Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49520.t1 | ABD32736.1 | 74.9 | 215 | 16 | 3 | 24 | 220 | 189 | 383 | 2.00E-37 | 166 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49520.t1 | G7L237 | 74.9 | 215 | 16 | 3 | 24 | 220 | 23 | 217 | 1.4e-37 | 166.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057576 | MS.gene49520 | 0.905942 | 2.52E-80 | -1.69E-46 |
| MS.gene057577 | MS.gene49520 | 0.886286 | 3.90E-72 | -1.69E-46 |
| MS.gene057580 | MS.gene49520 | 0.86411 | 1.54E-64 | -1.69E-46 |
| MS.gene057581 | MS.gene49520 | 0.850025 | 2.22E-60 | -1.69E-46 |
| MS.gene057584 | MS.gene49520 | 0.899982 | 1.15E-77 | -1.69E-46 |
| MS.gene057586 | MS.gene49520 | 0.928317 | 3.43E-92 | -1.69E-46 |
| MS.gene057588 | MS.gene49520 | 0.909513 | 5.24E-82 | -1.69E-46 |
| MS.gene057591 | MS.gene49520 | 0.858798 | 6.43E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49520.t1 | MTR_7g011390 | 77.311 | 238 | 15 | 3 | 1 | 220 | 1 | 217 | 1.96e-86 | 255 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGGCTGCTGCTGGCATCCA+AGG | 0.270786 | 7.4:+87990011 | None:intergenic |
| ATCCAACGGCTGGCTGCTGC+TGG | 0.310560 | 7.4:+87990002 | None:intergenic |
| CAGTTTCTTCTTCTTAGACT+TGG | 0.357409 | 7.4:+87991510 | None:intergenic |
| GTGCACATGCCAACACAATA+TGG | 0.384828 | 7.4:-87990479 | MS.gene49520:CDS |
| TGAGGATAAAGCTGCTGTTC+AGG | 0.399727 | 7.4:-87989947 | MS.gene49520:CDS |
| TGGCTGCTGCTGGCATCCAA+GGG | 0.406161 | 7.4:+87990012 | None:intergenic |
| GTGAAGCTGTATCATCTTCA+TGG | 0.410616 | 7.4:+87990513 | None:intergenic |
| GGTCCAATATCTAGAGTAGT+TGG | 0.419554 | 7.4:+87989905 | None:intergenic |
| CTCTTATTCTAAATTCACTC+TGG | 0.436351 | 7.4:-87990902 | MS.gene49520:CDS |
| TTATGATCAGATTTATCGTC+AGG | 0.438391 | 7.4:-87991348 | MS.gene49520:intron |
| ACCTCCTTGCCATATTGTGT+TGG | 0.459988 | 7.4:+87990470 | None:intergenic |
| GGCATGTGCACAGGTTGAAT+TGG | 0.460863 | 7.4:+87990491 | None:intergenic |
| ATAAAGCTGCTGTTCAGGAG+GGG | 0.477624 | 7.4:-87989942 | MS.gene49520:CDS |
| TGCCAGCAGCAGCCAGCCGT+TGG | 0.479830 | 7.4:-87990004 | MS.gene49520:CDS |
| TATTGTGTTGGCATGTGCAC+AGG | 0.498215 | 7.4:+87990482 | None:intergenic |
| TGAAAATGATATGAAGCCCT+TGG | 0.509252 | 7.4:-87990028 | MS.gene49520:CDS |
| TAAAGCTGCTGTTCAGGAGG+GGG | 0.518520 | 7.4:-87989941 | MS.gene49520:CDS |
| TCGTTCGTTCCCATGGGTGG+AGG | 0.527930 | 7.4:-87991559 | None:intergenic |
| AGCCTCTTCTGCTAATCGAC+GGG | 0.530366 | 7.4:+87991031 | None:intergenic |
| GTCCAATATCTAGAGTAGTT+GGG | 0.530987 | 7.4:+87989906 | None:intergenic |
| GATAAAGCTGCTGTTCAGGA+GGG | 0.535418 | 7.4:-87989943 | MS.gene49520:CDS |
| ACAGGCCCGCAAAGCAAAAG+AGG | 0.538823 | 7.4:-87990937 | MS.gene49520:CDS |
| TGTTGAGCTCATCCAACGGC+TGG | 0.538954 | 7.4:+87989992 | None:intergenic |
| AACTCGTTCGTTCCCATGGG+TGG | 0.547111 | 7.4:-87991562 | None:intergenic |
