Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056867.t1 | XP_013468003.1 | 94.4 | 90 | 5 | 0 | 1 | 90 | 1 | 90 | 7.60E-44 | 186 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056867.t1 | Q9XH42 | 68.1 | 91 | 28 | 1 | 1 | 90 | 1 | 91 | 1.2e-34 | 146.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056867.t1 | A0A072VVB0 | 94.4 | 90 | 5 | 0 | 1 | 90 | 1 | 90 | 5.5e-44 | 186.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056867 | MS.gene81735 | 0.939551 | 1.05E-99 | -1.69E-46 |
| MS.gene056867 | MS.gene90152 | 0.804857 | 1.77E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056867.t1 | MTR_1g060190 | 94.444 | 90 | 5 | 0 | 1 | 90 | 1 | 90 | 3.05e-59 | 179 |
| MS.gene056867.t1 | MTR_2g086270 | 71.591 | 88 | 25 | 0 | 3 | 90 | 2 | 89 | 2.89e-45 | 143 |
| MS.gene056867.t1 | MTR_7g104460 | 63.333 | 90 | 33 | 0 | 1 | 90 | 1 | 90 | 5.22e-42 | 135 |
| MS.gene056867.t1 | MTR_0020s0120 | 57.143 | 84 | 36 | 0 | 7 | 90 | 6 | 89 | 2.14e-35 | 118 |
| MS.gene056867.t1 | MTR_0020s0120 | 57.143 | 84 | 36 | 0 | 7 | 90 | 6 | 89 | 2.60e-35 | 118 |
| MS.gene056867.t1 | MTR_7g084970 | 50.000 | 90 | 45 | 0 | 1 | 90 | 1 | 90 | 2.49e-30 | 105 |
| MS.gene056867.t1 | MTR_7g006690 | 48.276 | 87 | 44 | 1 | 5 | 90 | 5 | 91 | 1.97e-28 | 101 |
| MS.gene056867.t1 | MTR_6g033040 | 47.778 | 90 | 47 | 0 | 1 | 90 | 1 | 90 | 3.47e-28 | 100 |
| MS.gene056867.t1 | MTR_7g006630 | 50.588 | 85 | 41 | 1 | 7 | 90 | 2 | 86 | 2.35e-27 | 98.2 |
| MS.gene056867.t1 | MTR_7g085040 | 45.455 | 77 | 42 | 0 | 14 | 90 | 11 | 87 | 1.87e-24 | 90.9 |
| MS.gene056867.t1 | MTR_8g106840 | 43.956 | 91 | 50 | 1 | 1 | 91 | 1 | 90 | 3.45e-23 | 87.4 |
| MS.gene056867.t1 | MTR_7g085020 | 47.619 | 84 | 44 | 0 | 7 | 90 | 8 | 91 | 3.48e-23 | 87.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056867.t1 | AT5G03840 | 68.889 | 90 | 28 | 0 | 1 | 90 | 4 | 93 | 9.71e-45 | 142 |
| MS.gene056867.t1 | AT2G27550 | 63.736 | 91 | 32 | 1 | 1 | 90 | 1 | 91 | 1.61e-39 | 129 |
| MS.gene056867.t1 | AT5G62040 | 56.471 | 85 | 36 | 1 | 7 | 90 | 6 | 90 | 8.33e-33 | 112 |
| MS.gene056867.t1 | AT1G65480 | 54.762 | 84 | 38 | 0 | 7 | 90 | 7 | 90 | 6.30e-32 | 110 |
| MS.gene056867.t1 | AT1G65480 | 54.762 | 84 | 38 | 0 | 7 | 90 | 51 | 134 | 9.56e-32 | 110 |
| MS.gene056867.t1 | AT4G20370 | 52.381 | 84 | 40 | 0 | 7 | 90 | 7 | 90 | 3.52e-29 | 102 |
| MS.gene056867.t1 | AT1G18100 | 47.191 | 89 | 46 | 1 | 3 | 91 | 2 | 89 | 1.81e-24 | 90.9 |
Find 28 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAAATGTTCTTTCATATA+AGG | 0.264953 | 1.1:+45815559 | None:intergenic |
| ACTTCAGATCCACTAATTCT+TGG | 0.327033 | 1.1:-45816014 | MS.gene056867:CDS |
| ATGATAGACCCGGATGTTCC+TGG | 0.362611 | 1.1:-45815592 | MS.gene056867:CDS |
| CTAATTCTTGGGAGAGTGAT+AGG | 0.367036 | 1.1:-45816002 | MS.gene056867:CDS |
| ATCACTCTCCCAAGAATTAG+TGG | 0.393204 | 1.1:+45816005 | None:intergenic |
| CTTCAGATCCACTAATTCTT+GGG | 0.427576 | 1.1:-45816013 | MS.gene056867:CDS |
| TCACTTGGGCCAGGAACATC+CGG | 0.429086 | 1.1:+45815583 | None:intergenic |
| TCCAATCTCAATCCTTGGCT+TGG | 0.437325 | 1.1:+45815878 | None:intergenic |
