AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056433

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056433.t1 MTR_8g069575 96.594 411 14 0 1 411 1 411 0.0 836
MS.gene056433.t1 MTR_5g033650 78.022 364 78 2 49 411 4 366 0.0 593
MS.gene056433.t1 MTR_3g077430 69.713 383 113 3 30 411 85 465 0.0 574
MS.gene056433.t1 MTR_8g069610 65.459 414 140 1 1 411 1 414 0.0 570
MS.gene056433.t1 MTR_4g105400 71.920 349 95 2 65 411 11 358 0.0 541
MS.gene056433.t1 MTR_8g069600 63.789 417 121 3 1 411 1 393 0.0 538
MS.gene056433.t1 MTR_3g095890 63.373 415 135 6 1 410 31 433 0.0 524
MS.gene056433.t1 MTR_8g069720 58.753 417 156 6 1 411 1 407 1.92e-178 503
MS.gene056433.t1 MTR_8g069580 59.427 419 137 8 1 411 1 394 1.72e-171 485
MS.gene056433.t1 MTR_7g116420 54.588 425 167 6 12 410 15 439 4.96e-165 471
MS.gene056433.t1 MTR_8g069640 56.039 414 168 4 1 410 1 404 1.51e-163 465
MS.gene056433.t1 MTR_8g069660 55.422 415 166 6 1 411 1 400 9.00e-160 456
MS.gene056433.t1 MTR_8g088510 53.538 424 161 6 8 410 7 415 1.01e-158 454
MS.gene056433.t1 MTR_1g079730 52.204 431 172 6 14 411 22 451 1.05e-158 455
MS.gene056433.t1 MTR_2g062610 53.627 386 158 3 46 411 42 426 7.24e-154 442
MS.gene056433.t1 MTR_8g069590 64.169 307 107 3 106 411 1 305 2.54e-144 413
MS.gene056433.t1 MTR_8g069715 55.108 372 155 4 45 411 46 410 2.71e-141 409
MS.gene056433.t1 MTR_2g062610 57.143 308 121 2 114 411 30 336 1.31e-135 392
MS.gene056433.t1 MTR_2g062610 57.143 308 121 2 114 411 37 343 2.62e-135 391
MS.gene056433.t1 MTR_2g073040 64.444 270 89 3 20 286 4 269 1.71e-122 356
MS.gene056433.t1 MTR_2g073020 61.484 283 98 4 11 286 4 282 3.45e-118 345
MS.gene056433.t1 MTR_2g093840 57.632 321 120 8 95 411 52 360 3.04e-116 343
MS.gene056433.t1 MTR_6g012620 41.624 394 189 10 49 411 27 410 2.37e-97 297
MS.gene056433.t1 MTR_3g104000 38.889 396 214 7 42 411 24 417 1.73e-90 280
MS.gene056433.t1 MTR_3g104030 40.365 384 203 8 45 407 32 410 3.29e-90 279
MS.gene056433.t1 MTR_3g103980 37.644 433 230 11 3 411 19 435 1.96e-89 277
MS.gene056433.t1 MTR_3g104070 38.734 395 207 10 45 411 35 422 1.00e-88 275
MS.gene056433.t1 MTR_8g069695 48.800 250 123 3 164 411 13 259 5.44e-83 255
MS.gene056433.t1 MTR_1g016910 35.267 431 238 8 9 411 3 420 1.84e-76 243
MS.gene056433.t1 MTR_3g104030 39.564 321 161 7 113 407 33 346 3.44e-71 228
MS.gene056433.t1 MTR_5g078580 34.447 389 223 12 7 388 4 367 2.60e-56 190
MS.gene056433.t1 MTR_8g040850 32.432 407 241 12 15 407 4 390 2.65e-55 187
MS.gene056433.t1 MTR_8g069630 63.846 130 45 2 283 411 1 129 1.95e-53 174
MS.gene056433.t1 MTR_8g040890 31.616 427 246 12 14 405 3 418 2.44e-52 181
MS.gene056433.t1 MTR_8g069750 61.607 112 43 0 219 330 151 262 3.91e-45 157
MS.gene056433.t1 MTR_5g094280 35.231 281 158 9 39 314 17 278 8.17e-43 153
MS.gene056433.t1 MTR_5g092260 30.000 400 239 14 14 407 20 384 2.67e-42 153
MS.gene056433.t1 MTR_3g108680 30.168 358 222 13 58 407 49 386 1.73e-41 151
MS.gene056433.t1 MTR_2g046390 30.899 356 209 11 63 405 37 368 4.12e-39 144
MS.gene056433.t1 MTR_8g104480 30.682 352 202 12 73 405 9 337 2.72e-38 141
MS.gene056433.t1 MTR_8g012985 37.083 240 135 9 176 405 4 237 1.80e-37 136
MS.gene056433.t1 MTR_2g046520 34.979 243 143 9 175 407 3 240 1.24e-33 126
MS.gene056433.t1 MTR_2g046430 34.979 243 143 9 175 407 3 240 3.63e-33 125
MS.gene056433.t1 MTR_8g069665 57.407 108 44 2 44 150 86 192 3.30e-32 121
MS.gene056433.t1 MTR_2g046300 29.348 368 184 14 63 405 37 353 1.52e-31 123
MS.gene056433.t1 MTR_2g073050 71.831 71 19 1 341 411 1 70 2.79e-29 108
MS.gene056433.t1 MTR_5g065260 31.897 232 149 5 176 405 2 226 7.07e-28 110
MS.gene056433.t1 MTR_8g069950 80.000 55 11 0 238 292 58 112 1.30e-23 95.5
MS.gene056433.t1 MTR_0009s0020 36.957 138 81 4 220 357 18 149 4.94e-22 92.0
MS.gene056433.t1 MTR_7g072230 32.000 175 107 6 238 403 34 205 5.24e-21 90.9
MS.gene056433.t1 MTR_2g046390 25.141 354 189 12 63 405 37 325 3.37e-19 88.2
MS.gene056433.t1 MTR_5g065920 55.357 56 23 1 165 220 9 62 1.29e-15 71.6
MS.gene056433.t1 MTR_8g069585 52.055 73 31 2 11 80 6 77 1.46e-15 73.2
MS.gene056433.t1 MTR_8g064210 50.649 77 34 2 2 74 15 91 4.23e-14 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056433.t1 AT1G10750 73.037 382 102 1 30 411 87 467 0.0 608
MS.gene056433.t1 AT1G23340 68.238 403 126 2 10 411 8 409 0.0 588
MS.gene056433.t1 AT1G23340 68.238 403 126 2 10 411 8 409 0.0 588
MS.gene056433.t1 AT1G70550 70.712 379 110 1 33 411 88 465 0.0 582
MS.gene056433.t1 AT5G50150 69.372 382 114 3 32 411 40 420 0.0 566
MS.gene056433.t1 AT1G55360 56.477 386 149 5 44 411 38 422 5.63e-160 457
MS.gene056433.t1 AT3G13510 53.753 413 160 7 16 411 21 419 2.34e-158 453
MS.gene056433.t1 AT5G18460 56.510 384 141 5 49 410 50 429 3.23e-157 450
MS.gene056433.t1 AT5G56530 53.461 419 153 7 14 410 21 419 3.46e-153 440
MS.gene056433.t1 AT5G56530 53.461 419 153 7 14 410 21 419 3.46e-153 440
MS.gene056433.t1 AT2G44210 50.957 418 175 7 14 411 8 415 2.09e-147 425
MS.gene056433.t1 AT2G44210 47.768 448 174 8 14 411 8 445 9.18e-142 412
MS.gene056433.t1 AT1G23340 66.906 278 90 2 10 286 8 284 1.42e-136 393
MS.gene056433.t1 AT2G44220 48.058 412 185 9 14 411 7 403 7.21e-134 390
MS.gene056433.t1 AT2G44240 49.737 380 180 7 40 411 26 402 5.67e-125 367
