AlfalfaGEDB | MS.gene90152

M. sativa cultivar XinJiangDaYe / MS.gene90152

KEGG: K24345
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13833.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90152.t1	XP_003596520.1	90.4	114	11	0	1	114	1	114	3.50E-54	220.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90152.t1	O81831	60.4	96	36	2	8	101	18	113	1.0e-25	117.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90152.t1	Q2HVR3	90.4	114	11	0	1	114	1	114	2.5e-54	220.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050563	MS.gene90152	0.80646	8.14E-50	-1.69E-46
MS.gene051199	MS.gene90152	0.823172	1.60E-53	-1.69E-46
MS.gene053383	MS.gene90152	0.865464	5.80E-65	-1.69E-46
MS.gene055359	MS.gene90152	0.822195	2.70E-53	-1.69E-46
MS.gene056867	MS.gene90152	0.804857	1.77E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90152.t1	MTR_2g081350	92.982	114	8	0	1	114	1	114	3.46e-72	210
MS.gene90152.t1	MTR_2g081440	90.351	114	11	0	1	114	1	114	9.10e-71	207
MS.gene90152.t1	MTR_2g081300	89.815	108	11	0	7	114	3	110	1.57e-67	198
MS.gene90152.t1	MTR_2g081580	64.286	98	34	1	6	102	7	104	3.47e-38	125
MS.gene90152.t1	MTR_8g466250	66.667	96	31	1	9	103	11	106	8.13e-38	124
MS.gene90152.t1	MTR_2g011400	65.957	94	31	1	9	101	14	107	2.30e-37	122
MS.gene90152.t1	MTR_2g089210	59.140	93	37	1	7	98	8	100	1.45e-33	113
MS.gene90152.t1	MTR_1g052885	61.538	91	34	1	9	98	6	96	2.22e-32	110
MS.gene90152.t1	MTR_8g027180	54.348	92	41	1	8	98	18	109	1.07e-27	98.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90152.t1	AT5G54490	58.947	95	37	2	9	101	19	113	9.69e-34	114
MS.gene90152.t1	AT4G27280	60.417	96	36	2	8	101	18	113	3.48e-33	112
MS.gene90152.t1	AT2G46600	52.747	91	42	1	9	98	22	112	1.38e-26	96.3