| AGCCCGTCGATTAGCAAAAG+AGG | 0.550604 | 7.4:-87990979 | MS.gene49520:CDS |
| AGCCCGTCGATTAGCAGAAG+AGG | 0.554670 | 7.4:-87991033 | MS.gene49520:CDS |
| TCTTATTCTAAATTCACTCT+GGG | 0.558794 | 7.4:-87990901 | MS.gene49520:CDS |
| CTGTGTTTGTAATGTTTGCA+AGG | 0.567137 | 7.4:+87990814 | None:intergenic |
| TCGTGTGTGGGCAGTAAAGA+CGG | 0.585395 | 7.4:+87990859 | None:intergenic |
| GAGCCTCTTCTGCTAATCGA+CGG | 0.589148 | 7.4:+87991030 | None:intergenic |
| GACTTGTTGAGCTCATCCAA+CGG | 0.593483 | 7.4:+87989988 | None:intergenic |
| AAAGCTGCTGTTCAGGAGGG+GGG | 0.608878 | 7.4:-87989940 | MS.gene49520:CDS |
| GTGTTTCTCTAGTCGTGTGT+GGG | 0.609787 | 7.4:+87990847 | None:intergenic |
| AGTGTTTCTCTAGTCGTGTG+TGG | 0.609826 | 7.4:+87990846 | None:intergenic |
| GGATAAAGCTGCTGTTCAGG+AGG | 0.611925 | 7.4:-87989944 | MS.gene49520:CDS |
| GAACAGCAGCTTTATCCTCA+AGG | 0.621167 | 7.4:+87989950 | None:intergenic |
| AACACAAAACGTTAGCCTTG+AGG | 0.622562 | 7.4:-87989965 | MS.gene49520:CDS |
| CATGCCAACACAATATGGCA+AGG | 0.661413 | 7.4:-87990474 | MS.gene49520:CDS |
| ACGGTGCATTGCATCAGCAG+TGG | 0.666153 | 7.4:+87990878 | None:intergenic |
| GCCAACACAATATGGCAAGG+AGG | 0.737684 | 7.4:-87990471 | MS.gene49520:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGATATGAATTGTTAATT+TGG | - | chr7.4:87990241-87990260 | MS.gene49520:intron | 15.0% |
| !! | TTATAAGAAATTAAGCAAAA+GGG | - | chr7.4:87991371-87991390 | MS.gene49520:CDS | 15.0% |
| !!! | ATTTTTAATTATGTATTTGC+AGG | - | chr7.4:87991404-87991423 | MS.gene49520:intron | 15.0% |
| !! | AATTTCTTATAAGTAAGGAA+AGG | + | chr7.4:87991363-87991382 | None:intergenic | 20.0% |
| !! | ATAAAATGATACTACTAATC+TGG | - | chr7.4:87990192-87990211 | MS.gene49520:intron | 20.0% |
| !! | ATACAGATTACAAAATTTCA+CGG | + | chr7.4:87990756-87990775 | None:intergenic | 20.0% |
| !! | CTAGTAAATGTTATGAAAAT+TGG | + | chr7.4:87990028-87990047 | None:intergenic | 20.0% |
| !! | CTTATAAGAAATTAAGCAAA+AGG | - | chr7.4:87991370-87991389 | MS.gene49520:CDS | 20.0% |
| !! | TGCTTAATTTCTTATAAGTA+AGG | + | chr7.4:87991368-87991387 | None:intergenic | 20.0% |
| !!! | AATAAAAAAAGTCTTGTAGA+AGG | + | chr7.4:87991021-87991040 | None:intergenic | 20.0% |
| !!! | ATGAATTGTTAATTTGGTTA+TGG | - | chr7.4:87990247-87990266 | MS.gene49520:intron | 20.0% |
| !!! | CATAGCTTTTCTTTAAAATT+GGG | - | chr7.4:87991271-87991290 | MS.gene49520:intron | 20.0% |
| !!! | TTTTAACTAACTTGTTATGT+TGG | - | chr7.4:87990146-87990165 | MS.gene49520:intron | 20.0% |
| ! | ACCTACAAATACAAATACAA+TGG | + | chr7.4:87990367-87990386 | None:intergenic | 25.0% |
| ! | ATAAATAGATAGATAGAGAG+AGG | + | chr7.4:87989986-87990005 | None:intergenic | 25.0% |
| ! | TAAAAAAAAAGCAGAAGAAG+AGG | - | chr7.4:87990459-87990478 | MS.gene49520:intron | 25.0% |
| ! | TCCATTGTATTTGTATTTGT+AGG | - | chr7.4:87990363-87990382 | MS.gene49520:intron | 25.0% |
