Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81735.t1 | XP_013468003.1 | 91.4 | 93 | 8 | 0 | 1 | 93 | 1 | 93 | 9.40E-42 | 179.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81735.t1 | Q9XH42 | 69.3 | 88 | 26 | 1 | 1 | 87 | 1 | 88 | 2.0e-32 | 139.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81735.t1 | A0A072VVB0 | 91.4 | 93 | 8 | 0 | 1 | 93 | 1 | 93 | 6.8e-42 | 179.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051199 | MS.gene81735 | 0.810486 | 1.13E-50 | -1.69E-46 |
| MS.gene052456 | MS.gene81735 | 0.838168 | 3.37E-57 | -1.69E-46 |
| MS.gene053383 | MS.gene81735 | 0.813408 | 2.60E-51 | -1.69E-46 |
| MS.gene055359 | MS.gene81735 | 0.802316 | 5.94E-49 | -1.69E-46 |
| MS.gene056867 | MS.gene81735 | 0.939551 | 1.05E-99 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81735.t1 | MTR_1g060190 | 91.398 | 93 | 8 | 0 | 1 | 93 | 1 | 93 | 5.93e-58 | 177 |
| MS.gene81735.t1 | MTR_2g086270 | 69.231 | 91 | 28 | 0 | 3 | 93 | 2 | 92 | 4.23e-43 | 139 |
| MS.gene81735.t1 | MTR_7g104460 | 61.290 | 93 | 36 | 0 | 1 | 93 | 1 | 93 | 1.07e-39 | 130 |
| MS.gene81735.t1 | MTR_0020s0120 | 55.172 | 87 | 39 | 0 | 7 | 93 | 6 | 92 | 4.22e-33 | 114 |
| MS.gene81735.t1 | MTR_0020s0120 | 55.172 | 87 | 39 | 0 | 7 | 93 | 6 | 92 | 5.17e-33 | 114 |
| MS.gene81735.t1 | MTR_7g084970 | 47.312 | 93 | 49 | 0 | 1 | 93 | 1 | 93 | 2.42e-27 | 99.4 |
| MS.gene81735.t1 | MTR_7g006690 | 46.667 | 90 | 47 | 1 | 5 | 93 | 5 | 94 | 1.69e-26 | 97.4 |
| MS.gene81735.t1 | MTR_6g033040 | 45.161 | 93 | 51 | 0 | 1 | 93 | 1 | 93 | 2.12e-25 | 94.4 |
| MS.gene81735.t1 | MTR_7g006630 | 48.864 | 88 | 44 | 1 | 7 | 93 | 2 | 89 | 3.25e-25 | 94.0 |
| MS.gene81735.t1 | MTR_7g085040 | 43.678 | 87 | 46 | 1 | 7 | 93 | 7 | 90 | 4.91e-22 | 85.9 |
| MS.gene81735.t1 | MTR_7g085020 | 45.977 | 87 | 47 | 0 | 7 | 93 | 8 | 94 | 2.28e-21 | 84.3 |
| MS.gene81735.t1 | MTR_8g106840 | 43.011 | 93 | 52 | 1 | 1 | 93 | 1 | 92 | 2.78e-20 | 81.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81735.t1 | AT5G03840 | 66.667 | 93 | 31 | 0 | 1 | 93 | 4 | 96 | 1.62e-42 | 138 |
| MS.gene81735.t1 | AT2G27550 | 61.702 | 94 | 35 | 1 | 1 | 93 | 1 | 94 | 6.83e-37 | 124 |
| MS.gene81735.t1 | AT5G62040 | 54.545 | 88 | 39 | 1 | 7 | 93 | 6 | 93 | 1.32e-30 | 107 |
| MS.gene81735.t1 | AT1G65480 | 51.724 | 87 | 42 | 0 | 7 | 93 | 7 | 93 | 4.17e-29 | 103 |
| MS.gene81735.t1 | AT1G65480 | 51.724 | 87 | 42 | 0 | 7 | 93 | 51 | 137 | 9.10e-29 | 104 |
| MS.gene81735.t1 | AT4G20370 | 49.425 | 87 | 44 | 0 | 7 | 93 | 7 | 93 | 1.46e-26 | 97.4 |
| MS.gene81735.t1 | AT1G18100 | 46.154 | 91 | 48 | 1 | 3 | 93 | 2 | 91 | 1.06e-21 | 85.1 |
Find 31 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAAATGTTCTTTCATATA+AGG | 0.264953 | 1.3:+43620871 | None:intergenic |
| ACTTCAGATCCACTAATTCT+TGG | 0.327033 | 1.3:-43621326 | MS.gene81735:CDS |
| ATGATAGACCCGGATGTTCC+TGG | 0.362611 | 1.3:-43620904 | MS.gene81735:CDS |
| GAAAGCTATGCAGTAAATTC+TGG | 0.366658 | 1.3:+43620243 | None:intergenic |
