AlfalfaGEDB | MS.gene062889

M. sativa cultivar XinJiangDaYe / MS.gene062889

KEGG: K11251
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF00125.27 PF16211.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062889.t1	XP_003607007.1	98	152	1	1	1	150	1	152	8.30E-72	279.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062889.t1	Q1S053	98.0	152	1	1	1	150	1	152	2.0e-74	279.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062889.t1	G7JIM1	98.0	152	1	1	1	150	1	152	6.0e-72	279.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051263	MS.gene062889	0.836138	1.12E-56	-1.69E-46
MS.gene055957	MS.gene062889	0.868556	5.98E-66	-1.69E-46
MS.gene056041	MS.gene062889	0.817922	2.56E-52	-1.69E-46
MS.gene05640	MS.gene062889	0.804523	2.07E-49	-1.69E-46
MS.gene057458	MS.gene062889	0.825973	3.50E-54	-1.69E-46
MS.gene057632	MS.gene062889	0.81893	1.52E-52	-1.69E-46
MS.gene060565	MS.gene062889	0.805918	1.06E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062889.t1	MTR_4g071150	98.026	152	1	1	1	150	1	152	9.78e-101	285
MS.gene062889.t1	MTR_2g096610	87.333	150	17	1	1	150	1	148	4.41e-83	241
MS.gene062889.t1	MTR_4g064005	94.000	150	7	1	1	150	1	148	1.32e-82	239
MS.gene062889.t1	MTR_4g063410	94.000	150	7	1	1	150	1	148	2.70e-82	239
MS.gene062889.t1	MTR_2g096570	91.558	154	8	2	1	150	1	153	5.64e-82	238
MS.gene062889.t1	MTR_4g063280	92.424	132	9	1	20	150	14	145	2.84e-81	236
MS.gene062889.t1	MTR_4g064967	93.333	150	8	1	1	150	1	148	2.98e-80	234
MS.gene062889.t1	MTR_8g086640	96.212	132	5	0	1	132	1	132	4.27e-75	221
MS.gene062889.t1	MTR_7g113160	79.699	133	26	1	10	142	13	144	5.01e-71	210
MS.gene062889.t1	MTR_7g108320	75.000	128	26	3	4	130	6	128	7.58e-60	182
MS.gene062889.t1	MTR_7g114060	74.219	128	29	1	3	130	2	125	7.85e-60	182
MS.gene062889.t1	MTR_7g114040	74.219	128	29	1	3	130	2	125	7.85e-60	182
MS.gene062889.t1	MTR_7g093910	73.228	127	31	1	4	130	5	128	9.33e-60	181
MS.gene062889.t1	MTR_2g082510	70.543	129	38	0	11	139	3	131	3.21e-59	180
MS.gene062889.t1	MTR_2g082220	73.950	119	31	0	11	129	3	121	1.65e-57	176
MS.gene062889.t1	MTR_6g005150	62.992	127	33	3	24	149	26	139	3.16e-47	150
MS.gene062889.t1	MTR_4g063300	75.238	105	21	1	45	149	10	109	3.34e-46	146
MS.gene062889.t1	MTR_7g114050	68.644	118	22	3	17	130	10	116	2.19e-45	145
MS.gene062889.t1	MTR_1g011750	60.714	112	42	2	21	131	23	133	3.59e-36	121
MS.gene062889.t1	MTR_1g011850	60.714	112	42	2	21	131	23	133	3.59e-36	121
MS.gene062889.t1	MTR_1g114050	60.550	109	41	2	21	128	20	127	2.90e-35	119
MS.gene062889.t1	MTR_1g071040	62.500	104	37	2	26	128	28	130	1.54e-34	117
MS.gene062889.t1	MTR_1g069770	48.958	96	48	1	45	139	9	104	5.64e-25	92.4
MS.gene062889.t1	MTR_4g048490	44.762	105	29	2	24	128	26	101	1.70e-16	70.9
MS.gene062889.t1	MTR_7g010440	70.588	51	15	0	78	128	122	172	2.24e-16	74.3
MS.gene062889.t1	MTR_4g005910	74.419	43	11	0	87	129	4	46	3.21e-16	68.6
MS.gene062889.t1	MTR_7g027610	41.525	118	42	4	20	135	10	102	5.62e-15	67.0
MS.gene062889.t1	MTR_0653s0030	54.545	55	25	0	55	109	43	97	4.58e-12	60.5
MS.gene062889.t1	MTR_2g096590	70.000	40	12	0	110	149	12	51	5.34e-12	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062889.t1	AT5G02560	79.739	153	28	1	1	150	1	153	1.48e-77	227
MS.gene062889.t1	AT5G27670	76.316	152	32	3	1	150	1	150	3.32e-74	218
MS.gene062889.t1	AT5G02560	68.927	177	28	2	1	150	1	177	1.06e-72	216
MS.gene062889.t1	AT5G59870	75.000	148	36	1	4	150	3	150	8.98e-72	212
MS.gene062889.t1	AT1G51060	76.667	120	28	0	11	130	3	122	9.15e-61	184
MS.gene062889.t1	AT4G27230	71.538	130	32	1	11	140	3	127	1.43e-59	181
MS.gene062889.t1	AT4G27230	71.538	130	32	1	11	140	3	127	1.43e-59	181
MS.gene062889.t1	AT3G20670	75.630	119	29	0	11	129	3	121	4.77e-59	179
MS.gene062889.t1	AT1G08880	71.654	127	33	1	7	130	2	128	3.47e-58	177
MS.gene062889.t1	AT5G54640	74.790	119	30	0	11	129	3	121	4.51e-58	177
MS.gene062889.t1	AT1G54690	72.868	129	29	3	3	130	5	128	1.19e-57	176
MS.gene062889.t1	AT2G38810	60.714	112	42	2	21	131	25	135	6.97e-37	123
MS.gene062889.t1	AT2G38810	60.714	112	42	2	21	131	25	135	6.97e-37	123
MS.gene062889.t1	AT2G38810	60.714	112	42	2	21	131	25	135	6.97e-37	123
MS.gene062889.t1	AT2G38810	60.714	112	42	2	21	131	25	135	6.97e-37	123
MS.gene062889.t1	AT1G52740	62.857	105	37	2	25	128	27	130	6.18e-35	118
MS.gene062889.t1	AT3G54560	60.185	108	41	2	25	131	29	135	1.25e-34	118
MS.gene062889.t1	AT3G54560	60.185	108	41	2	25	131	29	135	1.25e-34	118
MS.gene062889.t1	AT4G13570	48.913	92	45	2	32	122	28	118	1.97e-20	81.3
MS.gene062889.t1	AT4G13570	48.352	91	45	2	33	122	35	124	5.65e-20	80.1

