Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057581.t1 | RHN44295.1 | 68.4 | 117 | 17 | 2 | 73 | 189 | 92 | 188 | 1.30E-29 | 139.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057581.t1 | G7L237 | 68.4 | 117 | 17 | 2 | 73 | 189 | 118 | 214 | 9.3e-30 | 139.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057576 | MS.gene057581 | 0.838542 | 2.70E-57 | -1.69E-46 |
| MS.gene057580 | MS.gene057581 | 0.840744 | 7.23E-58 | -1.69E-46 |
| MS.gene057581 | MS.gene057584 | 0.813081 | 3.07E-51 | -1.69E-46 |
| MS.gene057581 | MS.gene057586 | 0.89549 | 9.12E-76 | -1.69E-46 |
| MS.gene057581 | MS.gene057588 | 0.83215 | 1.11E-55 | -1.69E-46 |
| MS.gene057581 | MS.gene49520 | 0.850025 | 2.22E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057581.t1 | MTR_7g011390 | 62.553 | 235 | 21 | 4 | 1 | 189 | 1 | 214 | 2.47e-73 | 220 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGGCTGCTGCTGGCATCCA+AGG | 0.270786 | 7.2:+85888631 | None:intergenic |
| ATCCAACGGCTGGCTGCTGC+TGG | 0.310560 | 7.2:+85888622 | None:intergenic |
| CAGTTTCTTCTTCTTAGACT+TGG | 0.357409 | 7.2:+85889926 | None:intergenic |
| GTGCACATGCCAACACAATA+TGG | 0.384828 | 7.2:-85889042 | MS.gene057581:CDS |
| TGAGGATAAAGCTGCTGTTC+AGG | 0.399727 | 7.2:-85888567 | MS.gene057581:CDS |
| TGGCTGCTGCTGGCATCCAA+GGG | 0.406161 | 7.2:+85888632 | None:intergenic |
| GTGAAGCTGTATCATCTTCA+TGG | 0.410616 | 7.2:+85889076 | None:intergenic |
| TTATGATCAGATTTATCGTC+AGG | 0.438391 | 7.2:-85889759 | MS.gene057581:intron |
| GCTGTTCAGGAGGGGTGTAT+TGG | 0.455461 | 7.2:-85888554 | MS.gene057581:CDS |
| ACCTCCTTGCCATATTGTGT+TGG | 0.459988 | 7.2:+85889033 | None:intergenic |
| GGCATGTGCACAGGTTGAAT+TGG | 0.460863 | 7.2:+85889054 | None:intergenic |
| CTCTTACTCTAAATTCACTC+TGG | 0.470546 | 7.2:-85889445 | MS.gene057581:CDS |
| TGCCAGCAGCAGCCAGCCGT+TGG | 0.479830 | 7.2:-85888624 | MS.gene057581:CDS |
| TATTGTGTTGGCATGTGCAC+AGG | 0.498215 | 7.2:+85889045 | None:intergenic |
| TGAAAATGATATGAAGCCCT+TGG | 0.509252 | 7.2:-85888648 | MS.gene057581:CDS |
| TCGTTCGTTCCCATGGGTGG+AGG | 0.527930 | 7.2:-85889975 | None:intergenic |
| ACAGGCCCGCAAAGCAAAAG+AGG | 0.538823 | 7.2:-85889480 | MS.gene057581:CDS |
| TGTTGAGCTCATCCAACGGC+TGG | 0.538954 | 7.2:+85888612 | None:intergenic |
| GCCCAGTCGATTAGCAAAAG+AGG | 0.543880 | 7.2:-85889522 | MS.gene057581:CDS |
| AACTCGTTCGTTCCCATGGG+TGG | 0.547111 | 7.2:-85889978 | None:intergenic |
| CTGTGTTTGTAATGTTTGCA+AGG | 0.567137 | 7.2:+85889357 | None:intergenic |
