AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057701

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057701.t1 MTR_4g073720 87.931 348 7 2 1 317 1 344 0.0 593
MS.gene057701.t1 MTR_5g022350 55.949 311 123 4 8 317 14 311 7.90e-119 345
MS.gene057701.t1 MTR_8g075510 70.213 235 64 3 14 245 18 249 2.10e-116 336
MS.gene057701.t1 MTR_8g075510 63.333 270 93 3 14 280 18 284 2.24e-115 335
MS.gene057701.t1 MTR_1g025420 61.977 263 88 6 8 258 3 265 4.73e-105 310
MS.gene057701.t1 MTR_1g025420 63.600 250 79 6 8 246 3 251 8.92e-104 305
MS.gene057701.t1 MTR_8g037890 57.588 257 101 5 9 257 9 265 2.56e-97 290
MS.gene057701.t1 MTR_8g075550 61.506 239 80 4 20 247 23 260 1.18e-95 285
MS.gene057701.t1 MTR_3g114030 63.478 230 76 5 21 242 3 232 9.55e-95 280
MS.gene057701.t1 MTR_5g022310 54.275 269 108 5 9 266 14 278 2.65e-92 276
MS.gene057701.t1 MTR_5g022310 56.855 248 92 5 9 246 14 256 9.82e-92 273
MS.gene057701.t1 MTR_8g056820 56.627 249 95 7 9 246 9 255 2.96e-89 267
MS.gene057701.t1 MTR_4g073730 54.878 246 101 4 3 240 5 248 3.65e-89 268
MS.gene057701.t1 MTR_4g073730 54.878 246 101 4 3 240 5 248 7.54e-89 269
MS.gene057701.t1 MTR_6g079580 53.586 237 103 4 9 240 6 240 6.13e-88 263
MS.gene057701.t1 MTR_3g111620 55.263 228 94 5 23 244 25 250 1.72e-84 255
MS.gene057701.t1 MTR_8g036215 54.077 233 102 3 11 239 6 237 4.62e-84 253
MS.gene057701.t1 MTR_1g021945 56.637 226 90 4 25 244 26 249 2.22e-83 252
MS.gene057701.t1 MTR_8g107140 51.807 249 109 6 3 242 1 247 2.57e-82 249
MS.gene057701.t1 MTR_7g102380 50.206 243 114 5 6 242 2 243 6.58e-80 243
MS.gene057701.t1 MTR_1g062590 52.675 243 108 5 4 240 1 242 3.95e-79 241
MS.gene057701.t1 MTR_1g062630 54.626 227 96 5 22 242 19 244 7.54e-79 241
MS.gene057701.t1 MTR_1g062660 51.837 245 111 5 4 242 1 244 2.83e-76 234
MS.gene057701.t1 MTR_2g069660 51.055 237 108 4 11 240 8 243 4.57e-76 233
MS.gene057701.t1 MTR_1g062370 51.429 245 112 5 4 242 1 244 7.95e-76 233
MS.gene057701.t1 MTR_1g062350 51.429 245 112 5 4 242 1 244 8.58e-76 233
MS.gene057701.t1 MTR_1g062340 54.185 227 97 5 22 242 19 244 7.86e-75 230
MS.gene057701.t1 MTR_5g059200 46.617 266 129 8 9 269 11 268 4.62e-73 227
MS.gene057701.t1 MTR_2g068655 49.351 231 111 4 21 245 27 257 1.61e-70 221
MS.gene057701.t1 MTR_2g068030 49.772 219 104 4 27 239 25 243 3.03e-70 219
MS.gene057701.t1 MTR_2g067980 50.218 229 106 4 22 242 21 249 1.22e-68 231
MS.gene057701.t1 MTR_3g068015 45.982 224 114 4 23 240 26 248 2.23e-68 214
MS.gene057701.t1 MTR_7g062610 45.798 238 119 4 8 240 10 242 3.82e-67 211
MS.gene057701.t1 MTR_6g009480 43.701 254 129 8 1 241 1 253 1.28e-64 205
MS.gene057701.t1 MTR_4g063630 45.417 240 117 8 10 239 16 251 2.88e-63 201
MS.gene057701.t1 MTR_5g022350 67.123 146 43 2 11 154 80 222 2.36e-61 195
MS.gene057701.t1 MTR_1g062690 44.672 244 106 5 4 242 1 220 1.28e-59 190
MS.gene057701.t1 MTR_5g010635 46.575 219 97 10 25 240 24 225 5.28e-50 166
MS.gene057701.t1 MTR_5g023850 40.234 256 100 10 4 246 7 222 3.02e-48 161
MS.gene057701.t1 MTR_8g096900 41.880 234 113 10 11 240 34 248 1.11e-47 160
MS.gene057701.t1 MTR_5g010640 38.800 250 129 10 1 240 1 236 2.91e-45 154
MS.gene057701.t1 MTR_2g067990 42.291 227 101 8 21 242 23 224 1.09e-44 163
MS.gene057701.t1 MTR_8g089020 47.134 157 59 3 79 232 21 156 1.24e-41 142
MS.gene057701.t1 MTR_2g067970 41.270 189 102 5 67 247 2 189 7.53e-40 149
MS.gene057701.t1 MTR_7g076360 36.893 206 72 3 25 230 8 155 9.93e-38 132
MS.gene057701.t1 MTR_2g063150 31.148 244 149 7 3 232 4 242 3.78e-35 128
MS.gene057701.t1 MTR_1g062390 30.769 247 149 7 6 241 4 239 3.46e-31 117
MS.gene057701.t1 MTR_8g096910 44.304 158 73 8 9 164 6 150 6.37e-27 104
MS.gene057701.t1 MTR_8g096920 46.617 133 57 7 9 139 6 126 3.63e-26 102
