Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26035.t1 | XP_013448450.1 | 90.7 | 107 | 10 | 0 | 1 | 107 | 3 | 109 | 4.80E-45 | 190.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26035.t1 | O03982 | 50.0 | 74 | 36 | 1 | 1 | 73 | 3 | 76 | 1.7e-14 | 80.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26035.t1 | A0A072TYH4 | 90.7 | 107 | 10 | 0 | 1 | 107 | 3 | 109 | 3.4e-45 | 190.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050251 | MS.gene26035 | 0.947961 | 2.42E-106 | -1.69E-46 |
| MS.gene056150 | MS.gene26035 | 0.813367 | 2.65E-51 | -1.69E-46 |
| MS.gene056151 | MS.gene26035 | 0.801335 | 9.45E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26035.t1 | MTR_7g451210 | 90.654 | 107 | 10 | 0 | 1 | 107 | 3 | 109 | 2.94e-68 | 200 |
| MS.gene26035.t1 | MTR_1173s0010 | 84.259 | 108 | 17 | 0 | 1 | 108 | 3 | 110 | 1.20e-64 | 191 |
| MS.gene26035.t1 | MTR_3g434890 | 83.333 | 108 | 18 | 0 | 1 | 108 | 3 | 110 | 4.23e-64 | 189 |
| MS.gene26035.t1 | MTR_7g451200 | 84.259 | 108 | 17 | 0 | 1 | 108 | 3 | 110 | 1.21e-63 | 188 |
| MS.gene26035.t1 | MTR_3g434960 | 82.407 | 108 | 19 | 0 | 1 | 108 | 3 | 110 | 2.73e-63 | 187 |
| MS.gene26035.t1 | MTR_3g435000 | 87.879 | 99 | 12 | 0 | 4 | 102 | 10 | 108 | 2.53e-59 | 177 |
| MS.gene26035.t1 | MTR_6g049260 | 75.926 | 108 | 26 | 0 | 1 | 108 | 3 | 110 | 3.68e-54 | 164 |
| MS.gene26035.t1 | MTR_3g434920 | 79.518 | 83 | 17 | 0 | 26 | 108 | 2 | 84 | 3.00e-46 | 144 |
| MS.gene26035.t1 | MTR_6g049320 | 56.731 | 104 | 43 | 1 | 1 | 102 | 3 | 106 | 7.22e-33 | 111 |
| MS.gene26035.t1 | MTR_6g049320 | 56.731 | 104 | 43 | 1 | 1 | 102 | 33 | 136 | 2.18e-32 | 111 |
| MS.gene26035.t1 | MTR_6g049280 | 54.808 | 104 | 45 | 1 | 1 | 102 | 3 | 106 | 2.96e-32 | 109 |
| MS.gene26035.t1 | MTR_6g049280 | 54.808 | 104 | 45 | 1 | 1 | 102 | 33 | 136 | 1.55e-31 | 108 |
| MS.gene26035.t1 | MTR_6g051680 | 62.687 | 67 | 25 | 0 | 1 | 67 | 3 | 69 | 1.22e-27 | 97.8 |
| MS.gene26035.t1 | MTR_6g051690 | 62.687 | 67 | 25 | 0 | 1 | 67 | 3 | 69 | 1.19e-26 | 95.9 |
| MS.gene26035.t1 | MTR_1g099800 | 64.179 | 67 | 24 | 0 | 1 | 67 | 3 | 69 | 2.16e-26 | 95.5 |
| MS.gene26035.t1 | MTR_1g099810 | 62.687 | 67 | 25 | 0 | 1 | 67 | 3 | 69 | 1.42e-25 | 93.2 |
| MS.gene26035.t1 | MTR_8g098915 | 55.714 | 70 | 31 | 0 | 1 | 70 | 3 | 72 | 2.21e-25 | 92.4 |
| MS.gene26035.t1 | MTR_1g099815 | 46.610 | 118 | 46 | 4 | 1 | 103 | 3 | 118 | 1.21e-23 | 88.2 |
| MS.gene26035.t1 | MTR_1g102240 | 46.018 | 113 | 45 | 3 | 1 | 98 | 3 | 114 | 2.69e-22 | 84.7 |
| MS.gene26035.t1 | MTR_1g099640 | 42.982 | 114 | 53 | 2 | 1 | 102 | 3 | 116 | 3.90e-22 | 84.3 |
| MS.gene26035.t1 | MTR_0795s0020 | 54.412 | 68 | 25 | 1 | 1 | 68 | 3 | 64 | 1.66e-19 | 77.4 |
| MS.gene26035.t1 | MTR_7g079110 | 48.485 | 66 | 34 | 0 | 1 | 66 | 3 | 68 | 6.24e-19 | 77.4 |
| MS.gene26035.t1 | MTR_1g099810 | 62.000 | 50 | 19 | 0 | 18 | 67 | 1 | 50 | 3.38e-18 | 73.9 |
