Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05642.t1 | KEH34137.1 | 97.1 | 70 | 2 | 0 | 1 | 70 | 1 | 70 | 1.50E-28 | 134.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05642.t1 | Q9ASY8 | 64.0 | 75 | 22 | 2 | 1 | 70 | 1 | 75 | 9.5e-19 | 93.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05642.t1 | A0A072UWZ8 | 97.1 | 70 | 2 | 0 | 1 | 70 | 1 | 70 | 1.1e-28 | 134.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050303 | MS.gene05642 | 0.804068 | 2.58E-49 | -1.69E-46 |
| MS.gene05642 | MS.gene24692 | 0.806873 | 6.66E-50 | -1.69E-46 |
| MS.gene05642 | MS.gene33129 | 0.802161 | 6.40E-49 | -1.69E-46 |
| MS.gene05642 | MS.gene68119 | 0.806744 | 7.09E-50 | -1.69E-46 |
| MS.gene05642 | MS.gene78054 | 0.812008 | 5.26E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05642.t1 | MTR_3g462060 | 97.143 | 70 | 2 | 0 | 1 | 70 | 1 | 70 | 2.46e-44 | 137 |
| MS.gene05642.t1 | MTR_3g055500 | 94.203 | 69 | 4 | 0 | 1 | 69 | 1 | 69 | 1.24e-42 | 134 |
| MS.gene05642.t1 | MTR_2g067370 | 92.857 | 70 | 5 | 0 | 1 | 70 | 1 | 70 | 1.42e-42 | 132 |
| MS.gene05642.t1 | MTR_3g061970 | 84.286 | 70 | 11 | 0 | 1 | 70 | 1 | 70 | 3.74e-37 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05642.t1 | AT5G41685 | 58.667 | 75 | 26 | 1 | 1 | 70 | 1 | 75 | 3.97e-25 | 88.6 |
| MS.gene05642.t1 | AT1G64220 | 72.727 | 44 | 12 | 0 | 27 | 70 | 34 | 77 | 3.51e-19 | 73.9 |
Find 16 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTGAGAAAGTTGTGGTTT+TGG | 0.300242 | 3.2:-53168686 | None:intergenic |
| ACGAAGGTTATCGCTCACTA+TGG | 0.379788 | 3.2:+53168623 | MS.gene05642:CDS |
| GGCGTCTACGATTTCTCTAA+AGG | 0.498302 | 3.2:+53168514 | MS.gene05642:CDS |
| TACGATTTCTCTAAAGGCAA+AGG | 0.522582 | 3.2:+53168520 | MS.gene05642:CDS |
| CTGTGAAGGAATGGACAACA+TGG | 0.524007 | 3.2:+53168588 | MS.gene05642:CDS |
| ACGATTTCTCTAAAGGCAAA+GGG | 0.528013 | 3.2:+53168521 | MS.gene05642:CDS |
| ATTCCTTTGATTATCATCAT+CGG | 0.538421 | 3.2:+53168650 | MS.gene05642:CDS |
| TCATCGATTCTGTGAAGGAA+TGG | 0.539433 | 3.2:+53168579 | MS.gene05642:CDS |
| CTGAGAAGCTGAGAAAGTTG+TGG | 0.598612 | 3.2:-53168692 | None:intergenic |
| ATCAGTCATCGATTCTGTGA+AGG | 0.601084 | 3.2:+53168574 | MS.gene05642:CDS |
| ATACCGATGATGATAATCAA+AGG | 0.603457 | 3.2:-53168653 | None:intergenic |
| TGTGAAGGAATGGACAACAT+GGG | 0.649737 | 3.2:+53168589 | MS.gene05642:CDS |
| GTGAAGGAATGGACAACATG+GGG | 0.691902 | 3.2:+53168590 | MS.gene05642:CDS |
| ATGGGGTATGAAGAAAACGA+AGG | 0.703137 | 3.2:+53168607 | MS.gene05642:CDS |
| ATGTGCACATCGATTACACA+GGG | 0.703734 | 3.2:-53168715 | None:intergenic |
| AATGTGCACATCGATTACAC+AGG | 0.707397 | 3.2:-53168716 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTCCTTTGATTATCATCAT+CGG | + | chr3.2:53168650-53168669 | MS.gene05642:CDS | 25.0% |
| ATACCGATGATGATAATCAA+AGG | - | chr3.2:53168656-53168675 | None:intergenic | 30.0% | |
| ! | ACGATTTCTCTAAAGGCAAA+GGG | + | chr3.2:53168521-53168540 | MS.gene05642:CDS | 35.0% |
| ! | TACGATTTCTCTAAAGGCAA+AGG | + | chr3.2:53168520-53168539 | MS.gene05642:CDS | 35.0% |
| ATGGGGTATGAAGAAAACGA+AGG | + | chr3.2:53168607-53168626 | MS.gene05642:CDS | 40.0% | |
| TGTGAAGGAATGGACAACAT+GGG | + | chr3.2:53168589-53168608 | MS.gene05642:CDS | 40.0% | |
| ! | AGCTGAGAAAGTTGTGGTTT+TGG | - | chr3.2:53168689-53168708 | None:intergenic | 40.0% |
| !! | ATCAGTCATCGATTCTGTGA+AGG | + | chr3.2:53168574-53168593 | MS.gene05642:CDS | 40.0% |
| !! | TCATCGATTCTGTGAAGGAA+TGG | + | chr3.2:53168579-53168598 | MS.gene05642:CDS | 40.0% |
| ACGAAGGTTATCGCTCACTA+TGG | + | chr3.2:53168623-53168642 | MS.gene05642:CDS | 45.0% | |
| CTGAGAAGCTGAGAAAGTTG+TGG | - | chr3.2:53168695-53168714 | None:intergenic | 45.0% | |
| CTGTGAAGGAATGGACAACA+TGG | + | chr3.2:53168588-53168607 | MS.gene05642:CDS | 45.0% | |
| GGCGTCTACGATTTCTCTAA+AGG | + | chr3.2:53168514-53168533 | MS.gene05642:CDS | 45.0% | |
| GTGAAGGAATGGACAACATG+GGG | + | chr3.2:53168590-53168609 | MS.gene05642:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 53168512 | 53168724 | 53168512 | ID=MS.gene05642 |
| chr3.2 | mRNA | 53168512 | 53168724 | 53168512 | ID=MS.gene05642.t1;Parent=MS.gene05642 |
| chr3.2 | exon | 53168512 | 53168724 | 53168512 | ID=MS.gene05642.t1.exon1;Parent=MS.gene05642.t1 |
| chr3.2 | CDS | 53168512 | 53168724 | 53168512 | ID=cds.MS.gene05642.t1;Parent=MS.gene05642.t1 |
| Gene Sequence |
| Protein sequence |
