Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062407.t1 | KEH16652.1 | 91 | 100 | 9 | 0 | 1 | 100 | 31 | 130 | 7.70E-24 | 119.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062407.t1 | A0A072TGZ8 | 91.0 | 100 | 9 | 0 | 1 | 100 | 31 | 130 | 5.5e-24 | 119.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051181 | MS.gene062407 | 0.802068 | 6.68E-49 | -1.69E-46 |
| MS.gene057049 | MS.gene062407 | 0.844489 | 7.32E-59 | -1.69E-46 |
| MS.gene057444 | MS.gene062407 | 0.80415 | 2.48E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene062407 | 0.808029 | 3.78E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene062407 | 0.809837 | 1.55E-50 | -1.69E-46 |
| MS.gene059557 | MS.gene062407 | 0.801924 | 7.16E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062407.t1 | MTR_0125s0060 | 92.000 | 100 | 8 | 0 | 1 | 100 | 31 | 130 | 1.02e-60 | 182 |
| MS.gene062407.t1 | MTR_2g035110 | 80.952 | 105 | 20 | 0 | 1 | 105 | 31 | 135 | 3.99e-53 | 162 |
| MS.gene062407.t1 | MTR_8g066845 | 58.491 | 106 | 24 | 3 | 1 | 105 | 1 | 87 | 4.00e-28 | 98.2 |
| MS.gene062407.t1 | MTR_4g012560 | 70.968 | 62 | 13 | 1 | 18 | 79 | 24 | 80 | 1.24e-19 | 76.6 |
| MS.gene062407.t1 | MTR_2g011670 | 54.000 | 50 | 23 | 0 | 37 | 86 | 18 | 67 | 4.73e-11 | 54.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAGAAGGGGCGCATATT+TGG | 0.259894 | 7.3:+74715556 | MS.gene062407:CDS |
| TGAATTGAAGTGTTCATATT+AGG | 0.299166 | 7.3:-74715781 | None:intergenic |
| CCATTATCACCTTCTCCTTC+CGG | 0.338322 | 7.3:-74715805 | None:intergenic |
| AAGGGGCGCATATTTGGATT+AGG | 0.373630 | 7.3:+74715562 | MS.gene062407:CDS |
| GATATTTATATGGAAATTGT+TGG | 0.391812 | 7.3:+74715526 | None:intergenic |
| GAGCGCTTGGAGCAAATGAT+TGG | 0.408396 | 7.3:+74715748 | MS.gene062407:CDS |
| CCGGAAGGAGAAGGTGATAA+TGG | 0.416904 | 7.3:+74715805 | MS.gene062407:CDS |
| AGAAACGCTAAAACTGAAGA+TGG | 0.499466 | 7.3:+74715726 | MS.gene062407:CDS |
| AAAACTGAAGATGGAGCGCT+TGG | 0.500382 | 7.3:+74715735 | MS.gene062407:CDS |
| TGTTGGAGGCATTAACAAGA+AGG | 0.515610 | 7.3:+74715543 | MS.gene062407:CDS |
| ATTTATATGGAAATTGTTGG+AGG | 0.522832 | 7.3:+74715529 | None:intergenic |
| AGCGCTTGGAGCAAATGATT+GGG | 0.526657 | 7.3:+74715749 | MS.gene062407:CDS |
| TATGAACACTTCAATTCAGC+CGG | 0.533653 | 7.3:+74715786 | MS.gene062407:CDS |
| TCAATTCAGCCGGAAGGAGA+AGG | 0.553755 | 7.3:+74715796 | MS.gene062407:CDS |
| GTTGGAGGCATTAACAAGAA+GGG | 0.555037 | 7.3:+74715544 | MS.gene062407:CDS |
| GCTACATCGGTAGAAATTGA+AGG | 0.569826 | 7.3:-74715625 | None:intergenic |
