AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057882

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057882.t1 MTR_5g085580 97.938 291 5 1 1 290 1 291 0.0 571
MS.gene057882.t1 MTR_3g080410 74.725 273 69 0 18 290 20 292 2.22e-151 428
MS.gene057882.t1 MTR_1g056640 64.815 270 95 0 21 290 35 304 7.53e-126 368
MS.gene057882.t1 MTR_1g077990 48.352 273 138 3 19 290 26 296 8.07e-79 245
MS.gene057882.t1 MTR_4g084480 45.486 288 154 3 4 290 5 290 9.23e-79 244
MS.gene057882.t1 MTR_4g084480 45.486 288 154 3 4 290 12 297 1.01e-78 244
MS.gene057882.t1 MTR_4g084480 45.486 288 154 3 4 290 12 297 1.08e-78 244
MS.gene057882.t1 MTR_4g010200 42.268 291 157 3 8 288 8 297 1.93e-77 241
MS.gene057882.t1 MTR_3g065460 44.138 290 154 3 9 291 5 293 4.52e-75 238
MS.gene057882.t1 MTR_8g012400 41.216 296 171 3 6 299 11 305 2.28e-74 235
MS.gene057882.t1 MTR_4g069940 44.238 269 148 2 24 291 29 296 3.12e-74 235
MS.gene057882.t1 MTR_4g088155 40.741 297 167 4 11 299 6 301 3.17e-74 235
MS.gene057882.t1 MTR_1g031900 42.907 289 161 2 6 291 6 293 1.70e-73 234
MS.gene057882.t1 MTR_1g031900 42.907 289 161 2 6 291 6 293 1.80e-73 234
MS.gene057882.t1 MTR_4g052970 44.526 274 145 4 20 290 25 294 6.70e-73 229
MS.gene057882.t1 MTR_4g132280 43.173 271 150 3 24 291 45 314 1.84e-72 232
MS.gene057882.t1 MTR_2g086530 41.237 291 160 4 7 290 6 292 1.39e-71 226
MS.gene057882.t1 MTR_2g086530 43.494 269 147 3 23 290 7 271 1.15e-70 223
MS.gene057882.t1 MTR_3g116510 41.522 289 164 3 7 291 8 295 1.39e-70 225
MS.gene057882.t1 MTR_2g019345 44.561 285 153 5 8 290 7 288 2.71e-70 223
MS.gene057882.t1 MTR_7g053500 42.164 268 152 3 25 290 27 293 3.64e-70 224
MS.gene057882.t1 MTR_6g088885 39.858 281 168 1 11 291 14 293 3.02e-69 223
MS.gene057882.t1 MTR_5g078200 42.808 292 161 3 3 291 4 292 4.00e-69 226
MS.gene057882.t1 MTR_1g007810 42.756 283 154 5 11 291 4 280 4.53e-68 216
MS.gene057882.t1 MTR_1g022160 39.350 277 165 3 24 298 24 299 8.72e-68 217
MS.gene057882.t1 MTR_4g114850 44.030 268 148 2 22 288 24 290 1.39e-67 215
MS.gene057882.t1 MTR_4g114850 44.030 268 148 2 22 288 24 290 1.59e-67 215
MS.gene057882.t1 MTR_4g114850 44.030 268 148 2 22 288 24 290 1.70e-67 215
MS.gene057882.t1 MTR_4g083500 44.649 271 145 5 23 291 43 310 1.97e-67 219
MS.gene057882.t1 MTR_1g022160 40.000 270 159 3 24 291 24 292 3.61e-67 217
MS.gene057882.t1 MTR_1g022160 40.000 270 159 3 24 291 24 292 3.70e-67 217
MS.gene057882.t1 MTR_1g022160 40.000 270 159 3 24 291 24 292 5.43e-67 217
MS.gene057882.t1 MTR_5g078200 44.231 260 141 2 35 291 1 259 3.73e-65 215
MS.gene057882.t1 MTR_4g076470 39.931 288 160 6 11 297 10 285 3.57e-64 205
MS.gene057882.t1 MTR_4g076570 41.697 271 153 3 21 291 19 284 1.84e-63 204
MS.gene057882.t1 MTR_4g076440 40.278 288 159 6 11 297 10 285 1.86e-62 201
MS.gene057882.t1 MTR_2g034480 41.379 290 159 6 6 291 9 291 2.65e-62 201
MS.gene057882.t1 MTR_1g052430 42.599 277 148 6 24 297 32 300 2.77e-62 202
MS.gene057882.t1 MTR_3g083580 36.014 286 176 3 11 290 10 294 4.48e-62 204
MS.gene057882.t1 MTR_4g076430 40.138 289 158 7 11 297 10 285 2.69e-61 197
MS.gene057882.t1 MTR_8g085720 38.889 270 161 2 24 290 26 294 5.13e-61 201
MS.gene057882.t1 MTR_4g076490 40.000 275 152 6 24 297 97 359 1.82e-59 196
MS.gene057882.t1 MTR_4g076255 39.716 282 158 6 16 297 17 286 2.30e-58 190
MS.gene057882.t1 MTR_7g026340 40.647 278 156 6 16 291 15 285 2.60e-58 193
MS.gene057882.t1 MTR_2g034470 38.112 286 169 5 14 297 21 300 2.24e-57 188
MS.gene057882.t1 MTR_7g081350 36.237 287 175 5 5 291 8 286 4.24e-57 187
MS.gene057882.t1 MTR_4g076500 37.868 272 160 3 21 292 75 337 7.44e-57 188
MS.gene057882.t1 MTR_8g012400 42.667 225 126 3 77 299 2 225 1.64e-55 184
