AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058533

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058533.t1 MTR_7g078330 79.236 602 41 8 33 567 26 610 0.0 896
MS.gene058533.t1 MTR_4g063800 34.983 606 288 13 45 566 12 595 3.37e-98 312
MS.gene058533.t1 MTR_1g094215 33.278 607 304 11 25 559 3 580 2.40e-77 256
MS.gene058533.t1 MTR_7g059405 31.491 597 302 14 49 560 21 595 8.95e-65 223
MS.gene058533.t1 MTR_4g485520 29.983 587 299 15 60 556 41 605 1.37e-54 196
MS.gene058533.t1 MTR_4g485520 33.144 353 156 6 267 556 102 437 6.04e-47 171
MS.gene058533.t1 MTR_1g094215 34.586 399 179 8 222 559 20 397 6.59e-46 167
MS.gene058533.t1 MTR_5g083030 25.997 627 328 15 49 556 31 640 2.01e-45 171
MS.gene058533.t1 MTR_2g018010 27.546 599 320 19 59 561 55 635 3.52e-42 161
MS.gene058533.t1 MTR_4g028960 28.780 410 228 12 92 459 93 480 6.55e-31 128
MS.gene058533.t1 MTR_3g078160 24.335 489 295 12 58 492 46 513 4.21e-27 115
MS.gene058533.t1 MTR_3g078160 24.540 489 294 12 58 492 46 513 5.24e-27 116
MS.gene058533.t1 MTR_3g078160 24.540 489 294 12 58 492 46 513 5.55e-27 116
MS.gene058533.t1 MTR_4g107010 23.108 489 304 11 58 493 52 521 7.91e-26 112
MS.gene058533.t1 MTR_1g093965 33.480 227 109 6 310 536 11 195 1.17e-25 105
MS.gene058533.t1 MTR_1g093995 33.040 227 110 6 310 536 11 195 2.68e-24 101
MS.gene058533.t1 MTR_7g053260 26.271 354 176 10 267 556 75 407 5.91e-24 105
MS.gene058533.t1 MTR_3g085610 27.143 350 166 11 73 363 37 356 2.64e-23 105
MS.gene058533.t1 MTR_7g116600 29.084 251 130 6 271 491 79 311 3.57e-23 103
MS.gene058533.t1 MTR_1g079450 28.358 268 143 7 267 505 60 307 9.77e-21 95.5
MS.gene058533.t1 MTR_8g077205 25.974 385 187 14 62 390 27 369 7.87e-20 94.0
MS.gene058533.t1 MTR_3g466220 36.190 210 89 6 379 543 552 761 1.86e-19 92.8
MS.gene058533.t1 MTR_3g466220 31.034 174 94 4 193 361 155 307 8.58e-16 81.3
MS.gene058533.t1 MTR_3g466220 36.190 210 89 6 379 543 531 740 2.12e-19 92.8
MS.gene058533.t1 MTR_3g466220 31.034 174 94 4 193 361 134 286 9.87e-16 81.3
MS.gene058533.t1 MTR_5g034440 22.459 610 320 20 52 561 48 604 3.77e-19 91.7
MS.gene058533.t1 MTR_3g103050 28.631 241 110 7 383 562 54 293 3.15e-16 80.5
MS.gene058533.t1 MTR_8g080280 36.792 106 50 1 258 363 270 358 3.60e-16 82.4
MS.gene058533.t1 MTR_1g018180 29.583 240 112 8 374 556 54 293 4.23e-16 80.5
MS.gene058533.t1 MTR_2g026130 32.663 199 89 5 404 557 210 408 2.11e-15 79.3
MS.gene058533.t1 MTR_1g069845 25.249 301 191 6 60 354 36 308 2.83e-15 79.7
MS.gene058533.t1 MTR_1g069845 33.498 203 91 6 404 562 475 677 5.58e-15 78.6
MS.gene058533.t1 MTR_8g077205 33.333 120 54 2 271 390 164 257 3.15e-15 79.3
MS.gene058533.t1 MTR_1g069845 25.249 301 191 6 60 354 40 312 3.56e-15 79.3
MS.gene058533.t1 MTR_1g069845 33.498 203 91 6 404 562 479 681 5.75e-15 78.6
MS.gene058533.t1 MTR_1g069845 33.498 203 91 6 404 562 358 560 4.84e-15 78.6
MS.gene058533.t1 MTR_1g069845 32.520 123 61 2 237 354 86 191 6.49e-14 75.1
MS.gene058533.t1 MTR_2g011140 28.216 241 129 8 276 492 21 241 9.02e-15 76.6
MS.gene058533.t1 MTR_7g117890 25.195 385 230 12 96 459 76 423 3.16e-14 76.6
MS.gene058533.t1 MTR_5g048050 38.889 90 38 1 268 357 23 95 5.26e-14 74.7
MS.gene058533.t1 MTR_3g065080 31.818 132 61 4 236 364 9 114 7.35e-14 74.3
MS.gene058533.t1 MTR_6g071340 37.778 90 39 1 268 357 3 75 1.15e-13 73.6
MS.gene058533.t1 MTR_7g097020 34.862 109 54 1 254 362 225 316 1.59e-13 73.9
MS.gene058533.t1 MTR_7g097020 30.916 262 118 9 364 562 458 719 2.77e-13 73.2
MS.gene058533.t1 MTR_1g056870 29.434 265 109 11 274 530 11 205 2.19e-13 72.8
MS.gene058533.t1 MTR_5g077510 37.931 87 37 1 268 354 33 102 2.40e-13 72.8
MS.gene058533.t1 MTR_1g056840 39.167 120 43 4 274 387 11 106 2.48e-13 72.4
MS.gene058533.t1 MTR_5g015210 26.238 202 112 4 270 459 67 243 3.67e-13 72.0
MS.gene058533.t1 MTR_1g056880 29.545 264 110 10 274 530 11 205 4.15e-13 72.0
MS.gene058533.t1 MTR_3g096370 39.773 88 36 1 266 353 6 76 5.75e-13 71.2
MS.gene058533.t1 MTR_4g085720 38.636 88 37 1 270 357 14 84 1.37e-12 70.1
MS.gene058533.t1 MTR_5g020570 29.457 129 65 2 262 390 265 367 1.44e-12 70.9
MS.gene058533.t1 MTR_8g027140 23.445 418 260 13 105 491 82 470 1.47e-12 71.2
MS.gene058533.t1 MTR_8g103227 30.994 171 92 5 217 386 350 495 1.74e-12 70.9
MS.gene058533.t1 MTR_5g020570 29.457 129 65 2 262 390 280 382 1.90e-12 70.5
MS.gene058533.t1 MTR_1g017770 24.138 203 116 5 270 459 45 222 1.26e-11 67.4
MS.gene058533.t1 MTR_1g056910 40.000 90 35 3 270 357 6 78 1.85e-11 66.6
MS.gene058533.t1 MTR_1g100820 37.143 105 44 3 249 353 630 712 4.70e-11 66.2
MS.gene058533.t1 MTR_1g100820 37.143 105 44 3 249 353 632 714 4.71e-11 66.2
MS.gene058533.t1 MTR_4g091880 40.000 85 33 2 270 353 5 72 6.40e-11 64.7
MS.gene058533.t1 MTR_4g091880 40.000 85 33 2 270 353 5 72 6.68e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058533.t1 AT5G42340 48.333 600 212 12 38 567 53 624 1.15e-167 491
