Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060401.t1 | XP_003594968.1 | 93.9 | 442 | 27 | 0 | 1 | 442 | 1 | 442 | 3.10E-244 | 854 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060401.t1 | Q39048 | 40.6 | 436 | 219 | 14 | 7 | 425 | 1 | 413 | 7.7e-82 | 305.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060401.t1 | G7IN16 | 93.9 | 442 | 27 | 0 | 1 | 442 | 1 | 442 | 2.3e-244 | 854.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050375 | MS.gene060401 | 0.826776 | 2.25E-54 | -1.69E-46 |
| MS.gene05335 | MS.gene060401 | 0.813943 | 1.98E-51 | -1.69E-46 |
| MS.gene053449 | MS.gene060401 | 0.81568 | 8.16E-52 | -1.69E-46 |
| MS.gene053813 | MS.gene060401 | 0.811895 | 5.57E-51 | -1.69E-46 |
| MS.gene055662 | MS.gene060401 | 0.808577 | 2.89E-50 | -1.69E-46 |
| MS.gene055909 | MS.gene060401 | 0.803629 | 3.18E-49 | -1.69E-46 |
| MS.gene055911 | MS.gene060401 | 0.811577 | 6.53E-51 | -1.69E-46 |
| MS.gene056422 | MS.gene060401 | 0.834935 | 2.25E-56 | -1.69E-46 |
| MS.gene057019 | MS.gene060401 | 0.801277 | 9.71E-49 | -1.69E-46 |
| MS.gene058995 | MS.gene060401 | 0.804722 | 1.89E-49 | -1.69E-46 |
| MS.gene059807 | MS.gene060401 | 0.810085 | 1.37E-50 | -1.69E-46 |
| MS.gene060372 | MS.gene060401 | 0.802596 | 5.20E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene060837 | 0.819423 | 1.17E-52 | -1.69E-46 |
| MS.gene060401 | MS.gene06097 | 0.801588 | 8.39E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene061718 | 0.814826 | 1.26E-51 | -1.69E-46 |
| MS.gene060401 | MS.gene064292 | 0.802635 | 5.11E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene065130 | 0.834226 | 3.39E-56 | -1.69E-46 |
| MS.gene060401 | MS.gene065699 | 0.810222 | 1.28E-50 | -1.69E-46 |
| MS.gene060401 | MS.gene06644 | 0.824986 | 5.99E-54 | -1.69E-46 |
| MS.gene060401 | MS.gene06751 | 0.805454 | 1.32E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene06858 | 0.819284 | 1.26E-52 | -1.69E-46 |
| MS.gene060401 | MS.gene068688 | 0.811686 | 6.18E-51 | -1.69E-46 |
| MS.gene060401 | MS.gene069435 | 0.803318 | 3.69E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene069717 | 0.808335 | 3.26E-50 | -1.69E-46 |
| MS.gene060401 | MS.gene070749 | 0.817092 | 3.94E-52 | -1.69E-46 |
| MS.gene060401 | MS.gene071757 | 0.801258 | 9.80E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene071758 | 0.804186 | 2.44E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene072799 | 0.809358 | 1.97E-50 | -1.69E-46 |
| MS.gene060401 | MS.gene20220 | 0.83062 | 2.65E-55 | -1.69E-46 |
| MS.gene060401 | MS.gene21731 | 0.817201 | 3.73E-52 | -1.69E-46 |
| MS.gene060401 | MS.gene22632 | 0.802999 | 4.30E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene22673 | 0.814745 | 1.32E-51 | -1.69E-46 |
| MS.gene060401 | MS.gene24611 | 0.819414 | 1.18E-52 | -1.69E-46 |
| MS.gene060401 | MS.gene28132 | 0.807191 | 5.70E-50 | -1.69E-46 |
| MS.gene060401 | MS.gene28312 | 0.810215 | 1.29E-50 | -1.69E-46 |
| MS.gene060401 | MS.gene28441 | 0.816644 | 4.97E-52 | -1.69E-46 |
| MS.gene060401 | MS.gene30733 | 0.801302 | 9.60E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene32865 | 0.827159 | 1.82E-54 | -1.69E-46 |
| MS.gene060401 | MS.gene34800 | 0.807263 | 5.51E-50 | -1.69E-46 |
| MS.gene060401 | MS.gene35265 | 0.80456 | 2.04E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene35623 | 0.812463 | 4.19E-51 | -1.69E-46 |
| MS.gene060401 | MS.gene35710 | 0.803495 | 3.39E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene37919 | 0.826256 | 3.00E-54 | -1.69E-46 |
| MS.gene060401 | MS.gene39203 | 0.806728 | 7.15E-50 | -1.69E-46 |
| MS.gene060401 | MS.gene39300 | 0.811939 | 5.45E-51 | -1.69E-46 |
| MS.gene060401 | MS.gene42746 | 0.809604 | 1.74E-50 | -1.69E-46 |
| MS.gene060401 | MS.gene46956 | 0.800985 | 1.11E-48 | -1.69E-46 |
| MS.gene060401 | MS.gene47398 | 0.81312 | 3.01E-51 | -1.69E-46 |
| MS.gene060401 | MS.gene47869 | 0.801217 | 9.99E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene48585 | 0.831866 | 1.31E-55 | -1.69E-46 |
| MS.gene060401 | MS.gene52324 | 0.801229 | 9.94E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene57694 | 0.860433 | 2.07E-63 | -1.69E-46 |
| MS.gene060401 | MS.gene58344 | 0.810727 | 9.98E-51 | -1.69E-46 |
| MS.gene060401 | MS.gene58434 | 0.82363 | 1.25E-53 | -1.69E-46 |
| MS.gene060401 | MS.gene61160 | 0.819391 | 1.19E-52 | -1.69E-46 |
| MS.gene060401 | MS.gene61506 | 0.815961 | 7.07E-52 | -1.69E-46 |
| MS.gene060401 | MS.gene62289 | 0.820061 | 8.37E-53 | -1.69E-46 |
| MS.gene060401 | MS.gene62743 | 0.824676 | 7.10E-54 | -1.69E-46 |
| MS.gene060401 | MS.gene63115 | 0.813673 | 2.27E-51 | -1.69E-46 |
| MS.gene060401 | MS.gene65057 | 0.809093 | 2.24E-50 | -1.69E-46 |
| MS.gene060401 | MS.gene69489 | 0.819225 | 1.30E-52 | -1.69E-46 |
| MS.gene060401 | MS.gene70167 | 0.832785 | 7.75E-56 | -1.69E-46 |
| MS.gene060401 | MS.gene71287 | 0.844383 | 7.82E-59 | -1.69E-46 |
| MS.gene060401 | MS.gene71665 | 0.816096 | 6.60E-52 | -1.69E-46 |
| MS.gene060401 | MS.gene71666 | 0.831592 | 1.53E-55 | -1.69E-46 |
