AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050251


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050251.t1 MTR_7g451210 90.123 81 8 0 21 101 3 83 1.03e-48 150
MS.gene050251.t1 MTR_7g451200 85.185 81 12 0 21 101 3 83 9.28e-47 145
MS.gene050251.t1 MTR_1173s0010 85.185 81 12 0 21 101 3 83 1.50e-46 145
MS.gene050251.t1 MTR_3g434960 82.716 81 14 0 21 101 3 83 3.17e-45 142
MS.gene050251.t1 MTR_3g434890 82.716 81 14 0 21 101 3 83 4.91e-45 141
MS.gene050251.t1 MTR_3g435000 82.759 87 12 1 18 101 1 87 1.31e-44 140
MS.gene050251.t1 MTR_6g049260 81.013 79 15 0 21 99 3 81 2.53e-40 129
MS.gene050251.t1 MTR_6g051680 63.235 68 25 0 20 87 2 69 1.14e-28 100
MS.gene050251.t1 MTR_6g049320 65.672 67 23 0 21 87 3 69 9.10e-28 98.2
MS.gene050251.t1 MTR_3g434920 78.571 56 12 0 46 101 2 57 1.02e-27 96.7
MS.gene050251.t1 MTR_6g051690 63.235 68 25 0 20 87 2 69 1.31e-27 97.8
MS.gene050251.t1 MTR_6g049320 64.286 70 25 0 18 87 30 99 1.44e-27 98.6
MS.gene050251.t1 MTR_6g049280 62.687 67 25 0 21 87 3 69 1.08e-26 95.5
MS.gene050251.t1 MTR_6g049280 61.429 70 27 0 18 87 30 99 2.42e-26 95.5
MS.gene050251.t1 MTR_1g099800 64.179 67 24 0 21 87 3 69 6.46e-26 94.0
MS.gene050251.t1 MTR_1g099810 62.687 67 25 0 21 87 3 69 1.69e-25 92.8
MS.gene050251.t1 MTR_8g098915 55.714 70 31 0 21 90 3 72 4.43e-25 91.7
MS.gene050251.t1 MTR_1g099640 52.239 67 32 0 20 86 2 68 2.80e-21 82.0
MS.gene050251.t1 MTR_1g099815 56.522 69 29 1 21 89 3 70 5.77e-21 81.3
MS.gene050251.t1 MTR_1g102240 56.522 69 29 1 21 89 3 70 1.53e-20 80.1
MS.gene050251.t1 MTR_0795s0020 53.623 69 26 1 20 88 2 64 2.35e-19 77.0
MS.gene050251.t1 MTR_7g079110 48.485 66 34 0 21 86 3 68 4.86e-19 77.4
MS.gene050251.t1 MTR_1g099810 62.000 50 19 0 38 87 1 50 1.13e-18 75.1
MS.gene050251.t1 MTR_8g098915 58.491 53 22 0 38 90 1 53 4.31e-18 73.2
MS.gene050251.t1 MTR_8g028615 51.064 47 23 0 40 86 22 68 3.67e-13 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050251.t1 AT1G01490 50.000 68 33 1 21 87 3 70 4.92e-19 77.4
MS.gene050251.t1 AT1G01490 50.000 68 33 1 21 87 3 70 4.92e-19 77.4
MS.gene050251.t1 AT1G01490 50.000 68 33 1 21 87 30 97 6.25e-19 77.8
MS.gene050251.t1 AT5G52740 46.269 67 34 1 22 88 4 68 1.23e-13 62.0

Find 14 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTGCATACTGCCTTTAT+TGG 0.181377 7.1:+52817725 None:intergenic
