AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058531

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058531.t1 MTR_7g078330 93.007 429 22 2 27 448 26 453 0.0 815
MS.gene058531.t1 MTR_4g063800 37.731 432 243 6 39 448 12 439 5.96e-86 276
MS.gene058531.t1 MTR_1g094215 35.731 431 257 5 30 448 9 431 5.94e-72 239
MS.gene058531.t1 MTR_7g059405 33.643 431 257 8 43 448 21 447 1.66e-60 208
MS.gene058531.t1 MTR_4g485520 33.793 435 232 14 54 448 41 459 7.99e-47 171
MS.gene058531.t1 MTR_2g018010 31.059 425 259 13 53 444 55 478 9.77e-41 155
MS.gene058531.t1 MTR_1g094215 37.549 253 141 4 208 448 1 248 9.43e-39 145
MS.gene058531.t1 MTR_5g083030 26.695 472 269 10 43 448 31 491 2.90e-38 147
MS.gene058531.t1 MTR_4g485520 43.455 191 93 3 272 448 102 291 1.44e-37 143
MS.gene058531.t1 MTR_4g028960 30.357 392 236 10 86 448 93 476 1.64e-33 134
MS.gene058531.t1 MTR_4g107010 23.953 430 285 11 52 449 52 471 3.33e-25 109
MS.gene058531.t1 MTR_3g085610 27.607 326 180 9 67 343 37 355 6.26e-23 102
MS.gene058531.t1 MTR_3g078160 25.490 408 266 11 52 430 46 444 1.18e-21 98.2
MS.gene058531.t1 MTR_3g078160 25.495 404 271 10 52 430 46 444 1.27e-21 97.8
MS.gene058531.t1 MTR_3g078160 25.490 408 266 11 52 430 46 444 1.84e-21 97.8
MS.gene058531.t1 MTR_7g116600 31.667 180 108 5 276 442 79 256 1.75e-20 94.0
MS.gene058531.t1 MTR_7g053260 34.254 181 105 6 272 440 75 253 4.61e-20 92.8
MS.gene058531.t1 MTR_8g077205 26.686 341 180 12 56 343 27 350 1.32e-19 92.0
MS.gene058531.t1 MTR_8g080280 23.251 486 259 13 67 445 38 516 2.49e-19 91.3
MS.gene058531.t1 MTR_3g466220 32.335 167 88 4 187 342 134 286 4.50e-18 87.4
MS.gene058531.t1 MTR_3g466220 46.237 93 48 2 358 449 529 620 1.19e-13 73.6
MS.gene058531.t1 MTR_3g466220 32.335 167 88 4 187 342 155 307 4.82e-18 87.4
MS.gene058531.t1 MTR_3g466220 46.237 93 48 2 358 449 550 641 1.17e-13 73.9
MS.gene058531.t1 MTR_1g079450 33.708 178 104 5 272 437 60 235 5.78e-18 86.3
MS.gene058531.t1 MTR_1g069845 25.633 316 195 7 39 335 14 308 3.60e-15 78.6
MS.gene058531.t1 MTR_1g069845 44.828 87 46 2 364 449 443 528 3.39e-12 68.9
MS.gene058531.t1 MTR_1g069845 25.000 320 192 8 39 335 18 312 3.98e-15 78.2
MS.gene058531.t1 MTR_1g069845 44.828 87 46 2 364 449 447 532 3.65e-12 68.9
MS.gene058531.t1 MTR_5g034440 24.490 392 230 13 46 394 48 416 5.33e-14 74.7
MS.gene058531.t1 MTR_1g093965 32.738 168 77 7 288 448 1 139 6.30e-14 70.9
MS.gene058531.t1 MTR_8g077205 27.586 203 98 7 184 343 42 238 8.24e-14 73.9
MS.gene058531.t1 MTR_5g020570 33.929 112 58 2 267 371 265 367 2.87e-13 72.4
MS.gene058531.t1 MTR_7g097020 25.753 299 195 9 54 343 36 316 3.19e-13 72.4
MS.gene058531.t1 MTR_7g097020 39.474 114 59 3 345 449 458 570 4.10e-11 65.9
MS.gene058531.t1 MTR_5g020570 33.929 112 58 2 267 371 280 382 4.02e-13 72.0
MS.gene058531.t1 MTR_1g069845 31.944 144 73 3 203 335 62 191 4.04e-13 71.6
MS.gene058531.t1 MTR_1g069845 44.828 87 46 2 364 449 326 411 2.86e-12 69.3
MS.gene058531.t1 MTR_4g085720 38.235 102 47 3 275 368 14 107 8.22e-12 67.0
MS.gene058531.t1 MTR_5g048050 29.710 138 89 2 273 402 23 160 8.77e-12 67.0
MS.gene058531.t1 MTR_6g071340 28.986 138 90 2 273 402 3 140 1.43e-11 66.6
MS.gene058531.t1 MTR_1g056870 29.070 172 97 5 279 433 11 174 2.27e-11 65.9
MS.gene058531.t1 MTR_2g026130 37.500 96 59 1 353 448 167 261 2.54e-11 65.9
MS.gene058531.t1 MTR_1g018180 39.796 98 57 2 354 450 53 149 2.66e-11 65.1
MS.gene058531.t1 MTR_1g056840 29.240 171 98 4 279 433 11 174 3.66e-11 65.1
MS.gene058531.t1 MTR_1g056880 29.240 171 98 4 279 433 11 174 5.27e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058531.t1 AT5G42340 51.522 427 185 6 32 448 53 467 1.06e-146 433