| ! | TCTTATTCTAAATTCACTCT+GGG | - | chr7.4:87990561-87990580 | MS.gene49520:intron | 25.0% |
| !! | GTTTTCTTAAAAATTGCTCT+AGG | - | chr7.4:87990677-87990696 | MS.gene49520:CDS | 25.0% |
| !!! | ACTTGGATTTCTTTTTTGTT+CGG | + | chr7.4:87989938-87989957 | None:intergenic | 25.0% |
| !!! | CCAATTTTAAAGAAAAGCTA+TGG | + | chr7.4:87991273-87991292 | None:intergenic | 25.0% |
| !!! | CCATAGCTTTTCTTTAAAAT+TGG | - | chr7.4:87991270-87991289 | MS.gene49520:intron | 25.0% |
| !!! | GCAATTTTTAAGAAAACCTA+CGG | + | chr7.4:87990674-87990693 | None:intergenic | 25.0% |
| AATGATACTACTAATCTGGA+TGG | - | chr7.4:87990196-87990215 | MS.gene49520:intron | 30.0% | |
| AGATGAAAAGTGAATAGAAG+TGG | + | chr7.4:87991205-87991224 | None:intergenic | 30.0% | |
| CTCTTATTCTAAATTCACTC+TGG | - | chr7.4:87990560-87990579 | MS.gene49520:intron | 30.0% | |
| CTTCTTACTAATACACCAAA+TGG | - | chr7.4:87991297-87991316 | MS.gene49520:intron | 30.0% | |
| GAATAGAAGTGGTATACTTA+TGG | + | chr7.4:87991194-87991213 | None:intergenic | 30.0% | |
| TCTTGCTCAAAAAAAACTAC+AGG | - | chr7.4:87990507-87990526 | MS.gene49520:CDS | 30.0% | |
| TTATGATCAGATTTATCGTC+AGG | - | chr7.4:87990114-87990133 | MS.gene49520:intron | 30.0% | |
| !! | AAGCAAGTTGTTTCTTAGTA+AGG | + | chr7.4:87990882-87990901 | None:intergenic | 30.0% |
| !! | AGTTGTTTCTTAGTAAGGAA+AGG | + | chr7.4:87990877-87990896 | None:intergenic | 30.0% |
| ATAAGAAGAAAGTCCTAGAG+GGG | + | chr7.4:87991340-87991359 | None:intergenic | 35.0% | |
| CAAAAAAGTAGCAGAAGAAG+AGG | - | chr7.4:87990405-87990424 | MS.gene49520:intron | 35.0% | |
| CAGTTTCTTCTTCTTAGACT+TGG | + | chr7.4:87989955-87989974 | None:intergenic | 35.0% | |
| CTGTGTTTGTAATGTTTGCA+AGG | + | chr7.4:87990651-87990670 | None:intergenic | 35.0% | |
| GATAAGAAGAAAGTCCTAGA+GGG | + | chr7.4:87991341-87991360 | None:intergenic | 35.0% | |
| TGAAAATGATATGAAGCCCT+TGG | - | chr7.4:87991434-87991453 | MS.gene49520:intron | 35.0% | |
| ! | ATGGATTGACATGAATGTTC+AGG | + | chr7.4:87991175-87991194 | None:intergenic | 35.0% |
| ! | GAAAAAGCGTATCATCTTCA+TGG | + | chr7.4:87990788-87990807 | None:intergenic | 35.0% |
| ! | GATGATGATGATGATGATCT+TGG | - | chr7.4:87990295-87990314 | MS.gene49520:intron | 35.0% |
| ! | TTTTGTAATCTGTATGCAGC+TGG | - | chr7.4:87990761-87990780 | MS.gene49520:intron | 35.0% |
| !! | AAGCACGTTGTTTCTTAGTA+AGG | + | chr7.4:87990718-87990737 | None:intergenic | 35.0% |
| !! | CGTTGTTTCTTAGTAAGGAA+AGG | + | chr7.4:87990713-87990732 | None:intergenic | 35.0% |
| !!! | CTCTTCTTCTGCTACTTTTT+TGG | + | chr7.4:87990407-87990426 | None:intergenic | 35.0% |
| AACATTACAAACACAGCCGT+AGG | - | chr7.4:87990655-87990674 | MS.gene49520:CDS | 40.0% | |
| GGATAAGAAGAAAGTCCTAG+AGG | + | chr7.4:87991342-87991361 | None:intergenic | 40.0% | |
| GTGAAGCTGTATCATCTTCA+TGG | + | chr7.4:87990952-87990971 | None:intergenic | 40.0% | |
| ! | ATGATCTTGGCTCTCTGTAT+AGG | - | chr7.4:87990308-87990327 | MS.gene49520:intron | 40.0% |
| !! | AACACAAAACGTTAGCCTTG+AGG | - | chr7.4:87991497-87991516 | MS.gene49520:intron | 40.0% |
| AAAGCTACAAACACAGCCGT+AGG | - | chr7.4:87990819-87990838 | MS.gene49520:CDS | 45.0% | |
| ACCTCCTTGCCATATTGTGT+TGG | + | chr7.4:87990995-87991014 | None:intergenic | 45.0% | |
| AGCAGGAGATAGGTTTATGC+AGG | + | chr7.4:87991053-87991072 | None:intergenic | 45.0% | |
| AGTGTTTCTCTAGTCGTGTG+TGG | + | chr7.4:87990619-87990638 | None:intergenic | 45.0% | |
| ATAAAGCTGCTGTTCAGGAG+GGG | - | chr7.4:87991520-87991539 | MS.gene49520:CDS | 45.0% | |
| CAGTTTCATGAGCAGGAGAT+AGG | + | chr7.4:87991063-87991082 | None:intergenic | 45.0% | |
| CATGCCAACACAATATGGCA+AGG | - | chr7.4:87990988-87991007 | MS.gene49520:CDS | 45.0% | |
| GATAAAGCTGCTGTTCAGGA+GGG | - | chr7.4:87991519-87991538 | MS.gene49520:CDS | 45.0% | |
| GTGCACATGCCAACACAATA+TGG | - | chr7.4:87990983-87991002 | MS.gene49520:CDS | 45.0% | |
| GTGTTTCTCTAGTCGTGTGT+GGG | + | chr7.4:87990618-87990637 | None:intergenic | 45.0% | |
| TGAGGATAAAGCTGCTGTTC+AGG | - | chr7.4:87991515-87991534 | MS.gene49520:CDS | 45.0% | |
| ! | AGCCTCTTTTGCTAATCGAC+GGG | + | chr7.4:87990488-87990507 | None:intergenic | 45.0% |
| ! | GAACAGCAGCTTTATCCTCA+AGG | + | chr7.4:87991515-87991534 | None:intergenic | 45.0% |
| ! | GAGCCTCTTTTGCTAATCGA+CGG | + | chr7.4:87990489-87990508 | None:intergenic | 45.0% |
| ! | TATTGTGTTGGCATGTGCAC+AGG | + | chr7.4:87990983-87991002 | None:intergenic | 45.0% |
| !! | GACTTGTTGAGCTCATCCAA+CGG | + | chr7.4:87991477-87991496 | None:intergenic | 45.0% |
| !! | TACAAAATAAAAATAAAAAA+TGG | + | chr7.4:87990071-87990090 | None:intergenic | 5.0% |
| AGCCTCTTCTGCTAATCGAC+GGG | + | chr7.4:87990434-87990453 | None:intergenic | 50.0% | |
| CACACACACACACACACACT+AGG | + | chr7.4:87991086-87991105 | None:intergenic | 50.0% | |
| CACCAAATGGAGAGAGCCTT+AGG | - | chr7.4:87991310-87991329 | MS.gene49520:intron | 50.0% | |
| CACTAGGCAGTTTCATGAGC+AGG | + | chr7.4:87991070-87991089 | None:intergenic | 50.0% | |
| GAGCCTCTTCTGCTAATCGA+CGG | + | chr7.4:87990435-87990454 | None:intergenic | 50.0% | |
| GCCAACACAATATGGCAAGG+AGG | - | chr7.4:87990991-87991010 | MS.gene49520:CDS | 50.0% | |
| GGATAAAGCTGCTGTTCAGG+AGG | - | chr7.4:87991518-87991537 | MS.gene49520:CDS | 50.0% | |
| TAAAGCTGCTGTTCAGGAGG+GGG | - | chr7.4:87991521-87991540 | MS.gene49520:CDS | 50.0% | |
| TCGTGTGTGGGCAGTAAAGA+CGG | + | chr7.4:87990606-87990625 | None:intergenic | 50.0% | |
| TGCCTAAGGCTCTCTCCATT+TGG | + | chr7.4:87991315-87991334 | None:intergenic | 50.0% | |
| TGTGTCTGTCTGTCTGTGTC+TGG | - | chr7.4:87991132-87991151 | MS.gene49520:intron | 50.0% | |
| ! | AGCCCGTCGATTAGCAAAAG+AGG | - | chr7.4:87990483-87990502 | MS.gene49520:CDS | 50.0% |
| ! | CTGCTGCCTCTTTTGCTTTG+CGG | + | chr7.4:87990534-87990553 | None:intergenic | 50.0% |
| ! | GGCATGTGCACAGGTTGAAT+TGG | + | chr7.4:87990974-87990993 | None:intergenic | 50.0% |
| ! | TGCTGCCTCTTTTGCTTTGC+GGG | + | chr7.4:87990533-87990552 | None:intergenic | 50.0% |
| AAAGCTGCTGTTCAGGAGGG+GGG | - | chr7.4:87991522-87991541 | MS.gene49520:CDS | 55.0% | |
| ACAGGCCCGCAAAGCAAAAG+AGG | - | chr7.4:87990525-87990544 | MS.gene49520:intron | 55.0% | |
| ACGGTGCATTGCATCAGCAG+TGG | + | chr7.4:87990587-87990606 | None:intergenic | 55.0% | |
| AGCCTTAGGCAATCCCCTCT+AGG | - | chr7.4:87991324-87991343 | MS.gene49520:intron | 55.0% | |
| GTCCTAGAGGGGATTGCCTA+AGG | + | chr7.4:87991329-87991348 | None:intergenic | 55.0% | |
| TGTTGAGCTCATCCAACGGC+TGG | + | chr7.4:87991473-87991492 | None:intergenic | 55.0% | |
| ! | AGCCCGTCGATTAGCAGAAG+AGG | - | chr7.4:87990429-87990448 | MS.gene49520:intron | 55.0% |
| TGGCTGCTGCTGGCATCCAA+GGG | + | chr7.4:87991453-87991472 | None:intergenic | 60.0% | |
| ! | GCTGTTCAGGAGGGGGGTAT+TGG | - | chr7.4:87991528-87991547 | MS.gene49520:CDS | 60.0% |
| CTGGCTGCTGCTGGCATCCA+AGG | + | chr7.4:87991454-87991473 | None:intergenic | 65.0% | |
| ! | ATCCAACGGCTGGCTGCTGC+TGG | + | chr7.4:87991463-87991482 | None:intergenic | 65.0% |
| TGCCAGCAGCAGCCAGCCGT+TGG | - | chr7.4:87991458-87991477 | MS.gene49520:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 87989915 | 87991569 | 87989915 | ID=MS.gene49520 |
| chr7.4 | mRNA | 87989915 | 87991569 | 87989915 | ID=MS.gene49520.t1;Parent=MS.gene49520 |
| chr7.4 | exon | 87991509 | 87991569 | 87991509 | ID=MS.gene49520.t1.exon1;Parent=MS.gene49520.t1 |
| chr7.4 | CDS | 87991509 | 87991569 | 87991509 | ID=cds.MS.gene49520.t1;Parent=MS.gene49520.t1 |
| chr7.4 | exon | 87991349 | 87991392 | 87991349 | ID=MS.gene49520.t1.exon2;Parent=MS.gene49520.t1 |
| chr7.4 | CDS | 87991349 | 87991392 | 87991349 | ID=cds.MS.gene49520.t1;Parent=MS.gene49520.t1 |
| chr7.4 | exon | 87990812 | 87991099 | 87990812 | ID=MS.gene49520.t1.exon3;Parent=MS.gene49520.t1 |
| chr7.4 | CDS | 87990812 | 87991099 | 87990812 | ID=cds.MS.gene49520.t1;Parent=MS.gene49520.t1 |
| chr7.4 | exon | 87990648 | 87990704 | 87990648 | ID=MS.gene49520.t1.exon4;Parent=MS.gene49520.t1 |
| chr7.4 | CDS | 87990648 | 87990704 | 87990648 | ID=cds.MS.gene49520.t1;Parent=MS.gene49520.t1 |
| chr7.4 | exon | 87990472 | 87990540 | 87990472 | ID=MS.gene49520.t1.exon5;Parent=MS.gene49520.t1 |
| chr7.4 | CDS | 87990472 | 87990540 | 87990472 | ID=cds.MS.gene49520.t1;Parent=MS.gene49520.t1 |
| chr7.4 | exon | 87989915 | 87990058 | 87989915 | ID=MS.gene49520.t1.exon6;Parent=MS.gene49520.t1 |
| chr7.4 | CDS | 87989915 | 87990058 | 87989915 | ID=cds.MS.gene49520.t1;Parent=MS.gene49520.t1 |
| Gene Sequence |
| Protein sequence |