| TCGTGTGTGGGCAGTAAAGA+CGG | 0.585395 | 7.2:+85889402 | None:intergenic |
| GATAAAGCTGCTGTTCAGGA+GGG | 0.586121 | 7.2:-85888563 | MS.gene057581:CDS |
| GACTTGTTGAGCTCATCCAA+CGG | 0.603464 | 7.2:+85888608 | None:intergenic |
| GTGTTTCTCTAGTCGTGTGT+GGG | 0.609787 | 7.2:+85889390 | None:intergenic |
| AGTGTTTCTCTAGTCGTGTG+TGG | 0.609826 | 7.2:+85889389 | None:intergenic |
| GGATAAAGCTGCTGTTCAGG+AGG | 0.611925 | 7.2:-85888564 | MS.gene057581:CDS |
| ATAAAGCTGCTGTTCAGGAG+GGG | 0.613356 | 7.2:-85888562 | MS.gene057581:CDS |
| GAACAGCAGCTTTATCCTCA+AGG | 0.621167 | 7.2:+85888570 | None:intergenic |
| TCTTACTCTAAATTCACTCT+GGG | 0.627636 | 7.2:-85889444 | MS.gene057581:CDS |
| GACACAAAACGTTAGCCTTG+AGG | 0.630577 | 7.2:-85888585 | MS.gene057581:CDS |
| CATGCCAACACAATATGGCA+AGG | 0.661413 | 7.2:-85889037 | MS.gene057581:CDS |
| ACGGTGCATTGCATCAGCAG+TGG | 0.666153 | 7.2:+85889421 | None:intergenic |
| GCCAACACAATATGGCAAGG+AGG | 0.737684 | 7.2:-85889034 | MS.gene057581:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACTAAATGTTATGAAAAT+TGG | + | chr7.2:85888665-85888684 | None:intergenic | 15.0% |
| !! | TATGATATGAATTGTTAATT+TGG | - | chr7.2:85888860-85888879 | MS.gene057581:intron | 15.0% |
| !! | TTATAAGAAATTAAGCAAAA+GGG | - | chr7.2:85889796-85889815 | MS.gene057581:intron | 15.0% |
| !!! | ATTTTTAATTATGTATTTGC+AGG | - | chr7.2:85889829-85889848 | MS.gene057581:intron | 15.0% |
| !! | AATTTCTTATAAGTAAGGAA+AGG | + | chr7.2:85889788-85889807 | None:intergenic | 20.0% |
| !! | CTTATAAGAAATTAAGCAAA+AGG | - | chr7.2:85889795-85889814 | MS.gene057581:intron | 20.0% |
| !! | TGCTTAATTTCTTATAAGTA+AGG | + | chr7.2:85889793-85889812 | None:intergenic | 20.0% |
| !!! | ATGAATTGTTAATTTGGTTA+TGG | - | chr7.2:85888866-85888885 | MS.gene057581:intron | 20.0% |
| !!! | CATAGCTTTTCTTTAAAATT+GGG | - | chr7.2:85889696-85889715 | MS.gene057581:intron | 20.0% |
| !!! | TAAAAAAAAAGTCTTGTAGA+AGG | + | chr7.2:85889503-85889522 | None:intergenic | 20.0% |
| !!! | TTTTAACTAACTTGTTATGT+TGG | - | chr7.2:85888780-85888799 | MS.gene057581:intron | 20.0% |
| !! | GTTTTCTTAAAAATTGCTCT+AGG | - | chr7.2:85889179-85889198 | MS.gene057581:intron | 25.0% |
| !!! | ACTTGGATTTCTTTTTTGTT+CGG | + | chr7.2:85888567-85888586 | None:intergenic | 25.0% |
| !!! | CCAATTTTAAAGAAAAGCTA+TGG | + | chr7.2:85889698-85889717 | None:intergenic | 25.0% |
| !!! | CCATAGCTTTTCTTTAAAAT+TGG | - | chr7.2:85889695-85889714 | MS.gene057581:intron | 25.0% |
| !!! | GCAATTTTTAAGAAAACCTA+CGG | + | chr7.2:85889176-85889195 | None:intergenic | 25.0% |