MS.gene057701.t1 MTR_1g062640 39.130 115 68 2 51 164 33 146 6.79e-18 79.7
MS.gene057701.t1 MTR_3g081550 50.526 95 40 4 109 196 15 109 2.23e-16 74.3
MS.gene057701.t1 MTR_5g068670 48.864 88 34 4 62 141 5 89 2.63e-14 69.3
MS.gene057701.t1 MTR_8g088960 45.946 74 36 3 125 196 14 85 4.89e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057701.t1 AT4G38660 65.385 338 87 7 5 314 9 344 8.93e-144 409
MS.gene057701.t1 AT4G38660 66.460 322 79 6 20 314 3 322 1.72e-140 400
MS.gene057701.t1 AT4G24180 68.254 252 70 4 5 248 10 259 2.49e-120 348
MS.gene057701.t1 AT4G24180 67.843 255 71 4 5 248 10 264 5.62e-120 347
MS.gene057701.t1 AT4G24180 68.421 247 68 4 5 243 10 254 2.44e-118 341
MS.gene057701.t1 AT4G24180 68.000 250 69 4 5 243 10 259 4.30e-118 341
MS.gene057701.t1 AT4G24180 66.812 229 66 4 5 225 10 236 6.04e-106 312
MS.gene057701.t1 AT4G24180 65.948 232 68 4 5 225 10 241 1.02e-105 312
MS.gene057701.t1 AT1G75800 58.077 260 96 6 1 249 1 258 2.06e-96 288
MS.gene057701.t1 AT1G20030 64.502 231 72 5 21 241 16 246 4.11e-96 287
MS.gene057701.t1 AT1G20030 65.487 226 68 5 26 241 4 229 3.09e-95 284
MS.gene057701.t1 AT4G36010 58.468 248 90 5 21 255 19 266 4.71e-93 278
MS.gene057701.t1 AT4G36010 58.468 248 90 5 21 255 19 266 4.71e-93 278
MS.gene057701.t1 AT1G19320 56.855 248 96 5 1 239 1 246 2.62e-92 275
MS.gene057701.t1 AT1G75030 56.452 248 97 6 1 241 1 244 8.72e-90 268
MS.gene057701.t1 AT2G17860 54.297 256 103 6 1 245 1 253 4.09e-88 264
MS.gene057701.t1 AT1G75040 56.557 244 97 6 1 240 1 239 4.09e-87 261
MS.gene057701.t1 AT1G75050 56.379 243 96 6 6 241 5 244 1.37e-86 260
MS.gene057701.t1 AT1G18250 56.000 225 92 4 23 242 19 241 2.23e-85 257
MS.gene057701.t1 AT1G18250 56.502 223 90 4 25 242 22 242 2.65e-85 257
MS.gene057701.t1 AT1G73620 56.250 224 91 4 23 241 40 261 2.85e-84 255
MS.gene057701.t1 AT4G38670 55.967 243 96 6 9 240 5 247 8.22e-84 254
MS.gene057701.t1 AT4G38670 55.967 243 96 6 9 240 5 247 8.22e-84 254
MS.gene057701.t1 AT4G38670 58.929 224 83 5 26 240 8 231 2.89e-83 252
MS.gene057701.t1 AT5G24620 52.419 248 102 6 25 257 25 271 6.27e-82 254
MS.gene057701.t1 AT5G24620 52.419 248 102 6 25 257 80 326 9.05e-82 255
MS.gene057701.t1 AT5G24620 52.419 248 102 6 25 257 101 347 1.10e-81 256
MS.gene057701.t1 AT4G36000 60.000 215 73 6 101 314 1 203 8.88e-77 234
MS.gene057701.t1 AT4G36000 60.000 215 73 6 101 314 1 203 8.88e-77 234
MS.gene057701.t1 AT1G77700 50.000 238 107 7 9 241 43 273 2.75e-74 231
MS.gene057701.t1 AT1G77700 49.793 241 109 7 6 241 40 273 8.48e-74 230
MS.gene057701.t1 AT1G77700 50.901 222 99 6 23 241 87 301 3.13e-73 230
MS.gene057701.t1 AT5G38280 47.131 244 113 5 21 248 21 264 1.10e-72 237
MS.gene057701.t1 AT5G38280 47.131 244 113 5 21 248 21 264 1.54e-72 236
MS.gene057701.t1 AT5G40020 44.082 245 124 4 10 242 7 250 5.10e-68 213
MS.gene057701.t1 AT2G28790 49.372 239 108 8 12 239 37 273 7.56e-66 208
MS.gene057701.t1 AT2G28790 49.372 239 108 8 12 239 12 248 9.61e-66 207
MS.gene057701.t1 AT5G02140 44.068 236 127 3 10 240 6 241 7.47e-64 204
MS.gene057701.t1 AT5G02140 44.068 236 127 3 10 240 6 241 9.03e-64 202
MS.gene057701.t1 AT4G11650 44.231 260 121 11 2 257 3 242 1.67e-53 176
MS.gene057701.t1 AT4G18253 39.004 241 138 3 9 245 8 243 1.95e-53 175
MS.gene057701.t1 AT1G70250 39.764 254 130 8 26 266 146 389 4.91e-52 183
MS.gene057701.t1 AT4G18250 40.506 237 132 5 23 254 55 287 1.10e-44 162
MS.gene057701.t1 AT5G24620 51.429 140 55 4 131 257 15 154 3.13e-39 140
MS.gene057701.t1 AT2G24810 61.039 77 27 1 166 239 116 192 8.54e-26 102

Find 103 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGGTTGGTCCGGCCGGTTC+TGG 0.121811 4.4:-35754465 MS.gene057701:CDS
TCTGCATTTGTTTACTCTTT+TGG 0.167211 4.4:-35753000 MS.gene057701:CDS