| MS.gene060401 | MS.gene71667 | 0.824496 | 7.82E-54 | -1.69E-46 |
| MS.gene060401 | MS.gene71836 | 0.831428 | 1.68E-55 | -1.69E-46 |
| MS.gene060401 | MS.gene71861 | 0.814416 | 1.56E-51 | -1.69E-46 |
| MS.gene060401 | MS.gene72470 | 0.81131 | 7.46E-51 | -1.69E-46 |
| MS.gene060401 | MS.gene72474 | 0.817959 | 2.52E-52 | -1.69E-46 |
| MS.gene060401 | MS.gene73546 | 0.801219 | 9.98E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene73665 | 0.849438 | 3.23E-60 | -1.69E-46 |
| MS.gene060401 | MS.gene76300 | 0.84427 | 8.39E-59 | -1.69E-46 |
| MS.gene060401 | MS.gene77460 | 0.825936 | 3.57E-54 | -1.69E-46 |
| MS.gene060401 | MS.gene77675 | 0.804527 | 2.07E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene79400 | 0.805669 | 1.19E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene79947 | 0.82024 | 7.61E-53 | -1.69E-46 |
| MS.gene060401 | MS.gene81523 | 0.806545 | 7.81E-50 | -1.69E-46 |
| MS.gene060401 | MS.gene81877 | 0.813998 | 1.93E-51 | -1.69E-46 |
| MS.gene060401 | MS.gene84397 | 0.808071 | 3.71E-50 | -1.69E-46 |
| MS.gene060401 | MS.gene84575 | 0.834614 | 2.71E-56 | -1.69E-46 |
| MS.gene060401 | MS.gene85059 | 0.800794 | 1.22E-48 | -1.69E-46 |
| MS.gene060401 | MS.gene86102 | 0.803033 | 4.23E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene87888 | 0.805143 | 1.54E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene88978 | 0.801514 | 8.69E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene91599 | 0.81257 | 3.97E-51 | -1.69E-46 |
| MS.gene060401 | MS.gene92064 | 0.818346 | 2.06E-52 | -1.69E-46 |
| MS.gene060401 | MS.gene93043 | 0.844012 | 9.84E-59 | -1.69E-46 |
| MS.gene060401 | MS.gene93095 | 0.804495 | 2.10E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene94035 | 0.822762 | 1.99E-53 | -1.69E-46 |
| MS.gene060401 | MS.gene95695 | 0.80388 | 2.82E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene95806 | 0.803195 | 3.91E-49 | -1.69E-46 |
| MS.gene060401 | MS.gene96657 | 0.817961 | 2.51E-52 | -1.69E-46 |
| MS.gene060401 | MS.gene99674 | 0.804864 | 1.76E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene08811 | MS.gene060401 | PPI |
| MS.gene09279 | MS.gene060401 | PPI |
| MS.gene08966 | MS.gene060401 | PPI |
| MS.gene023496 | MS.gene060401 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060401.t1 | MTR_2g036780 | 93.891 | 442 | 27 | 0 | 1 | 442 | 1 | 442 | 0.0 | 859 |
| MS.gene060401.t1 | MTR_3g100150 | 77.027 | 444 | 96 | 3 | 1 | 441 | 1 | 441 | 0.0 | 699 |
| MS.gene060401.t1 | MTR_1g035890 | 72.146 | 438 | 117 | 3 | 1 | 436 | 1 | 435 | 0.0 | 637 |
| MS.gene060401.t1 | MTR_8g081600 | 34.559 | 272 | 161 | 6 | 164 | 429 | 1 | 261 | 3.10e-40 | 145 |
| MS.gene060401.t1 | MTR_8g081480 | 51.145 | 131 | 62 | 2 | 16 | 145 | 16 | 145 | 4.23e-35 | 127 |
| MS.gene060401.t1 | MTR_7g114960 | 25.112 | 446 | 271 | 17 | 19 | 424 | 15 | 437 | 1.01e-26 | 112 |
| MS.gene060401.t1 | MTR_1g011660 | 25.829 | 422 | 264 | 14 | 35 | 424 | 27 | 431 | 7.89e-23 | 100 |
| MS.gene060401.t1 | MTR_8g079970 | 25.000 | 272 | 174 | 8 | 19 | 276 | 10 | 265 | 1.71e-14 | 75.5 |
| MS.gene060401.t1 | MTR_8g079950 | 28.916 | 166 | 110 | 4 | 19 | 180 | 10 | 171 | 3.58e-13 | 71.6 |
| MS.gene060401.t1 | MTR_8g096530 | 25.368 | 272 | 174 | 9 | 18 | 273 | 13 | 271 | 1.21e-12 | 69.7 |
| MS.gene060401.t1 | MTR_5g026320 | 27.160 | 243 | 160 | 10 | 34 | 269 | 40 | 272 | 6.88e-12 | 67.4 |
| MS.gene060401.t1 | MTR_4g127650 | 24.275 | 276 | 160 | 10 | 31 | 276 | 27 | 283 | 7.11e-12 | 67.4 |
| MS.gene060401.t1 | MTR_3g026950 | 26.596 | 282 | 170 | 9 | 19 | 275 | 9 | 278 | 7.79e-12 | 67.4 |
| MS.gene060401.t1 | MTR_7g086000 | 25.126 | 398 | 237 | 14 | 74 | 425 | 68 | 450 | 1.40e-11 | 66.6 |
| MS.gene060401.t1 | MTR_2g105330 | 23.954 | 263 | 180 | 9 | 24 | 276 | 17 | 269 | 1.77e-11 | 66.2 |
| MS.gene060401.t1 | MTR_2g064495 | 23.420 | 269 | 189 | 8 | 18 | 276 | 8 | 269 | 2.61e-11 | 65.5 |
| MS.gene060401.t1 | MTR_3g086020 | 23.774 | 265 | 150 | 11 | 20 | 268 | 13 | 241 | 3.28e-11 | 65.1 |
| MS.gene060401.t1 | MTR_4g097090 | 22.951 | 427 | 255 | 18 | 31 | 404 | 26 | 431 | 4.24e-11 | 65.1 |
| MS.gene060401.t1 | MTR_7g084940 | 23.305 | 236 | 172 | 5 | 46 | 272 | 47 | 282 | 4.38e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060401.t1 | AT4G24510 | 40.138 | 436 | 221 | 13 | 7 | 425 | 1 | 413 | 5.84e-101 | 308 |
| MS.gene060401.t1 | AT4G13840 | 36.967 | 422 | 252 | 9 | 11 | 430 | 13 | 422 | 2.14e-91 | 283 |
| MS.gene060401.t1 | AT3G23840 | 38.018 | 434 | 243 | 12 | 1 | 430 | 1 | 412 | 9.15e-91 | 281 |
| MS.gene060401.t1 | AT4G29250 | 23.611 | 432 | 275 | 18 | 23 | 419 | 23 | 434 | 7.02e-17 | 83.2 |
| MS.gene060401.t1 | AT2G19070 | 25.424 | 295 | 190 | 10 | 19 | 291 | 11 | 297 | 6.39e-16 | 80.1 |
| MS.gene060401.t1 | AT3G48720 | 30.282 | 142 | 94 | 4 | 47 | 183 | 41 | 182 | 2.16e-12 | 68.9 |
| MS.gene060401.t1 | AT5G41040 | 25.194 | 258 | 164 | 8 | 30 | 269 | 33 | 279 | 2.91e-11 | 65.5 |
| MS.gene060401.t1 | AT5G41040 | 25.097 | 259 | 165 | 8 | 30 | 270 | 49 | 296 | 3.06e-11 | 65.5 |
