Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056467.t1 | XP_013446194.1 | 99.4 | 158 | 1 | 0 | 1 | 158 | 596 | 753 | 2.30E-80 | 308.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056467.t1 | Q9SX33 | 81.6 | 158 | 29 | 0 | 1 | 158 | 590 | 747 | 6.5e-68 | 258.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056467.t1 | A0A072TSN7 | 99.4 | 158 | 1 | 0 | 1 | 158 | 596 | 753 | 1.7e-80 | 308.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051279 | MS.gene056467 | 0.808757 | 2.65E-50 | -1.69E-46 |
| MS.gene056467 | MS.gene05686 | 0.807758 | 4.32E-50 | -1.69E-46 |
| MS.gene056467 | MS.gene063691 | 0.803921 | 2.77E-49 | -1.69E-46 |
| MS.gene056467 | MS.gene074625 | 0.893608 | 5.37E-75 | -1.69E-46 |
| MS.gene056467 | MS.gene09600 | 0.801332 | 9.47E-49 | -1.69E-46 |
| MS.gene056467 | MS.gene20298 | 0.802788 | 4.75E-49 | -1.69E-46 |
| MS.gene056467 | MS.gene35324 | 0.805588 | 1.24E-49 | -1.69E-46 |
| MS.gene056467 | MS.gene58571 | 0.800358 | 1.50E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGAAACTGCAATCAATAT+TGG | 0.224848 | 8.3:-32666469 | MS.gene056467:CDS |
| CCCAAGCAGGAATTAAGATT+TGG | 0.277141 | 8.3:-32666510 | MS.gene056467:CDS |
| GAGAAGAACCTAATCCTTCT+TGG | 0.288851 | 8.3:-32666686 | MS.gene056467:CDS |
| GTTCACGATAGGCGAGTATA+AGG | 0.289003 | 8.3:+32666813 | None:intergenic |
| ATCTGCACTAACTAAATTCT+TGG | 0.320331 | 8.3:+32666745 | None:intergenic |
| CCAAATCTTAATTCCTGCTT+GGG | 0.383541 | 8.3:+32666510 | None:intergenic |
| CCAAGCAGGAATTAAGATTT+GGG | 0.387145 | 8.3:-32666509 | MS.gene056467:CDS |
| TGGAAACTGCAATCAATATT+GGG | 0.402010 | 8.3:-32666468 | None:intergenic |
| CCCAAATCTTAATTCCTGCT+TGG | 0.405617 | 8.3:+32666509 | None:intergenic |
| TTTGAAAGACTTGCTAAGAA+TGG | 0.415716 | 8.3:-32666899 | MS.gene056467:CDS |
| TATTCTAATTCACCCCATCT+TGG | 0.419173 | 8.3:+32666639 | None:intergenic |
| CAGTGGCACCAAGAAGGATT+AGG | 0.446298 | 8.3:+32666678 | None:intergenic |
| GAATTTAGTTAGTGCAGATC+GGG | 0.459507 | 8.3:-32666741 | MS.gene056467:CDS |
| GCAAGCTTGTCAATGCAATC+AGG | 0.489298 | 8.3:+32666532 | None:intergenic |
| AGAATTTAGTTAGTGCAGAT+CGG | 0.501336 | 8.3:-32666742 | MS.gene056467:CDS |
| GTGCATGAATACGCCGATGC+AGG | 0.509361 | 8.3:-32666845 | MS.gene056467:CDS |
| TCTTGGTGCCACTGCTGTAG+AGG | 0.514580 | 8.3:-32666669 | MS.gene056467:CDS |
| AAAGACTTGCTAAGAATGGA+AGG | 0.521525 | 8.3:-32666895 | MS.gene056467:CDS |
| TAGAGGACAAGCTCCAAGAT+GGG | 0.529175 | 8.3:-32666652 | MS.gene056467:CDS |