Find 27 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAACGGGTTTCATTTGCTTA+TGG	0.242149	2.3:+17031902	MS.gene90152:CDS
CCATCCATGGTTGCGAGTTT+AGG	0.278334	2.3:+17031852	MS.gene90152:CDS
CATGCTAATGGAAGGTGATT+TGG	0.335601	2.3:+17032013	MS.gene90152:CDS
TTGTTTCATGCTTGGCTTGG+AGG	0.430866	2.3:+17031968	MS.gene90152:CDS
TAGGTTGAGTCCATGTTTAA+TGG	0.441618	2.3:+17032082	MS.gene90152:CDS
AGATGGTGCTTTAAATCAAA+TGG	0.452147	2.3:+17032040	MS.gene90152:CDS
AGAGCTCATGTACATGCTAA+TGG	0.454187	2.3:+17032001	MS.gene90152:CDS
AAGATGAATTGTTTCATGCT+TGG	0.467632	2.3:+17031960	MS.gene90152:CDS
CCTAAACTCGCAACCATGGA+TGG	0.475656	2.3:-17031852	None:intergenic
CATGCTTGGCTTGGAGGTGA+GGG	0.488966	2.3:+17031974	MS.gene90152:CDS
TGAGGACTACTTTCCATCCA+TGG	0.498192	2.3:+17031839	MS.gene90152:CDS
GAATTGTTTCATGCTTGGCT+TGG	0.505364	2.3:+17031965	MS.gene90152:CDS
TTATAGGTGAACTTTGCAAC+GGG	0.523665	2.3:+17031886	MS.gene90152:CDS
TTGAGTCCATGTTTAATGGA+TGG	0.531601	2.3:+17032086	MS.gene90152:CDS
TCATGCTTGGCTTGGAGGTG+AGG	0.539439	2.3:+17031973	MS.gene90152:CDS
AACATTGGATTCACACCTTG+AGG	0.552010	2.3:-17032128	None:intergenic
TTTGCTTATGGATGTCAACA+AGG	0.557910	2.3:+17031914	MS.gene90152:CDS
TGCTCCTAAACTCGCAACCA+TGG	0.581169	2.3:-17031856	None:intergenic
GTTGCGAGTTTAGGAGCAGA+AGG	0.581774	2.3:+17031861	MS.gene90152:CDS
TTTATAGGTGAACTTTGCAA+CGG	0.586350	2.3:+17031885	MS.gene90152:CDS
GAGTGTCATCACATTAACAT+TGG	0.590328	2.3:-17032143	None:intergenic
TTAGGTCCATCCATTAAACA+TGG	0.608146	2.3:-17032092	None:intergenic
CTCATGTACATGCTAATGGA+AGG	0.614676	2.3:+17032005	MS.gene90152:CDS
GAAGGTGATTTGGATGGAGA+TGG	0.627275	2.3:+17032023	MS.gene90152:CDS
AAAATGTGCACAAATCCTCA+AGG	0.645113	2.3:+17032113	MS.gene90152:CDS
TTGCTTATGGATGTCAACAA+GGG	0.661501	2.3:+17031915	MS.gene90152:CDS
CTAATGGAAGGTGATTTGGA+TGG	0.674005	2.3:+17032017	MS.gene90152:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGATTTTTACATGGATTTTG+AGG	+	chr2.3:17031821-17031840	MS.gene90152:CDS	25.0%
	AAGATGAATTGTTTCATGCT+TGG	+	chr2.3:17031960-17031979	MS.gene90152:CDS	30.0%
	TTTATAGGTGAACTTTGCAA+CGG	+	chr2.3:17031885-17031904	MS.gene90152:CDS	30.0%
!	AGATGGTGCTTTAAATCAAA+TGG	+	chr2.3:17032040-17032059	MS.gene90152:CDS	30.0%
!	AGTTTTGCATTCTCATGTTT+AGG	+	chr2.3:17032063-17032082	MS.gene90152:CDS	30.0%
	AAAATGTGCACAAATCCTCA+AGG	+	chr2.3:17032113-17032132	MS.gene90152:CDS	35.0%
	TTAGGTCCATCCATTAAACA+TGG	-	chr2.3:17032095-17032114	None:intergenic	35.0%
	TTATAGGTGAACTTTGCAAC+GGG	+	chr2.3:17031886-17031905	MS.gene90152:CDS	35.0%
	TTGAGTCCATGTTTAATGGA+TGG	+	chr2.3:17032086-17032105	MS.gene90152:CDS	35.0%
	TTGCTTATGGATGTCAACAA+GGG	+	chr2.3:17031915-17031934	MS.gene90152:CDS	35.0%
	TTTGCTTATGGATGTCAACA+AGG	+	chr2.3:17031914-17031933	MS.gene90152:CDS	35.0%
!	TAGGTTGAGTCCATGTTTAA+TGG	+	chr2.3:17032082-17032101	MS.gene90152:CDS	35.0%
!	TGAGGATTTGTGCACATTTT+AGG	-	chr2.3:17032113-17032132	None:intergenic	35.0%
!!	TTAGGAGCAGAAGGTTTTAT+AGG	+	chr2.3:17031870-17031889	MS.gene90152:CDS	35.0%
	AACATTGGATTCACACCTTG+AGG	-	chr2.3:17032131-17032150	None:intergenic	40.0%
	AGAGCTCATGTACATGCTAA+TGG	+	chr2.3:17032001-17032020	MS.gene90152:CDS	40.0%
	CATGCTAATGGAAGGTGATT+TGG	+	chr2.3:17032013-17032032	MS.gene90152:CDS	40.0%
	CTCATGTACATGCTAATGGA+AGG	+	chr2.3:17032005-17032024	MS.gene90152:CDS	40.0%
	GAATTGTTTCATGCTTGGCT+TGG	+	chr2.3:17031965-17031984	MS.gene90152:CDS	40.0%
!	CAACGGGTTTCATTTGCTTA+TGG	+	chr2.3:17031902-17031921	MS.gene90152:CDS	40.0%
!	CTAATGGAAGGTGATTTGGA+TGG	+	chr2.3:17032017-17032036	MS.gene90152:CDS	40.0%
	TGAGGACTACTTTCCATCCA+TGG	+	chr2.3:17031839-17031858	MS.gene90152:CDS	45.0%
!	GAAGGTGATTTGGATGGAGA+TGG	+	chr2.3:17032023-17032042	MS.gene90152:CDS	45.0%
!!	TTGTTTCATGCTTGGCTTGG+AGG	+	chr2.3:17031968-17031987	MS.gene90152:CDS	45.0%
	CCATCCATGGTTGCGAGTTT+AGG	+	chr2.3:17031852-17031871	MS.gene90152:CDS	50.0%
	CCTAAACTCGCAACCATGGA+TGG	-	chr2.3:17031855-17031874	None:intergenic	50.0%
	GTTGCGAGTTTAGGAGCAGA+AGG	+	chr2.3:17031861-17031880	MS.gene90152:CDS	50.0%
	TGCTCCTAAACTCGCAACCA+TGG	-	chr2.3:17031859-17031878	None:intergenic	50.0%
!!	CATGCTTGGCTTGGAGGTGA+GGG	+	chr2.3:17031974-17031993	MS.gene90152:CDS	55.0%
!!	TCATGCTTGGCTTGGAGGTG+AGG	+	chr2.3:17031973-17031992	MS.gene90152:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	17031813	17032157	17031813	ID=MS.gene90152
chr2.3	mRNA	17031813	17032157	17031813	ID=MS.gene90152.t1;Parent=MS.gene90152
chr2.3	exon	17031813	17032157	17031813	ID=MS.gene90152.t1.exon1;Parent=MS.gene90152.t1
chr2.3	CDS	17031813	17032157	17031813	ID=cds.MS.gene90152.t1;Parent=MS.gene90152.t1

Gene Sequence	>MS.gene90152 ATGGATTTAGATTTTTACATGGATTTTGAGGACTACTTTCCATCCATGGTTGCGAGTTTAGGAGCAGAAGGTTTTATAGGTGAACTTTGCAACGGGTTTCATTTGCTTATGGATGTCAACAAGGGTCTTATAACATTTGAGAGTTTGAAGATGAATTGTTTCATGCTTGGCTTGGAGGTGAGGGACGAAGAGCTCATGTACATGCTAATGGAAGGTGATTTGGATGGAGATGGTGCTTTAAATCAAATGGAGTTTTGCATTCTCATGTTTAGGTTGAGTCCATGTTTAATGGATGGACCTAAAATGTGCACAAATCCTCAAGGTGTGAATCCAATGTTAATGTGA
Protein sequence	>MS.gene90152.t1 MDLDFYMDFEDYFPSMVASLGAEGFIGELCNGFHLLMDVNKGLITFESLKMNCFMLGLEVRDEELMYMLMEGDLDGDGALNQMEFCILMFRLSPCLMDGPKMCTNPQGVNPMLM