Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05909.t1 | XP_003630784.1 | 97.8 | 270 | 6 | 0 | 1 | 270 | 1 | 270 | 6.60E-144 | 520 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05909.t1 | F4IUE7 | 74.8 | 254 | 57 | 2 | 11 | 257 | 3 | 256 | 1.7e-100 | 367.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05909.t1 | G7L892 | 97.8 | 270 | 6 | 0 | 1 | 270 | 1 | 270 | 4.7e-144 | 520.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05909 | MS.gene069689 | 0.810988 | 8.76E-51 | -1.69E-46 |
| MS.gene05909 | MS.gene83831 | -0.807199 | 5.68E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05909.t1 | MTR_8g103400 | 98.519 | 270 | 4 | 0 | 1 | 270 | 1 | 270 | 0.0 | 541 |
| MS.gene05909.t1 | MTR_8g103420 | 90.189 | 265 | 24 | 1 | 8 | 270 | 1 | 265 | 9.64e-179 | 492 |
| MS.gene05909.t1 | MTR_8g103400 | 97.970 | 197 | 4 | 0 | 1 | 197 | 1 | 197 | 4.16e-140 | 392 |
| MS.gene05909.t1 | MTR_1g022150 | 70.565 | 248 | 66 | 1 | 19 | 259 | 3 | 250 | 1.24e-127 | 363 |
| MS.gene05909.t1 | MTR_3g095210 | 63.710 | 248 | 80 | 3 | 27 | 270 | 10 | 251 | 1.43e-117 | 337 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05909.t1 | AT2G20120 | 76.078 | 255 | 54 | 2 | 11 | 258 | 3 | 257 | 1.17e-137 | 389 |
| MS.gene05909.t1 | AT2G20120 | 76.078 | 255 | 54 | 2 | 11 | 258 | 3 | 257 | 1.17e-137 | 389 |
| MS.gene05909.t1 | AT2G20130 | 79.752 | 242 | 45 | 1 | 21 | 258 | 6 | 247 | 3.50e-137 | 387 |
| MS.gene05909.t1 | AT2G18460 | 72.428 | 243 | 63 | 1 | 21 | 259 | 6 | 248 | 9.29e-131 | 371 |
| MS.gene05909.t1 | AT1G43130 | 69.196 | 224 | 65 | 1 | 34 | 257 | 23 | 242 | 4.49e-113 | 326 |
| MS.gene05909.t1 | AT1G43130 | 74.372 | 199 | 51 | 0 | 59 | 257 | 11 | 209 | 3.12e-111 | 320 |
| MS.gene05909.t1 | AT1G43130 | 74.372 | 199 | 51 | 0 | 59 | 257 | 11 | 209 | 3.12e-111 | 320 |
Find 78 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGCAGAAATTGTCGTTTC+TGG | 0.156987 | 4.4:-3091713 | MS.gene05909:intron |
| CACCTCGTCTGTTGTCCTTC+AGG | 0.236531 | 4.4:-3094619 | MS.gene05909:intron |
| GCTGTTTCACAGACTTATTC+TGG | 0.239736 | 4.4:-3092343 | MS.gene05909:intron |
| GGTGCATCTGTCCTGGGACT+TGG | 0.253442 | 4.4:-3095047 | MS.gene05909:CDS |
| CCCACAAACCATCTTTATAT+TGG | 0.257952 | 4.4:-3092292 | MS.gene05909:CDS |
| CATCTTGGAATCAATATCTT+TGG | 0.280340 | 4.4:-3095777 | MS.gene05909:intron |
| GTGCATCTGTCCTGGGACTT+GGG | 0.307143 | 4.4:-3095046 | MS.gene05909:CDS |
| CCTTCCCGTACTGATAAGTT+TGG | 0.338870 | 4.4:+3092232 | None:intergenic |
| GATTTATCGTCGACCATAAC+TGG | 0.355353 | 4.4:+3099429 | None:intergenic |
| ATGGTTGGGTGCATCTGTCC+TGG | 0.367570 | 4.4:-3095054 | MS.gene05909:CDS |
| TTTAACTGTCATCTTCTATC+AGG | 0.374360 | 4.4:+3091626 | None:intergenic |
| AATGATGGCTACTTCTTTGA+AGG | 0.378925 | 4.4:+3094680 | None:intergenic |
| GGAGGAAGACGAAGAAGGTT+TGG | 0.385220 | 4.4:+3099329 | None:intergenic |
| GGTTCTTCCTCCTCCAGTTA+TGG | 0.394330 | 4.4:-3099442 | MS.gene05909:CDS |
| GACGCTTGATTCACATGTTC+CGG | 0.398210 | 4.4:-3091663 | MS.gene05909:CDS |
| GATCAAGAAGATGAAGGTTA+TGG | 0.413888 | 4.4:+3095094 | None:intergenic |
