AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059684


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059684.t1 MTR_5g014110 74.894 235 41 5 43 262 119 350 5.30e-110 322
MS.gene059684.t1 MTR_5g014110 100.000 44 0 0 1 44 22 65 2.60e-21 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 57 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTTGGCAGTGAATAAAAT+TGG 0.189398 5.1:-6960125 MS.gene059684:CDS
TTCACCTTGTGTTGGTGTTA+TGG 0.239203 5.1:-6960237 MS.gene059684:CDS
GAATGTTGAGAAGGAGAATA+AGG 0.275772 5.1:-6960003 MS.gene059684:CDS
GGGTTGTTGAATATTGTTAT+TGG 0.296129 5.1:+6960803 None:intergenic
GGGGTTGAAAATGCCATATT+TGG 0.313158 5.1:-6960141 MS.gene059684:CDS
TTTCCTGAAGGGTGTTCTTC+CGG 0.343308 5.1:-6960374 MS.gene059684:intron
AAGCAGAAGCAGAAGCATTC+AGG 0.352808 5.1:-6959924 MS.gene059684:CDS
GGTGAGAGCTTTCAAAGTTT+AGG 0.356862 5.1:-6959903 MS.gene059684:CDS
GAAGGAGATGAAAATGGATT+TGG 0.357391 5.1:-6960027 MS.gene059684:CDS
ATTGTTGAATGAGATGAAAA+TGG 0.365578 5.1:-6959970 MS.gene059684:CDS
CTCTCCCTCAAATCCGGTTT+AGG 0.377684 5.1:-6960629 MS.gene059684:intron
TTCTCTCTCTCCCTCAAATC+CGG 0.384397 5.1:-6960635 MS.gene059684:CDS
ACAACGTTGGTGTTGGTGTT+GGG 0.388448 5.1:-6960298 MS.gene059684:CDS
CTTTCAACAAATTTCCCAAC+TGG 0.401458 5.1:-6960722 MS.gene059684:CDS
TGAATAAAATTGGGTTGGAT+AGG 0.403566 5.1:-6960115 MS.gene059684:CDS
ATTTGGCAGTGAATAAAATT+GGG 0.410901 5.1:-6960124 MS.gene059684:CDS
GGTGCTGAAGGAGATGAAAA+TGG 0.412755 5.1:-6960033 MS.gene059684:CDS
AGAAGTTGAGTAGTTATTTG+TGG 0.420121 5.1:+6960760 None:intergenic
CTGTTATAGTTGAAAGTAAA+GGG 0.420993 5.1:+6960783 None:intergenic
CGGTTGGCAAAATTTGAACT+TGG 0.433091 5.1:-6960354 MS.gene059684:CDS
ATGAAAATGGGAGTTTCTAA+AGG 0.436835 5.1:-6959957 MS.gene059684:CDS
AATTTGAACTTGGAACCATT+TGG 0.450192 5.1:-6960344 MS.gene059684:CDS
GTTGGTGTTGGTGTTGGGGT+TGG 0.452126 5.1:-6960293 MS.gene059684:CDS
TAATTGTGAAGGTGATTTGC+AGG 0.455443 5.1:-6960054 MS.gene059684:CDS
CTGAAGGGTGTTCTTCCGGT+TGG 0.461829 5.1:-6960370 MS.gene059684:intron
GGCAGTGAATAAAATTGGGT+TGG 0.463812 5.1:-6960120 MS.gene059684:CDS
TGGAGAGTAAGAGAGTTTGA+AGG 0.467991 5.1:+6960694 None:intergenic
GTTTGAAGGTTGGACCAGTT+GGG 0.475548 5.1:+6960708 None:intergenic
AGTTTGAAGGTTGGACCAGT+TGG 0.477278 5.1:+6960707 None:intergenic