MS.gene058533.t1 AT3G46510 36.256 593 282 11 45 559 15 589 2.18e-99 315
MS.gene058533.t1 AT3G54850 31.122 588 306 11 46 559 20 582 1.04e-76 255
MS.gene058533.t1 AT2G28830 32.605 549 260 16 108 566 1 529 2.35e-65 224
MS.gene058533.t1 AT1G23030 29.866 596 323 14 33 560 2 570 1.93e-59 208
MS.gene058533.t1 AT1G71020 29.133 611 319 18 33 560 2 581 4.54e-50 183
MS.gene058533.t1 AT1G71020 32.778 360 156 10 264 556 89 429 1.03e-37 145
MS.gene058533.t1 AT1G29340 26.766 538 300 17 32 492 26 546 9.54e-36 142
MS.gene058533.t1 AT5G01830 28.989 376 226 10 106 459 104 460 1.18e-32 133
MS.gene058533.t1 AT3G07360 29.972 357 166 13 271 561 75 413 9.49e-30 122
MS.gene058533.t1 AT1G67530 27.219 338 172 9 73 362 36 347 2.02e-25 110
MS.gene058533.t1 AT1G67530 27.859 341 166 10 73 362 36 347 2.64e-25 111
MS.gene058533.t1 AT1G67530 27.859 341 166 10 73 362 36 347 2.64e-25 111
MS.gene058533.t1 AT1G67530 27.859 341 166 10 73 362 36 347 2.64e-25 111
MS.gene058533.t1 AT1G27910 23.477 558 266 18 69 492 32 562 7.81e-25 109
MS.gene058533.t1 AT1G24330 27.778 342 168 12 73 362 36 350 8.97e-23 103
MS.gene058533.t1 AT1G24330 27.778 342 168 12 73 362 36 350 8.97e-23 103
MS.gene058533.t1 AT1G60190 25.103 486 289 16 58 490 50 513 2.03e-21 99.0
MS.gene058533.t1 AT2G23140 34.728 239 101 6 379 562 353 591 3.25e-21 98.2
MS.gene058533.t1 AT2G23140 38.384 99 44 1 264 362 35 116 1.64e-16 83.6
MS.gene058533.t1 AT2G23140 34.728 239 101 6 379 562 353 591 3.25e-21 98.2
MS.gene058533.t1 AT2G23140 38.384 99 44 1 264 362 35 116 1.64e-16 83.6
MS.gene058533.t1 AT2G23140 34.728 239 101 6 379 562 542 780 4.73e-21 98.2
MS.gene058533.t1 AT2G23140 36.607 112 52 2 264 373 224 318 1.47e-16 84.0
MS.gene058533.t1 AT2G23140 34.728 239 101 6 379 562 542 780 4.73e-21 98.2
MS.gene058533.t1 AT2G23140 36.607 112 52 2 264 373 224 318 1.47e-16 84.0
MS.gene058533.t1 AT2G23140 34.728 239 101 6 379 562 545 783 5.65e-21 97.8
MS.gene058533.t1 AT2G23140 36.607 112 52 2 264 373 227 321 1.49e-16 84.0
MS.gene058533.t1 AT1G10560 23.596 445 264 12 106 494 103 527 1.64e-19 92.8
MS.gene058533.t1 AT5G18320 27.385 325 167 9 270 543 72 378 2.55e-18 88.2
MS.gene058533.t1 AT3G19380 28.077 260 129 9 257 492 1 226 2.14e-16 82.0
MS.gene058533.t1 AT4G21350 27.500 320 165 10 270 543 5 303 1.05e-15 79.7
MS.gene058533.t1 AT3G01400 29.752 242 110 9 374 556 57 297 1.56e-15 79.0
MS.gene058533.t1 AT5G67340 28.571 266 132 8 355 562 396 661 7.32e-15 78.2
MS.gene058533.t1 AT5G67340 32.000 125 62 2 270 394 240 341 1.69e-13 73.9
MS.gene058533.t1 AT5G67340 28.571 266 132 8 355 562 396 661 7.32e-15 78.2
MS.gene058533.t1 AT5G67340 32.000 125 62 2 270 394 240 341 1.69e-13 73.9
MS.gene058533.t1 AT3G54790 35.176 199 84 7 409 562 514 712 1.24e-14 77.8
MS.gene058533.t1 AT3G54790 29.545 132 69 3 244 370 207 319 2.20e-12 70.5
MS.gene058533.t1 AT3G54790 35.176 199 84 7 409 562 514 712 1.24e-14 77.8
MS.gene058533.t1 AT3G54790 29.545 132 69 3 244 370 207 319 2.20e-12 70.5
MS.gene058533.t1 AT3G54790 35.176 199 84 7 409 562 478 676 1.28e-14 77.4
MS.gene058533.t1 AT3G54790 29.545 132 69 3 244 370 171 283 1.58e-12 70.9
MS.gene058533.t1 AT5G58680 28.319 226 104 6 388 556 71 295 1.40e-14 75.9
MS.gene058533.t1 AT1G49780 32.394 142 68 3 257 394 1 118 3.90e-14 75.1
MS.gene058533.t1 AT5G18330 25.591 254 137 6 270 493 65 296 2.59e-13 72.8
MS.gene058533.t1 AT1G66160 37.931 87 37 1 268 354 31 100 1.18e-12 70.5
MS.gene058533.t1 AT4G36550 23.052 308 185 7 66 361 26 293 1.29e-12 71.2
MS.gene058533.t1 AT5G65920 27.692 195 118 4 270 459 60 236 1.53e-12 70.1
MS.gene058533.t1 AT5G65920 27.692 195 118 4 270 459 60 236 1.53e-12 70.1
MS.gene058533.t1 AT4G16490 29.648 199 95 5 404 557 220 418 4.69e-12 68.9
MS.gene058533.t1 AT3G49810 27.273 198 115 5 270 459 64 240 6.78e-12 68.2
MS.gene058533.t1 AT2G45920 39.759 83 33 2 271 353 326 391 8.35e-12 67.8
MS.gene058533.t1 AT3G07360 27.957 279 134 12 349 561 1 278 8.35e-12 67.0
MS.gene058533.t1 AT3G07360 27.957 279 134 12 349 561 1 278 8.35e-12 67.0
MS.gene058533.t1 AT3G07360 27.957 279 134 12 349 561 1 278 8.35e-12 67.0
MS.gene058533.t1 AT3G07360 27.957 279 134 12 349 561 1 278 8.35e-12 67.0
MS.gene058533.t1 AT3G07360 27.957 279 134 12 349 561 1 278 8.35e-12 67.0
MS.gene058533.t1 AT3G07360 27.957 279 134 12 349 561 1 278 8.35e-12 67.0
MS.gene058533.t1 AT3G07360 27.957 279 134 12 349 561 1 278 8.35e-12 67.0
MS.gene058533.t1 AT3G07360 27.957 279 134 12 349 561 1 278 8.35e-12 67.0
MS.gene058533.t1 AT3G07360 27.957 279 134 12 349 561 1 278 8.35e-12 67.0
MS.gene058533.t1 AT1G20780 27.511 229 107 6 270 456 23 234 1.05e-11 68.2
MS.gene058533.t1 AT1G20780 27.511 229 107 6 270 456 23 234 1.05e-11 68.2
MS.gene058533.t1 AT1G20780 27.511 229 107 6 270 456 23 234 1.05e-11 68.2