| GTATTCATGCACATGTTCCA+TGG | 0.530981 | 8.3:+32666856 | None:intergenic |
| ATTGACAAGCTTGCCCAAGC+AGG | 0.535786 | 8.3:-32666523 | MS.gene056467:CDS |
| TTCACGATAGGCGAGTATAA+GGG | 0.550147 | 8.3:+32666814 | None:intergenic |
| CTATCGTGAACTTGCAGCAG+AGG | 0.553461 | 8.3:-32666801 | MS.gene056467:CDS |
| GTAATAGTACAAGATGGTGA+AGG | 0.566634 | 8.3:-32667062 | MS.gene056467:CDS |
| GTAGAGGACAAGCTCCAAGA+TGG | 0.567052 | 8.3:-32666653 | MS.gene056467:CDS |
| AGATCGGGAAACACTGATTG+AGG | 0.585749 | 8.3:-32666726 | MS.gene056467:CDS |
| AAGGGTTCTTAGACCTGCAT+CGG | 0.586916 | 8.3:+32666832 | None:intergenic |
| TAATAGTACAAGATGGTGAA+GGG | 0.587286 | 8.3:-32667061 | MS.gene056467:CDS |
| CTACAGCAGTGGCACCAAGA+AGG | 0.595105 | 8.3:+32666672 | None:intergenic |
| TACTCTGCAAAGGTGCCGAT+AGG | 0.596972 | 8.3:-32667028 | MS.gene056467:intron |
| GAGCTTGTCCTCTACAGCAG+TGG | 0.599640 | 8.3:+32666661 | None:intergenic |
| CTCTGCTGCAAGTTCACGAT+AGG | 0.607696 | 8.3:+32666802 | None:intergenic |
| TAGCTCAAGGAAGAGAATGT+CGG | 0.630448 | 8.3:-32667084 | MS.gene056467:CDS |
| GGAGTTTGAAGAGAAAACCA+TGG | 0.635970 | 8.3:-32666873 | MS.gene056467:CDS |
| ATGTCGGTAATAGTACAAGA+TGG | 0.639491 | 8.3:-32667068 | MS.gene056467:CDS |
| AAGACTTGCTAAGAATGGAA+GGG | 0.650987 | 8.3:-32666894 | MS.gene056467:CDS |
| AGAGGACAAGCTCCAAGATG+GGG | 0.671294 | 8.3:-32666651 | MS.gene056467:intron |
| GTTTGATAACACATTCTCTG+AGG | 0.696265 | 8.3:-32666768 | MS.gene056467:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACATTCAAAATCATTTAA+GGG | + | chr8.3:32666605-32666624 | None:intergenic | 15.0% |
| !! | TAAACATTCAAAATCATTTA+AGG | + | chr8.3:32666606-32666625 | None:intergenic | 15.0% |
| !! | TTAGTTATGAAGATTATTAT+TGG | - | chr8.3:32666985-32667004 | MS.gene056467:intron | 15.0% |
| !! | TATGAAGATTATTATTGGAA+CGG | - | chr8.3:32666990-32667009 | MS.gene056467:intron | 20.0% |
| !!! | AAAATTTTATTACTCTGCAA+AGG | - | chr8.3:32666548-32666567 | MS.gene056467:intron | 20.0% |
| ! | ACTGCATGAAATATCAATTA+AGG | + | chr8.3:32666661-32666680 | None:intergenic | 25.0% |
| ! | ATGAAGATTATTATTGGAAC+GGG | - | chr8.3:32666991-32667010 | MS.gene056467:intron | 25.0% |
| !!! | TCTTTTGTACTTTTTTTGCA+AGG | - | chr8.3:32667029-32667048 | MS.gene056467:CDS | 25.0% |
| !!! | TTTTAGAATTCAATAGCTCA+AGG | - | chr8.3:32666489-32666508 | MS.gene056467:CDS | 25.0% |
| AGAATTTAGTTAGTGCAGAT+CGG | - | chr8.3:32666844-32666863 | MS.gene056467:CDS | 30.0% | |