GTGAACTCAGCTAGGGTTGC+TGG 0.336395 4.4:+35754340 None:intergenic
CCAGGAGCAGGGACAGTGAT+AGG 0.340612 4.4:-35753216 MS.gene057701:CDS
ACTGTCCCTGCTCCTGGTAT+TGG 0.357647 4.4:+35753222 None:intergenic
TTTATTTACCTTGGAGAAGA+TGG 0.363744 4.4:+35753953 None:intergenic
CGGTTGGTCCGGCCGGTTCT+GGG 0.371851 4.4:-35754464 MS.gene057701:CDS
CCAGAACCGGCCGGACCAAC+CGG 0.372349 4.4:+35754465 None:intergenic
GGTATTGGTGTTGAGGTCAT+TGG 0.375177 4.4:+35753237 None:intergenic
GTTGAGGTCATTGGAGTTGT+TGG 0.375805 4.4:+35753246 None:intergenic
TTAACTCCGCTGGACATTGC+TGG 0.382982 4.4:+35754191 None:intergenic
CTCCAGCCGGTTGGTCCGGC+CGG 0.396644 4.4:-35754471 MS.gene057701:CDS
TACGACGTGAGTCTCGTTGA+TGG 0.402827 4.4:-35754292 MS.gene057701:CDS
GTTGGAACAGGATCTGAAAA+TGG 0.408945 4.4:-35753183 MS.gene057701:CDS
ACAAATGTGCCTACACAGTA+TGG 0.411373 4.4:-35754579 MS.gene057701:CDS
CAAGCTCCAGCCGGTTGGTC+CGG 0.411921 4.4:-35754475 MS.gene057701:CDS
GGCCGGTTCTGGGCTCGAAC+CGG 0.412457 4.4:-35754454 MS.gene057701:CDS
TAGGCGTAGCTATACGATCT+AGG 0.413391 4.4:+35754034 None:intergenic
AAGAGCGCGTGTGAGGCTTT+TGG 0.414184 4.4:-35754145 MS.gene057701:CDS
AAACCGGTGCTTCCGAGTGT+TGG 0.422655 4.4:+35754529 None:intergenic
TCAGATCCTGTTCCAACCTC+AGG 0.424740 4.4:+35753189 None:intergenic
AACCGGTGCTTCCGAGTGTT+GGG 0.429937 4.4:+35754530 None:intergenic
TCGGGTATGTGTGAGTCCAC+CGG 0.437031 4.4:-35754232 MS.gene057701:CDS
ACCAGAGCCTTCAACAATCA+TGG 0.440414 4.4:+35754258 None:intergenic
AACCGGTTCGAGCCCAGAAC+CGG 0.440678 4.4:+35754452 None:intergenic
CGCGCCACTACAACAATACT+CGG 0.445194 4.4:+35754117 None:intergenic
TTCTTGTGGAAGCTCGAAAC+CGG 0.455126 4.4:+35754513 None:intergenic
CCTATCACTGTCCCTGCTCC+TGG 0.456736 4.4:+35753216 None:intergenic
GAGTTCACTCTTGGCACTAT+AGG 0.458610 4.4:-35754325 MS.gene057701:CDS
TGGATCATATTTAGCTGGAT+TGG 0.461151 4.4:-35753052 MS.gene057701:CDS
GTTGAAGGCTCTGGTGGGTC+GGG 0.465729 4.4:-35754250 MS.gene057701:CDS
AATCTACCCATGATTGTTGA+AGG 0.467465 4.4:-35754265 MS.gene057701:CDS
AACTTCGACGGATCCGGCTC+CGG 0.467634 4.4:-35754427 MS.gene057701:CDS
AACCGGCCGGACCAACCGGC+TGG 0.468762 4.4:+35754469 None:intergenic
TTGTAGACCGTCGATTTACT+CGG 0.469194 4.4:-35754077 MS.gene057701:CDS
CGGGTATGTGTGAGTCCACC+GGG 0.470901 4.4:-35754231 MS.gene057701:CDS
CCCATGATTGTTGAAGGCTC+TGG 0.472347 4.4:-35754259 MS.gene057701:CDS
TATTGTTGTAGTGGCGCGTA+TGG 0.473651 4.4:-35754112 MS.gene057701:CDS
GGTTGCTGGTGGTGCAGCTC+CGG 0.475246 4.4:+35754354 None:intergenic
GTCTCCGGTAAGACATGAAC+CGG 0.475517 4.4:+35754408 None:intergenic
GGTTGCAACTTCGACGGATC+CGG 0.475598 4.4:-35754433 MS.gene057701:CDS
GGGACTCCTGAGGTTGGAAC+AGG 0.482015 4.4:-35753195 MS.gene057701:CDS
CCTTGGAGAAGATGGACAAA+AGG 0.482274 4.4:+35753961 None:intergenic
GCAGATGGATCATATTTAGC+TGG 0.484088 4.4:-35753057 MS.gene057701:intron
CGCACTTTCCAAGCTCCAGC+CGG 0.485696 4.4:-35754484 MS.gene057701:CDS
CCTGCATTTGCTAGAATACC+TGG 0.487206 4.4:+35754556 None:intergenic
CGGACCAACCGGCTGGAGCT+TGG 0.491158 4.4:+35754476 None:intergenic
TGTTGAAGGCTCTGGTGGGT+CGG 0.495865 4.4:-35754251 MS.gene057701:CDS
GGCTCCGGTTCATGTCTTAC+CGG 0.498319 4.4:-35754412 MS.gene057701:CDS
CATGTTTGAGCCACAGTCTC+CGG 0.506120 4.4:+35754393 None:intergenic
TGCTCCTGGTATTGGTGTTG+AGG 0.514274 4.4:+35753230 None:intergenic
TCTGAAGCTCTTAACTCCGC+TGG 0.518210 4.4:+35754181 None:intergenic
ATGATTGTTGAAGGCTCTGG+TGG 0.522224 4.4:-35754256 MS.gene057701:CDS
GGTAAGACATGAACCGGAGC+CGG 0.526054 4.4:+35754414 None:intergenic
ACCCTAGCTGAGTTCACTCT+TGG 0.527571 4.4:-35754334 MS.gene057701:CDS
ATGGTAGAATGCAACGGTGC+CGG 0.536138 4.4:-35754373 MS.gene057701:CDS
GGATCCGTCGAAGTTGCAAC+CGG 0.540266 4.4:+35754435 None:intergenic
GTGATAGGGACTCCTGAGGT+TGG 0.541020 4.4:-35753201 MS.gene057701:CDS