MS.gene057882.t1 MTR_2g034440 37.857 280 166 5 14 291 13 286 4.24e-55 182
MS.gene057882.t1 MTR_2g034470 37.918 269 160 4 31 297 2 265 8.82e-54 178
MS.gene057882.t1 MTR_7g053500 42.593 216 121 3 77 290 2 216 1.67e-53 179
MS.gene057882.t1 MTR_8g102340 38.433 268 130 3 24 291 28 260 1.88e-53 181
MS.gene057882.t1 MTR_7g081370 35.889 287 170 7 1 282 1 278 4.41e-52 174
MS.gene057882.t1 MTR_3g116510 48.765 162 82 1 130 291 1 161 1.01e-46 160
MS.gene057882.t1 MTR_4g112025 33.110 299 184 10 2 291 8 299 6.28e-44 157
MS.gene057882.t1 MTR_1g053320 34.576 295 177 8 6 291 12 299 3.19e-43 154
MS.gene057882.t1 MTR_2g027560 35.897 273 164 7 24 291 28 294 3.28e-43 154
MS.gene057882.t1 MTR_8g092070 31.206 282 175 7 5 270 4 282 8.26e-38 141
MS.gene057882.t1 MTR_4g124440 31.419 296 187 9 4 289 3 292 8.49e-38 140
MS.gene057882.t1 MTR_1g010120 34.532 278 166 9 4 272 6 276 2.90e-37 138
MS.gene057882.t1 MTR_6g032820 31.724 290 182 8 4 284 6 288 3.62e-37 137
MS.gene057882.t1 MTR_5g081720 32.685 257 166 5 40 293 53 305 1.49e-36 134
MS.gene057882.t1 MTR_7g085390 33.074 257 159 8 24 272 26 277 1.80e-36 136
MS.gene057882.t1 MTR_3g031770 35.573 253 156 6 24 272 27 276 3.58e-36 135
MS.gene057882.t1 MTR_3g095050 36.328 256 143 11 24 269 34 279 7.23e-35 132
MS.gene057882.t1 MTR_3g108600 32.028 281 163 7 9 285 16 272 5.34e-33 126
MS.gene057882.t1 MTR_4g134280 34.615 182 110 6 112 291 3 177 1.03e-22 97.1
MS.gene057882.t1 MTR_4g036775 41.176 85 48 1 6 88 2 86 1.07e-12 66.6
MS.gene057882.t1 MTR_4g036680 43.056 72 41 0 17 88 15 86 1.69e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057882.t1 AT2G05790 73.801 271 71 0 20 290 21 291 3.59e-148 424
MS.gene057882.t1 AT5G55180 64.207 271 97 0 20 290 23 293 6.63e-123 360
MS.gene057882.t1 AT5G55180 64.207 271 97 0 20 290 23 293 7.35e-123 360
MS.gene057882.t1 AT4G26830 62.847 288 104 2 5 290 4 290 1.89e-120 353
MS.gene057882.t1 AT4G26830 65.441 272 93 1 19 290 24 294 8.53e-120 352
MS.gene057882.t1 AT1G30080 48.327 269 136 3 23 290 35 301 3.37e-77 240
MS.gene057882.t1 AT1G30080 48.327 269 136 3 23 290 35 301 4.46e-77 241
MS.gene057882.t1 AT4G18340 45.993 287 144 5 11 289 15 298 3.13e-75 235
MS.gene057882.t1 AT4G18340 45.993 287 144 5 11 289 15 298 4.31e-75 235
MS.gene057882.t1 AT1G32860 43.866 269 148 3 23 290 28 294 5.05e-74 233
MS.gene057882.t1 AT4G14080 42.857 280 158 2 21 299 39 317 9.67e-74 234
MS.gene057882.t1 AT2G16230 43.494 269 150 2 24 291 25 292 3.09e-73 234
MS.gene057882.t1 AT5G42100 47.232 271 138 4 23 290 26 294 8.06e-73 230
MS.gene057882.t1 AT5G42100 47.232 271 138 4 23 290 26 294 9.37e-73 230
MS.gene057882.t1 AT2G27500 44.403 268 147 2 24 290 29 295 1.70e-72 228
MS.gene057882.t1 AT1G11820 40.959 271 156 3 24 291 44 313 3.39e-72 228
MS.gene057882.t1 AT2G27500 44.403 268 147 2 24 290 29 295 4.86e-72 227
MS.gene057882.t1 AT5G42720 42.612 291 158 5 10 293 8 296 6.97e-72 228
MS.gene057882.t1 AT1G11820 40.959 271 156 3 24 291 44 313 4.05e-71 227
MS.gene057882.t1 AT1G66250 43.542 271 149 2 24 291 32 301 4.32e-71 228
MS.gene057882.t1 AT3G23770 40.989 283 162 2 21 299 34 315 5.68e-71 227
MS.gene057882.t1 AT3G15800 44.981 269 145 3 22 288 41 308 8.06e-71 224
MS.gene057882.t1 AT1G11820 40.959 271 156 3 24 291 44 313 1.01e-70 227
MS.gene057882.t1 AT2G01630 44.649 271 146 2 24 291 23 292 1.76e-70 223
MS.gene057882.t1 AT3G07320 42.705 281 158 3 21 299 22 301 3.11e-70 224
MS.gene057882.t1 AT2G01630 44.649 271 146 2 24 291 23 292 7.22e-70 223
MS.gene057882.t1 AT2G01630 44.649 271 146 2 24 291 23 292 2.99e-69 223
MS.gene057882.t1 AT2G26600 43.284 268 150 2 22 288 32 298 6.25e-69 219
MS.gene057882.t1 AT5G24318 39.446 289 168 4 3 289 9 292 8.66e-69 218
MS.gene057882.t1 AT5G56590 40.293 273 160 3 21 291 23 294 2.10e-68 221