| TTGGAGGCATTAACAAGAAG+GGG | 0.580609 | 7.3:+74715545 | MS.gene062407:CDS |
| TCGGTAGAAATTGAAGGCGA+TGG | 0.604104 | 7.3:-74715619 | None:intergenic |
| AACACTTCAATTCAGCCGGA+AGG | 0.621409 | 7.3:+74715790 | MS.gene062407:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAATTGAAGTGTTCATATT+AGG | - | chr7.3:74715784-74715803 | None:intergenic | 25.0% |
| TTCGAAAAAAGTTGCTTCTA+TGG | + | chr7.3:74715651-74715670 | MS.gene062407:CDS | 30.0% | |
| !!! | TTTTTTCGAAGAAGCTACAT+CGG | - | chr7.3:74715641-74715660 | None:intergenic | 30.0% |
| AGAAACGCTAAAACTGAAGA+TGG | + | chr7.3:74715726-74715745 | MS.gene062407:CDS | 35.0% | |
| GAAAAAAGTTGCTTCTATGG+AGG | + | chr7.3:74715654-74715673 | MS.gene062407:CDS | 35.0% | |
| TATGAACACTTCAATTCAGC+CGG | + | chr7.3:74715786-74715805 | MS.gene062407:CDS | 35.0% | |
| GCTACATCGGTAGAAATTGA+AGG | - | chr7.3:74715628-74715647 | None:intergenic | 40.0% | |
| !! | GTTGGAGGCATTAACAAGAA+GGG | + | chr7.3:74715544-74715563 | MS.gene062407:CDS | 40.0% |
| !! | TGTTGGAGGCATTAACAAGA+AGG | + | chr7.3:74715543-74715562 | MS.gene062407:CDS | 40.0% |
| !! | TTGGAGGCATTAACAAGAAG+GGG | + | chr7.3:74715545-74715564 | MS.gene062407:CDS | 40.0% |
| AACACTTCAATTCAGCCGGA+AGG | + | chr7.3:74715790-74715809 | MS.gene062407:CDS | 45.0% | |
| AGCGCTTGGAGCAAATGATT+GGG | + | chr7.3:74715749-74715768 | MS.gene062407:CDS | 45.0% | |
| CCATTATCACCTTCTCCTTC+CGG | - | chr7.3:74715808-74715827 | None:intergenic | 45.0% | |
| TCGGTAGAAATTGAAGGCGA+TGG | - | chr7.3:74715622-74715641 | None:intergenic | 45.0% | |
| ! | AAAACTGAAGATGGAGCGCT+TGG | + | chr7.3:74715735-74715754 | MS.gene062407:CDS | 45.0% |
| ! | AACAAGAAGGGGCGCATATT+TGG | + | chr7.3:74715556-74715575 | MS.gene062407:CDS | 45.0% |
| ! | AAGGGGCGCATATTTGGATT+AGG | + | chr7.3:74715562-74715581 | MS.gene062407:CDS | 45.0% |
| CCGGAAGGAGAAGGTGATAA+TGG | + | chr7.3:74715805-74715824 | MS.gene062407:CDS | 50.0% | |
| GAGCGCTTGGAGCAAATGAT+TGG | + | chr7.3:74715748-74715767 | MS.gene062407:CDS | 50.0% | |
| TCAATTCAGCCGGAAGGAGA+AGG | + | chr7.3:74715796-74715815 | MS.gene062407:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 74715535 | 74715852 | 74715535 | ID=MS.gene062407 |
| chr7.3 | mRNA | 74715535 | 74715852 | 74715535 | ID=MS.gene062407.t1;Parent=MS.gene062407 |
| chr7.3 | exon | 74715535 | 74715852 | 74715535 | ID=MS.gene062407.t1.exon1;Parent=MS.gene062407.t1 |
| chr7.3 | CDS | 74715535 | 74715852 | 74715535 | ID=cds.MS.gene062407.t1;Parent=MS.gene062407.t1 |
| Gene Sequence |
| Protein sequence |