| MS.gene26035.t1 | MTR_8g098915 | 58.491 | 53 | 22 | 0 | 18 | 70 | 1 | 53 | 5.28e-18 | 73.2 |
| MS.gene26035.t1 | MTR_8g028615 | 51.064 | 47 | 23 | 0 | 20 | 66 | 22 | 68 | 6.25e-13 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26035.t1 | AT1G01490 | 50.000 | 68 | 33 | 1 | 1 | 67 | 3 | 70 | 7.04e-19 | 77.4 |
| MS.gene26035.t1 | AT1G01490 | 50.000 | 68 | 33 | 1 | 1 | 67 | 3 | 70 | 7.04e-19 | 77.4 |
| MS.gene26035.t1 | AT1G01490 | 50.000 | 68 | 33 | 1 | 1 | 67 | 30 | 97 | 8.21e-19 | 77.8 |
| MS.gene26035.t1 | AT5G52740 | 46.269 | 67 | 34 | 1 | 2 | 68 | 4 | 68 | 4.59e-14 | 63.5 |
| MS.gene26035.t1 | AT5G52760 | 32.710 | 107 | 66 | 3 | 3 | 107 | 7 | 109 | 2.70e-12 | 58.9 |
| MS.gene26035.t1 | AT5G52750 | 40.000 | 65 | 39 | 0 | 1 | 65 | 5 | 69 | 9.09e-11 | 55.5 |
Find 14 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTGCATACTGCCTTTAT+TGG | 0.181377 | 7.4:+53838049 | None:intergenic |
| AAGACAGCATCCAGCCTTTC+AGG | 0.250035 | 7.4:-53838661 | MS.gene26035:intron |
| GAGAAGAGATTAACATTATC+GGG | 0.360117 | 7.4:-53838082 | MS.gene26035:CDS |
| TAGGTTTCAAAGTATTGAAA+TGG | 0.373201 | 7.4:+53837941 | None:intergenic |
| GGGGTCATCTGATAATAAAA+TGG | 0.395316 | 7.4:+53837917 | None:intergenic |
| AGAGAAGAGATTAACATTAT+CGG | 0.398276 | 7.4:-53838083 | MS.gene26035:CDS |
| TCAATATGTACCTGAAAGGC+TGG | 0.458728 | 7.4:+53838651 | None:intergenic |
| ACAGAAAGTGGTGTTGAAAG+TGG | 0.486257 | 7.4:-53838719 | None:intergenic |
| CAAACTGAAGTAGTTACGAT+TGG | 0.578605 | 7.4:-53838013 | MS.gene26035:CDS |
| TACGATTGGACCTCTAAAAG+AGG | 0.583295 | 7.4:-53837999 | MS.gene26035:CDS |
| AAATTCAATATGTACCTGAA+AGG | 0.592630 | 7.4:+53838647 | None:intergenic |
| CATCTGATAATAAAATGGAT+AGG | 0.609597 | 7.4:+53837922 | None:intergenic |
| AGAAGAGATTAACATTATCG+GGG | 0.638995 | 7.4:-53838081 | MS.gene26035:CDS |
| GAAGAGATTAACATTATCGG+GGG | 0.676086 | 7.4:-53838080 | MS.gene26035:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTATTTTTATTTTGC+AGG | - | chr7.4:53838465-53838484 | MS.gene26035:intron | 10.0% |
| !!! | TAACTTAAATTAATTTGATA+AGG | - | chr7.4:53838283-53838302 | MS.gene26035:intron | 10.0% |
| !!! | TTTTTTATTTTTATTTTGCA+GGG | - | chr7.4:53838466-53838485 | MS.gene26035:intron | 10.0% |
| !! | AAAATTAAAATCAATCACAA+TGG | + | chr7.4:53838244-53838263 | None:intergenic | 15.0% |
| !! | ATAGTATGTAAAGTTTATTT+CGG | + | chr7.4:53838092-53838111 | None:intergenic | 15.0% |
| !!! | TTTTTATTTTTATTTTGCAG+GGG | - | chr7.4:53838467-53838486 | MS.gene26035:intron | 15.0% |
| !! | AATGAATCTTTCATGTTATA+TGG | + | chr7.4:53838448-53838467 | None:intergenic | 20.0% |
| !! | ATTTATTGTATTCAAGAGTT+TGG | - | chr7.4:53838110-53838129 | MS.gene26035:CDS | 20.0% |
| !! | TAGTAATCACAAAAATATTG+TGG | + | chr7.4:53838708-53838727 | None:intergenic | 20.0% |
| !! | TAGTATGTAAAGTTTATTTC+GGG | + | chr7.4:53838091-53838110 | None:intergenic | 20.0% |