MS.gene058531.t1 AT3G46510 39.720 428 238 6 39 448 15 440 2.83e-87 280
MS.gene058531.t1 AT3G54850 33.023 430 258 8 33 448 20 433 5.98e-67 226
MS.gene058531.t1 AT2G28830 35.027 374 215 9 102 448 1 373 2.83e-53 188
MS.gene058531.t1 AT1G23030 33.180 434 262 10 27 448 2 419 1.96e-50 181
MS.gene058531.t1 AT1G71020 31.155 459 249 12 27 448 2 430 3.26e-45 166
MS.gene058531.t1 AT1G29340 27.897 466 286 13 26 443 26 489 1.34e-37 146
MS.gene058531.t1 AT1G71020 39.691 194 103 2 269 448 89 282 2.72e-34 134
MS.gene058531.t1 AT5G01830 27.596 366 235 9 100 448 104 456 7.12e-30 123
MS.gene058531.t1 AT1G27910 24.187 492 259 14 63 448 32 515 5.18e-26 111
MS.gene058531.t1 AT1G67530 27.492 331 191 8 55 343 24 347 2.80e-25 109
MS.gene058531.t1 AT1G67530 27.492 331 191 8 55 343 24 347 2.80e-25 109
MS.gene058531.t1 AT1G67530 27.492 331 191 8 55 343 24 347 2.80e-25 109
MS.gene058531.t1 AT1G67530 27.492 331 191 8 55 343 24 347 3.72e-25 108
MS.gene058531.t1 AT3G07360 37.017 181 95 7 276 439 75 253 5.92e-24 104
MS.gene058531.t1 AT1G24330 26.087 322 186 9 67 343 36 350 1.12e-21 98.6
MS.gene058531.t1 AT1G24330 26.087 322 186 9 67 343 36 350 1.12e-21 98.6
MS.gene058531.t1 AT3G19380 32.821 195 102 5 262 436 1 186 4.56e-19 89.4
MS.gene058531.t1 AT1G49780 34.545 165 85 3 262 410 1 158 1.03e-18 88.6
MS.gene058531.t1 AT2G23140 45.122 82 38 1 269 343 224 305 8.85e-17 83.6
MS.gene058531.t1 AT2G23140 43.750 96 52 2 355 449 537 631 2.48e-13 72.8
MS.gene058531.t1 AT2G23140 45.122 82 38 1 269 343 224 305 8.85e-17 83.6
MS.gene058531.t1 AT2G23140 43.750 96 52 2 355 449 537 631 2.48e-13 72.8
MS.gene058531.t1 AT2G23140 45.122 82 38 1 269 343 227 308 8.95e-17 83.6
MS.gene058531.t1 AT2G23140 43.750 96 52 2 355 449 540 634 2.47e-13 72.8
MS.gene058531.t1 AT2G23140 45.122 82 38 1 269 343 35 116 1.35e-16 82.8
MS.gene058531.t1 AT2G23140 43.750 96 52 2 355 449 348 442 2.57e-13 72.4
MS.gene058531.t1 AT2G23140 45.122 82 38 1 269 343 35 116 1.35e-16 82.8
MS.gene058531.t1 AT2G23140 43.750 96 52 2 355 449 348 442 2.57e-13 72.4
MS.gene058531.t1 AT1G60190 25.581 387 257 12 80 444 82 459 3.07e-16 81.6
MS.gene058531.t1 AT1G10560 24.668 377 243 10 100 443 103 471 1.82e-15 79.3
MS.gene058531.t1 AT5G67340 34.074 135 73 3 275 399 240 368 3.58e-14 75.5
MS.gene058531.t1 AT5G67340 40.171 117 66 4 336 449 396 511 4.88e-14 74.7
MS.gene058531.t1 AT5G67340 34.074 135 73 3 275 399 240 368 3.58e-14 75.5
MS.gene058531.t1 AT5G67340 40.171 117 66 4 336 449 396 511 4.88e-14 74.7
MS.gene058531.t1 AT5G18320 31.667 180 106 5 275 440 72 248 4.07e-14 74.7
MS.gene058531.t1 AT3G54790 30.337 178 95 5 186 351 123 283 1.80e-12 70.1
MS.gene058531.t1 AT3G54790 30.337 178 95 5 186 351 159 319 1.84e-12 70.1
MS.gene058531.t1 AT3G54790 30.337 178 95 5 186 351 159 319 1.84e-12 70.1
MS.gene058531.t1 AT2G35930 32.667 150 86 5 275 415 12 155 2.63e-12 68.9
MS.gene058531.t1 AT4G36550 23.793 290 188 8 60 340 26 291 6.98e-11 65.1

Find 82 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATAATTTCAAGTGTTAT+TGG 0.211508 7.4:+27900574 None:intergenic
CAAACACATGTTGCCTCTTA+TGG 0.212487 7.4:-27902069 MS.gene058531:CDS
CCAAACATGGAATTTCTTCT+TGG 0.230264 7.4:+27899829 None:intergenic
CCAAGAAGAAATTCCATGTT+TGG 0.295569 7.4:-27899829 MS.gene058531:CDS
GCTTGGAAGAAGCACTTCTT+TGG 0.328604 7.4:-27900620 MS.gene058531:CDS
CTTCTTCCAAGCATCTTATT+AGG 0.336614 7.4:+27900631 None:intergenic
TTCCTCGAAACGGCGTTGTT+TGG 0.340973 7.4:-27902019 MS.gene058531:CDS
TTCAGCAATTAAATTTCTTA+AGG 0.357358 7.4:+27899923 None:intergenic
CGAAGAAAAGCGCATCTCTA+AGG 0.359752 7.4:+27901988 None:intergenic
GGTGTGCCTTGTGATGAATT+AGG 0.367708 7.4:-27901669 MS.gene058531:CDS
GTGTGCCTTGTGATGAATTA+GGG 0.381169 7.4:-27901668 MS.gene058531:CDS