GAATAAAATTGGGTTGGATA+GGG 0.477630 5.1:-6960114 MS.gene059684:CDS
TTGTTGTTATTGTGACCAAA+TGG 0.479560 5.1:+6960329 None:intergenic
GCTGTTATAGTTGAAAGTAA+AGG 0.481381 5.1:+6960782 None:intergenic
AACAACAACCCTTGTGTCAC+CGG 0.481710 5.1:+6960200 None:intergenic
AAGACCTAAACCGGATTTGA+GGG 0.482511 5.1:+6960625 None:intergenic
CAACGTTGGTGTTGGTGTTG+GGG 0.488480 5.1:-6960297 MS.gene059684:CDS
GAAGAAGTTGTGGTTGTGGT+TGG 0.496533 5.1:+6960674 None:intergenic
AAAGACCTAAACCGGATTTG+AGG 0.506868 5.1:+6960624 None:intergenic
GATTTGAGGGAGAGAGAGAA+AGG 0.514169 5.1:+6960638 None:intergenic
AATGGGAGTTTCTAAAGGGA+AGG 0.515092 5.1:-6959952 MS.gene059684:CDS
AACAACGTTGGTGTTGGTGT+TGG 0.518386 5.1:-6960299 MS.gene059684:CDS
TTGTTGAATGAGATGAAAAT+GGG 0.539773 5.1:-6959969 MS.gene059684:CDS
TGAAAATGGGAGTTTCTAAA+GGG 0.540370 5.1:-6959956 MS.gene059684:CDS
TGTGGTCTTTCACCTTGTGT+TGG 0.556198 5.1:-6960245 MS.gene059684:CDS
TATGGCTAGGACGGTGATGC+CGG 0.560278 5.1:-6960219 MS.gene059684:CDS
ACGGTGATGCCGGTGACACA+AGG 0.562748 5.1:-6960209 MS.gene059684:CDS
AAGAAATGAAGAAAGATGAG+TGG 0.566368 5.1:-6960088 MS.gene059684:CDS
AGAAGAAGAAGTTGTGGTTG+TGG 0.568173 5.1:+6960670 None:intergenic
GGATTTGGAGAATGTTGAGA+AGG 0.568307 5.1:-6960012 MS.gene059684:CDS
AACAACAACAACGTTGGTGT+TGG 0.570170 5.1:-6960305 MS.gene059684:CDS
CTTGTGTTGGTGTTATGGCT+AGG 0.572621 5.1:-6960232 MS.gene059684:CDS
GAGTAAGAGAGTTTGAAGGT+TGG 0.580998 5.1:+6960698 None:intergenic
AGGTGATTTGCAGGTGCTGA+AGG 0.592161 5.1:-6960045 MS.gene059684:CDS
TGTTGGTGTTATGGCTAGGA+CGG 0.624391 5.1:-6960228 MS.gene059684:CDS
TCACTCACTACAATATTCTG+AGG 0.628975 5.1:+6959831 None:intergenic
AACAACGATGATAATTGTGA+AGG 0.673292 5.1:-6960065 MS.gene059684:CDS
CTAGCCATAACACCAACACA+AGG 0.698523 5.1:+6960233 None:intergenic
CGGTGATGCCGGTGACACAA+GGG 0.700522 5.1:-6960208 MS.gene059684:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGTTTAGGAAGCAATAAAAA+TGG - chr5.1:6960709-6960728 MS.gene059684:CDS 25.0%
! ATTGTTGAATGAGATGAAAA+TGG - chr5.1:6960627-6960646 MS.gene059684:intron 25.0%
! ATTTGGCAGTGAATAAAATT+GGG - chr5.1:6960473-6960492 MS.gene059684:intron 25.0%
! CTGTTATAGTTGAAAGTAAA+GGG + chr5.1:6959817-6959836 None:intergenic 25.0%
! TATTTGGCAGTGAATAAAAT+TGG - chr5.1:6960472-6960491 MS.gene059684:intron 25.0%
! TTAAAGTGTAAAGAGAAAGA+AGG + chr5.1:6960079-6960098 None:intergenic 25.0%