AGCCCAACACTCGGAAGCAC+CGG 0.545623 4.4:-35754532 MS.gene057701:CDS
CTTTCCAAGCTCCAGCCGGT+TGG 0.546315 4.4:-35754480 MS.gene057701:CDS
AAAGAGAGTGATATAAGTTG+AGG 0.549260 4.4:+35754753 None:intergenic
CGCGTGTAAGAGCGCGTGTG+AGG 0.556474 4.4:-35754152 MS.gene057701:CDS
TGCCAAGAGTGAACTCAGCT+AGG 0.556861 4.4:+35754332 None:intergenic
TCAACACCAATACCAGGAGC+AGG 0.558407 4.4:-35753228 MS.gene057701:CDS
AGGCGTAGCTATACGATCTA+GGG 0.561666 4.4:+35754035 None:intergenic
GCCAAGAGTGAACTCAGCTA+GGG 0.562984 4.4:+35754333 None:intergenic
TATTTAGCTGGATTGGCCAT+GGG 0.568640 4.4:-35753045 MS.gene057701:CDS
CAGGAGCAGGGACAGTGATA+GGG 0.571920 4.4:-35753215 MS.gene057701:CDS
AACATTTCCGAGTAAATCGA+CGG 0.574542 4.4:+35754070 None:intergenic
CGGAGACTGTGGCTCAAACA+TGG 0.576529 4.4:-35754392 MS.gene057701:CDS
CCAGAGCCTTCAACAATCAT+GGG 0.576787 4.4:+35754259 None:intergenic
TGTGCCTACACAGTATGGCC+AGG 0.579329 4.4:-35754574 MS.gene057701:CDS
CCAGGTATTCTAGCAAATGC+AGG 0.579356 4.4:-35754556 MS.gene057701:CDS
TGCAGATGAAGTTGTTGTAG+AGG 0.581360 4.4:+35753017 None:intergenic
TAGTGGCGCGTATGGTTCAC+CGG 0.583066 4.4:-35754104 MS.gene057701:CDS
ACATTTGTTTACAAATGTGA+AGG 0.586021 4.4:+35754594 None:intergenic
ATATTTAGCTGGATTGGCCA+TGG 0.590981 4.4:-35753046 MS.gene057701:CDS
GTTCAGATCGGAAGTACACC+CGG 0.591972 4.4:+35754213 None:intergenic
GCGGAGTTAAGAGCTTCAGA+TGG 0.606315 4.4:-35754178 MS.gene057701:CDS
TGGACATTGCTGGTTCAGAT+CGG 0.606939 4.4:+35754201 None:intergenic
GGTTCGAGCCCAGAACCGGC+CGG 0.616663 4.4:+35754456 None:intergenic
ATGACCTCAACACCAATACC+AGG 0.616871 4.4:-35753234 MS.gene057701:CDS
ATCGACGGTCTACAAGTAGC+CGG 0.622951 4.4:+35754085 None:intergenic
AATGCAGGTAGCCCAACACT+CGG 0.635714 4.4:-35754541 MS.gene057701:CDS
AGTCCCGAGTATTGTTGTAG+TGG 0.636472 4.4:-35754121 MS.gene057701:CDS
AATACCTGGCCATACTGTGT+AGG 0.637300 4.4:+35754570 None:intergenic
CGAACCGGTTGCAACTTCGA+CGG 0.637688 4.4:-35754439 MS.gene057701:CDS
TGGAGCTTGGAAAGTGCGAG+TGG 0.641087 4.4:+35754489 None:intergenic
TGTTGTAGAGGAGTCTCCCA+TGG 0.642923 4.4:+35753029 None:intergenic
GCGCCACTACAACAATACTC+GGG 0.652627 4.4:+35754118 None:intergenic
TCAAACATGGTAGAATGCAA+CGG 0.654931 4.4:-35754379 MS.gene057701:CDS
AGAAGATGGACAAAAGGTAA+CGG 0.655103 4.4:+35753967 None:intergenic
AACTCAGCTAGGGTTGCTGG+TGG 0.659719 4.4:+35754343 None:intergenic
CAACACCAATACCAGGAGCA+GGG 0.663691 4.4:-35753227 MS.gene057701:CDS
AGTACTCGAAGCATCATCGT+AGG 0.663812 4.4:+35754015 None:intergenic
TCATGTCTTACCGGAGACTG+TGG 0.669388 4.4:-35754403 MS.gene057701:CDS
TGATTGTTGAAGGCTCTGGT+GGG 0.669561 4.4:-35754255 MS.gene057701:CDS
TGAAAATGGAGTTCAATACA+CGG 0.669710 4.4:-35753169 MS.gene057701:CDS
AAAGTGCGAGTGGTTTCTTG+TGG 0.674516 4.4:+35754499 None:intergenic
GAAAATGGAGTTCAATACAC+GGG 0.685341 4.4:-35753168 MS.gene057701:intron
TCTGAACCAGCAATGTCCAG+CGG 0.703644 4.4:-35754197 MS.gene057701:CDS
GACAGTGATAGGGACTCCTG+AGG 0.705413 4.4:-35753205 MS.gene057701:CDS
CAGATCGGAAGTACACCCGG+TGG 0.809112 4.4:+35754216 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGATTAATTTTATTTACCT+TGG + chr4.4:35753797-35753816 None:intergenic 15.0%
!! AATAATGAAAACATAGACAA+AGG + chr4.4:35753048-35753067 None:intergenic 20.0%
!! ATTAATAGTAGATTTATCGT+TGG - chr4.4:35754195-35754214 MS.gene057701:CDS 20.0%
!!! AATTTTGTGATTGTAGAAAT+TGG - chr4.4:35754417-35754436 MS.gene057701:CDS 20.0%
!!! ATGTTAAAAGTATGTAAACT+AGG - chr4.4:35753834-35753853 MS.gene057701:intron 20.0%
!!! TGTTAAAAGTATGTAAACTA+GGG - chr4.4:35753835-35753854 MS.gene057701:intron 20.0%
!!! TTTTTTACATGTTTTTGTTC+AGG - chr4.4:35753107-35753126 MS.gene057701:intron 20.0%
! ACATTTGTTTACAAATGTGA+AGG + chr4.4:35753147-35753166 None:intergenic 25.0%
! GTCAGAAATTATTCTTCAAA+AGG + chr4.4:35753956-35753975 None:intergenic 25.0%
