59e-26	95.9
MS.gene98208.t1	AT2G46690	48.052	77	40	0	28	104	23	99	4.17e-22	84.0
MS.gene98208.t1	AT3G61900	54.412	68	31	0	28	95	29	96	1.56e-21	82.8
MS.gene98208.t1	AT4G00880	49.333	75	37	1	28	102	27	100	3.99e-20	79.0
MS.gene98208.t1	AT5G53590	41.772	79	44	1	28	104	45	123	3.83e-18	74.3
MS.gene98208.t1	AT4G34800	55.738	61	26	1	29	88	21	81	7.58e-18	72.4
MS.gene98208.t1	AT3G03820	43.333	90	47	2	7	95	8	94	1.28e-17	71.6
MS.gene98208.t1	AT4G34810	48.148	81	40	2	6	84	25	105	2.35e-17	71.6
MS.gene98208.t1	AT2G21210	42.308	78	43	2	23	99	22	98	4.35e-17	70.5
MS.gene98208.t1	AT4G38850	46.377	69	36	1	28	95	20	88	5.11e-17	70.1
MS.gene98208.t1	AT4G34790	55.738	61	25	2	29	88	39	98	1.09e-16	69.7
MS.gene98208.t1	AT2G21210	42.308	78	43	2	23	99	89	165	1.66e-16	70.9
MS.gene98208.t1	AT4G34770	46.250	80	40	3	21	99	27	104	2.03e-16	68.9
MS.gene98208.t1	AT2G21220	44.928	69	35	1	28	96	39	104	5.95e-16	67.8
MS.gene98208.t1	AT3G43120	35.644	101	62	1	4	104	54	151	9.23e-16	68.6
MS.gene98208.t1	AT3G03850	47.761	67	33	2	30	95	26	91	1.96e-15	66.2
MS.gene98208.t1	AT4G38825	41.111	90	43	3	7	95	8	88	4.77e-15	65.1
MS.gene98208.t1	AT4G34780	50.877	57	27	1	28	83	27	83	5.07e-15	65.5
MS.gene98208.t1	AT2G21200	47.692	65	31	2	20	83	12	74	5.32e-15	64.7
MS.gene98208.t1	AT4G38840	52.632	57	24	2	28	83	32	86	5.85e-15	65.1
MS.gene98208.t1	AT5G18080	46.154	78	33	4	7	83	8	77	6.77e-15	64.7
MS.gene98208.t1	AT3G20220	42.466	73	37	2	22	91	37	107	7.70e-15	65.1
MS.gene98208.t1	AT5G20810	44.615	65	33	1	25	89	75	136	1.77e-14	65.5
MS.gene98208.t1	AT5G18020	41.026	78	37	3	7	83	8	77	1.89e-14	63.5
MS.gene98208.t1	AT4G34760	43.478	69	36	1	27	95	41	106	2.08e-14	63.9
MS.gene98208.t1	AT4G13790	44.737	76	36	3	9	83	9	79	3.22e-14	63.2
MS.gene98208.t1	AT5G20810	44.615	65	33	1	25	89	75	136	3.55e-14	65.1
MS.gene98208.t1	AT3G12830	40.698	86	48	1	13	98	34	116	5.50e-14	63.5
MS.gene98208.t1	AT3G03840	44.928	69	36	2	28	95	26	93	6.79e-14	62.4
MS.gene98208.t1	AT5G18010	43.590	78	35	4	7	83	8	77	1.08e-13	61.6
MS.gene98208.t1	AT5G18060	44.737	76	33	4	9	83	10	77	1.09e-13	61.6
MS.gene98208.t1	AT5G18030	39.744	78	36	3	7	83	8	75	1.17e-13	61.6
MS.gene98208.t1	AT5G18050	35.556	90	49	3	7	95	8	89	1.19e-13	61.6
MS.gene98208.t1	AT3G03830	45.588	68	36	1	29	95	24	91	1.64e-13	61.2