| TAACCTTCATCTTCTTGATC+GGG | 0.414790 | 4.4:-3095091 | MS.gene05909:CDS |
| TTTCCAAAGTTGTTCAGAGC+TGG | 0.416693 | 4.4:-3098267 | MS.gene05909:CDS |
| CTGTCCTGGGACTTGGGGAG+TGG | 0.425838 | 4.4:-3095040 | MS.gene05909:CDS |
| TCTCCGATCTATGTTCATCT+TGG | 0.430520 | 4.4:-3095792 | MS.gene05909:CDS |
| ATGTAGAAGGTGATTGCAAT+TGG | 0.430569 | 4.4:+3095852 | None:intergenic |
| TGCCCAGCTCTGAACAACTT+TGG | 0.433303 | 4.4:+3098264 | None:intergenic |
| CCAATATAAAGATGGTTTGT+GGG | 0.434460 | 4.4:+3092292 | None:intergenic |
| TGCATCTGTCCTGGGACTTG+GGG | 0.442253 | 4.4:-3095045 | MS.gene05909:CDS |
| TTCCAAAGTTGTTCAGAGCT+GGG | 0.447509 | 4.4:-3098266 | MS.gene05909:CDS |
| CGAAGAAGGTTTGGAAGCAT+TGG | 0.448650 | 4.4:+3099338 | None:intergenic |
| TTATCAGTACGGGAAGGAAT+TGG | 0.452210 | 4.4:-3092226 | MS.gene05909:intron |
| ATAACCTTCATCTTCTTGAT+CGG | 0.456394 | 4.4:-3095092 | MS.gene05909:CDS |
| GAACCACCAGGTAATGTAGA+AGG | 0.467593 | 4.4:+3095839 | None:intergenic |
| ACCAATATAAAGATGGTTTG+TGG | 0.468348 | 4.4:+3092291 | None:intergenic |
| CAATCACCTTCTACATTACC+TGG | 0.474074 | 4.4:-3095845 | MS.gene05909:CDS |
| TGGTTGGGTGCATCTGTCCT+GGG | 0.488396 | 4.4:-3095053 | MS.gene05909:CDS |
| TGGTCGCCGGCATTAGCGAC+GGG | 0.488768 | 4.4:+3099363 | None:intergenic |
| GTTTGGCCTAATGACATCCT+TGG | 0.502197 | 4.4:+3092249 | None:intergenic |
| CTTATCCCCGTCGCTAATGC+CGG | 0.513099 | 4.4:-3099369 | MS.gene05909:CDS |
| GGAAGCATTGGAGTGGTCGC+CGG | 0.519869 | 4.4:+3099350 | None:intergenic |
| CACACGTGGATGTCGAATGA+TGG | 0.527730 | 4.4:+3094665 | None:intergenic |
| GCCGGCATGATGAACGGAGG+AGG | 0.531869 | 4.4:+3099311 | None:intergenic |
| CGTGTGGGAGAATATGCATT+GGG | 0.534463 | 4.4:-3094648 | MS.gene05909:CDS |
| CTGCCGCTGATGACAGTAGA+GGG | 0.539109 | 4.4:+3099405 | None:intergenic |
| TCCTCCTCCGTTCATCATGC+CGG | 0.539661 | 4.4:-3099312 | MS.gene05909:CDS |
| GTGGTCGCCGGCATTAGCGA+CGG | 0.553074 | 4.4:+3099362 | None:intergenic |
| ATTCCAAGATGAACATAGAT+CGG | 0.557215 | 4.4:+3095789 | None:intergenic |
| TCAACACCAAGGATGTCATT+AGG | 0.557684 | 4.4:-3092255 | MS.gene05909:CDS |
| TTAGGCCAAACTTATCAGTA+CGG | 0.565789 | 4.4:-3092237 | MS.gene05909:CDS |
| ATATATCACCAATATAAAGA+TGG | 0.565838 | 4.4:+3092284 | None:intergenic |
| ACAGACTTATTCTGGAGATG+AGG | 0.566556 | 4.4:-3092335 | MS.gene05909:intron |
| ATTAGTGCTGCGATATCACC+AGG | 0.568343 | 4.4:-3094966 | MS.gene05909:intron |
| TAGGCCAAACTTATCAGTAC+GGG | 0.569426 | 4.4:-3092236 | MS.gene05909:CDS |
| GCAGAAATTGTCGTTTCTGG+AGG | 0.570313 | 4.4:-3091710 | MS.gene05909:intron |
| TGAATCCCTAACCTCACGGC+CGG | 0.572005 | 4.4:+3099293 | None:intergenic |
| TCGTCGACCATAACTGGAGG+AGG | 0.577353 | 4.4:+3099435 | None:intergenic |
| TAAACCACTCCCCAAGTCCC+AGG | 0.578135 | 4.4:+3095036 | None:intergenic |
| AATCCCGATCAAGAAGATGA+AGG | 0.583151 | 4.4:+3095088 | None:intergenic |
| CTCACGGCCGGCATGATGAA+CGG | 0.585801 | 4.4:+3099305 | None:intergenic |
| TCAGGTCTACTTATTTCCAC+CGG | 0.598556 | 4.4:+3091644 | None:intergenic |
| CATGTGAATCAAGCGTCGAT+AGG | 0.608071 | 4.4:+3091669 | None:intergenic |
| ACGGAGGAGGAAGACGAAGA+AGG | 0.610501 | 4.4:+3099324 | None:intergenic |