! TAACAACATTGTCGTATAAT+GGG + chr4.4:35753862-35753881 None:intergenic 25.0%
! TTAACAACATTGTCGTATAA+TGG + chr4.4:35753863-35753882 None:intergenic 25.0%
!! CAAGAAAAAGTTAGTGTTTT+TGG + chr4.4:35753084-35753103 None:intergenic 25.0%
AAAGAGAGTGATATAAGTTG+AGG + chr4.4:35752988-35753007 None:intergenic 30.0%
AAAGTCATGTAATGTCATCA+TGG - chr4.4:35754056-35754075 MS.gene057701:CDS 30.0%
AACTAGTCATCTTAAGCAAA+CGG - chr4.4:35754254-35754273 MS.gene057701:CDS 30.0%
ATCTTAAGCAAACGGTTAAA+TGG - chr4.4:35754262-35754281 MS.gene057701:CDS 30.0%
ATTTCCACTTTCAGATTTGT+AGG - chr4.4:35754110-35754129 MS.gene057701:CDS 30.0%
GATTGTAACAACTTGTGATA+TGG - chr4.4:35754442-35754461 MS.gene057701:CDS 30.0%
TGAAAATGGAGTTCAATACA+CGG - chr4.4:35754569-35754588 MS.gene057701:CDS 30.0%
TTAAAAACTATGTTCCCCTT+TGG + chr4.4:35754165-35754184 None:intergenic 30.0%
TTTATTTACCTTGGAGAAGA+TGG + chr4.4:35753788-35753807 None:intergenic 30.0%
TTTCCACTTTCAGATTTGTA+GGG - chr4.4:35754111-35754130 MS.gene057701:CDS 30.0%
! AAGGGGAACATAGTTTTTAA+TGG - chr4.4:35754165-35754184 MS.gene057701:CDS 30.0%
! TCTGCATTTGTTTACTCTTT+TGG - chr4.4:35754738-35754757 MS.gene057701:CDS 30.0%
!! AAAGGTTGTGTTTTTACCTT+TGG + chr4.4:35753030-35753049 None:intergenic 30.0%
!! AAGGTTGTGTTTTTACCTTT+GGG + chr4.4:35753029-35753048 None:intergenic 30.0%
!! TCGTTGGCATTTAATTTGTT+AGG - chr4.4:35754211-35754230 MS.gene057701:CDS 30.0%
!!! GTTAGTGTTTTTGGTTTTGA+AGG + chr4.4:35753075-35753094 None:intergenic 30.0%
!!! TGTTTTTTCCATGTAACCAT+AGG - chr4.4:35754136-35754155 MS.gene057701:CDS 30.0%
AAACCCTACAAATCTGAAAG+TGG + chr4.4:35754117-35754136 None:intergenic 35.0%
AACATTTCCGAGTAAATCGA+CGG + chr4.4:35753671-35753690 None:intergenic 35.0%
AATCTACCCATGATTGTTGA+AGG - chr4.4:35753473-35753492 MS.gene057701:intron 35.0%
AGAAGATGGACAAAAGGTAA+CGG + chr4.4:35753774-35753793 None:intergenic 35.0%
ATAAAATCCCACTTGCTCAA+AGG - chr4.4:35754355-35754374 MS.gene057701:CDS 35.0%
ATTCAGTTCCCGTAAATTCA+GGG - chr4.4:35754613-35754632 MS.gene057701:intron 35.0%
GAAAATGGAGTTCAATACAC+GGG - chr4.4:35754570-35754589 MS.gene057701:CDS 35.0%
TAAAATCCCACTTGCTCAAA+GGG - chr4.4:35754356-35754375 MS.gene057701:CDS 35.0%
TATTCAGTTCCCGTAAATTC+AGG - chr4.4:35754612-35754631 MS.gene057701:CDS 35.0%
TCAAACATGGTAGAATGCAA+CGG - chr4.4:35753359-35753378 MS.gene057701:intron 35.0%
TGGATCATATTTAGCTGGAT+TGG - chr4.4:35754686-35754705 MS.gene057701:intron 35.0%
! TACTTTTACTTGTACTGCTG+CGG - chr4.4:35753742-35753761 MS.gene057701:intron 35.0%
!! AGGTTGTGTTTTTACCTTTG+GGG + chr4.4:35753028-35753047 None:intergenic 35.0%
!! TGTTTTTGTTCAGGTATCAC+TGG - chr4.4:35753116-35753135 MS.gene057701:intron 35.0%
ACAAATGTGCCTACACAGTA+TGG - chr4.4:35753159-35753178 MS.gene057701:intron 40.0%
ACAATACCCTTTGAGCAAGT+GGG + chr4.4:35754365-35754384 None:intergenic 40.0%
ATATGATCCATCTGCAAGCA+TGG + chr4.4:35754676-35754695 None:intergenic 40.0%
ATGTAACCATAGGTAGCCAA+AGG - chr4.4:35754146-35754165 MS.gene057701:CDS 40.0%
GCAGATGGATCATATTTAGC+TGG - chr4.4:35754681-35754700 MS.gene057701:intron 40.0%
GTTGGAACAGGATCTGAAAA+TGG - chr4.4:35754555-35754574 MS.gene057701:CDS 40.0%
TACAATACCCTTTGAGCAAG+TGG + chr4.4:35754366-35754385 None:intergenic 40.0%
TCAGCATCTAATTCAGGATC+AGG - chr4.4:35754642-35754661 MS.gene057701:intron 40.0%
TCCTGAATTAGATGCTGATC+CGG + chr4.4:35754640-35754659 None:intergenic 40.0%
TGCAGATGAAGTTGTTGTAG+AGG + chr4.4:35754724-35754743 None:intergenic 40.0%
TGTAACCATAGGTAGCCAAA+GGG - chr4.4:35754147-35754166 MS.gene057701:CDS 40.0%
TTTGGCTACCTATGGTTACA+TGG + chr4.4:35754147-35754166 None:intergenic 40.0%
!! ATATTTAGCTGGATTGGCCA+TGG - chr4.4:35754692-35754711 MS.gene057701:intron 40.0%