| MS.gene060401.t1 | AT1G24420 | 22.105 | 285 | 171 | 9 | 20 | 281 | 13 | 269 | 4.21e-11 | 65.1 |
| MS.gene060401.t1 | AT1G24420 | 22.105 | 285 | 171 | 9 | 20 | 281 | 13 | 269 | 4.21e-11 | 65.1 |
| MS.gene060401.t1 | AT1G24430 | 24.896 | 241 | 156 | 9 | 33 | 268 | 27 | 247 | 4.63e-11 | 65.1 |
| MS.gene060401.t1 | AT5G63560 | 24.706 | 255 | 177 | 9 | 30 | 276 | 25 | 272 | 6.61e-11 | 64.3 |
Find 87 sgRNAs with CRISPR-Local
Find 241 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGGCATGTGAAGAGATTT+TGG | 0.222789 | 2.1:+53548457 | None:intergenic |
| AGGAGGAGGTGCTTCTAAAT+TGG | 0.232854 | 2.1:+53550800 | None:intergenic |
| AGGGAATGGTTGGATGAAAA+TGG | 0.238554 | 2.1:-53550477 | MS.gene060401:CDS |
| AAATTATTTCAGCTATGATA+TGG | 0.259708 | 2.1:-53548234 | MS.gene060401:CDS |
| AAAACGTTGTTGGGGAATAT+TGG | 0.260857 | 2.1:-53548372 | MS.gene060401:CDS |
| AAATCACAATTTGCCATAAT+AGG | 0.264672 | 2.1:+53547881 | None:intergenic |
| TCTTCACCTCGTGGTGTTGC+TGG | 0.285592 | 2.1:+53550750 | None:intergenic |
| CTAAGTTGGGCTCACATTCT+TGG | 0.303327 | 2.1:-53548544 | MS.gene060401:CDS |
| TCAACAAGTCCATGTAGTTT+AGG | 0.318169 | 2.1:+53550712 | None:intergenic |
| TTATTACCATCATCATCTTC+TGG | 0.337767 | 2.1:+53547815 | None:intergenic |
| TTCGATTCTGAAGCTGTTCA+AGG | 0.344410 | 2.1:-53550657 | MS.gene060401:CDS |
| GATAAACGGTCTACCGGATT+CGG | 0.346628 | 2.1:+53550554 | None:intergenic |
| CTCAACAATATGTCATTTGT+TGG | 0.348079 | 2.1:+53548115 | None:intergenic |
| ATTGGTATTGAAATTGATAT+TGG | 0.358303 | 2.1:+53548270 | None:intergenic |
| TTCAGAATCGAAGAAATAAA+TGG | 0.359775 | 2.1:+53550668 | None:intergenic |
| TAATGGGAAGAAACCTATTA+TGG | 0.366606 | 2.1:-53547894 | MS.gene060401:CDS |
| TTTACGGTGTAAAGCTTAAT+GGG | 0.367625 | 2.1:-53547910 | MS.gene060401:CDS |
| ACACCACGAGGTGAAGAAAA+AGG | 0.368645 | 2.1:-53550744 | MS.gene060401:CDS |
| GATTTGTACTCGCATGTTGC+CGG | 0.368832 | 2.1:-53550591 | MS.gene060401:CDS |
| GAAGATGATGATGGTAATAA+TGG | 0.374512 | 2.1:-53547812 | MS.gene060401:CDS |
| TTCTAGCCAACTTCACCATT+TGG | 0.381913 | 2.1:-53548302 | MS.gene060401:CDS |
| ACCACCGATGTTTCCACTGC+TGG | 0.384086 | 2.1:-53550613 | MS.gene060401:CDS |
| ACAAAATTGGATAGGAATTG+AGG | 0.389132 | 2.1:+53548424 | None:intergenic |
| GACTCTCTGTAGGACTAAGT+TGG | 0.389788 | 2.1:-53548558 | MS.gene060401:CDS |
| TTTCAAGCTTATATTTCAAC+TGG | 0.396814 | 2.1:+53547750 | None:intergenic |
| GCATCGTTGCATTTGATAAA+CGG | 0.398176 | 2.1:+53550540 | None:intergenic |
| GTTGAAATTCTTGAACTTGA+AGG | 0.399551 | 2.1:+53550780 | None:intergenic |
| ATTGGATAGGAATTGAGGTT+TGG | 0.406081 | 2.1:+53548429 | None:intergenic |
| AGCAAGTATGTGACTCCATT+TGG | 0.416229 | 2.1:+53548492 | None:intergenic |
| AATATAAGCTTGAAAGAGAA+TGG | 0.417995 | 2.1:-53547742 | MS.gene060401:CDS |
| TCAACAATATGTCATTTGTT+GGG | 0.429902 | 2.1:+53548116 | None:intergenic |
| TTAGATTTAGTGAATCTGAT+TGG | 0.430126 | 2.1:-53548052 | MS.gene060401:CDS |
| GGGTGATCAAGGAGTTGTTT+TGG | 0.437631 | 2.1:-53547849 | MS.gene060401:CDS |
| CAGTTCACGTGTTTCAAATG+TGG | 0.438540 | 2.1:-53548583 | MS.gene060401:CDS |
| AAATGGAGTCACATACTTGC+TGG | 0.439370 | 2.1:-53548490 | MS.gene060401:CDS |
| ATGATAACAGTTTCATTGCC+AGG | 0.440593 | 2.1:-53547785 | MS.gene060401:CDS |
| AATTTCAAAATACTTAGTAT+TGG | 0.449681 | 2.1:+53548252 | None:intergenic |
| ATATAAGCTTGAAAGAGAAT+GGG | 0.453313 | 2.1:-53547741 | MS.gene060401:CDS |
| AACTGGTCAAGTTCTTTCCC+TGG | 0.458794 | 2.1:+53547767 | None:intergenic |
| GGAGGAGGTGCTTCTAAATT+GGG | 0.461109 | 2.1:+53550801 | None:intergenic |
| AATTGAGGTTTGGCAAGACT+TGG | 0.469405 | 2.1:+53548439 | None:intergenic |
| CCATCATCATCTTCTGGCCC+TGG | 0.474872 | 2.1:+53547821 | None:intergenic |
| ACTCTCTGTAGGACTAAGTT+GGG | 0.477168 | 2.1:-53548557 | MS.gene060401:CDS |
| TTTATCAAATGCAACGATGC+AGG | 0.480796 | 2.1:-53550537 | MS.gene060401:CDS |
| TCTAATATATTATATTCCTC+TGG | 0.494062 | 2.1:+53548070 | None:intergenic |
| AGTTCACGTGTTTCAAATGT+GGG | 0.497596 | 2.1:-53548582 | MS.gene060401:CDS |
| AATTAATGAGAATTATGCAT+TGG | 0.501040 | 2.1:-53548020 | MS.gene060401:CDS |
| CTTGTTCATGATCATGTGCT+TGG | 0.507531 | 2.1:-53550438 | MS.gene060401:CDS |
| GGGAATGGTTGGATGAAAAT+GGG | 0.510486 | 2.1:-53550476 | MS.gene060401:CDS |
| AAATTGTGATTTACGTGGTG+TGG | 0.513306 | 2.1:-53547870 | MS.gene060401:CDS |
| AAAGTTAACATTTGTAGACT+TGG | 0.520459 | 2.1:-53547945 | MS.gene060401:CDS |
| GCATTTGATAAACGGTCTAC+CGG | 0.524003 | 2.1:+53550548 | None:intergenic |
| TGATGAAGCTCCACTATGTC+CGG | 0.527383 | 2.1:-53550692 | MS.gene060401:CDS |
| TTACGTGGTGTGGGTGATCA+AGG | 0.528901 | 2.1:-53547860 | MS.gene060401:CDS |
| CGAGTTAGAATAGCCGAATC+CGG | 0.533458 | 2.1:-53550567 | MS.gene060401:CDS |
| TGATAACAGTTTCATTGCCA+GGG | 0.539695 | 2.1:-53547784 | MS.gene060401:CDS |
| GTGATAAAACGCTGAGGGAA+TGG | 0.545873 | 2.1:-53550491 | MS.gene060401:CDS |
| AAGAGGAGACATTGGACCTA+GGG | 0.554395 | 2.1:-53548194 | MS.gene060401:CDS |
| TTCGGCTATTCTAACTCGAC+CGG | 0.556767 | 2.1:+53550572 | None:intergenic |