TCCGAAGACAAGGCAGCCTT+TGG 0.394785 7.4:-27899973 MS.gene058531:CDS
ATTGAATCGGTGGTTCAATT+CGG 0.409503 7.4:-27902143 MS.gene058531:CDS
AACCAAACAACGCCGTTTCG+AGG 0.411594 7.4:+27902017 None:intergenic
AAATTTCTTAAGGCACAGTT+TGG 0.411871 7.4:+27899933 None:intergenic
TGGCCACTTGCAATAATAAC+AGG 0.415456 7.4:+27900547 None:intergenic
TCCAAAGGCTGCCTTGTCTT+CGG 0.419783 7.4:+27899972 None:intergenic
ACAACGCCGTTTCGAGGAAC+AGG 0.427957 7.4:+27902023 None:intergenic
CCAACACTTCTATGTATAGC+TGG 0.433109 7.4:+27899564 None:intergenic
AAACCTTGTTAGAGAAAGAA+AGG 0.438472 7.4:-27900761 MS.gene058531:CDS
GCCATGCCTAATAAGATGCT+TGG 0.439094 7.4:-27900637 MS.gene058531:CDS
TTCTGATGAAGTAAAAGAAC+AGG 0.452149 7.4:-27901643 MS.gene058531:intron
TAAGAAATTTAATTGCTGAA+TGG 0.452536 7.4:-27899920 MS.gene058531:CDS
TTCCCTTTCTTTCTCTAACA+AGG 0.457758 7.4:+27900758 None:intergenic
CAATGGTGTGGTGGTGGTGG+AGG 0.458616 7.4:-27902180 MS.gene058531:CDS
ATGCAATGGTGTGGTGGTGG+TGG 0.460589 7.4:-27902183 MS.gene058531:CDS
CCACCGATTCAATGAGTCCT+TGG 0.474954 7.4:+27902153 None:intergenic
GAAACGGCGTTGTTTGGTTG+AGG 0.484598 7.4:-27902013 MS.gene058531:CDS
CCTTTCGATAATGGCACTAT+CGG 0.487902 7.4:+27900846 None:intergenic
GAGGTAGAAACATTGGCTGT+TGG 0.489533 7.4:-27900784 MS.gene058531:CDS
AGGCTGCCTTGTCTTCGGAC+AGG 0.490475 7.4:+27899977 None:intergenic
TATGATAGGATTTGTACTAA+TGG 0.494085 7.4:+27899687 None:intergenic
AGCTAATGCACGTGCAACTC+AGG 0.495077 7.4:-27900954 MS.gene058531:CDS
TGTTATTATTGCAAGTGGCC+AGG 0.495390 7.4:-27900545 MS.gene058531:intron
TCCAAGCATCTTATTAGGCA+TGG 0.497480 7.4:+27900636 None:intergenic
TCACGAAGGTCTTCCATAAG+AGG 0.499928 7.4:+27902056 None:intergenic
ACCTTCTTAACAGATTCAAA+AGG 0.513046 7.4:-27900705 MS.gene058531:CDS
AACAGATTCAAAAGGACTGC+AGG 0.520736 7.4:-27900697 MS.gene058531:CDS
AGAGCTAGCAATGGATATGA+TGG 0.525717 7.4:-27900899 MS.gene058531:CDS
ATTCAAAAGGACTGCAGGCA+TGG 0.529095 7.4:-27900692 MS.gene058531:CDS
CATCATCCTGTTCCTCGAAA+CGG 0.531583 7.4:-27902029 MS.gene058531:CDS
GATCCAAGGACTCATTGAAT+CGG 0.548729 7.4:-27902156 MS.gene058531:CDS
CAACTTAGAACATCAAAGAA+AGG 0.556469 7.4:-27899787 MS.gene058531:CDS
GTTCTAAGTTGATCGACGAT+AGG 0.558574 7.4:+27899799 None:intergenic
TTAGGCATGGGAAGGTGAGA+TGG 0.558729 7.4:-27901742 MS.gene058531:intron
AGAATGCAATGGTGTGGTGG+TGG 0.564838 7.4:-27902186 MS.gene058531:CDS
GATCCTGTTATTATTGCAAG+TGG 0.569659 7.4:-27900550 MS.gene058531:CDS
GGATCTTGAGGTAGAAACAT+TGG 0.571196 7.4:-27900791 MS.gene058531:CDS
AACCTTGTTAGAGAAAGAAA+GGG 0.572058 7.4:-27900760 MS.gene058531:CDS
AATGGAAGGAACAGAAGAGA+GGG 0.575343 7.4:-27902261 MS.gene058531:CDS
GGTTATGATCAAATTTCAGA+AGG 0.576249 7.4:-27901721 MS.gene058531:CDS
TTGTTGAAATTGTGTAGCCA+AGG 0.579205 7.4:-27901957 MS.gene058531:CDS
GATAGGATTTGTACTAATGG+TGG 0.579251 7.4:+27899690 None:intergenic
TCACAGTGTTGAGGATCTTG+AGG 0.580007 7.4:-27900803 MS.gene058531:CDS
ACGTGCAACTCAGGAGGGCA+AGG 0.580025 7.4:-27900945 MS.gene058531:CDS
AAGGCACACCATCAAAAGCT+TGG 0.581211 7.4:+27901682 None:intergenic
AACAGGTGACTTTCAATGGA+AGG 0.586665 7.4:-27902275 None:intergenic
ACAAAACCTGTCCGAAGACA+AGG 0.586908 7.4:-27899983 MS.gene058531:CDS
GGAAGGAACAGAAGAGAGGG+AGG 0.596520 7.4:-27902258 MS.gene058531:CDS
TTCGAGGAACAGGATGATGA+AGG 0.600018 7.4:+27902033 None:intergenic
GTTGGAGCTTCACAGTGTTG+AGG 0.602329 7.4:-27900812 MS.gene058531:CDS
TAATGCACGTGCAACTCAGG+AGG 0.604700 7.4:-27900951 MS.gene058531:CDS
CCAAGGACTCATTGAATCGG+TGG 0.611282 7.4:-27902153 MS.gene058531:CDS
TGTGTTTGAAACGACGAGCG+AGG 0.614739 7.4:+27902084 None:intergenic
AAAGGGAAAGCATAGAGAAG+TGG 0.617041 7.4:-27900020 MS.gene058531:intron
CCGATAGTGCCATTATCGAA+AGG 0.622407 7.4:-27900846 MS.gene058531:CDS