| AGATGAAAAGTGAATAGAAG+TGG | + | chr7.2:85889630-85889649 | None:intergenic | 30.0% | |
| ATAGATAGATAGATAGAGAG+AGG | + | chr7.2:85888615-85888634 | None:intergenic | 30.0% | |
| CTTCTTACTAATACACCAAA+TGG | - | chr7.2:85889722-85889741 | MS.gene057581:intron | 30.0% | |
| GAATAGAAGTGGTATACTTA+TGG | + | chr7.2:85889619-85889638 | None:intergenic | 30.0% | |
| TCTTACTCTAAATTCACTCT+GGG | - | chr7.2:85889063-85889082 | MS.gene057581:CDS | 30.0% | |
| TCTTGCTCAAAAAAAACTAC+AGG | - | chr7.2:85889009-85889028 | MS.gene057581:intron | 30.0% | |
| TTATGATCAGATTTATCGTC+AGG | - | chr7.2:85888748-85888767 | MS.gene057581:intron | 30.0% | |
| CAAAAAAGTAGCAGAAGAAG+AGG | - | chr7.2:85888960-85888979 | MS.gene057581:intron | 35.0% | |
| CAACTAATATCCAGACACTA+AGG | + | chr7.2:85889570-85889589 | None:intergenic | 35.0% | |
| CAGTTTCTTCTTCTTAGACT+TGG | + | chr7.2:85888584-85888603 | None:intergenic | 35.0% | |
| CTCTTACTCTAAATTCACTC+TGG | - | chr7.2:85889062-85889081 | MS.gene057581:CDS | 35.0% | |
| CTGTGTTTGTAATGTTTGCA+AGG | + | chr7.2:85889153-85889172 | None:intergenic | 35.0% | |
| TGAAAATGATATGAAGCCCT+TGG | - | chr7.2:85889859-85889878 | MS.gene057581:intron | 35.0% | |
| ! | ATGGATTGACATGAATGTTC+AGG | + | chr7.2:85889600-85889619 | None:intergenic | 35.0% |
| ! | CATGATGATGATGATGATCT+TGG | - | chr7.2:85888905-85888924 | MS.gene057581:intron | 35.0% |
| ! | GAAAAAGCGTATCATCTTCA+TGG | + | chr7.2:85889290-85889309 | None:intergenic | 35.0% |
| ! | TTTTGTAATCTGTATGCAGC+TGG | - | chr7.2:85889263-85889282 | MS.gene057581:intron | 35.0% |
| !! | AAGCACGTTGTTTCTTAGTA+AGG | + | chr7.2:85889220-85889239 | None:intergenic | 35.0% |
| !! | CGTTGTTTCTTAGTAAGGAA+AGG | + | chr7.2:85889215-85889234 | None:intergenic | 35.0% |
| !!! | CTCTTCTTCTGCTACTTTTT+TGG | + | chr7.2:85888962-85888981 | None:intergenic | 35.0% |
| AACAGGAGATAGGTTTATGC+AGG | + | chr7.2:85889537-85889556 | None:intergenic | 40.0% | |
| AACATTACAAACACAGCCGT+AGG | - | chr7.2:85889157-85889176 | MS.gene057581:intron | 40.0% | |
| ACTAAGGCAGTTTCATGAAC+AGG | + | chr7.2:85889554-85889573 | None:intergenic | 40.0% | |
| CAGTTTCATGAACAGGAGAT+AGG | + | chr7.2:85889547-85889566 | None:intergenic | 40.0% | |
| GATAAGAAGACAGTCCTAGA+GGG | + | chr7.2:85889766-85889785 | None:intergenic | 40.0% | |
| GTGAAGCTGTATCATCTTCA+TGG | + | chr7.2:85889434-85889453 | None:intergenic | 40.0% | |
| ! | ATGATCTTGGCTCTCTGTAT+AGG | - | chr7.2:85888918-85888937 | MS.gene057581:intron | 40.0% |