| AATCGAAGAAATAAATGGAC+CGG | 0.556767 | 2.1:+53550673 | None:intergenic |
| AATAAATGGACCGGACATAG+TGG | 0.562447 | 2.1:+53550682 | None:intergenic |
| AAATGTATAGCACAAATAAG+AGG | 0.564021 | 2.1:-53548211 | MS.gene060401:CDS |
| ATTCTTGAACTTGAAGGAGG+AGG | 0.570601 | 2.1:+53550786 | None:intergenic |
| TGAACACAACCTAAACTACA+TGG | 0.574702 | 2.1:-53550721 | MS.gene060401:CDS |
| GAAATTCTTGAACTTGAAGG+AGG | 0.575868 | 2.1:+53550783 | None:intergenic |
| CGATGCAGGCGTGCGAATCG+CGG | 0.576399 | 2.1:-53550523 | MS.gene060401:CDS |
| GTACATATTGTCACAACCCT+AGG | 0.578375 | 2.1:+53548178 | None:intergenic |
| TAAAACGCTGAGGGAATGGT+TGG | 0.584184 | 2.1:-53550487 | MS.gene060401:CDS |
| AATTGTGATTTACGTGGTGT+GGG | 0.587514 | 2.1:-53547869 | MS.gene060401:CDS |
| TAAGAGGAGACATTGGACCT+AGG | 0.589061 | 2.1:-53548195 | MS.gene060401:CDS |
| CGAAACAAATTTGCGTCCAG+AGG | 0.590064 | 2.1:-53548086 | MS.gene060401:CDS |
| TATAAGCTTGAAAGAGAATG+GGG | 0.591321 | 2.1:-53547740 | MS.gene060401:CDS |
| AAATTGGTGGAGAAAGGTGA+TGG | 0.594488 | 2.1:-53547995 | MS.gene060401:CDS |
| TCGTGGTGTTGCTGGAACGA+CGG | 0.595930 | 2.1:+53550758 | None:intergenic |
| GCACAAATAAGAGGAGACAT+TGG | 0.597118 | 2.1:-53548202 | MS.gene060401:CDS |
| CCAGGGCCAGAAGATGATGA+TGG | 0.599889 | 2.1:-53547821 | MS.gene060401:CDS |
| TTCACGTGTTTCAAATGTGG+GGG | 0.607666 | 2.1:-53548580 | MS.gene060401:CDS |
| GTGATGTGAAATGTGTGACT+AGG | 0.619426 | 2.1:+53548325 | None:intergenic |
| TGATGTGAAATGTGTGACTA+GGG | 0.628235 | 2.1:+53548326 | None:intergenic |
| GTTCACGTGTTTCAAATGTG+GGG | 0.631051 | 2.1:-53548581 | MS.gene060401:CDS |
| TGCGAGTACAAATCCAGCAG+TGG | 0.640512 | 2.1:+53550600 | None:intergenic |
| AAATCCAGCAGTGGAAACAT+CGG | 0.641078 | 2.1:+53550609 | None:intergenic |
| CGCATTGTGATAAAACGCTG+AGG | 0.641466 | 2.1:-53550497 | MS.gene060401:CDS |
| TCCAGCAGTGGAAACATCGG+TGG | 0.653838 | 2.1:+53550612 | None:intergenic |
| GCATTGTGATAAAACGCTGA+GGG | 0.658570 | 2.1:-53550496 | MS.gene060401:CDS |
| GTCGTTCCAGCAACACCACG+AGG | 0.723207 | 2.1:-53550756 | MS.gene060401:CDS |
| ATGGCAAATTGTGATTTACG+TGG | 0.736506 | 2.1:-53547875 | MS.gene060401:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATTATATATGATTGAT+CGG | - | chr2.1:53549862-53549881 | MS.gene060401:intron | 10.0% |
| !! | AATAATATGATGAAAAAAGT+AGG | + | chr2.1:53548182-53548201 | None:intergenic | 15.0% |
| !! | AATTTCAAAATACTTAGTAT+TGG | + | chr2.1:53550273-53550292 | None:intergenic | 15.0% |
| !! | ATATTATGTATGTTATAAGT+GGG | - | chr2.1:53548441-53548460 | MS.gene060401:CDS | 15.0% |
| !! | GTTTGATATATAAAGATAAT+AGG | + | chr2.1:53548384-53548403 | None:intergenic | 15.0% |
| !! | TGCAAATTAATATTAGAATT+TGG | + | chr2.1:53548517-53548536 | None:intergenic | 15.0% |
| !!! | AAAACAATATTTCTATTATG+TGG | + | chr2.1:53549699-53549718 | None:intergenic | 15.0% |
| !!! | AAACAATATTTCTATTATGT+GGG | + | chr2.1:53549698-53549717 | None:intergenic | 15.0% |
| !!! | AAAGATGATTTTTTAGTTTA+TGG | - | chr2.1:53550551-53550570 | MS.gene060401:CDS | 15.0% |
| !!! | ATACTCAAAATTTCTTTTTT+TGG | + | chr2.1:53549783-53549802 | None:intergenic | 15.0% |
| !!! | TACAATAGTATTAATTTGAA+GGG | + | chr2.1:53548542-53548561 | None:intergenic | 15.0% |
| !!! | TATTGATAAATTGAGATTTT+AGG | - | chr2.1:53548216-53548235 | MS.gene060401:CDS | 15.0% |
| !!! | TTACAATAGTATTAATTTGA+AGG | + | chr2.1:53548543-53548562 | None:intergenic | 15.0% |
| !!! | TTGTTTTTTTTTTCTTTTAG+AGG | - | chr2.1:53549712-53549731 | MS.gene060401:intron | 15.0% |
| !! | AAAGAATTAATTCACAAGTA+GGG | + | chr2.1:53549627-53549646 | None:intergenic | 20.0% |
| !! | AAATTATTTCAGCTATGATA+TGG | - | chr2.1:53550288-53550307 | MS.gene060401:intron | 20.0% |
| !! | AATTAATGAGAATTATGCAT+TGG | - | chr2.1:53550502-53550521 | MS.gene060401:CDS | 20.0% |
| !! | ACTAACTTATGAAATATCAA+AGG | - | chr2.1:53549147-53549166 | MS.gene060401:intron | 20.0% |
| !! | ATCTCAATTTATCAATAACA+CGG | + | chr2.1:53548213-53548232 | None:intergenic | 20.0% |
| !! | ATTTGACCTTTATACTAAAT+CGG | - | chr2.1:53548629-53548648 | MS.gene060401:intron | 20.0% |
| !! | CTAACTTATGAAATATCAAA+GGG | - | chr2.1:53549148-53549167 | MS.gene060401:intron | 20.0% |
| !! | GATATTATGTATGTTATAAG+TGG | - | chr2.1:53548440-53548459 | MS.gene060401:CDS | 20.0% |
| !! | TAAACTTTAGTTAACACTTA+TGG | - | chr2.1:53549805-53549824 | MS.gene060401:intron | 20.0% |
| !! | TAAAGCCTATTTACTTAAAT+AGG | + | chr2.1:53549501-53549520 | None:intergenic | 20.0% |
| !! | TAATACTATTGTAAGTGAAT+CGG | - | chr2.1:53548549-53548568 | MS.gene060401:CDS | 20.0% |
| !! | TATAAAGATAATAGGATAGA+AGG | + | chr2.1:53548376-53548395 | None:intergenic | 20.0% |
| !! | TATCAAAAACAAAATTGGAT+AGG | + | chr2.1:53550109-53550128 | None:intergenic | 20.0% |
| !! | TATTATGTATGTTATAAGTG+GGG | - | chr2.1:53548442-53548461 | MS.gene060401:CDS | 20.0% |
| !! | TCTAATATATTATATTCCTC+TGG | + | chr2.1:53550455-53550474 | None:intergenic | 20.0% |
| !! | TTAACACTAAGTTATAGTTT+GGG | + | chr2.1:53548329-53548348 | None:intergenic | 20.0% |
| !! | TTAATTTAACCTTACAACAT+CGG | + | chr2.1:53548488-53548507 | None:intergenic | 20.0% |