MS.gene058533.t1 AT1G01680 40.449 89 35 3 273 360 236 307 2.41e-11 65.5

Find 109 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTCTTCATTCTCATTTC+CGG 0.131400 7.4:+27956243 None:intergenic
GACATAATTTCAAGTGTTAT+TGG 0.211508 7.4:+27954612 None:intergenic
CAAACACATGTTGCCTCTTA+TGG 0.212487 7.4:-27956104 MS.gene058533:CDS
ACCAAACATGGAATTTCTTC+TGG 0.213920 7.4:+27953867 None:intergenic
AGCTTTACTTCAATTGTTAA+AGG 0.235604 7.4:-27953361 MS.gene058533:CDS
TCTTCTTCATTCTCATTTCC+GGG 0.287231 7.4:+27956244 None:intergenic
TCCCTTTCATATGTCTTTGA+TGG 0.291405 7.4:+27954074 None:intergenic
CCCAGAAGAAATTCCATGTT+TGG 0.293161 7.4:-27953868 MS.gene058533:CDS
TGTAATAAATTTACCAAAGC+TGG 0.326531 7.4:+27953485 None:intergenic
TTGGTAAATTTATTACAAAA+TGG 0.326549 7.4:-27953479 MS.gene058533:CDS
ATTTCCGGGTTGCTTGATTC+TGG 0.328036 7.4:+27956258 None:intergenic
GCTTGGAAGAAGCACTTCTT+TGG 0.328604 7.4:-27954658 MS.gene058533:CDS
CTTCTTCCAAGCATCTTATT+AGG 0.336614 7.4:+27954669 None:intergenic
TTCCTCGAAACGGCGTTGTT+TGG 0.340973 7.4:-27956054 MS.gene058533:CDS
CCAACCTCATGCCTCCCTTC+AGG 0.349557 7.4:+27953280 None:intergenic
TTCAGCAATTAAATTTCTTA+AGG 0.357358 7.4:+27953962 None:intergenic
CGAAGAAAAGCGCATCTCTA+AGG 0.359752 7.4:+27956023 None:intergenic
GGTGTGCCTTGTGATGAATT+AGG 0.367708 7.4:-27955705 MS.gene058533:CDS
CCAAACATGGAATTTCTTCT+GGG 0.372000 7.4:+27953868 None:intergenic
GTCAAATGGGATTCCAGCTT+TGG 0.381074 7.4:-27953498 MS.gene058533:CDS
GTGTGCCTTGTGATGAATTA+GGG 0.381169 7.4:-27955704 MS.gene058533:CDS
TCCGAAGACAAGGCAGCCTT+TGG 0.394785 7.4:-27954012 MS.gene058533:CDS
ATTGAATCGGTGGTTCAATT+CGG 0.409503 7.4:-27956178 MS.gene058533:CDS
AACCAAACAACGCCGTTTCG+AGG 0.411594 7.4:+27956052 None:intergenic
AAATTTCTTAAGGCACAGTT+TGG 0.411871 7.4:+27953972 None:intergenic
TCCAAAGGCTGCCTTGTCTT+CGG 0.419783 7.4:+27954011 None:intergenic
ACAACGCCGTTTCGAGGAAC+AGG 0.427957 7.4:+27956058 None:intergenic
GAGGTTGTAGGAGTGTCAAA+TGG 0.430268 7.4:-27953512 MS.gene058533:CDS
GATGAAAATAAAGAGGTTGT+AGG 0.431054 7.4:-27953524 MS.gene058533:CDS
CAGAATTTAGTCGCCGAAAA+CGG 0.436780 7.4:-27953760 MS.gene058533:CDS
GCCATGCCTAATAAGATGCT+TGG 0.439094 7.4:-27954675 MS.gene058533:CDS
TCCAACACTTCTATTATAGC+TGG 0.443786 7.4:+27953607 None:intergenic
ATTGTTAAAGGACAGAAACT+TGG 0.447238 7.4:-27953349 MS.gene058533:CDS
CTTGAAATTATGTCTGATCC+TGG 0.451192 7.4:-27954603 MS.gene058533:CDS
TTCTGATGAAGTAAAAGAAC+AGG 0.452149 7.4:-27955679 MS.gene058533:intron
TAAGAAATTTAATTGCTGAA+TGG 0.452536 7.4:-27953959 MS.gene058533:CDS
CAATGGTGTGGTGGTGGTGG+AGG 0.458616 7.4:-27956215 MS.gene058533:CDS
ATGCAATGGTGTGGTGGTGG+TGG 0.460589 7.4:-27956218 MS.gene058533:CDS
ACATGCATGGTGCATGAAAC+AGG 0.469474 7.4:-27956326 None:intergenic
AACCTTGTTAGAGAAAGAAA+AGG 0.469867 7.4:-27954798 MS.gene058533:intron
TATAATAGAAGTGTTGGAAA+TGG 0.470123 7.4:-27953602 MS.gene058533:CDS
CCACCGATTCAATGAGTCCT+TGG 0.474954 7.4:+27956188 None:intergenic
TCCAGCTATAATAGAAGTGT+TGG 0.478569 7.4:-27953608 MS.gene058533:CDS
GGTTATTATTGCAAGTGGCC+AGG 0.479803 7.4:-27954582 MS.gene058533:intron
GAAACGGCGTTGTTTGGTTG+AGG 0.484598 7.4:-27956048 MS.gene058533:CDS
CCTTTCGATAATGGCACTAT+CGG 0.487902 7.4:+27954882 None:intergenic
AAAGAGAGAGCTATAAAAGC+TGG 0.489962 7.4:-27953393 MS.gene058533:intron
AGGCTGCCTTGTCTTCGGAC+AGG 0.490475 7.4:+27954016 None:intergenic
TATGATAGGATTTGTACTAA+TGG 0.494085 7.4:+27953727 None:intergenic
AGCTAATGCACGTGCAACTC+AGG 0.495077 7.4:-27954990 MS.gene058533:CDS
TCCAAGCATCTTATTAGGCA+TGG 0.497480 7.4:+27954674 None:intergenic
AGGTTGTAGGAGTGTCAAAT+GGG 0.498394 7.4:-27953511 MS.gene058533:CDS
CTACTTGTGTTAACTCCTGA+AGG 0.498929 7.4:-27953295 MS.gene058533:CDS
TCACGAAGGTCTTCCATAAG+AGG 0.499928 7.4:+27956091 None:intergenic
ATGAAACAGGTGACTTTCAA+TGG 0.506498 7.4:-27956313 MS.gene058533:CDS
ACCTTCTTAACAGATTCAAA+AGG 0.513046 7.4:-27954743 MS.gene058533:CDS
AATGATTGATGAAAATAAAG+AGG 0.519990 7.4:-27953531 MS.gene058533:CDS
AACAGATTCAAAAGGACTGC+AGG 0.520736 7.4:-27954735 MS.gene058533:CDS
AGAGCTAGCAATGGATATGA+TGG 0.525717 7.4:-27954935 MS.gene058533:CDS
ATTCAAAAGGACTGCAGGCA+TGG 0.529095 7.4:-27954730 MS.gene058533:CDS
TCCATCAAAGACATATGAAA+GGG 0.529147 7.4:-27954075 MS.gene058533:CDS
CATCATCCTGTTCCTCGAAA+CGG 0.531583 7.4:-27956064 MS.gene058533:CDS
TTCCATCAAAGACATATGAA+AGG 0.536197 7.4:-27954076 MS.gene058533:CDS
GATCCAAGGACTCATTGAAT+CGG 0.548729 7.4:-27956191 MS.gene058533:CDS
CAACTTAGAACATCAAAGAA+AGG 0.556469 7.4:-27953826 MS.gene058533:CDS
ATCCTGGTTATTATTGCAAG+TGG 0.558406 7.4:-27954587 MS.gene058533:CDS
GTTCTAAGTTGATCGACGAT+AGG 0.558574 7.4:+27953838 None:intergenic
TTAGGCATGGGAAGGTGAGA+TGG 0.558729 7.4:-27955778 MS.gene058533:intron
AGAATGCAATGGTGTGGTGG+TGG 0.564838 7.4:-27956221 MS.gene058533:CDS
ACCTTGTTAGAGAAAGAAAA+GGG 0.568955 7.4:-27954797 MS.gene058533:intron
