Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057370.t1 | AFK36726.1 | 95.5 | 155 | 7 | 0 | 1 | 155 | 1 | 155 | 4.70E-78 | 300.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057370.t1 | Q9C768 | 53.8 | 156 | 66 | 4 | 2 | 155 | 4 | 155 | 1.4e-38 | 160.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057370.t1 | G7JAK1 | 95.5 | 155 | 7 | 0 | 1 | 155 | 1 | 155 | 3.4e-78 | 300.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055422 | MS.gene057370 | 0.812412 | 4.30E-51 | -1.69E-46 |
| MS.gene057370 | MS.gene36648 | 0.800031 | 1.74E-48 | -1.69E-46 |
| MS.gene057370 | MS.gene46177 | 0.800529 | 1.38E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057370.t1 | MTR_3g069690 | 95.484 | 155 | 7 | 0 | 1 | 155 | 1 | 155 | 6.18e-104 | 305 |
| MS.gene057370.t1 | MTR_3g069710 | 54.545 | 154 | 67 | 2 | 4 | 155 | 8 | 160 | 3.91e-54 | 177 |
| MS.gene057370.t1 | MTR_4g064987 | 45.963 | 161 | 81 | 2 | 1 | 155 | 1 | 161 | 3.69e-37 | 132 |
| MS.gene057370.t1 | MTR_4g064987 | 46.575 | 146 | 72 | 2 | 1 | 140 | 1 | 146 | 1.17e-33 | 123 |
| MS.gene057370.t1 | MTR_3g479620 | 33.125 | 160 | 95 | 2 | 8 | 155 | 6 | 165 | 8.40e-25 | 94.0 |
| MS.gene057370.t1 | MTR_3g479490 | 33.544 | 158 | 93 | 2 | 10 | 155 | 8 | 165 | 5.16e-23 | 94.4 |
| MS.gene057370.t1 | MTR_3g079480 | 35.625 | 160 | 91 | 3 | 8 | 155 | 21 | 180 | 3.51e-22 | 92.0 |
| MS.gene057370.t1 | MTR_4g094220 | 32.278 | 158 | 93 | 3 | 12 | 155 | 11 | 168 | 4.84e-22 | 91.3 |
| MS.gene057370.t1 | MTR_0036s0220 | 33.962 | 159 | 88 | 4 | 12 | 155 | 16 | 172 | 8.32e-22 | 90.9 |
| MS.gene057370.t1 | MTR_6g014040 | 34.545 | 165 | 88 | 5 | 10 | 155 | 12 | 175 | 1.73e-21 | 89.7 |
| MS.gene057370.t1 | MTR_5g464810 | 33.129 | 163 | 92 | 4 | 8 | 155 | 12 | 172 | 4.88e-21 | 88.6 |
| MS.gene057370.t1 | MTR_5g064240 | 33.129 | 163 | 92 | 4 | 8 | 155 | 12 | 172 | 4.88e-21 | 88.6 |
| MS.gene057370.t1 | MTR_5g464760 | 33.136 | 169 | 95 | 5 | 2 | 155 | 7 | 172 | 5.22e-21 | 88.6 |
| MS.gene057370.t1 | MTR_2g008210 | 34.545 | 165 | 91 | 5 | 4 | 155 | 7 | 167 | 3.83e-20 | 86.3 |
| MS.gene057370.t1 | MTR_6g014020 | 33.939 | 165 | 88 | 5 | 10 | 155 | 12 | 174 | 1.95e-19 | 84.0 |
| MS.gene057370.t1 | MTR_3g479520 | 33.333 | 159 | 92 | 4 | 10 | 155 | 17 | 174 | 2.20e-19 | 84.0 |