| TCTCCTCCAATCTCAATCCT+TGG | 0.456612 | 1.1:+45815873 | None:intergenic |
| AATCTCAATCCTTGGCTTGG+TGG | 0.460499 | 1.1:+45815881 | None:intergenic |
| ACCAAGCCAAGGATTGAGAT+TGG | 0.488676 | 1.1:-45815879 | MS.gene056867:CDS |
| AACAACAAAGAAATCTTCAA+TGG | 0.490702 | 1.1:-45815930 | MS.gene056867:CDS |
| GGCTTGGTGGTAACTGAAGA+AGG | 0.508899 | 1.1:+45815894 | None:intergenic |
| ATATAAGGATCACTTGGGCC+AGG | 0.520355 | 1.1:+45815574 | None:intergenic |
| TCTTTCATATAAGGATCACT+TGG | 0.526849 | 1.1:+45815568 | None:intergenic |
| AATATAATACCAGTGTAAAG+AGG | 0.529031 | 1.1:+45815838 | None:intergenic |
| ATATGAAAGAACATTTGCAC+TGG | 0.561788 | 1.1:-45815555 | MS.gene056867:intron |
| ATATAATACCAGTGTAAAGA+GGG | 0.562460 | 1.1:+45815839 | None:intergenic |
| AAGGATTGAGATTGGAGGAG+AGG | 0.578470 | 1.1:-45815871 | MS.gene056867:CDS |
| CTTTCATATAAGGATCACTT+GGG | 0.579211 | 1.1:+45815569 | None:intergenic |
| GGTGGTAACTGAAGAAGGAA+AGG | 0.582057 | 1.1:+45815899 | None:intergenic |
| AAGCCAAGGATTGAGATTGG+AGG | 0.587134 | 1.1:-45815876 | MS.gene056867:CDS |
| GTGGTAACTGAAGAAGGAAA+GGG | 0.591441 | 1.1:+45815900 | None:intergenic |
| CTTCAGTTACCACCAAGCCA+AGG | 0.603242 | 1.1:-45815890 | MS.gene056867:CDS |
| CACTTGGGCCAGGAACATCC+GGG | 0.604987 | 1.1:+45815584 | None:intergenic |
| TCTGCAGGTCATGATAGACC+CGG | 0.613879 | 1.1:-45815602 | MS.gene056867:intron |
| AGATTGGAGGAGAGGACATG+AGG | 0.621266 | 1.1:-45815863 | MS.gene056867:CDS |
| CATGAGGTCCCTCTTTACAC+TGG | 0.660776 | 1.1:-45815847 | MS.gene056867:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAAAGTTATATTTTTCAA+TGG | - | chr1.1:45815809-45815828 | MS.gene056867:intron | 10.0% |
| !!! | ATAAATTCTTTTTTTTTTCA+TGG | - | chr1.1:45815651-45815670 | MS.gene056867:intron | 10.0% |
| !!! | GAAAAATATAACTTTTTTTT+AGG | + | chr1.1:45815807-45815826 | None:intergenic | 10.0% |
| !!! | TAAATTCTTTTTTTTTTCAT+GGG | - | chr1.1:45815652-45815671 | MS.gene056867:intron | 10.0% |
| !!! | TAATTATAGAAATGTATTTT+CGG | - | chr1.1:45815697-45815716 | MS.gene056867:intron | 10.0% |
| !!! | ATAAATTACAGTCATTTTTG+TGG | + | chr1.1:45815506-45815525 | None:intergenic | 20.0% |
| !!! | GTTATTATTTTGCTTGATTA+AGG | - | chr1.1:45816011-45816030 | MS.gene056867:CDS | 20.0% |
| ! | AACAACAAAGAAATCTTCAA+TGG | - | chr1.1:45815526-45815545 | MS.gene056867:intron | 25.0% |
| ! | AATATAATACCAGTGTAAAG+AGG | + | chr1.1:45815621-45815640 | None:intergenic | 25.0% |
| ! | ATATAATACCAGTGTAAAGA+GGG | + | chr1.1:45815620-45815639 | None:intergenic | 25.0% |
| ! | TAAAGCTATATATACCAACT+AGG | + | chr1.1:45815986-45816005 | None:intergenic | 25.0% |
| ! | TGCAAATGTTCTTTCATATA+AGG | + | chr1.1:45815900-45815919 | None:intergenic | 25.0% |
| !! | GAATTTAAGATAAACCTAGT+TGG | - | chr1.1:45815969-45815988 | MS.gene056867:CDS | 25.0% |
| ATATGAAAGAACATTTGCAC+TGG | - | chr1.1:45815901-45815920 | MS.gene056867:CDS | 30.0% | |
| CTTTCATATAAGGATCACTT+GGG | + | chr1.1:45815890-45815909 | None:intergenic | 30.0% | |
| TCTTTCATATAAGGATCACT+TGG | + | chr1.1:45815891-45815910 | None:intergenic | 30.0% | |
| !!! | ATTACAGTCATTTTTGTGGT+TGG | + | chr1.1:45815502-45815521 | None:intergenic | 30.0% |