MS.gene056433.t1 AT2G17750 41.849 411 212 10 10 410 2 395 1.30e-112 335
MS.gene056433.t1 AT3G48230 47.733 375 184 8 45 411 3 373 6.17e-112 333
MS.gene056433.t1 AT2G44250 45.477 409 202 10 14 410 9 408 1.10e-108 326
MS.gene056433.t1 AT2G19360 41.763 431 206 10 10 410 10 425 2.05e-107 323
MS.gene056433.t1 AT5G19170 45.506 356 170 7 61 405 23 365 5.97e-103 311
MS.gene056433.t1 AT1G70550 69.268 205 62 1 33 237 88 291 1.20e-102 306
MS.gene056433.t1 AT1G10750 60.526 228 88 2 30 256 87 313 1.05e-93 286
MS.gene056433.t1 AT2G44250 49.186 307 143 7 114 410 22 325 4.24e-89 273
MS.gene056433.t1 AT5G25950 37.385 436 226 9 6 411 14 432 1.23e-82 260
MS.gene056433.t1 AT2G20170 36.983 411 222 15 10 405 8 396 6.07e-74 236
MS.gene056433.t1 AT2G20170 36.983 411 222 15 10 405 8 396 6.07e-74 236
MS.gene056433.t1 AT5G25960 37.500 408 196 8 12 411 1 357 1.36e-73 234
MS.gene056433.t1 AT4G23390 37.772 368 203 13 53 405 41 397 6.47e-67 218
MS.gene056433.t1 AT4G23370 35.279 377 219 12 46 405 33 401 1.35e-66 218
MS.gene056433.t1 AT2G20170 34.793 411 214 15 10 405 8 379 4.48e-65 213
MS.gene056433.t1 AT4G23380 33.090 411 242 13 9 405 7 398 3.75e-62 206
MS.gene056433.t1 AT4G23350 31.989 372 234 9 46 405 36 400 1.85e-59 199
MS.gene056433.t1 AT4G23365 32.527 372 230 11 46 405 36 398 2.78e-59 198
MS.gene056433.t1 AT4G23355 32.533 375 226 11 46 405 36 398 1.91e-57 194
MS.gene056433.t1 AT4G23360 32.891 377 223 12 46 405 36 399 1.73e-56 191
MS.gene056433.t1 AT2G35250 34.311 341 196 9 70 407 24 339 2.55e-52 178
MS.gene056433.t1 AT1G10190 31.643 414 238 20 15 405 3 394 1.46e-46 165
MS.gene056433.t1 AT4G17505 30.704 355 191 13 61 411 18 321 3.44e-45 159
MS.gene056433.t1 AT4G15050 31.034 377 220 17 49 405 38 394 9.52e-43 154
MS.gene056433.t1 AT2G24950 29.730 407 245 17 14 405 29 409 1.61e-40 149
MS.gene056433.t1 AT2G27320 33.542 319 180 10 56 370 15 305 3.54e-39 143
MS.gene056433.t1 AT4G10220 28.029 421 254 15 4 405 8 398 8.09e-39 144
MS.gene056433.t1 AT4G15053 30.233 430 246 20 1 405 1 401 8.64e-39 144
MS.gene056433.t1 AT4G23373 34.800 250 131 9 10 258 7 225 2.56e-34 130
MS.gene056433.t1 AT5G05030 30.028 363 220 13 57 407 19 359 1.81e-33 129
MS.gene056433.t1 AT5G46810 28.000 350 224 11 73 405 11 349 5.02e-33 127
MS.gene056433.t1 AT5G25410 28.056 360 224 13 57 405 28 363 1.32e-32 126
MS.gene056433.t1 AT5G46820 29.191 346 217 12 73 405 17 347 1.65e-32 126
MS.gene056433.t1 AT5G60380 29.155 343 214 13 73 405 44 367 1.14e-30 121
MS.gene056433.t1 AT4G10210 27.119 413 247 16 1 390 5 386 5.19e-30 120
MS.gene056433.t1 AT5G46200 27.532 385 217 15 73 405 31 405 1.19e-29 119
MS.gene056433.t1 AT2G38255 34.762 210 103 6 73 275 14 196 2.28e-28 111
MS.gene056433.t1 AT4G23080 27.804 428 254 20 5 407 9 406 9.47e-28 114
MS.gene056433.t1 AT5G11660 31.058 293 180 11 123 407 26 304 7.07e-27 109
MS.gene056433.t1 AT4G17860 29.745 353 198 18 73 405 29 351 4.45e-26 108
MS.gene056433.t1 AT5G25415 32.787 244 143 8 175 407 99 332 1.15e-24 103
MS.gene056433.t1 AT5G36680 28.383 303 181 12 122 410 51 331 2.31e-23 100
MS.gene056433.t1 AT5G36770 28.383 303 181 12 122 410 51 331 2.31e-23 100
MS.gene056433.t1 AT2G03930 53.125 96 42 3 160 252 38 133 3.44e-23 95.9
MS.gene056433.t1 AT5G04937 29.442 197 112 8 216 405 22 198 4.26e-17 79.7
MS.gene056433.t1 AT5G37520 28.879 232 124 12 181 406 2 198 4.89e-15 73.9

Find 107 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCCTAGATTCTTCACTTAT+TGG 0.171239 8.2:+33521449 MS.gene056433:CDS
ATCCCTATTCACACACTTAA+AGG 0.191236 8.2:+33523280 MS.gene056433:CDS
TCTTGTATATTATAACAATT+TGG 0.235227 8.2:-33523500 None:intergenic
ATGAAGAGTTTCACAAGTTT+AGG 0.287405 8.2:+33519126 MS.gene056433:CDS
TCTCCAACTTCTACATATAA+TGG 0.288121 8.2:+33521698 MS.gene056433:CDS
AATTCAGCTTGTCTCAAATA+TGG 0.288451 8.2:+33520810 MS.gene056433:CDS
CCCATTTGCTTATGCTCTCT+TGG 0.302334 8.2:-33519506 None:intergenic
ATACAAGAAGGAACTAATAA+TGG 0.313088 8.2:+33523515 MS.gene056433:CDS
TTGGTAGAAAAGTTTGATTT+TGG 0.315468 8.2:-33519103 None:intergenic
CAAAATGTCCACTACCCATT+TGG 0.328159 8.2:-33523373 None:intergenic
AGCCACCAATTCCCATGCTT+TGG 0.330284 8.2:-33523218 None:intergenic
GGGAATTGGTGGCTTGAATA+TGG 0.338331 8.2:+33523227 MS.gene056433:CDS
TGGATCATCTCTGGTTCATT+TGG 0.338622 8.2:+33520830 MS.gene056433:CDS
GCTTGCTCTATTAACACATT+TGG 0.344553 8.2:+33519629 MS.gene056433:CDS
AGTGCAACAATGGTTCAATT+TGG 0.351443 8.2:+33523305 MS.gene056433:CDS
TATCCTTTAAGTGTGTGAAT+AGG 0.369475 8.2:-33523283 None:intergenic
TTAGCTACAACATTGAGATT+TGG 0.370876 8.2:-33523473 None:intergenic
TGCTACAATTTACTTTGCTC+TGG 0.378764 8.2:+33521632 MS.gene056433:CDS
GTCCAATAAGTGAAGAATCT+AGG 0.380593 8.2:-33521451 None:intergenic
ATCCTTTAAGTGTGTGAATA+GGG 0.384997 8.2:-33523282 None:intergenic
GTCTCACACTTCTACCCAAA+TGG 0.386568 8.2:+33523358 MS.gene056433:CDS
ACTAGCAGTAATATTGCAAT+TGG 0.395249 8.2:+33521665 MS.gene056433:CDS
TTGATATTAGCTTAATGATC+TGG 0.397300 8.2:+33521729 MS.gene056433:CDS
ACTGATGCATATCAAGGAAC+TGG 0.402596 8.2:+33521608 MS.gene056433:CDS
AACTTGTGAAACTCTTCATT+TGG 0.403131 8.2:-33519122 None:intergenic
ATAGTGAATACAATGTCAAC+AGG 0.406411 8.2:+33523335 MS.gene056433:CDS
CAGGTCAGCCCAGAGCTCTA+TGG 0.408690 8.2:+33521418 MS.gene056433:intron
TATGGATCTGGAAACTTGAT+TGG 0.410366 8.2:+33523245 MS.gene056433:CDS