MS.gene98208.t1	AT4G38860	44.286	70	35	2	27	95	38	104	3.68e-13	60.5
MS.gene98208.t1	AT1G19830	43.243	74	35	2	22	93	41	109	4.08e-13	60.8
MS.gene98208.t1	AT1G75580	42.254	71	34	2	27	95	42	107	4.69e-13	60.5
MS.gene98208.t1	AT1G56150	41.096	73	40	1	29	101	38	107	1.39e-12	59.3
MS.gene98208.t1	AT2G18010	39.506	81	45	2	3	83	23	99	2.05e-12	58.9
MS.gene98208.t1	AT5G66260	47.273	55	26	1	29	83	34	85	2.29e-12	58.5
MS.gene98208.t1	AT4G36110	48.214	56	26	1	28	83	39	91	3.09e-12	58.2
MS.gene98208.t1	AT5G10990	36.047	86	52	1	14	99	26	108	7.20e-12	58.2
MS.gene98208.t1	AT4G34750	37.931	87	48	2	21	104	26	109	1.02e-11	57.8
MS.gene98208.t1	AT4G34750	37.931	87	48	2	21	104	26	109	1.02e-11	57.8
MS.gene98208.t1	AT2G28085	40.678	59	35	0	35	93	62	120	1.26e-11	57.0
MS.gene98208.t1	AT1G75590	41.892	74	37	2	16	89	32	99	2.56e-11	57.0
MS.gene98208.t1	AT4G31320	36.486	74	44	1	28	101	79	149	9.88e-11	56.2

Find 33 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAAAGAGGAAGAGATAAAT+AGG	0.176068	7.1:-57011573	MS.gene98208:CDS
CCTTTGGCACGCTGAATTTC+GGG	0.180419	7.1:+57011620	None:intergenic
CCCTTTGGCACGCTGAATTT+CGG	0.193021	7.1:+57011619	None:intergenic
TACACAACCATTTGTCCCTT+TGG	0.333200	7.1:+57011604	None:intergenic
AAAGATGTTGAAGAGGAGTA+TGG	0.344907	7.1:-57011499	MS.gene98208:CDS
TGACCTGTTGAAAAGCTTCA+TGG	0.350356	7.1:+57011666	None:intergenic
TTGCATCAGGTTCCAGCAAT+TGG	0.352794	7.1:-57011408	MS.gene98208:CDS
TACGATACCATGTAGGTTTA+CGG	0.360176	7.1:-57011455	MS.gene98208:CDS
GGAAGAGATAAATAGGGTTA+TGG	0.394223	7.1:-57011566	MS.gene98208:CDS
GAAAGAGGAAGAGATAAATA+GGG	0.396870	7.1:-57011572	MS.gene98208:CDS
GCTGGAACCTGATGCAATCC+AGG	0.450641	7.1:+57011414	None:intergenic
AGGTCAAGTTATACTCGGTT+AGG	0.455077	7.1:-57011649	MS.gene98208:CDS
TAAGCAATTCACTAAACAAT+GGG	0.459340	7.1:+57011521	None:intergenic
ATCAAGACCTGGATTGCATC+AGG	0.485980	7.1:-57011421	MS.gene98208:CDS
CAGCGTGCCAAAGGGACAAA+TGG	0.489582	7.1:-57011611	MS.gene98208:CDS
GTATCGTAATCCCACCTTGA+TGG	0.497196	7.1:+57011470	None:intergenic
CCCGAAATTCAGCGTGCCAA+AGG	0.504666	7.1:-57011620	MS.gene98208:CDS
TGGCACGCTGAATTTCGGGC+TGG	0.523303	7.1:+57011624	None:intergenic
GGGACAAATGGTTGTGTATG+TGG	0.525067	7.1:-57011599	MS.gene98208:CDS
CCGAAATTCAGCGTGCCAAA+GGG	0.533551	7.1:-57011619	MS.gene98208:CDS
