AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059615

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059615.t1 MTR_6g087990 100.000 162 0 0 1 162 1 162 2.82e-116 326
MS.gene059615.t1 MTR_7g103610 55.828 163 70 1 2 162 55 217 4.25e-63 193
MS.gene059615.t1 MTR_7g103610 56.081 148 63 1 2 147 55 202 8.57e-56 175
MS.gene059615.t1 MTR_2g022660 42.446 139 79 1 22 159 58 196 5.82e-33 116
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059615.t1 AT1G65980 73.457 162 43 0 1 162 1 162 3.92e-92 265
MS.gene059615.t1 AT1G65970 72.222 162 45 0 1 162 1 162 1.96e-90 260
MS.gene059615.t1 AT1G60740 71.605 162 46 0 1 162 1 162 1.98e-89 258
MS.gene059615.t1 AT3G52960 56.098 164 69 2 2 162 71 234 3.99e-61 189
MS.gene059615.t1 AT1G65990 58.553 152 59 1 1 152 1 148 3.72e-59 192
MS.gene059615.t1 AT1G65980 73.077 78 21 0 1 78 1 78 1.46e-39 129
MS.gene059615.t1 AT3G06050 46.032 126 67 1 35 159 73 198 1.61e-35 122
MS.gene059615.t1 AT3G06050 53.571 56 26 0 35 90 73 128 1.49e-18 78.6

Find 48 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCATCATCTTTGCTGTTCC+TGG 0.271985 6.2:-87388555 MS.gene059615:CDS
CCCAAGTGCATGTGTGTATT+TGG 0.291016 6.2:+87386764 None:intergenic
CATTTGCAACCTTCACCTTC+AGG 0.298861 6.2:+87386673 None:intergenic
GTGCCTTCACACCCACTTGC+AGG 0.324438 6.2:-87388533 MS.gene059615:intron
AACAGCTTGAAGCATGTTCC+TGG 0.350575 6.2:-87387667 MS.gene059615:intron
ATCACATCTCCTACTTTGAT+TGG 0.352692 6.2:+87388660 None:intergenic
GCCAAATACACACATGCACT+TGG 0.384360 6.2:-87386765 MS.gene059615:CDS
AGGAAGGCTAAGGTGCCATC+TGG 0.393148 6.2:+87388636 None:intergenic
ATCCATTTGTGCTGAATTCA+TGG 0.400960 6.2:-87386839 MS.gene059615:CDS
AACTTGACATGCTTGTTCTC+CGG 0.405881 6.2:+87386804 None:intergenic
GCCCATGAATTCAGCACAAA+TGG 0.408653 6.2:+87386837 None:intergenic
CTTGTTCTCCGGGAATGTCT+TGG 0.410062 6.2:+87386815 None:intergenic
ACTTGACATGCTTGTTCTCC+GGG 0.418105 6.2:+87386805 None:intergenic
GAATGAATGGTGACAGATTG+AGG 0.441168 6.2:+87388597 None:intergenic
AACAAGAAGAGCAAACCTCT+TGG 0.455187 6.2:+87386701 None:intergenic
CTTGATGATTTCTTCAGCAC+CGG 0.493646 6.2:+87386623 None:intergenic
GCCGGCAGAGAGGGAATGAA+TGG 0.496618 6.2:+87388584 None:intergenic
CAAGTGGGTGTGAAGGCACC+AGG 0.499182 6.2:+87388537 None:intergenic
TTCTTCATCAAGGAAGGCTA+AGG 0.511511 6.2:+87388626 None:intergenic
GACCTCAGCGACAAAGGTCT+CGG 0.528430 6.2:-87386732 MS.gene059615:CDS
GGAGGAGAATACACCATCTC+CGG 0.528627 6.2:-87386642 MS.gene059615:CDS