!! GGTTGTGTTTTTACCTTTGG+GGG + chr4.4:35753027-35753046 None:intergenic 40.0%
!! TATTTAGCTGGATTGGCCAT+GGG - chr4.4:35754693-35754712 MS.gene057701:intron 40.0%
!! TTGTAGACCGTCGATTTACT+CGG - chr4.4:35753661-35753680 MS.gene057701:intron 40.0%
AAAGTGCGAGTGGTTTCTTG+TGG + chr4.4:35753242-35753261 None:intergenic 45.0%
AATACCTGGCCATACTGTGT+AGG + chr4.4:35753171-35753190 None:intergenic 45.0%
ACCAGAGCCTTCAACAATCA+TGG + chr4.4:35753483-35753502 None:intergenic 45.0%
ACGGGAACTGAATACCCAAT+GGG + chr4.4:35754606-35754625 None:intergenic 45.0%
AGGCGTAGCTATACGATCTA+GGG + chr4.4:35753706-35753725 None:intergenic 45.0%
ATGACCTCAACACCAATACC+AGG - chr4.4:35754504-35754523 MS.gene057701:CDS 45.0%
CCAGAGCCTTCAACAATCAT+GGG + chr4.4:35753482-35753501 None:intergenic 45.0%
CCAGGTATTCTAGCAAATGC+AGG - chr4.4:35753182-35753201 MS.gene057701:CDS 45.0%
CCTTGGAGAAGATGGACAAA+AGG + chr4.4:35753780-35753799 None:intergenic 45.0%
GAGTTCACTCTTGGCACTAT+AGG - chr4.4:35753413-35753432 MS.gene057701:intron 45.0%
GCATCTAATTCAGGATCAGG+AGG - chr4.4:35754645-35754664 MS.gene057701:intron 45.0%
GTAACCATAGGTAGCCAAAG+GGG - chr4.4:35754148-35754167 MS.gene057701:CDS 45.0%
GTTGAGGTCATTGGAGTTGT+TGG + chr4.4:35754495-35754514 None:intergenic 45.0%
TACGGGAACTGAATACCCAA+TGG + chr4.4:35754607-35754626 None:intergenic 45.0%
TAGGCGTAGCTATACGATCT+AGG + chr4.4:35753707-35753726 None:intergenic 45.0%
TATTGTTGTAGTGGCGCGTA+TGG - chr4.4:35753626-35753645 MS.gene057701:intron 45.0%
TCCGGATCAGCATCTAATTC+AGG - chr4.4:35754636-35754655 MS.gene057701:intron 45.0%
TGATCCGGACCCTGAATTTA+CGG + chr4.4:35754625-35754644 None:intergenic 45.0%
TTCTTGTGGAAGCTCGAAAC+CGG + chr4.4:35753228-35753247 None:intergenic 45.0%
! AGTACTCGAAGCATCATCGT+AGG + chr4.4:35753726-35753745 None:intergenic 45.0%
! AGTCCCGAGTATTGTTGTAG+TGG - chr4.4:35753617-35753636 MS.gene057701:intron 45.0%
! ATGATTGTTGAAGGCTCTGG+TGG - chr4.4:35753482-35753501 MS.gene057701:intron 45.0%
! CCTGCATTTGCTAGAATACC+TGG + chr4.4:35753185-35753204 None:intergenic 45.0%
! CCTTTTGTCCATCTTCTCCA+AGG - chr4.4:35753777-35753796 MS.gene057701:intron 45.0%
! TGATTGTTGAAGGCTCTGGT+GGG - chr4.4:35753483-35753502 MS.gene057701:intron 45.0%
!! GGTATTGGTGTTGAGGTCAT+TGG + chr4.4:35754504-35754523 None:intergenic 45.0%
!! TGGACATTGCTGGTTCAGAT+CGG + chr4.4:35753540-35753559 None:intergenic 45.0%
!! TGTTTTTACCTTTGGGGGTG+AGG + chr4.4:35753022-35753041 None:intergenic 45.0%
AATGCAGGTAGCCCAACACT+CGG - chr4.4:35753197-35753216 MS.gene057701:CDS 50.0%
ACCCTAGCTGAGTTCACTCT+TGG - chr4.4:35753404-35753423 MS.gene057701:intron 50.0%
ATCGACGGTCTACAAGTAGC+CGG + chr4.4:35753656-35753675 None:intergenic 50.0%
ATGGTAGAATGCAACGGTGC+CGG - chr4.4:35753365-35753384 MS.gene057701:intron 50.0%
CAACACCAATACCAGGAGCA+GGG - chr4.4:35754511-35754530 MS.gene057701:CDS 50.0%
CATGTTTGAGCCACAGTCTC+CGG + chr4.4:35753348-35753367 None:intergenic 50.0%
CCCATGATTGTTGAAGGCTC+TGG - chr4.4:35753479-35753498 MS.gene057701:intron 50.0%
CGCGCCACTACAACAATACT+CGG + chr4.4:35753624-35753643 None:intergenic 50.0%
CTTCTTATCCTCACCCCCAA+AGG - chr4.4:35753011-35753030 MS.gene057701:CDS 50.0%
GATCCGGACCCTGAATTTAC+GGG + chr4.4:35754624-35754643 None:intergenic 50.0%
GCCAAGAGTGAACTCAGCTA+GGG + chr4.4:35753408-35753427 None:intergenic 50.0%
GCGCCACTACAACAATACTC+GGG + chr4.4:35753623-35753642 None:intergenic 50.0%
GCGGAGTTAAGAGCTTCAGA+TGG - chr4.4:35753560-35753579 MS.gene057701:intron 50.0%
GGAGAAACCATGCTTGCAGA+TGG - chr4.4:35754666-35754685 MS.gene057701:intron 50.0%
GTCTCCGGTAAGACATGAAC+CGG + chr4.4:35753333-35753352 None:intergenic 50.0%
GTTCCCGTAAATTCAGGGTC+CGG - chr4.4:35754618-35754637 MS.gene057701:intron 50.0%
TACGACGTGAGTCTCGTTGA+TGG - chr4.4:35753446-35753465 MS.gene057701:intron 50.0%