AAGACAGCATCCAGCCTTTC+AGG 0.250035 7.1:-52818309 MS.gene050251:intron
GAGAAGAGATTAACATTATC+GGG 0.360117 7.1:-52817758 MS.gene050251:CDS
TTCACAATGTTCCTTCTCTA+TGG 0.371780 7.1:+52819094 None:intergenic
AGAGAAGAGATTAACATTAT+CGG 0.398276 7.1:-52817759 MS.gene050251:CDS
TCAATATGTACCTGAAAGGC+TGG 0.458728 7.1:+52818299 None:intergenic
ACAGAAAGTGGTGTTGAAAG+TGG 0.486257 7.1:-52818367 MS.gene050251:intron
CAAACTGAAGTAGTTACGAT+TGG 0.578605 7.1:-52817689 MS.gene050251:CDS
TACGATTGGACCTCTAAAAG+AGG 0.583295 7.1:-52817675 MS.gene050251:CDS
AAATTCAATATGTACCTGAA+AGG 0.592630 7.1:+52818295 None:intergenic
TTATTGAGTTTAAGTGTGAG+TGG 0.614038 7.1:+52819071 None:intergenic
GCAAGAACAGACCATAGAGA+AGG 0.620993 7.1:-52819105 MS.gene050251:CDS
AGAAGAGATTAACATTATCG+GGG 0.638995 7.1:-52817757 MS.gene050251:CDS
GAAGAGATTAACATTATCGG+GGG 0.676086 7.1:-52817756 MS.gene050251:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTATTTTTATTTTGC+AGG - chr7.1:52818242-52818261 MS.gene050251:intron 10.0%
!!! TAACTTAAATTAATTTGATA+AGG - chr7.1:52818060-52818079 MS.gene050251:intron 10.0%
!!! TTTTTTATTTTTATTTTGCA+GGG - chr7.1:52818243-52818262 MS.gene050251:intron 10.0%
!! AAAAGCAAATATAAAATTCA+AGG + chr7.1:52818835-52818854 None:intergenic 15.0%
!! AAAATTAAAATCAATCACAA+TGG + chr7.1:52818021-52818040 None:intergenic 15.0%
!! ATAGTATGTAAAGTTTATTT+CGG + chr7.1:52817869-52817888 None:intergenic 15.0%
!! ATATTACAGCAAAAAAAAAA+AGG + chr7.1:52818761-52818780 None:intergenic 15.0%
!! TTCCATTCTTAAATATTAAA+GGG + chr7.1:52817621-52817640 None:intergenic 15.0%
!!! AATTGGTAACTATTTATATA+AGG + chr7.1:52817392-52817411 None:intergenic 15.0%
!!! TTTTTATTTTTATTTTGCAG+GGG - chr7.1:52818244-52818263 MS.gene050251:intron 15.0%
!! AATGAATCTTTCATGTTATA+TGG + chr7.1:52818225-52818244 None:intergenic 20.0%
!! AATGGTAAAATACAGTTAAT+TGG + chr7.1:52817109-52817128 None:intergenic 20.0%
!! ACTATTTATATAAGGTAGAT+AGG + chr7.1:52817384-52817403 None:intergenic 20.0%
!! ATTTATTGTATTCAAGAGTT+TGG - chr7.1:52817887-52817906 MS.gene050251:intron 20.0%
!! CTTCCATTCTTAAATATTAA+AGG + chr7.1:52817622-52817641 None:intergenic 20.0%
!! TAAAATACAGTTAATTGGTT+AGG + chr7.1:52817104-52817123 None:intergenic 20.0%
!! TAGTAATCACAAAAATATTG+TGG + chr7.1:52818485-52818504 None:intergenic 20.0%
!! TAGTATGTAAAGTTTATTTC+GGG + chr7.1:52817868-52817887 None:intergenic 20.0%