AATGGAAGGAACAGAAGAGA+GGG 0.575343 7.4:-27956295 MS.gene058533:CDS
GGTTATGATCAAATTTCAGA+AGG 0.576249 7.4:-27955757 MS.gene058533:CDS
CCTGAAGGGAGGCATGAGGT+TGG 0.577701 7.4:-27953280 MS.gene058533:CDS
TTGTTGAAATTGTGTAGCCA+AGG 0.579205 7.4:-27955992 MS.gene058533:CDS
GATAGGATTTGTACTAATGG+TGG 0.579251 7.4:+27953730 None:intergenic
ACGTGCAACTCAGGAGGGCA+AGG 0.580025 7.4:-27954981 MS.gene058533:CDS
AAGGCACACCATCAAAAGCT+TGG 0.581211 7.4:+27955718 None:intergenic
AACAGGTGACTTTCAATGGA+AGG 0.586665 7.4:-27956309 MS.gene058533:CDS
ACAAAACCTGTCCGAAGACA+AGG 0.586908 7.4:-27954022 MS.gene058533:CDS
GGAAGGAACAGAAGAGAGGG+AGG 0.596520 7.4:-27956292 MS.gene058533:CDS
TTCGAGGAACAGGATGATGA+AGG 0.600018 7.4:+27956068 None:intergenic
GTTGGAGCTTCACAGTGTTG+AGG 0.602329 7.4:-27954848 MS.gene058533:CDS
TAATGCACGTGCAACTCAGG+AGG 0.604700 7.4:-27954987 MS.gene058533:CDS
TTCACAGTGTTGAGGATCTG+AGG 0.605110 7.4:-27954840 MS.gene058533:intron
CCAAGGACTCATTGAATCGG+TGG 0.611282 7.4:-27956188 MS.gene058533:CDS
TGTGTTTGAAACGACGAGCG+AGG 0.614739 7.4:+27956119 None:intergenic
TACTTGTGTTAACTCCTGAA+GGG 0.615594 7.4:-27953294 MS.gene058533:CDS
AAAGGGAAAGCATAGAGAAG+TGG 0.617041 7.4:-27954058 MS.gene058533:CDS
CCGATAGTGCCATTATCGAA+AGG 0.622407 7.4:-27954882 MS.gene058533:CDS
CAATGGAAGGAACAGAAGAG+AGG 0.623216 7.4:-27956296 MS.gene058533:CDS
AATTTAGTCGCCGAAAACGG+AGG 0.626580 7.4:-27953757 MS.gene058533:CDS
GGCCACTTGCAATAATAACC+AGG 0.636790 7.4:+27954585 None:intergenic
AGAAGAATGCAATGGTGTGG+TGG 0.641470 7.4:-27956224 MS.gene058533:CDS
ACAAGACATAGAGCTAGCAA+TGG 0.643295 7.4:-27954944 MS.gene058533:CDS
AATGCACGTGCAACTCAGGA+GGG 0.649570 7.4:-27954986 MS.gene058533:CDS
GTGGTGGTGGAGGAGATCCA+AGG 0.649832 7.4:-27956205 MS.gene058533:CDS
AGAACCAGAATCAAGCAACC+CGG 0.656438 7.4:-27956262 MS.gene058533:CDS
AACTCCTGAAGGGAGGCATG+AGG 0.660040 7.4:-27953284 MS.gene058533:CDS
ATCATCATCAAAGATAACAT+CGG 0.668304 7.4:+27954701 None:intergenic
AATGAAGAAGAAGAATGCAA+TGG 0.671502 7.4:-27956232 MS.gene058533:CDS
GCTAATTGAAGATGTTCCAA+AGG 0.672940 7.4:+27953996 None:intergenic
GAAATCCCTAATTCATCACA+AGG 0.673692 7.4:+27955699 None:intergenic
TTGTGTTAACTCCTGAAGGG+AGG 0.688054 7.4:-27953291 MS.gene058533:CDS
CAGGATGATGAAGGTCACGA+AGG 0.693076 7.4:+27956077 None:intergenic
ATTGGACACAGAAACTCATG+AGG 0.705096 7.4:+27954630 None:intergenic
CTTAGAACATCAAAGAAAGG+CGG 0.713277 7.4:-27953823 MS.gene058533:intron
GCTAGCAATGGATATGATGG+TGG 0.719121 7.4:-27954932 MS.gene058533:CDS
AGAAGAAGAATGCAATGGTG+TGG 0.735055 7.4:-27956227 MS.gene058533:CDS
GCGAATACAGAAGAACACAA+AGG 0.740376 7.4:-27956156 MS.gene058533:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAACTTATATCAAAAGA+AGG - chr7.4:27955828-27955847 MS.gene058533:intron 15.0%
!! TCAATATATAATTATGTGTA+TGG - chr7.4:27953884-27953903 MS.gene058533:CDS 15.0%
!! TGAAATAATGTTATATACTT+TGG - chr7.4:27954438-27954457 MS.gene058533:intron 15.0%
!! TTCGTATTATAAATTTGATT+CGG - chr7.4:27955072-27955091 MS.gene058533:intron 15.0%
!! TTGGTAAATTTATTACAAAA+TGG - chr7.4:27955986-27956005 MS.gene058533:CDS 15.0%
!! AATGATTGATGAAAATAAAG+AGG - chr7.4:27955934-27955953 MS.gene058533:intron 20.0%
!! ACTAGTCATATTTATGAAAA+TGG - chr7.4:27954970-27954989 MS.gene058533:CDS 20.0%
!! CTATATAATAATGCTAACAA+AGG - chr7.4:27955328-27955347 MS.gene058533:intron 20.0%
!! TAAGAAATTTAATTGCTGAA+TGG - chr7.4:27955506-27955525 MS.gene058533:intron 20.0%
!! TATTGAATAAATGGTGTATT+TGG + chr7.4:27954182-27954201 None:intergenic 20.0%
!! TGCTTTAATAATTACACATT+TGG - chr7.4:27954222-27954241 MS.gene058533:intron 20.0%
!! TTCAGCAATTAAATTTCTTA+AGG + chr7.4:27955506-27955525 None:intergenic 20.0%
!! TTGTAGGTTTATTGAATAAA+TGG + chr7.4:27954191-27954210 None:intergenic 20.0%
!!! AAAAGTGTAATTATTAAGAG+CGG + chr7.4:27954088-27954107 None:intergenic 20.0%
!!! AAGTAATTCATCTTTTACTT+TGG - chr7.4:27956303-27956322 MS.gene058533:CDS 20.0%
!!! AATTGAATAACTGAAGTATT+TGG - chr7.4:27954279-27954298 MS.gene058533:intron 20.0%
!!! TAATTGAATACTGAAGTATT+TGG - chr7.4:27954143-27954162 MS.gene058533:intron 20.0%
!!! TCTATGATTTCTCTTTTTTA+AGG - chr7.4:27953585-27953604 MS.gene058533:intron 20.0%
!!! TGATTATGTTTTGATGAATA+GGG - chr7.4:27953635-27953654 MS.gene058533:CDS 20.0%
!!! TGTTCTTTTATTTTTGAATC+TGG + chr7.4:27955765-27955784 None:intergenic 20.0%
!!! TTGATTATGTTTTGATGAAT+AGG - chr7.4:27953634-27953653 MS.gene058533:CDS 20.0%
! AGCTTTACTTCAATTGTTAA+AGG - chr7.4:27956104-27956123 MS.gene058533:CDS 25.0%
! ATCATCATCAAAGATAACAT+CGG + chr7.4:27954767-27954786 None:intergenic 25.0%
! ATTTCTCACTCTTAATCTAA+CGG - chr7.4:27955206-27955225 MS.gene058533:intron 25.0%
! TATGATAGGATTTGTACTAA+TGG + chr7.4:27955741-27955760 None:intergenic 25.0%
! TATTGAATAACTGGTGTATT+TGG + chr7.4:27954318-27954337 None:intergenic 25.0%
! TGTAATAAATTTACCAAAGC+TGG + chr7.4:27955983-27956002 None:intergenic 25.0%