MS.gene057882.t1 AT5G24318 39.446 289 168 4 3 289 9 292 4.31e-68 219
MS.gene057882.t1 AT5G24318 39.446 289 168 4 3 289 9 292 6.59e-68 218
MS.gene057882.t1 AT4G34480 40.753 292 168 4 3 291 4 293 1.32e-67 215
MS.gene057882.t1 AT4G29360 41.522 289 168 1 3 291 6 293 6.17e-67 218
MS.gene057882.t1 AT4G34480 40.753 292 168 4 3 291 4 293 2.69e-66 216
MS.gene057882.t1 AT4G29360 40.134 299 168 2 3 291 6 303 4.61e-65 213
MS.gene057882.t1 AT3G13560 38.129 278 168 3 16 290 18 294 1.93e-63 208
MS.gene057882.t1 AT3G13560 38.129 278 168 3 16 290 18 294 1.93e-63 208
MS.gene057882.t1 AT3G13560 38.129 278 168 3 16 290 18 294 1.93e-63 208
MS.gene057882.t1 AT3G13560 38.129 278 168 3 16 290 18 294 1.93e-63 208
MS.gene057882.t1 AT3G13560 38.129 278 168 3 16 290 18 294 1.93e-63 208
MS.gene057882.t1 AT3G61810 39.927 273 158 3 24 291 55 326 3.64e-62 201
MS.gene057882.t1 AT3G46570 40.625 288 160 8 9 291 12 293 2.49e-58 191
MS.gene057882.t1 AT3G57270 39.350 277 162 4 21 297 24 294 2.91e-58 190
MS.gene057882.t1 AT4G16260 39.649 285 163 7 4 288 5 280 5.08e-58 190
MS.gene057882.t1 AT3G57260 40.433 277 155 5 21 297 29 295 3.73e-54 179
MS.gene057882.t1 AT3G57260 40.433 277 155 5 21 297 45 311 5.62e-54 179
MS.gene057882.t1 AT5G20390 38.603 272 158 6 21 291 30 293 6.65e-54 179
MS.gene057882.t1 AT4G16260 40.310 258 146 6 31 288 2 251 1.24e-53 177
MS.gene057882.t1 AT2G27500 41.935 217 124 2 75 290 2 217 1.68e-51 172
MS.gene057882.t1 AT1G77780 37.324 284 164 8 13 290 7 282 3.01e-50 170
MS.gene057882.t1 AT3G57240 37.993 279 161 7 19 297 30 296 1.01e-48 166
MS.gene057882.t1 AT3G55430 37.193 285 163 10 11 290 18 291 2.37e-48 167
MS.gene057882.t1 AT5G20340 37.770 278 163 7 16 291 34 303 1.48e-47 163
MS.gene057882.t1 AT1G77790 36.655 281 170 6 14 293 19 292 1.49e-47 162
MS.gene057882.t1 AT3G24330 36.000 300 175 10 2 292 16 307 2.00e-47 166
MS.gene057882.t1 AT1G33220 35.786 299 167 8 6 291 8 294 3.35e-47 161
MS.gene057882.t1 AT5G20330 36.949 295 169 7 6 291 8 294 8.79e-47 160
MS.gene057882.t1 AT2G26600 40.909 198 115 2 92 288 8 204 3.19e-46 157
MS.gene057882.t1 AT4G34480 47.253 182 92 3 112 291 3 182 1.81e-42 150
MS.gene057882.t1 AT2G39640 33.904 292 179 9 4 293 12 291 7.22e-42 152
MS.gene057882.t1 AT5G58480 37.786 262 154 7 24 279 26 284 2.29e-41 149
MS.gene057882.t1 AT5G24318 43.312 157 85 1 133 289 1 153 9.43e-41 144
MS.gene057882.t1 AT3G55780 31.086 267 176 6 37 297 48 312 7.66e-40 143
MS.gene057882.t1 AT5G18220 34.559 272 163 8 24 289 30 292 1.57e-39 144
MS.gene057882.t1 AT4G17180 33.798 287 174 9 9 291 10 284 1.99e-39 144
MS.gene057882.t1 AT2G19440 32.967 273 169 9 6 270 3 269 4.20e-38 140
MS.gene057882.t1 AT2G19440 32.967 273 169 9 6 270 3 269 4.20e-38 140
MS.gene057882.t1 AT5G58090 33.094 278 171 9 21 291 19 288 2.47e-36 135
MS.gene057882.t1 AT1G64760 33.333 255 156 8 24 270 25 273 4.98e-36 135
MS.gene057882.t1 AT1G64760 33.333 255 156 8 24 270 25 273 4.98e-36 135
MS.gene057882.t1 AT5G20560 30.797 276 183 6 17 291 27 295 3.60e-35 130
MS.gene057882.t1 AT3G04010 32.069 290 178 10 10 289 17 297 6.55e-35 132
MS.gene057882.t1 AT4G31140 32.770 296 186 10 4 293 7 295 9.42e-35 131
MS.gene057882.t1 AT5G64790 30.943 265 173 7 11 269 15 275 7.69e-33 126
MS.gene057882.t1 AT5G20870 31.560 282 180 7 2 272 8 287 1.29e-32 126

Find 53 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCGACAGTGAGTTTGATTT+TGG 0.204771 5.3:+69327635 None:intergenic
TAATCTTCATAATGATATTA+AGG 0.220512 5.3:-69327412 MS.gene057882:CDS
AACTCTTACCCTTCTTCTTC+CGG 0.243510 5.3:-69327357 MS.gene057882:CDS
CCCATTGCTCTTTCTGCTCT+TGG 0.246418 5.3:-69327381 MS.gene057882:CDS
AACGTCGTCGTTTCGGAAAC+GGG 0.333129 5.3:-69327072 MS.gene057882:CDS