CAATGGAAGGAACAGAAGAG+AGG 0.623216 7.4:-27902262 MS.gene058531:CDS
AGAAGAATGCAATGGTGTGG+TGG 0.641470 7.4:-27902189 MS.gene058531:CDS
ACAAGACATAGAGCTAGCAA+TGG 0.643295 7.4:-27900908 MS.gene058531:CDS
AATGCACGTGCAACTCAGGA+GGG 0.649570 7.4:-27900950 MS.gene058531:CDS
GTGGTGGTGGAGGAGATCCA+AGG 0.649832 7.4:-27902170 MS.gene058531:CDS
AGAACCAGAATCAAGCAACC+CGG 0.656438 7.4:-27902228 MS.gene058531:CDS
ATCATCATCAAAGATAACAT+CGG 0.668304 7.4:+27900663 None:intergenic
AATGAAGAAGAAGAATGCAA+TGG 0.671502 7.4:-27902197 MS.gene058531:CDS
GCTAATTGAAGATGTTCCAA+AGG 0.672940 7.4:+27899957 None:intergenic
GAAATCCCTAATTCATCACA+AGG 0.673692 7.4:+27901663 None:intergenic
CAGGATGATGAAGGTCACGA+AGG 0.693076 7.4:+27902042 None:intergenic
ATTGGACACAGAAACTCATG+AGG 0.705096 7.4:+27900592 None:intergenic
CTTAGAACATCAAAGAAAGG+CGG 0.713277 7.4:-27899784 MS.gene058531:CDS
GCTAGCAATGGATATGATGG+TGG 0.719121 7.4:-27900896 MS.gene058531:CDS
AGAAGAAGAATGCAATGGTG+TGG 0.735055 7.4:-27902192 MS.gene058531:CDS
GCGAATACAGAAGAACACAA+AGG 0.740376 7.4:-27902121 MS.gene058531:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAACTTATATCAAAAGA+AGG - chr7.4:27902237-27902256 MS.gene058531:CDS 15.0%
!! TCAATATATAATTATGTGTA+TGG - chr7.4:27900286-27900305 MS.gene058531:intron 15.0%
!! TGAAATAATGTTATATACTT+TGG - chr7.4:27900840-27900859 MS.gene058531:CDS 15.0%
!! TTCGTATTATAAATTTGATT+CGG - chr7.4:27901477-27901496 MS.gene058531:intron 15.0%
!! ACTAGTCATATTTATGAAAA+TGG - chr7.4:27901375-27901394 MS.gene058531:intron 20.0%
!! CTATATAATAATGCTAACAA+AGG - chr7.4:27901733-27901752 MS.gene058531:CDS 20.0%
!! TAAGAAATTTAATTGCTGAA+TGG - chr7.4:27901911-27901930 MS.gene058531:intron 20.0%
!! TATTGAATAAATGGTGTATT+TGG + chr7.4:27900584-27900603 None:intergenic 20.0%
!! TGCTTTAATAATTACACATT+TGG - chr7.4:27900624-27900643 MS.gene058531:CDS 20.0%
!! TTCAGCAATTAAATTTCTTA+AGG + chr7.4:27901911-27901930 None:intergenic 20.0%
!! TTGTAGGTTTATTGAATAAA+TGG + chr7.4:27900593-27900612 None:intergenic 20.0%
!!! AAAAGTGTAATTATTAAGAG+CGG + chr7.4:27900490-27900509 None:intergenic 20.0%
!!! AATTGAATAACTGAAGTATT+TGG - chr7.4:27900545-27900564 MS.gene058531:intron 20.0%
!!! ATTGAATAACTGAAGTATTT+TGG - chr7.4:27900546-27900565 MS.gene058531:CDS 20.0%
!!! GATTCTTTTATTTTTGAATC+TGG + chr7.4:27902171-27902190 None:intergenic 20.0%
!!! TCTATGATTTCTCTTTTTTA+AGG - chr7.4:27899987-27900006 MS.gene058531:CDS 20.0%
!!! TGATTATGTTTTGATGAATA+GGG - chr7.4:27900037-27900056 MS.gene058531:intron 20.0%
!!! TTGATTATGTTTTGATGAAT+AGG - chr7.4:27900036-27900055 MS.gene058531:intron 20.0%
! ATCATCATCAAAGATAACAT+CGG + chr7.4:27901171-27901190 None:intergenic 25.0%
! ATTTCTCACTCTTAATCTAA+CGG - chr7.4:27901611-27901630 MS.gene058531:intron 25.0%
! TATGATAGGATTTGTACTAA+TGG + chr7.4:27902147-27902166 None:intergenic 25.0%
! TATTGAATAACTGGTGTATT+TGG + chr7.4:27900720-27900739 None:intergenic 25.0%
! TTGTAGGTTTATTGAATAAC+TGG + chr7.4:27900729-27900748 None:intergenic 25.0%
!! GACATAATTTCAAGTGTTAT+TGG + chr7.4:27901260-27901279 None:intergenic 25.0%
!! GCTCTTAATAATTACACTTT+TGG - chr7.4:27900489-27900508 MS.gene058531:intron 25.0%
!! TTGTTGCTTATAATAGTGAT+CGG - chr7.4:27900756-27900775 MS.gene058531:CDS 25.0%
!! TTTTTGAATCTGGATATGAT+AGG + chr7.4:27902161-27902180 None:intergenic 25.0%
!!! AACTTTCTTTTGATAACATG+CGG + chr7.4:27902083-27902102 None:intergenic 25.0%
!!! AAGCAACAAATCATTTTTGT+AGG + chr7.4:27900609-27900628 None:intergenic 25.0%
!!! AAGTTTGTTTGTTATTTACG+AGG + chr7.4:27899912-27899931 None:intergenic 25.0%
!!! TCTTTTTTTTCTTGCGTTTT+AGG - chr7.4:27900071-27900090 MS.gene058531:intron 25.0%
AAACCTTGTTAGAGAAAGAA+AGG - chr7.4:27901070-27901089 MS.gene058531:intron 30.0%