! TTGTTGAATGAGATGAAAAT+GGG - chr5.1:6960628-6960647 MS.gene059684:intron 25.0%
!! ATTTTATTCACTGCCAAATA+TGG + chr5.1:6960472-6960491 None:intergenic 25.0%
AACAACGATGATAATTGTGA+AGG - chr5.1:6960532-6960551 MS.gene059684:intron 30.0%
AAGAAATGAAGAAAGATGAG+TGG - chr5.1:6960509-6960528 MS.gene059684:intron 30.0%
AAGAGAGAAATGACCAAATT+GGG + chr5.1:6960104-6960123 None:intergenic 30.0%
AATTTGAACTTGGAACCATT+TGG - chr5.1:6960253-6960272 MS.gene059684:CDS 30.0%
AGAAGAAAGAGAGAAATTAG+CGG + chr5.1:6960032-6960051 None:intergenic 30.0%
AGAAGTTGAGTAGTTATTTG+TGG + chr5.1:6959840-6959859 None:intergenic 30.0%
ATGAAAATGGGAGTTTCTAA+AGG - chr5.1:6960640-6960659 MS.gene059684:CDS 30.0%
GCTAAAAACAGTGAAAAATG+TGG - chr5.1:6960334-6960353 MS.gene059684:CDS 30.0%
GCTGTTATAGTTGAAAGTAA+AGG + chr5.1:6959818-6959837 None:intergenic 30.0%
GGGTTGTTGAATATTGTTAT+TGG + chr5.1:6959797-6959816 None:intergenic 30.0%
GTTTCACCTCAAATTGAAAA+GGG - chr5.1:6960190-6960209 MS.gene059684:CDS 30.0%
TGAAAATGGGAGTTTCTAAA+GGG - chr5.1:6960641-6960660 MS.gene059684:CDS 30.0%
TGTTTCACCTCAAATTGAAA+AGG - chr5.1:6960189-6960208 MS.gene059684:CDS 30.0%
TTACACTTTAAACCCCAATT+TGG - chr5.1:6960088-6960107 MS.gene059684:CDS 30.0%
! GAGTGATTTTGAATCTGAAT+TGG - chr5.1:6960783-6960802 MS.gene059684:CDS 30.0%
! GCTAGAATTAGTAACAGTTT+TGG + chr5.1:6960161-6960180 None:intergenic 30.0%
! TTGTTGTTATTGTGACCAAA+TGG + chr5.1:6960271-6960290 None:intergenic 30.0%
!! ATTAGTAACAGTTTTGGTGT+TGG + chr5.1:6960155-6960174 None:intergenic 30.0%
!! GAATAAAATTGGGTTGGATA+GGG - chr5.1:6960483-6960502 MS.gene059684:intron 30.0%
!! GTTGTTGTTGAATTTTCGTT+GGG - chr5.1:6960411-6960430 MS.gene059684:intron 30.0%
!! TGAATAAAATTGGGTTGGAT+AGG - chr5.1:6960482-6960501 MS.gene059684:intron 30.0%
!! TTGTTGTTGAATTTTCGTTG+GGG - chr5.1:6960412-6960431 MS.gene059684:intron 30.0%
!!! TTTCACTGTTTTTAGCATCT+GGG + chr5.1:6960330-6960349 None:intergenic 30.0%
!!! TTTTCACTGTTTTTAGCATC+TGG + chr5.1:6960331-6960350 None:intergenic 30.0%
AAAGAGAAAGAAGGAAGAAG+AGG + chr5.1:6960070-6960089 None:intergenic 35.0%
AACAACAACAACAACAACGT+TGG - chr5.1:6960286-6960305 MS.gene059684:CDS 35.0%
AAGACAAGAGGAGAATGATT+CGG + chr5.1:6960007-6960026 None:intergenic 35.0%
AAGAGAAAGAAGGAAGAAGA+GGG + chr5.1:6960069-6960088 None:intergenic 35.0%