AGGTATTTCGTCGAGTTGTT+TGG 0.344374 5.3:+69327477 None:intergenic
TGATATAAACGTCGTCGTTT+CGG 0.347375 5.3:-69327079 MS.gene057882:CDS
TTGCTGCTGCAAAGAGTTGT+TGG 0.357391 5.3:+69327604 None:intergenic
AACAGCAATGGCTTCTATAA+TGG 0.358498 5.3:+69327521 None:intergenic
ACTCTTACCCTTCTTCTTCC+GGG 0.359608 5.3:-69327356 MS.gene057882:CDS
AAACGTCGTCGTTTCGGAAA+CGG 0.380698 5.3:-69327073 MS.gene057882:CDS
GTCAGGTGGATGCGGGTAAC+GGG 0.390822 5.3:-69327173 MS.gene057882:CDS
GGTCAGGTGGATGCGGGTAA+CGG 0.402489 5.3:-69327174 MS.gene057882:CDS
TCAGTTCTACGTGCATTATC+CGG 0.456312 5.3:-69327660 MS.gene057882:CDS
TTCATTGCTCTCACAAATGC+TGG 0.461229 5.3:-69327798 MS.gene057882:CDS
GGTAAATGTGAATTTCCAAC+TGG 0.463834 5.3:-69326027 MS.gene057882:CDS
CTTCTATAATGGTGTTCGGT+TGG 0.480558 5.3:+69327532 None:intergenic
ATTATAGAAGCCATTGCTGT+TGG 0.480731 5.3:-69327519 MS.gene057882:CDS
GGCAACATAGGCATAAACTA+TGG 0.481308 5.3:-69327777 MS.gene057882:CDS
CGGAATGACCCGGAAGAAGA+AGG 0.481349 5.3:+69327348 None:intergenic
TGCTGCAAAGAGTTGTTGGT+TGG 0.483787 5.3:+69327608 None:intergenic
ATGGCTTCTATAATGGTGTT+CGG 0.494847 5.3:+69327528 None:intergenic
CTCACAAATGCTGGCAACAT+AGG 0.503433 5.3:-69327789 MS.gene057882:CDS
CGTCGATTTGAGCATCGAAA+AGG 0.510151 5.3:+69327136 None:intergenic
CCAAGAGCAGAAAGAGCAAT+GGG 0.510634 5.3:+69327381 None:intergenic
AACCCGGGTCAGGTGGATGC+GGG 0.512461 5.3:-69327180 MS.gene057882:CDS
GAACCCGGGTCAGGTGGATG+CGG 0.523730 5.3:-69327181 MS.gene057882:CDS
AACTTCATTTCCAACAGCAA+TGG 0.527788 5.3:+69327509 None:intergenic
GCTGCAAAGAGTTGTTGGTT+GGG 0.533883 5.3:+69327609 None:intergenic
GATAATGCACGTAGAACTGA+TGG 0.534281 5.3:+69327663 None:intergenic
AAAACAGGTTGGATTAACTC+GGG 0.535304 5.3:+69327324 None:intergenic
TCACGAAGAAAATCAAGCAT+GGG 0.551379 5.3:+69327297 None:intergenic
TTCACGAAGAAAATCAAGCA+TGG 0.551918 5.3:+69327296 None:intergenic
TCGTGAGAACCCGGGTCAGG+TGG 0.554914 5.3:-69327187 MS.gene057882:CDS
TTACCCGCATCCACCTGACC+CGG 0.556109 5.3:+69327177 None:intergenic
GTTAACCATGAGGTAAGATC+CGG 0.556543 5.3:+69327272 None:intergenic
GAAGATGAGGAAGATGAAGA+AGG 0.557301 5.3:+69327818 None:intergenic
ACCAAGAGCAGAAAGAGCAA+TGG 0.563188 5.3:+69327380 None:intergenic
TCATTACCATCACCTTTCGA+AGG 0.568639 5.3:+69327045 None:intergenic
CAGGTTGGATTAACTCGGGT+CGG 0.574158 5.3:+69327328 None:intergenic
GAAACGGGTTGGCCTTCGAA+AGG 0.578195 5.3:-69327057 MS.gene057882:CDS
GGTTGGCCTTCGAAAGGTGA+TGG 0.601053 5.3:-69327051 MS.gene057882:CDS
TTGTGAGAGCAATGAAGATG+AGG 0.606268 5.3:+69327805 None:intergenic
TCGTCGTTTCGGAAACGGGT+TGG 0.611751 5.3:-69327068 MS.gene057882:CDS
GGAATGACCCGGAAGAAGAA+GGG 0.615815 5.3:+69327349 None:intergenic
TACCCGCATCCACCTGACCC+GGG 0.629795 5.3:+69327178 None:intergenic
TGAAACCGGATCTTACCTCA+TGG 0.630499 5.3:-69327277 MS.gene057882:CDS
TAACTCGGGTCGGAATGACC+CGG 0.634539 5.3:+69327338 None:intergenic
GAAAGGTGATGGTAATGAAG+TGG 0.650157 5.3:-69327040 MS.gene057882:CDS
AAAGGTGATGGTAATGAAGT+GGG 0.661071 5.3:-69327039 MS.gene057882:CDS
ATAATGCACGTAGAACTGAT+GGG 0.681139 5.3:+69327664 None:intergenic
ACGGGTAAACGTTAACCATG+AGG 0.688527 5.3:+69327262 None:intergenic
GTTCAACTCCTAAAATCACA+AGG 0.702475 5.3:-69327717 MS.gene057882:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATCTTCATAATGATATTA+AGG - chr5.3:69326448-69326467 MS.gene057882:intron 15.0%
!! GTAATTATAGAATACTTACT+AGG + chr5.3:69327394-69327413 None:intergenic 20.0%
!! TAAAATGAGCTTATAGTATA+TGG - chr5.3:69327472-69327491 MS.gene057882:CDS 20.0%
!! TTCTAAATTAGTCAAAGATT+GGG - chr5.3:69327696-69327715 MS.gene057882:CDS 20.0%
!!! AAAAAGTGCGAATAAAAAAA+CGG + chr5.3:69326942-69326961 None:intergenic 20.0%
!!! AATCTTTTATGAAATTGACA+TGG - chr5.3:69327531-69327550 MS.gene057882:CDS 20.0%
!!! ATGCTTTTATTATTGTTACT+AGG - chr5.3:69327639-69327658 MS.gene057882:CDS 20.0%