| !!! | TTACAGTCATTTTTGTGGTT+GGG | + | chr1.1:45815501-45815520 | None:intergenic | 30.0% |
| ACTTCAGATCCACTAATTCT+TGG | - | chr1.1:45815442-45815461 | MS.gene056867:intron | 35.0% | |
| CTTCAGATCCACTAATTCTT+GGG | - | chr1.1:45815443-45815462 | MS.gene056867:intron | 35.0% | |
| !!! | TACAGTCATTTTTGTGGTTG+GGG | + | chr1.1:45815500-45815519 | None:intergenic | 35.0% |
| ATCACTCTCCCAAGAATTAG+TGG | + | chr1.1:45815454-45815473 | None:intergenic | 40.0% | |
| CTAATTCTTGGGAGAGTGAT+AGG | - | chr1.1:45815454-45815473 | MS.gene056867:intron | 40.0% | |
| GTGGTAACTGAAGAAGGAAA+GGG | + | chr1.1:45815559-45815578 | None:intergenic | 40.0% | |
| ! | GTTGTCTCTTCTTTTTCTGC+AGG | - | chr1.1:45815839-45815858 | MS.gene056867:intron | 40.0% |
| AAGCCAAGGATTGAGATTGG+AGG | - | chr1.1:45815580-45815599 | MS.gene056867:CDS | 45.0% | |
| AAGGATTGAGATTGGAGGAG+AGG | - | chr1.1:45815585-45815604 | MS.gene056867:CDS | 45.0% | |
| ACCAAGCCAAGGATTGAGAT+TGG | - | chr1.1:45815577-45815596 | MS.gene056867:CDS | 45.0% | |
| ATATAAGGATCACTTGGGCC+AGG | + | chr1.1:45815885-45815904 | None:intergenic | 45.0% | |
| GGTGGTAACTGAAGAAGGAA+AGG | + | chr1.1:45815560-45815579 | None:intergenic | 45.0% | |
| TCCAATCTCAATCCTTGGCT+TGG | + | chr1.1:45815581-45815600 | None:intergenic | 45.0% | |
| TCTCCTCCAATCTCAATCCT+TGG | + | chr1.1:45815586-45815605 | None:intergenic | 45.0% | |
| !! | AATCTCAATCCTTGGCTTGG+TGG | + | chr1.1:45815578-45815597 | None:intergenic | 45.0% |
| !!! | AAAAATATAACTTTTTTTTA+GGG | + | chr1.1:45815806-45815825 | None:intergenic | 5.0% |
| AGATTGGAGGAGAGGACATG+AGG | - | chr1.1:45815593-45815612 | MS.gene056867:CDS | 50.0% | |
| ATGATAGACCCGGATGTTCC+TGG | - | chr1.1:45815864-45815883 | MS.gene056867:CDS | 50.0% | |
| CATGAGGTCCCTCTTTACAC+TGG | - | chr1.1:45815609-45815628 | MS.gene056867:intron | 50.0% | |
| CTTCAGTTACCACCAAGCCA+AGG | - | chr1.1:45815566-45815585 | MS.gene056867:CDS | 50.0% | |
| TCTGCAGGTCATGATAGACC+CGG | - | chr1.1:45815854-45815873 | MS.gene056867:CDS | 50.0% | |
| !! | GGCTTGGTGGTAACTGAAGA+AGG | + | chr1.1:45815565-45815584 | None:intergenic | 50.0% |
| TCACTTGGGCCAGGAACATC+CGG | + | chr1.1:45815876-45815895 | None:intergenic | 55.0% | |
| CACTTGGGCCAGGAACATCC+GGG | + | chr1.1:45815875-45815894 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 45815430 | 45816048 | 45815430 | ID=MS.gene056867 |
| chr1.1 | mRNA | 45815430 | 45816048 | 45815430 | ID=MS.gene056867.t1;Parent=MS.gene056867 |
| chr1.1 | exon | 45815848 | 45816048 | 45815848 | ID=MS.gene056867.t1.exon1;Parent=MS.gene056867.t1 |
| chr1.1 | CDS | 45815848 | 45816048 | 45815848 | ID=cds.MS.gene056867.t1;Parent=MS.gene056867.t1 |
| chr1.1 | exon | 45815556 | 45815617 | 45815556 | ID=MS.gene056867.t1.exon2;Parent=MS.gene056867.t1 |
| chr1.1 | CDS | 45815556 | 45815617 | 45815556 | ID=cds.MS.gene056867.t1;Parent=MS.gene056867.t1 |
| chr1.1 | exon | 45815430 | 45815445 | 45815430 | ID=MS.gene056867.t1.exon3;Parent=MS.gene056867.t1 |
| chr1.1 | CDS | 45815430 | 45815445 | 45815430 | ID=cds.MS.gene056867.t1;Parent=MS.gene056867.t1 |
| Gene Sequence |
| Protein sequence |
