Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061512.t1 | XP_003620633.1 | 100 | 162 | 0 | 0 | 1 | 162 | 1 | 162 | 2.90E-86 | 327.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061512.t1 | Q9XEX2 | 73.5 | 162 | 43 | 0 | 1 | 162 | 1 | 162 | 1.1e-70 | 267.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061512.t1 | G7KPG2 | 100.0 | 162 | 0 | 0 | 1 | 162 | 1 | 162 | 2.1e-86 | 327.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059614 | MS.gene061512 | 0.882238 | 1.23E-70 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene61325 | MS.gene061512 | PPI |
| MS.gene061512 | MS.gene061510 | PPI |
| MS.gene061512 | MS.gene61324 | PPI |
| MS.gene061512 | MS.gene059615 | PPI |
| MS.gene059614 | MS.gene061512 | PPI |
| MS.gene061510 | MS.gene061512 | PPI |
| MS.gene061511 | MS.gene061512 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061512.t1 | MTR_6g087990 | 100.000 | 162 | 0 | 0 | 1 | 162 | 1 | 162 | 2.82e-116 | 326 |
| MS.gene061512.t1 | MTR_7g103610 | 55.828 | 163 | 70 | 1 | 2 | 162 | 55 | 217 | 4.25e-63 | 193 |
| MS.gene061512.t1 | MTR_7g103610 | 56.081 | 148 | 63 | 1 | 2 | 147 | 55 | 202 | 8.57e-56 | 175 |
| MS.gene061512.t1 | MTR_2g022660 | 42.446 | 139 | 79 | 1 | 22 | 159 | 58 | 196 | 5.82e-33 | 116 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061512.t1 | AT1G65980 | 73.457 | 162 | 43 | 0 | 1 | 162 | 1 | 162 | 3.92e-92 | 265 |
| MS.gene061512.t1 | AT1G65970 | 72.222 | 162 | 45 | 0 | 1 | 162 | 1 | 162 | 1.96e-90 | 260 |
| MS.gene061512.t1 | AT1G60740 | 71.605 | 162 | 46 | 0 | 1 | 162 | 1 | 162 | 1.98e-89 | 258 |
| MS.gene061512.t1 | AT3G52960 | 56.098 | 164 | 69 | 2 | 2 | 162 | 71 | 234 | 3.99e-61 | 189 |
| MS.gene061512.t1 | AT1G65990 | 58.553 | 152 | 59 | 1 | 1 | 152 | 1 | 148 | 3.72e-59 | 192 |
| MS.gene061512.t1 | AT1G65980 | 73.077 | 78 | 21 | 0 | 1 | 78 | 1 | 78 | 1.46e-39 | 129 |
| MS.gene061512.t1 | AT3G06050 | 46.032 | 126 | 67 | 1 | 35 | 159 | 73 | 198 | 1.61e-35 | 122 |
| MS.gene061512.t1 | AT3G06050 | 53.571 | 56 | 26 | 0 | 35 | 90 | 73 | 128 | 1.49e-18 | 78.6 |
Find 0 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTCTTTTGTATAAAATT+CGG | + | 43331:7121-7140 | MS.gene061512:intron | 10.0% |
| !! | AAATTCAAATTGCTTATAAT+CGG | - | 43331:8001-8020 | MS.gene061512:intergenic | 15.0% |
| !! | GTGAAAAATAAATAAATCAA+AGG | - | 43331:6773-6792 | MS.gene061512:intergenic | 15.0% |
| !!! | TCTAATTATGTTATTTTGTT+TGG | + | 43331:7268-7287 | MS.gene061512:intron | 15.0% |
| !!! | TGGAATATTTTTTTATAATG+TGG | + | 43331:7790-7809 | MS.gene061512:intron | 15.0% |
| !!! | TTTTGATTTTATTTATGTGT+TGG | + | 43331:6969-6988 | MS.gene061512:intron | 15.0% |
| !! | GTATAAAATTCGGAAATTTA+AGG | + | 43331:7131-7150 | MS.gene061512:intron | 20.0% |