AGAAGAAGAAGAAGAAGTTG+TGG + chr5.1:6959936-6959955 None:intergenic 35.0%
AGAGAGAAATGACCAAATTG+GGG + chr5.1:6960103-6960122 None:intergenic 35.0%
CTTTCAACAAATTTCCCAAC+TGG - chr5.1:6959875-6959894 MS.gene059684:CDS 35.0%
GAAGAGAGAAATGACCAAAT+TGG + chr5.1:6960105-6960124 None:intergenic 35.0%
GAATGTTGAGAAGGAGAATA+AGG - chr5.1:6960594-6960613 MS.gene059684:intron 35.0%
TCACTCACTACAATATTCTG+AGG + chr5.1:6960769-6960788 None:intergenic 35.0%
TGAACCAAAAAGACCTAAAC+CGG + chr5.1:6959984-6960003 None:intergenic 35.0%
! AAATCCGGTTTAGGTCTTTT+TGG - chr5.1:6959977-6959996 MS.gene059684:CDS 35.0%
! ACACAACCCTTTTCAATTTG+AGG + chr5.1:6960199-6960218 None:intergenic 35.0%
! ATTTGAGGTGAAACAGAAAG+AGG + chr5.1:6960184-6960203 None:intergenic 35.0%
! CAAGTTCAAATTTTGCCAAC+CGG + chr5.1:6960245-6960264 None:intergenic 35.0%
! GAAGGAGATGAAAATGGATT+TGG - chr5.1:6960570-6960589 MS.gene059684:intron 35.0%
! TAATTGTGAAGGTGATTTGC+AGG - chr5.1:6960543-6960562 MS.gene059684:intron 35.0%
! TTTGAGGTGAAACAGAAAGA+GGG + chr5.1:6960183-6960202 None:intergenic 35.0%
!! GGTTGTTGTTGAATTTTCGT+TGG - chr5.1:6960410-6960429 MS.gene059684:intron 35.0%
AAAGACCTAAACCGGATTTG+AGG + chr5.1:6959976-6959995 None:intergenic 40.0%
AAATTAGCGGAGAAGACAAG+AGG + chr5.1:6960019-6960038 None:intergenic 40.0%
AACAACAACAACGTTGGTGT+TGG - chr5.1:6960292-6960311 MS.gene059684:CDS 40.0%
AAGACCTAAACCGGATTTGA+GGG + chr5.1:6959975-6959994 None:intergenic 40.0%
AGAAGAAGAAGTTGTGGTTG+TGG + chr5.1:6959930-6959949 None:intergenic 40.0%
AGAGAAAGAAGGAAGAAGAG+GGG + chr5.1:6960068-6960087 None:intergenic 40.0%
CGGTTGGCAAAATTTGAACT+TGG - chr5.1:6960243-6960262 MS.gene059684:CDS 40.0%
GAGTAAGAGAGTTTGAAGGT+TGG + chr5.1:6959902-6959921 None:intergenic 40.0%
GGATTTGGAGAATGTTGAGA+AGG - chr5.1:6960585-6960604 MS.gene059684:intron 40.0%
GGCAGTGAATAAAATTGGGT+TGG - chr5.1:6960477-6960496 MS.gene059684:intron 40.0%
GGGGTTGAAAATGCCATATT+TGG - chr5.1:6960456-6960475 MS.gene059684:intron 40.0%
GGTGAGAGCTTTCAAAGTTT+AGG - chr5.1:6960694-6960713 MS.gene059684:CDS 40.0%
GGTGTAAGAATCTGAGAGAA+CGG + chr5.1:6960134-6960153 None:intergenic 40.0%
TGGAGAGTAAGAGAGTTTGA+AGG + chr5.1:6959906-6959925 None:intergenic 40.0%
! AATGGGAGTTTCTAAAGGGA+AGG - chr5.1:6960645-6960664 MS.gene059684:CDS 40.0%