! TTGTAGGTTTATTGAATAAC+TGG + chr7.4:27954327-27954346 None:intergenic 25.0%
!! AATAATGCTAACAAAGGTTT+TGG - chr7.4:27955334-27955353 MS.gene058533:intron 25.0%
!! GACATAATTTCAAGTGTTAT+TGG + chr7.4:27954856-27954875 None:intergenic 25.0%
!! GCTCTTAATAATTACACTTT+TGG - chr7.4:27954087-27954106 MS.gene058533:CDS 25.0%
!! TTGTTGCTTATAATAGTGAT+CGG - chr7.4:27954354-27954373 MS.gene058533:intron 25.0%
!! TTTTTGAATCTGGATATGAT+AGG + chr7.4:27955755-27955774 None:intergenic 25.0%
!!! AAAGTTGTTTGTTATTTACG+AGG + chr7.4:27953511-27953530 None:intergenic 25.0%
!!! AACTTTCTTTTGATAACATG+CGG + chr7.4:27955678-27955697 None:intergenic 25.0%
!!! AAGCAACAAATCATTTTTGT+AGG + chr7.4:27954207-27954226 None:intergenic 25.0%
!!! TATAATAGAAGTGTTGGAAA+TGG - chr7.4:27955863-27955882 MS.gene058533:intron 25.0%
!!! TCTTTTTTTTCTTGCGTTTT+AGG - chr7.4:27953669-27953688 MS.gene058533:CDS 25.0%
AAATTTCTTAAGGCACAGTT+TGG + chr7.4:27955496-27955515 None:intergenic 30.0%
AACCTTGTTAGAGAAAGAAA+AGG - chr7.4:27954667-27954686 MS.gene058533:CDS 30.0%
AATGAAGAAGAAGAATGCAA+TGG - chr7.4:27953233-27953252 MS.gene058533:intron 30.0%
AATGCATGTATTTGTGAATG+CGG + chr7.4:27953952-27953971 None:intergenic 30.0%
ACCTTCTTAACAGATTCAAA+AGG - chr7.4:27954722-27954741 MS.gene058533:CDS 30.0%
ACCTTGTTAGAGAAAGAAAA+GGG - chr7.4:27954668-27954687 MS.gene058533:CDS 30.0%
ATTGTTAAAGGACAGAAACT+TGG - chr7.4:27956116-27956135 MS.gene058533:CDS 30.0%
CAACTTAGAACATCAAAGAA+AGG - chr7.4:27955639-27955658 MS.gene058533:intron 30.0%
GATGAAAATAAAGAGGTTGT+AGG - chr7.4:27955941-27955960 MS.gene058533:intron 30.0%
GGTTATGATCAAATTTCAGA+AGG - chr7.4:27953708-27953727 MS.gene058533:CDS 30.0%
TACAAAATGGAACAGTTAGA+GGG - chr7.4:27955999-27956018 MS.gene058533:CDS 30.0%
TCCATCAAAGACATATGAAA+GGG - chr7.4:27955390-27955409 MS.gene058533:intron 30.0%
TTACAAAATGGAACAGTTAG+AGG - chr7.4:27955998-27956017 MS.gene058533:CDS 30.0%
TTCCATCAAAGACATATGAA+AGG - chr7.4:27955389-27955408 MS.gene058533:intron 30.0%
TTCTGATGAAGTAAAAGAAC+AGG - chr7.4:27953786-27953805 MS.gene058533:intron 30.0%
TTCTTCTTCATTCTCATTTC+CGG + chr7.4:27953225-27953244 None:intergenic 30.0%
TTTCACATTTAAGCCTAAGT+TGG - chr7.4:27954941-27954960 MS.gene058533:CDS 30.0%
! AAATTAAGCCAAGCTTTTGA+TGG - chr7.4:27953739-27953758 MS.gene058533:CDS 30.0%
! ATCTGGAATGAAGAAAACTT+AGG - chr7.4:27955297-27955316 MS.gene058533:intron 30.0%
! TCCTTTTGAATCTGTTAAGA+AGG + chr7.4:27954726-27954745 None:intergenic 30.0%
! TGACATGAAGAACAGATTTT+TGG + chr7.4:27955556-27955575 None:intergenic 30.0%
! TTATGAAAATGGATTCGATG+CGG - chr7.4:27954981-27955000 MS.gene058533:CDS 30.0%
!! TTCTGAAATCCAATCATCTA+CGG - chr7.4:27955148-27955167 MS.gene058533:intron 30.0%
!!! ATGGAACCCTTATTTTATTC+CGG - chr7.4:27953903-27953922 MS.gene058533:CDS 30.0%
AAAGAGAGAGCTATAAAAGC+TGG - chr7.4:27956072-27956091 MS.gene058533:CDS 35.0%
ACCAAACATGGAATTTCTTC+TGG + chr7.4:27955601-27955620 None:intergenic 35.0%
ACCTTGCCGGAATAAAATAA+GGG + chr7.4:27953912-27953931 None:intergenic 35.0%
ACTACTAGCAACATAGTGAT+CGG + chr7.4:27954393-27954412 None:intergenic 35.0%
ACTGTTGCATACATATTGGA+CGG - chr7.4:27955036-27955055 MS.gene058533:intron 35.0%
AGAACTTAATCGTGATTACC+TGG + chr7.4:27954904-27954923 None:intergenic 35.0%
ATCCTGGTTATTATTGCAAG+TGG - chr7.4:27954878-27954897 MS.gene058533:CDS 35.0%
ATGAAACAGGTGACTTTCAA+TGG - chr7.4:27953152-27953171 MS.gene058533:CDS 35.0%
ATTGAATCGGTGGTTCAATT+CGG - chr7.4:27953287-27953306 MS.gene058533:CDS 35.0%
CACAAATACATGCATTCACA+TGG - chr7.4:27953956-27953975 MS.gene058533:CDS 35.0%
CCAAACATGGAATTTCTTCT+GGG + chr7.4:27955600-27955619 None:intergenic 35.0%
CTTAGAACATCAAAGAAAGG+CGG - chr7.4:27955642-27955661 MS.gene058533:intron 35.0%
CTTCATTCCAGATTCTCTAA+AGG + chr7.4:27955290-27955309 None:intergenic 35.0%
CTTCTTCCAAGCATCTTATT+AGG + chr7.4:27954799-27954818 None:intergenic 35.0%
CTTGAAATTATGTCTGATCC+TGG - chr7.4:27954862-27954881 MS.gene058533:CDS 35.0%
CTTGTTGAGTTACTAGAGAA+GGG - chr7.4:27956230-27956249 MS.gene058533:CDS 35.0%
GAAATCCCTAATTCATCACA+AGG + chr7.4:27953769-27953788 None:intergenic 35.0%
GACTAGTATCTTTCCAACTT+AGG + chr7.4:27954957-27954976 None:intergenic 35.0%
GATAGGATTTGTACTAATGG+TGG + chr7.4:27955738-27955757 None:intergenic 35.0%
GCTAATTGAAGATGTTCCAA+AGG + chr7.4:27955472-27955491 None:intergenic 35.0%
TACTTGTGTTAACTCCTGAA+GGG - chr7.4:27956171-27956190 MS.gene058533:CDS 35.0%
TCCAACACTTCTATTATAGC+TGG + chr7.4:27955861-27955880 None:intergenic 35.0%
TCCAATCTTTATCCAACAGT+CGG - chr7.4:27955115-27955134 MS.gene058533:intron 35.0%
TCCCTTTCATATGTCTTTGA+TGG + chr7.4:27955394-27955413 None:intergenic 35.0%
TCTTCTTCATTCTCATTTCC+GGG + chr7.4:27953224-27953243 None:intergenic 35.0%
TCTTGTTGAGTTACTAGAGA+AGG - chr7.4:27956229-27956248 MS.gene058533:CDS 35.0%