AAATTTCTTAAGGCACAGTT+TGG + chr7.4:27901901-27901920 None:intergenic 30.0%
AACCTTGTTAGAGAAAGAAA+GGG - chr7.4:27901071-27901090 MS.gene058531:intron 30.0%
AATGAAGAAGAAGAATGCAA+TGG - chr7.4:27899634-27899653 MS.gene058531:CDS 30.0%
AATGCATGTATTTGTGAATG+CGG + chr7.4:27900354-27900373 None:intergenic 30.0%
ACCTTCTTAACAGATTCAAA+AGG - chr7.4:27901126-27901145 MS.gene058531:intron 30.0%
CAACTTAGAACATCAAAGAA+AGG - chr7.4:27902044-27902063 MS.gene058531:CDS 30.0%
GGTTATGATCAAATTTCAGA+AGG - chr7.4:27900110-27900129 MS.gene058531:intron 30.0%
TCCATCAAAGACATATGAAA+GGG - chr7.4:27901794-27901813 MS.gene058531:intron 30.0%
TTCCATCAAAGACATATGAA+AGG - chr7.4:27901793-27901812 MS.gene058531:intron 30.0%
TTCTGATGAAGTAAAAGAAC+AGG - chr7.4:27900188-27900207 MS.gene058531:intron 30.0%
TTTCACATTTAAGCCTAAGT+TGG - chr7.4:27901346-27901365 MS.gene058531:intron 30.0%
! AAATTAAGCCAAGCTTTTGA+TGG - chr7.4:27900141-27900160 MS.gene058531:intron 30.0%
! ATCTGGAATGAAGAAAACTT+AGG - chr7.4:27901702-27901721 MS.gene058531:CDS 30.0%
! TCCTTTTGAATCTGTTAAGA+AGG + chr7.4:27901130-27901149 None:intergenic 30.0%
! TCTTCTTCATTCTCATTTTC+CGG + chr7.4:27899625-27899644 None:intergenic 30.0%
! TGACATGAAGAACAGATTTT+TGG + chr7.4:27901961-27901980 None:intergenic 30.0%
! TTATGAAAATGGATTCGATG+CGG - chr7.4:27901386-27901405 MS.gene058531:intron 30.0%
!! TGGAATTTCTTCTTGGTTTT+CGG + chr7.4:27901998-27902017 None:intergenic 30.0%
!! TTCTGAAATCCAATCATCTA+CGG - chr7.4:27901553-27901572 MS.gene058531:intron 30.0%
!!! ATGGAACCCTTATTTTATTC+CGG - chr7.4:27900305-27900324 MS.gene058531:intron 30.0%
ACCTTGCCGGAATAAAATAA+GGG + chr7.4:27900314-27900333 None:intergenic 35.0%
ACTACTAGCAACATAGTGAT+CGG + chr7.4:27900795-27900814 None:intergenic 35.0%
ACTGTTGCATACATATTGGA+CGG - chr7.4:27901441-27901460 MS.gene058531:intron 35.0%
ATTGAATCGGTGGTTCAATT+CGG - chr7.4:27899688-27899707 MS.gene058531:CDS 35.0%
CACAAATACATGCATTCACA+TGG - chr7.4:27900358-27900377 MS.gene058531:intron 35.0%
CCAAACATGGAATTTCTTCT+TGG + chr7.4:27902005-27902024 None:intergenic 35.0%
CCAAGAAGAAATTCCATGTT+TGG - chr7.4:27902002-27902021 MS.gene058531:CDS 35.0%
CTTAGAACATCAAAGAAAGG+CGG - chr7.4:27902047-27902066 MS.gene058531:CDS 35.0%
CTTCATTCCAGATTCTCTAA+AGG + chr7.4:27901695-27901714 None:intergenic 35.0%
CTTCTTCCAAGCATCTTATT+AGG + chr7.4:27901203-27901222 None:intergenic 35.0%
GAAATCCCTAATTCATCACA+AGG + chr7.4:27900171-27900190 None:intergenic 35.0%
GAACTTAATTCGTGATTACC+TGG + chr7.4:27901307-27901326 None:intergenic 35.0%
GACTAGTATCTTTCCAACTT+AGG + chr7.4:27901362-27901381 None:intergenic 35.0%
GATAGGATTTGTACTAATGG+TGG + chr7.4:27902144-27902163 None:intergenic 35.0%
GATCCTGTTATTATTGCAAG+TGG - chr7.4:27901281-27901300 MS.gene058531:intron 35.0%
GCTAATTGAAGATGTTCCAA+AGG + chr7.4:27901877-27901896 None:intergenic 35.0%
TCCAATCTTTATCCAACAGT+CGG - chr7.4:27901520-27901539 MS.gene058531:intron 35.0%
TCCCTTTCATATGTCTTTGA+TGG + chr7.4:27901798-27901817 None:intergenic 35.0%
TGCTTATAATAGTGATCGGA+TGG - chr7.4:27900760-27900779 MS.gene058531:CDS 35.0%
TTCCCTTTCTTTCTCTAACA+AGG + chr7.4:27901076-27901095 None:intergenic 35.0%
TTGTTGAAATTGTGTAGCCA+AGG - chr7.4:27899874-27899893 MS.gene058531:CDS 35.0%
! CTTCTTCATTCTCATTTTCC+GGG + chr7.4:27899624-27899643 None:intergenic 35.0%
! GATAGGTTTTCAACCAAACA+TGG + chr7.4:27902018-27902037 None:intergenic 35.0%
! TTTTGCAAGCCTTTCGATAA+TGG + chr7.4:27900997-27901016 None:intergenic 35.0%
!! AGAATTTTAGTCGCCGAAAA+CGG - chr7.4:27902111-27902130 MS.gene058531:CDS 35.0%
!! ATCTGCTTGTTTTCTACATC+TGG + chr7.4:27900397-27900416 None:intergenic 35.0%
!! ATTTCTTCTTGGTTTTCGGT+TGG + chr7.4:27901994-27902013 None:intergenic 35.0%