! AAATTCAATATGTACCTGAA+AGG + chr7.1:52817759-52817778 None:intergenic 25.0%
! AATCACAAAAATATTGTGGT+GGG + chr7.1:52818481-52818500 None:intergenic 25.0%
! AATGGAAGTAATTAATTGCT+GGG - chr7.1:52817634-52817653 MS.gene050251:intron 25.0%
! ACGGTTATTAAAATGATGAT+AGG - chr7.1:52817295-52817314 MS.gene050251:intron 25.0%
! AGAGAAGAGATTAACATTAT+CGG - chr7.1:52818292-52818311 MS.gene050251:intron 25.0%
! ATTACTACTATGTTTGATGT+GGG - chr7.1:52818498-52818517 MS.gene050251:intron 25.0%
! CATCTGATAATAAAATGGAT+AGG + chr7.1:52818456-52818475 None:intergenic 25.0%
! GTCCCTTTAATATTTAAGAA+TGG - chr7.1:52817616-52817635 MS.gene050251:intron 25.0%
! TAATCACAAAAATATTGTGG+TGG + chr7.1:52818482-52818501 None:intergenic 25.0%
!! TAGATTTTATTCTCAAACGA+CGG + chr7.1:52818167-52818186 None:intergenic 25.0%
!!! TAGGTTTCAAAGTATTGAAA+TGG + chr7.1:52818437-52818456 None:intergenic 25.0%
!!! TTACACTTTTTAACAGAAAG+TGG - chr7.1:52817672-52817691 MS.gene050251:CDS 25.0%
AAGTTCCTTTACAACAAAAG+AGG + chr7.1:52817249-52817268 None:intergenic 30.0%
ACAAACTTCAACAACAATGA+AGG - chr7.1:52817506-52817525 MS.gene050251:intron 30.0%
AGAAGAGATTAACATTATCG+GGG - chr7.1:52818294-52818313 MS.gene050251:intron 30.0%
AGTACTACTACTATGTAATG+TGG - chr7.1:52817788-52817807 MS.gene050251:CDS 30.0%
ATCACAAAAATATTGTGGTG+GGG + chr7.1:52818480-52818499 None:intergenic 30.0%
CACTTAAACTCAATAACGTA+TGG - chr7.1:52816987-52817006 MS.gene050251:intron 30.0%
CTACTTACTAATGACTAACT+TGG - chr7.1:52819043-52819062 MS.gene050251:intron 30.0%
GAAAATGTGCAATAACAGAT+GGG - chr7.1:52818679-52818698 MS.gene050251:intron 30.0%
GAATGGAAGTAATTAATTGC+TGG - chr7.1:52817633-52817652 MS.gene050251:intron 30.0%
GAGAAGAGATTAACATTATC+GGG - chr7.1:52818293-52818312 MS.gene050251:intron 30.0%
GATGAAGTAAGGATGATAAT+TGG + chr7.1:52817409-52817428 None:intergenic 30.0%
GATTACTACTATGTTTGATG+TGG - chr7.1:52818497-52818516 MS.gene050251:intron 30.0%
GATTCAAACTAACATTCACA+CGG - chr7.1:52817276-52817295 MS.gene050251:intron 30.0%
TAGCTGATTAAGAATTGGTA+AGG + chr7.1:52817484-52817503 None:intergenic 30.0%
TGAAAATGTGCAATAACAGA+TGG - chr7.1:52818678-52818697 MS.gene050251:intron 30.0%
TTATTGAGTTTAAGTGTGAG+TGG + chr7.1:52816983-52817002 None:intergenic 30.0%
! CAATTGACTAGTTGACTTTA+CGG - chr7.1:52817345-52817364 MS.gene050251:intron 30.0%
