Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056708.t1 | XP_003606906.2 | 99.6 | 488 | 2 | 0 | 1 | 488 | 1 | 488 | 1.60E-289 | 1004.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056708.t1 | Q9T034 | 80.7 | 488 | 90 | 3 | 1 | 487 | 1 | 485 | 2.1e-237 | 822.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056708.t1 | G7JG63 | 99.6 | 488 | 2 | 0 | 1 | 488 | 73 | 560 | 1.1e-289 | 1004.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056708 | MS.gene23259 | 0.909421 | 5.80E-82 | -1.69E-46 |
| MS.gene056708 | MS.gene23260 | 0.863034 | 3.32E-64 | -1.69E-46 |
| MS.gene056708 | MS.gene47520 | 0.807688 | 4.47E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056708.t1 | MTR_4g070040 | 99.590 | 488 | 2 | 0 | 1 | 488 | 73 | 560 | 0.0 | 1018 |
| MS.gene056708.t1 | MTR_7g038870 | 53.763 | 93 | 30 | 4 | 13 | 105 | 54 | 133 | 4.53e-18 | 80.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056708.t1 | AT4G39280 | 80.738 | 488 | 90 | 3 | 1 | 487 | 1 | 485 | 0.0 | 832 |
| MS.gene056708.t1 | AT4G39280 | 80.738 | 488 | 90 | 3 | 1 | 487 | 1 | 485 | 0.0 | 832 |
| MS.gene056708.t1 | AT3G58140 | 22.939 | 279 | 151 | 8 | 203 | 465 | 82 | 312 | 9.09e-12 | 67.4 |
Find 113 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTGAATTTCAACTAATTT+TGG | 0.096630 | 4.3:-35535074 | MS.gene056708:CDS |
| GCCAAAATTAGTTGAAATTC+AGG | 0.195550 | 4.3:+35535073 | None:intergenic |
| CCCATCGGCCGCAACATTTC+AGG | 0.247616 | 4.3:+35528366 | None:intergenic |
| CACAGGCTGCTAAGAATAAA+TGG | 0.253144 | 4.3:-35534157 | MS.gene056708:CDS |
| GAAGGGTTACTCGGTGAAAA+AGG | 0.272456 | 4.3:-35532771 | MS.gene056708:CDS |
| CAGTCTTCATCCACTGTTAA+AGG | 0.296408 | 4.3:-35532520 | MS.gene056708:intron |
| TAAGAATAAATGGGTGGAAA+TGG | 0.321478 | 4.3:-35534147 | MS.gene056708:CDS |
| GGCAGTTTCTTTGTTGTTGA+AGG | 0.347853 | 4.3:+35529918 | None:intergenic |
| ATCCCATACATTATCATTGT+TGG | 0.352476 | 4.3:+35528110 | None:intergenic |
| GCAATAACTTGGACATCTTC+AGG | 0.352913 | 4.3:+35528339 | None:intergenic |
| ACACAACTGCTGTTTCTTCC+CGG | 0.353252 | 4.3:-35529666 | MS.gene056708:CDS |
| GGAAACACTTATGCTACTTT+GGG | 0.368488 | 4.3:-35535100 | MS.gene056708:CDS |
| AGGAAACACTTATGCTACTT+TGG | 0.372317 | 4.3:-35535101 | MS.gene056708:CDS |
| TGACTCTATCTTCCACATCT+TGG | 0.374666 | 4.3:+35533893 | None:intergenic |
| AATGTCATCAAGAGTCTTCA+TGG | 0.375800 | 4.3:-35535281 | MS.gene056708:CDS |
| ATGGCAGAAGAAGCAGTTCT+TGG | 0.380478 | 4.3:-35535383 | MS.gene056708:CDS |
| TACGGTCCACTGCTTCATTT+CGG | 0.381127 | 4.3:+35529284 | None:intergenic |
| CTCACTCTCTGTGACTTAAT+AGG | 0.389562 | 4.3:-35528771 | MS.gene056708:CDS |
| AGGCCAACAATGATAATGTA+TGG | 0.396670 | 4.3:-35528113 | MS.gene056708:CDS |
| AAGAATAAATGGGTGGAAAT+GGG | 0.403506 | 4.3:-35534146 | MS.gene056708:CDS |