!! ATTTTGGCCTTTAGAGAATC+TGG - chr7.4:27901685-27901704 MS.gene058531:CDS 35.0%
!! TTTTCTTGCGTTTTAGGCAT+GGG - chr7.4:27900077-27900096 MS.gene058531:intron 35.0%
!! TTTTTCTTGCGTTTTAGGCA+TGG - chr7.4:27900076-27900095 MS.gene058531:intron 35.0%
AACAGATTCAAAAGGACTGC+AGG - chr7.4:27901134-27901153 MS.gene058531:intron 40.0%
ACAAGACATAGAGCTAGCAA+TGG - chr7.4:27900923-27900942 MS.gene058531:CDS 40.0%
AGAAGAAGAATGCAATGGTG+TGG - chr7.4:27899639-27899658 MS.gene058531:CDS 40.0%
AGAGCTAGCAATGGATATGA+TGG - chr7.4:27900932-27900951 MS.gene058531:CDS 40.0%
AGGCCAAAATCCACAAACTT+CGG + chr7.4:27901675-27901694 None:intergenic 40.0%
ATTGGACACAGAAACTCATG+AGG + chr7.4:27901242-27901261 None:intergenic 40.0%
CAAACACATGTTGCCTCTTA+TGG - chr7.4:27899762-27899781 MS.gene058531:CDS 40.0%
CACCTTGCCGGAATAAAATA+AGG + chr7.4:27900315-27900334 None:intergenic 40.0%
CATTCGTGCAATTTGCACAT+TGG + chr7.4:27900436-27900455 None:intergenic 40.0%
CGAAAGGCTTGCAAAAAAGT+TGG - chr7.4:27901001-27901020 MS.gene058531:intron 40.0%
CTCGACTGTTGCATACATAT+TGG - chr7.4:27901437-27901456 MS.gene058531:intron 40.0%
GATCCAAGGACTCATTGAAT+CGG - chr7.4:27899675-27899694 MS.gene058531:CDS 40.0%
GCGAATACAGAAGAACACAA+AGG - chr7.4:27899710-27899729 MS.gene058531:CDS 40.0%
GGATCTTGAGGTAGAAACAT+TGG - chr7.4:27901040-27901059 MS.gene058531:intron 40.0%
GTGTGCCTTGTGATGAATTA+GGG - chr7.4:27900163-27900182 MS.gene058531:intron 40.0%
TCCAAGCATCTTATTAGGCA+TGG + chr7.4:27901198-27901217 None:intergenic 40.0%
TCCGACTGTTGGATAAAGAT+TGG + chr7.4:27901524-27901543 None:intergenic 40.0%
TGGCCACTTGCAATAATAAC+AGG + chr7.4:27901287-27901306 None:intergenic 40.0%
TGTTATTATTGCAAGTGGCC+AGG - chr7.4:27901286-27901305 MS.gene058531:intron 40.0%
TTTGTGAATGCGGTGTATTG+CGG + chr7.4:27900344-27900363 None:intergenic 40.0%
! AAAGGGAAAGCATAGAGAAG+TGG - chr7.4:27901811-27901830 MS.gene058531:intron 40.0%
! ATTTGCACATTGGTGGTTGT+TGG + chr7.4:27900426-27900445 None:intergenic 40.0%
! CCTTTCGATAATGGCACTAT+CGG + chr7.4:27900988-27901007 None:intergenic 40.0%
!! GTTCTAAGTTGATCGACGAT+AGG + chr7.4:27902035-27902054 None:intergenic 40.0%
!! TTCGGACAGGTTTTGTGATT+GGG + chr7.4:27901844-27901863 None:intergenic 40.0%
!!! ACCCTTATTTTATTCCGGCA+AGG - chr7.4:27900310-27900329 MS.gene058531:intron 40.0%
AAGGCACACCATCAAAAGCT+TGG + chr7.4:27900152-27900171 None:intergenic 45.0%
ACAAAACCTGTCCGAAGACA+AGG - chr7.4:27901848-27901867 MS.gene058531:intron 45.0%
ACATCATAGTGCAGCTGTAG+AGG - chr7.4:27901648-27901667 MS.gene058531:CDS 45.0%
AGAACCAGAATCAAGCAACC+CGG - chr7.4:27899603-27899622 MS.gene058531:CDS 45.0%
AGAAGAATGCAATGGTGTGG+TGG - chr7.4:27899642-27899661 MS.gene058531:CDS 45.0%
ATTCAAAAGGACTGCAGGCA+TGG - chr7.4:27901139-27901158 MS.gene058531:intron 45.0%
CATCATCCTGTTCCTCGAAA+CGG - chr7.4:27899802-27899821 MS.gene058531:CDS 45.0%
GCATTCACATGGTCAATCGT+TGG - chr7.4:27900369-27900388 MS.gene058531:intron 45.0%
GCCATGCCTAATAAGATGCT+TGG - chr7.4:27901194-27901213 MS.gene058531:intron 45.0%
GCTAGCAATGGATATGATGG+TGG - chr7.4:27900935-27900954 MS.gene058531:CDS 45.0%
GGTGTGCCTTGTGATGAATT+AGG - chr7.4:27900162-27900181 MS.gene058531:intron 45.0%
GTGCAAATTGCACGAATGCA+CGG - chr7.4:27900438-27900457 MS.gene058531:intron 45.0%
TCACGAAGGTCTTCCATAAG+AGG + chr7.4:27899778-27899797 None:intergenic 45.0%
TCGTGCAATTTGCACATTGG+TGG + chr7.4:27900433-27900452 None:intergenic 45.0%
TTCGAGGAACAGGATGATGA+AGG + chr7.4:27899801-27899820 None:intergenic 45.0%
! AGATGCGCTTTTCTTCGCTA+AGG - chr7.4:27899849-27899868 MS.gene058531:CDS 45.0%
! CCGATAGTGCCATTATCGAA+AGG - chr7.4:27900985-27901004 MS.gene058531:intron 45.0%