| MS.gene057370.t1 | MTR_6g014186 | 30.675 | 163 | 96 | 4 | 10 | 155 | 9 | 171 | 8.45e-19 | 82.0 |
| MS.gene057370.t1 | MTR_6g013440 | 30.588 | 170 | 101 | 3 | 3 | 155 | 6 | 175 | 1.38e-18 | 81.6 |
| MS.gene057370.t1 | MTR_6g014220 | 31.875 | 160 | 95 | 3 | 10 | 155 | 12 | 171 | 1.38e-18 | 81.6 |
| MS.gene057370.t1 | MTR_6g014250 | 31.875 | 160 | 95 | 3 | 10 | 155 | 12 | 171 | 2.66e-18 | 80.9 |
| MS.gene057370.t1 | MTR_6g013450 | 34.356 | 163 | 90 | 4 | 10 | 155 | 12 | 174 | 4.14e-18 | 80.5 |
| MS.gene057370.t1 | MTR_6g014290 | 29.630 | 162 | 100 | 2 | 8 | 155 | 10 | 171 | 6.90e-18 | 79.7 |
| MS.gene057370.t1 | MTR_0288s0020 | 31.928 | 166 | 95 | 4 | 8 | 155 | 10 | 175 | 1.11e-17 | 79.0 |
| MS.gene057370.t1 | MTR_6g014300 | 31.902 | 163 | 94 | 3 | 10 | 155 | 14 | 176 | 2.52e-17 | 78.2 |
| MS.gene057370.t1 | MTR_8g059425 | 32.099 | 162 | 93 | 4 | 10 | 155 | 12 | 172 | 2.91e-17 | 77.8 |
| MS.gene057370.t1 | MTR_0199s0010 | 30.189 | 159 | 98 | 2 | 10 | 155 | 12 | 170 | 8.79e-17 | 75.5 |
| MS.gene057370.t1 | MTR_3g008010 | 30.625 | 160 | 97 | 3 | 10 | 155 | 12 | 171 | 1.33e-16 | 75.9 |
| MS.gene057370.t1 | MTR_6g014170 | 31.875 | 160 | 95 | 3 | 10 | 155 | 12 | 171 | 1.86e-16 | 75.5 |
| MS.gene057370.t1 | MTR_8g007585 | 30.189 | 159 | 98 | 2 | 10 | 155 | 12 | 170 | 1.87e-16 | 75.5 |
| MS.gene057370.t1 | MTR_6g014195 | 28.571 | 168 | 103 | 3 | 5 | 155 | 7 | 174 | 1.94e-16 | 73.2 |
| MS.gene057370.t1 | MTR_6g014310 | 33.742 | 163 | 91 | 4 | 10 | 155 | 13 | 175 | 1.37e-15 | 73.2 |
| MS.gene057370.t1 | MTR_6g013960 | 29.448 | 163 | 98 | 3 | 10 | 155 | 12 | 174 | 7.88e-15 | 70.9 |
| MS.gene057370.t1 | MTR_6g014187 | 28.824 | 170 | 104 | 4 | 3 | 155 | 5 | 174 | 3.39e-14 | 68.9 |
| MS.gene057370.t1 | MTR_3g479470 | 31.677 | 161 | 96 | 5 | 8 | 155 | 13 | 172 | 3.88e-14 | 68.9 |
| MS.gene057370.t1 | MTR_6g014150 | 29.448 | 163 | 98 | 4 | 10 | 155 | 9 | 171 | 7.11e-14 | 68.2 |
| MS.gene057370.t1 | MTR_6g014270 | 27.011 | 174 | 107 | 5 | 1 | 155 | 1 | 173 | 1.44e-13 | 67.4 |
| MS.gene057370.t1 | MTR_7g011470 | 31.707 | 164 | 93 | 4 | 10 | 155 | 12 | 174 | 2.88e-13 | 66.2 |
| MS.gene057370.t1 | MTR_7g011470 | 31.707 | 164 | 93 | 4 | 10 | 155 | 12 | 174 | 2.88e-13 | 66.2 |
| MS.gene057370.t1 | MTR_6g014200 | 30.233 | 172 | 102 | 5 | 1 | 155 | 1 | 171 | 5.02e-13 | 65.9 |