!!! TGCTTTTATTATTGTTACTA+GGG - chr5.3:69327640-69327659 MS.gene057882:CDS 20.0%
! TTTAATGAAAATCAAAAGCC+CGG - chr5.3:69326959-69326978 MS.gene057882:intron 25.0%
! TTTGATTCATAAGCGAAAAA+CGG + chr5.3:69326620-69326639 None:intergenic 25.0%
!! GTTCTAAATTAGTCAAAGAT+TGG - chr5.3:69327695-69327714 MS.gene057882:CDS 25.0%
!! TGATCTGTAAATGTTTTTCA+TGG + chr5.3:69326417-69326436 None:intergenic 25.0%
!! TTAGGGTTAATTTTACTACA+GGG - chr5.3:69327508-69327527 MS.gene057882:CDS 25.0%
!!! TTTAGGGTTAATTTTACTAC+AGG - chr5.3:69327507-69327526 MS.gene057882:CDS 25.0%
ACAAGAGTAATTGGAATTGT+TGG - chr5.3:69327663-69327682 MS.gene057882:CDS 30.0%
CAATAGTTATTATCAGAAGC+AGG - chr5.3:69327312-69327331 MS.gene057882:CDS 30.0%
CTAACAAAAACAATGCGAAA+AGG + chr5.3:69327416-69327435 None:intergenic 30.0%
CTACTTCAGAAAGAAACTTT+GGG - chr5.3:69326984-69327003 MS.gene057882:intron 30.0%
GTAAGATCTTGACATCAAAA+GGG - chr5.3:69326888-69326907 MS.gene057882:intron 30.0%
TATAGCGTAGTTTAGATTTG+TGG - chr5.3:69327446-69327465 MS.gene057882:CDS 30.0%
TGTAAACTTTCTTCTCATCA+GGG + chr5.3:69327018-69327037 None:intergenic 30.0%
TTAATGAAAATCAAAAGCCC+GGG - chr5.3:69326960-69326979 MS.gene057882:intron 30.0%
TTGATTCATAAGCGAAAAAC+GGG + chr5.3:69326619-69326638 None:intergenic 30.0%
TTGTAAACTTTCTTCTCATC+AGG + chr5.3:69327019-69327038 None:intergenic 30.0%
! AAAAACAGGTTGGATTAACT+CGG + chr5.3:69326540-69326559 None:intergenic 30.0%
! CTTTTGATGTCAAGATCTTA+CGG + chr5.3:69326889-69326908 None:intergenic 30.0%
! TCTGTAAATGTTTTTCATGG+CGG + chr5.3:69326414-69326433 None:intergenic 30.0%
! TGTAAATTTTGTGCAGAGTA+TGG - chr5.3:69327812-69327831 MS.gene057882:CDS 30.0%
!!! AGCTTGTAGCTTAGTTTTAT+CGG + chr5.3:69327185-69327204 None:intergenic 30.0%
!!! GTGTTTTGATCCTAATACAT+TGG - chr5.3:69327267-69327286 MS.gene057882:CDS 30.0%
AACAGCAATGGCTTCTATAA+TGG + chr5.3:69326342-69326361 None:intergenic 35.0%
AACTTCATTTCCAACAGCAA+TGG + chr5.3:69326354-69326373 None:intergenic 35.0%
ATAATGCACGTAGAACTGAT+GGG + chr5.3:69326199-69326218 None:intergenic 35.0%
ATGGCTTCTATAATGGTGTT+CGG + chr5.3:69326335-69326354 None:intergenic 35.0%
ATTATAGAAGCCATTGCTGT+TGG - chr5.3:69326341-69326360 MS.gene057882:intron 35.0%
CCTACTTCAGAAAGAAACTT+TGG - chr5.3:69326983-69327002 MS.gene057882:intron 35.0%
CGTAAGATCTTGACATCAAA+AGG - chr5.3:69326887-69326906 MS.gene057882:intron 35.0%
GCTATCAATGTACACACTAT+AGG + chr5.3:69327613-69327632 None:intergenic 35.0%
GGTAAATGTGAATTTCCAAC+TGG - chr5.3:69327833-69327852 MS.gene057882:CDS 35.0%
GTTCAACTCCTAAAATCACA+AGG - chr5.3:69326143-69326162 MS.gene057882:intron 35.0%
TCAAGCATGGGTTTAAAAAC+AGG + chr5.3:69326554-69326573 None:intergenic 35.0%
TCACGAAGAAAATCAAGCAT+GGG + chr5.3:69326566-69326585 None:intergenic 35.0%
TGACATGGCGTAACTTTATT+GGG - chr5.3:69327546-69327565 MS.gene057882:CDS 35.0%
TGATATAAACGTCGTCGTTT+CGG - chr5.3:69326781-69326800 MS.gene057882:intron 35.0%
TGTATTAGGATCAAAACACG+TGG + chr5.3:69327266-69327285 None:intergenic 35.0%
TTACAATGTTCCTTTAACCG+TGG - chr5.3:69327033-69327052 MS.gene057882:CDS 35.0%
TTACTAGGGACAAGAGTAAT+TGG - chr5.3:69327654-69327673 MS.gene057882:CDS 35.0%
TTCACGAAGAAAATCAAGCA+TGG + chr5.3:69326567-69326586 None:intergenic 35.0%
TTGACATGGCGTAACTTTAT+TGG - chr5.3:69327545-69327564 MS.gene057882:CDS 35.0%
! AAAACAGGTTGGATTAACTC+GGG + chr5.3:69326539-69326558 None:intergenic 35.0%
! AAAGGTGATGGTAATGAAGT+GGG - chr5.3:69326821-69326840 MS.gene057882:intron 35.0%
! ATAGTGTGTACATTGATAGC+TGG - chr5.3:69327613-69327632 MS.gene057882:CDS 35.0%
! ATGGTAAGCACGTGATTTTA+GGG - chr5.3:69327491-69327510 MS.gene057882:CDS 35.0%
! CTGTAAATGTTTTTCATGGC+GGG + chr5.3:69326413-69326432 None:intergenic 35.0%