TCATTAAGCTAATATCAAAT+TGG 0.410645 8.2:-33521724 None:intergenic
CTGGAAACTTGATTGGGTAT+TGG 0.411401 8.2:+33523252 MS.gene056433:CDS
TTTCAATTGTGGAGTTTCTC+AGG 0.412909 8.2:+33519545 MS.gene056433:CDS
AAAATGTCCACTACCCATTT+GGG 0.415939 8.2:-33523372 None:intergenic
CTAATCGGAATCGTTCCTTC+TGG 0.418990 8.2:-33519578 None:intergenic
TGAGACAAGCTGAATTCATA+TGG 0.421304 8.2:-33520803 None:intergenic
ACCAAGAGAGCATAAGCAAA+TGG 0.428963 8.2:+33519505 MS.gene056433:CDS
CATATGGACTTTCCACTTGT+GGG 0.431251 8.2:-33520787 None:intergenic
GCCTGCTGTTAAGACAATTC+AGG 0.432132 8.2:+33519184 MS.gene056433:CDS
GGAGAAGAAAATGAAGGAGG+AGG 0.445382 8.2:-33519037 None:intergenic
TACAAGAAGGAACTAATAAT+GGG 0.448624 8.2:+33523516 MS.gene056433:CDS
TAGCTATCCCCATAGAGCTC+TGG 0.454983 8.2:-33521427 None:intergenic
AAAATTGAAACGTGTTAGAA+TGG 0.456346 8.2:+33519655 MS.gene056433:CDS
TACCATTGAAGCTGGTTGGC+AGG 0.466050 8.2:+33520865 MS.gene056433:CDS
CCTCCATTATATGTAGAAGT+TGG 0.467832 8.2:-33521701 None:intergenic
TGAAGGAGGAGGGAGATGAC+TGG 0.470901 8.2:-33519026 None:intergenic
AAACAAGGAGAAGAAAATGA+AGG 0.472952 8.2:-33519043 None:intergenic
AGCTATCCCCATAGAGCTCT+GGG 0.473291 8.2:-33521426 None:intergenic
TGGTGGCTTGAATATGGATC+TGG 0.475485 8.2:+33523233 MS.gene056433:CDS
AGGATCCAAAGCATGGGAAT+TGG 0.479599 8.2:+33523213 MS.gene056433:intron
TGCCTGCCAACCAGCTTCAA+TGG 0.481927 8.2:-33520867 None:intergenic
AGGTCAGCCCAGAGCTCTAT+GGG 0.482474 8.2:+33521419 MS.gene056433:intron
ACCTGAATTGTCTTAACAGC+AGG 0.484466 8.2:-33519185 None:intergenic
GCAACAATGGTTCAATTTGG+TGG 0.484648 8.2:+33523308 MS.gene056433:CDS
GTGAGTGGGAATGAGTACTA+TGG 0.487457 8.2:+33520737 MS.gene056433:CDS
AAAATTATAACTTTCAATTG+TGG 0.487536 8.2:+33519534 MS.gene056433:CDS
TTTGATCATCCTATGTTGAA+AGG 0.498574 8.2:+33519351 MS.gene056433:CDS
TCTCAAATATGGATCATCTC+TGG 0.501761 8.2:+33520821 MS.gene056433:CDS
CTTTAGAAATATGCAAGTTG+TGG 0.502120 8.2:+33523424 MS.gene056433:CDS
TCCAATAAGTGAAGAATCTA+GGG 0.502742 8.2:-33521450 None:intergenic
GAAGGAGGAGGGAGATGACT+GGG 0.504763 8.2:-33519025 None:intergenic
CCAAGAGAGCATAAGCAAAT+GGG 0.509356 8.2:+33519506 MS.gene056433:CDS
AACATAGGATGATCAAAAGC+TGG 0.509432 8.2:-33519345 None:intergenic
TTATGCTCTCTTGGTCTCTC+CGG 0.511099 8.2:-33519497 None:intergenic
TCATATGGACTTTCCACTTG+TGG 0.512196 8.2:-33520788 None:intergenic
GAGAAGAAAATGAAGGAGGA+GGG 0.526751 8.2:-33519036 None:intergenic
TCTCACACTTCTACCCAAAT+GGG 0.527784 8.2:+33523359 MS.gene056433:CDS
ATCCAAAGCATGGGAATTGG+TGG 0.528426 8.2:+33523216 MS.gene056433:CDS
ATGGATCTGGAAACTTGATT+GGG 0.531742 8.2:+33523246 MS.gene056433:CDS
TTGCAGACTGATGCATATCA+AGG 0.537288 8.2:+33521602 MS.gene056433:intron
TTTATGTGTATAGGATCCTC+CGG 0.539657 8.2:+33519478 MS.gene056433:intron
AAGAGTTTCACAAGTTTAGG+AGG 0.546074 8.2:+33519129 MS.gene056433:CDS
GAAGGAACTAATAATGGGTG+GGG 0.546366 8.2:+33523521 MS.gene056433:CDS
CAAGGAGAAGAAAATGAAGG+AGG 0.547694 8.2:-33519040 None:intergenic
TTGTACCTCATGTCCATCGC+CGG 0.547979 8.2:-33519684 None:intergenic
GATCTCAATACCATTGAAGC+TGG 0.548557 8.2:+33520857 MS.gene056433:CDS
TCAATACCATTGAAGCTGGT+TGG 0.549014 8.2:+33520861 MS.gene056433:CDS
AGAAGGAACTAATAATGGGT+GGG 0.554565 8.2:+33523520 MS.gene056433:CDS
TACAACATTGAGATTTGGCA+AGG 0.560466 8.2:-33523468 None:intergenic
TGCGTGTAGGATCCAAAGCA+TGG 0.565093 8.2:+33523206 MS.gene056433:intron
AAATGTGTGGGTCCCACAAG+TGG 0.566747 8.2:+33520775 MS.gene056433:CDS
GTATATTATAACAATTTGGA+CGG 0.567975 8.2:-33523496 None:intergenic
TAGATTCTTCACTTATTGGA+CGG 0.568545 8.2:+33521453 MS.gene056433:CDS
TTTACTATGGTGGTCCTGGA+AGG 0.570062 8.2:+33523552 MS.gene056433:CDS
ACTTCTACCCAAATGGGTAG+TGG 0.571003 8.2:+33523365 MS.gene056433:CDS
AATGATGCACAGCATGCAGT+TGG 0.572287 8.2:+33520710 MS.gene056433:intron
CTTAAAGGATAGTGCAACAA+TGG 0.576183 8.2:+33523295 MS.gene056433:CDS
TGCTCTCTTGGTCTCTCCGG+AGG 0.583271 8.2:-33519494 None:intergenic
CAAAAGCTAGCATAAATGTG+TGG 0.585357 8.2:+33520762 MS.gene056433:CDS
AGAATGGACACCACCGGCGA+TGG 0.585596 8.2:+33519671 MS.gene056433:CDS
AATTGTTATAATATACAAGA+AGG 0.590507 8.2:+33523503 MS.gene056433:CDS
GTTTATTCTTCAGAAACAAG+TGG 0.596638 8.2:+33519080 MS.gene056433:CDS
CACCACCGGCGATGGACATG+AGG 0.599402 8.2:+33519679 MS.gene056433:CDS
CTGATGCATATCAAGGAACT+GGG 0.602205 8.2:+33521609 MS.gene056433:CDS
AAGAAGGAACTAATAATGGG+TGG 0.603081 8.2:+33523519 MS.gene056433:CDS
AAGTGTGTGAATAGGGATGA+TGG 0.604788 8.2:-33523275 None:intergenic
TACCTCATGTCCATCGCCGG+TGG 0.612456 8.2:-33519681 None:intergenic
AAAAGCTAGCATAAATGTGT+GGG 0.619969 8.2:+33520763 MS.gene056433:CDS
TATTAGCTTAATGATCTGGA+AGG 0.620476 8.2:+33521733 MS.gene056433:CDS
CATGACAAACCAAAGAAACA+AGG 0.620517 8.2:-33519058 None:intergenic
TCAGGTGAATCATGTCCAGA+AGG 0.626410 8.2:+33519563 MS.gene056433:CDS
GCGTGTAGGATCCAAAGCAT+GGG 0.628244 8.2:+33523207 MS.gene056433:intron
ACAACATTGAGATTTGGCAA+GGG 0.654257 8.2:-33523467 None:intergenic
CGTGTTAGAATGGACACCAC+CGG 0.654611 8.2:+33519665 MS.gene056433:CDS
CCAACTTCTACATATAATGG+AGG 0.662547 8.2:+33521701 MS.gene056433:CDS
ATGCAGTTGGATATGTGAGT+GGG 0.674700 8.2:+33520723 MS.gene056433:CDS
CATGCAGTTGGATATGTGAG+TGG 0.677188 8.2:+33520722 MS.gene056433:CDS
TAGTGAATACAATGTCAACA+GGG 0.716345 8.2:+33523336 MS.gene056433:CDS