| MS.gene057370.t1 | MTR_6g038300 | 30.818 | 159 | 95 | 5 | 8 | 153 | 11 | 167 | 8.50e-13 | 65.1 |
| MS.gene057370.t1 | MTR_6g013920 | 31.481 | 162 | 95 | 4 | 10 | 155 | 12 | 173 | 2.82e-12 | 63.5 |
| MS.gene057370.t1 | MTR_6g011340 | 33.129 | 163 | 92 | 4 | 10 | 155 | 12 | 174 | 5.81e-12 | 62.8 |
| MS.gene057370.t1 | MTR_6g038240 | 30.189 | 159 | 92 | 6 | 10 | 153 | 13 | 167 | 8.59e-12 | 62.0 |
| MS.gene057370.t1 | MTR_6g013900 | 29.630 | 162 | 98 | 4 | 10 | 155 | 12 | 173 | 1.24e-11 | 61.6 |
| MS.gene057370.t1 | MTR_6g014240 | 28.743 | 167 | 90 | 5 | 5 | 155 | 4 | 157 | 2.35e-11 | 60.8 |
| MS.gene057370.t1 | MTR_0475s0020 | 34.426 | 122 | 60 | 5 | 10 | 113 | 12 | 131 | 2.74e-11 | 59.3 |
| MS.gene057370.t1 | MTR_5g058320 | 29.012 | 162 | 84 | 4 | 10 | 155 | 12 | 158 | 2.82e-11 | 60.8 |
| MS.gene057370.t1 | MTR_8g088560 | 32.090 | 134 | 79 | 4 | 1 | 124 | 1 | 132 | 5.65e-11 | 59.7 |
| MS.gene057370.t1 | MTR_6g013990 | 30.000 | 170 | 102 | 4 | 3 | 155 | 5 | 174 | 8.81e-11 | 59.3 |
| MS.gene057370.t1 | MTR_8g083290 | 25.735 | 136 | 96 | 2 | 7 | 137 | 3 | 138 | 9.81e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057370.t1 | AT3G11340 | 53.846 | 156 | 66 | 4 | 2 | 155 | 4 | 155 | 7.51e-50 | 166 |
| MS.gene057370.t1 | AT5G05900 | 51.534 | 163 | 71 | 2 | 1 | 155 | 1 | 163 | 2.85e-44 | 151 |
| MS.gene057370.t1 | AT5G05860 | 49.682 | 157 | 76 | 3 | 1 | 154 | 1 | 157 | 3.13e-43 | 148 |
| MS.gene057370.t1 | AT3G55700 | 46.061 | 165 | 77 | 4 | 1 | 155 | 1 | 163 | 1.87e-40 | 141 |
| MS.gene057370.t1 | AT5G05880 | 48.428 | 159 | 78 | 2 | 1 | 155 | 1 | 159 | 2.26e-40 | 140 |
| MS.gene057370.t1 | AT5G05870 | 47.826 | 161 | 77 | 2 | 1 | 154 | 1 | 161 | 2.07e-37 | 133 |
| MS.gene057370.t1 | AT5G05890 | 49.080 | 163 | 75 | 2 | 1 | 155 | 1 | 163 | 2.48e-37 | 133 |
| MS.gene057370.t1 | AT3G55710 | 41.463 | 164 | 84 | 5 | 1 | 155 | 1 | 161 | 2.68e-29 | 111 |
| MS.gene057370.t1 | AT3G46670 | 40.667 | 150 | 85 | 3 | 6 | 152 | 7 | 155 | 4.32e-29 | 110 |
| MS.gene057370.t1 | AT5G59590 | 39.286 | 140 | 82 | 2 | 2 | 138 | 4 | 143 | 5.25e-29 | 110 |
| MS.gene057370.t1 | AT5G59580 | 38.806 | 134 | 78 | 3 | 8 | 138 | 9 | 141 | 1.13e-27 | 107 |
| MS.gene057370.t1 | AT3G46660 | 39.610 | 154 | 87 | 5 | 1 | 149 | 6 | 158 | 1.85e-27 | 106 |