! CGAAGAAAAGCGCATCTCTA+AGG + chr7.4:27899846-27899865 None:intergenic 45.0%
! CGAGGTGAATTTTGCTTCCT+TGG + chr7.4:27899894-27899913 None:intergenic 45.0%
! GAGGTAGAAACATTGGCTGT+TGG - chr7.4:27901047-27901066 MS.gene058531:intron 45.0%
! GGACCGAAGTTTGTGGATTT+TGG - chr7.4:27901669-27901688 MS.gene058531:CDS 45.0%
! TCACAGTGTTGAGGATCTTG+AGG - chr7.4:27901028-27901047 MS.gene058531:intron 45.0%
! TGGTGGTATTCCTCCGTTTT+CGG + chr7.4:27902127-27902146 None:intergenic 45.0%
! TTTTCCGGGTTGCTTGATTC+TGG + chr7.4:27899610-27899629 None:intergenic 45.0%
!! ATTTTAGTCGCCGAAAACGG+AGG - chr7.4:27902114-27902133 MS.gene058531:CDS 45.0%
!! CTTCGGACAGGTTTTGTGAT+TGG + chr7.4:27901845-27901864 None:intergenic 45.0%
!! GCTTGGAAGAAGCACTTCTT+TGG - chr7.4:27901211-27901230 MS.gene058531:intron 45.0%
!! TTCTTGGTTTTCGGTTGGAG+TGG + chr7.4:27901989-27902008 None:intergenic 45.0%
AACCAAACAACGCCGTTTCG+AGG + chr7.4:27899817-27899836 None:intergenic 50.0%
AATCCACAGAGTCCGACTGT+TGG + chr7.4:27901535-27901554 None:intergenic 50.0%
AATGCACGTGCAACTCAGGA+GGG - chr7.4:27900881-27900900 MS.gene058531:CDS 50.0%
AGAATGCAATGGTGTGGTGG+TGG - chr7.4:27899645-27899664 MS.gene058531:CDS 50.0%
AGCTAATGCACGTGCAACTC+AGG - chr7.4:27900877-27900896 MS.gene058531:CDS 50.0%
CAACTGCAGCCGTAGATGAT+TGG + chr7.4:27901565-27901584 None:intergenic 50.0%
CAGGATGATGAAGGTCACGA+AGG + chr7.4:27899792-27899811 None:intergenic 50.0%
CATCTACGGCTGCAGTTGTA+GGG - chr7.4:27901567-27901586 MS.gene058531:intron 50.0%
CCAAGGACTCATTGAATCGG+TGG - chr7.4:27899678-27899697 MS.gene058531:CDS 50.0%
CTGTAGAGGACCGAAGTTTG+TGG - chr7.4:27901662-27901681 MS.gene058531:CDS 50.0%
GAAACGGCGTTGTTTGGTTG+AGG - chr7.4:27899818-27899837 MS.gene058531:CDS 50.0%
GTTGGAGCTTCACAGTGTTG+AGG - chr7.4:27901019-27901038 MS.gene058531:intron 50.0%
TAATGCACGTGCAACTCAGG+AGG - chr7.4:27900880-27900899 MS.gene058531:CDS 50.0%
TATCCAACAGTCGGACTCTG+TGG - chr7.4:27901529-27901548 MS.gene058531:intron 50.0%
TCATCTACGGCTGCAGTTGT+AGG - chr7.4:27901566-27901585 MS.gene058531:intron 50.0%
TCCAAAGGCTGCCTTGTCTT+CGG + chr7.4:27901862-27901881 None:intergenic 50.0%
TTAGGCATGGGAAGGTGAGA+TGG - chr7.4:27900089-27900108 MS.gene058531:intron 50.0%
TTCCTCGAAACGGCGTTGTT+TGG - chr7.4:27899812-27899831 MS.gene058531:CDS 50.0%
! TGTGTTTGAAACGACGAGCG+AGG + chr7.4:27899750-27899769 None:intergenic 50.0%
!! CCACCGATTCAATGAGTCCT+TGG + chr7.4:27899681-27899700 None:intergenic 50.0%
!! CTTGCGTTTTAGGCATGGGA+AGG - chr7.4:27900081-27900100 MS.gene058531:intron 50.0%
ACAACGCCGTTTCGAGGAAC+AGG + chr7.4:27899811-27899830 None:intergenic 55.0%
GGAAGGAACAGAAGAGAGGG+AGG - chr7.4:27899573-27899592 MS.gene058531:CDS 55.0%
TCCGAAGACAAGGCAGCCTT+TGG - chr7.4:27901858-27901877 MS.gene058531:intron 55.0%
TTGCGGCGAATACACCTTGC+CGG + chr7.4:27900327-27900346 None:intergenic 55.0%
!! ATGCAATGGTGTGGTGGTGG+TGG - chr7.4:27899648-27899667 MS.gene058531:CDS 55.0%
ACGTGCAACTCAGGAGGGCA+AGG - chr7.4:27900886-27900905 MS.gene058531:CDS 60.0%
AGGCTGCCTTGTCTTCGGAC+AGG + chr7.4:27901857-27901876 None:intergenic 60.0%
!! CAATGGTGTGGTGGTGGTGG+AGG - chr7.4:27899651-27899670 MS.gene058531:CDS 60.0%
!! GTGGTGGTGGAGGAGATCCA+AGG - chr7.4:27899661-27899680 MS.gene058531:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 27899571 27902282 27899571 ID=MS.gene058531
chr7.4 mRNA 27899571 27902282 27899571 ID=MS.gene058531.t1;Parent=MS.gene058531
chr7.4 exon 27901941 27902282 27901941 ID=MS.gene058531.t1.exon1;Parent=MS.gene058531.t1
chr7.4 CDS 27901941 27902282 27901941 ID=cds.MS.gene058531.t1;Parent=MS.gene058531.t1
chr7.4 exon 27901644 27901760 27901644 ID=MS.gene058531.t1.exon2;Parent=MS.gene058531.t1
chr7.4 CDS 27901644 27901760 27901644 ID=cds.MS.gene058531.t1;Parent=MS.gene058531.t1