CATGTCAAGTTCCTTGCTGA+TGG 0.530762 6.2:-87386792 MS.gene059615:CDS
TCAGAAGAGCTGAAAGGAAA+GGG 0.536940 6.2:-87387631 MS.gene059615:CDS
TTCATGGGCCAAGACATTCC+CGG 0.539512 6.2:-87386823 MS.gene059615:CDS
GATTTCTTCAGCACCGGAGA+TGG 0.540136 6.2:+87386629 None:intergenic
TCTGATCTCTCAATGAAGCC+AGG 0.542391 6.2:+87387649 None:intergenic
GGAAGGCTAAGGTGCCATCT+GGG 0.546906 6.2:+87388637 None:intergenic
GAGCTTGACCTCAGCGACAA+AGG 0.549694 6.2:-87386738 MS.gene059615:CDS
TCAATGGCTCCAATCAAAGT+AGG 0.550950 6.2:-87388669 MS.gene059615:CDS
ACCATTCATTCCCTCTCTGC+CGG 0.561297 6.2:-87388585 MS.gene059615:CDS
ATACACACATGCACTTGGGT+TGG 0.561559 6.2:-87386760 MS.gene059615:CDS
AAGGTTGCAAATGTTGAAGA+AGG 0.566267 6.2:-87386663 MS.gene059615:CDS
TCTTCTTGTTGAAGACCTGA+AGG 0.568574 6.2:-87386688 MS.gene059615:CDS
ATCAGAAGAGCTGAAAGGAA+AGG 0.571012 6.2:-87387632 MS.gene059615:CDS
GTTGCAAATGTTGAAGAAGG+AGG 0.581974 6.2:-87386660 MS.gene059615:CDS
TGCCGAGACCTTTGTCGCTG+AGG 0.592538 6.2:+87386730 None:intergenic
CGGTGCTGAAGAAATCATCA+AGG 0.594261 6.2:-87386622 MS.gene059615:CDS
CCAAATACACACATGCACTT+GGG 0.598201 6.2:-87386764 MS.gene059615:CDS
TAACCTGCAAGTGGGTGTGA+AGG 0.601313 6.2:+87388530 None:intergenic
TCCATTTGTGCTGAATTCAT+GGG 0.611814 6.2:-87386838 MS.gene059615:CDS
GAGAGATCAGAAGAGCTGAA+AGG 0.621848 6.2:-87387637 MS.gene059615:CDS
AAAAGAGGTTAACCTGCAAG+TGG 0.626645 6.2:+87388521 None:intergenic
GTAGGAGATGTGATCCCAGA+TGG 0.630920 6.2:-87388651 MS.gene059615:CDS
TGTTGAAGACCTGAAGGTGA+AGG 0.633342 6.2:-87386682 MS.gene059615:CDS
GTCTCGGCATTCGCTCCAAG+AGG 0.644758 6.2:-87386716 MS.gene059615:CDS
GATGAAATCATCTGCATCAG+TGG 0.655212 6.2:-87387604 MS.gene059615:intron
AAAGAGGTTAACCTGCAAGT+GGG 0.674981 6.2:+87388522 None:intergenic
ATTTGGCAGAACCATCAGCA+AGG 0.715154 6.2:+87386781 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTCTTTTGTATAAAATT+CGG - chr6.2:87387442-87387461 MS.gene059615:intron 10.0%
!! AAATTCAAATTGCTTATAAT+CGG + chr6.2:87388324-87388343 None:intergenic 15.0%
!! GTGAAAAATAAATAAATCAA+AGG + chr6.2:87387094-87387113 None:intergenic 15.0%
!!! GGAATATTTTTTTTATAATG+TGG - chr6.2:87388113-87388132 MS.gene059615:intron 15.0%
!!! TATAAAATTCGGAAATTTTA+AGG - chr6.2:87387453-87387472 MS.gene059615:intron 15.0%
!!! TCTAATTATGTTATTTTGTT+TGG - chr6.2:87387590-87387609 MS.gene059615:intron 15.0%
!!! TTTTGATTTTATTTATGTGT+TGG - chr6.2:87387290-87387309 MS.gene059615:intron 15.0%
!! GTGAAAAACAAATAAATCAA+AGG + chr6.2:87386930-87386949 None:intergenic 20.0%