| MS.gene057370.t1 | AT5G38010 | 37.267 | 161 | 94 | 4 | 2 | 155 | 4 | 164 | 7.65e-26 | 102 |
| MS.gene057370.t1 | AT3G46650 | 35.294 | 136 | 83 | 3 | 8 | 139 | 10 | 144 | 1.59e-24 | 98.2 |
| MS.gene057370.t1 | AT1G78270 | 33.125 | 160 | 91 | 3 | 10 | 155 | 15 | 172 | 3.70e-24 | 97.4 |
| MS.gene057370.t1 | AT5G38040 | 44.000 | 125 | 66 | 3 | 17 | 138 | 19 | 142 | 4.98e-24 | 97.1 |
| MS.gene057370.t1 | AT1G22370 | 33.540 | 161 | 91 | 3 | 9 | 155 | 14 | 172 | 5.95e-24 | 97.1 |
| MS.gene057370.t1 | AT3G46720 | 36.429 | 140 | 85 | 3 | 2 | 138 | 3 | 141 | 8.12e-24 | 96.3 |
| MS.gene057370.t1 | AT2G26480 | 36.709 | 158 | 92 | 3 | 1 | 155 | 1 | 153 | 1.99e-23 | 95.1 |
| MS.gene057370.t1 | AT1G22340 | 31.447 | 159 | 97 | 3 | 9 | 155 | 14 | 172 | 3.05e-23 | 94.7 |
| MS.gene057370.t1 | AT1G22400 | 30.189 | 159 | 99 | 2 | 9 | 155 | 14 | 172 | 5.38e-23 | 94.4 |
| MS.gene057370.t1 | AT1G22360 | 32.704 | 159 | 95 | 3 | 9 | 155 | 11 | 169 | 8.28e-23 | 93.6 |
| MS.gene057370.t1 | AT1G22360 | 32.704 | 159 | 95 | 3 | 9 | 155 | 11 | 169 | 8.66e-23 | 93.6 |
| MS.gene057370.t1 | AT1G22380 | 31.447 | 159 | 97 | 3 | 9 | 155 | 14 | 172 | 9.59e-23 | 93.6 |
| MS.gene057370.t1 | AT3G46690 | 32.258 | 155 | 100 | 3 | 2 | 152 | 3 | 156 | 1.34e-22 | 92.8 |
| MS.gene057370.t1 | AT3G46700 | 36.842 | 152 | 92 | 3 | 2 | 152 | 3 | 151 | 2.15e-21 | 89.7 |
| MS.gene057370.t1 | AT3G46680 | 36.111 | 144 | 84 | 5 | 1 | 139 | 4 | 144 | 1.90e-20 | 87.0 |
| MS.gene057370.t1 | AT5G37950 | 37.398 | 123 | 73 | 3 | 23 | 142 | 1 | 122 | 1.65e-17 | 78.2 |
| MS.gene057370.t1 | AT5G37950 | 37.097 | 124 | 74 | 3 | 23 | 143 | 1 | 123 | 1.80e-17 | 78.2 |
Find 27 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTCAAATCTCTTGAATT+TGG | 0.183072 | 3.1:-57055916 | MS.gene057370:CDS |
| ATTAAAACTTGTGTGAATTA+TGG | 0.230496 | 3.1:+57056007 | None:intergenic |
| GAGGGTAGTTTGAAGCGTTA+AGG | 0.346331 | 3.1:+57055982 | None:intergenic |
| AGGGTAGTTTGAAGCGTTAA+GGG | 0.351785 | 3.1:+57055983 | None:intergenic |
| ACTTGTGTGAATTATGGTTA+TGG | 0.371137 | 3.1:+57056013 | None:intergenic |
| GCACAAATCCTTTACTCAAA+TGG | 0.375219 | 3.1:-57056041 | MS.gene057370:CDS |
| TGTGTCCTTGTAATGGTAAA+GGG | 0.397029 | 3.1:+57056084 | None:intergenic |
| ATGATTCACTTAAACCATCT+TGG | 0.400138 | 3.1:+57055940 | None:intergenic |