TGTGTATGTGGGACAGAAAG+AGG	0.554416	7.1:-57011587	MS.gene98208:CDS
TTAAGCAATTCACTAAACAA+TGG	0.555508	7.1:+57011520	None:intergenic
ATTGCTTAAAGATGTTGAAG+AGG	0.557241	7.1:-57011506	MS.gene98208:CDS
GGGTGGTTAAAGTATATCAC+TGG	0.571494	7.1:+57011541	None:intergenic
AGTTTGAACGGATCAAGACC+TGG	0.576336	7.1:-57011432	MS.gene98208:CDS
GTAGGTTTACGGAGTTTGAA+CGG	0.580984	7.1:-57011444	MS.gene98208:CDS
TGGCATCCATCGTTAGCTTG+CGG	0.581368	7.1:+57011686	None:intergenic
TCAACAGGTCAAGTTATACT+CGG	0.582354	7.1:-57011654	MS.gene98208:CDS
TCAAACTCCGTAAACCTACA+TGG	0.593678	7.1:+57011448	None:intergenic
GCAATTCACTAAACAATGGG+TGG	0.601342	7.1:+57011524	None:intergenic
GTGGGATTACGATACCATGT+AGG	0.627453	7.1:-57011462	MS.gene98208:CDS
AATGGGCCGCAAGCTAACGA+TGG	0.636715	7.1:-57011692	MS.gene98208:CDS
GGACAAATGGTTGTGTATGT+GGG	0.647999	7.1:-57011598	MS.gene98208:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TAAGCAATTCACTAAACAAT+GGG	+	chr7.1:57011572-57011591	None:intergenic	25.0%
!	TTAAGCAATTCACTAAACAA+TGG	+	chr7.1:57011573-57011592	None:intergenic	25.0%
	AGAAAGAGGAAGAGATAAAT+AGG	-	chr7.1:57011517-57011536	MS.gene98208:CDS	30.0%
	ATTGCTTAAAGATGTTGAAG+AGG	-	chr7.1:57011584-57011603	MS.gene98208:CDS	30.0%
	GAAAGAGGAAGAGATAAATA+GGG	-	chr7.1:57011518-57011537	MS.gene98208:CDS	30.0%
	AAAGATGTTGAAGAGGAGTA+TGG	-	chr7.1:57011591-57011610	MS.gene98208:CDS	35.0%
	GGAAGAGATAAATAGGGTTA+TGG	-	chr7.1:57011524-57011543	MS.gene98208:CDS	35.0%
	TACGATACCATGTAGGTTTA+CGG	-	chr7.1:57011635-57011654	MS.gene98208:CDS	35.0%
	TCAACAGGTCAAGTTATACT+CGG	-	chr7.1:57011436-57011455	MS.gene98208:CDS	35.0%
!!!	ATGGTTTTAACCATCAAGGT+GGG	-	chr7.1:57011610-57011629	MS.gene98208:CDS	35.0%
!!!	GAGTATGGTTTTAACCATCA+AGG	-	chr7.1:57011606-57011625	MS.gene98208:CDS	35.0%
!!!	TATGGTTTTAACCATCAAGG+TGG	-	chr7.1:57011609-57011628	MS.gene98208:CDS	35.0%
	AGGTCAAGTTATACTCGGTT+AGG	-	chr7.1:57011441-57011460	MS.gene98208:CDS	40.0%
	GCAATTCACTAAACAATGGG+TGG	+	chr7.1:57011569-57011588	None:intergenic	40.0%
	GGACAAATGGTTGTGTATGT+GGG	-	chr7.1:57011492-57011511	MS.gene98208:CDS	40.0%
	GGGTGGTTAAAGTATATCAC+TGG	+	chr7.1:57011552-57011571	None:intergenic	40.0%
	GTAGGTTTACGGAGTTTGAA+CGG	-	chr7.1:57011646-57011665	MS.gene98208:CDS	40.0%