TGCTTATAATAGTGATCGGA+TGG - chr7.4:27954358-27954377 MS.gene058533:intron 35.0%
TTGTTGAAATTGTGTAGCCA+AGG - chr7.4:27953473-27953492 MS.gene058533:intron 35.0%
! GATAGGTTTTCAACCAAACA+TGG + chr7.4:27955613-27955632 None:intergenic 35.0%
! TAGCTCTCTCTTTATTTGCA+TGG + chr7.4:27956065-27956084 None:intergenic 35.0%
! TCCAGCTATAATAGAAGTGT+TGG - chr7.4:27955857-27955876 MS.gene058533:intron 35.0%
! TCCCTTTTCTTTCTCTAACA+AGG + chr7.4:27954672-27954691 None:intergenic 35.0%
! TGGAATTTCTTCTGGGTTTT+CGG + chr7.4:27955593-27955612 None:intergenic 35.0%
! TTTTGCAAGCCTTTCGATAA+TGG + chr7.4:27954595-27954614 None:intergenic 35.0%
!! ATCTGCTTGTTTTCTACATC+TGG + chr7.4:27953995-27954014 None:intergenic 35.0%
!! ATTTTGGCCTTTAGAGAATC+TGG - chr7.4:27955280-27955299 MS.gene058533:intron 35.0%
!! TGTTGGAAATGGAAGTATTG+TGG - chr7.4:27955874-27955893 MS.gene058533:intron 35.0%
!! TTTTCTTGCGTTTTAGGCAT+GGG - chr7.4:27953675-27953694 MS.gene058533:CDS 35.0%
!! TTTTTCTTGCGTTTTAGGCA+TGG - chr7.4:27953674-27953693 MS.gene058533:CDS 35.0%
AACAGATTCAAAAGGACTGC+AGG - chr7.4:27954730-27954749 MS.gene058533:CDS 40.0%
AACAGGTGACTTTCAATGGA+AGG - chr7.4:27953156-27953175 MS.gene058533:CDS 40.0%
AATGGAAGGAACAGAAGAGA+GGG - chr7.4:27953170-27953189 MS.gene058533:CDS 40.0%
ACAAGACATAGAGCTAGCAA+TGG - chr7.4:27954521-27954540 MS.gene058533:intron 40.0%
AGAAGAAGAATGCAATGGTG+TGG - chr7.4:27953238-27953257 MS.gene058533:intron 40.0%
AGAAGGGACTCCAAAGAATA+AGG - chr7.4:27956246-27956265 MS.gene058533:CDS 40.0%
AGAGCTAGCAATGGATATGA+TGG - chr7.4:27954530-27954549 MS.gene058533:intron 40.0%
AGGCCAAAATCCACAAACTT+CGG + chr7.4:27955270-27955289 None:intergenic 40.0%
ATTGGACACAGAAACTCATG+AGG + chr7.4:27954838-27954857 None:intergenic 40.0%
CAAACACATGTTGCCTCTTA+TGG - chr7.4:27953361-27953380 MS.gene058533:CDS 40.0%
CACCTTGCCGGAATAAAATA+AGG + chr7.4:27953913-27953932 None:intergenic 40.0%
CAGAATTTAGTCGCCGAAAA+CGG - chr7.4:27955705-27955724 MS.gene058533:CDS 40.0%
CATTCGTGCAATTTGCACAT+TGG + chr7.4:27954034-27954053 None:intergenic 40.0%
CCCAGAAGAAATTCCATGTT+TGG - chr7.4:27955597-27955616 MS.gene058533:intron 40.0%
CGAAAGGCTTGCAAAAAAGT+TGG - chr7.4:27954599-27954618 MS.gene058533:CDS 40.0%
CTACTTGTGTTAACTCCTGA+AGG - chr7.4:27956170-27956189 MS.gene058533:CDS 40.0%
CTCGACTGTTGCATACATAT+TGG - chr7.4:27955032-27955051 MS.gene058533:intron 40.0%
GATCCAAGGACTCATTGAAT+CGG - chr7.4:27953274-27953293 MS.gene058533:CDS 40.0%
GCGAATACAGAAGAACACAA+AGG - chr7.4:27953309-27953328 MS.gene058533:CDS 40.0%
GCTGCACATTCCTTATTCTT+TGG + chr7.4:27956259-27956278 None:intergenic 40.0%
GTGTGCCTTGTGATGAATTA+GGG - chr7.4:27953761-27953780 MS.gene058533:CDS 40.0%
TCCAAGCATCTTATTAGGCA+TGG + chr7.4:27954794-27954813 None:intergenic 40.0%
TCCGACTGTTGGATAAAGAT+TGG + chr7.4:27955119-27955138 None:intergenic 40.0%
TTTGTGAATGCGGTGTATTG+CGG + chr7.4:27953942-27953961 None:intergenic 40.0%
! AAAGGGAAAGCATAGAGAAG+TGG - chr7.4:27955407-27955426 MS.gene058533:intron 40.0%
! ATTTCTTCTGGGTTTTCGGT+TGG + chr7.4:27955589-27955608 None:intergenic 40.0%
! ATTTGCACATTGGTGGTTGT+TGG + chr7.4:27954024-27954043 None:intergenic 40.0%
! CCTTTCGATAATGGCACTAT+CGG + chr7.4:27954586-27954605 None:intergenic 40.0%
!! AGGTTGTAGGAGTGTCAAAT+GGG - chr7.4:27955954-27955973 MS.gene058533:intron 40.0%
!! GTTCTAAGTTGATCGACGAT+AGG + chr7.4:27955630-27955649 None:intergenic 40.0%
!!! ACCCTTATTTTATTCCGGCA+AGG - chr7.4:27953908-27953927 MS.gene058533:CDS 40.0%
AAGGCACACCATCAAAAGCT+TGG + chr7.4:27953750-27953769 None:intergenic 45.0%
AATTTAGTCGCCGAAAACGG+AGG - chr7.4:27955708-27955727 MS.gene058533:CDS 45.0%
ACAAAACCTGTCCGAAGACA+AGG - chr7.4:27955443-27955462 MS.gene058533:intron 45.0%
ACATCATAGTGCAGCTGTAG+AGG - chr7.4:27955243-27955262 MS.gene058533:intron 45.0%
AGAACCAGAATCAAGCAACC+CGG - chr7.4:27953203-27953222 MS.gene058533:CDS 45.0%
AGAAGAATGCAATGGTGTGG+TGG - chr7.4:27953241-27953260 MS.gene058533:intron 45.0%
ATTCAAAAGGACTGCAGGCA+TGG - chr7.4:27954735-27954754 MS.gene058533:CDS 45.0%
ATTTCCGGGTTGCTTGATTC+TGG + chr7.4:27953210-27953229 None:intergenic 45.0%
CAATGGAAGGAACAGAAGAG+AGG - chr7.4:27953169-27953188 MS.gene058533:CDS 45.0%
CATCATCCTGTTCCTCGAAA+CGG - chr7.4:27953401-27953420 MS.gene058533:intron 45.0%
GCATTCACATGGTCAATCGT+TGG - chr7.4:27953967-27953986 MS.gene058533:CDS 45.0%
GCCATGCCTAATAAGATGCT+TGG - chr7.4:27954790-27954809 MS.gene058533:CDS 45.0%
GCTAGCAATGGATATGATGG+TGG - chr7.4:27954533-27954552 MS.gene058533:intron 45.0%
GGCCACTTGCAATAATAACC+AGG + chr7.4:27954883-27954902 None:intergenic 45.0%
GGTGTGCCTTGTGATGAATT+AGG - chr7.4:27953760-27953779 MS.gene058533:CDS 45.0%
GGTTATTATTGCAAGTGGCC+AGG - chr7.4:27954883-27954902 MS.gene058533:CDS 45.0%
GTCAAATGGGATTCCAGCTT+TGG - chr7.4:27955967-27955986 MS.gene058533:intron 45.0%
GTGCAAATTGCACGAATGCA+CGG - chr7.4:27954036-27954055 MS.gene058533:CDS 45.0%