| TAGTTGTGTGAAGCCTTTCA+AGG | 0.425424 | 3.1:-57055877 | MS.gene057370:CDS |
| CTTATCCCTTTACCATTACA+AGG | 0.430466 | 3.1:-57056089 | MS.gene057370:CDS |
| AATACAAACTAACCTTGTAT+AGG | 0.433125 | 3.1:+57055663 | None:intergenic |
| TCTGATATCAAGCAAGCAAT+AGG | 0.439864 | 3.1:+57055816 | None:intergenic |
| ATGTGTCCTTGTAATGGTAA+AGG | 0.449617 | 3.1:+57056083 | None:intergenic |
| AACTTCTGTTGTATCCAAGA+TGG | 0.470730 | 3.1:-57055954 | MS.gene057370:CDS |
| ATTTGTGCAAGTTGAAGCAT+TGG | 0.476729 | 3.1:+57056056 | None:intergenic |
| CTACAAGCTTTAAGCTTCCA+AGG | 0.483823 | 3.1:-57055764 | MS.gene057370:CDS |
| AAGCCTTTCAAGGAGTGCTT+AGG | 0.491426 | 3.1:-57055867 | MS.gene057370:CDS |
| GGATTTATGTGTCCTTGTAA+TGG | 0.493771 | 3.1:+57056077 | None:intergenic |
| CCAGTTCTCAAAACAAGCCT+TGG | 0.504265 | 3.1:+57055747 | None:intergenic |
| TTACCTAAGCACTCCTTGAA+AGG | 0.512347 | 3.1:+57055864 | None:intergenic |
| AATGGGTATTTGCCTATACA+AGG | 0.521663 | 3.1:-57055675 | None:intergenic |
| CACATCAATGAAGGAAACAA+AGG | 0.550670 | 3.1:-57056126 | None:intergenic |
| ACATCAATGAAGGAAACAAA+GGG | 0.564697 | 3.1:-57056125 | None:intergenic |
| AAAGGCAGCAAAAGCAACAA+AGG | 0.584196 | 3.1:+57055713 | None:intergenic |
| TACAACAGAAGTTGAAGTGA+GGG | 0.594460 | 3.1:+57055964 | None:intergenic |
| ACTACTATGTGATGTATCTG+AGG | 0.619410 | 3.1:-57055841 | MS.gene057370:CDS |
| ATACAACAGAAGTTGAAGTG+AGG | 0.677720 | 3.1:+57055963 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAAACTTGTGTGAATTA+TGG | + | chr3.1:57055791-57055810 | None:intergenic | 20.0% |
| !! | ATTTTCTCTCAAAAATGGAA+AGG | + | chr3.1:57056103-57056122 | None:intergenic | 25.0% |
| !!! | TTCCATTTTTGAGAGAAAAT+GGG | - | chr3.1:57056103-57056122 | MS.gene057370:CDS | 25.0% |
| !!! | TTTCCATTTTTGAGAGAAAA+TGG | - | chr3.1:57056102-57056121 | MS.gene057370:CDS | 25.0% |
| ACTTGTGTGAATTATGGTTA+TGG | + | chr3.1:57055785-57055804 | None:intergenic | 30.0% | |
| ATGATTCACTTAAACCATCT+TGG | + | chr3.1:57055858-57055877 | None:intergenic | 30.0% | |
| TGCTTCAAATCTCTTGAATT+TGG | - | chr3.1:57055879-57055898 | MS.gene057370:CDS | 30.0% | |
| TGGAAAAACCATTTGAGTAA+AGG | + | chr3.1:57055765-57055784 | None:intergenic | 30.0% | |
| ! | TACCCATTTTCTCTCAAAAA+TGG | + | chr3.1:57056108-57056127 | None:intergenic | 30.0% |