TCAACACCAATACCAGGAGC+AGG - chr4.4:35754510-35754529 MS.gene057701:CDS 50.0%
TCAGATCCTGTTCCAACCTC+AGG + chr4.4:35754552-35754571 None:intergenic 50.0%
TCATGTCTTACCGGAGACTG+TGG - chr4.4:35753335-35753354 MS.gene057701:intron 50.0%
TCTGAACCAGCAATGTCCAG+CGG - chr4.4:35753541-35753560 MS.gene057701:intron 50.0%
TGCCAAGAGTGAACTCAGCT+AGG + chr4.4:35753409-35753428 None:intergenic 50.0%
TGTTCCCCTTTGGCTACCTA+TGG + chr4.4:35754155-35754174 None:intergenic 50.0%
TGTTGTAGAGGAGTCTCCCA+TGG + chr4.4:35754712-35754731 None:intergenic 50.0%
TTAACTCCGCTGGACATTGC+TGG + chr4.4:35753550-35753569 None:intergenic 50.0%
! GTTCAGATCGGAAGTACACC+CGG + chr4.4:35753528-35753547 None:intergenic 50.0%
! TCTGAAGCTCTTAACTCCGC+TGG + chr4.4:35753560-35753579 None:intergenic 50.0%
!! TGCTCCTGGTATTGGTGTTG+AGG + chr4.4:35754511-35754530 None:intergenic 50.0%
AAACCGGTGCTTCCGAGTGT+TGG + chr4.4:35753212-35753231 None:intergenic 55.0%
AACCGGTGCTTCCGAGTGTT+GGG + chr4.4:35753211-35753230 None:intergenic 55.0%
AACTCAGCTAGGGTTGCTGG+TGG + chr4.4:35753398-35753417 None:intergenic 55.0%
ACGGGTACAGACACACCCAT+TGG - chr4.4:35754588-35754607 MS.gene057701:CDS 55.0%
ACTGTCCCTGCTCCTGGTAT+TGG + chr4.4:35754519-35754538 None:intergenic 55.0%
CAGGAGCAGGGACAGTGATA+GGG - chr4.4:35754523-35754542 MS.gene057701:CDS 55.0%
CGAACCGGTTGCAACTTCGA+CGG - chr4.4:35753299-35753318 MS.gene057701:intron 55.0%
CGGAGACTGTGGCTCAAACA+TGG - chr4.4:35753346-35753365 MS.gene057701:intron 55.0%
CGGGTACAGACACACCCATT+GGG - chr4.4:35754589-35754608 MS.gene057701:CDS 55.0%
GACAGTGATAGGGACTCCTG+AGG - chr4.4:35754533-35754552 MS.gene057701:CDS 55.0%
GGATCCGTCGAAGTTGCAAC+CGG + chr4.4:35753306-35753325 None:intergenic 55.0%
GGCTCCGGTTCATGTCTTAC+CGG - chr4.4:35753326-35753345 MS.gene057701:intron 55.0%
GGTAAGACATGAACCGGAGC+CGG + chr4.4:35753327-35753346 None:intergenic 55.0%
GGTTGCAACTTCGACGGATC+CGG - chr4.4:35753305-35753324 MS.gene057701:intron 55.0%
GTGAACTCAGCTAGGGTTGC+TGG + chr4.4:35753401-35753420 None:intergenic 55.0%
GTGATAGGGACTCCTGAGGT+TGG - chr4.4:35754537-35754556 MS.gene057701:CDS 55.0%
TAGTGGCGCGTATGGTTCAC+CGG - chr4.4:35753634-35753653 MS.gene057701:intron 55.0%
TCGGGTATGTGTGAGTCCAC+CGG - chr4.4:35753506-35753525 MS.gene057701:intron 55.0%
TGTGCCTACACAGTATGGCC+AGG - chr4.4:35753164-35753183 MS.gene057701:intron 55.0%
! TGGAGCTTGGAAAGTGCGAG+TGG + chr4.4:35753252-35753271 None:intergenic 55.0%
! TGTTGAAGGCTCTGGTGGGT+CGG - chr4.4:35753487-35753506 MS.gene057701:intron 55.0%
!! AAGAGCGCGTGTGAGGCTTT+TGG - chr4.4:35753593-35753612 MS.gene057701:intron 55.0%
AACTTCGACGGATCCGGCTC+CGG - chr4.4:35753311-35753330 MS.gene057701:intron 60.0%
CCAGGAGCAGGGACAGTGAT+AGG - chr4.4:35754522-35754541 MS.gene057701:CDS 60.0%
CCTATCACTGTCCCTGCTCC+TGG + chr4.4:35754525-35754544 None:intergenic 60.0%
CGCACTTTCCAAGCTCCAGC+CGG - chr4.4:35753254-35753273 MS.gene057701:CDS 60.0%
CGGGTATGTGTGAGTCCACC+GGG - chr4.4:35753507-35753526 MS.gene057701:intron 60.0%
CTTTCCAAGCTCCAGCCGGT+TGG - chr4.4:35753258-35753277 MS.gene057701:CDS 60.0%
GGGACTCCTGAGGTTGGAAC+AGG - chr4.4:35754543-35754562 MS.gene057701:CDS 60.0%
! AGCCCAACACTCGGAAGCAC+CGG - chr4.4:35753206-35753225 MS.gene057701:CDS 60.0%
! CAGATCGGAAGTACACCCGG+TGG + chr4.4:35753525-35753544 None:intergenic 60.0%
! GTTGAAGGCTCTGGTGGGTC+GGG - chr4.4:35753488-35753507 MS.gene057701:intron 60.0%
!! AACCGGTTCGAGCCCAGAAC+CGG + chr4.4:35753289-35753308 None:intergenic 60.0%
! CAAGCTCCAGCCGGTTGGTC+CGG - chr4.4:35753263-35753282 MS.gene057701:CDS 65.0%
! CGCGTGTAAGAGCGCGTGTG+AGG - chr4.4:35753586-35753605 MS.gene057701:intron 65.0%
! GGTTGCTGGTGGTGCAGCTC+CGG + chr4.4:35753387-35753406 None:intergenic 65.0%