| CACAAAATGAATGAACCACC+AGG | 0.613316 | 4.4:+3095827 | None:intergenic |
| ATCATTCGACATCCACGTGT+GGG | 0.619745 | 4.4:-3094663 | MS.gene05909:CDS |
| CGTTCATCATGCCGGCCGTG+AGG | 0.624713 | 4.4:-3099304 | MS.gene05909:intron |
| ACGTGTGGGAGAATATGCAT+TGG | 0.630190 | 4.4:-3094649 | MS.gene05909:CDS |
| TTATAGATATGACGAACAAG+AGG | 0.630923 | 4.4:+3095002 | None:intergenic |
| GCCGCTGATGACAGTAGAGG+GGG | 0.637466 | 4.4:+3099407 | None:intergenic |
| CATCATTCGACATCCACGTG+TGG | 0.639962 | 4.4:-3094664 | MS.gene05909:CDS |
| GCTTGATTCACATGTTCCGG+TGG | 0.642011 | 4.4:-3091660 | MS.gene05909:CDS |
| AACCTGAAGGACAACAGACG+AGG | 0.653155 | 4.4:+3094617 | None:intergenic |
| AAGGTTTGGAAGCATTGGAG+TGG | 0.656476 | 4.4:+3099343 | None:intergenic |
| TGCCGCTGATGACAGTAGAG+GGG | 0.658187 | 4.4:+3099406 | None:intergenic |
| TCTGCCGCTGATGACAGTAG+AGG | 0.659168 | 4.4:+3099404 | None:intergenic |
| AATGCATATTCTCCCACACG+TGG | 0.673732 | 4.4:+3094651 | None:intergenic |
| TATATTTCTTGTCAACACCA+AGG | 0.675625 | 4.4:-3092266 | MS.gene05909:CDS |
| GGTCGCCGGCATTAGCGACG+GGG | 0.686093 | 4.4:+3099364 | None:intergenic |
| TCAAGCGTCGATAGGATCTG+CGG | 0.688220 | 4.4:+3091677 | None:intergenic |
| ACGGCCGGCATGATGAACGG+AGG | 0.688570 | 4.4:+3099308 | None:intergenic |
| CCAAACTTATCAGTACGGGA+AGG | 0.693328 | 4.4:-3092232 | MS.gene05909:CDS |
| TCACCTTCTACATTACCTGG+TGG | 0.710584 | 4.4:-3095842 | MS.gene05909:CDS |
| TTATCGTCGACCATAACTGG+AGG | 0.712922 | 4.4:+3099432 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 3091634 | 3099466 | 3091634 | ID=MS.gene05909 |
| chr4.4 | mRNA | 3091634 | 3099466 | 3091634 | ID=MS.gene05909.t1;Parent=MS.gene05909 |
| chr4.4 | exon | 3099305 | 3099466 | 3099305 | ID=MS.gene05909.t1.exon1;Parent=MS.gene05909.t1 |
| chr4.4 | CDS | 3099305 | 3099466 | 3099305 | ID=cds.MS.gene05909.t1;Parent=MS.gene05909.t1 |
| chr4.4 | exon | 3098241 | 3098293 | 3098241 | ID=MS.gene05909.t1.exon2;Parent=MS.gene05909.t1 |
| chr4.4 | CDS | 3098241 | 3098293 | 3098241 | ID=cds.MS.gene05909.t1;Parent=MS.gene05909.t1 |
| chr4.4 | exon | 3095778 | 3095887 | 3095778 | ID=MS.gene05909.t1.exon3;Parent=MS.gene05909.t1 |
| chr4.4 | CDS | 3095778 | 3095887 | 3095778 | ID=cds.MS.gene05909.t1;Parent=MS.gene05909.t1 |
| chr4.4 | exon | 3094967 | 3095134 | 3094967 | ID=MS.gene05909.t1.exon4;Parent=MS.gene05909.t1 |
| chr4.4 | CDS | 3094967 | 3095134 | 3094967 | ID=cds.MS.gene05909.t1;Parent=MS.gene05909.t1 |
| chr4.4 | exon | 3094620 | 3094717 | 3094620 | ID=MS.gene05909.t1.exon5;Parent=MS.gene05909.t1 |
| chr4.4 | CDS | 3094620 | 3094717 | 3094620 | ID=cds.MS.gene05909.t1;Parent=MS.gene05909.t1 |
| chr4.4 | exon | 3092227 | 3092353 | 3092227 | ID=MS.gene05909.t1.exon6;Parent=MS.gene05909.t1 |
| chr4.4 | CDS | 3092227 | 3092353 | 3092227 | ID=cds.MS.gene05909.t1;Parent=MS.gene05909.t1 |
| chr4.4 | exon | 3091634 | 3091728 | 3091634 | ID=MS.gene05909.t1.exon7;Parent=MS.gene05909.t1 |
| chr4.4 | CDS | 3091634 | 3091728 | 3091634 | ID=cds.MS.gene05909.t1;Parent=MS.gene05909.t1 |
| Gene Sequence |
| Protein sequence |