	TACACAACCATTTGTCCCTT+TGG	+	chr7.1:57011489-57011508	None:intergenic	40.0%
	TCAAACTCCGTAAACCTACA+TGG	+	chr7.1:57011645-57011664	None:intergenic	40.0%
	TGACCTGTTGAAAAGCTTCA+TGG	+	chr7.1:57011427-57011446	None:intergenic	40.0%
!	ATGCCATGAAGCTTTTCAAC+AGG	-	chr7.1:57011421-57011440	MS.gene98208:CDS	40.0%
	AGTTTGAACGGATCAAGACC+TGG	-	chr7.1:57011658-57011677	MS.gene98208:CDS	45.0%
	ATCAAGACCTGGATTGCATC+AGG	-	chr7.1:57011669-57011688	MS.gene98208:CDS	45.0%
	GGGACAAATGGTTGTGTATG+TGG	-	chr7.1:57011491-57011510	MS.gene98208:CDS	45.0%
	GTATCGTAATCCCACCTTGA+TGG	+	chr7.1:57011623-57011642	None:intergenic	45.0%
	GTGGGATTACGATACCATGT+AGG	-	chr7.1:57011628-57011647	MS.gene98208:CDS	45.0%
	TGTGTATGTGGGACAGAAAG+AGG	-	chr7.1:57011503-57011522	MS.gene98208:CDS	45.0%
	TTGCATCAGGTTCCAGCAAT+TGG	-	chr7.1:57011682-57011701	MS.gene98208:CDS	45.0%
	CCCTTTGGCACGCTGAATTT+CGG	+	chr7.1:57011474-57011493	None:intergenic	50.0%
	CCGAAATTCAGCGTGCCAAA+GGG	-	chr7.1:57011471-57011490	MS.gene98208:CDS	50.0%
	CCTTTGGCACGCTGAATTTC+GGG	+	chr7.1:57011473-57011492	None:intergenic	50.0%
!	TGGCATCCATCGTTAGCTTG+CGG	+	chr7.1:57011407-57011426	None:intergenic	50.0%
	CAGCGTGCCAAAGGGACAAA+TGG	-	chr7.1:57011479-57011498	MS.gene98208:CDS	55.0%
	CCCGAAATTCAGCGTGCCAA+AGG	-	chr7.1:57011470-57011489	MS.gene98208:CDS	55.0%
	GCTGGAACCTGATGCAATCC+AGG	+	chr7.1:57011679-57011698	None:intergenic	55.0%
	TGGCACGCTGAATTTCGGGC+TGG	+	chr7.1:57011469-57011488	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	57011399	57011713	57011399	ID=MS.gene98208
chr7.1	mRNA	57011399	57011713	57011399	ID=MS.gene98208.t1;Parent=MS.gene98208
chr7.1	exon	57011399	57011713	57011399	ID=MS.gene98208.t1.exon1;Parent=MS.gene98208.t1
chr7.1	CDS	57011399	57011713	57011399	ID=cds.MS.gene98208.t1;Parent=MS.gene98208.t1

Gene Sequence	>MS.gene98208 ATGGGCCGCAAGCTAACGATGGATGCCATGAAGCTTTTCAACAGGTCAAGTTATACTCGGTTAGGATCCAGCCCGAAATTCAGCGTGCCAAAGGGACAAATGGTTGTGTATGTGGGACAGAAAGAGGAAGAGATAAATAGGGTTATGGTGCCAGTGATATACTTTAACCACCCATTGTTTAGTGAATTGCTTAAAGATGTTGAAGAGGAGTATGGTTTTAACCATCAAGGTGGGATTACGATACCATGTAGGTTTACGGAGTTTGAACGGATCAAGACCTGGATTGCATCAGGTTCCAGCAATTGGACCAGTTGA
Protein sequence	>MS.gene98208.t1 MGRKLTMDAMKLFNRSSYTRLGSSPKFSVPKGQMVVYVGQKEEEINRVMVPVIYFNHPLFSELLKDVEEEYGFNHQGGITIPCRFTEFERIKTWIASGSSNWTS