!!! ATCTATGAGAATTTTGAAAT+GGG - chr6.2:87387373-87387392 MS.gene059615:intron 20.0%
! ATAAAATTGAATCAGATCCA+TGG - chr6.2:87386819-87386838 MS.gene059615:CDS 25.0%
! CAACAACAAAACAATCATTA+TGG + chr6.2:87387851-87387870 None:intergenic 25.0%
! TAAAATTGAATCAGATCCAT+GGG - chr6.2:87386820-87386839 MS.gene059615:CDS 25.0%
!! ATTAGACTGAATTAGTTTCA+TGG - chr6.2:87386983-87387002 MS.gene059615:intron 25.0%
!! TTAGACTGAATTAGTTTCAT+GGG - chr6.2:87386984-87387003 MS.gene059615:intron 25.0%
!!! GATCTATGAGAATTTTGAAA+TGG - chr6.2:87387372-87387391 MS.gene059615:intron 25.0%
!!! GTTTTGTTTCACTTTTTACA+AGG - chr6.2:87387519-87387538 MS.gene059615:intron 25.0%
!!! TTTTTAGGGTTAAGATTTGA+AGG + chr6.2:87386795-87386814 None:intergenic 25.0%
AATTACGAAGCTTGAAATCT+AGG + chr6.2:87388245-87388264 None:intergenic 30.0%
ACAAGGTTGTCTTGATAAAA+AGG - chr6.2:87387536-87387555 MS.gene059615:intron 30.0%
CAAGGTTGTCTTGATAAAAA+GGG - chr6.2:87387537-87387556 MS.gene059615:intron 30.0%
CATATTCAATTCAAGAACCA+GGG - chr6.2:87387930-87387949 MS.gene059615:intron 30.0%
GAAGGAAAATTGATGAAAAG+AGG + chr6.2:87386777-87386796 None:intergenic 30.0%
GATTCAATGTCTTGCTTAAA+TGG - chr6.2:87388072-87388091 MS.gene059615:intron 30.0%
TCATATTCAATTCAAGAACC+AGG - chr6.2:87387929-87387948 MS.gene059615:intron 30.0%
TGAAAAGCTGCATTAAAATC+TGG - chr6.2:87388281-87388300 MS.gene059615:intron 30.0%
TTTGTCATATAGATACCTGT+TGG - chr6.2:87388204-87388223 MS.gene059615:intron 30.0%
! ATTAGACTGAATTAGCTTCA+TGG - chr6.2:87386901-87386920 MS.gene059615:intron 30.0%
! TTAGACTGAATTAGCTTCAT+GGG - chr6.2:87386902-87386921 MS.gene059615:intron 30.0%
!! GAAATTTTAAGGATACTCTG+AGG - chr6.2:87387464-87387483 MS.gene059615:intron 30.0%
!! TTTGTTTTCTTCATCAAGGA+AGG + chr6.2:87386663-87386682 None:intergenic 30.0%
!!! AAAGATGATGACTTTTTTGC+CGG + chr6.2:87386717-87386736 None:intergenic 30.0%
AAGGTTGCAAATGTTGAAGA+AGG - chr6.2:87388617-87388636 MS.gene059615:CDS 35.0%
ATCACATCTCCTACTTTGAT+TGG + chr6.2:87386623-87386642 None:intergenic 35.0%
ATCCATTTGTGCTGAATTCA+TGG - chr6.2:87388441-87388460 MS.gene059615:intron 35.0%
GTCTTGCTTAAATGGTGATA+TGG - chr6.2:87388080-87388099 MS.gene059615:intron 35.0%
TCCATTTGTGCTGAATTCAT+GGG - chr6.2:87388442-87388461 MS.gene059615:intron 35.0%
!! GAGGTTTGTTTTCTTCATCA+AGG + chr6.2:87386667-87386686 None:intergenic 35.0%
AAAAGAGGTTAACCTGCAAG+TGG + chr6.2:87386762-87386781 None:intergenic 40.0%
AAAGAGGTTAACCTGCAAGT+GGG + chr6.2:87386761-87386780 None:intergenic 40.0%