| ATCTGCACTAACTAAATTCT+TGG | + | chr8.3:32666844-32666863 | None:intergenic | 30.0% | |
| ATGGAAACTGCAATCAATAT+TGG | - | chr8.3:32667117-32667136 | MS.gene056467:CDS | 30.0% | |
| CATGAAATATCAATTAAGGC+TGG | + | chr8.3:32666657-32666676 | None:intergenic | 30.0% | |
| GGATAATGAGTGAAAAACAA+AGG | + | chr8.3:32666636-32666655 | None:intergenic | 30.0% | |
| TAATAGTACAAGATGGTGAA+GGG | - | chr8.3:32666525-32666544 | MS.gene056467:CDS | 30.0% | |
| TCGATTTATCACAAACCTAT+CGG | + | chr8.3:32666576-32666595 | None:intergenic | 30.0% | |
| TGGAAACTGCAATCAATATT+GGG | - | chr8.3:32667118-32667137 | MS.gene056467:CDS | 30.0% | |
| !! | ATTAAGATTTGGGTTTTGAC+TGG | - | chr8.3:32667087-32667106 | MS.gene056467:CDS | 30.0% |
| !! | TTTGAAAGACTTGCTAAGAA+TGG | - | chr8.3:32666687-32666706 | MS.gene056467:CDS | 30.0% |
| !!! | TTAAGATTTGGGTTTTGACT+GGG | - | chr8.3:32667088-32667107 | MS.gene056467:CDS | 30.0% |
| ATGTCGGTAATAGTACAAGA+TGG | - | chr8.3:32666518-32666537 | MS.gene056467:CDS | 35.0% | |
| CCAAATCTTAATTCCTGCTT+GGG | + | chr8.3:32667079-32667098 | None:intergenic | 35.0% | |
| CCAAGCAGGAATTAAGATTT+GGG | - | chr8.3:32667077-32667096 | MS.gene056467:CDS | 35.0% | |
| GAATTTAGTTAGTGCAGATC+GGG | - | chr8.3:32666845-32666864 | MS.gene056467:CDS | 35.0% | |
| GTAATAGTACAAGATGGTGA+AGG | - | chr8.3:32666524-32666543 | MS.gene056467:CDS | 35.0% | |
| TATTCTAATTCACCCCATCT+TGG | + | chr8.3:32666950-32666969 | None:intergenic | 35.0% | |
| ! | GTTTGATAACACATTCTCTG+AGG | - | chr8.3:32666818-32666837 | MS.gene056467:CDS | 35.0% |
| !! | AAAGACTTGCTAAGAATGGA+AGG | - | chr8.3:32666691-32666710 | MS.gene056467:CDS | 35.0% |
| !! | AAGACTTGCTAAGAATGGAA+GGG | - | chr8.3:32666692-32666711 | MS.gene056467:CDS | 35.0% |
| !!! | TAAGATTTGGGTTTTGACTG+GGG | - | chr8.3:32667089-32667108 | MS.gene056467:CDS | 35.0% |
| CCCAAATCTTAATTCCTGCT+TGG | + | chr8.3:32667080-32667099 | None:intergenic | 40.0% | |
| CCCAAGCAGGAATTAAGATT+TGG | - | chr8.3:32667076-32667095 | MS.gene056467:CDS | 40.0% | |
| GAGAAGAACCTAATCCTTCT+TGG | - | chr8.3:32666900-32666919 | MS.gene056467:CDS | 40.0% | |
| GGAGTTTGAAGAGAAAACCA+TGG | - | chr8.3:32666713-32666732 | MS.gene056467:CDS | 40.0% | |
| GTATTCATGCACATGTTCCA+TGG | + | chr8.3:32666733-32666752 | None:intergenic | 40.0% | |
| TAGCTCAAGGAAGAGAATGT+CGG | - | chr8.3:32666502-32666521 | MS.gene056467:CDS | 40.0% | |
| TTCACGATAGGCGAGTATAA+GGG | + | chr8.3:32666775-32666794 | None:intergenic | 40.0% | |
| !!! | GGTTTTGACTGGGGATAAAA+TGG | - | chr8.3:32667098-32667117 | MS.gene056467:CDS | 40.0% |
| AAGGGTTCTTAGACCTGCAT+CGG | + | chr8.3:32666757-32666776 | None:intergenic | 45.0% | |
| GCAAGCTTGTCAATGCAATC+AGG | + | chr8.3:32667057-32667076 | None:intergenic | 45.0% | |
| GTTCACGATAGGCGAGTATA+AGG | + | chr8.3:32666776-32666795 | None:intergenic | 45.0% | |
| TAGAGGACAAGCTCCAAGAT+GGG | - | chr8.3:32666934-32666953 | MS.gene056467:intron | 45.0% | |
| ! | AGATCGGGAAACACTGATTG+AGG | - | chr8.3:32666860-32666879 | MS.gene056467:CDS | 45.0% |
| ! | TGGAACGGGACTAGTAATCA+TGG | - | chr8.3:32667005-32667024 | MS.gene056467:intron | 45.0% |
| AGAGGACAAGCTCCAAGATG+GGG | - | chr8.3:32666935-32666954 | MS.gene056467:intron | 50.0% | |
| ATTGACAAGCTTGCCCAAGC+AGG | - | chr8.3:32667063-32667082 | MS.gene056467:CDS | 50.0% | |
| CAGTGGCACCAAGAAGGATT+AGG | + | chr8.3:32666911-32666930 | None:intergenic | 50.0% | |
| CTATCGTGAACTTGCAGCAG+AGG | - | chr8.3:32666785-32666804 | MS.gene056467:CDS | 50.0% | |
| CTCTGCTGCAAGTTCACGAT+AGG | + | chr8.3:32666787-32666806 | None:intergenic | 50.0% | |
| GTAGAGGACAAGCTCCAAGA+TGG | - | chr8.3:32666933-32666952 | MS.gene056467:intron | 50.0% | |
| !! | TACTCTGCAAAGGTGCCGAT+AGG | - | chr8.3:32666558-32666577 | MS.gene056467:intron | 50.0% |
| CTACAGCAGTGGCACCAAGA+AGG | + | chr8.3:32666917-32666936 | None:intergenic | 55.0% | |
| GAGCTTGTCCTCTACAGCAG+TGG | + | chr8.3:32666928-32666947 | None:intergenic | 55.0% | |
| GTGCATGAATACGCCGATGC+AGG | - | chr8.3:32666741-32666760 | MS.gene056467:CDS | 55.0% | |
| !! | TCTTGGTGCCACTGCTGTAG+AGG | - | chr8.3:32666917-32666936 | MS.gene056467:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 32666469 | 32667139 | 32666469 | ID=MS.gene056467 |
| chr8.3 | mRNA | 32666469 | 32667139 | 32666469 | ID=MS.gene056467.t1;Parent=MS.gene056467 |
| chr8.3 | exon | 32667029 | 32667139 | 32667029 | ID=MS.gene056467.t1.exon1;Parent=MS.gene056467.t1 |
| chr8.3 | CDS | 32667029 | 32667139 | 32667029 | ID=cds.MS.gene056467.t1;Parent=MS.gene056467.t1 |
| chr8.3 | exon | 32666652 | 32666928 | 32666652 | ID=MS.gene056467.t1.exon2;Parent=MS.gene056467.t1 |
| chr8.3 | CDS | 32666652 | 32666928 | 32666652 | ID=cds.MS.gene056467.t1;Parent=MS.gene056467.t1 |
| chr8.3 | exon | 32666469 | 32666557 | 32666469 | ID=MS.gene056467.t1.exon3;Parent=MS.gene056467.t1 |
| chr8.3 | CDS | 32666469 | 32666557 | 32666469 | ID=cds.MS.gene056467.t1;Parent=MS.gene056467.t1 |
| Gene Sequence |
| Protein sequence |