| CTAATTCTTGGGAGAGTGAT+AGG | 0.367036 | 1.3:-43621314 | MS.gene81735:CDS |
| ATCACTCTCCCAAGAATTAG+TGG | 0.393204 | 1.3:+43621317 | None:intergenic |
| TACTGCATAGCTTTCAATCT+CGG | 0.414727 | 1.3:-43620235 | MS.gene81735:CDS |
| CTTCAGATCCACTAATTCTT+GGG | 0.427576 | 1.3:-43621325 | MS.gene81735:CDS |
| TCACTTGGGCCAGGAACATC+CGG | 0.429086 | 1.3:+43620895 | None:intergenic |
| TCCAATCTCAATCCTTGGCT+TGG | 0.437325 | 1.3:+43621190 | None:intergenic |
| TCTCCTCCAATCTCAATCCT+TGG | 0.456612 | 1.3:+43621185 | None:intergenic |
| AATCTCAATCCTTGGCTTGG+TGG | 0.460499 | 1.3:+43621193 | None:intergenic |
| ACCAAGCCAAGGATTGAGAT+TGG | 0.488676 | 1.3:-43621191 | MS.gene81735:CDS |
| AACAACAAAGAAATCTTCAA+TGG | 0.490702 | 1.3:-43621242 | MS.gene81735:CDS |
| GGCTTGGTGGTAACTGAAGA+AGG | 0.508899 | 1.3:+43621206 | None:intergenic |
| ATATAAGGATCACTTGGGCC+AGG | 0.520355 | 1.3:+43620886 | None:intergenic |
| TCTTTCATATAAGGATCACT+TGG | 0.526849 | 1.3:+43620880 | None:intergenic |
| AATATAATACCAGTGTAAAG+AGG | 0.529031 | 1.3:+43621150 | None:intergenic |
| ATATGAAAGAACATTTGCAC+TGG | 0.561788 | 1.3:-43620867 | MS.gene81735:intron |
| ATATAATACCAGTGTAAAGA+GGG | 0.562460 | 1.3:+43621151 | None:intergenic |
| AAGGATTGAGATTGGAGGAG+AGG | 0.578470 | 1.3:-43621183 | MS.gene81735:CDS |
| CTTTCATATAAGGATCACTT+GGG | 0.579211 | 1.3:+43620881 | None:intergenic |
| GGTGGTAACTGAAGAAGGAA+AGG | 0.582057 | 1.3:+43621211 | None:intergenic |
| AAGCCAAGGATTGAGATTGG+AGG | 0.587134 | 1.3:-43621188 | MS.gene81735:CDS |
| GTGGTAACTGAAGAAGGAAA+GGG | 0.591441 | 1.3:+43621212 | None:intergenic |
| CTTCAGTTACCACCAAGCCA+AGG | 0.603242 | 1.3:-43621202 | MS.gene81735:CDS |
| CACTTGGGCCAGGAACATCC+GGG | 0.604987 | 1.3:+43620896 | None:intergenic |
| TCTGCAGGTCATGATAGACC+CGG | 0.613879 | 1.3:-43620914 | MS.gene81735:intron |
| AGATTGGAGGAGAGGACATG+AGG | 0.621266 | 1.3:-43621175 | MS.gene81735:CDS |
| CGGCGTTCTATAAGATCGAA+CGG | 0.652002 | 1.3:-43620215 | MS.gene81735:CDS |
| CATGAGGTCCCTCTTTACAC+TGG | 0.660776 | 1.3:-43621159 | MS.gene81735:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAAAGTTATATTTTTCAA+TGG | - | chr1.3:43620590-43620609 | MS.gene81735:intron | 10.0% |
| !!! | AAATTCTTTTTTTTTTTCAT+GGG | - | chr1.3:43620434-43620453 | MS.gene81735:intron | 10.0% |
| !!! | GAAAAATATAACTTTTTTTT+AGG | + | chr1.3:43620588-43620607 | None:intergenic | 10.0% |
| !!! | TAAATTCTTTTTTTTTTTCA+TGG | - | chr1.3:43620433-43620452 | MS.gene81735:intron | 10.0% |
| !!! | TAATTATAGAAATGTATTTT+CGG | - | chr1.3:43620478-43620497 | MS.gene81735:intron | 10.0% |
| !! | AAACAAATTAAAAGTGATTA+TGG | - | chr1.3:43620830-43620849 | MS.gene81735:intron | 15.0% |
| !! | AAACACATAAATAAAATAGT+TGG | + | chr1.3:43620945-43620964 | None:intergenic | 15.0% |
| !!! | ATAAATTACAGTCATTTTTG+TGG | + | chr1.3:43620287-43620306 | None:intergenic | 20.0% |
| !!! | GTTATTATTTTGCTTGATTA+AGG | - | chr1.3:43620792-43620811 | MS.gene81735:intron | 20.0% |