GGTCAGCCCAGAGCTCTATG+GGG 0.745363 8.2:+33521420 MS.gene056433:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATATGATAAATTATG+TGG - chr8.2:33522673-33522692 None:intergenic 10.0%
!! TGTATTTAAAAAAATGATAA+TGG + chr8.2:33521569-33521588 MS.gene056433:intron 10.0%
!!! TCTTTTGAAATAATTAATAT+CGG + chr8.2:33520231-33520250 MS.gene056433:intron 10.0%
!!! TCTTTTTTAAACTATTAAAA+AGG + chr8.2:33523082-33523101 MS.gene056433:intron 10.0%
!!! TTTTATCAGAATATTATTTT+TGG - chr8.2:33520441-33520460 None:intergenic 10.0%
!! AAAAAGAAAAAGAAAACATT+TGG - chr8.2:33520273-33520292 None:intergenic 15.0%
!! AAAAATGAAGGAATAAAAAA+AGG - chr8.2:33522786-33522805 None:intergenic 15.0%
!! AAAATTATAACTTTCAATTG+TGG + chr8.2:33519534-33519553 MS.gene056433:CDS 15.0%
!! AAATTGATTATAAATGTGAT+CGG - chr8.2:33521852-33521871 None:intergenic 15.0%
!! AACAAATAGAACAATTAATT+TGG + chr8.2:33521204-33521223 MS.gene056433:intron 15.0%
!! AATTGTTATAATATACAAGA+AGG + chr8.2:33523503-33523522 MS.gene056433:CDS 15.0%
!! ATTATTTCAAAAGAAATGAA+TGG - chr8.2:33520225-33520244 None:intergenic 15.0%
!! ATTTATATTAGATTCAATGT+TGG - chr8.2:33520641-33520660 None:intergenic 15.0%
!! CTATTGAAGATAAAAATAAT+TGG - chr8.2:33520594-33520613 None:intergenic 15.0%
!! TATTTCAACTTATAATTGTT+CGG + chr8.2:33519454-33519473 MS.gene056433:intron 15.0%
!! TCTTGTATATTATAACAATT+TGG - chr8.2:33523503-33523522 None:intergenic 15.0%
!! TGAAAAAAAGAAACAAAAAA+GGG - chr8.2:33522650-33522669 None:intergenic 15.0%
!! TTAAAAACTGTTTATAAATG+TGG + chr8.2:33521954-33521973 MS.gene056433:intron 15.0%
!! TTTATACATATCAAGAAAAA+TGG - chr8.2:33521181-33521200 None:intergenic 15.0%
!!! AAAGTATCATTATTAAGTAT+TGG - chr8.2:33521126-33521145 None:intergenic 15.0%
!!! AAATGATCAAATTTCTTTTA+CGG - chr8.2:33520011-33520030 None:intergenic 15.0%
!!! AATATTTTGTAACAAATCAA+TGG - chr8.2:33522230-33522249 None:intergenic 15.0%
!!! ATTTTTTACTTGTCATTTTA+AGG + chr8.2:33520982-33521001 MS.gene056433:intron 15.0%
!!! CAATGTTTTTAATTTTGTAT+TGG + chr8.2:33521364-33521383 MS.gene056433:intron 15.0%
!!! CATCTATTTTTTCTTAAAAA+AGG - chr8.2:33519242-33519261 None:intergenic 15.0%
!!! CTTTTTTAAGAAAAAATAGA+TGG + chr8.2:33519240-33519259 MS.gene056433:intron 15.0%
!!! TTTTATGTATCTTTTCATTA+TGG + chr8.2:33519772-33519791 MS.gene056433:intron 15.0%
!!! TTTTCACATTCATTAAATAA+CGG - chr8.2:33521933-33521952 None:intergenic 15.0%
!!! TTTTGGCTTATATAAAATTA+CGG + chr8.2:33519941-33519960 MS.gene056433:intron 15.0%
!! AAAACATTGATGAAAATGAA+AGG - chr8.2:33521353-33521372 None:intergenic 20.0%
!! AAAACTAAGAAAAATACCAA+TGG - chr8.2:33519384-33519403 None:intergenic 20.0%
!! AACTTAACATAGATAGATAA+TGG + chr8.2:33519840-33519859 MS.gene056433:intron 20.0%
!! ACGAAAATTCTTAAATTGAT+TGG + chr8.2:33521283-33521302 MS.gene056433:intron 20.0%
!! AGTAAAATACGCAAAATATT+AGG - chr8.2:33522826-33522845 None:intergenic 20.0%
!! ATCTAGATTATAATATAGTC+TGG + chr8.2:33521901-33521920 MS.gene056433:intron 20.0%
!! ATTCTTAAATTGATTGGATT+TGG + chr8.2:33521289-33521308 MS.gene056433:intron 20.0%
!! GTAAAATACGCAAAATATTA+GGG - chr8.2:33522825-33522844 None:intergenic 20.0%
!! GTATATTATAACAATTTGGA+CGG - chr8.2:33523499-33523518 None:intergenic 20.0%
!! GTGAAAAAAAGAAACAAAAA+AGG - chr8.2:33522651-33522670 None:intergenic 20.0%
!! GTTAAATAAGAGAAAAATGA+AGG - chr8.2:33522798-33522817 None:intergenic 20.0%
!! TATCAAGAAAAATGGATAAA+TGG - chr8.2:33521173-33521192 None:intergenic 20.0%
!! TCATTAAGCTAATATCAAAT+TGG - chr8.2:33521727-33521746 None:intergenic 20.0%
!!! AAATCAATGGTTAGTTAATT+TGG - chr8.2:33522217-33522236 None:intergenic 20.0%
!!! AACAATTAATTTGGAAAGAA+GGG + chr8.2:33521213-33521232 MS.gene056433:intron 20.0%
!!! AATAATCTCATTGACTAAAT+TGG - chr8.2:33522124-33522143 None:intergenic 20.0%
!!! AGTTTAAGTAATTTTTATGC+AGG + chr8.2:33521399-33521418 MS.gene056433:intron 20.0%
!!! ATAAAGTACTAACAACAAAT+TGG + chr8.2:33520679-33520698 MS.gene056433:intron 20.0%
!!! ATCTATGTTAAGTTGTTTTT+TGG - chr8.2:33519834-33519853 None:intergenic 20.0%
!!! TCATTATTAAGTATTGGATT+TGG - chr8.2:33521120-33521139 None:intergenic 20.0%
!!! TCTATGTTAAGTTGTTTTTT+GGG - chr8.2:33519833-33519852 None:intergenic 20.0%
!!! TCTTCTTTTATTCTTCTAAT+CGG - chr8.2:33519596-33519615 None:intergenic 20.0%
! AAAATGACCTTAGAAGATTA+TGG + chr8.2:33521777-33521796 MS.gene056433:intron 25.0%
! AAAATTGAAACGTGTTAGAA+TGG + chr8.2:33519655-33519674 MS.gene056433:CDS 25.0%
! AAATGACCTTAGAAGATTAT+GGG + chr8.2:33521778-33521797 MS.gene056433:intron 25.0%
! AAATTACCCATAATCTTCTA+AGG - chr8.2:33521787-33521806 None:intergenic 25.0%
! AAGTAAATTGATTGAAGCAA+GGG + chr8.2:33521477-33521496 MS.gene056433:intron 25.0%
! AATGACAAGTAAAAAATGAG+TGG - chr8.2:33520979-33520998 None:intergenic 25.0%
! AGTAGTAAAGTGAGTAAATA+TGG - chr8.2:33522531-33522550 None:intergenic 25.0%
! AGTCAAAAAGAAGAAAACAA+AGG - chr8.2:33522862-33522881 None:intergenic 25.0%
! ATACAAGAAGGAACTAATAA+TGG + chr8.2:33523515-33523534 MS.gene056433:CDS 25.0%
! ATGACAAGTAAAAAATGAGT+GGG - chr8.2:33520978-33520997 None:intergenic 25.0%
! ATTGAAAATAAGTACTCCAT+CGG - chr8.2:33521981-33522000 None:intergenic 25.0%
! ATTTACTCACTTTACTACTT+TGG + chr8.2:33522532-33522551 MS.gene056433:intron 25.0%
! GAAAATTTAAGCTGAATATG+TGG + chr8.2:33522276-33522295 MS.gene056433:intron 25.0%
! GTTCAAATGTTAGTGAATTT+AGG + chr8.2:33522503-33522522 MS.gene056433:intron 25.0%