| !! | TTTAACACTAAGTTATAGTT+TGG | + | chr2.1:53548330-53548349 | None:intergenic | 20.0% |
| !! | TTTGACCTATTTAAGTAAAT+AGG | - | chr2.1:53549493-53549512 | MS.gene060401:intron | 20.0% |
| !!! | AAACCTATAATTTTTGAAGT+AGG | + | chr2.1:53549355-53549374 | None:intergenic | 20.0% |
| !!! | AACAATATTTCTATTATGTG+GGG | + | chr2.1:53549697-53549716 | None:intergenic | 20.0% |
| !!! | AATGTTTAGGGTTTAAATTT+TGG | + | chr2.1:53549068-53549087 | None:intergenic | 20.0% |
| !!! | ATAATATCTCATCCAATTTT+GGG | + | chr2.1:53548428-53548447 | None:intergenic | 20.0% |
| !!! | ATATGGTATGTATTTTTAAG+TGG | - | chr2.1:53548267-53548286 | MS.gene060401:CDS | 20.0% |
| !!! | ATCTTTTTATTAAACGAGTT+AGG | - | chr2.1:53549538-53549557 | MS.gene060401:intron | 20.0% |
| !!! | ATTGGTATTGAAATTGATAT+TGG | + | chr2.1:53550255-53550274 | None:intergenic | 20.0% |
| !!! | CAAACATAGTATTTTTAAGT+GGG | + | chr2.1:53549832-53549851 | None:intergenic | 20.0% |
| !!! | TAAAGACATTTAAGATTTGT+TGG | - | chr2.1:53548346-53548365 | MS.gene060401:CDS | 20.0% |
| !!! | TAAATTGAGATTTTAGGTTT+AGG | - | chr2.1:53548222-53548241 | MS.gene060401:CDS | 20.0% |
| !!! | TATGGTATGTATTTTTAAGT+GGG | - | chr2.1:53548268-53548287 | MS.gene060401:CDS | 20.0% |
| !!! | TCAAACATAGTATTTTTAAG+TGG | + | chr2.1:53549833-53549852 | None:intergenic | 20.0% |
| !!! | TGAAATCAACTAAAATTTTG+AGG | - | chr2.1:53549890-53549909 | MS.gene060401:intron | 20.0% |
| !!! | TTAAAACCGATTTAGTATAA+AGG | + | chr2.1:53548638-53548657 | None:intergenic | 20.0% |
| !!! | TTTCATAAGTTAGTTTAACA+AGG | + | chr2.1:53549141-53549160 | None:intergenic | 20.0% |
| !!! | TTTTCTTTTTTGATGGAAAT+GGG | + | chr2.1:53550134-53550153 | None:intergenic | 20.0% |
| ! | AAAAATTATAGGTTTGTGTC+TGG | - | chr2.1:53549360-53549379 | MS.gene060401:intron | 25.0% |
| ! | AAAGTTAACATTTGTAGACT+TGG | - | chr2.1:53550577-53550596 | MS.gene060401:CDS | 25.0% |
| ! | AAATCACAATTTGCCATAAT+AGG | + | chr2.1:53550644-53550663 | None:intergenic | 25.0% |
| ! | AAATGTATAGCACAAATAAG+AGG | - | chr2.1:53550311-53550330 | MS.gene060401:intron | 25.0% |
| ! | AAGAATTAATTCACAAGTAG+GGG | + | chr2.1:53549626-53549645 | None:intergenic | 25.0% |
| ! | AATATAAGCTTGAAAGAGAA+TGG | - | chr2.1:53550780-53550799 | MS.gene060401:CDS | 25.0% |
| ! | ACTCGTTTAATAAAAAGATC+AGG | + | chr2.1:53549537-53549556 | None:intergenic | 25.0% |
| ! | AGATAAGGAGTAAAATGTTT+AGG | + | chr2.1:53549081-53549100 | None:intergenic | 25.0% |
| ! | ATATAAGCTTGAAAGAGAAT+GGG | - | chr2.1:53550781-53550800 | MS.gene060401:CDS | 25.0% |
| ! | ATCAAAAAAGAAAACGTTGT+TGG | - | chr2.1:53550140-53550159 | MS.gene060401:intron | 25.0% |
| ! | ATTACTAAAGGAAAGACAAA+AGG | + | chr2.1:53549217-53549236 | None:intergenic | 25.0% |
| ! | ATTATGTATGTTATAAGTGG+GGG | - | chr2.1:53548443-53548462 | MS.gene060401:CDS | 25.0% |
| ! | CTAAGAAATTAAAGGAGATA+AGG | + | chr2.1:53549096-53549115 | None:intergenic | 25.0% |
| ! | GAAAGAATTAATTCACAAGT+AGG | + | chr2.1:53549628-53549647 | None:intergenic | 25.0% |
| ! | GAATTATGCATTGGAAAAAT+TGG | - | chr2.1:53550511-53550530 | MS.gene060401:CDS | 25.0% |
| ! | GATAAGGAGTAAAATGTTTA+GGG | + | chr2.1:53549080-53549099 | None:intergenic | 25.0% |
| ! | GGGATTATCAAAAACAAAAT+TGG | + | chr2.1:53550114-53550133 | None:intergenic | 25.0% |
| ! | GTTCTGCAATTATAATTGTA+TGG | - | chr2.1:53548820-53548839 | MS.gene060401:intron | 25.0% |
| ! | TAACACTAAGTTATAGTTTG+GGG | + | chr2.1:53548328-53548347 | None:intergenic | 25.0% |
| ! | TACTTAAATAGGTCAAACTA+AGG | + | chr2.1:53549490-53549509 | None:intergenic | 25.0% |
| ! | TATTATATATGATTGATCGG+TGG | - | chr2.1:53549865-53549884 | MS.gene060401:intron | 25.0% |
| ! | TCAAAAAAGAAAACGTTGTT+GGG | - | chr2.1:53550141-53550160 | MS.gene060401:intron | 25.0% |
| ! | TGACCTACTTCAAAAATTAT+AGG | - | chr2.1:53549349-53549368 | MS.gene060401:intron | 25.0% |
| ! | TTAGATTTAGTGAATCTGAT+TGG | - | chr2.1:53550470-53550489 | MS.gene060401:CDS | 25.0% |
| ! | TTCAGAATCGAAGAAATAAA+TGG | + | chr2.1:53547857-53547876 | None:intergenic | 25.0% |
| ! | TTGTTGAGCTAAGAAATTAA+AGG | + | chr2.1:53549104-53549123 | None:intergenic | 25.0% |
| ! | TTTCAAGCTTATATTTCAAC+TGG | + | chr2.1:53550775-53550794 | None:intergenic | 25.0% |
| !! | AACCTATTTAAAGTCTAACT+CGG | + | chr2.1:53549564-53549583 | None:intergenic | 25.0% |
| !! | AGGAAGAAATATTGATTGTT+GGG | - | chr2.1:53549732-53549751 | MS.gene060401:intron | 25.0% |
| !! | ATTTTGAATTGCATAACTTG+GGG | - | chr2.1:53548741-53548760 | MS.gene060401:intron | 25.0% |
| !! | CATAATATCTCATCCAATTT+TGG | + | chr2.1:53548429-53548448 | None:intergenic | 25.0% |
| !! | CATTTTGAATTGCATAACTT+GGG | - | chr2.1:53548740-53548759 | MS.gene060401:intron | 25.0% |
| !! | TCAACAATATGTCATTTGTT+GGG | + | chr2.1:53550409-53550428 | None:intergenic | 25.0% |
| !!! | AAAACTTTTTTACATGGAGA+AGG | - | chr2.1:53548698-53548717 | MS.gene060401:intron | 25.0% |
| !!! | AACAACGTTTTCTTTTTTGA+TGG | + | chr2.1:53550141-53550160 | None:intergenic | 25.0% |