! CTTGATTTTCTTCGTGAAAC+CGG - chr5.3:69326569-69326588 MS.gene057882:intron 35.0%
! GGGTTATACCTTGTGATTTT+AGG + chr5.3:69326154-69326173 None:intergenic 35.0%
! TATGGTAAGCACGTGATTTT+AGG - chr5.3:69327490-69327509 MS.gene057882:CDS 35.0%
! TCAATCTTTTGCACTCTCAT+GGG - chr5.3:69326283-69326302 MS.gene057882:intron 35.0%
! TGGGTAGAGTGTACTATTTT+TGG - chr5.3:69327565-69327584 MS.gene057882:CDS 35.0%
!! ATCGACAGTGAGTTTGATTT+TGG + chr5.3:69326228-69326247 None:intergenic 35.0%
!! CCAAAGTTTCTTTCTGAAGT+AGG + chr5.3:69326986-69327005 None:intergenic 35.0%
!! GATTGGGTTTTGTGTTACAT+TGG - chr5.3:69327712-69327731 MS.gene057882:CDS 35.0%
!! GTAATTTTTCAAAGCCTCCA+CGG + chr5.3:69327053-69327072 None:intergenic 35.0%
!! TGAACTACTTTTGTTGCTGA+GGG + chr5.3:69326128-69326147 None:intergenic 35.0%
!! TGTGTCGTAGATTTTGACAT+GGG + chr5.3:69326174-69326193 None:intergenic 35.0%
!! TTGAACTACTTTTGTTGCTG+AGG + chr5.3:69326129-69326148 None:intergenic 35.0%
!!! AGTGAGTTTGATTTTGGAAC+CGG + chr5.3:69326222-69326241 None:intergenic 35.0%
AAAAACGGTGAGTTCTGCTT+TGG + chr5.3:69326927-69326946 None:intergenic 40.0%
AAAACGGTGAGTTCTGCTTT+GGG + chr5.3:69326926-69326945 None:intergenic 40.0%
AACTAAGCTACAAGCTGCAT+TGG - chr5.3:69327189-69327208 MS.gene057882:CDS 40.0%
AACTCTTACCCTTCTTCTTC+CGG - chr5.3:69326503-69326522 MS.gene057882:intron 40.0%
CTTCTATAATGGTGTTCGGT+TGG + chr5.3:69326331-69326350 None:intergenic 40.0%
GAAGATGAGGAAGATGAAGA+AGG + chr5.3:69326045-69326064 None:intergenic 40.0%
GATAATGCACGTAGAACTGA+TGG + chr5.3:69326200-69326219 None:intergenic 40.0%
GCATGGGTTTAAAAACAGGT+TGG + chr5.3:69326550-69326569 None:intergenic 40.0%
GCATGTGCAACCAATGTATT+AGG + chr5.3:69327280-69327299 None:intergenic 40.0%
GGAGCATGTTGTATGATGTA+GGG - chr5.3:69327586-69327605 MS.gene057882:CDS 40.0%
GGCAACATAGGCATAAACTA+TGG - chr5.3:69326083-69326102 MS.gene057882:intron 40.0%
GTTAACCATGAGGTAAGATC+CGG + chr5.3:69326591-69326610 None:intergenic 40.0%
TCAGTTCTACGTGCATTATC+CGG - chr5.3:69326200-69326219 MS.gene057882:intron 40.0%
TCATTACCATCACCTTTCGA+AGG + chr5.3:69326818-69326837 None:intergenic 40.0%
TGGAGCATGTTGTATGATGT+AGG - chr5.3:69327585-69327604 MS.gene057882:CDS 40.0%
TTCATTGCTCTCACAAATGC+TGG - chr5.3:69326062-69326081 MS.gene057882:intron 40.0%
TTGTGAGAGCAATGAAGATG+AGG + chr5.3:69326058-69326077 None:intergenic 40.0%
! AGGTATTTCGTCGAGTTGTT+TGG + chr5.3:69326386-69326405 None:intergenic 40.0%
! ATTGGATTTTGCTTGTGGTG+AGG - chr5.3:69327207-69327226 MS.gene057882:CDS 40.0%
! CTCAATCTTTTGCACTCTCA+TGG - chr5.3:69326282-69326301 MS.gene057882:intron 40.0%
! GAAAGGTGATGGTAATGAAG+TGG - chr5.3:69326820-69326839 MS.gene057882:intron 40.0%
! TTGGATTTTGCTTGTGGTGA+GGG - chr5.3:69327208-69327227 MS.gene057882:CDS 40.0%
!! CTGTGTCGTAGATTTTGACA+TGG + chr5.3:69326175-69326194 None:intergenic 40.0%
!!! AGCTTAGTTTTATCGGCGTA+AGG + chr5.3:69327178-69327197 None:intergenic 40.0%
!!! CACGTGGCATTGTTTTGAAT+CGG + chr5.3:69327250-69327269 None:intergenic 40.0%
!!! TGGCATTGTTTTGAATCGGA+CGG + chr5.3:69327246-69327265 None:intergenic 40.0%
AAACGTCGTCGTTTCGGAAA+CGG - chr5.3:69326787-69326806 MS.gene057882:intron 45.0%
ACCAAGAGCAGAAAGAGCAA+TGG + chr5.3:69326483-69326502 None:intergenic 45.0%
ACGGGTAAACGTTAACCATG+AGG + chr5.3:69326601-69326620 None:intergenic 45.0%
ACTCTTACCCTTCTTCTTCC+GGG - chr5.3:69326504-69326523 MS.gene057882:intron 45.0%
CAATGTTCCTTTAACCGTGG+AGG - chr5.3:69327036-69327055 MS.gene057882:CDS 45.0%
CCAAGAGCAGAAAGAGCAAT+GGG + chr5.3:69326482-69326501 None:intergenic 45.0%
CTCACAAATGCTGGCAACAT+AGG - chr5.3:69326071-69326090 MS.gene057882:intron 45.0%
TGAAACCGGATCTTACCTCA+TGG - chr5.3:69326583-69326602 MS.gene057882:intron 45.0%
TGCTGCAAAGAGTTGTTGGT+TGG + chr5.3:69326255-69326274 None:intergenic 45.0%