! TAAGTAAATTGATTGAAGCA+AGG + chr8.2:33521476-33521495 MS.gene056433:intron 25.0%
! TAATGAGACTTTCAAACTAA+TGG - chr8.2:33522330-33522349 None:intergenic 25.0%
! TACAAGAAGGAACTAATAAT+GGG + chr8.2:33523516-33523535 MS.gene056433:CDS 25.0%
! TCACTTCTCAGTTTATTAAT+AGG + chr8.2:33520564-33520583 MS.gene056433:intron 25.0%
! TGACCACTTTAAATTGAAAA+AGG - chr8.2:33520527-33520546 None:intergenic 25.0%
! TTAGAAGATTATGGGTAATT+TGG + chr8.2:33521786-33521805 MS.gene056433:intron 25.0%
!! ATTTTACGTTAAACGTGTTT+TGG + chr8.2:33519924-33519943 MS.gene056433:intron 25.0%
!! CTTTTCATTATGGTAAAGTA+TGG + chr8.2:33519782-33519801 MS.gene056433:intron 25.0%
!! GAAAGAGATAAAAAGCATAA+GGG - chr8.2:33522597-33522616 None:intergenic 25.0%
!! GAACAATTAATTTGGAAAGA+AGG + chr8.2:33521212-33521231 MS.gene056433:intron 25.0%
!! TGAAAGAGATAAAAAGCATA+AGG - chr8.2:33522598-33522617 None:intergenic 25.0%
!! TTGATATTAGCTTAATGATC+TGG + chr8.2:33521729-33521748 MS.gene056433:CDS 25.0%
!!! AGTCCTTTTTCAATTTAAAG+TGG + chr8.2:33520521-33520540 MS.gene056433:intron 25.0%
!!! AGTTTTTTTTCTCGATCAAA+AGG + chr8.2:33519398-33519417 MS.gene056433:intron 25.0%
!!! ATAATCTTCTAAGGTCATTT+TGG - chr8.2:33521778-33521797 None:intergenic 25.0%
!!! CATAGATAGATAATGGAAAT+TGG + chr8.2:33519847-33519866 MS.gene056433:intron 25.0%
!!! CATATGGTAGTATTTTTTGA+GGG + chr8.2:33519875-33519894 MS.gene056433:intron 25.0%
!!! GATTTGGTTTTGCATTAAAT+AGG + chr8.2:33521305-33521324 MS.gene056433:intron 25.0%
!!! TTGGTAGAAAAGTTTGATTT+TGG - chr8.2:33519106-33519125 None:intergenic 25.0%
AAAAGCTAGCATAAATGTGT+GGG + chr8.2:33520763-33520782 MS.gene056433:CDS 30.0%
AAACAAGGAGAAGAAAATGA+AGG - chr8.2:33519046-33519065 None:intergenic 30.0%
AACTTGTGAAACTCTTCATT+TGG - chr8.2:33519125-33519144 None:intergenic 30.0%
AATTCAGCTTGTCTCAAATA+TGG + chr8.2:33520810-33520829 MS.gene056433:CDS 30.0%
ACATACTTTAGTCCTTATGT+AGG - chr8.2:33520073-33520092 None:intergenic 30.0%
ACTAGCAGTAATATTGCAAT+TGG + chr8.2:33521665-33521684 MS.gene056433:CDS 30.0%
AGTAAATACCAGTAGAAACT+TGG - chr8.2:33521823-33521842 None:intergenic 30.0%
ATAGTGAATACAATGTCAAC+AGG + chr8.2:33523335-33523354 MS.gene056433:CDS 30.0%
ATCCTTTAAGTGTGTGAATA+GGG - chr8.2:33523285-33523304 None:intergenic 30.0%
ATGAAGAGTTTCACAAGTTT+AGG + chr8.2:33519126-33519145 MS.gene056433:CDS 30.0%
ATGGAAATTGGTAAAGCATA+TGG + chr8.2:33519859-33519878 MS.gene056433:intron 30.0%
CAACATAAATGACACACAAA+AGG + chr8.2:33522447-33522466 MS.gene056433:intron 30.0%
CTTCTCAGTTTATTAATAGG+TGG + chr8.2:33520567-33520586 MS.gene056433:intron 30.0%
CTTTAAGTCGTAAAAAGCAA+AGG - chr8.2:33522738-33522757 None:intergenic 30.0%
GTATCGTTGAAGATTTACTA+GGG + chr8.2:33522969-33522988 MS.gene056433:intron 30.0%
GTTTATTCTTCAGAAACAAG+TGG + chr8.2:33519080-33519099 MS.gene056433:CDS 30.0%
TAGAATTGTAGAAGAAGCAA+GGG + chr8.2:33522375-33522394 MS.gene056433:intron 30.0%
TAGATTCTTCACTTATTGGA+CGG + chr8.2:33521453-33521472 MS.gene056433:CDS 30.0%
TAGTGAATACAATGTCAACA+GGG + chr8.2:33523336-33523355 MS.gene056433:CDS 30.0%
TATCCTTTAAGTGTGTGAAT+AGG - chr8.2:33523286-33523305 None:intergenic 30.0%
TCCAATAAGTGAAGAATCTA+GGG - chr8.2:33521453-33521472 None:intergenic 30.0%
TCTCCAACTTCTACATATAA+TGG + chr8.2:33521698-33521717 MS.gene056433:CDS 30.0%
TGAAGGAATAAAAAAAGGTG+TGG - chr8.2:33522781-33522800 None:intergenic 30.0%
TGATGAAAATGAAAGGTAGT+TGG - chr8.2:33521346-33521365 None:intergenic 30.0%
TGGCTTATATAAAATTACGG+TGG + chr8.2:33519944-33519963 MS.gene056433:intron 30.0%
TGTATCGTTGAAGATTTACT+AGG + chr8.2:33522968-33522987 MS.gene056433:intron 30.0%
TGTATTAATGTGAGACTACA+AGG - chr8.2:33520391-33520410 None:intergenic 30.0%
TTAGCTACAACATTGAGATT+TGG - chr8.2:33523476-33523495 None:intergenic 30.0%
TTGAAAATAAGTACTCCATC+GGG - chr8.2:33521980-33521999 None:intergenic 30.0%
TTGATTATAAATGTGATCGG+AGG - chr8.2:33521849-33521868 None:intergenic 30.0%
TTTGATCATCCTATGTTGAA+AGG + chr8.2:33519351-33519370 MS.gene056433:CDS 30.0%
! AGACTAACTTTTCTTTTGGA+AGG - chr8.2:33519988-33520007 None:intergenic 30.0%
! CTTTAGAAATATGCAAGTTG+TGG + chr8.2:33523424-33523443 MS.gene056433:CDS 30.0%
! TATTAGCTTAATGATCTGGA+AGG + chr8.2:33521733-33521752 MS.gene056433:CDS 30.0%
!! GGAAATTACTTTTACTATGG+TGG + chr8.2:33523542-33523561 MS.gene056433:CDS 30.0%
!! TGGGGAAATTACTTTTACTA+TGG + chr8.2:33523539-33523558 MS.gene056433:CDS 30.0%
!! TTGTTCGGTTTTATGTGTAT+AGG + chr8.2:33519469-33519488 MS.gene056433:intron 30.0%
!! TTTTCTTCTCCTTGTTTCTT+TGG + chr8.2:33519049-33519068 MS.gene056433:CDS 30.0%
!!! GCATATGGTAGTATTTTTTG+AGG + chr8.2:33519874-33519893 MS.gene056433:intron 30.0%
!!! TTTGCACTGCTTTTTTTAGT+AGG + chr8.2:33520948-33520967 MS.gene056433:intron 30.0%
!!! TTTTTTGAGGGTAAAGCATA+TGG + chr8.2:33519887-33519906 MS.gene056433:intron 30.0%
AAAATGTCCACTACCCATTT+GGG - chr8.2:33523375-33523394 None:intergenic 35.0%
AAAGTGGTCACTGAATTTCA+TGG + chr8.2:33520537-33520556 MS.gene056433:intron 35.0%
AAATACCAGTAGAAACTTGG+AGG - chr8.2:33521820-33521839 None:intergenic 35.0%
AACATAGGATGATCAAAAGC+TGG - chr8.2:33519348-33519367 None:intergenic 35.0%
AAGAGTTTCACAAGTTTAGG+AGG + chr8.2:33519129-33519148 MS.gene056433:CDS 35.0%
AATACCAGTAGAAACTTGGA+GGG - chr8.2:33521819-33521838 None:intergenic 35.0%