TCACGAAGGTCTTCCATAAG+AGG + chr7.4:27953377-27953396 None:intergenic 45.0%
TCGTGCAATTTGCACATTGG+TGG + chr7.4:27954031-27954050 None:intergenic 45.0%
TTCGAGGAACAGGATGATGA+AGG + chr7.4:27953400-27953419 None:intergenic 45.0%
TTGTGTTAACTCCTGAAGGG+AGG - chr7.4:27956174-27956193 MS.gene058533:CDS 45.0%
! AGATGCGCTTTTCTTCGCTA+AGG - chr7.4:27953448-27953467 MS.gene058533:intron 45.0%
! CCGATAGTGCCATTATCGAA+AGG - chr7.4:27954583-27954602 MS.gene058533:CDS 45.0%
! CGAAGAAAAGCGCATCTCTA+AGG + chr7.4:27953445-27953464 None:intergenic 45.0%
! CGAGGTGAATTTTGCTTCCT+TGG + chr7.4:27953493-27953512 None:intergenic 45.0%
! GAGGATCTGAGGTAGAACAT+TGG - chr7.4:27954636-27954655 MS.gene058533:CDS 45.0%
! GGACCGAAGTTTGTGGATTT+TGG - chr7.4:27955264-27955283 MS.gene058533:intron 45.0%
! TGAGGTAGAACATTGGCTGT+TGG - chr7.4:27954643-27954662 MS.gene058533:CDS 45.0%
! TGGTGGTATTCCTCCGTTTT+CGG + chr7.4:27955721-27955740 None:intergenic 45.0%
! TTCACAGTGTTGAGGATCTG+AGG - chr7.4:27954625-27954644 MS.gene058533:CDS 45.0%
!! CTTCGGACAGGTTTTGTGAT+TGG + chr7.4:27955440-27955459 None:intergenic 45.0%
!! GAGGTTGTAGGAGTGTCAAA+TGG - chr7.4:27955953-27955972 MS.gene058533:intron 45.0%
!! GCTTGGAAGAAGCACTTCTT+TGG - chr7.4:27954807-27954826 MS.gene058533:intron 45.0%
AACCAAACAACGCCGTTTCG+AGG + chr7.4:27953416-27953435 None:intergenic 50.0%
AATCCACAGAGTCCGACTGT+TGG + chr7.4:27955130-27955149 None:intergenic 50.0%
AATGCACGTGCAACTCAGGA+GGG - chr7.4:27954479-27954498 MS.gene058533:intron 50.0%
AGAATGCAATGGTGTGGTGG+TGG - chr7.4:27953244-27953263 MS.gene058533:intron 50.0%
AGCTAATGCACGTGCAACTC+AGG - chr7.4:27954475-27954494 MS.gene058533:intron 50.0%
CAACTGCAGCCGTAGATGAT+TGG + chr7.4:27955160-27955179 None:intergenic 50.0%
CAGGATGATGAAGGTCACGA+AGG + chr7.4:27953391-27953410 None:intergenic 50.0%
CATCTACGGCTGCAGTTGTA+GGG - chr7.4:27955162-27955181 MS.gene058533:intron 50.0%
CCAAGGACTCATTGAATCGG+TGG - chr7.4:27953277-27953296 MS.gene058533:CDS 50.0%
CTGTAGAGGACCGAAGTTTG+TGG - chr7.4:27955257-27955276 MS.gene058533:intron 50.0%
GAAACGGCGTTGTTTGGTTG+AGG - chr7.4:27953417-27953436 MS.gene058533:intron 50.0%
GTTGGAGCTTCACAGTGTTG+AGG - chr7.4:27954617-27954636 MS.gene058533:CDS 50.0%
TAATGCACGTGCAACTCAGG+AGG - chr7.4:27954478-27954497 MS.gene058533:intron 50.0%
TATCCAACAGTCGGACTCTG+TGG - chr7.4:27955124-27955143 MS.gene058533:intron 50.0%
TCATCTACGGCTGCAGTTGT+AGG - chr7.4:27955161-27955180 MS.gene058533:intron 50.0%
TCCAAAGGCTGCCTTGTCTT+CGG + chr7.4:27955457-27955476 None:intergenic 50.0%
TTAGGCATGGGAAGGTGAGA+TGG - chr7.4:27953687-27953706 MS.gene058533:CDS 50.0%
TTCCTCGAAACGGCGTTGTT+TGG - chr7.4:27953411-27953430 MS.gene058533:intron 50.0%
! TGTGTTTGAAACGACGAGCG+AGG + chr7.4:27953349-27953368 None:intergenic 50.0%
! TTCTGGGTTTTCGGTTGGAG+TGG + chr7.4:27955584-27955603 None:intergenic 50.0%
!! CCACCGATTCAATGAGTCCT+TGG + chr7.4:27953280-27953299 None:intergenic 50.0%
!! CTTGCGTTTTAGGCATGGGA+AGG - chr7.4:27953679-27953698 MS.gene058533:CDS 50.0%
ACAACGCCGTTTCGAGGAAC+AGG + chr7.4:27953410-27953429 None:intergenic 55.0%
GGAAGGAACAGAAGAGAGGG+AGG - chr7.4:27953173-27953192 MS.gene058533:CDS 55.0%
TCCGAAGACAAGGCAGCCTT+TGG - chr7.4:27955453-27955472 MS.gene058533:intron 55.0%
TTGCGGCGAATACACCTTGC+CGG + chr7.4:27953925-27953944 None:intergenic 55.0%
! AACTCCTGAAGGGAGGCATG+AGG - chr7.4:27956181-27956200 MS.gene058533:CDS 55.0%
!! ATGCAATGGTGTGGTGGTGG+TGG - chr7.4:27953247-27953266 MS.gene058533:intron 55.0%
ACGTGCAACTCAGGAGGGCA+AGG - chr7.4:27954484-27954503 MS.gene058533:intron 60.0%
AGGCTGCCTTGTCTTCGGAC+AGG + chr7.4:27955452-27955471 None:intergenic 60.0%
CCAACCTCATGCCTCCCTTC+AGG + chr7.4:27956188-27956207 None:intergenic 60.0%
! CCTGAAGGGAGGCATGAGGT+TGG - chr7.4:27956185-27956204 MS.gene058533:CDS 60.0%
!! CAATGGTGTGGTGGTGGTGG+AGG - chr7.4:27953250-27953269 MS.gene058533:intron 60.0%
!! GTGGTGGTGGAGGAGATCCA+AGG - chr7.4:27953260-27953279 MS.gene058533:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 27953152 27956335 27953152 ID=MS.gene058533
chr7.4 mRNA 27953152 27956335 27953152 ID=MS.gene058533.t1;Parent=MS.gene058533
chr7.4 exon 27955976 27956335 27955976 ID=MS.gene058533.t1.exon1;Parent=MS.gene058533.t1
chr7.4 CDS 27955976 27956335 27955976 ID=cds.MS.gene058533.t1;Parent=MS.gene058533.t1
chr7.4 exon 27955680 27955796 27955680 ID=MS.gene058533.t1.exon2;Parent=MS.gene058533.t1
chr7.4 CDS 27955680 27955796 27955680 ID=cds.MS.gene058533.t1;Parent=MS.gene058533.t1
chr7.4 exon 27954841 27955016 27954841 ID=MS.gene058533.t1.exon3;Parent=MS.gene058533.t1
chr7.4 CDS 27954841 27955016 27954841 ID=cds.MS.gene058533.t1;Parent=MS.gene058533.t1
chr7.4 exon 27954583 27954809 27954583 ID=MS.gene058533.t1.exon4;Parent=MS.gene058533.t1
chr7.4 CDS 27954583 27954809 27954583 ID=cds.MS.gene058533.t1;Parent=MS.gene058533.t1