| !!! | ATGGGTAAAACTTTTTTACA+TGG | - | chr2.1:53548692-53548711 | MS.gene060401:intron | 25.0% |
| !!! | ATGGTATGTATTTTTAAGTG+GGG | - | chr2.1:53548269-53548288 | MS.gene060401:CDS | 25.0% |
| !!! | CTTTTAATTTCATCACCAAA+TGG | - | chr2.1:53550015-53550034 | MS.gene060401:intron | 25.0% |
| !!! | GTTTTAAATCGGATATTGTA+AGG | + | chr2.1:53547891-53547910 | None:intergenic | 25.0% |
| !!! | GTTTTCTTTTTTGATGGAAA+TGG | + | chr2.1:53550135-53550154 | None:intergenic | 25.0% |
| !!! | TATAATTTTTGAAGTAGGTC+AGG | + | chr2.1:53549350-53549369 | None:intergenic | 25.0% |
| !!! | TATTGGTAGTTTTTACCAAA+TGG | + | chr2.1:53550238-53550257 | None:intergenic | 25.0% |
| AAAGATAATAGGATAGAAGG+TGG | + | chr2.1:53548373-53548392 | None:intergenic | 30.0% | |
| AATCGAAGAAATAAATGGAC+CGG | + | chr2.1:53547852-53547871 | None:intergenic | 30.0% | |
| ACAAAATTGGATAGGAATTG+AGG | + | chr2.1:53550101-53550120 | None:intergenic | 30.0% | |
| ATATTTCATTCAACTCCTCT+TGG | - | chr2.1:53549189-53549208 | MS.gene060401:intron | 30.0% | |
| ATTGTATGGTTCGTCTAATA+TGG | - | chr2.1:53548834-53548853 | MS.gene060401:intron | 30.0% | |
| ATTGTTAGTTAGTGGAAAGA+AGG | - | chr2.1:53548584-53548603 | MS.gene060401:intron | 30.0% | |
| CAAAAAAGAAAACGTTGTTG+GGG | - | chr2.1:53550142-53550161 | MS.gene060401:intron | 30.0% | |
| CCAAGTTATGCAATTCAAAA+TGG | + | chr2.1:53548742-53548761 | None:intergenic | 30.0% | |
| CTTTAAATAGGTTATGTCGT+AGG | - | chr2.1:53549571-53549590 | MS.gene060401:intron | 30.0% | |
| GCTCTTAACACATATATAAG+TGG | + | chr2.1:53548406-53548425 | None:intergenic | 30.0% | |
| GTCTCACATTAGATGTTATA+TGG | - | chr2.1:53548250-53548269 | MS.gene060401:CDS | 30.0% | |
| GTCTGATCTGACATTTATAA+AGG | - | chr2.1:53549413-53549432 | MS.gene060401:intron | 30.0% | |
| GTTGAAATTCTTGAACTTGA+AGG | + | chr2.1:53547745-53547764 | None:intergenic | 30.0% | |
| TAACATGCATTGTTAGTTAG+TGG | - | chr2.1:53548576-53548595 | MS.gene060401:CDS | 30.0% | |
| TAATGGGAAGAAACCTATTA+TGG | - | chr2.1:53550628-53550647 | MS.gene060401:CDS | 30.0% | |
| TATAAGCTTGAAAGAGAATG+GGG | - | chr2.1:53550782-53550801 | MS.gene060401:CDS | 30.0% | |
| TCTTTCATAGCAGAAAACTT+GGG | - | chr2.1:53549642-53549661 | MS.gene060401:intron | 30.0% | |
| TTATGCATTGGAAAAATTGG+TGG | - | chr2.1:53550514-53550533 | MS.gene060401:CDS | 30.0% | |
| TTATTACCATCATCATCTTC+TGG | + | chr2.1:53550710-53550729 | None:intergenic | 30.0% | |
| TTCTTTCATAGCAGAAAACT+TGG | - | chr2.1:53549641-53549660 | MS.gene060401:intron | 30.0% | |
| TTTACGGTGTAAAGCTTAAT+GGG | - | chr2.1:53550612-53550631 | MS.gene060401:CDS | 30.0% | |
| ! | CCATTTTGAATTGCATAACT+TGG | - | chr2.1:53548739-53548758 | MS.gene060401:intron | 30.0% |
| ! | CTCAACAATATGTCATTTGT+TGG | + | chr2.1:53550410-53550429 | None:intergenic | 30.0% |
| ! | GAATTTTACAAAACCGAAGA+TGG | + | chr2.1:53548303-53548322 | None:intergenic | 30.0% |
| ! | GAGGAAGAAATATTGATTGT+TGG | - | chr2.1:53549731-53549750 | MS.gene060401:intron | 30.0% |
| ! | TATCAAAGGGCATAAACATA+AGG | - | chr2.1:53549161-53549180 | MS.gene060401:intron | 30.0% |
| ! | TATTTCTATTATGTGGGGTT+CGG | + | chr2.1:53549692-53549711 | None:intergenic | 30.0% |
| ! | TCATTTTCAGCACATTTAGA+GGG | + | chr2.1:53550383-53550402 | None:intergenic | 30.0% |
| ! | TTCATTTTCAGCACATTTAG+AGG | + | chr2.1:53550384-53550403 | None:intergenic | 30.0% |
| ! | TTTTACGGTGTAAAGCTTAA+TGG | - | chr2.1:53550611-53550630 | MS.gene060401:CDS | 30.0% |
| ! | TTTTTACCAAATGGTGAAGT+TGG | + | chr2.1:53550229-53550248 | None:intergenic | 30.0% |
| !! | GAAGATGATGATGGTAATAA+TGG | - | chr2.1:53550710-53550729 | MS.gene060401:CDS | 30.0% |
| !! | TTGGAAGAAGCTGATTTTTA+CGG | - | chr2.1:53550596-53550615 | MS.gene060401:CDS | 30.0% |
| !!! | AAACATCGGTGGTTTTAAAT+CGG | + | chr2.1:53547902-53547921 | None:intergenic | 30.0% |
| !!! | TGGTATGTATTTTTAAGTGG+GGG | - | chr2.1:53548270-53548289 | MS.gene060401:CDS | 30.0% |
| AAAACGTTGTTGGGGAATAT+TGG | - | chr2.1:53550150-53550169 | MS.gene060401:intron | 35.0% | |
| AAAAGCCTATTAAGTCCATC+AGG | + | chr2.1:53549460-53549479 | None:intergenic | 35.0% | |
| AAAGGAGAAAAAGCAAGATC+AGG | + | chr2.1:53548111-53548130 | None:intergenic | 35.0% | |
| AAATTGTGATTTACGTGGTG+TGG | - | chr2.1:53550652-53550671 | MS.gene060401:CDS | 35.0% | |
| AATTGTGATTTACGTGGTGT+GGG | - | chr2.1:53550653-53550672 | MS.gene060401:CDS | 35.0% | |
| AGAATTGCTACCTGTGAATA+AGG | - | chr2.1:53549282-53549301 | MS.gene060401:intron | 35.0% | |
| AGTGGAAAGAAGGTTTACAT+TGG | - | chr2.1:53548594-53548613 | MS.gene060401:intron | 35.0% | |
| AGTTCACGTGTTTCAAATGT+GGG | - | chr2.1:53549940-53549959 | MS.gene060401:intron | 35.0% | |
| ATGAGATTGTGATAAGCAAG+AGG | + | chr2.1:53549245-53549264 | None:intergenic | 35.0% | |
| ATGATAACAGTTTCATTGCC+AGG | - | chr2.1:53550737-53550756 | MS.gene060401:CDS | 35.0% | |
| ATGGCAAATTGTGATTTACG+TGG | - | chr2.1:53550647-53550666 | MS.gene060401:CDS | 35.0% | |
| ATGTGTTAAGAGCCCAAAAT+TGG | - | chr2.1:53548413-53548432 | MS.gene060401:CDS | 35.0% | |
| ATTGGATAGGAATTGAGGTT+TGG | + | chr2.1:53550096-53550115 | None:intergenic | 35.0% | |