| !! | GTGAAAAACAAATAAATCAA+AGG | - | 43331:6609-6628 | MS.gene061512:intergenic | 20.0% |
| !!! | ATCTATGAGAATTTTGAAAT+GGG | + | 43331:7052-7071 | MS.gene061512:intron | 20.0% |
| ! | ATAAAATTGAATCAGATCCA+TGG | + | 43331:6498-6517 | MS.gene061512:intron | 25.0% |
| ! | CAACAACAAAACAATCATTA+TGG | - | 43331:7529-7548 | MS.gene061512:intergenic | 25.0% |
| ! | TAAAATTGAATCAGATCCAT+GGG | + | 43331:6499-6518 | MS.gene061512:intron | 25.0% |
| !! | ATTAGACTGAATTAGTTTCA+TGG | + | 43331:6662-6681 | MS.gene061512:intron | 25.0% |
| !! | TTAGACTGAATTAGTTTCAT+GGG | + | 43331:6663-6682 | MS.gene061512:intron | 25.0% |
| !!! | GATCTATGAGAATTTTGAAA+TGG | + | 43331:7051-7070 | MS.gene061512:intron | 25.0% |
| !!! | GTTTTGTTTCACTTTTTACA+AGG | + | 43331:7197-7216 | MS.gene061512:intron | 25.0% |
| !!! | TTTTTAGGGTTAAGATTTGA+AGG | - | 43331:6474-6493 | MS.gene061512:intergenic | 25.0% |
| AATTACGAAGCTTGAAATCT+AGG | - | 43331:7922-7941 | MS.gene061512:intergenic | 30.0% | |
| ACAAGGTTGTCTTGATAAAA+AGG | + | 43331:7214-7233 | MS.gene061512:intron | 30.0% | |
| CAAGGTTGTCTTGATAAAAA+GGG | + | 43331:7215-7234 | MS.gene061512:intron | 30.0% | |
| CATATTCAATTCAAGAACCA+GGG | + | 43331:7608-7627 | MS.gene061512:intron | 30.0% | |
| GAAGGAAAATTGATGAAAAG+AGG | - | 43331:6456-6475 | MS.gene061512:intergenic | 30.0% | |
| GATTCAATGTCTTGCTTAAA+TGG | + | 43331:7750-7769 | MS.gene061512:intron | 30.0% | |
| TCATATTCAATTCAAGAACC+AGG | + | 43331:7607-7626 | MS.gene061512:intron | 30.0% | |
| TGAAAAGCTGCATTAAAATC+TGG | + | 43331:7958-7977 | MS.gene061512:intron | 30.0% | |
| TTTGTCATATAGATACCTGT+TGG | + | 43331:7881-7900 | MS.gene061512:intron | 30.0% | |
| ! | ATTAGACTGAATTAGCTTCA+TGG | + | 43331:6580-6599 | MS.gene061512:intron | 30.0% |
| ! | TTAGACTGAATTAGCTTCAT+GGG | + | 43331:6581-6600 | MS.gene061512:intron | 30.0% |
| !! | TTTGTTTTCTTCATCAAGGA+AGG | - | 43331:6342-6361 | MS.gene061512:intergenic | 30.0% |
| !!! | AAAGATGATGACTTTTTTGC+CGG | - | 43331:6396-6415 | MS.gene061512:intergenic | 30.0% |
| AAGGTTGCAAATGTTGAAGA+AGG | + | 43331:8294-8313 | MS.gene061512:CDS | 35.0% | |
| ATCACATCTCCTACTTTGAT+TGG | - | 43331:6302-6321 | MS.gene061512:intergenic | 35.0% | |
| ATCCATTTGTGCTGAATTCA+TGG | + | 43331:8118-8137 | MS.gene061512:CDS | 35.0% | |
| GGAAATTTAAGGATACTCTG+AGG | + | 43331:7142-7161 | MS.gene061512:intron | 35.0% | |
| GTCTTGCTTAAATGGTGATA+TGG | + | 43331:7758-7777 | MS.gene061512:intron | 35.0% | |
| TCCATTTGTGCTGAATTCAT+GGG | + | 43331:8119-8138 | MS.gene061512:CDS | 35.0% | |
| !! | GAGGTTTGTTTTCTTCATCA+AGG | - | 43331:6346-6365 | MS.gene061512:intergenic | 35.0% |
| AAAAGAGGTTAACCTGCAAG+TGG | - | 43331:6441-6460 | MS.gene061511:intergenic | 40.0% | |
| AAAGAGGTTAACCTGCAAGT+GGG | - | 43331:6440-6459 | MS.gene061511:intergenic | 40.0% | |
