AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057632

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057632.t1 MTR_4g063220 99.324 148 1 0 1 148 1 148 3.84e-102 289
MS.gene057632.t1 MTR_4g063990 99.324 148 1 0 1 148 1 148 3.84e-102 289
MS.gene057632.t1 MTR_4g063200 99.324 148 1 0 1 148 1 148 3.84e-102 289
MS.gene057632.t1 MTR_4g064020 99.324 148 1 0 1 148 1 148 3.84e-102 289
MS.gene057632.t1 MTR_4g071180 99.324 148 1 0 1 148 1 148 8.19e-102 288
MS.gene057632.t1 MTR_4g070240 99.324 148 1 0 1 148 1 148 8.19e-102 288
MS.gene057632.t1 MTR_4g070245 97.973 148 3 0 1 148 1 148 4.03e-100 284
MS.gene057632.t1 MTR_4g063240 98.649 148 1 1 1 148 1 147 1.11e-99 283
MS.gene057632.t1 MTR_4g063275 96.622 148 5 0 1 148 1 148 8.15e-99 280
MS.gene057632.t1 MTR_4g064983 98.611 144 2 0 1 144 1 144 7.90e-97 279
MS.gene057632.t1 MTR_4g088025 93.919 148 4 1 1 148 1 143 7.97e-89 255
MS.gene057632.t1 MTR_1g090780 88.514 148 6 3 1 148 1 137 1.62e-78 229
MS.gene057632.t1 MTR_1g492910 87.838 148 7 3 1 148 1 137 8.01e-78 227
MS.gene057632.t1 MTR_1g068600 92.793 111 4 1 38 148 30 136 3.27e-64 192
MS.gene057632.t1 MTR_7g099960 92.793 111 5 1 38 148 31 138 6.03e-64 192
MS.gene057632.t1 MTR_2g084480 61.798 89 34 0 59 147 136 224 4.66e-37 127
MS.gene057632.t1 MTR_8g069990 68.817 93 21 3 15 107 4 88 3.43e-34 115
MS.gene057632.t1 MTR_4g063270 90.196 51 5 0 86 136 1 51 5.07e-27 95.9
MS.gene057632.t1 MTR_7g114580 90.244 41 4 0 108 148 11 51 9.18e-20 77.4
MS.gene057632.t1 MTR_4g032777 36.792 106 63 2 31 132 5 110 1.69e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057632.t1 AT3G45980 88.742 151 13 2 1 148 1 150 1.41e-83 242
MS.gene057632.t1 AT2G28720 86.093 151 18 1 1 148 1 151 9.31e-81 235
MS.gene057632.t1 AT1G07790 86.928 153 10 3 1 148 1 148 7.39e-80 233
MS.gene057632.t1 AT5G59910 91.176 136 9 2 13 148 18 150 2.55e-77 226
MS.gene057632.t1 AT3G46030 94.444 126 6 1 23 148 21 145 8.32e-76 222
MS.gene057632.t1 AT2G37470 82.432 148 15 3 1 147 1 138 5.77e-71 210
MS.gene057632.t1 AT3G53650 81.208 149 16 3 1 148 1 138 4.43e-69 205
MS.gene057632.t1 AT5G22880 87.586 145 16 2 4 148 3 145 4.09e-67 200
MS.gene057632.t1 AT5G02570 79.730 148 14 1 1 148 1 132 9.33e-67 199
MS.gene057632.t1 AT3G09480 84.127 126 16 1 23 148 5 126 2.05e-62 187
MS.gene057632.t1 AT1G08170 61.176 85 33 0 61 145 151 235 2.87e-37 127

Find 46 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTAAGAGCTTGTGAATT+TGG 0.203095 4.2:-36647117 None:intergenic
