Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059483.t1 | XP_003588595.1 | 98.9 | 90 | 1 | 0 | 1 | 90 | 249 | 338 | 9.20E-42 | 179.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059483.t1 | G7I545 | 98.9 | 90 | 1 | 0 | 1 | 90 | 249 | 338 | 6.7e-42 | 179.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059104 | MS.gene059483 | 0.825633 | 4.21E-54 | -1.69E-46 |
| MS.gene059482 | MS.gene059483 | 0.989502 | 2.13E-178 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059483.t1 | MTR_1g008990 | 98.889 | 90 | 1 | 0 | 1 | 90 | 249 | 338 | 2.92e-60 | 187 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTCAATTGACTCTGATTT+TGG | 0.206784 | 1.3:-11135257 | None:intergenic |
| TGAGCTCTTTGACTAGCTTA+TGG | 0.345147 | 1.3:+11135460 | MS.gene059483:CDS |
| CAGAATTCAGCTATTGGACT+TGG | 0.407633 | 1.3:+11135381 | MS.gene059483:CDS |
| AGTGGACAGAATTCAGCTAT+TGG | 0.421178 | 1.3:+11135375 | MS.gene059483:CDS |
| TTTCCATGGACGGTGATTGT+AGG | 0.427364 | 1.3:-11135333 | None:intergenic |
| CATGGAAACGACGCTTCATA+TGG | 0.432634 | 1.3:+11135348 | MS.gene059483:CDS |
| CACTAGTAATCTCCGTTGTA+TGG | 0.434010 | 1.3:+11135299 | MS.gene059483:CDS |
| CATATGAAGCGTCGTTTCCA+TGG | 0.454343 | 1.3:-11135347 | None:intergenic |
| GACGCTTCATATGGTGTTAG+TGG | 0.460901 | 1.3:+11135357 | MS.gene059483:CDS |
| AGCAGAGGATATGGTCTCTA+GGG | 0.483549 | 1.3:+11135203 | None:intergenic |
| AGATTACTAGTGTTGATTTG+TGG | 0.491316 | 1.3:-11135288 | None:intergenic |
| TAGCAGAGGATATGGTCTCT+AGG | 0.512033 | 1.3:+11135202 | None:intergenic |
| AGAAGCTGGTTGTTAAAAGT+AGG | 0.514450 | 1.3:-11135234 | None:intergenic |
| TGCAGTCATGATTTCAACAA+TGG | 0.523773 | 1.3:+11135420 | MS.gene059483:CDS |
| TCGCCTACAATCACCGTCCA+TGG | 0.536280 | 1.3:+11135330 | MS.gene059483:CDS |
| TGAAGCGTCGTTTCCATGGA+CGG | 0.541190 | 1.3:-11135343 | None:intergenic |
| TTACTAGTGTTGATTTGTGG+CGG | 0.621086 | 1.3:-11135285 | None:intergenic |
| GCGAAACATGCGCCATACAA+CGG | 0.641264 | 1.3:-11135311 | None:intergenic |
| AGTGTTGATTTGTGGCGGAG+TGG | 0.681459 | 1.3:-11135280 | None:intergenic |
| GCAGAGGATATGGTCTCTAG+GGG | 0.691454 | 1.3:+11135204 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATTACTAGTGTTGATTTG+TGG | - | chr1.3:11135291-11135310 | None:intergenic | 30.0% |
| !! | TGTTCAATTGACTCTGATTT+TGG | - | chr1.3:11135260-11135279 | None:intergenic | 30.0% |
| AGAAGCTGGTTGTTAAAAGT+AGG | - | chr1.3:11135237-11135256 | None:intergenic | 35.0% | |
| TGCAGTCATGATTTCAACAA+TGG | + | chr1.3:11135420-11135439 | MS.gene059483:CDS | 35.0% | |
| !! | TTACTAGTGTTGATTTGTGG+CGG | - | chr1.3:11135288-11135307 | None:intergenic | 35.0% |
| AGTGGACAGAATTCAGCTAT+TGG | + | chr1.3:11135375-11135394 | MS.gene059483:CDS | 40.0% | |
| CACTAGTAATCTCCGTTGTA+TGG | + | chr1.3:11135299-11135318 | MS.gene059483:CDS | 40.0% | |
| CAGAATTCAGCTATTGGACT+TGG | + | chr1.3:11135381-11135400 | MS.gene059483:CDS | 40.0% | |
| !! | TGAGCTCTTTGACTAGCTTA+TGG | + | chr1.3:11135460-11135479 | MS.gene059483:CDS | 40.0% |
| CATGGAAACGACGCTTCATA+TGG | + | chr1.3:11135348-11135367 | MS.gene059483:CDS | 45.0% | |
| TTTCCATGGACGGTGATTGT+AGG | - | chr1.3:11135336-11135355 | None:intergenic | 45.0% | |
| ! | GACGCTTCATATGGTGTTAG+TGG | + | chr1.3:11135357-11135376 | MS.gene059483:CDS | 45.0% |
| !! | CATATGAAGCGTCGTTTCCA+TGG | - | chr1.3:11135350-11135369 | None:intergenic | 45.0% |
| !! | GACTCTGATTTTGGAGAAGC+TGG | - | chr1.3:11135251-11135270 | None:intergenic | 45.0% |
| GCGAAACATGCGCCATACAA+CGG | - | chr1.3:11135314-11135333 | None:intergenic | 50.0% | |
| !! | AGTGTTGATTTGTGGCGGAG+TGG | - | chr1.3:11135283-11135302 | None:intergenic | 50.0% |
| !! | TGAAGCGTCGTTTCCATGGA+CGG | - | chr1.3:11135346-11135365 | None:intergenic | 50.0% |
| TCGCCTACAATCACCGTCCA+TGG | + | chr1.3:11135330-11135349 | MS.gene059483:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 11135213 | 11135488 | 11135213 | ID=MS.gene059483 |
| chr1.3 | mRNA | 11135213 | 11135488 | 11135213 | ID=MS.gene059483.t1;Parent=MS.gene059483 |
| chr1.3 | exon | 11135213 | 11135488 | 11135213 | ID=MS.gene059483.t1.exon1;Parent=MS.gene059483.t1 |
| chr1.3 | CDS | 11135213 | 11135488 | 11135213 | ID=cds.MS.gene059483.t1;Parent=MS.gene059483.t1 |
| Gene Sequence |
| Protein sequence |
