Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074625.t1 | XP_013446194.1 | 99.4 | 158 | 1 | 0 | 1 | 158 | 596 | 753 | 2.30E-80 | 308.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074625.t1 | Q9SX33 | 81.6 | 158 | 29 | 0 | 1 | 158 | 590 | 747 | 6.5e-68 | 258.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074625.t1 | A0A072TSN7 | 99.4 | 158 | 1 | 0 | 1 | 158 | 596 | 753 | 1.7e-80 | 308.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051279 | MS.gene074625 | 0.80171 | 7.92E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene074625 | 0.803859 | 2.85E-49 | -1.69E-46 |
| MS.gene056467 | MS.gene074625 | 0.893608 | 5.37E-75 | -1.69E-46 |
| MS.gene056468 | MS.gene074625 | 0.822483 | 2.31E-53 | -1.69E-46 |
| MS.gene05686 | MS.gene074625 | 0.80319 | 3.92E-49 | -1.69E-46 |
| MS.gene060353 | MS.gene074625 | 0.804284 | 2.33E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGAAACTGCAATCAATAT+TGG | 0.224848 | 8.3:+32665722 | MS.gene074625:CDS |
| CCCAAGCAGGAATTAAGATT+TGG | 0.277141 | 8.3:+32665681 | MS.gene074625:CDS |
| GAGAAGAACCTAATCCTTCT+TGG | 0.288851 | 8.3:+32665505 | MS.gene074625:CDS |
| GTTCACGATAGGCGAGTATA+AGG | 0.289003 | 8.3:-32665378 | None:intergenic |
| ATCTGCACTAACTAAATTCT+TGG | 0.320331 | 8.3:-32665446 | None:intergenic |
| CCAAATCTTAATTCCTGCTT+GGG | 0.383541 | 8.3:-32665681 | None:intergenic |
| CCAAGCAGGAATTAAGATTT+GGG | 0.387145 | 8.3:+32665682 | MS.gene074625:CDS |
| TGGAAACTGCAATCAATATT+GGG | 0.402010 | 8.3:+32665723 | MS.gene074625:CDS |
| CCCAAATCTTAATTCCTGCT+TGG | 0.405617 | 8.3:-32665682 | None:intergenic |
| TTTGAAAGACTTGCTAAGAA+TGG | 0.415716 | 8.3:+32665292 | MS.gene074625:CDS |
| TATTCTAATTCACCCCATCT+TGG | 0.419173 | 8.3:-32665552 | None:intergenic |
| CAGTGGCACCAAGAAGGATT+AGG | 0.446298 | 8.3:-32665513 | None:intergenic |
| GAATTTAGTTAGTGCAGATC+GGG | 0.459507 | 8.3:+32665450 | MS.gene074625:CDS |
| GCAAGCTTGTCAATGCAATC+AGG | 0.489298 | 8.3:-32665659 | None:intergenic |
| AGAATTTAGTTAGTGCAGAT+CGG | 0.501336 | 8.3:+32665449 | MS.gene074625:CDS |
| GTGCATGAATACGCCGATGC+AGG | 0.509361 | 8.3:+32665346 | MS.gene074625:CDS |
| TCTTGGTGCCACTGCTGTAG+AGG | 0.514580 | 8.3:+32665522 | MS.gene074625:CDS |
| AAAGACTTGCTAAGAATGGA+AGG | 0.521525 | 8.3:+32665296 | MS.gene074625:CDS |
| TAGAGGACAAGCTCCAAGAT+GGG | 0.529175 | 8.3:+32665539 | MS.gene074625:CDS |
| GTATTCATGCACATGTTCCA+TGG | 0.530981 | 8.3:-32665335 | None:intergenic |