GGTAGCTTCTTTCCAGCCTT+TGG 0.299167 4.2:-36646783 None:intergenic
GCTTGTTCTGCCCGGTGAAT+TGG 0.304089 4.2:+36647058 MS.gene057632:CDS
TTGCTTGAGATACCAATGTC+TGG 0.335174 4.2:-36646909 None:intergenic
GCTGGCTTCTTCTCTCCCTT+TGG 0.362568 4.2:-36646696 None:intergenic
CTTGTTGTACCTAGCGAGTC+TGG 0.384866 4.2:-36646994 None:intergenic
TCTGCGGGTTTCTTCTCTGC+TGG 0.399254 4.2:-36646714 None:intergenic
CTTCTTCTTGTCTCCGGCAG+CGG 0.399506 4.2:-36646820 None:intergenic
GCCTTTGGCTTCTTCTCCGC+CGG 0.402654 4.2:-36646768 None:intergenic
GCAGCGGAACCACCTTCCTT+TGG 0.428768 4.2:-36646804 None:intergenic
GTGGATTTCTTCTCCTCTGC+GGG 0.431011 4.2:-36646729 None:intergenic
ATACAAGATCTACATCTTCA+AGG 0.451479 4.2:+36646869 MS.gene057632:CDS
AAGGAAGGTGGTTCCGCTGC+CGG 0.458129 4.2:+36646807 MS.gene057632:CDS
ACCCTCAGAAACAGCATGCT+TGG 0.462043 4.2:-36647081 None:intergenic
AGCATGCTTGGCCAATTCAC+CGG 0.471051 4.2:-36647069 None:intergenic
GGGAAATTCAGACCGCGGTC+AGG 0.471115 4.2:+36647036 MS.gene057632:CDS
TCTCTTCTTCTTCTTGTCTC+CGG 0.474508 4.2:-36646826 None:intergenic
TGTGAATTTGGTCACAGCCT+TGG 0.493306 4.2:-36647105 None:intergenic
AATTCCTTCAATGGCGCCAA+AGG 0.495983 4.2:+36646680 None:intergenic
GCGGAGAAGAAGCCAAAGGC+TGG 0.504484 4.2:+36646771 MS.gene057632:CDS
GCATGCTTGGCCAATTCACC+GGG 0.525255 4.2:-36647068 None:intergenic
TGCTTGAGATACCAATGTCT+GGG 0.532971 4.2:-36646908 None:intergenic
TGGTATCTCAAGCAAAGCCA+TGG 0.533917 4.2:+36646917 MS.gene057632:CDS
GCCAAGCATGCTGTTTCTGA+GGG 0.538001 4.2:+36647080 MS.gene057632:CDS
GGCCAAGCATGCTGTTTCTG+AGG 0.538906 4.2:+36647079 MS.gene057632:CDS
CACAGTCGCTGAGAAAGCTC+CGG 0.552253 4.2:+36646749 MS.gene057632:CDS
GCGGTCAGGCTTGTTCTGCC+CGG 0.555879 4.2:+36647050 MS.gene057632:CDS
ATTCCTTCAATGGCGCCAAA+GGG 0.555903 4.2:+36646681 None:intergenic
ATTTCCCTCGAAGTGATCGT+CGG 0.569915 4.2:-36647020 None:intergenic
TGCTGTTTCTGAGGGTACCA+AGG 0.572597 4.2:+36647088 MS.gene057632:CDS
GAAACTGTTCATGATTCCCA+TGG 0.578509 4.2:-36646934 None:intergenic
AGAAGCCGACGATCACTTCG+AGG 0.586465 4.2:+36647015 MS.gene057632:CDS
AAGAATCTTCCAGACTCGCT+AGG 0.594905 4.2:+36646985 MS.gene057632:CDS
AAGCAAGTTCACCCAGACAT+TGG 0.601452 4.2:+36646897 MS.gene057632:CDS
TGTGGATTTCTTCTCCTCTG+CGG 0.602479 4.2:-36646730 None:intergenic
GGAAAGAAGCTACCAAAGGA+AGG 0.624050 4.2:+36646792 MS.gene057632:CDS
CGGAGCTTTCTCAGCGACTG+TGG 0.628186 4.2:-36646748 None:intergenic
GGCTGGAAAGAAGCTACCAA+AGG 0.634160 4.2:+36646788 MS.gene057632:CDS