chr7.4 exon 27900546 27900980 27900546 ID=MS.gene058531.t1.exon3;Parent=MS.gene058531.t1
chr7.4 CDS 27900546 27900980 27900546 ID=cds.MS.gene058531.t1;Parent=MS.gene058531.t1
chr7.4 exon 27899571 27900027 27899571 ID=MS.gene058531.t1.exon4;Parent=MS.gene058531.t1
chr7.4 CDS 27899571 27900027 27899571 ID=cds.MS.gene058531.t1;Parent=MS.gene058531.t1
Gene Sequence

>MS.gene058531

ATGGAAGGAACAGAAGAGAGGGAGGAAAATGTAGAACCAGAATCAAGCAACCCGGAAAATGAGAATGAAGAAGAAGAATGCAATGGTGTGGTGGTGGTGGAGGAGATCCAAGGACTCATTGAATCGGTGGTTCAATTCGGCGAATACAGAAGAACACAAAGGAAAGAATCACACAACCTCGCTCGTCGTTTCAAACACATGTTGCCTCTTATGGAAGACCTTCGTGACCTTCATCATCCTGTTCCTCGAAACGGCGTTGTTTGGTTGAGGAACCTTAGAGATGCGCTTTTCTTCGCTAAGGATTTGTTGAAATTGTGTAGCCAAGGAAGCAAAATTCACCTCGTAAATAACAAACAAACTTTTTCTCATTTTTATGTTTTTTTTCTTTTTCTAATGAAATAATACAAATCATCAAATCTATGATTTCTCTTTTTTAAGGATAAGAAATAATTATCATTTTTGTATTTGATTATGTTTTGATGAATAGGGTATTGATGTTTTCTTTTTTTTCTTGCGTTTTAGGCATGGGAAGGTGAGATGGTTATGATCAAATTTCAGAAGGTTTATGAAAAATTAAGCCAAGCTTTTGATGGTGTGCCTTGTGATGAATTAGGGATTTCTGATGAAGTAAAAGAACAGGTATTTGTAAATTGTAATACATTTTATGTTTTATTTTCATTTAGTTTAATAATCACTATGATTCTGCAAAGATTGTTCAATATATAATTATGTGTATGGAACCCTTATTTTATTCCGGCAAGGTGTATTCGCCGCAATACACCGCATTCACAAATACATGCATTCACATGGTCAATCGTTGGATCCAGATGTAGAAAACAAGCAGATTTTGATCCAACAACCACCAATGTGCAAATTGCACGAATGCACGGAATTTGTGACTATATCAAATGCAAATCCGCTCTTAATAATTACACTTTTGGTCACTGTTATAAGCAAAAAATCAGTTTTAGATTAATTGAATAACTGAAGTATTTGGTCTATAATATATACCAAATACACCATTTATTCAATAAACCTACAAAAATGATTTGTTGCTTTAATAATTACACATTTGGTCACTGTTATAAGCAAAAAATCATTTTTAGATTAATTGAATAACTGAAGTATTTTGGTCTATAATATAGACCAAATACACCAGTTATTCAATAAACCTACAAAAATGATTTGTTGCTTATAATAGTGATCGGATGGAGCAATTATCCGATCACTATGTTGCTAGTAGTCTTCGATCATATATAATATACATATTGAAATAATGTTATATACTTTGGCTTAATTCAGCTTGAGCTAATGCACGTGCAACTCAGGAGGGCAAGGAGAAGAACTGATACACAAGACATAGAGCTAGCAATGGATATGATGGTGGTGTTCTCTGATGAAGATGATCGTAATGCCGATAGTGCCATTATCGAAAGGCTTGCAAAAAAGTTGGAGCTTCACAGTGTTGAGGATCTTGAGGTAGAAACATTGGCTGTTGGAAACCTTGTTAGAGAAAGAAAGGGAAAACAAAGTGAGAGTACAAAAAAAATAATTGACCTTCTTAACAGATTCAAAAGGACTGCAGGCATGGAAGAAACCGATGTTATCTTTGATGATGATCATGCCATGCCTAATAAGATGCTTGGAAGAAGCACTTCTTTGGTCATTCCTCATGAGTTTCTGTGTCCAATAACACTTGAAATTATGTCTGATCCTGTTATTATTGCAAGTGGCCAGGTAATCACGAATTAAGTTCTGTAAAAGAATTTTAATTTTTTCACATTTAAGCCTAAGTTGGAAAGATACTAGTCATATTTATGAAAATGGATTCGATGCGGTCAATAATTTCTACTATAATCAATAGATCTCGACTGTTGCATACATATTGGACGGTTCATATTATGAATTCGTATTATAAATTTGATTCGGTGAAGTTCAAATATGAATCGTCCAATCTTTATCCAACAGTCGGACTCTGTGGATTCTGAAATCCAATCATCTACGGCTGCAGTTGTAGGGACTATTAGATTTAGAATGAGAATTTCTCACTCTTAATCTAACGGTCGCGACATATGCAACATCATAGTGCAGCTGTAGAGGACCGAAGTTTGTGGATTTTGGCCTTTAGAGAATCTGGAATGAAGAAAACTTAGGTTTTATCTCTATATAATAATGCTAACAAAGGTTTTGTTTCTCTTTTTCTTGTCATTGTGTGTGTTTGTTTCCATCAAAGACATATGAAAGGGAAAGCATAGAGAAGTGGTTCAAATCCCAATCACAAAACCTGTCCGAAGACAAGGCAGCCTTTGGAACATCTTCAATTAGCACCAAACTGTGCCTTAAGAAATTTAATTGCTGAATGGTGTGAGAACAACAATTTCAAACTTCCAAAAATCTGTTCTTCATGTCAAGAAACCACTCCAACCGAAAACCAAGAAGAAATTCCATGTTTGGTTGAAAACCTATCGTCGATCAACTTAGAACATCAAAGAAAGGCGGTCGAGAGAATCCGCATGTTATCAAAAGAAAGTTCAGAGAACAGAATTTTAGTCGCCGAAAACGGAGGAATACCACCATTAGTACAAATCCTATCATATCCAGATTCAAAAATAAAAGAATCATGCTGTCTTACAGCACTTTTGAACTTATCAATTGATGAAGCTAACAAAAAACTTATATCAAAAGAAGGTGCTATTCCAGCTATACATAGAA
Protein sequence

>MS.gene058531.t1

MEGTEEREENVEPESSNPENENEEEECNGVVVVEEIQGLIESVVQFGEYRRTQRKESHNLARRFKHMLPLMEDLRDLHHPVPRNGVVWLRNLRDALFFAKDLLKLCSQGSKIHLAWEGEMVMIKFQKVYEKLSQAFDGVPCDELGISDEVKEQLELMHVQLRRARRRTDTQDIELAMDMMVVFSDEDDRNADSAIIERLAKKLELHSVEDLEVETLAVGNLVRERKGKQSESTKKIIDLLNRFKRTAGMEETDVIFDDDHAMPNKMLGRSTSLVIPHEFLCPITLEIMSDPVIIASGQRSGSNPNHKTCPKTRQPLEHLQLAPNCALRNLIAEWCENNNFKLPKICSSCQETTPTENQEEIPCLVENLSSINLEHQRKAVERIRMLSKESSENRILVAENGGIPPLVQILSYPDSKIKESCCLTALLNLSIDEANKKLISKEGAIPAIHR