| AACAAGAAGAGCAAACCTCT+TGG | - | 43331:8259-8278 | MS.gene061511:intergenic | 40.0% | |
| AACTTGACATGCTTGTTCTC+CGG | - | 43331:8156-8175 | MS.gene061512:intergenic | 40.0% | |
| ACAAATCAAGTAACAAGCCC+TGG | - | 43331:7628-7647 | MS.gene061512:intergenic | 40.0% | |
| ATCAGAAGAGCTGAAAGGAA+AGG | + | 43331:7326-7345 | MS.gene061512:CDS | 40.0% | |
| CAGCATCATAAGTAGAGAAC+AGG | - | 43331:8045-8064 | MS.gene061512:intergenic | 40.0% | |
| CCAAATACACACATGCACTT+GGG | + | 43331:8193-8212 | MS.gene061512:CDS | 40.0% | |
| CTGTTCTCTACTTATGATGC+TGG | + | 43331:8043-8062 | MS.gene061512:intron | 40.0% | |
| GAATGAATGGTGACAGATTG+AGG | - | 43331:6365-6384 | MS.gene061512:intergenic | 40.0% | |
| GATGAAATCATCTGCATCAG+TGG | + | 43331:7354-7373 | MS.gene061512:CDS | 40.0% | |
| GTTGCAAATGTTGAAGAAGG+AGG | + | 43331:8297-8316 | MS.gene061512:CDS | 40.0% | |
| TAACACAGTGTATTCGCCAA+TGG | - | 43331:7836-7855 | MS.gene061512:intergenic | 40.0% | |
| TCAGAAGAGCTGAAAGGAAA+GGG | + | 43331:7327-7346 | MS.gene061512:CDS | 40.0% | |
| TCTTCTTGTTGAAGACCTGA+AGG | + | 43331:8269-8288 | MS.gene061512:CDS | 40.0% | |
| TTCTTCATCAAGGAAGGCTA+AGG | - | 43331:6336-6355 | MS.gene061512:intergenic | 40.0% | |
| ! | CTTGATGATTTCTTCAGCAC+CGG | - | 43331:8337-8356 | MS.gene061512:intergenic | 40.0% |
| ! | TAAGGATACTCTGAGGATAG+AGG | + | 43331:7149-7168 | MS.gene061512:intron | 40.0% |
| ! | TGGTGATATGGATTGAAGCA+TGG | + | 43331:7770-7789 | MS.gene061512:intron | 40.0% |
| AACAGCTTGAAGCATGTTCC+TGG | + | 43331:7291-7310 | MS.gene061512:intron | 45.0% | |
| ACTTGACATGCTTGTTCTCC+GGG | - | 43331:8155-8174 | MS.gene061512:intergenic | 45.0% | |
| ATACACACATGCACTTGGGT+TGG | + | 43331:8197-8216 | MS.gene061512:CDS | 45.0% | |
| ATTTGGCAGAACCATCAGCA+AGG | - | 43331:8179-8198 | MS.gene061512:intergenic | 45.0% | |
| CATGTCAAGTTCCTTGCTGA+TGG | + | 43331:8165-8184 | MS.gene061512:CDS | 45.0% | |
| CATTTGCAACCTTCACCTTC+AGG | - | 43331:8287-8306 | MS.gene061512:intergenic | 45.0% | |
| CCCAAGTGCATGTGTGTATT+TGG | - | 43331:8196-8215 | MS.gene061512:intergenic | 45.0% | |
| CTGAGGATAGAGGAACATCA+TGG | + | 43331:7159-7178 | MS.gene061512:intron | 45.0% | |
| GAGAGATCAGAAGAGCTGAA+AGG | + | 43331:7321-7340 | MS.gene061512:CDS | 45.0% | |
| GATACCTGTTGGATCGAGAT+TGG | + | 43331:7892-7911 | MS.gene061512:intron | 45.0% | |
| GCCAAATACACACATGCACT+TGG | + | 43331:8192-8211 | MS.gene061512:CDS | 45.0% | |
| GCCCATGAATTCAGCACAAA+TGG | - | 43331:8123-8142 | MS.gene061512:intergenic | 45.0% | |
| TAGTCCAATCTCGATCCAAC+AGG | - | 43331:7899-7918 | MS.gene061512:intergenic | 45.0% | |
| TCTGATCTCTCAATGAAGCC+AGG | - | 43331:7312-7331 | MS.gene061512:intergenic | 45.0% | |
| TGTTGAAGACCTGAAGGTGA+AGG | + | 43331:8275-8294 | MS.gene061512:CDS | 45.0% | |
| ! | CGGTGCTGAAGAAATCATCA+AGG | + | 43331:8335-8354 | MS.gene061512:CDS | 45.0% |