GGTATCTCAAGCAAAGCCAT+GGG 0.654333 4.2:+36646918 MS.gene057632:CDS
AGTCGCTGAGAAAGCTCCGG+CGG 0.656886 4.2:+36646752 MS.gene057632:CDS
GAAGCCGACGATCACTTCGA+GGG 0.660226 4.2:+36647016 MS.gene057632:CDS
AAGAAGCTACCAAAGGAAGG+TGG 0.666846 4.2:+36646795 MS.gene057632:CDS
TCCGGCGGAGAAGAAGCCAA+AGG 0.682003 4.2:+36646767 MS.gene057632:CDS
GGGCAGAACAAGCCTGACCG+CGG 0.695125 4.2:-36647048 None:intergenic
AGCAGAGAAGAAACCCGCAG+AGG 0.698236 4.2:+36646716 MS.gene057632:CDS
TTCGAGGGAAATTCAGACCG+CGG 0.709650 4.2:+36647031 MS.gene057632:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATACAAGATCTACATCTTCA+AGG + chr4.2:36646869-36646888 MS.gene057632:CDS 30.0%
GAAACTGTTCATGATTCCCA+TGG - chr4.2:36646937-36646956 None:intergenic 40.0%
TCTCTTCTTCTTCTTGTCTC+CGG - chr4.2:36646829-36646848 None:intergenic 40.0%
TGCTTGAGATACCAATGTCT+GGG - chr4.2:36646911-36646930 None:intergenic 40.0%
TTGCTTGAGATACCAATGTC+TGG - chr4.2:36646912-36646931 None:intergenic 40.0%
AAGAAGCTACCAAAGGAAGG+TGG + chr4.2:36646795-36646814 MS.gene057632:CDS 45.0%
AAGAATCTTCCAGACTCGCT+AGG + chr4.2:36646985-36647004 MS.gene057632:CDS 45.0%
AAGCAAGTTCACCCAGACAT+TGG + chr4.2:36646897-36646916 MS.gene057632:CDS 45.0%
ATTTCCCTCGAAGTGATCGT+CGG - chr4.2:36647023-36647042 None:intergenic 45.0%
GGAAAGAAGCTACCAAAGGA+AGG + chr4.2:36646792-36646811 MS.gene057632:CDS 45.0%
GGTATCTCAAGCAAAGCCAT+GGG + chr4.2:36646918-36646937 MS.gene057632:CDS 45.0%
TGGTATCTCAAGCAAAGCCA+TGG + chr4.2:36646917-36646936 MS.gene057632:CDS 45.0%
TGTGAATTTGGTCACAGCCT+TGG - chr4.2:36647108-36647127 None:intergenic 45.0%
TGTGGATTTCTTCTCCTCTG+CGG - chr4.2:36646733-36646752 None:intergenic 45.0%
ACCCTCAGAAACAGCATGCT+TGG - chr4.2:36647084-36647103 None:intergenic 50.0%
AGCATGCTTGGCCAATTCAC+CGG - chr4.2:36647072-36647091 None:intergenic 50.0%
GGCTGGAAAGAAGCTACCAA+AGG + chr4.2:36646788-36646807 MS.gene057632:CDS 50.0%
GGTAGCTTCTTTCCAGCCTT+TGG - chr4.2:36646786-36646805 None:intergenic 50.0%
GTGGATTTCTTCTCCTCTGC+GGG - chr4.2:36646732-36646751 None:intergenic 50.0%
TTCGAGGGAAATTCAGACCG+CGG + chr4.2:36647031-36647050 MS.gene057632:CDS 50.0%
! CTTGTTGTACCTAGCGAGTC+TGG - chr4.2:36646997-36647016 None:intergenic 50.0%
!! GCCAAGCATGCTGTTTCTGA+GGG + chr4.2:36647080-36647099 MS.gene057632:CDS 50.0%
!! TGCTGTTTCTGAGGGTACCA+AGG + chr4.2:36647088-36647107 MS.gene057632:CDS 50.0%
AGAAGCCGACGATCACTTCG+AGG + chr4.2:36647015-36647034 MS.gene057632:CDS 55.0%