| !!! | TCAAGTTTATTTTCTTTATG+TGG | + | chr1.3:43621261-43621280 | None:intergenic | 20.0% |
| ! | AACAACAAAGAAATCTTCAA+TGG | - | chr1.3:43620307-43620326 | MS.gene81735:intron | 25.0% |
| ! | AATATAATACCAGTGTAAAG+AGG | + | chr1.3:43620402-43620421 | None:intergenic | 25.0% |
| ! | AATGAGGGTTATGTAATAAA+TGG | + | chr1.3:43621028-43621047 | None:intergenic | 25.0% |
| ! | ATATAATACCAGTGTAAAGA+GGG | + | chr1.3:43620401-43620420 | None:intergenic | 25.0% |
| ! | TAAAGCTATATATACCAACT+AGG | + | chr1.3:43620767-43620786 | None:intergenic | 25.0% |
| ! | TGCAAATGTTCTTTCATATA+AGG | + | chr1.3:43620681-43620700 | None:intergenic | 25.0% |
| !! | GAATTTAAGATAAACCTAGT+TGG | - | chr1.3:43620750-43620769 | MS.gene81735:intron | 25.0% |
| !! | TATGATTTGTACGAAAATGA+GGG | + | chr1.3:43621043-43621062 | None:intergenic | 25.0% |
| !!! | ATACTACTTGCTTTTCTAAA+AGG | + | chr1.3:43621166-43621185 | None:intergenic | 25.0% |
| !!! | TACTACTTGCTTTTCTAAAA+GGG | + | chr1.3:43621165-43621184 | None:intergenic | 25.0% |
| !!! | TTAGTCTTAGATATTACTTG+TGG | + | chr1.3:43620868-43620887 | None:intergenic | 25.0% |
| ATATGAAAGAACATTTGCAC+TGG | - | chr1.3:43620682-43620701 | MS.gene81735:intron | 30.0% | |
| ATTAAGTTAGACTCCGTAAT+TGG | - | chr1.3:43620909-43620928 | MS.gene81735:CDS | 30.0% | |
| CTTTCATATAAGGATCACTT+GGG | + | chr1.3:43620671-43620690 | None:intergenic | 30.0% | |
| GGGTTATGTAATAAATGGTA+CGG | + | chr1.3:43621023-43621042 | None:intergenic | 30.0% | |
| TCTTTCATATAAGGATCACT+TGG | + | chr1.3:43620672-43620691 | None:intergenic | 30.0% | |
| TTAAGTTAGACTCCGTAATT+GGG | - | chr1.3:43620910-43620929 | MS.gene81735:CDS | 30.0% | |
| ! | GTATGATTTGTACGAAAATG+AGG | + | chr1.3:43621044-43621063 | None:intergenic | 30.0% |
| !! | GGGTCAATCAAATTGTTTTA+TGG | + | chr1.3:43621223-43621242 | None:intergenic | 30.0% |
| !!! | ATTACAGTCATTTTTGTGGT+TGG | + | chr1.3:43620283-43620302 | None:intergenic | 30.0% |
| !!! | TTACAGTCATTTTTGTGGTT+GGG | + | chr1.3:43620282-43620301 | None:intergenic | 30.0% |
| ACTTCAGATCCACTAATTCT+TGG | - | chr1.3:43620223-43620242 | MS.gene81735:CDS | 35.0% | |
| CTTCAGATCCACTAATTCTT+GGG | - | chr1.3:43620224-43620243 | MS.gene81735:CDS | 35.0% | |
| GAAAGCTATGCAGTAAATTC+TGG | + | chr1.3:43621309-43621328 | None:intergenic | 35.0% | |
| TACTGCATAGCTTTCAATCT+CGG | - | chr1.3:43621314-43621333 | MS.gene81735:CDS | 35.0% | |
| !!! | TACAGTCATTTTTGTGGTTG+GGG | + | chr1.3:43620281-43620300 | None:intergenic | 35.0% |
| ATCACTCTCCCAAGAATTAG+TGG | + | chr1.3:43620235-43620254 | None:intergenic | 40.0% | |
| CTAATTCTTGGGAGAGTGAT+AGG | - | chr1.3:43620235-43620254 | MS.gene81735:CDS | 40.0% | |
| GACCAGATCGATCAATTTAG+AGG | + | chr1.3:43621083-43621102 | None:intergenic | 40.0% | |
| GTGGTAACTGAAGAAGGAAA+GGG | + | chr1.3:43620340-43620359 | None:intergenic | 40.0% | |
| TGCCTCTAAATTGATCGATC+TGG | - | chr1.3:43621078-43621097 | MS.gene81735:intron | 40.0% | |