!! GATTTCCTCTTTTGTTGTAA+AGG - chr7.1:52817241-52817260 MS.gene050251:intron 30.0%
!! GTTTTGCTTTTGAGAAGAAA+TGG + chr7.1:52818954-52818973 None:intergenic 30.0%
!!! ATTGTTTACTTTTTGCTTCG+TGG + chr7.1:52817203-52817222 None:intergenic 30.0%
!!! GTTAGAGCTTGTACTTTTTT+AGG + chr7.1:52817137-52817156 None:intergenic 30.0%
AATGGTATCGTAAGAGCAAA+TGG + chr7.1:52818003-52818022 None:intergenic 35.0%
AGAAACGAGCAAAAACAGAT+GGG + chr7.1:52818559-52818578 None:intergenic 35.0%
AGAAATTCACACATTAAGCG+AGG - chr7.1:52817913-52817932 MS.gene050251:intron 35.0%
CAAACTGAAGTAGTTACGAT+TGG - chr7.1:52818362-52818381 MS.gene050251:CDS 35.0%
GAAGAGATTAACATTATCGG+GGG - chr7.1:52818295-52818314 MS.gene050251:intron 35.0%
GAATAGTTCTCACTAAACTC+TGG - chr7.1:52817009-52817028 MS.gene050251:intron 35.0%
GGGGTCATCTGATAATAAAA+TGG + chr7.1:52818461-52818480 None:intergenic 35.0%
TGTGCTAGCTGATTAAGAAT+TGG + chr7.1:52817489-52817508 None:intergenic 35.0%
TTCACAATGTTCCTTCTCTA+TGG + chr7.1:52816960-52816979 None:intergenic 35.0%
! AATGAAGTGGTGATGAAGTA+AGG + chr7.1:52817420-52817439 None:intergenic 35.0%
! CTTTGAGAATTGTTCTTCTC+CGG + chr7.1:52818705-52818724 None:intergenic 35.0%
! TAACTTATGCTCGGCTATTT+TGG + chr7.1:52818583-52818602 None:intergenic 35.0%
! TCTTTCTTCTCCTCTTTTAG+AGG + chr7.1:52818389-52818408 None:intergenic 35.0%
!! GTGCATAATAAGAAAGCATC+CGG - chr7.1:52818520-52818539 MS.gene050251:intron 35.0%
!! TGCTTTTGAGAAGAAATGGT+TGG + chr7.1:52818950-52818969 None:intergenic 35.0%
!! TTTTGAGAAGAAATGGTTGG+TGG + chr7.1:52818947-52818966 None:intergenic 35.0%
!!! AGAGCTTGTACTTTTTTAGG+TGG + chr7.1:52817134-52817153 None:intergenic 35.0%
!!! AGTGTGTTTTAGCAATGAAG+TGG + chr7.1:52817433-52817452 None:intergenic 35.0%
!!! GAGCTTGTACTTTTTTAGGT+GGG + chr7.1:52817133-52817152 None:intergenic 35.0%
!!! GTACTTTTTTAGGTGGGAAA+TGG + chr7.1:52817127-52817146 None:intergenic 35.0%
AAACATACTTGCCTAGCCAT+CGG + chr7.1:52818732-52818751 None:intergenic 40.0%
AGCTTGCATACTGCCTTTAT+TGG + chr7.1:52818329-52818348 None:intergenic 40.0%
AGCTTTCAGGCAAGAGTTTA+GGG + chr7.1:52817036-52817055 None:intergenic 40.0%
CAGAAACGAGCAAAAACAGA+TGG + chr7.1:52818560-52818579 None:intergenic 40.0%
GATGGGAAAAGAGCAATAAC+CGG + chr7.1:52818542-52818561 None:intergenic 40.0%
TCAATATGTACCTGAAAGGC+TGG + chr7.1:52817755-52817774 None:intergenic 40.0%