AGCAGAGAAGAAACCCGCAG+AGG + chr4.2:36646716-36646735 MS.gene057632:CDS 55.0%
CACAGTCGCTGAGAAAGCTC+CGG + chr4.2:36646749-36646768 MS.gene057632:CDS 55.0%
CTTCTTCTTGTCTCCGGCAG+CGG - chr4.2:36646823-36646842 None:intergenic 55.0%
GAAGCCGACGATCACTTCGA+GGG + chr4.2:36647016-36647035 MS.gene057632:CDS 55.0%
GCATGCTTGGCCAATTCACC+GGG - chr4.2:36647071-36647090 None:intergenic 55.0%
GCTTGTTCTGCCCGGTGAAT+TGG + chr4.2:36647058-36647077 MS.gene057632:CDS 55.0%
TCTGCGGGTTTCTTCTCTGC+TGG - chr4.2:36646717-36646736 None:intergenic 55.0%
! GCTGGCTTCTTCTCTCCCTT+TGG - chr4.2:36646699-36646718 None:intergenic 55.0%
!! GGCCAAGCATGCTGTTTCTG+AGG + chr4.2:36647079-36647098 MS.gene057632:CDS 55.0%
AAGGAAGGTGGTTCCGCTGC+CGG + chr4.2:36646807-36646826 MS.gene057632:CDS 60.0%
AGTCGCTGAGAAAGCTCCGG+CGG + chr4.2:36646752-36646771 MS.gene057632:CDS 60.0%
CGGAGCTTTCTCAGCGACTG+TGG - chr4.2:36646751-36646770 None:intergenic 60.0%
GCAGCGGAACCACCTTCCTT+TGG - chr4.2:36646807-36646826 None:intergenic 60.0%
GCGGAGAAGAAGCCAAAGGC+TGG + chr4.2:36646771-36646790 MS.gene057632:CDS 60.0%
GGGAAATTCAGACCGCGGTC+AGG + chr4.2:36647036-36647055 MS.gene057632:CDS 60.0%
TCCGGCGGAGAAGAAGCCAA+AGG + chr4.2:36646767-36646786 MS.gene057632:CDS 60.0%
! GCCTTTGGCTTCTTCTCCGC+CGG - chr4.2:36646771-36646790 None:intergenic 60.0%
GGGCAGAACAAGCCTGACCG+CGG - chr4.2:36647051-36647070 None:intergenic 65.0%
! GCGGTCAGGCTTGTTCTGCC+CGG + chr4.2:36647050-36647069 MS.gene057632:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 36646690 36647136 36646690 ID=MS.gene057632
chr4.2 mRNA 36646690 36647136 36646690 ID=MS.gene057632.t1;Parent=MS.gene057632
chr4.2 exon 36646690 36647136 36646690 ID=MS.gene057632.t1.exon1;Parent=MS.gene057632.t1
chr4.2 CDS 36646690 36647136 36646690 ID=cds.MS.gene057632.t1;Parent=MS.gene057632.t1
Gene Sequence

>MS.gene057632

ATGGCGCCAAAGGGAGAGAAGAAGCCAGCAGAGAAGAAACCCGCAGAGGAGAAGAAATCCACAGTCGCTGAGAAAGCTCCGGCGGAGAAGAAGCCAAAGGCTGGAAAGAAGCTACCAAAGGAAGGTGGTTCCGCTGCCGGAGACAAGAAGAAGAAGAGAAGCAAGAAGAGCGTTGAAACATACAAGATCTACATCTTCAAGGTTCTGAAGCAAGTTCACCCAGACATTGGTATCTCAAGCAAAGCCATGGGAATCATGAACAGTTTCATTAACGACATCTTCGAGAAGCTCGCTCAAGAATCTTCCAGACTCGCTAGGTACAACAAGAAGCCGACGATCACTTCGAGGGAAATTCAGACCGCGGTCAGGCTTGTTCTGCCCGGTGAATTGGCCAAGCATGCTGTTTCTGAGGGTACCAAGGCTGTGACCAAATTCACAAGCTCTTAG
Protein sequence

>MS.gene057632.t1

MAPKGEKKPAEKKPAEEKKSTVAEKAPAEKKPKAGKKLPKEGGSAAGDKKKKRSKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS