AlfalfaGEDB | MS.gene66433

M. sativa cultivar XinJiangDaYe / MS.gene66433

KEGG: K11251
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF00125.27 PF16211.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66433.t1	XP_003607007.1	98	152	1	1	1	150	1	152	8.30E-72	279.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66433.t1	Q1S053	98.0	152	1	1	1	150	1	152	2.0e-74	279.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66433.t1	G7JIM1	98.0	152	1	1	1	150	1	152	6.0e-72	279.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049705	MS.gene66433	0.815744	7.90E-52	-1.69E-46
MS.gene04972	MS.gene66433	0.811821	5.78E-51	-1.69E-46
MS.gene049989	MS.gene66433	0.802422	5.65E-49	-1.69E-46
MS.gene050297	MS.gene66433	0.80674	7.10E-50	-1.69E-46
MS.gene051263	MS.gene66433	0.857755	1.31E-62	-1.69E-46
MS.gene055957	MS.gene66433	0.862265	5.73E-64	-1.69E-46
MS.gene056041	MS.gene66433	0.800395	1.47E-48	-1.69E-46
MS.gene056335	MS.gene66433	0.816507	5.34E-52	-1.69E-46
MS.gene057458	MS.gene66433	0.806823	6.82E-50	-1.69E-46
MS.gene057632	MS.gene66433	0.817585	3.05E-52	-1.69E-46
MS.gene058844	MS.gene66433	0.808619	2.83E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66433.t1	MTR_4g071150	98.026	152	1	1	1	150	1	152	9.78e-101	285
MS.gene66433.t1	MTR_2g096610	87.333	150	17	1	1	150	1	148	4.41e-83	241
MS.gene66433.t1	MTR_4g064005	94.000	150	7	1	1	150	1	148	1.32e-82	239
MS.gene66433.t1	MTR_4g063410	94.000	150	7	1	1	150	1	148	2.70e-82	239
MS.gene66433.t1	MTR_2g096570	91.558	154	8	2	1	150	1	153	5.64e-82	238
MS.gene66433.t1	MTR_4g063280	92.424	132	9	1	20	150	14	145	2.84e-81	236
MS.gene66433.t1	MTR_4g064967	93.333	150	8	1	1	150	1	148	2.98e-80	234
MS.gene66433.t1	MTR_8g086640	96.212	132	5	0	1	132	1	132	4.27e-75	221
MS.gene66433.t1	MTR_7g113160	79.699	133	26	1	10	142	13	144	5.01e-71	210
MS.gene66433.t1	MTR_7g108320	75.000	128	26	3	4	130	6	128	7.58e-60	182
MS.gene66433.t1	MTR_7g114060	74.219	128	29	1	3	130	2	125	7.85e-60	182
MS.gene66433.t1	MTR_7g114040	74.219	128	29	1	3	130	2	125	7.85e-60	182
MS.gene66433.t1	MTR_7g093910	73.228	127	31	1	4	130	5	128	9.33e-60	181
MS.gene66433.t1	MTR_2g082510	70.543	129	38	0	11	139	3	131	3.21e-59	180
MS.gene66433.t1	MTR_2g082220	73.950	119	31	0	11	129	3	121	1.65e-57	176
MS.gene66433.t1	MTR_6g005150	62.992	127	33	3	24	149	26	139	3.16e-47	150
MS.gene66433.t1	MTR_4g063300	75.238	105	21	1	45	149	10	109	3.34e-46	146
MS.gene66433.t1	MTR_7g114050	68.644	118	22	3	17	130	10	116	2.19e-45	145
MS.gene66433.t1	MTR_1g011750	60.714	112	42	2	21	131	23	133	3.59e-36	121
MS.gene66433.t1	MTR_1g011850	60.714	112	42	2	21	131	23	133	3.59e-36	121
MS.gene66433.t1	MTR_1g114050	60.550	109	41	2	21	128	20	127	2.90e-35	119
MS.gene66433.t1	MTR_1g071040	62.500	104	37	2	26	128	28	130	1.54e-34	117
MS.gene66433.t1	MTR_1g069770	48.958	96	48	1	45	139	9	104	5.64e-25	92.4
MS.gene66433.t1	MTR_4g048490	44.762	105	29	2	24	128	26	101	1.70e-16	70.9
MS.gene66433.t1	MTR_7g010440	70.588	51	15	0	78	128	122	172	2.24e-16	74.3
MS.gene66433.t1	MTR_4g005910	74.419	43	11	0	87	129	4	46	3.21e-16	68.6
MS.gene66433.t1	MTR_7g027610	41.525	118	42	4	20	135	10	102	5.62e-15	67.0
MS.gene66433.t1	MTR_0653s0030	54.545	55	25	0	55	109	43	97	4.58e-12	60.5
MS.gene66433.t1	MTR_2g096590	70.000	40	12	0	110	149	12	51	5.34e-12	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66433.t1	AT5G02560	79.739	153	28	1	1	150	1	153	1.48e-77	227
MS.gene66433.t1	AT5G27670	76.316	152	32	3	1	150	1	150	3.32e-74	218
MS.gene66433.t1	AT5G02560	68.927	177	28	2	1	150	1	177	1.06e-72	216
MS.gene66433.t1	AT5G59870	75.000	148	36	1	4	150	3	150	8.98e-72	212
MS.gene66433.t1	AT1G51060	76.667	120	28	0	11	130	3	122	9.15e-61	184
MS.gene66433.t1	AT4G27230	71.538	130	32	1	11	140	3	127	1.43e-59	181
MS.gene66433.t1	AT4G27230	71.538	130	32	1	11	140	3	127	1.43e-59	181
MS.gene66433.t1	AT3G20670	75.630	119	29	0	11	129	3	121	4.77e-59	179
MS.gene66433.t1	AT1G08880	71.654	127	33	1	7	130	2	128	3.47e-58	177
MS.gene66433.t1	AT5G54640	74.790	119	30	0	11	129	3	121	4.51e-58	177
MS.gene66433.t1	AT1G54690	72.868	129	29	3	3	130	5	128	1.19e-57	176
MS.gene66433.t1	AT2G38810	60.714	112	42	2	21	131	25	135	6.97e-37	123
MS.gene66433.t1	AT2G38810	60.714	112	42	2	21	131	25	135	6.97e-37	123
MS.gene66433.t1	AT2G38810	60.714	112	42	2	21	131	25	135	6.97e-37	123
MS.gene66433.t1	AT2G38810	60.714	112	42	2	21	131	25	135	6.97e-37	123
MS.gene66433.t1	AT1G52740	62.857	105	37	2	25	128	27	130	6.18e-35	118
MS.gene66433.t1	AT3G54560	60.185	108	41	2	25	131	29	135	1.25e-34	118
MS.gene66433.t1	AT3G54560	60.185	108	41	2	25	131	29	135	1.25e-34	118
MS.gene66433.t1	AT4G13570	48.913	92	45	2	32	122	28	118	1.97e-20	81.3
MS.gene66433.t1	AT4G13570	48.352	91	45	2	33	122	35	124	5.65e-20	80.1

Find 56 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAGACTTTCCAGCCTTCTT+TGG	0.241789	4.1:+77061921	None:intergenic
AATCAGATCTAAGCCTTCTT+TGG	0.242120	4.1:+77061900	None:intergenic
GTCTTCTTAGGCAACAGAAT+TGG	0.279189	4.1:+77061972	None:intergenic
TTAGCCCTTTCAGTCTTCTT+AGG	0.279305	4.1:+77061960	None:intergenic
CGATTCTTCCGACTGGGAAT+TGG	0.285182	4.1:+77062240	None:intergenic
CTTGTCACCGACTTCTTCCT+TGG	0.303917	4.1:+77062281	None:intergenic
GGCTCCCTTCTTTGTTGTCT+TGG	0.304409	4.1:+77062325	None:intergenic
GAGCATAACGACCTTTCTTC+AGG	0.360921	4.1:+77062210	None:intergenic
GAGCTTGGGAAGTTGCTTGC+TGG	0.378028	4.1:-77062035	MS.gene66433:CDS
TCTTCTTAGGCAACAGAATT+GGG	0.399065	4.1:+77061973	None:intergenic
CATTCCAAGGCATTTGTTGT+TGG	0.411010	4.1:-77062075	MS.gene66433:CDS
GAAGGGAGCCGGTGGAAGAA+AGG	0.417242	4.1:-77062312	MS.gene66433:CDS
AAGGGAGCCGGTGGAAGAAA+GGG	0.429527	4.1:-77062311	MS.gene66433:CDS
CAATTCCCAGTCGGAAGAAT+CGG	0.431861	4.1:-77062239	MS.gene66433:CDS
AACAGAATTGGGTTAATGTT+CGG	0.436254	4.1:+77061984	None:intergenic
AGAACTGCGGCTAGGTAAAC+CGG	0.450323	4.1:+77062167	None:intergenic
TATGCTCAGCGTGTCGGCAC+CGG	0.464760	4.1:-77062194	MS.gene66433:CDS
ACCTCCTCCCTTTCTTCCAC+CGG	0.471747	4.1:+77062304	None:intergenic
CGCTGCTGAAGTTCTTGAGT+TGG	0.474639	4.1:-77062135	MS.gene66433:CDS
GCTGAAGTTCTTGAGTTGGC+TGG	0.476603	4.1:-77062131	MS.gene66433:CDS
GCGTGTCGGCACCGGTGCTC+CGG	0.486643	4.1:-77062186	MS.gene66433:CDS
GAATTGGAGACCGGCTCTGA+TGG	0.486869	4.1:+77062256	None:intergenic
AAGCACCAAGACAACAAAGA+AGG	0.501816	4.1:-77062330	MS.gene66433:CDS
AGCAAGGAACCAAAGAAGGC+TGG	0.510603	4.1:-77061930	MS.gene66433:CDS
GGCTGGAAAGTCTCCAAAGA+AGG	0.525629	4.1:-77061913	MS.gene66433:CDS
ACAGCCAACAACAAATGCCT+TGG	0.530810	4.1:+77062071	None:intergenic
GTGAGGAATGATGAAGAGCT+TGG	0.543487	4.1:-77062050	MS.gene66433:CDS
ATCGGTCGATACCTGAAGAA+AGG	0.548682	4.1:-77062221	MS.gene66433:CDS
TATCGACCGATTCTTCCGAC+TGG	0.549955	4.1:+77062233	None:intergenic
TGGTAGCAAGGAACCAAAGA+AGG	0.550474	4.1:-77061934	MS.gene66433:CDS
TGTTGCCTAAGAAGACTGAA+AGG	0.556495	4.1:-77061965	MS.gene66433:CDS
GGCTAGGTAAACCGGAGCAC+CGG	0.557328	4.1:+77062175	None:intergenic
AGCACCAAGACAACAAAGAA+GGG	0.558080	4.1:-77062329	MS.gene66433:CDS
GAAGAAAGGGAGGAGGTCCA+AGG	0.558674	4.1:-77062298	MS.gene66433:CDS
TGAGGAATGATGAAGAGCTT+GGG	0.560327	4.1:-77062049	MS.gene66433:CDS
TCCGACTGGGAATTGGAGAC+CGG	0.563841	4.1:+77062247	None:intergenic
AAGAACACCACCATGAGCTA+TGG	0.573393	4.1:+77062007	None:intergenic
AAGACAACAAAGAAGGGAGC+CGG	0.578427	4.1:-77062323	MS.gene66433:CDS
GCTGGTGTTACCATAGCTCA+TGG	0.586669	4.1:-77062017	MS.gene66433:CDS
GGTCGTTATGCTCAGCGTGT+CGG	0.588968	4.1:-77062200	MS.gene66433:CDS
GTGACAAGATCCATCAGAGC+CGG	0.589197	4.1:-77062266	MS.gene66433:CDS
GTTGCCTAAGAAGACTGAAA+GGG	0.589268	4.1:-77061964	MS.gene66433:CDS
GCCGGTGGAAGAAAGGGAGG+AGG	0.593863	4.1:-77062305	MS.gene66433:CDS
AAGGGCTAACACTGGTAGCA+AGG	0.594830	4.1:-77061946	MS.gene66433:CDS
ACAACAAAGAAGGGAGCCGG+TGG	0.595449	4.1:-77062320	MS.gene66433:CDS
GGAGCCGGTGGAAGAAAGGG+AGG	0.604037	4.1:-77062308	MS.gene66433:CDS
ATCGACCGATTCTTCCGACT+GGG	0.613102	4.1:+77062234	None:intergenic
AGAAGAATAGAATCATTCCA+AGG	0.617592	4.1:-77062088	MS.gene66433:CDS
AGGAGGTCCAAGGAAGAAGT+CGG	0.620460	4.1:-77062288	MS.gene66433:CDS
GGTATTCAAGAACTGCGGCT+AGG	0.625337	4.1:+77062159	None:intergenic
GGCATTTGTTGTTGGCTGTG+AGG	0.627947	4.1:-77062067	MS.gene66433:CDS
GCCGGTCTCCAATTCCCAGT+CGG	0.630576	4.1:-77062248	MS.gene66433:CDS
AAGACTGAAAGGGCTAACAC+TGG	0.665786	4.1:-77061954	MS.gene66433:CDS
AGCGAGGTATTCAAGAACTG+CGG	0.702914	4.1:+77062154	None:intergenic
ACTCAAGAACTTCAGCAGCG+AGG	0.730395	4.1:+77062138	None:intergenic
GGTGTTACCATAGCTCATGG+TGG	0.742529	4.1:-77062014	MS.gene66433:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AACAGAATTGGGTTAATGTT+CGG	+	chr4.1:77062265-77062284	None:intergenic	30.0%
	AGAAGAATAGAATCATTCCA+AGG	-	chr4.1:77062158-77062177	MS.gene66433:CDS	30.0%
	TCTTCTTAGGCAACAGAATT+GGG	+	chr4.1:77062276-77062295	None:intergenic	35.0%
	AAGCACCAAGACAACAAAGA+AGG	-	chr4.1:77061916-77061935	MS.gene66433:CDS	40.0%
	AGCACCAAGACAACAAAGAA+GGG	-	chr4.1:77061917-77061936	MS.gene66433:CDS	40.0%
	GTCTTCTTAGGCAACAGAAT+TGG	+	chr4.1:77062277-77062296	None:intergenic	40.0%
	GTTGCCTAAGAAGACTGAAA+GGG	-	chr4.1:77062282-77062301	MS.gene66433:CDS	40.0%
	TGAGGAATGATGAAGAGCTT+GGG	-	chr4.1:77062197-77062216	MS.gene66433:CDS	40.0%
	TGTTGCCTAAGAAGACTGAA+AGG	-	chr4.1:77062281-77062300	MS.gene66433:CDS	40.0%