| CATTGTGATATGCTGACAAT+GGG | - | chr2.1:53548674-53548693 | MS.gene060401:intron | 35.0% | |
| GAAATTCTTGAACTTGAAGG+AGG | + | chr2.1:53547742-53547761 | None:intergenic | 35.0% | |
| GAATTGCTACCTGTGAATAA+GGG | - | chr2.1:53549283-53549302 | MS.gene060401:intron | 35.0% | |
| GCATCGTTGCATTTGATAAA+CGG | + | chr2.1:53547985-53548004 | None:intergenic | 35.0% | |
| GTGGAAAGAAGGTTTACATT+GGG | - | chr2.1:53548595-53548614 | MS.gene060401:intron | 35.0% | |
| TAAAGGTCTGAAAGCTTGAT+AGG | - | chr2.1:53549430-53549449 | MS.gene060401:intron | 35.0% | |
| TCAACAAGTCCATGTAGTTT+AGG | + | chr2.1:53547813-53547832 | None:intergenic | 35.0% | |
| TCATTGTGATATGCTGACAA+TGG | - | chr2.1:53548673-53548692 | MS.gene060401:intron | 35.0% | |
| TGAACACAACCTAAACTACA+TGG | - | chr2.1:53547801-53547820 | MS.gene060401:CDS | 35.0% | |
| TGAGATTGTGATAAGCAAGA+GGG | + | chr2.1:53549244-53549263 | None:intergenic | 35.0% | |
| TGATAACAGTTTCATTGCCA+GGG | - | chr2.1:53550738-53550757 | MS.gene060401:CDS | 35.0% | |
| TGATGTGAAATGTGTGACTA+GGG | + | chr2.1:53550199-53550218 | None:intergenic | 35.0% | |
| TGGAAAGAAGGTTTACATTG+GGG | - | chr2.1:53548596-53548615 | MS.gene060401:intron | 35.0% | |
| TTGGAAAAATTGGTGGAGAA+AGG | - | chr2.1:53550521-53550540 | MS.gene060401:CDS | 35.0% | |
| TTTATCAAATGCAACGATGC+AGG | - | chr2.1:53547985-53548004 | MS.gene060401:CDS | 35.0% | |
| ! | TCGTCATAGACTCATTTTCA+AGG | - | chr2.1:53549391-53549410 | MS.gene060401:intron | 35.0% |
| !!! | CATGTGAAGAGATTTTGGTT+GGG | + | chr2.1:53550063-53550082 | None:intergenic | 35.0% |
| !!! | TGATCTTGCTTTTTCTCCTT+TGG | - | chr2.1:53548110-53548129 | MS.gene060401:CDS | 35.0% |
| !!! | TTCTCCTTTGGTTTTTGTCA+AGG | - | chr2.1:53548122-53548141 | MS.gene060401:CDS | 35.0% |
| AAATCCAGCAGTGGAAACAT+CGG | + | chr2.1:53547916-53547935 | None:intergenic | 40.0% | |
| AAATGGAGTCACATACTTGC+TGG | - | chr2.1:53550032-53550051 | MS.gene060401:intron | 40.0% | |
| AAATTGGTGGAGAAAGGTGA+TGG | - | chr2.1:53550527-53550546 | MS.gene060401:CDS | 40.0% | |
| AATAAATGGACCGGACATAG+TGG | + | chr2.1:53547843-53547862 | None:intergenic | 40.0% | |
| AATTGAGGTTTGGCAAGACT+TGG | + | chr2.1:53550086-53550105 | None:intergenic | 40.0% | |
| ACTCTCTGTAGGACTAAGTT+GGG | - | chr2.1:53549965-53549984 | MS.gene060401:intron | 40.0% | |
| AGCAAGTATGTGACTCCATT+TGG | + | chr2.1:53550033-53550052 | None:intergenic | 40.0% | |
| AGGGAATGGTTGGATGAAAA+TGG | - | chr2.1:53548045-53548064 | MS.gene060401:CDS | 40.0% | |
| ATTCTTGAACTTGAAGGAGG+AGG | + | chr2.1:53547739-53547758 | None:intergenic | 40.0% | |
| CAGTTCACGTGTTTCAAATG+TGG | - | chr2.1:53549939-53549958 | MS.gene060401:intron | 40.0% | |
| CCTTACAAACCGATGTTGTA+AGG | - | chr2.1:53548476-53548495 | MS.gene060401:CDS | 40.0% | |
| CGTACCTTGACAAAAACCAA+AGG | + | chr2.1:53548129-53548148 | None:intergenic | 40.0% | |
| CTATTTCCACCCTTATTCAC+AGG | + | chr2.1:53549295-53549314 | None:intergenic | 40.0% | |
| CTGTGAATAAGGGTGGAAAT+AGG | - | chr2.1:53549293-53549312 | MS.gene060401:intron | 40.0% | |
| CTTGTTCATGATCATGTGCT+TGG | - | chr2.1:53548084-53548103 | MS.gene060401:CDS | 40.0% | |
| GAAAGACAAAAGGTTCCAAG+AGG | + | chr2.1:53549207-53549226 | None:intergenic | 40.0% | |
| GCACAAATAAGAGGAGACAT+TGG | - | chr2.1:53550320-53550339 | MS.gene060401:intron | 40.0% | |
| GCATTGTGATAAAACGCTGA+GGG | - | chr2.1:53548026-53548045 | MS.gene060401:CDS | 40.0% | |
| GCATTTGATAAACGGTCTAC+CGG | + | chr2.1:53547977-53547996 | None:intergenic | 40.0% | |
| GGCCGAGTTAGACTTTAAAT+AGG | - | chr2.1:53549559-53549578 | MS.gene060401:intron | 40.0% | |
| GGGAATGGTTGGATGAAAAT+GGG | - | chr2.1:53548046-53548065 | MS.gene060401:CDS | 40.0% | |
| GTACATATTGTCACAACCCT+AGG | + | chr2.1:53550347-53550366 | None:intergenic | 40.0% | |
| GTGATGTGAAATGTGTGACT+AGG | + | chr2.1:53550200-53550219 | None:intergenic | 40.0% | |
| GTTCACGTGTTTCAAATGTG+GGG | - | chr2.1:53549941-53549960 | MS.gene060401:intron | 40.0% | |
| TTCACGTGTTTCAAATGTGG+GGG | - | chr2.1:53549942-53549961 | MS.gene060401:intron | 40.0% | |
| TTCTAGCCAACTTCACCATT+TGG | - | chr2.1:53550220-53550239 | MS.gene060401:intron | 40.0% | |
| ! | CAAGAGGGCATGATTACTAA+AGG | + | chr2.1:53549229-53549248 | None:intergenic | 40.0% |
| ! | CTTGGCATGTGAAGAGATTT+TGG | + | chr2.1:53550068-53550087 | None:intergenic | 40.0% |
| !! | CCTTACAACATCGGTTTGTA+AGG | + | chr2.1:53548479-53548498 | None:intergenic | 40.0% |
| !! | GCATGTGAAGAGATTTTGGT+TGG | + | chr2.1:53550064-53550083 | None:intergenic | 40.0% |
| !! | TTCGATTCTGAAGCTGTTCA+AGG | - | chr2.1:53547865-53547884 | MS.gene060401:CDS | 40.0% |
| !!! | GAGTTGTTTTGGTTCTTCCA+GGG | - | chr2.1:53550684-53550703 | MS.gene060401:CDS | 40.0% |
| !!! | TTTTGAAGTAGGTCAGGCTT+AGG | + | chr2.1:53549344-53549363 | None:intergenic | 40.0% |
| AACTGGTCAAGTTCTTTCCC+TGG | + | chr2.1:53550758-53550777 | None:intergenic | 45.0% | |
| AAGAGGAGACATTGGACCTA+GGG | - | chr2.1:53550328-53550347 | MS.gene060401:intron | 45.0% | |
| ACACCACGAGGTGAAGAAAA+AGG | - | chr2.1:53547778-53547797 | MS.gene060401:CDS | 45.0% | |
| CGAAACAAATTTGCGTCCAG+AGG | - | chr2.1:53550436-53550455 | MS.gene060401:CDS | 45.0% | |
| CGAGTTAGAATAGCCGAATC+CGG | - | chr2.1:53547955-53547974 | MS.gene060401:CDS | 45.0% | |
| CGCATTGTGATAAAACGCTG+AGG | - | chr2.1:53548025-53548044 | MS.gene060401:CDS | 45.0% | |
| GACTCTCTGTAGGACTAAGT+TGG | - | chr2.1:53549964-53549983 | MS.gene060401:intron | 45.0% | |
| GATAAACGGTCTACCGGATT+CGG | + | chr2.1:53547971-53547990 | None:intergenic | 45.0% | |
| GATTTGTACTCGCATGTTGC+CGG | - | chr2.1:53547931-53547950 | MS.gene060401:CDS | 45.0% | |
| GTGATAAAACGCTGAGGGAA+TGG | - | chr2.1:53548031-53548050 | MS.gene060401:CDS | 45.0% | |
| TAAAACGCTGAGGGAATGGT+TGG | - | chr2.1:53548035-53548054 | MS.gene060401:CDS | 45.0% | |
| TAAGAGGAGACATTGGACCT+AGG | - | chr2.1:53550327-53550346 | MS.gene060401:intron | 45.0% | |
| TGATGAAGCTCCACTATGTC+CGG | - | chr2.1:53547830-53547849 | MS.gene060401:CDS | 45.0% | |
| TGGGGGACAATTTCCATCTT+CGG | - | chr2.1:53548287-53548306 | MS.gene060401:CDS | 45.0% | |
| TTCGGCTATTCTAACTCGAC+CGG | + | chr2.1:53547953-53547972 | None:intergenic | 45.0% | |
| TTGCTACCTGTGAATAAGGG+TGG | - | chr2.1:53549286-53549305 | MS.gene060401:intron | 45.0% | |
| ! | CTAAGTTGGGCTCACATTCT+TGG | - | chr2.1:53549978-53549997 | MS.gene060401:intron | 45.0% |
| ! | GGGTGATCAAGGAGTTGTTT+TGG | - | chr2.1:53550673-53550692 | MS.gene060401:CDS | 45.0% |
| ! | TCACCTTTTTCTTCACCTCG+TGG | + | chr2.1:53547784-53547803 | None:intergenic | 45.0% |
| !! | AGGAGGAGGTGCTTCTAAAT+TGG | + | chr2.1:53547725-53547744 | None:intergenic | 45.0% |
| !! | GGAGGAGGTGCTTCTAAATT+GGG | + | chr2.1:53547724-53547743 | None:intergenic | 45.0% |
| !!! | GGAGTTGTTTTGGTTCTTCC+AGG | - | chr2.1:53550683-53550702 | MS.gene060401:CDS | 45.0% |
| !!! | ATAATTAATTTGTAAAAAAA+AGG | + | chr2.1:53548800-53548819 | None:intergenic | 5.0% |
| AAATGTGGGGGACTCTCTGT+AGG | - | chr2.1:53549954-53549973 | MS.gene060401:intron | 50.0% | |
| AGGGTGGAAATAGGCTAGAC+CGG | - | chr2.1:53549302-53549321 | MS.gene060401:intron | 50.0% | |
| GCCTATTAAGTCCATCAGGC+TGG | + | chr2.1:53549456-53549475 | None:intergenic | 50.0% | |
| GGCTTAGGCCTTACAAAGTC+CGG | + | chr2.1:53549329-53549348 | None:intergenic | 50.0% | |
| TGCGAGTACAAATCCAGCAG+TGG | + | chr2.1:53547925-53547944 | None:intergenic | 50.0% | |
| TTACGTGGTGTGGGTGATCA+AGG | - | chr2.1:53550662-53550681 | MS.gene060401:CDS | 50.0% | |
| !! | GCCAGCCTGATGGACTTAAT+AGG | - | chr2.1:53549452-53549471 | MS.gene060401:intron | 50.0% |
| ACCACCGATGTTTCCACTGC+TGG | - | chr2.1:53547909-53547928 | MS.gene060401:CDS | 55.0% | |
| AGCTTGATAGGCCAGCCTGA+TGG | - | chr2.1:53549442-53549461 | MS.gene060401:intron | 55.0% | |
| CCAGGGCCAGAAGATGATGA+TGG | - | chr2.1:53550701-53550720 | MS.gene060401:CDS | 55.0% | |
| CCATCATCATCTTCTGGCCC+TGG | + | chr2.1:53550704-53550723 | None:intergenic | 55.0% | |
| GGGTGGAAATAGGCTAGACC+GGG | - | chr2.1:53549303-53549322 | MS.gene060401:intron | 55.0% | |
| GTTATGTCGTAGGCCCCTGT+CGG | - | chr2.1:53549581-53549600 | MS.gene060401:intron | 55.0% | |
| TCCAGCAGTGGAAACATCGG+TGG | + | chr2.1:53547913-53547932 | None:intergenic | 55.0% | |
| TTATGTCGTAGGCCCCTGTC+GGG | - | chr2.1:53549582-53549601 | MS.gene060401:intron | 55.0% | |
| !! | TCGTGGTGTTGCTGGAACGA+CGG | + | chr2.1:53547767-53547786 | None:intergenic | 55.0% |
| !! | TCTTCACCTCGTGGTGTTGC+TGG | + | chr2.1:53547775-53547794 | None:intergenic | 55.0% |
| AGACCGGGCCGGACTTTGTA+AGG | - | chr2.1:53549318-53549337 | MS.gene060401:intron | 60.0% | |
| AGGCCTTACAAAGTCCGGCC+CGG | + | chr2.1:53549324-53549343 | None:intergenic | 60.0% | |
| GGAAATAGGCTAGACCGGGC+CGG | - | chr2.1:53549307-53549326 | MS.gene060401:intron | 60.0% | |
| GTCGTTCCAGCAACACCACG+AGG | - | chr2.1:53547766-53547785 | MS.gene060401:CDS | 60.0% | |
| AACATGCCTGACCGCCCGAC+AGG | + | chr2.1:53549599-53549618 | None:intergenic | 65.0% | |
| ACATGCCTGACCGCCCGACA+GGG | + | chr2.1:53549598-53549617 | None:intergenic | 65.0% | |
| CGATGCAGGCGTGCGAATCG+CGG | - | chr2.1:53547999-53548018 | MS.gene060401:CDS | 65.0% | |
| CATGCCTGACCGCCCGACAG+GGG | + | chr2.1:53549597-53549616 | None:intergenic | 70.0% | |
| TGTCGTAGGCCCCTGTCGGG+CGG | - | chr2.1:53549585-53549604 | MS.gene060401:intron | 70.0% | |
| TAGGCCCCTGTCGGGCGGTC+AGG | - | chr2.1:53549590-53549609 | MS.gene060401:intron | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 53547709 | 53550835 | 53547709 | ID=MS.gene060401 |
| chr2.1 | mRNA | 53547709 | 53550835 | 53547709 | ID=MS.gene060401.t1;Parent=MS.gene060401 |
| chr2.1 | exon | 53550401 | 53550835 | 53550401 | ID=MS.gene060401.t1.exon1;Parent=MS.gene060401.t1 |
| chr2.1 | CDS | 53550401 | 53550835 | 53550401 | ID=cds.MS.gene060401.t1;Parent=MS.gene060401.t1 |
| chr2.1 | exon | 53547709 | 53548602 | 53547709 | ID=MS.gene060401.t1.exon2;Parent=MS.gene060401.t1 |
| chr2.1 | CDS | 53547709 | 53548602 | 53547709 | ID=cds.MS.gene060401.t1;Parent=MS.gene060401.t1 |
| Gene Sequence |
| Protein sequence |
