Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059081.t1 | XP_003588620.2 | 99.4 | 179 | 1 | 0 | 1 | 179 | 137 | 315 | 1.70E-92 | 348.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059081.t1 | G7I570 | 99.4 | 179 | 1 | 0 | 1 | 179 | 137 | 315 | 1.2e-92 | 348.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059080 | MS.gene059081 | 0.824733 | 6.88E-54 | -1.69E-46 |
| MS.gene059081 | MS.gene059082 | 0.844815 | 5.98E-59 | -1.69E-46 |
| MS.gene059081 | MS.gene059462 | 0.846876 | 1.65E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAGCAGGTGTTATATTTA+AGG | 0.203283 | 1.3:+10788049 | MS.gene059081:CDS |
| GAGAAGACATTCTTCAATTC+TGG | 0.242722 | 1.3:+10788200 | MS.gene059081:CDS |
| GGGTTCTCTATTGCTAGTTT+TGG | 0.258616 | 1.3:+10788166 | MS.gene059081:CDS |
| TGAGGATTAGAAGGCAATTA+TGG | 0.287173 | 1.3:+10788078 | MS.gene059081:CDS |
| TACTATGAGTGTTAGTCAAT+TGG | 0.328654 | 1.3:+10788145 | MS.gene059081:CDS |
| TTGTATTTATGTTACTTTCA+TGG | 0.331455 | 1.3:+10787916 | MS.gene059081:CDS |
| CTTCAAATATTGAGATTTGA+AGG | 0.390973 | 1.3:+10787996 | MS.gene059081:CDS |
| AGGAACATGTGAGGATTAGA+AGG | 0.398671 | 1.3:+10788069 | MS.gene059081:CDS |
| CTTTCATGGCTGTACAGAAC+TGG | 0.398954 | 1.3:+10787930 | MS.gene059081:CDS |
| ACTATGAGTGTTAGTCAATT+GGG | 0.421702 | 1.3:+10788146 | MS.gene059081:CDS |
| ACAAACACAATTGAGTGTAT+TGG | 0.427450 | 1.3:-10787897 | None:intergenic |
| GGAAATCCAGCACTAAGGTT+TGG | 0.455769 | 1.3:-10787876 | None:intergenic |
| CAAATCTCAATATTTGAAGC+TGG | 0.469941 | 1.3:-10787992 | None:intergenic |
| TCAGCACCAAACCTTAGTGC+TGG | 0.495418 | 1.3:+10787870 | MS.gene059081:CDS |
| AATTATGGATTATAAGTCAT+AGG | 0.501167 | 1.3:+10788093 | MS.gene059081:CDS |
| GAAGGGTGTGGATCAGAAGC+AGG | 0.525748 | 1.3:+10788035 | MS.gene059081:CDS |
| CTAAGAATAGAAGTTGCCTT+AGG | 0.539238 | 1.3:-10787701 | None:intergenic |
| CTTCAATTCTGGTGACCTTG+TGG | 0.547529 | 1.3:+10788211 | MS.gene059081:CDS |
| GCAAGTTCTACAAAGAAAGA+AGG | 0.565294 | 1.3:-10787813 | None:intergenic |
| GTTCTACAAAGAAAGAAGGT+AGG | 0.566351 | 1.3:-10787809 | None:intergenic |
| ATTCTTTATAATCTGTTGTG+TGG | 0.569662 | 1.3:+10788121 | MS.gene059081:CDS |
| GTATTGGAAATCCAGCACTA+AGG | 0.581099 | 1.3:-10787881 | None:intergenic |
| TATGATGCAGGTATGGCCTA+AGG | 0.582377 | 1.3:+10787685 | None:intergenic |
| GAATGCTTTCTCTAGCTCGC+GGG | 0.594449 | 1.3:-10787766 | None:intergenic |
| TGAATGCTTTCTCTAGCTCG+CGG | 0.629956 | 1.3:-10787767 | None:intergenic |
| TTATATTTAAGGAACATGTG+AGG | 0.659712 | 1.3:+10788060 | MS.gene059081:CDS |
| GACACTACATATGTTCGTCG+CGG | 0.737129 | 1.3:+10787738 | MS.gene059081:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTATGGATTATAAGTCAT+AGG | + | chr1.3:10788093-10788112 | MS.gene059081:CDS | 20.0% |
| !!! | TTGTATTTATGTTACTTTCA+TGG | + | chr1.3:10787916-10787935 | MS.gene059081:CDS | 20.0% |
| ! | ATTCTTTATAATCTGTTGTG+TGG | + | chr1.3:10788121-10788140 | MS.gene059081:CDS | 25.0% |
| ! | CTTCAAATATTGAGATTTGA+AGG | + | chr1.3:10787996-10788015 | MS.gene059081:CDS | 25.0% |
| ! | TTATATTTAAGGAACATGTG+AGG | + | chr1.3:10788060-10788079 | MS.gene059081:CDS | 25.0% |
| ACAAACACAATTGAGTGTAT+TGG | - | chr1.3:10787900-10787919 | None:intergenic | 30.0% | |
| ACTATGAGTGTTAGTCAATT+GGG | + | chr1.3:10788146-10788165 | MS.gene059081:CDS | 30.0% | |
| TACTATGAGTGTTAGTCAAT+TGG | + | chr1.3:10788145-10788164 | MS.gene059081:CDS | 30.0% | |
| ! | AGAAGCAGGTGTTATATTTA+AGG | + | chr1.3:10788049-10788068 | MS.gene059081:CDS | 30.0% |
| ! | CAAATCTCAATATTTGAAGC+TGG | - | chr1.3:10787995-10788014 | None:intergenic | 30.0% |
| CTAAGAATAGAAGTTGCCTT+AGG | - | chr1.3:10787704-10787723 | None:intergenic | 35.0% | |
| GAGAAGACATTCTTCAATTC+TGG | + | chr1.3:10788200-10788219 | MS.gene059081:CDS | 35.0% | |
| GCAAGTTCTACAAAGAAAGA+AGG | - | chr1.3:10787816-10787835 | None:intergenic | 35.0% | |
| GTTCTACAAAGAAAGAAGGT+AGG | - | chr1.3:10787812-10787831 | None:intergenic | 35.0% | |
| TGAGGATTAGAAGGCAATTA+TGG | + | chr1.3:10788078-10788097 | MS.gene059081:CDS | 35.0% | |
| ! | GTGTTCACAAGCTTTTTGAA+GGG | + | chr1.3:10788018-10788037 | MS.gene059081:CDS | 35.0% |
| AGGAACATGTGAGGATTAGA+AGG | + | chr1.3:10788069-10788088 | MS.gene059081:CDS | 40.0% | |
| GTATTGGAAATCCAGCACTA+AGG | - | chr1.3:10787884-10787903 | None:intergenic | 40.0% | |
| ! | GGTGTTCACAAGCTTTTTGA+AGG | + | chr1.3:10788017-10788036 | MS.gene059081:CDS | 40.0% |
| !! | GGGTTCTCTATTGCTAGTTT+TGG | + | chr1.3:10788166-10788185 | MS.gene059081:CDS | 40.0% |
| CTTCAATTCTGGTGACCTTG+TGG | + | chr1.3:10788211-10788230 | MS.gene059081:CDS | 45.0% | |
| CTTTCATGGCTGTACAGAAC+TGG | + | chr1.3:10787930-10787949 | MS.gene059081:CDS | 45.0% | |
| GACACTACATATGTTCGTCG+CGG | + | chr1.3:10787738-10787757 | MS.gene059081:CDS | 45.0% | |
| GGAAATCCAGCACTAAGGTT+TGG | - | chr1.3:10787879-10787898 | None:intergenic | 45.0% | |
| ! | TGAATGCTTTCTCTAGCTCG+CGG | - | chr1.3:10787770-10787789 | None:intergenic | 45.0% |
| !! | CACAAGCTTTTTGAAGGGTG+TGG | + | chr1.3:10788023-10788042 | MS.gene059081:CDS | 45.0% |
| TCAGCACCAAACCTTAGTGC+TGG | + | chr1.3:10787870-10787889 | MS.gene059081:CDS | 50.0% | |
| ! | GAATGCTTTCTCTAGCTCGC+GGG | - | chr1.3:10787769-10787788 | None:intergenic | 50.0% |
| ! | GAAGGGTGTGGATCAGAAGC+AGG | + | chr1.3:10788035-10788054 | MS.gene059081:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 10787695 | 10788232 | 10787695 | ID=MS.gene059081 |
| chr1.3 | mRNA | 10787695 | 10788232 | 10787695 | ID=MS.gene059081.t1;Parent=MS.gene059081 |
| chr1.3 | exon | 10787695 | 10788232 | 10787695 | ID=MS.gene059081.t1.exon1;Parent=MS.gene059081.t1 |
| chr1.3 | CDS | 10787695 | 10788232 | 10787695 | ID=cds.MS.gene059081.t1;Parent=MS.gene059081.t1 |
| Gene Sequence |
| Protein sequence |