| !! | CGATGAATCAAAAGTGCCCA+TGG | - | 43331:6518-6537 | MS.gene061512:intergenic | 45.0% |
| !! | GTCATCATCTTTGCTGTTCC+TGG | + | 43331:6404-6423 | MS.gene061512:CDS | 45.0% |
| !!! | TCAATTTTATTTTATTTTTA+GGG | - | 43331:6488-6507 | MS.gene061512:intergenic | 5.0% |
| !!! | TTCAATTTTATTTTATTTTT+AGG | - | 43331:6489-6508 | MS.gene061512:intergenic | 5.0% |
| AACTGCAAGCTTCGTGCCAT+TGG | + | 43331:7817-7836 | MS.gene061512:intron | 50.0% | |
| ACCATTCATTCCCTCTCTGC+CGG | + | 43331:6374-6393 | MS.gene061512:CDS | 50.0% | |
| CTTGTTCTCCGGGAATGTCT+TGG | - | 43331:8145-8164 | MS.gene061512:intergenic | 50.0% | |
| GGAGGAGAATACACCATCTC+CGG | + | 43331:8315-8334 | MS.gene061512:CDS | 50.0% | |
| GTAGGAGATGTGATCCCAGA+TGG | + | 43331:6308-6327 | MS.gene061512:CDS | 50.0% | |
| TAACCTGCAAGTGGGTGTGA+AGG | - | 43331:6432-6451 | MS.gene061512:intergenic | 50.0% | |
| TTCATGGGCCAAGACATTCC+CGG | + | 43331:8134-8153 | MS.gene061512:CDS | 50.0% | |
| ! | GATTTCTTCAGCACCGGAGA+TGG | - | 43331:8331-8350 | MS.gene061512:intergenic | 50.0% |
| !!! | GACTTTTTTGCCGGCAGAGA+GGG | - | 43331:6387-6406 | MS.gene061512:intergenic | 50.0% |
| !!! | TGACTTTTTTGCCGGCAGAG+AGG | - | 43331:6388-6407 | MS.gene061512:intergenic | 50.0% |
| GACCTCAGCGACAAAGGTCT+CGG | + | 43331:8225-8244 | MS.gene061512:CDS | 55.0% | |
| GAGCTTGACCTCAGCGACAA+AGG | + | 43331:8219-8238 | MS.gene061512:CDS | 55.0% | |
| ! | AGGAAGGCTAAGGTGCCATC+TGG | - | 43331:6326-6345 | MS.gene061512:intergenic | 55.0% |
| ! | GGAAGGCTAAGGTGCCATCT+GGG | - | 43331:6325-6344 | MS.gene061512:intergenic | 55.0% |
| GCCGGCAGAGAGGGAATGAA+TGG | - | 43331:6378-6397 | MS.gene061512:intergenic | 60.0% | |
| GTCTCGGCATTCGCTCCAAG+AGG | + | 43331:8241-8260 | MS.gene061512:CDS | 60.0% | |
| GTGCCTTCACACCCACTTGC+AGG | + | 43331:6426-6445 | MS.gene061512:CDS | 60.0% | |
| TGCCGAGACCTTTGTCGCTG+AGG | - | 43331:8230-8249 | MS.gene061512:intergenic | 60.0% | |
| !! | CAAGTGGGTGTGAAGGCACC+AGG | - | 43331:6425-6444 | MS.gene061512:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43331 | gene | 6293 | 8365 | 6293 | ID=MS.gene061512 |
| 43331 | mRNA | 6293 | 8365 | 6293 | ID=MS.gene061512.t1;Parent=MS.gene061512 |
| 43331 | exon | 6293 | 6447 | 6293 | ID=MS.gene061512.t1.exon1;Parent=MS.gene061512.t1 |
| 43331 | CDS | 6293 | 6447 | 6293 | ID=cds.MS.gene061512.t1;Parent=MS.gene061512.t1 |
| 43331 | exon | 7296 | 7375 | 7296 | ID=MS.gene061512.t1.exon2;Parent=MS.gene061512.t1 |
| 43331 | CDS | 7296 | 7375 | 7296 | ID=cds.MS.gene061512.t1;Parent=MS.gene061512.t1 |
| 43331 | exon | 8112 | 8365 | 8112 | ID=MS.gene061512.t1.exon3;Parent=MS.gene061512.t1 |
| 43331 | CDS | 8112 | 8365 | 8112 | ID=cds.MS.gene061512.t1;Parent=MS.gene061512.t1 |
| Gene Sequence |
| Protein sequence |