! GTTGTGTTCCTTTTCCTGAA+GGG - chr5.1:6960212-6960231 MS.gene059684:CDS 40.0%
! TGTGAGTTTTCCAGGGAAAT+CGG - chr5.1:6960435-6960454 MS.gene059684:intron 40.0%
!! TTCACCTTGTGTTGGTGTTA+TGG - chr5.1:6960360-6960379 MS.gene059684:CDS 40.0%
AACAACAACCCTTGTGTCAC+CGG + chr5.1:6960400-6960419 None:intergenic 45.0%
AGTTTGAAGGTTGGACCAGT+TGG + chr5.1:6959893-6959912 None:intergenic 45.0%
CTAGCCATAACACCAACACA+AGG + chr5.1:6960367-6960386 None:intergenic 45.0%
GAAGAACACCCTTCAGGAAA+AGG + chr5.1:6960223-6960242 None:intergenic 45.0%
GAAGAAGTTGTGGTTGTGGT+TGG + chr5.1:6959926-6959945 None:intergenic 45.0%
GATTTGAGGGAGAGAGAGAA+AGG + chr5.1:6959962-6959981 None:intergenic 45.0%
GTTTGAAGGTTGGACCAGTT+GGG + chr5.1:6959892-6959911 None:intergenic 45.0%
TGTGGTCTTTCACCTTGTGT+TGG - chr5.1:6960352-6960371 MS.gene059684:CDS 45.0%
TTCTCTCTCTCCCTCAAATC+CGG - chr5.1:6959962-6959981 MS.gene059684:CDS 45.0%
! ATTTTCAACCCCGATTTCCC+TGG + chr5.1:6960448-6960467 None:intergenic 45.0%
! GGTGCTGAAGGAGATGAAAA+TGG - chr5.1:6960564-6960583 MS.gene059684:intron 45.0%
! GGTTGTGTTCCTTTTCCTGA+AGG - chr5.1:6960211-6960230 MS.gene059684:CDS 45.0%
! GTGAGTTTTCCAGGGAAATC+GGG - chr5.1:6960436-6960455 MS.gene059684:intron 45.0%
! TGAGTTTTCCAGGGAAATCG+GGG - chr5.1:6960437-6960456 MS.gene059684:intron 45.0%
!! AACAACGTTGGTGTTGGTGT+TGG - chr5.1:6960298-6960317 MS.gene059684:CDS 45.0%
!! AAGCAGAAGCAGAAGCATTC+AGG - chr5.1:6960673-6960692 MS.gene059684:CDS 45.0%
!! ACAACGTTGGTGTTGGTGTT+GGG - chr5.1:6960299-6960318 MS.gene059684:CDS 45.0%
!! CTTGTGTTGGTGTTATGGCT+AGG - chr5.1:6960365-6960384 MS.gene059684:CDS 45.0%
!! TGTTGGTGTTATGGCTAGGA+CGG - chr5.1:6960369-6960388 MS.gene059684:intron 45.0%
!! TTTCCTGAAGGGTGTTCTTC+CGG - chr5.1:6960223-6960242 MS.gene059684:CDS 45.0%
AGGAAGAAGAGGGGTAGAAG+AGG + chr5.1:6960059-6960078 None:intergenic 50.0%
CTCTCCCTCAAATCCGGTTT+AGG - chr5.1:6959968-6959987 MS.gene059684:CDS 50.0%
! GTTGGGGTGTGAGTTTTCCA+GGG - chr5.1:6960428-6960447 MS.gene059684:intron 50.0%
!! AGGTGATTTGCAGGTGCTGA+AGG - chr5.1:6960552-6960571 MS.gene059684:intron 50.0%
!! CAACGTTGGTGTTGGTGTTG+GGG - chr5.1:6960300-6960319 MS.gene059684:CDS 50.0%
CAACCGGAAGAACACCCTTC+AGG + chr5.1:6960229-6960248 None:intergenic 55.0%
TATGGCTAGGACGGTGATGC+CGG - chr5.1:6960378-6960397 MS.gene059684:intron 55.0%