TGCGTGAGTTAACTTATGCT+CGG + chr7.1:52818592-52818611 None:intergenic 40.0%
TGTGATGTCTTTGCATGCTT+TGG + chr7.1:52818895-52818914 None:intergenic 40.0%
! AAGACTAGTTGAGAGCTTTC+AGG + chr7.1:52817049-52817068 None:intergenic 40.0%
! ACAGAAAGTGGTGTTGAAAG+TGG - chr7.1:52817684-52817703 MS.gene050251:CDS 40.0%
! TACGATTGGACCTCTAAAAG+AGG - chr7.1:52818376-52818395 MS.gene050251:intron 40.0%
AACATACTTGCCTAGCCATC+GGG + chr7.1:52818731-52818750 None:intergenic 45.0%
ATGTGCAATAACAGATGGGC+CGG - chr7.1:52818683-52818702 MS.gene050251:intron 45.0%
CGAGGACAAGAGAGATATAG+CGG - chr7.1:52817931-52817950 MS.gene050251:intron 45.0%
GAGCTTTCAGGCAAGAGTTT+AGG + chr7.1:52817037-52817056 None:intergenic 45.0%
GAGGACAAGAGAGATATAGC+GGG - chr7.1:52817932-52817951 MS.gene050251:intron 45.0%
GCAAGAACAGACCATAGAGA+AGG - chr7.1:52816946-52816965 MS.gene050251:intron 45.0%
GGGGGATATCGATCCAATAA+AGG - chr7.1:52818313-52818332 MS.gene050251:CDS 45.0%
! CAATTCTCAAAGCATCCCGA+TGG - chr7.1:52818713-52818732 MS.gene050251:intron 45.0%
AAGACAGCATCCAGCCTTTC+AGG - chr7.1:52817742-52817761 MS.gene050251:CDS 50.0%
CAGCGTGAGTTAACTCACGT+TGG - chr7.1:52818654-52818673 MS.gene050251:intron 50.0%
TGCTTCGTGGCTGCATCTCA+TGG + chr7.1:52817190-52817209 None:intergenic 55.0%
! CTCAAAGCATCCCGATGGCT+AGG - chr7.1:52818718-52818737 MS.gene050251:intron 55.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 52816944 52819129 52816944 ID=MS.gene050251
chr7.1 mRNA 52816944 52819129 52816944 ID=MS.gene050251.t1;Parent=MS.gene050251
chr7.1 exon 52819070 52819129 52819070 ID=MS.gene050251.t1.exon1;Parent=MS.gene050251.t1
chr7.1 CDS 52819070 52819129 52819070 ID=cds.MS.gene050251.t1;Parent=MS.gene050251.t1
chr7.1 exon 52818310 52818385 52818310 ID=MS.gene050251.t1.exon2;Parent=MS.gene050251.t1
chr7.1 CDS 52818310 52818385 52818310 ID=cds.MS.gene050251.t1;Parent=MS.gene050251.t1
chr7.1 exon 52817646 52817809 52817646 ID=MS.gene050251.t1.exon3;Parent=MS.gene050251.t1
chr7.1 CDS 52817646 52817809 52817646 ID=cds.MS.gene050251.t1;Parent=MS.gene050251.t1
chr7.1 exon 52816944 52816949 52816944 ID=MS.gene050251.t1.exon4;Parent=MS.gene050251.t1
chr7.1 CDS 52816944 52816949 52816944 ID=cds.MS.gene050251.t1;Parent=MS.gene050251.t1
Gene Sequence

>MS.gene050251

ATGCAAGAACAGACCATAGAGAAGGAACATTGTGAACCACTCACACTTAAACTCAATAACGTATGGAATAGTTCTCACTAAACTCTGGACCCTAAACTCTTGCCTGAAAGCTCTCAACTAGTCTTTAGTCTTGTTTTTTATTTTTTTCTATTTTCTACCTAACCAATTAACTGTATTTTACCATTTCCCACCTAAAAAAGTACAAGCTCTAACTTTAAGCGCGTGTTACATTTATGTATGATACCATGAGATGCAGCCACGAAGCAAAAAGTAAACAATTTTTACAATTATTTGACTGATTTCCTCTTTTGTTGTAAAGGAACTTTCGTGATGATTCAAACTAACATTCACACGGTTATTAAAATGATGATAGGCTTTTGAAGAAAAAAACAACATTTTTTCAATTGACTAGTTGACTTTACGGTCTTGCAAGTCTTCCTATCTACCTTATATAAATAGTTACCAATTATCATCCTTACTTCATCACCACTTCATTGCTAAAACACACTTTACTCAAAACTTCAACATCAACTTGCTCCTTACCAATTCTTAATCAGCTAGCACAAACTTCAACAACAATGAAGGTCAGAATTAGATTCGTGAAATCTATTTTTCTTCTTCACTTTTCTCATTTAATTTATTATGTTTTCATGCATGAATAATGTTTTTGTTGTCCCTTTAATATTTAAGAATGGAAGTAATTAATTGCTGGGTTTTAATTCTTTGATTTACACTTTTTAACAGAAAGTGGTGTTGAAAGTGGACTTTTATAATGATAGAATCAAGCAAAAAGTTATGAAGACAGCATCCAGCCTTTCAGGTACATATTGAATTTCATGTCATAAGTACTACTACTATGTAATGTGGCTAAATTACATGACTTATAAGTTGTATGCATGAATAAGACACTTATTTTCATATCCCGAAATAAACTTTACATACTATTTATTGTATTCAAGAGTTTGGTAGAGAAATTCACACATTAAGCGAGGACAAGAGAGATATAGCGGGATCGATCGTGCAACTAAACATATCAAATGTTCTCGTAATCTTTTACCATTTGCTCTTACGATACCATTGTGATTGATTTTAATTTTTGAAGACATAACATGATTCTAACTTAAATTAATTTGATAAGGTCTATTAATATCACAATCTAACTTTATATATAAAAAAAAAATTATAGTTATTTTATAACTATTTCAAATAGTATTTGAACTCCGTCGTTTGAGAATAAAATCTAATTTAATTTCACTTTTGTCTAAATAAAAAGACAAACCATATAACATGAAAGATTCATTTTTTATTTTTATTTTGCAGGGGTTGAATCAGTATCAATAGACTTGAAAGAGAAGAGATTAACATTATCGGGGGATATCGATCCAATAAAGGCAGTATGCAAGCTAAGAAAGTTGTGTCAAACTGAAGTAGTTACGATTGGACCTCTAAAAGAGGAGAAGAAAGAGTCAACAAATACAAATGAAGTTATTCCATTTCAATACTTTGAAACCTATCCATTTTATTATCAGATGACCCCACCACAATATTTTTGTGATTACTACTATGTTTGATGTGGGTGCATAATAAGAAAGCATCCGGTTATTGCTCTTTTCCCATCTGTTTTTGCTCGTTTCTGCCAAAATAGCCGAGCATAAGTTAACTCACGCAAGTGCATTTTCAACGTGAGTTAACTTACGTAGATGTATTTTTCAGCGTGAGTTAACTCACGTTGGATGAAAATGTGCAATAACAGATGGGCCGGAGAAGAACAATTCTCAAAGCATCCCGATGGCTAGGCAAGTATGTTTGATAAACCTTTTTTTTTTTGCTGTAATATCTGCTAAATTAAAGAGCAACATAGAGTTTTATAAAATATGTCTTGTGTTTTCCTTGAATTTTATATTTGCTTTTTTATGATTTAGAATTGTATTTCTTTTTTCTTGCTAAACCAAAGCATGCAAAGACATCACACGAAATTGATCGTCTGAGTTATATTAACACCACCAACCATTTCTTCTCAAAAGCAAAACAAAAATAAATAAGATAAAAGATATGTTGTGTTTGAAACATGTAAATTCAACTAGTGCTTGAACTTGTTACTACTTACTAATGACTAACTTGGTCAATATATCAACTGCATTCTTTGAAGTTTGAATTTTGTTTAAATAATATCTATCTAGATGTAA

Protein sequence

>MS.gene050251.t1

MQEQTIEKEHCEPLTLKLNNKVVLKVDFYNDRIKQKVMKTASSLSGVESVSIDLKEKRLTLSGDIDPIKAVCKLRKLCQTEVVTIGPLKEEKKESTNTNEM