| AACTTCTGTTGTATCCAAGA+TGG | - | chr3.1:57055841-57055860 | MS.gene057370:CDS | 35.0% | |
| ACTACTATGTGATGTATCTG+AGG | - | chr3.1:57055954-57055973 | MS.gene057370:CDS | 35.0% | |
| ATACAACAGAAGTTGAAGTG+AGG | + | chr3.1:57055835-57055854 | None:intergenic | 35.0% | |
| ATGTGTCCTTGTAATGGTAA+AGG | + | chr3.1:57055715-57055734 | None:intergenic | 35.0% | |
| CTTATCCCTTTACCATTACA+AGG | - | chr3.1:57055706-57055725 | MS.gene057370:CDS | 35.0% | |
| GCACAAATCCTTTACTCAAA+TGG | - | chr3.1:57055754-57055773 | MS.gene057370:CDS | 35.0% | |
| GGATTTATGTGTCCTTGTAA+TGG | + | chr3.1:57055721-57055740 | None:intergenic | 35.0% | |
| TACAACAGAAGTTGAAGTGA+GGG | + | chr3.1:57055834-57055853 | None:intergenic | 35.0% | |
| TCTGATATCAAGCAAGCAAT+AGG | + | chr3.1:57055982-57056001 | None:intergenic | 35.0% | |
| TGTGTCCTTGTAATGGTAAA+GGG | + | chr3.1:57055714-57055733 | None:intergenic | 35.0% | |
| ! | ATTTGTGCAAGTTGAAGCAT+TGG | + | chr3.1:57055742-57055761 | None:intergenic | 35.0% |
| AAAGGCAGCAAAAGCAACAA+AGG | + | chr3.1:57056085-57056104 | None:intergenic | 40.0% | |
| TAGTTGTGTGAAGCCTTTCA+AGG | - | chr3.1:57055918-57055937 | MS.gene057370:CDS | 40.0% | |
| TTACCTAAGCACTCCTTGAA+AGG | + | chr3.1:57055934-57055953 | None:intergenic | 40.0% | |
| ! | AGGGTAGTTTGAAGCGTTAA+GGG | + | chr3.1:57055815-57055834 | None:intergenic | 40.0% |
| !! | CTACAAGCTTTAAGCTTCCA+AGG | - | chr3.1:57056031-57056050 | MS.gene057370:CDS | 40.0% |
| CCAGTTCTCAAAACAAGCCT+TGG | + | chr3.1:57056051-57056070 | None:intergenic | 45.0% | |
| ! | GAGGGTAGTTTGAAGCGTTA+AGG | + | chr3.1:57055816-57055835 | None:intergenic | 45.0% |
| !! | AAGCCTTTCAAGGAGTGCTT+AGG | - | chr3.1:57055928-57055947 | MS.gene057370:CDS | 45.0% |
| !!! | AGGCTTGTTTTGAGAACTGG+TGG | - | chr3.1:57056051-57056070 | MS.gene057370:CDS | 45.0% |
| !!! | CCAAGGCTTGTTTTGAGAAC+TGG | - | chr3.1:57056048-57056067 | MS.gene057370:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 57055676 | 57056141 | 57055676 | ID=MS.gene057370 |
| chr3.1 | mRNA | 57055676 | 57056141 | 57055676 | ID=MS.gene057370.t1;Parent=MS.gene057370 |
| chr3.1 | exon | 57055676 | 57056141 | 57055676 | ID=MS.gene057370.t1.exon1;Parent=MS.gene057370.t1 |
| chr3.1 | CDS | 57055676 | 57056141 | 57055676 | ID=cds.MS.gene057370.t1;Parent=MS.gene057370.t1 |
| Gene Sequence |
| Protein sequence |