| TCTGCTTCTTCAAATACAAC+AGG | 0.409658 | 4.3:-35533866 | MS.gene056708:CDS |
| GTGAAGCAAATTCATGAATC+TGG | 0.409753 | 4.3:-35529876 | MS.gene056708:CDS |
| AACGATGAGATTAAAGATTC+AGG | 0.411017 | 4.3:-35535347 | MS.gene056708:CDS |
| CATGGGGACTTTCTCTTGAA+AGG | 0.415287 | 4.3:-35528317 | MS.gene056708:intron |
| TATCTCCAGAGATGAACTTC+AGG | 0.416797 | 4.3:-35535035 | MS.gene056708:intron |
| TTCATCTCTGGAGATACCTT+CGG | 0.418177 | 4.3:+35535042 | None:intergenic |
| GCTGAGTTCCATCAGATAGA+AGG | 0.424934 | 4.3:-35529253 | MS.gene056708:intron |
| TACTTTGTTGCAGTGGGGAG+TGG | 0.425940 | 4.3:-35532602 | MS.gene056708:intron |
| ATCACGAGCAGGATGTTGTT+GGG | 0.429335 | 4.3:+35530045 | None:intergenic |
| GTGCCAGCTGGTAAAGCATC+CGG | 0.436309 | 4.3:+35529647 | None:intergenic |
| ACCTGAAATGTTGCGGCCGA+TGG | 0.441480 | 4.3:-35528367 | MS.gene056708:CDS |
| GCTTTCAACATAATTATTTG+TGG | 0.443741 | 4.3:+35531712 | None:intergenic |
| TTCCCGGATGCTTTACCAGC+TGG | 0.449839 | 4.3:-35529650 | MS.gene056708:CDS |
| CATGATTTCTTCTCACGCTT+AGG | 0.453055 | 4.3:-35528741 | MS.gene056708:intron |
| CTTTCAACATAATTATTTGT+GGG | 0.453083 | 4.3:+35531713 | None:intergenic |
| AACTACCTGAAGTTCATCTC+TGG | 0.454281 | 4.3:+35535030 | None:intergenic |
| CCAAAATTAGTTGAAATTCA+GGG | 0.454614 | 4.3:+35535074 | None:intergenic |
| TCCATGCTAGGTTCTGTGTA+TGG | 0.455200 | 4.3:+35528545 | None:intergenic |
| CGTGGATATAGGATATTAAG+AGG | 0.457838 | 4.3:-35535147 | MS.gene056708:intron |
| TGGGAAAGCAACTCATATCT+AGG | 0.457897 | 4.3:-35534127 | MS.gene056708:CDS |
| TATCTAATGCTGCAGGTTTG+AGG | 0.459179 | 4.3:-35531741 | MS.gene056708:intron |
| GCTAAAGGTCAGCCTCTTGA+AGG | 0.483989 | 4.3:-35532546 | MS.gene056708:CDS |
| AAAGTCCCCATGCAATAACT+TGG | 0.488007 | 4.3:+35528328 | None:intergenic |
| ACCCGCTGAACATAATCTTC+AGG | 0.489399 | 4.3:+35529897 | None:intergenic |
| TCTGTGTATGGATTGTAAGC+AGG | 0.492584 | 4.3:+35528557 | None:intergenic |
| GAGTACAACTACACTGCTAA+AGG | 0.503823 | 4.3:-35532561 | MS.gene056708:CDS |
| CTCACGTACCTTCTATCTGA+TGG | 0.507401 | 4.3:+35529245 | None:intergenic |
| TATCTTCTTGCCTTTAACAG+TGG | 0.508276 | 4.3:+35532510 | None:intergenic |
| TCATCTCTGGAGATACCTTC+GGG | 0.514328 | 4.3:+35535043 | None:intergenic |
| TGGGTAGAAGTAGGAAACTC+TGG | 0.514681 | 4.3:-35528399 | MS.gene056708:CDS |
| CCTGAAATGTTGCGGCCGAT+GGG | 0.514857 | 4.3:-35528366 | MS.gene056708:CDS |
| ATCTGGTGGATATGAGTCTA+GGG | 0.518465 | 4.3:-35529859 | MS.gene056708:CDS |
| TTGGTAGATATGCATATGAC+TGG | 0.519858 | 4.3:-35529726 | MS.gene056708:intron |
| GAGCAGGATGTTGTTGGGGC+TGG | 0.519969 | 4.3:+35530050 | None:intergenic |
| GCCTGAAGATTATGTTCAGC+GGG | 0.522388 | 4.3:-35529898 | MS.gene056708:CDS |
| AATCTGGTGGATATGAGTCT+AGG | 0.523310 | 4.3:-35529860 | MS.gene056708:CDS |
| ACATGGGTGTTAACTGATGA+AGG | 0.528044 | 4.3:-35535121 | MS.gene056708:CDS |
| CAAATAATTATGTTGAAAGC+AGG | 0.529786 | 4.3:-35531709 | MS.gene056708:intron |
| TTTATAATCTAGGACATGGA+AGG | 0.529872 | 4.3:-35532789 | MS.gene056708:intron |
| AGCAGGTTTGAATTTCAGCT+TGG | 0.531031 | 4.3:+35528574 | None:intergenic |
| ATATTGCAAATGCAGGGCAT+TGG | 0.535921 | 4.3:-35532962 | MS.gene056708:intron |
| CAGTTGTGTGGGTCCGCAGA+AGG | 0.541428 | 4.3:+35529680 | None:intergenic |
| AATCACGAGCAGGATGTTGT+TGG | 0.550429 | 4.3:+35530044 | None:intergenic |
| TTATAATCTAGGACATGGAA+GGG | 0.551939 | 4.3:-35532788 | MS.gene056708:intron |
| AAGATGTCCAAGTTATTGCA+TGG | 0.554823 | 4.3:-35528335 | MS.gene056708:CDS |
| GATGCTTTACCAGCTGGCAC+AGG | 0.556280 | 4.3:-35529644 | MS.gene056708:intron |
| TCTTCAAATACAACAGGGAC+AGG | 0.557365 | 4.3:-35533860 | MS.gene056708:intron |
| AGGATATTAAGAGGGAGACA+TGG | 0.561811 | 4.3:-35535138 | MS.gene056708:CDS |
| ACAGGCTGCTAAGAATAAAT+GGG | 0.564845 | 4.3:-35534156 | MS.gene056708:CDS |
| CAAAATTAGTTGAAATTCAG+GGG | 0.565317 | 4.3:+35535075 | None:intergenic |
| CTTCAAGATAGCTTGTGCAC+AGG | 0.567618 | 4.3:-35534174 | MS.gene056708:CDS |
| ATGTTGTTGGGGCTGGAACA+AGG | 0.568157 | 4.3:+35530057 | None:intergenic |
| TGCCAGCTGGTAAAGCATCC+GGG | 0.570368 | 4.3:+35529648 | None:intergenic |
| GGCCAACAATGATAATGTAT+GGG | 0.574828 | 4.3:-35528112 | MS.gene056708:CDS |
| CTGGTTGAAGGTCCAAGATG+TGG | 0.575608 | 4.3:-35533905 | MS.gene056708:intron |
| CAGAGATCTCTTCGGCCACA+AGG | 0.576224 | 4.3:-35528078 | MS.gene056708:intron |
| GATAACATCAGAGATCTCTT+CGG | 0.577235 | 4.3:-35528086 | MS.gene056708:CDS |
| TGAGTCCTCGATCACATACA+AGG | 0.577373 | 4.3:+35528790 | None:intergenic |
| ACATACAAACCTGTGCCAGC+TGG | 0.581794 | 4.3:+35529635 | None:intergenic |
| AAGCAAATTCATGAATCTGG+TGG | 0.584637 | 4.3:-35529873 | MS.gene056708:CDS |
| GGGAAGAAACAGCAGTTGTG+TGG | 0.585320 | 4.3:+35529668 | None:intergenic |
| AGATGTCCAAGTTATTGCAT+GGG | 0.585697 | 4.3:-35528334 | MS.gene056708:CDS |
| GGATATTAAGAGGGAGACAT+GGG | 0.586886 | 4.3:-35535137 | MS.gene056708:CDS |
| TCCATACACAGAACCTAGCA+TGG | 0.588226 | 4.3:-35528546 | MS.gene056708:CDS |
| TAGGACATGGAAGGGTTACT+CGG | 0.591543 | 4.3:-35532780 | MS.gene056708:CDS |
| AAAGCAACTCATATCTAGGA+AGG | 0.600368 | 4.3:-35534123 | MS.gene056708:intron |
| GGAACTCAGCAAGATGTGTA+CGG | 0.600616 | 4.3:+35529266 | None:intergenic |
| CAAGCTATCTTGAAGACTGA+AGG | 0.601060 | 4.3:+35534182 | None:intergenic |
| TGAAGACTGCCACCTTCAAG+AGG | 0.603906 | 4.3:+35532534 | None:intergenic |
| TCGTATAACAGTTATCATGA+AGG | 0.610125 | 4.3:-35528432 | MS.gene056708:intron |
| CACCTGAAAATCTCCATGCT+AGG | 0.612290 | 4.3:+35528533 | None:intergenic |