| ATTGACAAGCTTGCCCAAGC+AGG | 0.535786 | 8.3:+32665668 | MS.gene074625:CDS |
| TTCACGATAGGCGAGTATAA+GGG | 0.550147 | 8.3:-32665377 | None:intergenic |
| CTATCGTGAACTTGCAGCAG+AGG | 0.553461 | 8.3:+32665390 | MS.gene074625:CDS |
| GTAATAGTACAAGATGGTGA+AGG | 0.566634 | 8.3:+32665129 | MS.gene074625:CDS |
| GTAGAGGACAAGCTCCAAGA+TGG | 0.567052 | 8.3:+32665538 | MS.gene074625:CDS |
| AGATCGGGAAACACTGATTG+AGG | 0.585749 | 8.3:+32665465 | MS.gene074625:CDS |
| AAGGGTTCTTAGACCTGCAT+CGG | 0.586916 | 8.3:-32665359 | None:intergenic |
| TAATAGTACAAGATGGTGAA+GGG | 0.587286 | 8.3:+32665130 | MS.gene074625:CDS |
| CTACAGCAGTGGCACCAAGA+AGG | 0.595105 | 8.3:-32665519 | None:intergenic |
| TACTCTGCAAAGGTGCCGAT+AGG | 0.596972 | 8.3:+32665163 | MS.gene074625:CDS |
| GAGCTTGTCCTCTACAGCAG+TGG | 0.599640 | 8.3:-32665530 | None:intergenic |
| CTCTGCTGCAAGTTCACGAT+AGG | 0.607696 | 8.3:-32665389 | None:intergenic |
| TAGCTCAAGGAAGAGAATGT+CGG | 0.630448 | 8.3:+32665107 | MS.gene074625:CDS |
| GGAGTTTGAAGAGAAAACCA+TGG | 0.635970 | 8.3:+32665318 | MS.gene074625:CDS |
| ATGTCGGTAATAGTACAAGA+TGG | 0.639491 | 8.3:+32665123 | MS.gene074625:CDS |
| AAGACTTGCTAAGAATGGAA+GGG | 0.650987 | 8.3:+32665297 | MS.gene074625:CDS |
| AGAGGACAAGCTCCAAGATG+GGG | 0.671294 | 8.3:+32665540 | MS.gene074625:CDS |
| GTTTGATAACACATTCTCTG+AGG | 0.696265 | 8.3:+32665423 | MS.gene074625:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACATTCAAAATCATTTAA+GGG | - | chr8.3:32665210-32665229 | None:intergenic | 15.0% |
| !! | TAAACATTCAAAATCATTTA+AGG | - | chr8.3:32665211-32665230 | None:intergenic | 15.0% |
| !! | TTAGTTATGAAGATTATTAT+TGG | + | chr8.3:32665590-32665609 | MS.gene074625:intron | 15.0% |
| !! | TATGAAGATTATTATTGGAA+CGG | + | chr8.3:32665595-32665614 | MS.gene074625:intron | 20.0% |
| !!! | AAAATTTTATTACTCTGCAA+AGG | + | chr8.3:32665153-32665172 | MS.gene074625:CDS | 20.0% |
| ! | ACTGCATGAAATATCAATTA+AGG | - | chr8.3:32665266-32665285 | None:intergenic | 25.0% |
| ! | ATGAAGATTATTATTGGAAC+GGG | + | chr8.3:32665596-32665615 | MS.gene074625:intron | 25.0% |
| !!! | TCTTTTGTACTTTTTTTGCA+AGG | + | chr8.3:32665634-32665653 | MS.gene074625:intron | 25.0% |
| !!! | TTTTAGAATTCAATAGCTCA+AGG | + | chr8.3:32665094-32665113 | MS.gene074625:CDS | 25.0% |
| AGAATTTAGTTAGTGCAGAT+CGG | + | chr8.3:32665449-32665468 | MS.gene074625:CDS | 30.0% | |
| ATCTGCACTAACTAAATTCT+TGG | - | chr8.3:32665449-32665468 | None:intergenic | 30.0% | |