TTCAAAGCCTCCACGGTTAA+AGG + chr5.3:69327046-69327065 None:intergenic 45.0%
TTGCTGCTGCAAAGAGTTGT+TGG + chr5.3:69326259-69326278 None:intergenic 45.0%
! GCTGCAAAGAGTTGTTGGTT+GGG + chr5.3:69326254-69326273 None:intergenic 45.0%
! GCTGCATTGGATTTTGCTTG+TGG - chr5.3:69327202-69327221 MS.gene057882:CDS 45.0%
!! CGTCGATTTGAGCATCGAAA+AGG + chr5.3:69326727-69326746 None:intergenic 45.0%
!! CTACTTTTGTTGCTGAGGGT+AGG + chr5.3:69326124-69326143 None:intergenic 45.0%
!! GTTTCTTTCTGAAGTAGGCC+CGG + chr5.3:69326981-69327000 None:intergenic 45.0%
!! TGCTCTTTTTCGTGAGAACC+CGG - chr5.3:69326664-69326683 MS.gene057882:intron 45.0%
!! TTTCTTTCTGAAGTAGGCCC+GGG + chr5.3:69326980-69326999 None:intergenic 45.0%
!!! GCTGGTGGAAGTTGCTATTT+TGG - chr5.3:69327340-69327359 MS.gene057882:CDS 45.0%
!!! GGTGGAAGTTGCTATTTTGG+TGG - chr5.3:69327343-69327362 MS.gene057882:CDS 45.0%
AACGTCGTCGTTTCGGAAAC+GGG - chr5.3:69326788-69326807 MS.gene057882:intron 50.0%
CATCAAAAGGGACCCCACTT+AGG - chr5.3:69326900-69326919 MS.gene057882:intron 50.0%
CCCATTGCTCTTTCTGCTCT+TGG - chr5.3:69326479-69326498 MS.gene057882:intron 50.0%
GGAATGACCCGGAAGAAGAA+GGG + chr5.3:69326514-69326533 None:intergenic 50.0%
TTACCATGATGACCCAGCAC+CGG - chr5.3:69327069-69327088 MS.gene057882:CDS 50.0%
! AGTTCTGCTTTGGGCCTAAG+TGG + chr5.3:69326917-69326936 None:intergenic 50.0%
! CAGGTTGGATTAACTCGGGT+CGG + chr5.3:69326535-69326554 None:intergenic 50.0%
! GATTTTGCTTGTGGTGAGGG+TGG - chr5.3:69327211-69327230 MS.gene057882:CDS 50.0%
! GTTCTGCTTTGGGCCTAAGT+GGG + chr5.3:69326916-69326935 None:intergenic 50.0%
! TTCTGCTTTGGGCCTAAGTG+GGG + chr5.3:69326915-69326934 None:intergenic 50.0%
!! GCTCTTTTTCGTGAGAACCC+GGG - chr5.3:69326665-69326684 MS.gene057882:intron 50.0%
!! GGTGTTTCGAAGAGTACCAC+CGG - chr5.3:69327130-69327149 MS.gene057882:CDS 50.0%
!! GTGTTTCGAAGAGTACCACC+GGG - chr5.3:69327131-69327150 MS.gene057882:CDS 50.0%
!! TTTTGGTGGTACGTCGTACG+TGG - chr5.3:69327357-69327376 MS.gene057882:CDS 50.0%
!!! TGTTTTTCATGGCGGGTACG+AGG + chr5.3:69326406-69326425 None:intergenic 50.0%
CGGAATGACCCGGAAGAAGA+AGG + chr5.3:69326515-69326534 None:intergenic 55.0%
TAACTCGGGTCGGAATGACC+CGG + chr5.3:69326525-69326544 None:intergenic 55.0%
TATCAGAAGCAGGCACGTGC+TGG - chr5.3:69327322-69327341 MS.gene057882:CDS 55.0%
TCGTCGTTTCGGAAACGGGT+TGG - chr5.3:69326792-69326811 MS.gene057882:intron 55.0%
TGCAACACACCACGTGTTCC+CGG + chr5.3:69327152-69327171 None:intergenic 55.0%
! TTTTCGTGAGAACCCGGGTC+AGG - chr5.3:69326670-69326689 MS.gene057882:intron 55.0%
!! GAAACGGGTTGGCCTTCGAA+AGG - chr5.3:69326803-69326822 MS.gene057882:intron 55.0%
!! GGTTGGCCTTCGAAAGGTGA+TGG - chr5.3:69326809-69326828 MS.gene057882:intron 55.0%
AAACCGGTGAAGCCGGTGCT+GGG + chr5.3:69327084-69327103 None:intergenic 60.0%
AACACACCACGTGTTCCCGG+TGG + chr5.3:69327149-69327168 None:intergenic 60.0%
GGTCAGGTGGATGCGGGTAA+CGG - chr5.3:69326686-69326705 MS.gene057882:intron 60.0%
GTCAGGTGGATGCGGGTAAC+GGG - chr5.3:69326687-69326706 MS.gene057882:intron 60.0%
TTACCCGCATCCACCTGACC+CGG + chr5.3:69326686-69326705 None:intergenic 60.0%
! AAGCCGGTGCTGGGTCATCA+TGG + chr5.3:69327075-69327094 None:intergenic 60.0%
! AGAGTACCACCGGGAACACG+TGG - chr5.3:69327140-69327159 MS.gene057882:CDS 60.0%
AACCCGGGTCAGGTGGATGC+GGG - chr5.3:69326680-69326699 MS.gene057882:intron 65.0%
ACCACCACTACCACCGCCAC+CGG + chr5.3:69327107-69327126 None:intergenic 65.0%
CAGAAGCAGGCACGTGCTGG+TGG - chr5.3:69327325-69327344 MS.gene057882:CDS 65.0%
CCACCGGAAACCGGTGAAGC+CGG + chr5.3:69327091-69327110 None:intergenic 65.0%
CTACCACCGCCACCGGAAAC+CGG + chr5.3:69327100-69327119 None:intergenic 65.0%
GAAACCGGTGAAGCCGGTGC+TGG + chr5.3:69327085-69327104 None:intergenic 65.0%
GAACCCGGGTCAGGTGGATG+CGG - chr5.3:69326679-69326698 MS.gene057882:intron 65.0%
TACCCGCATCCACCTGACCC+GGG + chr5.3:69326685-69326704 None:intergenic 65.0%
TCGTGAGAACCCGGGTCAGG+TGG - chr5.3:69326673-69326692 MS.gene057882:intron 65.0%
! GTTTCCGGTGGCGGTGGTAG+TGG - chr5.3:69327100-69327119 MS.gene057882:CDS 65.0%
!! GCACCGGCTTCACCGGTTTC+CGG - chr5.3:69327085-69327104 MS.gene057882:CDS 65.0%
!! GCTTCACCGGTTTCCGGTGG+CGG - chr5.3:69327091-69327110 MS.gene057882:CDS 65.0%
!! TGACCCAGCACCGGCTTCAC+CGG - chr5.3:69327078-69327097 MS.gene057882:CDS 65.0%
!! CCGGCTTCACCGGTTTCCGG+TGG - chr5.3:69327088-69327107 MS.gene057882:CDS 70.0%
!! GGCGGTGGTAGTGGTGGTGG+TGG - chr5.3:69327109-69327128 MS.gene057882:CDS 70.0%
!! GGTGGCGGTGGTAGTGGTGG+TGG - chr5.3:69327106-69327125 MS.gene057882:CDS 70.0%
!! TCACCGGTTTCCGGTGGCGG+TGG - chr5.3:69327094-69327113 MS.gene057882:CDS 70.0%
!! TCCGGTGGCGGTGGTAGTGG+TGG - chr5.3:69327103-69327122 MS.gene057882:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 69326020 69327862 69326020 ID=MS.gene057882
chr5.3 mRNA 69326020 69327862 69326020 ID=MS.gene057882.t1;Parent=MS.gene057882
chr5.3 exon 69326995 69327862 69326995 ID=MS.gene057882.t1.exon1;Parent=MS.gene057882.t1
chr5.3 CDS 69326995 69327862 69326995 ID=cds.MS.gene057882.t1;Parent=MS.gene057882.t1
chr5.3 exon 69326020 69326054 69326020 ID=MS.gene057882.t1.exon2;Parent=MS.gene057882.t1
chr5.3 CDS 69326020 69326054 69326020 ID=cds.MS.gene057882.t1;Parent=MS.gene057882.t1
Gene Sequence

>MS.gene057882

ATGCATAACTTCACTCTATTCACCTTCTTCATCTTCCTCATCTTCATTGCTCTCACAAATGCTGGCAACATAGGCATAAACTATGGTCGTATAGCAAACAACCTACCCTCAGCAACAAAAGTAGTTCAACTCCTAAAATCACAAGGTATAACCCATGTCAAAATCTACGACACAGACCCATCAGTTCTACGTGCATTATCCGGTTCCAAAATCAAACTCACTGTCGATCTACCCAACCAACAACTCTTTGCAGCAGCAAAGTCTCAATCTTTTGCACTCTCATGGGTCGAAAGAAACATAGTTGCTTTCCAACCGAACACCATTATAGAAGCCATTGCTGTTGGAAATGAAGTTTTTGTTGATCCAAACAACTCGACGAAATACCTCGTACCCGCCATGAAAAACATTTACAGATCACTACAAAAACATAATCTTCATAATGATATTAAGGTTTCTTCACCCATTGCTCTTTCTGCTCTTGGTAACTCTTACCCTTCTTCTTCCGGGTCATTCCGACCCGAGTTAATCCAACCTGTTTTTAAACCCATGCTTGATTTTCTTCGTGAAACCGGATCTTACCTCATGGTTAACGTTTACCCGTTTTTCGCTTATGAATCAAACGCTAATGTTATCTCATTAGACTATGCTCTTTTTCGTGAGAACCCGGGTCAGGTGGATGCGGGTAACGGGTTGAGATACTTGAACCTTTTCGATGCTCAAATCGACGCTGTTTTCGCAGCACTATCTCGTTTGAAATACGATGATATAAACGTCGTCGTTTCGGAAACGGGTTGGCCTTCGAAAGGTGATGGTAATGAAGTGGGTGCGAGTGTAGAGAATGCAGCTGCATACAACGCTAATCTCGTCCGTAAGATCTTGACATCAAAAGGGACCCCACTTAGGCCCAAAGCAGAACTCACCGTTTTTTTATTCGCACTTTTTAATGAAAATCAAAAGCCCGGGCCTACTTCAGAAAGAAACTTTGGGCTTTTTTACCCTGATGAGAAGAAAGTTTACAATGTTCCTTTAACCGTGGAGGCTTTGAAAAATTACCATGATGACCCAGCACCGGCTTCACCGGTTTCCGGTGGCGGTGGTAGTGGTGGTGGTGGTGTTTCGAAGAGTACCACCGGGAACACGTGGTGTGTTGCAAATCCTTACGCCGATAAAACTAAGCTACAAGCTGCATTGGATTTTGCTTGTGGTGAGGGTGGTGCTGATTGCCGTCCGATTCAAAACAATGCCACGTGTTTTGATCCTAATACATTGGTTGCACATGCTTCATTTGCGTTCAATAGTTATTATCAGAAGCAGGCACGTGCTGGTGGAAGTTGCTATTTTGGTGGTACGTCGTACGTGGTCACTCAAGAGCCTAGTAAGTATTCTATAATTACCTTTTCGCATTGTTTTTGTTAGATTCTTGTAATATAGCGTAGTTTAGATTTGTGGTGCTAAAATGAGCTTATAGTATATGGTAAGCACGTGATTTTAGGGTTAATTTTACTACAGGGAATCTTTTATGAAATTGACATGGCGTAACTTTATTGGGTAGAGTGTACTATTTTTGGAGCATGTTGTATGATGTAGGGTCCTATAGTGTGTACATTGATAGCTGGAAAATGCTTTTATTATTGTTACTAGGGACAAGAGTAATTGGAATTGTTGGACTGTACTAGTTCTAAATTAGTCAAAGATTGGGTTTTGTGTTACATTGGATTCTAGTATTATTTAACTGTAGTACTGTTTGTTCTTTTATTGATTTAATTTCAATTCAATTTTTTGTATTAATGTGTGTAAATTTTGTGCAGAGTATGGTAAATGTGAATTTCCAACTGGATACTAG
Protein sequence

>MS.gene057882.t1

MHNFTLFTFFIFLIFIALTNAGNIGINYGRIANNLPSATKVVQLLKSQGITHVKIYDTDPSVLRALSGSKIKLTVDLPNQQLFAAAKSQSFALSWVERNIVAFQPNTIIEAIAVGNEVFVDPNNSTKYLVPAMKNIYRSLQKHNLHNDIKVSSPIALSALGNSYPSSSGSFRPELIQPVFKPMLDFLRETGSYLMVNVYPFFAYESNANVISLDYALFRENPGQVDAGNGLRYLNLFDAQIDAVFAALSRLKYDDINVVVSETGWPSKGDGNEVGASVENAAAYNANLVQYGKCEFPTGY