AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059482

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059482.t1 MTR_1g008990 98.780 82 1 0 1 82 249 330 2.87e-54 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCAATTGACTCTGATTT+TGG 0.206784 1.3:-11123688 None:intergenic
CAGAATTCAGCTATTGGACT+TGG 0.407633 1.3:+11123812 MS.gene059482:CDS
AGTGGACAGAATTCAGCTAT+TGG 0.421178 1.3:+11123806 MS.gene059482:CDS
TTTCCATGGACGGTGATTGT+AGG 0.427364 1.3:-11123764 None:intergenic
CATGGAAACGACGCTTCATA+TGG 0.432634 1.3:+11123779 MS.gene059482:CDS
CACTAGTAATCTCCGTTGTA+TGG 0.434010 1.3:+11123730 MS.gene059482:CDS
CATATGAAGCGTCGTTTCCA+TGG 0.454343 1.3:-11123778 None:intergenic
GACGCTTCATATGGTGTTAG+TGG 0.460901 1.3:+11123788 MS.gene059482:CDS
AGCAGAGGATATGGTCTCTA+GGG 0.483549 1.3:+11123634 None:intergenic
AGATTACTAGTGTTGATTTG+TGG 0.491316 1.3:-11123719 None:intergenic
TAGCAGAGGATATGGTCTCT+AGG 0.512033 1.3:+11123633 None:intergenic
AGAAGCTGGTTGTTAAAAGT+AGG 0.514450 1.3:-11123665 None:intergenic
TGCAGTCATGATTTCAACAA+TGG 0.523773 1.3:+11123851 MS.gene059482:CDS
TCGCCTACAATCACCGTCCA+TGG 0.536280 1.3:+11123761 MS.gene059482:CDS
TGAAGCGTCGTTTCCATGGA+CGG 0.541190 1.3:-11123774 None:intergenic
TTACTAGTGTTGATTTGTGG+CGG 0.621086 1.3:-11123716 None:intergenic
GCGAAACATGCGCCATACAA+CGG 0.641264 1.3:-11123742 None:intergenic
AGTGTTGATTTGTGGCGGAG+TGG 0.681459 1.3:-11123711 None:intergenic
GCAGAGGATATGGTCTCTAG+GGG 0.691454 1.3:+11123635 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATTACTAGTGTTGATTTG+TGG - chr1.3:11123722-11123741 None:intergenic 30.0%
!! TGTTCAATTGACTCTGATTT+TGG - chr1.3:11123691-11123710 None:intergenic 30.0%
AGAAGCTGGTTGTTAAAAGT+AGG - chr1.3:11123668-11123687 None:intergenic 35.0%
TGCAGTCATGATTTCAACAA+TGG + chr1.3:11123851-11123870 MS.gene059482:CDS 35.0%
!! TTACTAGTGTTGATTTGTGG+CGG - chr1.3:11123719-11123738 None:intergenic 35.0%
AGTGGACAGAATTCAGCTAT+TGG + chr1.3:11123806-11123825 MS.gene059482:CDS 40.0%
CACTAGTAATCTCCGTTGTA+TGG + chr1.3:11123730-11123749 MS.gene059482:CDS 40.0%
CAGAATTCAGCTATTGGACT+TGG + chr1.3:11123812-11123831 MS.gene059482:CDS 40.0%
CATGGAAACGACGCTTCATA+TGG + chr1.3:11123779-11123798 MS.gene059482:CDS 45.0%
TTTCCATGGACGGTGATTGT+AGG - chr1.3:11123767-11123786 None:intergenic 45.0%
! GACGCTTCATATGGTGTTAG+TGG + chr1.3:11123788-11123807 MS.gene059482:CDS 45.0%
!! CATATGAAGCGTCGTTTCCA+TGG - chr1.3:11123781-11123800 None:intergenic 45.0%
!! GACTCTGATTTTGGAGAAGC+TGG - chr1.3:11123682-11123701 None:intergenic 45.0%
GCGAAACATGCGCCATACAA+CGG - chr1.3:11123745-11123764 None:intergenic 50.0%
!! AGTGTTGATTTGTGGCGGAG+TGG - chr1.3:11123714-11123733 None:intergenic 50.0%
!! TGAAGCGTCGTTTCCATGGA+CGG - chr1.3:11123777-11123796 None:intergenic 50.0%
TCGCCTACAATCACCGTCCA+TGG + chr1.3:11123761-11123780 MS.gene059482:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 11123644 11123889 11123644 ID=MS.gene059482
chr1.3 mRNA 11123644 11123889 11123644 ID=MS.gene059482.t1;Parent=MS.gene059482
chr1.3 exon 11123644 11123889 11123644 ID=MS.gene059482.t1.exon1;Parent=MS.gene059482.t1
chr1.3 CDS 11123644 11123889 11123644 ID=cds.MS.gene059482.t1;Parent=MS.gene059482.t1
Gene Sequence

>MS.gene059482

ATGGTCTCTAGGGGTACTTTACCTACTTTTAACAACCAGCTTCTCCAAAATCAGAGTCAATTGAACACCACTCCGCCACAAATCAACACTAGTAATCTCCGTTGTATGGCGCATGTTTCGCCTACAATCACCGTCCATGGAAACGACGCTTCATATGGTGTTAGTGGACAGAATTCAGCTATTGGACTTGGAGATTTTGATATATCTTGCAGTCATGATTTCAACAATGGAGTTAATAGTGATGCT
Protein sequence

>MS.gene059482.t1

MVSRGTLPTFNNQLLQNQSQLNTTPPQINTSNLRCMAHVSPTITVHGNDASYGVSGQNSAIGLGDFDISCSHDFNNGVNSDA