| TAACTCGAGATAACTTTCAG+AGG | 0.616702 | 4.3:-35532706 | MS.gene056708:intron |
| TCAGGCCTTGTATGTGATCG+AGG | 0.620832 | 4.3:-35528795 | MS.gene056708:intron |
| TGTTCAGACCTGAAATGTTG+CGG | 0.621149 | 4.3:-35528374 | MS.gene056708:CDS |
| GGAAGAAACAGCAGTTGTGT+GGG | 0.622693 | 4.3:+35529669 | None:intergenic |
| GTGTCATGTGAATCACGAGC+AGG | 0.626400 | 4.3:+35530034 | None:intergenic |
| GGTGAATTTGCAGCTGAACG+TGG | 0.628430 | 4.3:-35535326 | MS.gene056708:CDS |
| AAAGGTCAGCCTCTTGAAGG+TGG | 0.635059 | 4.3:-35532543 | MS.gene056708:CDS |
| TGCATATGACTGGAAAAGAG+AGG | 0.636420 | 4.3:-35529716 | MS.gene056708:CDS |
| GGCTGCTAAGAATAAATGGG+TGG | 0.644736 | 4.3:-35534153 | MS.gene056708:CDS |
| ACATCTTCAGGAAGTCCCAT+CGG | 0.645307 | 4.3:+35528351 | None:intergenic |
| TCACGAGCAGGATGTTGTTG+GGG | 0.645590 | 4.3:+35530046 | None:intergenic |
| CTGCTTCTTCAAATACAACA+GGG | 0.647717 | 4.3:-35533865 | MS.gene056708:CDS |
| GATGTCCAAGTTATTGCATG+GGG | 0.652254 | 4.3:-35528333 | MS.gene056708:CDS |
| TGCCTGAAGATTATGTTCAG+CGG | 0.652900 | 4.3:-35529899 | MS.gene056708:CDS |
| GTGGATATAGGATATTAAGA+GGG | 0.659435 | 4.3:-35535146 | MS.gene056708:intron |
| CTGGTGGATATGAGTCTAGG+GGG | 0.660548 | 4.3:-35529857 | MS.gene056708:intron |
| TTTCAGATACGTCGATGCTG+TGG | 0.662586 | 4.3:-35535258 | MS.gene056708:intron |
| ATAAGACTCACCCCATACAG+TGG | 0.664162 | 4.3:+35531840 | None:intergenic |
| TGACATTGACGATTTCGTTG+TGG | 0.667924 | 4.3:+35535297 | None:intergenic |
| TGTATTCCGAAATGAAGCAG+TGG | 0.678250 | 4.3:-35529290 | MS.gene056708:CDS |
| TCTGGTGGATATGAGTCTAG+GGG | 0.691706 | 4.3:-35529858 | MS.gene056708:CDS |
| TCTCTGGAGATACCTTCGGG+CGG | 0.738824 | 4.3:+35535046 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 35527861 | 35535405 | 35527861 | ID=MS.gene056708 |
| chr4.3 | mRNA | 35527861 | 35535405 | 35527861 | ID=MS.gene056708.t1;Parent=MS.gene056708 |
| chr4.3 | exon | 35535259 | 35535405 | 35535259 | ID=MS.gene056708.t1.exon1;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35535259 | 35535405 | 35535259 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35535036 | 35535158 | 35535036 | ID=MS.gene056708.t1.exon2;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35535036 | 35535158 | 35535036 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35534124 | 35534216 | 35534124 | ID=MS.gene056708.t1.exon3;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35534124 | 35534216 | 35534124 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35533861 | 35533917 | 35533861 | ID=MS.gene056708.t1.exon4;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35533861 | 35533917 | 35533861 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35532914 | 35532969 | 35532914 | ID=MS.gene056708.t1.exon5;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35532914 | 35532969 | 35532914 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35532707 | 35532799 | 35532707 | ID=MS.gene056708.t1.exon6;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35532707 | 35532799 | 35532707 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35532521 | 35532611 | 35532521 | ID=MS.gene056708.t1.exon7;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35532521 | 35532611 | 35532521 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35531851 | 35531888 | 35531851 | ID=MS.gene056708.t1.exon8;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35531851 | 35531888 | 35531851 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35531710 | 35531748 | 35531710 | ID=MS.gene056708.t1.exon9;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35531710 | 35531748 | 35531710 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35530020 | 35530096 | 35530020 | ID=MS.gene056708.t1.exon10;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35530020 | 35530096 | 35530020 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35529858 | 35529942 | 35529858 | ID=MS.gene056708.t1.exon11;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35529858 | 35529942 | 35529858 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35529645 | 35529741 | 35529645 | ID=MS.gene056708.t1.exon12;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35529645 | 35529741 | 35529645 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35529254 | 35529350 | 35529254 | ID=MS.gene056708.t1.exon13;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35529254 | 35529350 | 35529254 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35528742 | 35528813 | 35528742 | ID=MS.gene056708.t1.exon14;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35528742 | 35528813 | 35528742 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35528536 | 35528602 | 35528536 | ID=MS.gene056708.t1.exon15;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35528536 | 35528602 | 35528536 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35528318 | 35528443 | 35528318 | ID=MS.gene056708.t1.exon16;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35528318 | 35528443 | 35528318 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35528079 | 35528133 | 35528079 | ID=MS.gene056708.t1.exon17;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35528079 | 35528133 | 35528079 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| chr4.3 | exon | 35527861 | 35527914 | 35527861 | ID=MS.gene056708.t1.exon18;Parent=MS.gene056708.t1 |
| chr4.3 | CDS | 35527861 | 35527914 | 35527861 | ID=cds.MS.gene056708.t1;Parent=MS.gene056708.t1 |
| Gene Sequence |
| Protein sequence |