| ATGGAAACTGCAATCAATAT+TGG | + | chr8.3:32665722-32665741 | MS.gene074625:CDS | 30.0% | |
| CATGAAATATCAATTAAGGC+TGG | - | chr8.3:32665262-32665281 | None:intergenic | 30.0% | |
| GGATAATGAGTGAAAAACAA+AGG | - | chr8.3:32665241-32665260 | None:intergenic | 30.0% | |
| TAATAGTACAAGATGGTGAA+GGG | + | chr8.3:32665130-32665149 | MS.gene074625:CDS | 30.0% | |
| TCGATTTATCACAAACCTAT+CGG | - | chr8.3:32665181-32665200 | None:intergenic | 30.0% | |
| TGGAAACTGCAATCAATATT+GGG | + | chr8.3:32665723-32665742 | MS.gene074625:CDS | 30.0% | |
| !! | ATTAAGATTTGGGTTTTGAC+TGG | + | chr8.3:32665692-32665711 | MS.gene074625:CDS | 30.0% |
| !! | TTTGAAAGACTTGCTAAGAA+TGG | + | chr8.3:32665292-32665311 | MS.gene074625:CDS | 30.0% |
| !!! | TTAAGATTTGGGTTTTGACT+GGG | + | chr8.3:32665693-32665712 | MS.gene074625:CDS | 30.0% |
| ATGTCGGTAATAGTACAAGA+TGG | + | chr8.3:32665123-32665142 | MS.gene074625:CDS | 35.0% | |
| CCAAATCTTAATTCCTGCTT+GGG | - | chr8.3:32665684-32665703 | None:intergenic | 35.0% | |
| CCAAGCAGGAATTAAGATTT+GGG | + | chr8.3:32665682-32665701 | MS.gene074625:CDS | 35.0% | |
| GAATTTAGTTAGTGCAGATC+GGG | + | chr8.3:32665450-32665469 | MS.gene074625:CDS | 35.0% | |
| GTAATAGTACAAGATGGTGA+AGG | + | chr8.3:32665129-32665148 | MS.gene074625:CDS | 35.0% | |
| TATTCTAATTCACCCCATCT+TGG | - | chr8.3:32665555-32665574 | None:intergenic | 35.0% | |
| ! | GTTTGATAACACATTCTCTG+AGG | + | chr8.3:32665423-32665442 | MS.gene074625:CDS | 35.0% |
| !! | AAAGACTTGCTAAGAATGGA+AGG | + | chr8.3:32665296-32665315 | MS.gene074625:CDS | 35.0% |
| !! | AAGACTTGCTAAGAATGGAA+GGG | + | chr8.3:32665297-32665316 | MS.gene074625:CDS | 35.0% |
| !!! | TAAGATTTGGGTTTTGACTG+GGG | + | chr8.3:32665694-32665713 | MS.gene074625:CDS | 35.0% |
| CCCAAATCTTAATTCCTGCT+TGG | - | chr8.3:32665685-32665704 | None:intergenic | 40.0% | |
| CCCAAGCAGGAATTAAGATT+TGG | + | chr8.3:32665681-32665700 | MS.gene074625:CDS | 40.0% | |
| GAGAAGAACCTAATCCTTCT+TGG | + | chr8.3:32665505-32665524 | MS.gene074625:CDS | 40.0% | |
| GGAGTTTGAAGAGAAAACCA+TGG | + | chr8.3:32665318-32665337 | MS.gene074625:CDS | 40.0% | |
| GTATTCATGCACATGTTCCA+TGG | - | chr8.3:32665338-32665357 | None:intergenic | 40.0% | |
| TAGCTCAAGGAAGAGAATGT+CGG | + | chr8.3:32665107-32665126 | MS.gene074625:CDS | 40.0% | |
| TTCACGATAGGCGAGTATAA+GGG | - | chr8.3:32665380-32665399 | None:intergenic | 40.0% | |
| !!! | GGTTTTGACTGGGGATAAAA+TGG | + | chr8.3:32665703-32665722 | MS.gene074625:CDS | 40.0% |