AACAAGAAGAGCAAACCTCT+TGG + chr6.2:87388582-87388601 None:intergenic 40.0%
AACTTGACATGCTTGTTCTC+CGG + chr6.2:87388479-87388498 None:intergenic 40.0%
ACAAATCAAGTAACAAGCCC+TGG + chr6.2:87387950-87387969 None:intergenic 40.0%
ATCAGAAGAGCTGAAAGGAA+AGG - chr6.2:87387648-87387667 MS.gene059615:CDS 40.0%
CAGCATCATAAGTAGAGAAC+AGG + chr6.2:87388368-87388387 None:intergenic 40.0%
CCAAATACACACATGCACTT+GGG - chr6.2:87388516-87388535 MS.gene059615:intron 40.0%
CTGTTCTCTACTTATGATGC+TGG - chr6.2:87388366-87388385 MS.gene059615:intron 40.0%
GAATGAATGGTGACAGATTG+AGG + chr6.2:87386686-87386705 None:intergenic 40.0%
GATGAAATCATCTGCATCAG+TGG - chr6.2:87387676-87387695 MS.gene059615:intron 40.0%
GTTGCAAATGTTGAAGAAGG+AGG - chr6.2:87388620-87388639 MS.gene059615:CDS 40.0%
TAACACAGTGTATTCGCCAA+TGG + chr6.2:87388159-87388178 None:intergenic 40.0%
TCAGAAGAGCTGAAAGGAAA+GGG - chr6.2:87387649-87387668 MS.gene059615:CDS 40.0%
TCTTCTTGTTGAAGACCTGA+AGG - chr6.2:87388592-87388611 MS.gene059615:CDS 40.0%
TTCTTCATCAAGGAAGGCTA+AGG + chr6.2:87386657-87386676 None:intergenic 40.0%
! CTTGATGATTTCTTCAGCAC+CGG + chr6.2:87388660-87388679 None:intergenic 40.0%
! TAAGGATACTCTGAGGATAG+AGG - chr6.2:87387471-87387490 MS.gene059615:intron 40.0%
! TGGTGATATGGATTGAAGCA+TGG - chr6.2:87388092-87388111 MS.gene059615:intron 40.0%
AACAGCTTGAAGCATGTTCC+TGG - chr6.2:87387613-87387632 MS.gene059615:CDS 45.0%
ACTTGACATGCTTGTTCTCC+GGG + chr6.2:87388478-87388497 None:intergenic 45.0%
ATACACACATGCACTTGGGT+TGG - chr6.2:87388520-87388539 MS.gene059615:intron 45.0%
ATTTGGCAGAACCATCAGCA+AGG + chr6.2:87388502-87388521 None:intergenic 45.0%
CATGTCAAGTTCCTTGCTGA+TGG - chr6.2:87388488-87388507 MS.gene059615:intron 45.0%
CATTTGCAACCTTCACCTTC+AGG + chr6.2:87388610-87388629 None:intergenic 45.0%
CCCAAGTGCATGTGTGTATT+TGG + chr6.2:87388519-87388538 None:intergenic 45.0%
CTGAGGATAGAGGAACATCA+TGG - chr6.2:87387481-87387500 MS.gene059615:intron 45.0%
GAGAGATCAGAAGAGCTGAA+AGG - chr6.2:87387643-87387662 MS.gene059615:CDS 45.0%
GATACCTGTTGGATCGAGAT+TGG - chr6.2:87388215-87388234 MS.gene059615:intron 45.0%
GCCAAATACACACATGCACT+TGG - chr6.2:87388515-87388534 MS.gene059615:intron 45.0%
GCCCATGAATTCAGCACAAA+TGG + chr6.2:87388446-87388465 None:intergenic 45.0%
TAGTCCAATCTCGATCCAAC+AGG + chr6.2:87388222-87388241 None:intergenic 45.0%
TCTGATCTCTCAATGAAGCC+AGG + chr6.2:87387634-87387653 None:intergenic 45.0%
TGTTGAAGACCTGAAGGTGA+AGG - chr6.2:87388598-87388617 MS.gene059615:CDS 45.0%