	TTAGCCCTTTCAGTCTTCTT+AGG	+	chr4.1:77062289-77062308	None:intergenic	40.0%
!!	CATTCCAAGGCATTTGTTGT+TGG	-	chr4.1:77062171-77062190	MS.gene66433:CDS	40.0%
	AAGAACACCACCATGAGCTA+TGG	+	chr4.1:77062242-77062261	None:intergenic	45.0%
	AAGACAACAAAGAAGGGAGC+CGG	-	chr4.1:77061923-77061942	MS.gene66433:CDS	45.0%
	AAGACTGAAAGGGCTAACAC+TGG	-	chr4.1:77062292-77062311	MS.gene66433:CDS	45.0%
	ACAGCCAACAACAAATGCCT+TGG	+	chr4.1:77062178-77062197	None:intergenic	45.0%
	ATCGGTCGATACCTGAAGAA+AGG	-	chr4.1:77062025-77062044	MS.gene66433:CDS	45.0%
	CAATTCCCAGTCGGAAGAAT+CGG	-	chr4.1:77062007-77062026	MS.gene66433:CDS	45.0%
	GAGCATAACGACCTTTCTTC+AGG	+	chr4.1:77062039-77062058	None:intergenic	45.0%
	GTGAGGAATGATGAAGAGCT+TGG	-	chr4.1:77062196-77062215	MS.gene66433:CDS	45.0%
	TGGTAGCAAGGAACCAAAGA+AGG	-	chr4.1:77062312-77062331	MS.gene66433:CDS	45.0%
!	AGCGAGGTATTCAAGAACTG+CGG	+	chr4.1:77062095-77062114	None:intergenic	45.0%
	AAGGGCTAACACTGGTAGCA+AGG	-	chr4.1:77062300-77062319	MS.gene66433:CDS	50.0%
	ACTCAAGAACTTCAGCAGCG+AGG	+	chr4.1:77062111-77062130	None:intergenic	50.0%
	AGAACTGCGGCTAGGTAAAC+CGG	+	chr4.1:77062082-77062101	None:intergenic	50.0%
	AGCAAGGAACCAAAGAAGGC+TGG	-	chr4.1:77062316-77062335	MS.gene66433:CDS	50.0%
	AGGAGGTCCAAGGAAGAAGT+CGG	-	chr4.1:77061958-77061977	MS.gene66433:CDS	50.0%
	CGATTCTTCCGACTGGGAAT+TGG	+	chr4.1:77062009-77062028	None:intergenic	50.0%
	CGCTGCTGAAGTTCTTGAGT+TGG	-	chr4.1:77062111-77062130	MS.gene66433:CDS	50.0%
	GGAGACTTTCCAGCCTTCTT+TGG	+	chr4.1:77062328-77062347	None:intergenic	50.0%
	GGCTGGAAAGTCTCCAAAGA+AGG	-	chr4.1:77062333-77062352	MS.gene66433:CDS	50.0%
	GGTATTCAAGAACTGCGGCT+AGG	+	chr4.1:77062090-77062109	None:intergenic	50.0%
	GTGACAAGATCCATCAGAGC+CGG	-	chr4.1:77061980-77061999	MS.gene66433:CDS	50.0%
!	ATCGACCGATTCTTCCGACT+GGG	+	chr4.1:77062015-77062034	None:intergenic	50.0%
!	GGCTCCCTTCTTTGTTGTCT+TGG	+	chr4.1:77061924-77061943	None:intergenic	50.0%
!	GGTGTTACCATAGCTCATGG+TGG	-	chr4.1:77062232-77062251	MS.gene66433:CDS	50.0%
!	TATCGACCGATTCTTCCGAC+TGG	+	chr4.1:77062016-77062035	None:intergenic	50.0%
!!	CTTGTCACCGACTTCTTCCT+TGG	+	chr4.1:77061968-77061987	None:intergenic	50.0%
!!	GCTGAAGTTCTTGAGTTGGC+TGG	-	chr4.1:77062115-77062134	MS.gene66433:CDS	50.0%