| AAGGGTTCTTAGACCTGCAT+CGG | - | chr8.3:32665362-32665381 | None:intergenic | 45.0% | |
| GCAAGCTTGTCAATGCAATC+AGG | - | chr8.3:32665662-32665681 | None:intergenic | 45.0% | |
| GTTCACGATAGGCGAGTATA+AGG | - | chr8.3:32665381-32665400 | None:intergenic | 45.0% | |
| TAGAGGACAAGCTCCAAGAT+GGG | + | chr8.3:32665539-32665558 | MS.gene074625:CDS | 45.0% | |
| ! | AGATCGGGAAACACTGATTG+AGG | + | chr8.3:32665465-32665484 | MS.gene074625:CDS | 45.0% |
| ! | TGGAACGGGACTAGTAATCA+TGG | + | chr8.3:32665610-32665629 | MS.gene074625:intron | 45.0% |
| AGAGGACAAGCTCCAAGATG+GGG | + | chr8.3:32665540-32665559 | MS.gene074625:CDS | 50.0% | |
| ATTGACAAGCTTGCCCAAGC+AGG | + | chr8.3:32665668-32665687 | MS.gene074625:CDS | 50.0% | |
| CAGTGGCACCAAGAAGGATT+AGG | - | chr8.3:32665516-32665535 | None:intergenic | 50.0% | |
| CTATCGTGAACTTGCAGCAG+AGG | + | chr8.3:32665390-32665409 | MS.gene074625:CDS | 50.0% | |
| CTCTGCTGCAAGTTCACGAT+AGG | - | chr8.3:32665392-32665411 | None:intergenic | 50.0% | |
| GTAGAGGACAAGCTCCAAGA+TGG | + | chr8.3:32665538-32665557 | MS.gene074625:CDS | 50.0% | |
| !! | TACTCTGCAAAGGTGCCGAT+AGG | + | chr8.3:32665163-32665182 | MS.gene074625:CDS | 50.0% |
| CTACAGCAGTGGCACCAAGA+AGG | - | chr8.3:32665522-32665541 | None:intergenic | 55.0% | |
| GAGCTTGTCCTCTACAGCAG+TGG | - | chr8.3:32665533-32665552 | None:intergenic | 55.0% | |
| GTGCATGAATACGCCGATGC+AGG | + | chr8.3:32665346-32665365 | MS.gene074625:CDS | 55.0% | |
| !! | TCTTGGTGCCACTGCTGTAG+AGG | + | chr8.3:32665522-32665541 | MS.gene074625:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 32665074 | 32665744 | 32665074 | ID=MS.gene074625 |
| chr8.3 | mRNA | 32665074 | 32665744 | 32665074 | ID=MS.gene074625.t1;Parent=MS.gene074625 |
| chr8.3 | exon | 32665074 | 32665184 | 32665074 | ID=MS.gene074625.t1.exon1;Parent=MS.gene074625.t1 |
| chr8.3 | CDS | 32665074 | 32665184 | 32665074 | ID=cds.MS.gene074625.t1;Parent=MS.gene074625.t1 |
| chr8.3 | exon | 32665285 | 32665561 | 32665285 | ID=MS.gene074625.t1.exon2;Parent=MS.gene074625.t1 |
| chr8.3 | CDS | 32665285 | 32665561 | 32665285 | ID=cds.MS.gene074625.t1;Parent=MS.gene074625.t1 |
| chr8.3 | exon | 32665656 | 32665744 | 32665656 | ID=MS.gene074625.t1.exon3;Parent=MS.gene074625.t1 |
| chr8.3 | CDS | 32665656 | 32665744 | 32665656 | ID=cds.MS.gene074625.t1;Parent=MS.gene074625.t1 |
| Gene Sequence |
| Protein sequence |
