Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78054.t1 | XP_003605566.1 | 98.3 | 174 | 3 | 0 | 5 | 178 | 50 | 223 | 1.90E-91 | 345.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78054.t1 | Q9LVA7 | 58.5 | 176 | 67 | 3 | 5 | 177 | 57 | 229 | 2.5e-47 | 189.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78054.t1 | G7JP98 | 98.3 | 174 | 3 | 0 | 5 | 178 | 50 | 223 | 1.4e-91 | 345.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049825 | MS.gene78054 | 0.832469 | 9.28E-56 | -1.69E-46 |
| MS.gene049873 | MS.gene78054 | 0.820796 | 5.68E-53 | -1.69E-46 |
| MS.gene050303 | MS.gene78054 | 0.831446 | 1.66E-55 | -1.69E-46 |
| MS.gene053226 | MS.gene78054 | 0.80842 | 3.13E-50 | -1.69E-46 |
| MS.gene055778 | MS.gene78054 | 0.803742 | 3.02E-49 | -1.69E-46 |
| MS.gene055808 | MS.gene78054 | 0.844438 | 7.56E-59 | -1.69E-46 |
| MS.gene056091 | MS.gene78054 | 0.818105 | 2.33E-52 | -1.69E-46 |
| MS.gene05642 | MS.gene78054 | 0.812008 | 5.26E-51 | -1.69E-46 |
| MS.gene056631 | MS.gene78054 | 0.830854 | 2.32E-55 | -1.69E-46 |
| MS.gene057693 | MS.gene78054 | 0.812194 | 4.79E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene78054 | 0.815526 | 8.84E-52 | -1.69E-46 |
| MS.gene059935 | MS.gene78054 | 0.815163 | 1.06E-51 | -1.69E-46 |
| MS.gene06001 | MS.gene78054 | 0.812499 | 4.11E-51 | -1.69E-46 |
| MS.gene060429 | MS.gene78054 | 0.831235 | 1.87E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78054.t1 | MTR_4g033850 | 96.067 | 178 | 7 | 0 | 1 | 178 | 46 | 223 | 1.18e-124 | 350 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78054.t1 | AT5G62290 | 63.429 | 175 | 59 | 2 | 5 | 177 | 57 | 228 | 7.57e-68 | 206 |
| MS.gene78054.t1 | AT5G62290 | 63.068 | 176 | 59 | 3 | 5 | 177 | 57 | 229 | 3.88e-64 | 197 |
Find 30 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCCAATTATGTTCTTCTTC+TGG | 0.260205 | 4.1:+64928027 | None:intergenic |
| AGCCAGAAGAAGAACATAAT+TGG | 0.306412 | 4.1:-64928029 | MS.gene78054:CDS |
| CACTGACCATTGTTTGGATC+TGG | 0.327299 | 4.1:+64928136 | None:intergenic |
| TGATAAATCAAGAACATGAT+TGG | 0.356529 | 4.1:+64928991 | None:intergenic |
| AAATAACACTGACCATTGTT+TGG | 0.364316 | 4.1:+64928130 | None:intergenic |
| AATACTCTTGGTCATTCAAA+TGG | 0.441340 | 4.1:-64927827 | MS.gene78054:CDS |
| ATGCATTCACTCAATGATGA+TGG | 0.469357 | 4.1:+64925494 | None:intergenic |
| TTACTTGGTTTAGATTGAAA+CGG | 0.480025 | 4.1:-64929056 | MS.gene78054:intron |
| CATAATCCTACCAATACTCT+TGG | 0.504329 | 4.1:-64927839 | MS.gene78054:CDS |
| ACGAATATCATACACTGAGT+TGG | 0.508584 | 4.1:+64928935 | None:intergenic |
| TGCTGTTTCTAGAGATCCTG+AGG | 0.510779 | 4.1:-64932589 | MS.gene78054:CDS |
| AATCAATGATGAACGATTCG+AGG | 0.518438 | 4.1:-64925551 | MS.gene78054:CDS |
| AGTGATGTGGATAAATCTAA+AGG | 0.519256 | 4.1:-64932645 | MS.gene78054:CDS |
| CAAGGAACAGAGTAAGCCTC+AGG | 0.528786 | 4.1:+64932573 | None:intergenic |