ACAACATTGAGATTTGGCAA+GGG - chr8.2:33523470-33523489 None:intergenic 35.0%
ACCCTAGATTCTTCACTTAT+TGG + chr8.2:33521449-33521468 MS.gene056433:CDS 35.0%
AGTGCAACAATGGTTCAATT+TGG + chr8.2:33523305-33523324 MS.gene056433:CDS 35.0%
ATCCCTATTCACACACTTAA+AGG + chr8.2:33523280-33523299 MS.gene056433:CDS 35.0%
ATGGATCTGGAAACTTGATT+GGG + chr8.2:33523246-33523265 MS.gene056433:CDS 35.0%
ATTAATGTGAGACTACAAGG+AGG - chr8.2:33520388-33520407 None:intergenic 35.0%
CAAAAGCTAGCATAAATGTG+TGG + chr8.2:33520762-33520781 MS.gene056433:CDS 35.0%
CACAATGTTAGTGTTGTGTA+TGG + chr8.2:33523112-33523131 MS.gene056433:intron 35.0%
CATGACAAACCAAAGAAACA+AGG - chr8.2:33519061-33519080 None:intergenic 35.0%
CCAACTTCTACATATAATGG+AGG + chr8.2:33521701-33521720 MS.gene056433:CDS 35.0%
CCATAAAAATGCATCTTCGA+CGG + chr8.2:33519153-33519172 MS.gene056433:CDS 35.0%
CCTCCATTATATGTAGAAGT+TGG - chr8.2:33521704-33521723 None:intergenic 35.0%
CTTAAAGGATAGTGCAACAA+TGG + chr8.2:33523295-33523314 MS.gene056433:CDS 35.0%
GCTTGCTCTATTAACACATT+TGG + chr8.2:33519629-33519648 MS.gene056433:CDS 35.0%
GTAGAATTGTAGAAGAAGCA+AGG + chr8.2:33522374-33522393 MS.gene056433:intron 35.0%
GTCCAATAAGTGAAGAATCT+AGG - chr8.2:33521454-33521473 None:intergenic 35.0%
GTTGAAAGGACAAAAACCAT+TGG + chr8.2:33519365-33519384 MS.gene056433:CDS 35.0%
TAAATTGATTGAAGCAAGGG+AGG + chr8.2:33521480-33521499 MS.gene056433:intron 35.0%
TACAACATTGAGATTTGGCA+AGG - chr8.2:33523471-33523490 None:intergenic 35.0%
TATGGATCTGGAAACTTGAT+TGG + chr8.2:33523245-33523264 MS.gene056433:CDS 35.0%
TCTCAAATATGGATCATCTC+TGG + chr8.2:33520821-33520840 MS.gene056433:CDS 35.0%
TCTCATAGTCAAAGTCTCAA+AGG + chr8.2:33522254-33522273 MS.gene056433:intron 35.0%
TGAGACAAGCTGAATTCATA+TGG - chr8.2:33520806-33520825 None:intergenic 35.0%
TGCTACAATTTACTTTGCTC+TGG + chr8.2:33521632-33521651 MS.gene056433:CDS 35.0%
TTGCTTCTATTTATGCGTGT+AGG + chr8.2:33523193-33523212 MS.gene056433:intron 35.0%
TTTATGTGTATAGGATCCTC+CGG + chr8.2:33519478-33519497 MS.gene056433:intron 35.0%
TTTCAATTGTGGAGTTTCTC+AGG + chr8.2:33519545-33519564 MS.gene056433:CDS 35.0%
! AAGAAGGAACTAATAATGGG+TGG + chr8.2:33523519-33523538 MS.gene056433:CDS 35.0%
! ACGGAGACTAACTTTTCTTT+TGG - chr8.2:33519992-33520011 None:intergenic 35.0%
! GGTTTTTGTCCTTTCAACAT+AGG - chr8.2:33519363-33519382 None:intergenic 35.0%
!! AGAAGAAAACAAAGGTACCA+AGG - chr8.2:33522854-33522873 None:intergenic 35.0%
!! AGAAGGAACTAATAATGGGT+GGG + chr8.2:33523520-33523539 MS.gene056433:CDS 35.0%
!!! GTGATTTTAGCTCCTACATA+AGG + chr8.2:33520058-33520077 MS.gene056433:intron 35.0%
!!! GTTTCTGTTGTTTTCTGTGA+TGG - chr8.2:33519712-33519731 None:intergenic 35.0%
AAGTGTGTGAATAGGGATGA+TGG - chr8.2:33523278-33523297 None:intergenic 40.0%
AATATGTGGTCAATGCATGG+TGG + chr8.2:33522290-33522309 MS.gene056433:intron 40.0%
ACATATGCTTGTGTTCGATG+TGG + chr8.2:33523032-33523051 MS.gene056433:intron 40.0%
ACCTGAATTGTCTTAACAGC+AGG - chr8.2:33519188-33519207 None:intergenic 40.0%
ACTGATGCATATCAAGGAAC+TGG + chr8.2:33521608-33521627 MS.gene056433:CDS 40.0%
ATATCACCATCAAGACTCTG+CGG - chr8.2:33519297-33519316 None:intergenic 40.0%
ATATCCCTCCAAGTTTCTAC+TGG + chr8.2:33521812-33521831 MS.gene056433:intron 40.0%
ATATGTGGTCAATGCATGGT+GGG + chr8.2:33522291-33522310 MS.gene056433:intron 40.0%
ATGCAGTTGGATATGTGAGT+GGG + chr8.2:33520723-33520742 MS.gene056433:CDS 40.0%
CAAAATGTCCACTACCCATT+TGG - chr8.2:33523376-33523395 None:intergenic 40.0%
CAAGGAGAAGAAAATGAAGG+AGG - chr8.2:33519043-33519062 None:intergenic 40.0%
CATATGGACTTTCCACTTGT+GGG - chr8.2:33520790-33520809 None:intergenic 40.0%
CTGAATATGTGGTCAATGCA+TGG + chr8.2:33522287-33522306 MS.gene056433:intron 40.0%
CTGATGCATATCAAGGAACT+GGG + chr8.2:33521609-33521628 MS.gene056433:CDS 40.0%
CTGGAAACTTGATTGGGTAT+TGG + chr8.2:33523252-33523271 MS.gene056433:CDS 40.0%
GAGAAGAAAATGAAGGAGGA+GGG - chr8.2:33519039-33519058 None:intergenic 40.0%
GATCTCAATACCATTGAAGC+TGG + chr8.2:33520857-33520876 MS.gene056433:CDS 40.0%
GCAACAATGGTTCAATTTGG+TGG + chr8.2:33523308-33523327 MS.gene056433:CDS 40.0%
TCAATACCATTGAAGCTGGT+TGG + chr8.2:33520861-33520880 MS.gene056433:CDS 40.0%
TCATATGGACTTTCCACTTG+TGG - chr8.2:33520791-33520810 None:intergenic 40.0%
TCTCACACTTCTACCCAAAT+GGG + chr8.2:33523359-33523378 MS.gene056433:CDS 40.0%
TGTTTATAAATGTGGCCCGA+TGG + chr8.2:33521962-33521981 MS.gene056433:intron 40.0%
TTGCAGACTGATGCATATCA+AGG + chr8.2:33521602-33521621 MS.gene056433:intron 40.0%
! ACCAAGAGAGCATAAGCAAA+TGG + chr8.2:33519505-33519524 MS.gene056433:CDS 40.0%
! CCAAGAGAGCATAAGCAAAT+GGG + chr8.2:33519506-33519525 MS.gene056433:CDS 40.0%
! CCGTCGAAGATGCATTTTTA+TGG - chr8.2:33519156-33519175 None:intergenic 40.0%
! CTCATTGACTAAATTGGCAC+TGG - chr8.2:33522118-33522137 None:intergenic 40.0%
! GGACATTTTACATTCCTTCC+AGG - chr8.2:33523569-33523588 None:intergenic 40.0%
! TACTTTTACTATGGTGGTCC+TGG + chr8.2:33523548-33523567 MS.gene056433:CDS 40.0%
! TGGATCATCTCTGGTTCATT+TGG + chr8.2:33520830-33520849 MS.gene056433:CDS 40.0%
!! AGAAAACAAAGGTACCAAGG+TGG - chr8.2:33522851-33522870 None:intergenic 40.0%
!! GAAGGAACTAATAATGGGTG+GGG + chr8.2:33523521-33523540 MS.gene056433:CDS 40.0%
AATGATGCACAGCATGCAGT+TGG + chr8.2:33520710-33520729 MS.gene056433:intron 45.0%
ACTTCTACCCAAATGGGTAG+TGG + chr8.2:33523365-33523384 MS.gene056433:CDS 45.0%