!!	GCTGGTGTTACCATAGCTCA+TGG	-	chr4.1:77062229-77062248	MS.gene66433:CDS	50.0%
!!	GGCATTTGTTGTTGGCTGTG+AGG	-	chr4.1:77062179-77062198	MS.gene66433:CDS	50.0%
	AAGGGAGCCGGTGGAAGAAA+GGG	-	chr4.1:77061935-77061954	MS.gene66433:CDS	55.0%
	ACAACAAAGAAGGGAGCCGG+TGG	-	chr4.1:77061926-77061945	MS.gene66433:CDS	55.0%
	ACCTCCTCCCTTTCTTCCAC+CGG	+	chr4.1:77061945-77061964	None:intergenic	55.0%
	GAAGAAAGGGAGGAGGTCCA+AGG	-	chr4.1:77061948-77061967	MS.gene66433:CDS	55.0%
	GAGCTTGGGAAGTTGCTTGC+TGG	-	chr4.1:77062211-77062230	MS.gene66433:CDS	55.0%
	GGTCGTTATGCTCAGCGTGT+CGG	-	chr4.1:77062046-77062065	MS.gene66433:CDS	55.0%
	TCCGACTGGGAATTGGAGAC+CGG	+	chr4.1:77062002-77062021	None:intergenic	55.0%
!	GAATTGGAGACCGGCTCTGA+TGG	+	chr4.1:77061993-77062012	None:intergenic	55.0%
	GAAGGGAGCCGGTGGAAGAA+AGG	-	chr4.1:77061934-77061953	MS.gene66433:CDS	60.0%
	GCCGGTCTCCAATTCCCAGT+CGG	-	chr4.1:77061998-77062017	MS.gene66433:CDS	60.0%
	GGCTAGGTAAACCGGAGCAC+CGG	+	chr4.1:77062074-77062093	None:intergenic	60.0%
!	TATGCTCAGCGTGTCGGCAC+CGG	-	chr4.1:77062052-77062071	MS.gene66433:CDS	60.0%
	GCCGGTGGAAGAAAGGGAGG+AGG	-	chr4.1:77061941-77061960	MS.gene66433:CDS	65.0%
	GGAGCCGGTGGAAGAAAGGG+AGG	-	chr4.1:77061938-77061957	MS.gene66433:CDS	65.0%
!!	GCGTGTCGGCACCGGTGCTC+CGG	-	chr4.1:77062060-77062079	MS.gene66433:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	77061908	77062360	77061908	ID=MS.gene66433
chr4.1	mRNA	77061908	77062360	77061908	ID=MS.gene66433.t1;Parent=MS.gene66433
chr4.1	exon	77061908	77062360	77061908	ID=MS.gene66433.t1.exon1;Parent=MS.gene66433.t1
chr4.1	CDS	77061908	77062360	77061908	ID=cds.MS.gene66433.t1;Parent=MS.gene66433.t1

Gene Sequence

>MS.gene66433

ATGGACGCAAGCACCAAGACAACAAAGAAGGGAGCCGGTGGAAGAAAGGGAGGAGGTCCAAGGAAGAAGTCGGTGACAAGATCCATCAGAGCCGGTCTCCAATTCCCAGTCGGAAGAATCGGTCGATACCTGAAGAAAGGTCGTTATGCTCAGCGTGTCGGCACCGGTGCTCCGGTTTACCTAGCCGCAGTTCTTGAATACCTCGCTGCTGAAGTTCTTGAGTTGGCTGGAAACGCTGCTCGTGATAACAAGAAGAATAGAATCATTCCAAGGCATTTGTTGTTGGCTGTGAGGAATGATGAAGAGCTTGGGAAGTTGCTTGCTGGTGTTACCATAGCTCATGGTGGTGTTCTTCCGAACATTAACCCAATTCTGTTGCCTAAGAAGACTGAAAGGGCTAACACTGGTAGCAAGGAACCAAAGAAGGCTGGAAAGTCTCCAAAGAAGGCTTAG

Protein sequence

>MS.gene66433.t1

MDASTKTTKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHLLLAVRNDEELGKLLAGVTIAHGGVLPNINPILLPKKTERANTGSKEPKKAGKSPKKA