| !! | CTGTTCTGTACTGACTAATC+TGG | - | chr7.2:85888811-85888830 | MS.gene057581:intron | 40.0% |
| !! | TCTGTACTGACTAATCTGGA+TGG | - | chr7.2:85888815-85888834 | MS.gene057581:intron | 40.0% |
| AAAGCTACAAACACAGCCGT+AGG | - | chr7.2:85889321-85889340 | MS.gene057581:intron | 45.0% | |
| ACCTCCTTGCCATATTGTGT+TGG | + | chr7.2:85889477-85889496 | None:intergenic | 45.0% | |
| AGTGTTTCTCTAGTCGTGTG+TGG | + | chr7.2:85889121-85889140 | None:intergenic | 45.0% | |
| ATAAAGCTGCTGTTCAGGAG+GGG | - | chr7.2:85889945-85889964 | MS.gene057581:CDS | 45.0% | |
| CATGAAACTGCCTTAGTGTC+TGG | - | chr7.2:85889557-85889576 | MS.gene057581:intron | 45.0% | |
| CATGCCAACACAATATGGCA+AGG | - | chr7.2:85889470-85889489 | MS.gene057581:CDS | 45.0% | |
| GATAAAGCTGCTGTTCAGGA+GGG | - | chr7.2:85889944-85889963 | MS.gene057581:CDS | 45.0% | |
| GGATAAGAAGACAGTCCTAG+AGG | + | chr7.2:85889767-85889786 | None:intergenic | 45.0% | |
| GTGCACATGCCAACACAATA+TGG | - | chr7.2:85889465-85889484 | MS.gene057581:CDS | 45.0% | |
| GTGTTTCTCTAGTCGTGTGT+GGG | + | chr7.2:85889120-85889139 | None:intergenic | 45.0% | |
| TGAGGATAAAGCTGCTGTTC+AGG | - | chr7.2:85889940-85889959 | MS.gene057581:CDS | 45.0% | |
| ! | AGCCTCTTTTGCTAATCGAC+TGG | + | chr7.2:85888990-85889009 | None:intergenic | 45.0% |
| ! | GAACAGCAGCTTTATCCTCA+AGG | + | chr7.2:85889940-85889959 | None:intergenic | 45.0% |
| ! | GCCTCTTTTGCTAATCGACT+GGG | + | chr7.2:85888989-85889008 | None:intergenic | 45.0% |
| ! | TATTGTGTTGGCATGTGCAC+AGG | + | chr7.2:85889465-85889484 | None:intergenic | 45.0% |
| !! | GACACAAAACGTTAGCCTTG+AGG | - | chr7.2:85889922-85889941 | MS.gene057581:intron | 45.0% |
| !! | GACTTGTTGAGCTCATCCAA+CGG | + | chr7.2:85889902-85889921 | None:intergenic | 45.0% |
| !! | TACAAAATAAAAATAAAAAA+TGG | + | chr7.2:85888705-85888724 | None:intergenic | 5.0% |
| CACCAAATGGAGAGAGCCTT+AGG | - | chr7.2:85889735-85889754 | MS.gene057581:intron | 50.0% | |
| GCCAACACAATATGGCAAGG+AGG | - | chr7.2:85889473-85889492 | MS.gene057581:CDS | 50.0% | |
| GCCCAGTCGATTAGCAAAAG+AGG | - | chr7.2:85888985-85889004 | MS.gene057581:intron | 50.0% | |
| GGATAAAGCTGCTGTTCAGG+AGG | - | chr7.2:85889943-85889962 | MS.gene057581:CDS | 50.0% | |
| TCGTGTGTGGGCAGTAAAGA+CGG | + | chr7.2:85889108-85889127 | None:intergenic | 50.0% | |
| TGCCTAAGGCTCTCTCCATT+TGG | + | chr7.2:85889740-85889759 | None:intergenic | 50.0% | |
| ! | CTGCTGCCTCTTTTGCTTTG+CGG | + | chr7.2:85889036-85889055 | None:intergenic | 50.0% |
| ! | GGCATGTGCACAGGTTGAAT+TGG | + | chr7.2:85889456-85889475 | None:intergenic | 50.0% |
| ! | TGCTGCCTCTTTTGCTTTGC+GGG | + | chr7.2:85889035-85889054 | None:intergenic | 50.0% |
| ACAGGCCCGCAAAGCAAAAG+AGG | - | chr7.2:85889027-85889046 | MS.gene057581:intron | 55.0% | |
| ACGGTGCATTGCATCAGCAG+TGG | + | chr7.2:85889089-85889108 | None:intergenic | 55.0% | |
| AGCCTTAGGCAATGCCCTCT+AGG | - | chr7.2:85889749-85889768 | MS.gene057581:intron | 55.0% | |
| TGTTGAGCTCATCCAACGGC+TGG | + | chr7.2:85889898-85889917 | None:intergenic | 55.0% | |
| ! | GCTGTTCAGGAGGGGTGTAT+TGG | - | chr7.2:85889953-85889972 | MS.gene057581:CDS | 55.0% |
| !! | GTCCTAGAGGGCATTGCCTA+AGG | + | chr7.2:85889754-85889773 | None:intergenic | 55.0% |
| TGGCTGCTGCTGGCATCCAA+GGG | + | chr7.2:85889878-85889897 | None:intergenic | 60.0% | |
| CTGGCTGCTGCTGGCATCCA+AGG | + | chr7.2:85889879-85889898 | None:intergenic | 65.0% | |
| ! | ATCCAACGGCTGGCTGCTGC+TGG | + | chr7.2:85889888-85889907 | None:intergenic | 65.0% |
| TGCCAGCAGCAGCCAGCCGT+TGG | - | chr7.2:85889883-85889902 | MS.gene057581:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 85888544 | 85889985 | 85888544 | ID=MS.gene057581 |
| chr7.2 | mRNA | 85888544 | 85889985 | 85888544 | ID=MS.gene057581.t1;Parent=MS.gene057581 |
| chr7.2 | exon | 85889925 | 85889985 | 85889925 | ID=MS.gene057581.t1.exon1;Parent=MS.gene057581.t1 |
| chr7.2 | CDS | 85889925 | 85889985 | 85889925 | ID=cds.MS.gene057581.t1;Parent=MS.gene057581.t1 |
| chr7.2 | exon | 85889760 | 85889803 | 85889760 | ID=MS.gene057581.t1.exon2;Parent=MS.gene057581.t1 |
| chr7.2 | CDS | 85889760 | 85889803 | 85889760 | ID=cds.MS.gene057581.t1;Parent=MS.gene057581.t1 |
| chr7.2 | exon | 85889355 | 85889558 | 85889355 | ID=MS.gene057581.t1.exon3;Parent=MS.gene057581.t1 |
| chr7.2 | CDS | 85889355 | 85889558 | 85889355 | ID=cds.MS.gene057581.t1;Parent=MS.gene057581.t1 |
| chr7.2 | exon | 85889191 | 85889247 | 85889191 | ID=MS.gene057581.t1.exon4;Parent=MS.gene057581.t1 |
| chr7.2 | CDS | 85889191 | 85889247 | 85889191 | ID=cds.MS.gene057581.t1;Parent=MS.gene057581.t1 |
| chr7.2 | exon | 85889035 | 85889103 | 85889035 | ID=MS.gene057581.t1.exon5;Parent=MS.gene057581.t1 |
| chr7.2 | CDS | 85889035 | 85889103 | 85889035 | ID=cds.MS.gene057581.t1;Parent=MS.gene057581.t1 |
| chr7.2 | exon | 85888544 | 85888678 | 85888544 | ID=MS.gene057581.t1.exon6;Parent=MS.gene057581.t1 |
| chr7.2 | CDS | 85888544 | 85888678 | 85888544 | ID=cds.MS.gene057581.t1;Parent=MS.gene057581.t1 |
| Gene Sequence |
| Protein sequence |