| TGGTGTTCGATTCCCAATTA+CGG | + | chr1.3:43620925-43620944 | None:intergenic | 40.0% | |
| ! | GTTGTCTCTTCTTTTTCTGC+AGG | - | chr1.3:43620620-43620639 | MS.gene81735:intron | 40.0% |
| ! | TAGAGGCATATGACTGCTAA+GGG | + | chr1.3:43621066-43621085 | None:intergenic | 40.0% |
| ! | TTAGAGGCATATGACTGCTA+AGG | + | chr1.3:43621067-43621086 | None:intergenic | 40.0% |
| AAGCCAAGGATTGAGATTGG+AGG | - | chr1.3:43620361-43620380 | MS.gene81735:intron | 45.0% | |
| AAGGATTGAGATTGGAGGAG+AGG | - | chr1.3:43620366-43620385 | MS.gene81735:intron | 45.0% | |
| ACCAAGCCAAGGATTGAGAT+TGG | - | chr1.3:43620358-43620377 | MS.gene81735:intron | 45.0% | |
| ATATAAGGATCACTTGGGCC+AGG | + | chr1.3:43620666-43620685 | None:intergenic | 45.0% | |
| ATGTGGCTACACAAGACGTA+CGG | + | chr1.3:43621244-43621263 | None:intergenic | 45.0% | |
| CGGCGTTCTATAAGATCGAA+CGG | - | chr1.3:43621334-43621353 | MS.gene81735:CDS | 45.0% | |
| GGTGGTAACTGAAGAAGGAA+AGG | + | chr1.3:43620341-43620360 | None:intergenic | 45.0% | |
| TCCAATCTCAATCCTTGGCT+TGG | + | chr1.3:43620362-43620381 | None:intergenic | 45.0% | |
| TCTCCTCCAATCTCAATCCT+TGG | + | chr1.3:43620367-43620386 | None:intergenic | 45.0% | |
| !! | AATCTCAATCCTTGGCTTGG+TGG | + | chr1.3:43620359-43620378 | None:intergenic | 45.0% |
| !!! | AAAAATATAACTTTTTTTTA+GGG | + | chr1.3:43620587-43620606 | None:intergenic | 5.0% |
| AGATTGGAGGAGAGGACATG+AGG | - | chr1.3:43620374-43620393 | MS.gene81735:intron | 50.0% | |
| ATGATAGACCCGGATGTTCC+TGG | - | chr1.3:43620645-43620664 | MS.gene81735:intron | 50.0% | |
| CATGAGGTCCCTCTTTACAC+TGG | - | chr1.3:43620390-43620409 | MS.gene81735:intron | 50.0% | |
| CTTCAGTTACCACCAAGCCA+AGG | - | chr1.3:43620347-43620366 | MS.gene81735:intron | 50.0% | |
| TCTGCAGGTCATGATAGACC+CGG | - | chr1.3:43620635-43620654 | MS.gene81735:intron | 50.0% | |
| TGTGGCTACACAAGACGTAC+GGG | + | chr1.3:43621243-43621262 | None:intergenic | 50.0% | |
| !! | GGCTTGGTGGTAACTGAAGA+AGG | + | chr1.3:43620346-43620365 | None:intergenic | 50.0% |
| TCACTTGGGCCAGGAACATC+CGG | + | chr1.3:43620657-43620676 | None:intergenic | 55.0% | |
| CACTTGGGCCAGGAACATCC+GGG | + | chr1.3:43620656-43620675 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 43620211 | 43621360 | 43620211 | ID=MS.gene81735 |
| chr1.3 | mRNA | 43620211 | 43621360 | 43620211 | ID=MS.gene81735.t1;Parent=MS.gene81735 |
| chr1.3 | exon | 43621160 | 43621360 | 43621160 | ID=MS.gene81735.t1.exon1;Parent=MS.gene81735.t1 |
| chr1.3 | CDS | 43621160 | 43621360 | 43621160 | ID=cds.MS.gene81735.t1;Parent=MS.gene81735.t1 |
| chr1.3 | exon | 43620868 | 43620929 | 43620868 | ID=MS.gene81735.t1.exon2;Parent=MS.gene81735.t1 |
| chr1.3 | CDS | 43620868 | 43620929 | 43620868 | ID=cds.MS.gene81735.t1;Parent=MS.gene81735.t1 |
| chr1.3 | exon | 43620211 | 43620313 | 43620211 | ID=MS.gene81735.t1.exon3;Parent=MS.gene81735.t1 |
| chr1.3 | CDS | 43620211 | 43620313 | 43620211 | ID=cds.MS.gene81735.t1;Parent=MS.gene81735.t1 |
| Gene Sequence |
| Protein sequence |