| ATACACTGAGTTGGATCAGA+TGG | 0.532090 | 4.1:+64928944 | None:intergenic |
| ATTTGAATGACCAAGAGTAT+TGG | 0.541848 | 4.1:+64927829 | None:intergenic |
| TGCATTCACTCAATGATGAT+GGG | 0.571616 | 4.1:+64925495 | None:intergenic |
| GCAGAAGAGACTGAGCATGA+TGG | 0.572781 | 4.1:-64925526 | MS.gene78054:CDS |
| GATTGAAACGGAGGCAGATG+AGG | 0.573729 | 4.1:-64929044 | MS.gene78054:CDS |
| GAATGACCAAGAGTATTGGT+AGG | 0.585806 | 4.1:+64927833 | None:intergenic |
| AGTGATATGGGTTAGTGATG+TGG | 0.592873 | 4.1:-64932658 | MS.gene78054:CDS |
| GGATTCTGAATCTGAGTCGT+CGG | 0.599733 | 4.1:+64929012 | None:intergenic |
| CAGATGGAAGATGAAGAAGC+AGG | 0.603696 | 4.1:-64927991 | MS.gene78054:intron |
| TGTTCCTTGTCTCTATACTC+AGG | 0.606576 | 4.1:-64932559 | MS.gene78054:intron |
| CTTGGTTTAGATTGAAACGG+AGG | 0.633602 | 4.1:-64929053 | MS.gene78054:intron |
| CTTAATCCAGATCCAAACAA+TGG | 0.635270 | 4.1:-64928142 | MS.gene78054:intron |
| CCTCAAGGATGTTACGAGCA+AGG | 0.636155 | 4.1:+64927795 | None:intergenic |
| TATCAAATGTCAGAGAGATG+AGG | 0.665977 | 4.1:-64928973 | MS.gene78054:CDS |
| CCTTGCTCGTAACATCCTTG+AGG | 0.675433 | 4.1:-64927795 | MS.gene78054:intron |
| AGTACCTGAGTATAGAGACA+AGG | 0.693724 | 4.1:+64932555 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 64925504 | 64932916 | 64925504 | ID=MS.gene78054 |
| chr4.1 | mRNA | 64925504 | 64932916 | 64925504 | ID=MS.gene78054.t1;Parent=MS.gene78054 |
| chr4.1 | exon | 64932906 | 64932916 | 64932906 | ID=MS.gene78054.t1.exon1;Parent=MS.gene78054.t1 |
| chr4.1 | CDS | 64932906 | 64932916 | 64932906 | ID=cds.MS.gene78054.t1;Parent=MS.gene78054.t1 |
| chr4.1 | exon | 64932560 | 64932683 | 64932560 | ID=MS.gene78054.t1.exon2;Parent=MS.gene78054.t1 |
| chr4.1 | CDS | 64932560 | 64932683 | 64932560 | ID=cds.MS.gene78054.t1;Parent=MS.gene78054.t1 |
| chr4.1 | exon | 64928948 | 64929065 | 64928948 | ID=MS.gene78054.t1.exon3;Parent=MS.gene78054.t1 |
| chr4.1 | CDS | 64928948 | 64929065 | 64928948 | ID=cds.MS.gene78054.t1;Parent=MS.gene78054.t1 |
| chr4.1 | exon | 64928143 | 64928202 | 64928143 | ID=MS.gene78054.t1.exon4;Parent=MS.gene78054.t1 |
| chr4.1 | CDS | 64928143 | 64928202 | 64928143 | ID=cds.MS.gene78054.t1;Parent=MS.gene78054.t1 |
| chr4.1 | exon | 64927992 | 64928054 | 64927992 | ID=MS.gene78054.t1.exon5;Parent=MS.gene78054.t1 |
| chr4.1 | CDS | 64927992 | 64928054 | 64927992 | ID=cds.MS.gene78054.t1;Parent=MS.gene78054.t1 |
| chr4.1 | exon | 64927796 | 64927881 | 64927796 | ID=MS.gene78054.t1.exon6;Parent=MS.gene78054.t1 |
| chr4.1 | CDS | 64927796 | 64927881 | 64927796 | ID=cds.MS.gene78054.t1;Parent=MS.gene78054.t1 |
| chr4.1 | exon | 64925504 | 64925578 | 64925504 | ID=MS.gene78054.t1.exon7;Parent=MS.gene78054.t1 |
| chr4.1 | CDS | 64925504 | 64925578 | 64925504 | ID=cds.MS.gene78054.t1;Parent=MS.gene78054.t1 |
| Gene Sequence |
| Protein sequence |