Find 56 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAGACTTTCCAGCCTTCTT+TGG	0.241789	4.1:-31905824	None:intergenic
AATCAGATCTAAGCCTTCTT+TGG	0.242120	4.1:-31905845	None:intergenic
GTCTTCTTAGGCAACAGAAT+TGG	0.279189	4.1:-31905773	None:intergenic
TTAGCCCTTTCAGTCTTCTT+AGG	0.279305	4.1:-31905785	None:intergenic
CGATTCTTCCGACTGGGAAT+TGG	0.285182	4.1:-31905505	None:intergenic
CTTGTCACCGACTTCTTCCT+TGG	0.303917	4.1:-31905464	None:intergenic
GGCTCCCTTCTTTGTTGTCT+TGG	0.304409	4.1:-31905420	None:intergenic
GAGCATAACGACCTTTCTTC+AGG	0.360921	4.1:-31905535	None:intergenic
GAGCTTGGGAAGTTGCTTGC+TGG	0.378028	4.1:+31905710	MS.gene062889:CDS
TCTTCTTAGGCAACAGAATT+GGG	0.399065	4.1:-31905772	None:intergenic
CATTCCAAGGCATTTGTTGT+TGG	0.411010	4.1:+31905670	MS.gene062889:CDS
GAAGGGAGCCGGTGGAAGAA+AGG	0.417242	4.1:+31905433	MS.gene062889:CDS
AAGGGAGCCGGTGGAAGAAA+GGG	0.429527	4.1:+31905434	MS.gene062889:CDS
CAATTCCCAGTCGGAAGAAT+CGG	0.431861	4.1:+31905506	MS.gene062889:CDS
AACAGAATTGGGTTAATGTT+CGG	0.436254	4.1:-31905761	None:intergenic
AGAACTGCGGCTAGGTAAAC+CGG	0.450323	4.1:-31905578	None:intergenic
TATGCTCAGCGTGTCGGCAC+CGG	0.464760	4.1:+31905551	MS.gene062889:CDS
ACCTCCTCCCTTTCTTCCAC+CGG	0.471747	4.1:-31905441	None:intergenic
CGCTGCTGAAGTTCTTGAGT+TGG	0.474639	4.1:+31905610	MS.gene062889:CDS
GCTGAAGTTCTTGAGTTGGC+TGG	0.476603	4.1:+31905614	MS.gene062889:CDS
GCGTGTCGGCACCGGTGCTC+CGG	0.486643	4.1:+31905559	MS.gene062889:CDS
GAATTGGAGACCGGCTCTGA+TGG	0.486869	4.1:-31905489	None:intergenic
AAGCACCAAGACAACAAAGA+AGG	0.501816	4.1:+31905415	MS.gene062889:CDS
AGCAAGGAACCAAAGAAGGC+TGG	0.510603	4.1:+31905815	MS.gene062889:CDS
GGCTGGAAAGTCTCCAAAGA+AGG	0.525629	4.1:+31905832	MS.gene062889:CDS
ACAGCCAACAACAAATGCCT+TGG	0.530810	4.1:-31905674	None:intergenic
GTGAGGAATGATGAAGAGCT+TGG	0.543487	4.1:+31905695	MS.gene062889:CDS
ATCGGTCGATACCTGAAGAA+AGG	0.548682	4.1:+31905524	MS.gene062889:CDS
TATCGACCGATTCTTCCGAC+TGG	0.549955	4.1:-31905512	None:intergenic
TGGTAGCAAGGAACCAAAGA+AGG	0.550474	4.1:+31905811	MS.gene062889:CDS
TGTTGCCTAAGAAGACTGAA+AGG	0.556495	4.1:+31905780	MS.gene062889:CDS
GGCTAGGTAAACCGGAGCAC+CGG	0.557328	4.1:-31905570	None:intergenic
AGCACCAAGACAACAAAGAA+GGG	0.558080	4.1:+31905416	MS.gene062889:CDS
GAAGAAAGGGAGGAGGTCCA+AGG	0.558674	4.1:+31905447	MS.gene062889:CDS
TGAGGAATGATGAAGAGCTT+GGG	0.560327	4.1:+31905696	MS.gene062889:CDS
TCCGACTGGGAATTGGAGAC+CGG	0.563841	4.1:-31905498	None:intergenic
AAGAACACCACCATGAGCTA+TGG	0.573393	4.1:-31905738	None:intergenic
AAGACAACAAAGAAGGGAGC+CGG	0.578427	4.1:+31905422	MS.gene062889:CDS
GCTGGTGTTACCATAGCTCA+TGG	0.586669	4.1:+31905728	MS.gene062889:CDS
GGTCGTTATGCTCAGCGTGT+CGG	0.588968	4.1:+31905545	MS.gene062889:CDS
GTGACAAGATCCATCAGAGC+CGG	0.589197	4.1:+31905479	MS.gene062889:CDS
GTTGCCTAAGAAGACTGAAA+GGG	0.589268	4.1:+31905781	MS.gene062889:CDS
GCCGGTGGAAGAAAGGGAGG+AGG	0.593863	4.1:+31905440	MS.gene062889:CDS
AAGGGCTAACACTGGTAGCA+AGG	0.594830	4.1:+31905799	MS.gene062889:CDS
ACAACAAAGAAGGGAGCCGG+TGG	0.595449	4.1:+31905425	MS.gene062889:CDS
GGAGCCGGTGGAAGAAAGGG+AGG	0.604037	4.1:+31905437	MS.gene062889:CDS
ATCGACCGATTCTTCCGACT+GGG	0.613102	4.1:-31905511	None:intergenic
AGAAGAATAGAATCATTCCA+AGG	0.617592	4.1:+31905657	MS.gene062889:CDS
AGGAGGTCCAAGGAAGAAGT+CGG	0.620460	4.1:+31905457	MS.gene062889:CDS
GGTATTCAAGAACTGCGGCT+AGG	0.625337	4.1:-31905586	None:intergenic
GGCATTTGTTGTTGGCTGTG+AGG	0.627947	4.1:+31905678	MS.gene062889:CDS
GCCGGTCTCCAATTCCCAGT+CGG	0.630576	4.1:+31905497	MS.gene062889:CDS
AAGACTGAAAGGGCTAACAC+TGG	0.665786	4.1:+31905791	MS.gene062889:CDS
AGCGAGGTATTCAAGAACTG+CGG	0.702914	4.1:-31905591	None:intergenic
ACTCAAGAACTTCAGCAGCG+AGG	0.730395	4.1:-31905607	None:intergenic
GGTGTTACCATAGCTCATGG+TGG	0.742529	4.1:+31905731	MS.gene062889:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AACAGAATTGGGTTAATGTT+CGG	-	chr4.1:31905764-31905783	None:intergenic	30.0%
	AGAAGAATAGAATCATTCCA+AGG	+	chr4.1:31905657-31905676	MS.gene062889:CDS	30.0%
	TCTTCTTAGGCAACAGAATT+GGG	-	chr4.1:31905775-31905794	None:intergenic	35.0%
	AAGCACCAAGACAACAAAGA+AGG	+	chr4.1:31905415-31905434	MS.gene062889:CDS	40.0%
	AGCACCAAGACAACAAAGAA+GGG	+	chr4.1:31905416-31905435	MS.gene062889:CDS	40.0%
	GTCTTCTTAGGCAACAGAAT+TGG	-	chr4.1:31905776-31905795	None:intergenic	40.0%
	GTTGCCTAAGAAGACTGAAA+GGG	+	chr4.1:31905781-31905800	MS.gene062889:CDS	40.0%
	TGAGGAATGATGAAGAGCTT+GGG	+	chr4.1:31905696-31905715	MS.gene062889:CDS	40.0%
	TGTTGCCTAAGAAGACTGAA+AGG	+	chr4.1:31905780-31905799	MS.gene062889:CDS	40.0%
	TTAGCCCTTTCAGTCTTCTT+AGG	-	chr4.1:31905788-31905807	None:intergenic	40.0%
!!	CATTCCAAGGCATTTGTTGT+TGG	+	chr4.1:31905670-31905689	MS.gene062889:CDS	40.0%
	AAGAACACCACCATGAGCTA+TGG	-	chr4.1:31905741-31905760	None:intergenic	45.0%
	AAGACAACAAAGAAGGGAGC+CGG	+	chr4.1:31905422-31905441	MS.gene062889:CDS	45.0%
	AAGACTGAAAGGGCTAACAC+TGG	+	chr4.1:31905791-31905810	MS.gene062889:CDS	45.0%
	ACAGCCAACAACAAATGCCT+TGG	-	chr4.1:31905677-31905696	None:intergenic	45.0%
	ATCGGTCGATACCTGAAGAA+AGG	+	chr4.1:31905524-31905543	MS.gene062889:CDS	45.0%
	CAATTCCCAGTCGGAAGAAT+CGG	+	chr4.1:31905506-31905525	MS.gene062889:CDS	45.0%
	GAGCATAACGACCTTTCTTC+AGG	-	chr4.1:31905538-31905557	None:intergenic	45.0%
	GTGAGGAATGATGAAGAGCT+TGG	+	chr4.1:31905695-31905714	MS.gene062889:CDS	45.0%
	TGGTAGCAAGGAACCAAAGA+AGG	+	chr4.1:31905811-31905830	MS.gene062889:CDS	45.0%
!	AGCGAGGTATTCAAGAACTG+CGG	-	chr4.1:31905594-31905613	None:intergenic	45.0%
	AAGGGCTAACACTGGTAGCA+AGG	+	chr4.1:31905799-31905818	MS.gene062889:CDS	50.0%
	ACTCAAGAACTTCAGCAGCG+AGG	-	chr4.1:31905610-31905629	None:intergenic	50.0%
	AGAACTGCGGCTAGGTAAAC+CGG	-	chr4.1:31905581-31905600	None:intergenic	50.0%
	AGCAAGGAACCAAAGAAGGC+TGG	+	chr4.1:31905815-31905834	MS.gene062889:CDS	50.0%
	AGGAGGTCCAAGGAAGAAGT+CGG	+	chr4.1:31905457-31905476	MS.gene062889:CDS	50.0%
	CGATTCTTCCGACTGGGAAT+TGG	-	chr4.1:31905508-31905527	None:intergenic	50.0%
	CGCTGCTGAAGTTCTTGAGT+TGG	+	chr4.1:31905610-31905629	MS.gene062889:CDS	50.0%
	GGAGACTTTCCAGCCTTCTT+TGG	-	chr4.1:31905827-31905846	None:intergenic	50.0%
	GGCTGGAAAGTCTCCAAAGA+AGG	+	chr4.1:31905832-31905851	MS.gene062889:CDS	50.0%
	GGTATTCAAGAACTGCGGCT+AGG	-	chr4.1:31905589-31905608	None:intergenic	50.0%
	GTGACAAGATCCATCAGAGC+CGG	+	chr4.1:31905479-31905498	MS.gene062889:CDS	50.0%
!	ATCGACCGATTCTTCCGACT+GGG	-	chr4.1:31905514-31905533	None:intergenic	50.0%
!	GGCTCCCTTCTTTGTTGTCT+TGG	-	chr4.1:31905423-31905442	None:intergenic	50.0%
!	GGTGTTACCATAGCTCATGG+TGG	+	chr4.1:31905731-31905750	MS.gene062889:CDS	50.0%
!	TATCGACCGATTCTTCCGAC+TGG	-	chr4.1:31905515-31905534	None:intergenic	50.0%
!!	CTTGTCACCGACTTCTTCCT+TGG	-	chr4.1:31905467-31905486	None:intergenic	50.0%
!!	GCTGAAGTTCTTGAGTTGGC+TGG	+	chr4.1:31905614-31905633	MS.gene062889:CDS	50.0%
!!	GCTGGTGTTACCATAGCTCA+TGG	+	chr4.1:31905728-31905747	MS.gene062889:CDS	50.0%
!!	GGCATTTGTTGTTGGCTGTG+AGG	+	chr4.1:31905678-31905697	MS.gene062889:CDS	50.0%
	AAGGGAGCCGGTGGAAGAAA+GGG	+	chr4.1:31905434-31905453	MS.gene062889:CDS	55.0%
	ACAACAAAGAAGGGAGCCGG+TGG	+	chr4.1:31905425-31905444	MS.gene062889:CDS	55.0%
	ACCTCCTCCCTTTCTTCCAC+CGG	-	chr4.1:31905444-31905463	None:intergenic	55.0%
	GAAGAAAGGGAGGAGGTCCA+AGG	+	chr4.1:31905447-31905466	MS.gene062889:CDS	55.0%
	GAGCTTGGGAAGTTGCTTGC+TGG	+	chr4.1:31905710-31905729	MS.gene062889:CDS	55.0%
	GGTCGTTATGCTCAGCGTGT+CGG	+	chr4.1:31905545-31905564	MS.gene062889:CDS	55.0%
	TCCGACTGGGAATTGGAGAC+CGG	-	chr4.1:31905501-31905520	None:intergenic	55.0%
!	GAATTGGAGACCGGCTCTGA+TGG	-	chr4.1:31905492-31905511	None:intergenic	55.0%
	GAAGGGAGCCGGTGGAAGAA+AGG	+	chr4.1:31905433-31905452	MS.gene062889:CDS	60.0%
	GCCGGTCTCCAATTCCCAGT+CGG	+	chr4.1:31905497-31905516	MS.gene062889:CDS	60.0%
	GGCTAGGTAAACCGGAGCAC+CGG	-	chr4.1:31905573-31905592	None:intergenic	60.0%
!	TATGCTCAGCGTGTCGGCAC+CGG	+	chr4.1:31905551-31905570	MS.gene062889:CDS	60.0%
	GCCGGTGGAAGAAAGGGAGG+AGG	+	chr4.1:31905440-31905459	MS.gene062889:CDS	65.0%
	GGAGCCGGTGGAAGAAAGGG+AGG	+	chr4.1:31905437-31905456	MS.gene062889:CDS	65.0%
!!	GCGTGTCGGCACCGGTGCTC+CGG	+	chr4.1:31905559-31905578	MS.gene062889:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	31905407	31905859	31905407	ID=MS.gene062889
chr4.1	mRNA	31905407	31905859	31905407	ID=MS.gene062889.t1;Parent=MS.gene062889
chr4.1	exon	31905407	31905859	31905407	ID=MS.gene062889.t1.exon1;Parent=MS.gene062889.t1
chr4.1	CDS	31905407	31905859	31905407	ID=cds.MS.gene062889.t1;Parent=MS.gene062889.t1

Gene Sequence

>MS.gene062889

ATGGACGCAAGCACCAAGACAACAAAGAAGGGAGCCGGTGGAAGAAAGGGAGGAGGTCCAAGGAAGAAGTCGGTGACAAGATCCATCAGAGCCGGTCTCCAATTCCCAGTCGGAAGAATCGGTCGATACCTGAAGAAAGGTCGTTATGCTCAGCGTGTCGGCACCGGTGCTCCGGTTTACCTAGCCGCAGTTCTTGAATACCTCGCTGCTGAAGTTCTTGAGTTGGCTGGAAACGCTGCTCGTGATAACAAGAAGAATAGAATCATTCCAAGGCATTTGTTGTTGGCTGTGAGGAATGATGAAGAGCTTGGGAAGTTGCTTGCTGGTGTTACCATAGCTCATGGTGGTGTTCTTCCGAACATTAACCCAATTCTGTTGCCTAAGAAGACTGAAAGGGCTAACACTGGTAGCAAGGAACCAAAGAAGGCTGGAAAGTCTCCAAAGAAGGCTTAG

Protein sequence

>MS.gene062889.t1

MDASTKTTKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHLLLAVRNDEELGKLLAGVTIAHGGVLPNINPILLPKKTERANTGSKEPKKAGKSPKKA