| ! | AAATTCAATATGTACCTGAA+AGG | + | chr7.4:53837954-53837973 | None:intergenic | 25.0% |
| ! | AATCACAAAAATATTGTGGT+GGG | + | chr7.4:53838704-53838723 | None:intergenic | 25.0% |
| ! | AGAGAAGAGATTAACATTAT+CGG | - | chr7.4:53838515-53838534 | MS.gene26035:intron | 25.0% |
| ! | CATCTGATAATAAAATGGAT+AGG | + | chr7.4:53838679-53838698 | None:intergenic | 25.0% |
| ! | TAATCACAAAAATATTGTGG+TGG | + | chr7.4:53838705-53838724 | None:intergenic | 25.0% |
| !! | TAGATTTTATTCTCAAACGA+CGG | + | chr7.4:53838390-53838409 | None:intergenic | 25.0% |
| !!! | GTGTCTTATTCATTTTTTGA+AGG | + | chr7.4:53838056-53838075 | None:intergenic | 25.0% |
| !!! | TAGGTTTCAAAGTATTGAAA+TGG | + | chr7.4:53838660-53838679 | None:intergenic | 25.0% |
| AGAAGAGATTAACATTATCG+GGG | - | chr7.4:53838517-53838536 | MS.gene26035:intron | 30.0% | |
| AGTACTACTACTATGTAATG+TGG | - | chr7.4:53837983-53838002 | MS.gene26035:CDS | 30.0% | |
| ATCACAAAAATATTGTGGTG+GGG | + | chr7.4:53838703-53838722 | None:intergenic | 30.0% | |
| GAGAAGAGATTAACATTATC+GGG | - | chr7.4:53838516-53838535 | MS.gene26035:intron | 30.0% | |
| AGAAATTCACACATTAAGCG+AGG | - | chr7.4:53838136-53838155 | MS.gene26035:intron | 35.0% | |
| CAAACTGAAGTAGTTACGAT+TGG | - | chr7.4:53838585-53838604 | MS.gene26035:intron | 35.0% | |
| GAAGAGATTAACATTATCGG+GGG | - | chr7.4:53838518-53838537 | MS.gene26035:intron | 35.0% | |
| GGGGTCATCTGATAATAAAA+TGG | + | chr7.4:53838684-53838703 | None:intergenic | 35.0% | |
| ! | TCTTTCTTCTCCTCTTTTAG+AGG | + | chr7.4:53838612-53838631 | None:intergenic | 35.0% |
| AATGGTATCGTAAGAGCAGA+TGG | + | chr7.4:53838226-53838245 | None:intergenic | 40.0% | |
| AGCTTGCATACTGCCTTTAT+TGG | + | chr7.4:53838552-53838571 | None:intergenic | 40.0% | |
| TCAATATGTACCTGAAAGGC+TGG | + | chr7.4:53837950-53837969 | None:intergenic | 40.0% | |
| ! | TACGATTGGACCTCTAAAAG+AGG | - | chr7.4:53838599-53838618 | MS.gene26035:intron | 40.0% |
| CGAGGACAAGAGAGATATAG+CGG | - | chr7.4:53838154-53838173 | MS.gene26035:intron | 45.0% | |
| GAGGACAAGAGAGATATAGC+GGG | - | chr7.4:53838155-53838174 | MS.gene26035:intron | 45.0% | |
| GGGGGATATCGATCCAATAA+AGG | - | chr7.4:53838536-53838555 | MS.gene26035:intron | 45.0% | |
| AAGACAGCATCCAGCCTTTC+AGG | - | chr7.4:53837937-53837956 | MS.gene26035:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 53837883 | 53838737 | 53837883 | ID=MS.gene26035 |
| chr7.4 | mRNA | 53837883 | 53838737 | 53837883 | ID=MS.gene26035.t1;Parent=MS.gene26035 |
| chr7.4 | exon | 53838662 | 53838737 | 53838662 | ID=MS.gene26035.t1.exon1;Parent=MS.gene26035.t1 |
| chr7.4 | CDS | 53838662 | 53838737 | 53838662 | ID=cds.MS.gene26035.t1;Parent=MS.gene26035.t1 |
| chr7.4 | exon | 53837883 | 53838133 | 53837883 | ID=MS.gene26035.t1.exon2;Parent=MS.gene26035.t1 |
| chr7.4 | CDS | 53837883 | 53838133 | 53837883 | ID=cds.MS.gene26035.t1;Parent=MS.gene26035.t1 |
| Gene Sequence |
| Protein sequence |