chr7.4 exon 27953824 27954137 27953824 ID=MS.gene058533.t1.exon5;Parent=MS.gene058533.t1
chr7.4 CDS 27953824 27954137 27953824 ID=cds.MS.gene058533.t1;Parent=MS.gene058533.t1
chr7.4 exon 27953595 27953791 27953595 ID=MS.gene058533.t1.exon6;Parent=MS.gene058533.t1
chr7.4 CDS 27953595 27953791 27953595 ID=cds.MS.gene058533.t1;Parent=MS.gene058533.t1
chr7.4 exon 27953475 27953559 27953475 ID=MS.gene058533.t1.exon7;Parent=MS.gene058533.t1
chr7.4 CDS 27953475 27953559 27953475 ID=cds.MS.gene058533.t1;Parent=MS.gene058533.t1
chr7.4 exon 27953255 27953407 27953255 ID=MS.gene058533.t1.exon8;Parent=MS.gene058533.t1
chr7.4 CDS 27953255 27953407 27953255 ID=cds.MS.gene058533.t1;Parent=MS.gene058533.t1
chr7.4 exon 27953152 27953225 27953152 ID=MS.gene058533.t1.exon9;Parent=MS.gene058533.t1
chr7.4 CDS 27953152 27953225 27953152 ID=cds.MS.gene058533.t1;Parent=MS.gene058533.t1
Gene Sequence

>MS.gene058533

ATGAAACAGGTGACTTTCAATGGAAGGAACAGAAGAGAGGGAGGAAAATGTAGAACCAGAATCAAGCAACCCGGAAATGAGAATGAAGAAGAAGAATGCAATGGTGTGGTGGTGGTGGAGGAGATCCAAGGACTCATTGAATCGGTGGTTCAATTCGGCGAATACAGAAGAACACAAAGGAAAGAATCACACAACCTCGCTCGTCGTTTCAAACACATGTTGCCTCTTATGGAAGACCTTCGTGACCTTCATCATCCTGTTCCTCGAAACGGCGTTGTTTGGTTGAGGAACCTTAGAGATGCGCTTTTCTTCGCTAAGGATTTGTTGAAATTGTGTAGCCAAGGAAGCAAAATTCACCTCGTAAATAACAAACAACTTTTTCTCATTTTTATGTTTTTTTTCTTTTTCTAATGAAATAATACAAATCATCAAATCTATGATTTCTCTTTTTTAAGGATAAGAAATAATTATCATTTTTGTATTTGATTATGTTTTGATGAATAGGGTATTGATGTTTTCTTTTTTTTCTTGCGTTTTAGGCATGGGAAGGTGAGATGGTTATGATCAAATTTCAGAAGGTTTATGAAAAATTAAGCCAAGCTTTTGATGGTGTGCCTTGTGATGAATTAGGGATTTCTGATGAAGTAAAAGAACAGGTATTTGTAAATTGTAATACATTTTATGTTTTATTTTCATTTAGTTTAATAATCACTATGATTCTGCAAAGATTGTTCAATATATAATTATGTGTATGGAACCCTTATTTTATTCCGGCAAGGTGTATTCGCCGCAATACACCGCATTCACAAATACATGCATTCACATGGTCAATCGTTGGATCCAGATGTAGAAAACAAGCAGATTTTGATCCAACAACCACCAATGTGCAAATTGCACGAATGCACGGAATTTGTGACTATATCAAATGCAAATCCGCTCTTAATAATTACACTTTTGGTCACTGTTATAAGCAAAAAAATCAGTTTTAGATTAATTGAATACTGAAGTATTTGGTCTATAATATATACCAAATACACCATTTATTCAATAAACCTACAAAAATGATTTGTTGCTTTAATAATTACACATTTGGTCACTGTTATAAGCAAAAAAATCATTTTTAGATTAATTGAATAACTGAAGTATTTGGTCTATAATATAGACCAAATACACCAGTTATTCAATAAACCTACAAAAATGATTTGTTGCTTATAATAGTGATCGGATGGAGCAATTATCCGATCACTATGTTGCTAGTAGTCTTCGATCATATATAATATACATATTGAAATAATGTTATATACTTTGGCTTAATTCAGCTTGAGCTAATGCACGTGCAACTCAGGAGGGCAAGGAGAAGAACTGATACACAAGACATAGAGCTAGCAATGGATATGATGGTGGTGTTCTCTGATGAAGATGATCGTAATGCCGATAGTGCCATTATCGAAAGGCTTGCAAAAAAGTTGGAGCTTCACAGTGTTGAGGATCTGAGGTAGAACATTGGCTGTTGGAAACCTTGTTAGAGAAAGAAAAGGGAAACAAAGTGAGAGTACAAAAAAAATAATTGACCTTCTTAACAGATTCAAAAGGACTGCAGGCATGGAAGAAACCGATGTTATCTTTGATGATGATCATGCCATGCCTAATAAGATGCTTGGAAGAAGCACTTCTTTGGTCATTCCTCATGAGTTTCTGTGTCCAATAACACTTGAAATTATGTCTGATCCTGGTTATTATTGCAAGTGGCCAGGTAATCACGATTAAGTTCTGTAAAAGAATTTTAATTTTTCACATTTAAGCCTAAGTTGGAAAGATACTAGTCATATTTATGAAAATGGATTCGATGCGGTCAATAATTTCTACTATAATCAATAGATCTCGACTGTTGCATACATATTGGACGGTTCATATTATGAATTCGTATTATAAATTTGATTCGGTGAAGTTCAAATATGAATCGTCCAATCTTTATCCAACAGTCGGACTCTGTGGATTCTGAAATCCAATCATCTACGGCTGCAGTTGTAGGGACTATTAGATTTAGAATGAGAATTTCTCACTCTTAATCTAACGGTCGCGACATATGCAACATCATAGTGCAGCTGTAGAGGACCGAAGTTTGTGGATTTTGGCCTTTAGAGAATCTGGAATGAAGAAAACTTAGGTTTTATCTCTATATAATAATGCTAACAAAGGTTTTGGTTTCTCTTTTTCTTGTCATTGTGTGTGTTTGTTTCCATCAAAGACATATGAAAGGGAAAGCATAGAGAAGTGGTTCAAATCCAATCACAAAACCTGTCCGAAGACAAGGCAGCCTTTGGAACATCTTCAATTAGCACCAAACTGTGCCTTAAGAAATTTAATTGCTGAATGGTGTGAGAACAACAATTTCAAACTTCCAAAAATCTGTTCTTCATGTCAAGAAACCACTCCAACCGAAAACCCAGAAGAAATTCCATGTTTGGTTGAAAACCTATCGTCGATCAACTTAGAACATCAAAGAAAGGCGGTCGAGAGAATCCGCATGTTATCAAAAGAAAGTTCAGAGAACAGAATTTAGTCGCCGAAAACGGAGGAATACCACCATTAGTACAAATCCTATCATATCCAGATTCAAAAATAAAAGAACATGCTGTTACAGCACTTTTGAACTTATCAATTGATGAAGCTAACAAAAAACTTATATCAAAAGAAGGTGCTATTCCAGCTATAATAGAAGTGTTGGAAATGGAAGTATTGTGGCTAAAGAAAACTCTGCAGCAGCTTTATTTAGCTTATCAATGATTGATGAAAATAAAGAGGTTGTAGGAGTGTCAAATGGGATTCCAGCTTTGGTAAATTTATTACAAAATGGAACAGTTAGAGGGAAAAAAGATGCTGCTACAGCACTTTTAGCTTATCATTAAACCATGCAAATAAAGAGAGAGCTATAAAAGCTGGAATTGTGACAGCTTTACTTCAATTGTTAAAGGACAGAAACTTGGAATGATTGATGAAGCACTTTCTATTTTGTTGCTACTTGTGTTAACTCCTGAAGGGAGGCATGAGGTTGGACAATTATCATTTATTGAAACTCTTGTTGAGTTACTAGAGAAGGGACTCCAAAGAATAAGGAATGTGCAGCTTCTGTTCTTCTTGAGTTGTGTTCAAGTAATTCATCTTTTACTTTGGCAGCACTTCA
Protein sequence

>MS.gene058533.t1

MKQVTFNGRNRREGGKCRTRIKQPGNENEEEECNGVVVVEEIQGLIESVVQFGEYRRTQRKESHNLARRFKHMLPLMEDLRDLHHPVPRNGVVWLRNLRDALFFAKDLLKLCSQGSKIHLAWEGEMVMIKFQKVYEKLSQAFDGVPCDELGISDEVKEQLELMHVQLRRARRRTDTQDIELAMDMMVVFSDEDDRNADSAIIERLAKKLELHSVEDLRERKGKQSESTKKIIDLLNRFKRTAGMEETDVIFDDDHAMPNKMLGRSTSLVIPHEFLCPITLEIMSDPGYYCKWPGFGFSFSCHCVCLFPSKTYERESIEKWFKSNHKTCPKTRQPLEHLQLAPNCALRNLIAEWCENNNFKLPKICSSCQETTPTENPEEIPCLVENLSSINLEHQRKAFREQNLVAENGGIPPLVQILSYPDSKIKEHAVTALLNLSIDEANKKLISKEGAIPAIIEVLEMEVFLSMIDENKEVVGVSNGIPALVNLLQNGTSYKSWNCDSFTSIVKGQKLGMIDEALSILLLLVLTPEGRHEVGQLSFIETLNKECAASVLLELCSSNSSFTLAAL