! CGGTGCTGAAGAAATCATCA+AGG - chr6.2:87388658-87388677 MS.gene059615:CDS 45.0%
!! CGATGAATCAAAAGTGCCCA+TGG + chr6.2:87386839-87386858 None:intergenic 45.0%
!! GTCATCATCTTTGCTGTTCC+TGG - chr6.2:87386725-87386744 MS.gene059615:CDS 45.0%
!!! TCAATTTTATTTTATTTTTA+GGG + chr6.2:87386809-87386828 None:intergenic 5.0%
!!! TTCAATTTTATTTTATTTTT+AGG + chr6.2:87386810-87386829 None:intergenic 5.0%
AACTGCAAGCTTCGTGCCAT+TGG - chr6.2:87388140-87388159 MS.gene059615:intron 50.0%
ACCATTCATTCCCTCTCTGC+CGG - chr6.2:87386695-87386714 MS.gene059615:CDS 50.0%
CTTGTTCTCCGGGAATGTCT+TGG + chr6.2:87388468-87388487 None:intergenic 50.0%
GGAGGAGAATACACCATCTC+CGG - chr6.2:87388638-87388657 MS.gene059615:CDS 50.0%
GTAGGAGATGTGATCCCAGA+TGG - chr6.2:87386629-87386648 MS.gene059615:CDS 50.0%
TAACCTGCAAGTGGGTGTGA+AGG + chr6.2:87386753-87386772 None:intergenic 50.0%
TTCATGGGCCAAGACATTCC+CGG - chr6.2:87388457-87388476 MS.gene059615:intron 50.0%
! GATTTCTTCAGCACCGGAGA+TGG + chr6.2:87388654-87388673 None:intergenic 50.0%
!!! GACTTTTTTGCCGGCAGAGA+GGG + chr6.2:87386708-87386727 None:intergenic 50.0%
!!! TGACTTTTTTGCCGGCAGAG+AGG + chr6.2:87386709-87386728 None:intergenic 50.0%
GACCTCAGCGACAAAGGTCT+CGG - chr6.2:87388548-87388567 MS.gene059615:CDS 55.0%
GAGCTTGACCTCAGCGACAA+AGG - chr6.2:87388542-87388561 MS.gene059615:CDS 55.0%
! AGGAAGGCTAAGGTGCCATC+TGG + chr6.2:87386647-87386666 None:intergenic 55.0%
! GGAAGGCTAAGGTGCCATCT+GGG + chr6.2:87386646-87386665 None:intergenic 55.0%
GCCGGCAGAGAGGGAATGAA+TGG + chr6.2:87386699-87386718 None:intergenic 60.0%
GTCTCGGCATTCGCTCCAAG+AGG - chr6.2:87388564-87388583 MS.gene059615:CDS 60.0%
GTGCCTTCACACCCACTTGC+AGG - chr6.2:87386747-87386766 MS.gene059615:CDS 60.0%
TGCCGAGACCTTTGTCGCTG+AGG + chr6.2:87388553-87388572 None:intergenic 60.0%
!! CAAGTGGGTGTGAAGGCACC+AGG + chr6.2:87386746-87386765 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 87386614 87388688 87386614 ID=MS.gene059615
chr6.2 mRNA 87386614 87388688 87386614 ID=MS.gene059615.t1;Parent=MS.gene059615
chr6.2 exon 87388534 87388688 87388534 ID=MS.gene059615.t1.exon1;Parent=MS.gene059615.t1
chr6.2 CDS 87388534 87388688 87388534 ID=cds.MS.gene059615.t1;Parent=MS.gene059615.t1
chr6.2 exon 87387605 87387684 87387605 ID=MS.gene059615.t1.exon2;Parent=MS.gene059615.t1
chr6.2 CDS 87387605 87387684 87387605 ID=cds.MS.gene059615.t1;Parent=MS.gene059615.t1
chr6.2 exon 87386614 87386867 87386614 ID=MS.gene059615.t1.exon3;Parent=MS.gene059615.t1
chr6.2 CDS 87386614 87386867 87386614 ID=cds.MS.gene059615.t1;Parent=MS.gene059615.t1
Gene Sequence

>MS.gene059615

ATGGCTCCAATCAAAGTAGGAGATGTGATCCCAGATGGCACCTTAGCCTTCCTTGATGAAGAAAACAAACCTCAATCTGTCACCATTCATTCCCTCTCTGCCGGCAAAAAAGTCATCATCTTTGCTGTTCCTGGTGCCTTCACACCCACTTGCAGGTTAACCTCTTTTCATCAATTTTCCTTCAAATCTTAACCCTAAAAATAAAATAAAATTGAATCAGATCCATGGGCACTTTTGATTCATCGTTTTTTATCTTAAAGTTTTCATTTTTATTTGATTTATGTGTTATTAGACTGAATTAGCTTCATGGGTACCTTTGATTTATTTGTTTTTCACGTAAAGTTTTGATTTTTATTTGATTTACGTGTTATTAGACTGAATTAGTTTCATGGGTACCTTTGATTTATTTGTTTTTCACGTAAAGTTTTGATTTTTATTTGATTTTCGTGTTATTAGACTGAATTAGCTTCATGGGTACCTTTGATTTATTTATTTTTCACGTAAAGTTTTGATTTTTATTTGATTTACGTGTTATTAGACTGAATTAGCTTCATGGGTACCTTTGATTTATTTGTTTTTCACGTAAAGTTTTGATTTTTATTTGATTTACGTGTTATTAGACTGAATTAGCTTCATGGGTACCTTTGATTTATTTGTTTTTCACTTAAAGTTTTGATTTTGATTTTATTTATGTGTTGGTTAAAGTTCATTTTTATTTGATTTATGTGTTATTTGTTCATATGAGATTTTACTACTGTGATCTATGAGAATTTTGAAATGGGTCACTCAAATTGTCTTATTTTTATTGTCTCAAATTGTCTATGAGAATTTTTCTTTTGTATAAAATTCGGAAATTTTAAGGATACTCTGAGGATAGAGGAACATCATGGTTGTGAGATGTTTTTGTTTTGTTTCACTTTTTACAAGGTTGTCTTGATAAAAAGGGTTATGTTTCAAGATGTTATTCAATTTGAGATCTAATTATGTTATTTTGTTTGGAACAGCTTGAAGCATGTTCCTGGCTTCATTGAGAGATCAGAAGAGCTGAAAGGAAAGGGTGTTGATGAAATCATCTGCATCAGTGGTAAAGTTTCTAGTCATATATCATGATTTTGTTATCTATAATGATAATGATTGTTTTGTTGCTGATTGTGTTGTTTATTGACAAATATTATATGTACACGTAGTTCTTAGTTCTTAGTTTTTACAACTTTCATGTTCAATTCAACGTTATCCATAATGATTGTTTTGTTGTTGATTGTGTTGTTCATTGACAAATATTATATGTACACGTAGTTCTTAGTTTTTACAACTTTCATATTCAATTCAAGAACCAGGGCTTGTTACTTGATTTGTATTGTATTTGCTGATTATTGTTTGTTGATTTGTTTTACTTCAATGATTTGATTTTTGTAGTTGTTTATCTTATAATTTAGATTGAACATGTCTTAGATTTTCGATTCAATGTCTTGCTTAAATGGTGATATGGATTGAAGCATGGAATATTTTTTTTATAATGTGGATCTAACTGCAAGCTTCGTGCCATTGGCGAATACACTGTGTTAACGTCAAAGAATTCACATTCACGCATTTGTCATATAGATACCTGTTGGATCGAGATTGGACTACCTAGATTTCAAGCTTCGTAATTTAGTTTTTTAAAATGATGAAAAGCTGCATTAAAATCTGGACTGTTTACTAATGTTACCGATTATAAGCAATTTGAATTTGCTAACATAATAACATGAATACCTGTTCTCTACTTATGATGCTGGTGATCAAATTTATGCGACTAACTTGTTATTGTCTTTGATTTTGTAGTCAATGATCCATTTGTGCTGAATTCATGGGCCAAGACATTCCCGGAGAACAAGCATGTCAAGTTCCTTGCTGATGGTTCTGCCAAATACACACATGCACTTGGGTTGGAGCTTGACCTCAGCGACAAAGGTCTCGGCATTCGCTCCAAGAGGTTTGCTCTTCTTGTTGAAGACCTGAAGGTGAAGGTTGCAAATGTTGAAGAAGGAGGAGAATACACCATCTCCGGTGCTGAAGAAATCATCAAGGCTCTTTGA
Protein sequence

>MS.gene059615.t1

MAPIKVGDVIPDGTLAFLDEENKPQSVTIHSLSAGKKVIIFAVPGAFTPTCSLKHVPGFIERSEELKGKGVDEIICISVNDPFVLNSWAKTFPENKHVKFLADGSAKYTHALGLELDLSDKGLGIRSKRFALLVEDLKVKVANVEEGGEYTISGAEEIIKAL