Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056151.t1 | XP_013448450.1 | 87.3 | 63 | 8 | 0 | 1 | 63 | 20 | 82 | 9.00E-20 | 105.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056151.t1 | O03982 | 45.6 | 57 | 30 | 1 | 1 | 56 | 20 | 76 | 5.7e-07 | 54.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056151.t1 | A0A072TYH4 | 87.3 | 63 | 8 | 0 | 1 | 63 | 20 | 82 | 6.5e-20 | 105.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050251 | MS.gene056151 | 0.815015 | 1.15E-51 | -1.69E-46 |
| MS.gene056150 | MS.gene056151 | 0.889158 | 3.11E-73 | -1.69E-46 |
| MS.gene056151 | MS.gene26035 | 0.801335 | 9.45E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056151.t1 | MTR_7g451210 | 87.302 | 63 | 8 | 0 | 1 | 63 | 20 | 82 | 8.86e-35 | 114 |
| MS.gene056151.t1 | MTR_7g451200 | 82.540 | 63 | 11 | 0 | 1 | 63 | 20 | 82 | 9.99e-34 | 111 |
| MS.gene056151.t1 | MTR_1173s0010 | 82.540 | 63 | 11 | 0 | 1 | 63 | 20 | 82 | 1.43e-33 | 110 |
| MS.gene056151.t1 | MTR_3g435000 | 85.714 | 63 | 9 | 0 | 1 | 63 | 24 | 86 | 7.65e-33 | 108 |
| MS.gene056151.t1 | MTR_3g434960 | 79.365 | 63 | 13 | 0 | 1 | 63 | 20 | 82 | 2.86e-32 | 107 |
| MS.gene056151.t1 | MTR_3g434890 | 79.365 | 63 | 13 | 0 | 1 | 63 | 20 | 82 | 3.33e-32 | 107 |
| MS.gene056151.t1 | MTR_6g049260 | 75.806 | 62 | 15 | 0 | 1 | 62 | 20 | 81 | 2.19e-27 | 95.1 |
| MS.gene056151.t1 | MTR_3g434920 | 78.182 | 55 | 12 | 0 | 9 | 63 | 2 | 56 | 2.91e-27 | 94.0 |
| MS.gene056151.t1 | MTR_1g099800 | 64.000 | 50 | 18 | 0 | 1 | 50 | 20 | 69 | 7.14e-19 | 74.3 |
| MS.gene056151.t1 | MTR_1g099810 | 60.000 | 50 | 20 | 0 | 1 | 50 | 1 | 50 | 2.98e-18 | 72.4 |
| MS.gene056151.t1 | MTR_1g099810 | 60.000 | 50 | 20 | 0 | 1 | 50 | 20 | 69 | 4.75e-18 | 72.4 |
| MS.gene056151.t1 | MTR_6g049320 | 62.000 | 50 | 19 | 0 | 1 | 50 | 20 | 69 | 7.85e-18 | 71.2 |
| MS.gene056151.t1 | MTR_6g049280 | 60.000 | 50 | 20 | 0 | 1 | 50 | 20 | 69 | 1.29e-17 | 70.9 |
| MS.gene056151.t1 | MTR_6g049320 | 62.000 | 50 | 19 | 0 | 1 | 50 | 50 | 99 | 1.43e-17 | 71.2 |
| MS.gene056151.t1 | MTR_8g098915 | 56.604 | 53 | 23 | 0 | 1 | 53 | 1 | 53 | 1.89e-17 | 69.7 |
| MS.gene056151.t1 | MTR_8g098915 | 56.604 | 53 | 23 | 0 | 1 | 53 | 20 | 72 | 2.76e-17 | 70.1 |
| MS.gene056151.t1 | MTR_6g049280 | 60.000 | 50 | 20 | 0 | 1 | 50 | 50 | 99 | 3.48e-17 | 70.5 |
| MS.gene056151.t1 | MTR_6g051680 | 56.000 | 50 | 22 | 0 | 1 | 50 | 20 | 69 | 2.02e-16 | 67.4 |
| MS.gene056151.t1 | MTR_6g051690 | 56.000 | 50 | 22 | 0 | 1 | 50 | 20 | 69 | 4.08e-16 | 67.0 |
| MS.gene056151.t1 | MTR_1g099640 | 53.061 | 49 | 23 | 0 | 1 | 49 | 20 | 68 | 8.32e-15 | 63.5 |
| MS.gene056151.t1 | MTR_1g099815 | 55.769 | 52 | 22 | 1 | 1 | 52 | 20 | 70 | 3.78e-13 | 59.7 |
| MS.gene056151.t1 | MTR_1g102240 | 55.769 | 52 | 22 | 1 | 1 | 52 | 20 | 70 | 1.31e-12 | 58.2 |
| MS.gene056151.t1 | MTR_8g028615 | 48.936 | 47 | 24 | 0 | 3 | 49 | 22 | 68 | 2.56e-12 | 57.8 |
| MS.gene056151.t1 | MTR_7g079110 | 44.898 | 49 | 27 | 0 | 1 | 49 | 20 | 68 | 3.12e-12 | 58.2 |
| MS.gene056151.t1 | MTR_0795s0020 | 52.941 | 51 | 18 | 1 | 1 | 51 | 20 | 64 | 6.79e-12 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGACAGCATCCAGCCTTTC+AGG | 0.250035 | 7.4:+61739019 | MS.gene056151:CDS |
| GAGAAGAGATTAACATTATC+GGG | 0.360117 | 7.4:+61739598 | MS.gene056151:CDS |
| AGAGAAGAGATTAACATTAT+CGG | 0.398276 | 7.4:+61739597 | MS.gene056151:CDS |
| TCAATATGTACCTGAAAGGC+TGG | 0.458728 | 7.4:-61739029 | None:intergenic |
| CAAACTGAAGTAGTTACGAT+TGG | 0.578605 | 7.4:+61739667 | MS.gene056151:CDS |
| TACGATTGGACCTCTAAAAG+AGG | 0.583295 | 7.4:+61739681 | MS.gene056151:CDS |
| AAATTCAATATGTACCTGAA+AGG | 0.592630 | 7.4:-61739033 | None:intergenic |
| AGAAGAGATTAACATTATCG+GGG | 0.638995 | 7.4:+61739599 | MS.gene056151:CDS |
| GAAGAGATTAACATTATCGG+GGG | 0.676086 | 7.4:+61739600 | MS.gene056151:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTATTTTTATTTTGC+AGG | + | chr7.4:61739547-61739566 | MS.gene056151:intron | 10.0% |
| !!! | TAACTTAAATTAATTTGATA+AGG | + | chr7.4:61739365-61739384 | MS.gene056151:intron | 10.0% |
| !!! | TTTTTTATTTTTATTTTGCA+GGG | + | chr7.4:61739548-61739567 | MS.gene056151:intron | 10.0% |
| !! | AAAATTAAAATCAATCACAA+TGG | - | chr7.4:61739326-61739345 | None:intergenic | 15.0% |
| !! | ATAGTATGTAAAGTTTATTT+CGG | - | chr7.4:61739174-61739193 | None:intergenic | 15.0% |
| !!! | TTTTTATTTTTATTTTGCAG+GGG | + | chr7.4:61739549-61739568 | MS.gene056151:intron | 15.0% |
| !! | AATGAATCTTTCATGTTATA+TGG | - | chr7.4:61739530-61739549 | None:intergenic | 20.0% |
| !! | ATTTATTGTATTCAAGAGTT+TGG | + | chr7.4:61739192-61739211 | MS.gene056151:intron | 20.0% |
| !! | TAGTATGTAAAGTTTATTTC+GGG | - | chr7.4:61739173-61739192 | None:intergenic | 20.0% |
| ! | AAATTCAATATGTACCTGAA+AGG | - | chr7.4:61739036-61739055 | None:intergenic | 25.0% |
| ! | AGAGAAGAGATTAACATTAT+CGG | + | chr7.4:61739597-61739616 | MS.gene056151:CDS | 25.0% |
| !! | TAGATTTTATTCTCAAACGA+CGG | - | chr7.4:61739472-61739491 | None:intergenic | 25.0% |
| !!! | GTGTCTTATTCATTTTTTGA+AGG | - | chr7.4:61739138-61739157 | None:intergenic | 25.0% |
| AGAAGAGATTAACATTATCG+GGG | + | chr7.4:61739599-61739618 | MS.gene056151:CDS | 30.0% | |
| AGTACTACTACTATGTAATG+TGG | + | chr7.4:61739065-61739084 | MS.gene056151:intron | 30.0% | |
| GAGAAGAGATTAACATTATC+GGG | + | chr7.4:61739598-61739617 | MS.gene056151:CDS | 30.0% | |
| AATGGTATCATAAGAGCAGA+TGG | - | chr7.4:61739308-61739327 | None:intergenic | 35.0% | |
| AGAAATTCACACATTAAGCG+AGG | + | chr7.4:61739218-61739237 | MS.gene056151:intron | 35.0% | |
| CAAACTGAAGTAGTTACGAT+TGG | + | chr7.4:61739667-61739686 | MS.gene056151:CDS | 35.0% | |
| GAAGAGATTAACATTATCGG+GGG | + | chr7.4:61739600-61739619 | MS.gene056151:CDS | 35.0% | |
| ! | TCTTTCTTCTCCTCTTTTAG+AGG | - | chr7.4:61739694-61739713 | None:intergenic | 35.0% |
| GGGGGATATCGATACAATAA+AGG | + | chr7.4:61739618-61739637 | MS.gene056151:CDS | 40.0% | |
| TCAATATGTACCTGAAAGGC+TGG | - | chr7.4:61739032-61739051 | None:intergenic | 40.0% | |
| ! | TACGATTGGACCTCTAAAAG+AGG | + | chr7.4:61739681-61739700 | MS.gene056151:CDS | 40.0% |
| CGAGGACAAGAGAGATATAG+CGG | + | chr7.4:61739236-61739255 | MS.gene056151:intron | 45.0% | |
| GAGGACAAGAGAGATATAGC+GGG | + | chr7.4:61739237-61739256 | MS.gene056151:intron | 45.0% | |
| AAGACAGCATCCAGCCTTTC+AGG | + | chr7.4:61739019-61739038 | MS.gene056151:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 61739016 | 61739732 | 61739016 | ID=MS.gene056151 |
| chr7.4 | mRNA | 61739016 | 61739732 | 61739016 | ID=MS.gene056151.t1;Parent=MS.gene056151 |
| chr7.4 | exon | 61739016 | 61739040 | 61739016 | ID=MS.gene056151.t1.exon1;Parent=MS.gene056151.t1 |
| chr7.4 | CDS | 61739016 | 61739040 | 61739016 | ID=cds.MS.gene056151.t1;Parent=MS.gene056151.t1 |
| chr7.4 | exon | 61739569 | 61739732 | 61739569 | ID=MS.gene056151.t1.exon2;Parent=MS.gene056151.t1 |
| chr7.4 | CDS | 61739569 | 61739732 | 61739569 | ID=cds.MS.gene056151.t1;Parent=MS.gene056151.t1 |
| Gene Sequence |
| Protein sequence |