! CGTTGGGGTGTGAGTTTTCC+AGG - chr5.1:6960427-6960446 MS.gene059684:intron 55.0%
!! CTGAAGGGTGTTCTTCCGGT+TGG - chr5.1:6960227-6960246 MS.gene059684:CDS 55.0%
!! GTTGGTGTTGGTGTTGGGGT+TGG - chr5.1:6960304-6960323 MS.gene059684:CDS 55.0%
ACGGTGATGCCGGTGACACA+AGG - chr5.1:6960388-6960407 MS.gene059684:intron 60.0%
CGGTGATGCCGGTGACACAA+GGG - chr5.1:6960389-6960408 MS.gene059684:intron 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 6959779 6960840 6959779 ID=MS.gene059684
chr5.1 mRNA 6959779 6960840 6959779 ID=MS.gene059684.t1;Parent=MS.gene059684
chr5.1 exon 6960630 6960840 6960630 ID=MS.gene059684.t1.exon1;Parent=MS.gene059684.t1
chr5.1 CDS 6960630 6960840 6960630 ID=cds.MS.gene059684.t1;Parent=MS.gene059684.t1
chr5.1 exon 6959779 6960386 6959779 ID=MS.gene059684.t1.exon2;Parent=MS.gene059684.t1
chr5.1 CDS 6959779 6960386 6959779 ID=cds.MS.gene059684.t1;Parent=MS.gene059684.t1
Gene Sequence

>MS.gene059684

ATGACAGCAAAAGTACCAATAACAATATTCAACAACCCTTTACTTTCAACTATAACAGCCACAAATAACTACTCAACTTCTGATTTTTCATTCTCACTTTCAACAAATTTCCCAACTGGTCCAACCTTCAAACTCTCTTACTCTCCAACCACAACCACAACTTCTTCTTCTTCTTCTCTTCCTTTCTCTCTCTCCCTCAAATCCGGTTTAGGTCTTTTTGGTTCACCGAATCATTCTCCTCTTGTCTTCTCCGCTAATTTCTCTCTTTCTTCTCTTTCCTCTTCTACCCCTCTTCTTCCTTCTTTCTCTTTACACTTTAAACCCCAATTTGGTCATTTCTCTCTTCACAAAACCGTTCTCTCAGATTCTTACACCAACACCAAAACTGTTACTAATTCTAGCCCTCTTTCTGTTTCACCTCAAATTGAAAAGGGTTGTGTTCCTTTTCCTGAAGGGTGTTCTTCCGGTTGGCAAAATTTGAACTTGGAACCATTTGGTCACAATAACAACAACAACAACAACAACGTTGGTGTTGGTGTTGGGGTTGGCCCAGATGCTAAAAACAGTGAAAAATGTGGTCTTTCACCTTGTGTTGGTGTTATGGCTAGGACGGTGATGCCGGTGACACAAGGGTTGTTGTTGAATTTTCGTTGGGGTGTGAGTTTTCCAGGGAAATCGGGGTTGAAAATGCCATATTTGGCAGTGAATAAAATTGGGTTGGATAGGGTTGAAGAAATGAAGAAAGATGAGTGGAACAACGATGATAATTGTGAAGGTGATTTGCAGGTGCTGAAGGAGATGAAAATGGATTTGGAGAATGTTGAGAAGGAGAATAAGGAGATGAAGAGATTGTTGAATGAGATGAAAATGGGAGTTTCTAAAGGGAAGGTTTCAAAGCAGAAGCAGAAGCATTCAGGTGAGAGCTTTCAAAGTTTAGGAAGCAATAAAAATGGAAGAAAAGAGAATGAGAAAAAACAAGAAATCAAGCCTCAGAATATTGTAGTGAGTGATTTTGAATCTGAATTGGAAAAAGCTATAAAAGCTGCTGCTGCTTCTTCTTAG

Protein sequence

>MS.gene059684.t1

MTAKVPITIFNNPLLSTITATNNYSTSDFSFSLSTNFPTGPTFKLSYSPTTTTTSSSSSLPFSLSLKSGLGCSSGWQNLNLEPFGHNNNNNNNNVGVGVGVGPDAKNSEKCGLSPCVGVMARTVMPVTQGLLLNFRWGVSFPGKSGLKMPYLAVNKIGLDRVEEMKKDEWNNDDNCEGDLQVLKEMKMDLENVEKENKEMKRLLNEMKMGVSKGKVSKQKQKHSGESFQSLGSNKNGRKENEKKQEIKPQNIVVSDFESELEKAIKAAAASS