AGGATCCAAAGCATGGGAAT+TGG + chr8.2:33523213-33523232 MS.gene056433:intron 45.0%
AGGTGTGGCGTTCAGAATTA+TGG - chr8.2:33522766-33522785 None:intergenic 45.0%
ATCCAAAGCATGGGAATTGG+TGG + chr8.2:33523216-33523235 MS.gene056433:CDS 45.0%
ATTGATTGAAGCAAGGGAGG+TGG + chr8.2:33521483-33521502 MS.gene056433:intron 45.0%
CATGCAGTTGGATATGTGAG+TGG + chr8.2:33520722-33520741 MS.gene056433:CDS 45.0%
CCCATTTGCTTATGCTCTCT+TGG - chr8.2:33519509-33519528 None:intergenic 45.0%
CTAATCGGAATCGTTCCTTC+TGG - chr8.2:33519581-33519600 None:intergenic 45.0%
GCCTGCTGTTAAGACAATTC+AGG + chr8.2:33519184-33519203 MS.gene056433:CDS 45.0%
GGAGAAGAAAATGAAGGAGG+AGG - chr8.2:33519040-33519059 None:intergenic 45.0%
GTCTCACACTTCTACCCAAA+TGG + chr8.2:33523358-33523377 MS.gene056433:CDS 45.0%
GTGAGTGGGAATGAGTACTA+TGG + chr8.2:33520737-33520756 MS.gene056433:CDS 45.0%
TATGTGGTCAATGCATGGTG+GGG + chr8.2:33522292-33522311 MS.gene056433:intron 45.0%
TCAGGTGAATCATGTCCAGA+AGG + chr8.2:33519563-33519582 MS.gene056433:CDS 45.0%
TGGTGGCTTGAATATGGATC+TGG + chr8.2:33523233-33523252 MS.gene056433:CDS 45.0%
TGTGAGACTACAAGGAGGAT+TGG - chr8.2:33520383-33520402 None:intergenic 45.0%
TTATGCTCTCTTGGTCTCTC+CGG - chr8.2:33519500-33519519 None:intergenic 45.0%
TTTACTATGGTGGTCCTGGA+AGG + chr8.2:33523552-33523571 MS.gene056433:CDS 45.0%
! AGACTACAAGGAGGATTGGT+TGG - chr8.2:33520379-33520398 None:intergenic 45.0%
! AGTGGACATTTTGCTGAGGA+GGG + chr8.2:33523383-33523402 MS.gene056433:CDS 45.0%
! GCGTATTTTACTCACCACCT+TGG + chr8.2:33522834-33522853 MS.gene056433:intron 45.0%
! GGGAATTGGTGGCTTGAATA+TGG + chr8.2:33523227-33523246 MS.gene056433:CDS 45.0%
! GTTTTTCCGCAGAGTCTTGA+TGG + chr8.2:33519288-33519307 MS.gene056433:intron 45.0%
!! TAGTGGACATTTTGCTGAGG+AGG + chr8.2:33523382-33523401 MS.gene056433:CDS 45.0%
!!! TAAATACATTTTTTATTAAT+GGG - chr8.2:33521557-33521576 None:intergenic 5.0%
!!! TTAAATACATTTTTTATTAA+TGG - chr8.2:33521558-33521577 None:intergenic 5.0%
AAATGTGTGGGTCCCACAAG+TGG + chr8.2:33520775-33520794 MS.gene056433:CDS 50.0%
AGCCACCAATTCCCATGCTT+TGG - chr8.2:33523221-33523240 None:intergenic 50.0%
AGCTATCCCCATAGAGCTCT+GGG - chr8.2:33521429-33521448 None:intergenic 50.0%
ATGTGGTCAATGCATGGTGG+GGG + chr8.2:33522293-33522312 MS.gene056433:intron 50.0%
CGTGTTAGAATGGACACCAC+CGG + chr8.2:33519665-33519684 MS.gene056433:CDS 50.0%
GCGTGTAGGATCCAAAGCAT+GGG + chr8.2:33523207-33523226 MS.gene056433:intron 50.0%
TACCATTGAAGCTGGTTGGC+AGG + chr8.2:33520865-33520884 MS.gene056433:CDS 50.0%
TAGCTATCCCCATAGAGCTC+TGG - chr8.2:33521430-33521449 None:intergenic 50.0%
TGCGTGTAGGATCCAAAGCA+TGG + chr8.2:33523206-33523225 MS.gene056433:intron 50.0%
TTGTACCTCATGTCCATCGC+CGG - chr8.2:33519687-33519706 None:intergenic 50.0%
!!! GGGTAGTGGACATTTTGCTG+AGG + chr8.2:33523379-33523398 MS.gene056433:CDS 50.0%
AGGTCAGCCCAGAGCTCTAT+GGG + chr8.2:33521419-33521438 MS.gene056433:intron 55.0%
GAAGGAGGAGGGAGATGACT+GGG - chr8.2:33519028-33519047 None:intergenic 55.0%
TGAAGGAGGAGGGAGATGAC+TGG - chr8.2:33519029-33519048 None:intergenic 55.0%
TGCCTGCCAACCAGCTTCAA+TGG - chr8.2:33520870-33520889 None:intergenic 55.0%
! TCAATGCATGGTGGGGGCAT+GGG + chr8.2:33522299-33522318 MS.gene056433:intron 55.0%
! TTTTTGTGTGCGCGCGCGTT+TGG + chr8.2:33523144-33523163 MS.gene056433:intron 55.0%
AGAATGGACACCACCGGCGA+TGG + chr8.2:33519671-33519690 MS.gene056433:CDS 60.0%
CAGGTCAGCCCAGAGCTCTA+TGG + chr8.2:33521418-33521437 MS.gene056433:intron 60.0%
GGTCAGCCCAGAGCTCTATG+GGG + chr8.2:33521420-33521439 MS.gene056433:intron 60.0%
TACCTCATGTCCATCGCCGG+TGG - chr8.2:33519684-33519703 None:intergenic 60.0%
TGCTCTCTTGGTCTCTCCGG+AGG - chr8.2:33519497-33519516 None:intergenic 60.0%
! CAATGCATGGTGGGGGCATG+GGG + chr8.2:33522300-33522319 MS.gene056433:intron 60.0%
! GTCAATGCATGGTGGGGGCA+TGG + chr8.2:33522298-33522317 MS.gene056433:intron 60.0%
CACCACCGGCGATGGACATG+AGG + chr8.2:33519679-33519698 MS.gene056433:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 33519011 33523592 33519011 ID=MS.gene056433
chr8.2 mRNA 33519011 33523592 33519011 ID=MS.gene056433.t1;Parent=MS.gene056433
chr8.2 exon 33519011 33519205 33519011 ID=MS.gene056433.t1.exon1;Parent=MS.gene056433.t1
chr8.2 CDS 33519011 33519205 33519011 ID=cds.MS.gene056433.t1;Parent=MS.gene056433.t1
chr8.2 exon 33519300 33519386 33519300 ID=MS.gene056433.t1.exon2;Parent=MS.gene056433.t1
chr8.2 CDS 33519300 33519386 33519300 ID=cds.MS.gene056433.t1;Parent=MS.gene056433.t1
chr8.2 exon 33519491 33519700 33519491 ID=MS.gene056433.t1.exon3;Parent=MS.gene056433.t1
chr8.2 CDS 33519491 33519700 33519491 ID=cds.MS.gene056433.t1;Parent=MS.gene056433.t1
chr8.2 exon 33520722 33520886 33520722 ID=MS.gene056433.t1.exon4;Parent=MS.gene056433.t1
chr8.2 CDS 33520722 33520886 33520722 ID=cds.MS.gene056433.t1;Parent=MS.gene056433.t1
chr8.2 exon 33521421 33521474 33521421 ID=MS.gene056433.t1.exon5;Parent=MS.gene056433.t1
chr8.2 CDS 33521421 33521474 33521421 ID=cds.MS.gene056433.t1;Parent=MS.gene056433.t1
chr8.2 exon 33521608 33521754 33521608 ID=MS.gene056433.t1.exon6;Parent=MS.gene056433.t1
chr8.2 CDS 33521608 33521754 33521608 ID=cds.MS.gene056433.t1;Parent=MS.gene056433.t1
chr8.2 exon 33523215 33523592 33523215 ID=MS.gene056433.t1.exon7;Parent=MS.gene056433.t1
chr8.2 CDS 33523215 33523592 33523215 ID=cds.MS.gene056433.t1;Parent=MS.gene056433.t1
Gene Sequence

>MS.gene056433

ATGGATTTCAGCTTCCCAGTCATCTCCCTCCTCCTTCATTTTCTTCTCCTTGTTTCTTTGGTTTGTCATGTTTATTCTTCAGAAACAAGTGGCCAAAATCAAACTTTTCTACCAAATGAAGAGTTTCACAAGTTTAGGAGGACCATAAAAATGCATCTTCGACGGATCAACAAGCCTGCTGTTAAGACAATTCAGGTATGTTAAAAATGTCTTCAAAACATTTCTCAACCTTTTTTAAGAAAAAATAGATGGTGTTTGTGTAATGTGTGTTTGTGTTGTTTTTCCGCAGAGTCTTGATGGTGATATCATAGATTGTGTTATGTCTCATAAACAACCAGCTTTTGATCATCCTATGTTGAAAGGACAAAAACCATTGGTATTTTTCTTAGTTTTTTTTCTCGATCAAAAGGAAATTAAAATTCATAATTTCTTTTATATAATTATATTTCAACTTATAATTGTTCGGTTTTATGTGTATAGGATCCTCCGGAGAGACCAAGAGAGCATAAGCAAATGGGCATTGAAAATTATAACTTTCAATTGTGGAGTTTCTCAGGTGAATCATGTCCAGAAGGAACGATTCCGATTAGAAGAATAAAAGAAGATGACATGTTAAGAGCTTGCTCTATTAACACATTTGGAAGAAAATTGAAACGTGTTAGAATGGACACCACCGGCGATGGACATGAGGTACAATGCCATCACAGAAAACAACAGAAACTCAGATTATTTTGTCTCATAATTTCTTTTATATTATATACTTTTATGTATCTTTTCATTATGGTAAAGTATGGAATAAATAAACAACTTAAGAGATAACCCAAAAAACAACTTAACATAGATAGATAATGGAAATTGGTAAAGCATATGGTAGTATTTTTTGAGGGTAAAGCATATGGTAGTTGAGTATTTAATTTTACGTTAAACGTGTTTTGGCTTATATAAAATTACGGTGGTAAGTTTAGTCTTTATAGCCTTCCAAAAGAAAAGTTAGTCTCCGTAAAAGAAATTTGATCATTTTTGTCTATATTTTCTTTATAAGAACTTGTGATTTTAGCTCCTACATAAGGACTAAAGTATGTATTAAATTTTATAAAAAGTAAAGATTAAAAATTGTTCAAAAAGTTTTATTTTTTTGAAACAGTTCAAAAAGAATTTATAAAGACTAAAACAATTTTTTTATATTTTATAAGTTACAAAAACATATGTATCCATTCATTTCTTTTGAAATAATTAATATCGGTCATAAAAGAGAAATTCCAAATGTTTTCTTTTTCTTTTTATACTATATTTTTTAAAAAATTTATAAGTATGAAAATGAAGATAGTACGATTAGCACAAAAAAATTGAAAATGATCTGTAGTACCAACCAATCCTCCTTGTAGTCTCACATTAATACAAACAAAATAATATTATGATAACTTCTTCCAAAAATAATATTCTGATAAAAGCATAATAGACAAGTGTTCTTTTTTTTTTTTTTTGTCTTTCTAATTTACTCATGAAGCTAAGTCCTTTTTCAATTTAAAGTGGTCACTGAATTTCATGGTTACTCACTTCTCAGTTTATTAATAGGTGGACCAATTATTTTTATCTTCAATAGAAATAATCTTAGACACAACAAAAACCAACATTGAATCTAATATAAATCTTGTGCTATTTTTGAAAATAAAGTACTAACAACAAATTGGTTCTTCTTAATGATGCACAGCATGCAGTTGGATATGTGAGTGGGAATGAGTACTATGGAGCAAAAGCTAGCATAAATGTGTGGGTCCCACAAGTGGAAAGTCCATATGAATTCAGCTTGTCTCAAATATGGATCATCTCTGGTTCATTTGGAGTAGATCTCAATACCATTGAAGCTGGTTGGCAGGCATGTTGATTATTTTCATTAATTACTTCATCTTAGACAATCAATTCATTCAATCACTTCTTTTGCACTGCTTTTTTTAGTAGGTACTCCCACTCATTTTTTACTTGTCATTTTAAGGTTACGTGTATTTCATACGTCTCAAAATAAGTGACATTGTAGACAGTTGCACATAGATATTTTTGACATTTAATATTTTTGATTATGTATTAGTAAAAATTATAAATGCAAAACCAAATCCAATACTTAATAATGATACTTTAATAAAAATGATGTTATCTCTTTTCCATTTATCCATTTTTCTTGATATGTATAAAATGAACAAATAGAACAATTAATTTGGAAAGAAGGGAGTATATTTTATTAATCTTTTACACAATTTTTTTAAACTAATTTTACACGAAAATTCTTAAATTGATTGGATTTGGTTTTGCATTAAATAGGCAGTTAGAATGTCAACCAACTACCTTTCATTTTCATCAATGTTTTTAATTTTGTATTGGTCAATCAAGCTAAGTTTAAGTAATTTTTATGCAGGTCAGCCCAGAGCTCTATGGGGATAGCTACCCTAGATTCTTCACTTATTGGACGGTAAGTAAATTGATTGAAGCAAGGGAGGTGGAAAAGTAAAATATCTATGATTGATACTACTTGCTTCAAGTGTAAGATACCCATTAATAAAAAATGTATTTAAAAAAATGATAATGGCTAGTAATAATTGCAGACTGATGCATATCAAGGAACTGGGTGCTACAATTTACTTTGCTCTGGCTTTGTTCAAACTAGCAGTAATATTGCAATTGGAGCAGCAATTTCTCCAACTTCTACATATAATGGAGGCCAATTTGATATTAGCTTAATGATCTGGAAGGTAATTTTCTGCTCAGAATACCAAAATGACCTTAGAAGATTATGGGTAATTTGGTATATATCCCTCCAAGTTTCTACTGGTATTTACTACTCCTCCGATCACATTTATAATCAATTTTTTTTTAAAATTTATTAAATAACTGATGTATCTAGATTATAATATAGTCTGGATACATCCGTTATTTAATGAATGTGAAAAATTAAAAACTGTTTATAAATGTGGCCCGATGGAGTACTTATTTTCAATAATAATACAGCTTCAAATATGTAAAATAAGTCAATTTAGTTACTAAAGTTAAAACAAAATAAAATAGTTCTTCGAAATAAAAAAATTTCAATTAATTAATTATAATTAATTATTCCAGTGCCAATTTAGTCAATGAGATTATTGCATATTGACAAATGTGATTAGAAACTTTTAGTTTTAAAGAACATCTGAATATTTTTTTAACTTCAATAACCAAATTAACTAACCATTGATTTGTTACAAAATATTGAAGTCTCATAGTCAAAGTCTCAAAGGAAAATTTAAGCTGAATATGTGGTCAATGCATGGTGGGGGCATGGGGCACTCCATTAGTTTGAAAGTCTCATTATATGCTTTCGAATTTTGTTATTATGTAGAATTGTAGAAGAAGCAAGGGCAAGTTTATTATAACATGTTCACAACATCATAATTTGTTTTGAAAAAAGCAACATAAATGACACACAAAAGGAGAAAAACAATATAATGAACAAAATTTTCTAGTGTTCAAATGTTAGTGAATTTAGGTACCATATTTACTCACTTTACTACTTTGGTACATTACACGCATGAACTGTGTAAATATGAATGTAACTCCCTTATGCTTTTTATCTCTTTCAAGATTCTTTTTCTTTATTCTTTTTTCTTTCCCTTTTTTGTTTCTTTTTTTCACCACATAATTTATCATATTTTTTACTTTCTCTCATTCTTTCTGAGCACTTTGAGAACATCATTTTCCTTTGCTTTTTACGACTTAAAGATATTCCATAATTCTGAACGCCACACCTTTTTTTATTCCTTCATTTTTCTCTTATTTAACAAAACCCTAATATTTTGCGTATTTTACTCACCACCTTGGTACCTTTGTTTTCTTCTTTTTGACTCATTTCTATTATCATGTCAGCTAACATGCTGCTCTTAATGATTATGTTTATGTTTGAGTAATTTAACTTTGTCACAAAACAAAAGTTGTATCGTTGAAGATTTACTAGGGTAACATTGATACCTCAGATTGAATGTGTGTCAGATGATTAACATATGCTTGTGTTCGATGTGGTTATATTAATTCAGTTACTTCTATTTTTCTTTTTTAAACTATTAAAAAGGTTTATATCACAATGTTAGTGTTGTGTATGGTATTTTTTTTTTTTGTGTGCGCGCGCGTTTGGTGTTTGTGTTTCATATATAGTTTACTTTGCTTCTATTTATGCGTGTAGGATCCAAAGCATGGGAATTGGTGGCTTGAATATGGATCTGGAAACTTGATTGGGTATTGGCCATCATCCCTATTCACACACTTAAAGGATAGTGCAACAATGGTTCAATTTGGTGGAGAAATAGTGAATACAATGTCAACAGGGTCTCACACTTCTACCCAAATGGGTAGTGGACATTTTGCTGAGGAGGGTTTTGCAAAAGCTGCATACTTTAGAAATATGCAAGTTGTGGATTCTGATAACAGCTTGATTCCCTTGCCAAATCTCAATGTTGTAGCTAACCGTCCAAATTGTTATAATATACAAGAAGGAACTAATAATGGGTGGGGAAATTACTTTTACTATGGTGGTCCTGGAAGGAATGTAAAATGTCCCTAA
Protein sequence

>MS.gene056433.t1

MDFSFPVISLLLHFLLLVSLVCHVYSSETSGQNQTFLPNEEFHKFRRTIKMHLRRINKPAVKTIQSLDGDIIDCVMSHKQPAFDHPMLKGQKPLDPPERPREHKQMGIENYNFQLWSFSGESCPEGTIPIRRIKEDDMLRACSINTFGRKLKRVRMDTTGDGHEHAVGYVSGNEYYGAKASINVWVPQVESPYEFSLSQIWIISGSFGVDLNTIEAGWQVSPELYGDSYPRFFTYWTTDAYQGTGCYNLLCSGFVQTSSNIAIGAAISPTSTYNGGQFDISLMIWKDPKHGNWWLEYGSGNLIGYWPSSLFTHLKDSATMVQFGGEIVNTMSTGSHTSTQMGSGHFAEEGFAKAAYFRNMQVVDSDNSLIPLPNLNVVANRPNCYNIQEGTNNGWGNYFYYGGPGRNVKCP