CCAGAACCGGCCGGACCAAC+CGG + chr4.4:35753276-35753295 None:intergenic 70.0%
CGGACCAACCGGCTGGAGCT+TGG + chr4.4:35753265-35753284 None:intergenic 70.0%
GGTTCGAGCCCAGAACCGGC+CGG + chr4.4:35753285-35753304 None:intergenic 70.0%
! CGGTTGGTCCGGCCGGTTCT+GGG - chr4.4:35753274-35753293 MS.gene057701:intron 70.0%
!! GGCCGGTTCTGGGCTCGAAC+CGG - chr4.4:35753284-35753303 MS.gene057701:intron 70.0%
AACCGGCCGGACCAACCGGC+TGG + chr4.4:35753272-35753291 None:intergenic 75.0%
! CCGGTTGGTCCGGCCGGTTC+TGG - chr4.4:35753273-35753292 MS.gene057701:intron 75.0%
! CTCCAGCCGGTTGGTCCGGC+CGG - chr4.4:35753267-35753286 MS.gene057701:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 35752972 35754788 35752972 ID=MS.gene057701
chr4.4 mRNA 35752972 35754788 35752972 ID=MS.gene057701.t1;Parent=MS.gene057701
chr4.4 exon 35754728 35754788 35754728 ID=MS.gene057701.t1.exon1;Parent=MS.gene057701.t1
chr4.4 CDS 35754728 35754788 35754728 ID=cds.MS.gene057701.t1;Parent=MS.gene057701.t1
chr4.4 exon 35753962 35754631 35753962 ID=MS.gene057701.t1.exon2;Parent=MS.gene057701.t1
chr4.4 CDS 35753962 35754631 35753962 ID=cds.MS.gene057701.t1;Parent=MS.gene057701.t1
chr4.4 exon 35753169 35753287 35753169 ID=MS.gene057701.t1.exon3;Parent=MS.gene057701.t1
chr4.4 CDS 35753169 35753287 35753169 ID=cds.MS.gene057701.t1;Parent=MS.gene057701.t1
chr4.4 exon 35752972 35753075 35752972 ID=MS.gene057701.t1.exon4;Parent=MS.gene057701.t1
chr4.4 CDS 35752972 35753075 35752972 ID=cds.MS.gene057701.t1;Parent=MS.gene057701.t1
Gene Sequence

>MS.gene057701

ATGGATCTACTAGCCTCAACTTATATCACTCTCTTTACTCTTCTTATCCTCACCCCCAAAGGTAAAAACACAACCTTTGTCTATGTTTTCATTATTTTCACCTTCAAAACCAAAAACACTAACTTTTTCTTGTTTTTTTTTACATGTTTTTGTTCAGGTATCACTGGTTCAACCTTCACATTTGTAAACAAATGTGCCTACACAGTATGGCCAGGTATTCTAGCAAATGCAGGTAGCCCAACACTCGGAAGCACCGGTTTCGAGCTTCCACAAGAAACCACTCGCACTTTCCAAGCTCCAGCCGGTTGGTCCGGCCGGTTCTGGGCTCGAACCGGTTGCAACTTCGACGGATCCGGCTCCGGTTCATGTCTTACCGGAGACTGTGGCTCAAACATGGTAGAATGCAACGGTGCCGGAGCTGCACCACCAGCAACCCTAGCTGAGTTCACTCTTGGCACTATAGGACAAGATTTCTACGACGTGAGTCTCGTTGATGGCTACAATCTACCCATGATTGTTGAAGGCTCTGGTGGGTCGGGTATGTGTGAGTCCACCGGGTGTACTTCCGATCTGAACCAGCAATGTCCAGCGGAGTTAAGAGCTTCAGATGGAAGCGCGTGTAAGAGCGCGTGTGAGGCTTTTGGTAGTCCCGAGTATTGTTGTAGTGGCGCGTATGGTTCACCGGCTACTTGTAGACCGTCGATTTACTCGGAAATGTTTAAAGCTGCTTGCCCTAGATCGTATAGCTACGCCTACGATGATGCTTCGAGTACTTTTACTTGTACTGCTGCGGATTATACCGTTACCTTTTGTCCATCTTCTCCAAGGTAAATAAAATTAATCAACACTAATCTCTTTTTTTATGTTAAAAGTATGTAAACTAGGGTCCCATTATACGACAATGTTGTTAAAAAATCAACAAGTGATATAATTGTATATTGAAAAGTAAAACATAATAGTGATAAAGTGTTAAATATTTTACCTTTTGAAGAATAATTTCTGACATTTGTGAATATTTGTTCGTATAATTTGTAATTTATGATTTAATAACTTTTAGAGATATGTTTGTTTATAGTTTTGTTGAAAAGTCATGTAATGTCATCATGGACACGCTTTACTTTAGTATTTGAGTGTAAGAATTTCCACTTTCAGATTTGTAGGGTTTGTTTTTTCCATGTAACCATAGGTAGCCAAAGGGGAACATAGTTTTTAATGGCTGTGATATTAATAGTAGATTTATCGTTGGCATTTAATTTGTTAGGTGAATCATAATGACGAGTCTAACTAGTCATCTTAAGCAAACGGTTAAATGGTGTGCATGTAACTAGATTATCTAAAAAAGTCTAATGAGTGTAATGAGTAACGTTAGATTTGACATTGTTGATAAAATCCCACTTGCTCAAAGGGTATTGTAAGTAATATCATTAGTACTTAGTAGTACTTACAATTTTGTGATTGTAGAAATTGGTTGATTGTAACAACTTGTGATATGGTTTTGCAGTAAAAAATCATCACAATATCCAACAACTCCAATGACCTCAACACCAATACCAGGAGCAGGGACAGTGATAGGGACTCCTGAGGTTGGAACAGGATCTGAAAATGGAGTTCAATACACGGGTACAGACACACCCATTGGGTATTCAGTTCCCGTAAATTCAGGGTCCGGATCAGCATCTAATTCAGGATCAGGAGGAGAAACCATGCTTGCAGATGGATCATATTTAGCTGGATTGGCCATGGGAGACTCCTCTACAACAACTTCATCTGCATTTGTTTACTCTTTTGGTTTTTTTCTTATTTTCTTCTTGCTTTAG
Protein sequence

>MS.gene057701.t1

MDLLASTYITLFTLLILTPKGITGSTFTFVNKCAYTVWPGILANAGSPTLGSTGFELPQETTRTFQAPAGWSGRFWARTGCNFDGSGSGSCLTGDCGSNMVECNGAGAAPPATLAEFTLGTIGQDFYDVSLVDGYNLPMIVEGSGGSGMCESTGCTSDLNQQCPAELRASDGSACKSACEAFGSPEYCCSGAYGSPATCRPSIYSEMFKAACPRSYSYAYDDASSTFTCTAADYTVTFCPSSPSKKSSQYPTTPMTSTPIPGAGTVIGTPEVGTGSENGVQYTDGSYLAGLAMGDSSTTTSSAFVYSFGFFLIFFLL